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Impact of soaking, sprouting on antioxidant and anti-nutritional factors in millet grains 浸泡、发芽对谷子籽粒抗氧化和抗营养因子的影响
Pub Date : 2020-10-09 DOI: 10.25081/jp.2020.v12.6384
G. Bhuvaneshwari, A. Nirmalakumari, S. Kalaiselvi
Commonly consumed millet types viz., Little millet, foxtail millet, pearl millet, finger millet, and Kodo millet were tested under two processing methods of soaking and germination at a different time point. Most popular processing practices adopted by Indian households were studied for their influence on the biochemical properties, antioxidant profile, and anti-nutritive factors. The results showed that the sprouting process showed a maximum influence on the antioxidant and anti-nutritive factors. The comparative analysis of the five millets suggested 24h soaking and 24h germination was found to be best for producing nutritionally enriched millet products. Tannin content decreases with an increase in germination. The reduction in tannin was about 50% in little millet (0.347 mg/g) and the highest was recorded in finger millet (2.07 mg/g). The highest amount of saponin content was found in pearl millet( 39.53 mg/g) followed by finger millet (34.86 mg/g) with 24 hr soaking and 24 hr germination. The phytase activity was found to be higher in little millet (61.520 u/kg) when prolonged soaking and germination. The DPPH assay showed sprouted foxtail millet grains contain more antioxidant activity (81.13%) with extended period of soaking and germination.
对常用的小小米、谷子、珍珠小米、手指小米和古多小米进行了浸泡和不同时间点发芽两种加工方法的试验。研究了印度家庭采用的最流行的加工方法对生物化学特性、抗氧化特性和抗营养因子的影响。结果表明,发芽过程对抗氧化和抗营养因子的影响最大。通过对5种谷子的比较分析,发现浸泡24h和萌发24h最有利于生产营养丰富的谷子制品。单宁含量随发芽期的增加而降低。小谷子单宁含量降低约50% (0.347 mg/g),以指谷子最高(2.07 mg/g)。浸泡24 h、萌发24 h时,珍珠粟皂苷含量最高(39.53 mg/g),其次是指粟(34.86 mg/g)。小谷子浸泡和萌发时间越长,植酸酶活性越高(61.520 u/kg)。DPPH试验结果表明,随着浸泡时间的延长和萌发时间的延长,谷子发芽后籽粒抗氧化活性提高,达到81.13%。
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引用次数: 3
Establishment of callus and cell suspension culture of Sophora alopecuroides Linn. for the production of oxymatrine 苦豆子愈伤组织的建立及细胞悬浮培养。用于生产氧化苦参碱
Pub Date : 2020-09-01 DOI: 10.25081/jp.2020.v12.6308
M. Tsolmon, G. Oyundari, O. Yu, K. Senthil
Oxymatrine is one of the most important biologically active compound and is present in Sophora alopecuroides L. The present investigation focuses on the development of an efficient tissue culture method to induce callus and cell suspension culture of S. alopecuroides by studying the effect of jasmonic acid and nitric oxide on cell suspension culture. Callus induction efficiency is high in axenic leaf explants grown in MS medium supplemented with 1.0 mg/L Kinetin (Kin), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The cell suspension culture was developed using the same callus induction medium without agar. The maximum cell number and dry weight of suspension culture were obtained by the 9th day of incubation. The synthesis of oxymatrine is higher in jasmonic acid and nitric oxide (200 μMJA and 50 μMNO) combination (11.91 μg/g) when compared to the non-elicited control (8.3 μg/g ) of callus.
