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One step pre-hardening micropropagation of Bambusa balcooa Roxb. 竹竹一步预硬化微繁技术研究。
Pub Date : 2015-11-10 DOI: 10.5455/JP.2015-06-02
B. Patel, B. Gami, N. Patel, Vipul Bariya.
Bamboo’s fast growth and ability to sequester atmospheric carbon consequently mitigate climate change.Abellon’s mission is to reduce CO2 from the environment by growing bamboo on marginal land and using this biomass for bioenergy. Bambusa balcooa Roxb. micropropagation protocol is established for a consistent supply of quality plantlets. Surface sterilization of nodal explants using 0.1% mercuric chloride followed by initiation in liquid and solid media with and without Gentamycin (3.0-8.0 mg/L) containing Murashige and Skoog (MS) media supplemented with 0.01% myo-inositol, 3% sugar, 25 mg/L citrate 50 mg/L ascorbate, and 3.5 mg/L 6-benzylaminopurine (BAP) as growth regulator. Shoots were multiplied using MS medium augmented with 3% sugar, 6% agar, 0.01% myo-inositol with 3 mg/L BAP and 0.5 mg/L naphthalene acetic acid (NAA). Shoot clusters were rooted in MS supplemented with 4 mg/L NAA, pre-hardened in half MS, acclimatized using coco peat, and vermicompost while at net house using vermicompost, soil, sand, and vesicular arbuscular mycorrhiza (VAM) culture. 97% bud break was achieved from initiation while overall success ratio for establishment was 85%. Sub-culturing shoots showed proliferation rate of 3.5-fold. Rooting was successfully achieved with 83% rate.Acclimatization rate at primary and secondary hardening was 72%. The uniqueness of the study lies as bamboo confines to tropical region, however; we have described B. balcooa mass multiplication protocol using explants from arid region with one step pre-hardening process. The protocol achieved multiplication rate of 3.5-fold, overall survival rate 74.66% using vermicompost and VAM for acclimatization of B. balcooa.
竹子的快速生长和封存大气碳的能力,从而减缓了气候变化。Abellon的任务是通过在边缘土地上种植竹子并利用这种生物质作为生物能源来减少环境中的二氧化碳。竹阳台。为保证优质植株的持续供应,建立了微繁协议。在含庆大霉素(3.0-8.0 mg/L)的液体和固体培养基中进行表面灭菌,然后在含有0.01%肌醇、3%糖、25 mg/L柠檬酸、50 mg/L抗坏血酸和3.5 mg/L 6-氨基嘌呤(BAP)作为生长调节剂的Murashige和Skoog (MS)培养基中初始化。培养基中添加3%糖、6%琼脂、0.01%肌醇、3mg /L BAP和0.5 mg/L萘乙酸(NAA)。茎簇在添加4 mg/L NAA的MS中生根,在一半MS中预硬化,用椰子泥炭和蚯蚓堆肥驯化,在网房用蚯蚓堆肥、土壤、沙子和泡状丛枝菌根(VAM)培养。发芽率为97%,建立总成功率为85%。继代苗的增殖率为3.5倍。生根成功率为83%。初次和二次硬化的驯化率为72%。然而,这项研究的独特之处在于竹子仅限于热带地区;本文描述了利用干旱区外植体采用一步预硬化法进行巴氏白僵菌大规模增殖的方法。采用蚯蚓堆肥和VAM对balcoa进行驯化,繁殖率达到3.5倍,总存活率为74.66%。
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引用次数: 13
Distribution pattern of freshwater cyanobacteria in Kaiga region of Western Ghats of Karnataka 卡纳塔克邦西高止山脉Kaiga地区淡水蓝藻分布格局
Pub Date : 2015-11-10 DOI: 10.5455/JP-2015-09-017
K. Chandra, M. Rajashekhar
This study deals with the identification of 59 cyanobacterial species belonging to 27 genera from different freshwater habitats of Kaiga in Uttara Kannada district of Karnataka during the period from June 2009 to May 2011. Samplings were made during pre-monsoon; monsoon and post-monsoon sampling have been carried out for the duration of 2 years. The study deals with the occurrence of cyanobacterial species in five different aquatic habitats in Kaiga region, with respect to a change in the physico-chemical properties of water. The cyanobacterial diversity is maximum in monsoon season compared to post-monsoon; it was least during pre-monsoon. Among cyanobacteria non-heterocystous filamentous forms were dominant followed by unicellular forms, whereas heterocystous forms were least in number. It was also found that the physicho-chemical properties of water have the influence on the richness of cyanobacterial community. This study indicates the maximum occurrence and abundance of Chroococaceae (23.73%) and Phormidaceae (18.64%) members in all the sites, whereas Stigonemataceae (1.7%) shows very less occurrence. Among the cyanobacteria identified non-heterocystous filamentous forms were dominant followed by unicellular forms; whereas heterocystous forms were least in number.