氧化苦参碱是苦豆子中最重要的生物活性化合物之一,本研究通过研究茉莉酸和一氧化氮对苦豆子细胞悬浮培养的影响,建立一种高效的组织培养方法诱导愈伤组织和细胞悬浮培养。在添加1.0 mg/L Kinetin (Kin)和1.0 mg/L 2,4-二氯苯氧乙酸(2,4- d)的MS培养基中培养的无性系叶片愈伤组织诱导效率较高。采用不含琼脂的相同愈伤组织诱导培养基进行细胞悬浮培养。在培养第9天时,悬浮培养细胞数和干重达到最大值。茉莉酸和一氧化氮(200 μMJA和50 μMNO)组合下,氧化苦参碱的合成(11.91 μg/g)高于非诱导对照(8.3 μg/g)。
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引用次数: 3
Characterization of Little millet (Panicum sumatrense) varieties using Morphological descriptors and SSR based DNA fingerprinting 基于形态描述子和SSR DNA指纹图谱的小粟(Panicum sumatrense)品种鉴定
Pub Date : 2020-08-12 DOI: 10.25081/jp.2020.v12.6317
Senthilkumar A. Natesan, Gayathri Venugopalan, S. Selvamani, Madhumitha Krishnamoorthy, Sarankumar Chandran, Nirmalakumari Angamuthu
Little millet (Panicum sumatrense) is also known as Indian millet. It is cultivated as a cereal across Nepal, India and Western Myanmar, and its center of origin is West Africa. The wild relative of little millet is P.psilopodium. It forms an important role in tribal agriculture in Eastern Ghats of India. Little millet is grown on temperate and tropical climate. Little millet is an annual tuffed grass with slender culms, soft leaves, inflorescence a panicle with erect hairy branches, spikelets in pairs with two glumes. The discovery of syntenic regions among the cereals which aids to identify useful alleles of important agromorphological traits. An earlier study by Ali et al [2] developed 48 EST-SSR markers among 37 accessions of the little millets. Only limited reports are available on the genetic diversity of little millet germplasm, that too is based on a limited number of DNA markers [3, 4]. Thus the limited sequence information available in the little millets necessitates the search for the available markers from the maize, barnyard millet, and pearl millet due to the cereal millet synteny and also the availability of the SSR markers. The little millet husked grain is cooked as like rice and sometimes made into flour for different types of food preparations. The soft straw is palatable to cattle and the green plant has potentialities as a quick-growing fodder. The present investigation was conducted to carry out the comprehensive characterization of little millet genotypes based on Distinctness, Uniformity and Stability (DUS) characters for Protection of Plant Varieties and Farmers Rights Authority (PPVFRA) registration because of its property to identify the dissimilarity between the newly released and the existing genotypes as well as to distinguish the germplasm. Cereal crops exhibit cross-genera transferability, of DNA markers into many cereals such as maize, Characterization of little millet (Panicum sumatrense) varieties using morphological descriptors and SSR based DNA fingerprinting
小小米(Panicum sumatrense)也被称为印度小米。它作为一种谷物在尼泊尔、印度和缅甸西部种植,它的起源中心是西非。小小米的野生近亲是psilopodium。它在印度东高止山脉的部落农业中起着重要作用。小小米生长在温带和热带气候。小小米是一种一年生丛生的草,有纤细的秆,柔软的叶,花序一圆锥花序具直立有毛的枝,小穗成对具二颖片。在谷物中发现共合区,有助于鉴定重要农业形态性状的有用等位基因。Ali等[2]早前在37份小小米材料中开发出48个EST-SSR标记。关于小小米种质资源遗传多样性的报道有限,也是基于有限数量的DNA标记[3,4]。因此,由于小谷子的序列信息有限,需要从玉米、稗子和珍珠谷子中寻找可用的标记,这是由于谷子的同质性和SSR标记的可用性。这种带壳的小谷子像大米一样被煮熟,有时被制成面粉,用于制作不同类型的食物。软稻草对牛来说是可口的,绿色植物有潜力成为速生饲料。利用小谷子基因型的显著性、均匀性和稳定性(DUS)特征,利用其在植物品种保护和农民权利管理局(PPVFRA)注册中的特性,对新发布的小谷子基因型和现有小谷子基因型进行综合鉴定,并对其进行种质鉴别。谷类作物的DNA标记具有跨属可转移性,如玉米。小粟(Panicum sumatrense)品种的形态描述子和基于SSR的DNA指纹鉴定
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引用次数: 3
The essential oil constituents of Artabotrys species – A review 木树属植物精油成分研究进展
Pub Date : 2020-07-27 DOI: 10.25081/jp.2020.v12.6332
R. Subban, S. Kaveri
Artabotrys species which belongs to Annonaceae family are pleasant smelling and it is attributed to the presence of mono and sesquiterpenoids present in the essential oil of the plant. The objective of the present work is to review the chemical composition of the essential oils reported from twenty different Artabotrys species from various parts of the world. In the various Artabotrys species, the major compounds are monoterpene and sesquiterpene hydrocarbons and oxygenated sesquiterpenes. The frequently and most commonly identified constituents are β-caryophyllene, caryophyllene oxide, 3-Carene, cyperene, cyperenone and 1,5-epoxy-salvial4(14)-ene. Other constituents seems to be more specific to the respective Artabotrys species.
番荔枝科番荔枝属植物具有良好的气味,这是由于其精油中含有单萜类和倍半萜类化合物。本文对来自世界各地的20种不同的紫檀属植物精油的化学成分进行了综述。在各种树属植物中,主要化合物是单萜和倍半萜烃和氧化倍半萜。最常见和最常见的鉴定成分是β-石竹烯、石竹烯氧化物、3-蒈烯、cyperene、cyperenone和1,5-环氧-salvial4(14)-ene。其他成分似乎更具体到各自的树属物种。
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引用次数: 2
Seed priming effects on germination and first growth of the medicinal plant Achillea millefolium L. 引种对药用植物千叶阿喀巴发芽和初生的影响。
Pub Date : 2020-07-11 DOI: 10.25081/jp.2020.v12.6324
P. Kanatas, Vyronas Dellaportas, I. Kakabouki, Panayiota Papastylianou
This study evaluated the effects of seed priming on germination and growth of A. millefolium by means of laboratory and greenhouse experiments conducted during 2018 in Agricultural University of Athens. Treatments were GA3 (400 and 800 ppm), potassium nitrate (2% and 4%), polyethylene-glycol (soaking for 12 and 24h) besides an untreated control. Experiment in Petri dishes revealed that GA3 at 400 ppm, potassium nitrate (at concentration 2 and 4%) and PEG significantly increased germination percentage of A. millefolium, while germination rate was also significantly improved as a result of all seed priming techniques. In addition, due to the soil experiment, seedling emergence was significantly increased by GA3 at 400 ppm, potassium nitrate (at both concentrations) and PEG compared with the untreated seeds. Dry biomass of the young seedlings was significantly enhanced by means of GA3 (at 400 and 800 ppm), KNO3 (4%) and PEG for 24 h, indicating the potential effect of seed priming on first growth as well. The results of the present study revealed the significant positive effects of seed priming on A. millefolium seed germination, seedling emergence and early growth.
本研究通过2018年在雅典农业大学进行的室内和温室试验,评估了种子激发对千叶草发芽和生长的影响。除未处理对照外,处理分别为GA3(400和800 ppm)、硝酸钾(2%和4%)、聚乙二醇(浸泡12和24h)。培养皿试验结果表明,400 ppm的GA3、浓度为2和4%的硝酸钾和PEG均能显著提高千叶草的发芽率,同时各引种技术均能显著提高千叶草的发芽率。此外,由于土壤试验,与未处理种子相比,400 ppm GA3、硝酸钾(两种浓度)和PEG显著提高了幼苗出苗率。GA3 (400 ppm和800 ppm)、KNO3(4%)和PEG处理24 h后,幼苗干生物量显著增加,表明种子催种对幼苗首次生长也有潜在影响。本研究结果表明,种子催熟对千叶种子萌发、出苗和早期生长有显著的正向影响。
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引用次数: 1
Machine vision detection of pests, diseases, and weeds: A review 害虫、疾病和杂草的机器视觉检测:综述
Pub Date : 2020-04-17 DOI: 10.25081/jp.2020.v12.6145
Chiranjeevi Muppala, Velmathi Guruviah
Most of mankind’s living and workspace have been or going to be blended with smart technologies like the Internet of Things. The industrial domain has embraced automation technology, but agriculture automation is still in its infancy since the espousal has high investment costs and little commercialization of innovative technologies due to reliability issues. Machine vision is a potential technique for surveillance of crop health which can pinpoint the geolocation of crop stress in the field. Early statistics on crop health can hasten prevention strategies such as pesticide, fungicide applications to reduce the pollution impact on water, soil, and air ecosystems. This paper condenses the proposed machine vision relate research literature in agriculture to date to explore various pests, diseases, and weeds detection mechanisms.