本研究对2009年6月至2011年5月在卡纳塔克邦北坎纳达地区不同淡水生境的59种蓝藻进行了鉴定,隶属于27属。取样是在季风前进行的;季风及季风后取样已进行了两年。本研究研究了海加地区五种不同水生生境中蓝藻物种的发生,以及水的物理化学性质的变化。与季风期相比,季风期蓝藻多样性最大;季风前最少。在蓝藻中,非异囊丝状形式占主导地位,其次是单细胞形式,而异囊形式的数量最少。水体的理化性质对蓝藻群落的丰富度也有影响。研究结果表明,在所有站点中,chroocoaceae(23.73%)和Phormidaceae(18.64%)成员的发生率和丰度最高,而Stigonemataceae(1.7%)成员的发生率极低。在所鉴定的蓝藻中,非异囊丝状菌占主导地位,其次是单细胞菌;而异囊型的数量最少。
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引用次数: 0
Influence of long-term use of organic and inorganic nutrients on HLB disease of wheat under rice-wheat cropping pattern. 长期施用有机和无机养分对稻麦种植模式下小麦HLB病的影响
Pub Date : 2010-10-20 DOI: 10.3126/NARJ.V9I0.11644
D. Bhandari
SUMMARY Helminthosporium leaf blight (HLB) is one of the most destructive diseases of wheat in terai (plain) region of Nepal. This study was conducted to determine the effect of long-term application of various levels of Nitrogen (N), Phosphorus (P), Potassium (K) and organic nutrients on HLB disease of wheat. The experiment was super imposed on long-term fertility experiment conducted in RCB design with three replications under rice- wheat cropping pattern at National wheat research program (NWRP), Bhairahawa, Nepal. The treatments include various combinations of 100 kg Nitrogen/ha, 0 and 60 kg Phosphorus/ha and 0, 50 and 100 kg Potash /ha with or without Sesbania ( Sesbania cannabina ) and Farm Yard Manure (FYM). Area under disease progress curve was calculated using the disease scores and yield parameters were recorded. Among the inorganic nutrients, long-term deficiency of potash in the field markedly enhances the HLB disease. Application of phosphorus in various doses increased the HLB severity in absence of potash. Regular application of at least 50 kg potash/ha in wheat noticeably reduced the HLB disease. Regular use of FYM 10 ton/ha for long term considerably reduced the HLB disease but long term use of Sesbania as green manure hadn’t any impact on HLB severity. Inclusion of potash in nutrients considerably increased the yield and thousand-grain weight (TGW) of wheat. In long term, balanced use of inorganic and organic nutrients with special attention on regular inclusion of potash and FYM in the nutrient combinations is vital in suppression of HLB disease under rice-wheat cropping pattern. Key words: AUDPC, FYM, Helminthosporium leaf blight, Phosphorus, Potassium, Sesbania Deepak Bhandari. Influence of Long-term Use of Organic and Inorganic Nutrients on HLB Disease of Wheat under Rice-wheat Cropping Pattern. J Phytol 2/2 (2010) 73-79.