人类的大部分生活和工作空间已经或即将与物联网等智能技术融合在一起。工业领域已经接受了自动化技术,但农业自动化仍处于起步阶段,因为其投入成本高,而且由于可靠性问题,创新技术的商业化很少。机器视觉是一种有潜力的作物健康监测技术,它可以精确定位田间作物胁迫的地理位置。作物健康的早期统计数据可以加快采取预防策略,如农药、杀菌剂的应用,以减少污染对水、土壤和空气生态系统的影响。本文总结了迄今为止机器视觉在农业领域的相关研究文献,探讨了各种病虫害和杂草的检测机制。
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引用次数: 7
Phytochemical analysis and antioxidant activity of Haloxylon salicarnicum, Ochradenus arabicus and Tamarix nilotica 含水梭梭、阿拉伯梭梭和柽柳的植物化学分析及其抗氧化活性
Pub Date : 2019-12-31 DOI: 10.25081/jp.2019.v11.6149
M. Alshamsi, Alghazal K. H. Alnuaimi, A. Senthilkumar, R. Alshamsi, K. Karthishwaran, Wasef Al-Zayadneh, A. Cheruth
In the present investigation, the preliminary phytochemical analysis and antioxidant potential of hexane, chloroform, acetone and methanol extracts of Haloxylon salicarnicum, Ochradenus arabicus and Tamarix nilotica were reported. The presence of flavonoid, alkaloid, phenols, terpenoids, carbohydrate, tannin, protein, steroids, saponin, phlobatannin, cardiacglycoside, anthraquinines and volatile oils were determined and for antioxidant activity, DPPH, ABTS, superoxide anion radical and hydroxyl radical scavenging potential were studied. Strong presence of alkaloid, tannin, steroids in H. salicarnicum, flavonoid and phenols in O. arabicus and saponin T. nilotica were observed. In the present investigation, all the extracts inhibited the free radicals the methanol extract of O. arabicus was identified as potential crude extract compared to all other extracts with the IC50 values of 91.65 (DPPH), 94.62 (ABTS), 95.82 (O2) and 96.02 (OH) μg/mL. Whereas, the IC50 value of the standard, Gallic acid were 125.25 (DPPH), 142.32 (ABTS), 130.78 (O2) and 139.93 μg/mL (OH). Whereas, the IC50 value of the standard, Gallic acid were 125.25 (DPPH), 142.32 (ABTS), 130.78 (O2) and 139.93 μg/mL (OH)The present study suggests that methanol extract of O. arabicus can be used for the isolation potential natural antioxidant.
本文报道了盐梭梭(Haloxylon salicarnicum)、阿拉伯蛇柳(Ochradenus arabicus)和柽柳(Tamarix nilotica)的己烷、氯仿、丙酮和甲醇提取物的初步植物化学分析和抗氧化活性。测定了黄酮类、生物碱、酚类、萜类、碳水化合物、单宁、蛋白质、甾体、皂素、酞菁、心糖苷、蒽醌和挥发油的含量,并对其抗氧化活性、DPPH、ABTS、超氧阴离子自由基和羟基自由基的清除能力进行了研究。水杨中含有大量的生物碱、单宁和甾体,阿拉伯藜中含有大量的类黄酮和酚类物质,尼罗河草中含有大量的皂苷。在本研究中,所有提取物均具有抑制自由基的作用,其中甲醇提取物的IC50值分别为91.65 (DPPH)、94.62 (ABTS)、95.82 (O2)和96.02 (OH) μg/mL。标准品没食子酸的IC50值分别为125.25 (DPPH)、142.32 (ABTS)、130.78 (O2)和139.93 μg/mL (OH)。而标准品没食子酸的IC50值分别为125.25 (DPPH)、142.32 (ABTS)、130.78 (O2)和139.93 μg/mL (OH)。本研究表明,阿拉伯草甲醇提取物可作为潜在的天然抗氧化剂进行分离。
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引用次数: 4
EFFECT OF ENRICHED SANJEEVANI AND AGNIHOTRA ON GROWTH, YIELD AND QUALITY OF BRINJAL (Solanum melongena L.) 富余桑吉瓦尼和AGNIHOTRA对茄子生长、产量和品质的影响
Pub Date : 2019-12-31 DOI: 10.25081/jp.2019.v11.3818
S. Tripathy, A. Dutta
Brinjal is a crop grown widely all over India and preferred by both rich and poor. The Chhotanagpur plateau of Jharkhand is famous for quality vegetable production and brinjal is very commonly grown in this region almost throughout the year. However, the most of the commercial growers using plant protection chemicals and synthetic fertilizers those are so expensive that poor farmers can’t afford. The extensive uses of agro-chemicals and synthetic fertilizers also reduce the quality of both the produce and the cultivated soil. In this context, an attempt has been made through the present investigation by growing different varieties of brinjal by adopting Vedic (Enriched Sanjeevani) Farming and Homa Induction (Agnihotra) techniques with their respective four non-chemical alternative growing approaches, viz. E1C1: Enriched Sanjeevani (1%), E1C2: Absolute Control (inherent fertility status of the experimental plot); E2C1: Enriched Sanjeevani (1%) + Homa Induction (Agnihotra), and E2C2: Only Homa Induction (Agnihotra). Five varieties of the crop, viz. V1: Swarna Pratibha; V2: Swarna Neelima; V3: Swarna Shakti; V4: Mukta Jhuri; V5: Long Green were grown with their four replications under four growing conditions and the Randomized Completed Block Design was adopted for field trials. Different growth, yield and quality attributing characters of the crop were taken into account and findings revealed that E2C1: Enriched Sanjeevani (1%) + Homa Induction (Agnihotra) was the most suitable growing condition for V2 (Swarna Neelima) with the maximum yield (72.37 t ha -1). Different growth and yield attributes of the crop varieties (especially in the case of V2: Swarna Neelima) were highly influenced by Enriched Sanjeevani (1%) + Homa Induction (Agnihotra) [E2C1] growing condition resulting higher benefit cost ratio of 6.78. Quality contributing attributes were also highly influenced by homa induction (Agnihotra), as a consequence, higher level of dry matter, TSS, and ascorbic acid contents were estimated from almost all samples collected from E2C1: Enriched Sanjeevani (1%) + Homa Induction (Agnihotra), and E2C2: Only Homa Induction (Agnihotra) growing conditions.
茄子是一种在印度广泛种植的作物,富人和穷人都喜欢它。贾坎德邦的乔塔纳格布尔高原以优质蔬菜生产而闻名,该地区几乎全年都在种植茄子。然而,大多数商业种植者使用植物保护化学品和合成肥料,这些都太贵了,贫穷的农民负担不起。农用化学品和合成肥料的广泛使用也降低了农产品和耕地的质量。在此背景下,通过本研究,通过采用Vedic(浓缩Sanjeevani)耕作和Homa诱导(Agnihotra)技术种植不同品种的茄子,分别采用四种非化学替代种植方法,即E1C1:浓缩Sanjeevani (1%), E1C2:绝对控制(试验田的固有生育状况);E2C1:富含Sanjeevani (1%) + Homa诱导(Agnihotra), E2C2:仅Homa诱导(Agnihotra)。五个品种的作物,即V1: Swarna Pratibha;V2: Swarna Neelima;V3: Swarna Shakti;V4: Mukta Jhuri;V5:在4种生长条件下,分4个重复种植长绿,田间试验采用随机完全区设计。综合考虑不同作物的生长、产量和品质属性,结果表明,E2C1:富集Sanjeevani (1%) + Homa诱导(Agnihotra)是V2 (Swarna Neelima)最适宜的生长条件,产量最高(72.37 tha -1)。丰化Sanjeevani (1%) + Agnihotra (E2C1)生长条件对作物品种(特别是V2: Swarna Neelima)的生长和产量属性影响较大,其效益成本比高达6.78。质量贡献属性也受到homa诱导(Agnihotra)的高度影响,因此,在E2C1:富集Sanjeevani (1%) + homa诱导(Agnihotra)和E2C2:仅homa诱导(Agnihotra)生长条件下收集的几乎所有样品中,估计干物质、TSS和抗坏血酸含量较高。
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引用次数: 2
Induced mutagenesis in Cicer arietinum by the application of EMS and Gamma rays with special reference to the cytological studies 应用电磁辐射和伽玛射线诱导突变,并特别参考细胞学研究
Pub Date : 2018-05-07 DOI: 10.25081/JP.2018.V10.3455
S. Umavathi, L. M. I. Anittha
The present study was conducted to analyze the chemical and physical mutagenesis on Cicer arietinum with special reference to cytological studies. In this regard, CO–4 variety of chick pea was subjected to different concentration of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50 mmol) for inducing mutation. The M1 plants exposed to mutagen produces a clear cut difference from the untreated control. The root mitotic studies reveal a wide range of chromosomal aberrations such as stickiness, laggards, bridges and some other precocious movement. The percentage of abnormal cell increased with dosage in both mutagens; and 50 mmol EMS showed more chromosomal aberrations when compared to gamma rays.
本研究以细胞学研究为基础,分析了黄颡鱼的化学诱变和物理诱变。在不同浓度的γ射线(20、30、40、50和60kR)和EMS(10、20、30、40和50 mmol)胁迫下,CO-4鹰嘴豆品种诱导突变。暴露于诱变剂的M1植株与未经处理的对照产生明显的差异。根有丝分裂研究揭示了广泛的染色体畸变,如黏性、迟滞性、桥性和其他一些早熟运动。两种诱变剂的异常细胞百分比均随剂量增加而增加;与伽马射线相比,50 mmol EMS显示出更多的染色体畸变。
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引用次数: 0
A SHEA BUTTER RICH IN TOCOPHEROLS (VITAMIN E) AT THE DOGON PLATEAU AND SENO BANKASS IN MALI (WEST AFRICA) 西非马里的多冈高原和seno bankass富含生育酚(维生素e)的乳木果油。
Pub Date : 2018-05-07 DOI: 10.25081/JP.2018.V10.3743
B. Kelly, F. Davrieux, J. Bouvet
Vitellaria paradoxa, a forest tree species plays an important role for rural populations in Mali. The kernel is rich in fat, fatty acids and tocopherols and the butter extrated from the kernel is used in many African kitchens, in pharmacology, cosmetics, local traditional medicine and as Chocolate Butter Equivalent (CBE) in chocolate industry. A consortium funded by the Europena Union has worked on several aspects of shea tree including chemical characterisation of shea butter. In Mali, one of the project partners, five sites were selected, fruits were collected from selected shea trees and sent to Montpellier for chemical analyses assessing among other variables the tocopherol content of the shea butter using liquid chromatography (HPLC). Results of this study shown that shea butter from the Dogon Plateau and the Seno Bankass is richer in tocopherols (Vitamin E) compared to other study sites. Sites were found significantly different for tocopherols content all together as well as for each type of tocopherol. The richness of shea butter from the Dogon Plateau and the Seno Bankass in tocopherols confers to it an important nutritional value for the good healf of rural populations of this zone, shea butter being the main source of fat for cooking in this area.
在马里,森林树种Vitellaria paradoxa对农村人口起着重要作用。核桃仁富含脂肪、脂肪酸和生育酚,从核桃仁中提取的黄油被用于许多非洲人的厨房、药理学、化妆品、当地传统医药和巧克力工业中的巧克力黄油当量(CBE)。由欧盟资助的一个财团对乳木果树的几个方面进行了研究,包括乳木果油的化学特性。在项目合作伙伴之一的马里,选定了五个地点,从选定的乳木果树上采集果实,并送往蒙彼利埃进行化学分析,使用液相色谱法(HPLC)评估乳木果油的生育酚含量等变量。本研究结果表明,与其他研究地点相比,多冈高原和Seno Bankass的乳木果油含有更丰富的生育酚(维生素E)。研究发现,不同部位的生育酚含量以及不同类型的生育酚含量都有显著差异。来自多贡高原和Seno Bankass的丰富的乳木果油的生育酚赋予了它对该地区农村人口健康的重要营养价值,乳木果油是该地区烹饪脂肪的主要来源。
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引用次数: 1
期刊
The Journal of Phytology
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