摘要Helminthosporium leaf blight (HLB)是尼泊尔平原地区小麦最具破坏性的病害之一。本试验旨在研究长期施用不同水平氮、磷、钾和有机养分对小麦HLB病的影响。本试验是在尼泊尔Bhairahawa国家小麦研究计划(NWRP)水稻-小麦种植模式下按RCB设计进行的3个重复长期肥力试验的基础上进行的。处理包括100公斤氮/公顷、0和60公斤磷/公顷以及0、50和100公斤钾肥/公顷的不同组合,加或不加田葵(大麻田葵)和农场厩肥(FYM)。利用病害评分计算病害进展曲线下面积,记录产量参数。在无机养分中,长期缺乏钾肥对HLB病有显著的促进作用。在没有钾肥的情况下,施用不同剂量的磷增加了HLB的严重程度。小麦定期施用钾肥至少50公斤/公顷,可显著减少HLB病。长期使用10吨/公顷的禾草肥可显著减少HLB病,但长期使用田菁作为绿肥对HLB病的严重程度没有影响。在养分中掺入钾肥可显著提高小麦的产量和千粒重。从长期来看,平衡使用无机和有机营养物,特别注意在营养组合中定期添加钾肥和FYM,对于抑制水稻-小麦种植模式下的HLB病至关重要。关键词:AUDPC, FYM, Helminthosporium leaf疫病,磷,钾,田葵长期施用有机和无机养分对稻麦种植模式下小麦HLB病的影响[J] .植物生理学报(2010):73-79。
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引用次数: 3
Root morphology and root characters of different cocoa (Theobroma cacao L) types at 100 and 50 percent field capacity under water deficit condition 水分亏缺条件下不同可可(Theobroma cacao L)品种在100%和50%田间容量下的根系形态和根系性状
Pub Date : 1970-01-01 DOI: 10.25081/JP.2019.V11.3786
V. Jegadeeswari, K. Arunkumar, B. G. B. Santhini
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引用次数: 1
Mutagenic effect of EMS and DES on Tenai (Setaria italica) in M1 generation EMS和DES对意大利雪雀花M1代的诱变效应
Pub Date : 1970-01-01 DOI: 10.25081/JP.2018.V10.3406
I. Anittha, L. Mullainathan
Mutation breeding gives better results for crop improvement through genetic manipulations when compared to other conventional breeding techniques. The present work focused in order to find out the effect of chemical mutagens; EMS and DES on Setaria italica in M1 generation. The seeds of Tenai, variety CO(Te)7 treated with different concentration of EMS and DES. The LD50 was observed at 30 mmol in EMS and 40 mmol in DES. Selection studies were conducted to improve the yield and to generate genetic variability in different quantitative traits such as days to first bloom, plant height, number of leaves, number of nodes, length and breadth of ear head, 1000 seed weight and yield per plant. The results revealed that, all the parameters were decreased with increasing concentration in both EMS and DES, while days to first bloom was increasing with increasing concentration. According to the result all the parameters studied shows a negative direction towards crop improvement in M1 generation because of the stress caused by mutagenic treatment.
与其他传统育种技术相比,突变育种通过遗传操作为作物改良提供了更好的结果。目前的工作重点是为了找出化学诱变剂的作用;M1代意大利狗尾草的EMS和DES研究。用不同浓度的EMS和DES处理Tenai品种CO(Te)7的种子,EMS处理的LD50为30 mmol, DES处理的LD50为40 mmol。通过选择研究提高产量,并在开花日数、株高、叶数、节数、穗长、穗宽、千粒重和单株产量等不同数量性状上产生遗传变异。结果表明,EMS和DES均随浓度的增加而降低,而开花天数随浓度的增加而增加。结果表明,诱变处理造成的胁迫对M1代作物改良有不利影响。
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引用次数: 1
Variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India 印度拉贾斯坦邦不同地区藤果营养价值的差异
Pub Date : 1970-01-01 DOI: 10.25081/JP.2018.V10.3420
Mala Rathore
In the present investigation, an attempt has been made to identify the variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India. Fruits were collected from Jodhpur (Kailana, Bilara) and Barmer (Siwana) region and analyzed nutritionally. Maximum ash and vitamin C content was obtained in samples from Siwana (4.73% and 56.47% respectively). Maximum fat content (2.66 %) was obtained in samples from Bilara. Maximum sugar and protein content was obtained in samples from Kailana (35.51% and 8.4% respectively). They are rich in vitamin C (39 mg/100 gm) content. The fruits are also rich sources of minerals such as Ca (0.32±0.03 g/100g), K (1.26±0.31g/100 g) and Na (1.49±0.32 g/100g) than the cultivated crop plants. Trace elements such as Fe, Zn, Cu, Mn and Mg were also analyzed. These results of nutritional composition were compared with those collected from Bilara and Siwana area of Jodhpur region. Many medicinal applications of G. tenax viz. as remedy for colds and chest complaints have also been reported. Thus, the importance of G. tenax in the rural economy is highly significant.
在目前的调查中,试图确定来自印度拉贾斯坦邦不同地区的藤果营养价值的差异。水果采集自焦特布尔(Kailana, Bilara)和巴默(Siwana)地区,并进行营养分析。西瓦纳的灰分和维生素C含量最高,分别为4.73%和56.47%。比拉拉的脂肪含量最高,为2.66%。糖和蛋白质含量最高,分别为35.51%和8.4%。它们含有丰富的维生素C(39毫克/100克)。与栽培作物相比,水果含有丰富的钙(0.32±0.03 g/100g)、钾(1.26±0.31g/ 100g)和钠(1.49±0.32 g/100g)等矿物质。同时对铁、锌、铜、锰、镁等微量元素进行了分析。这些营养成分结果与焦特布尔地区Bilara和Siwana地区收集的结果进行了比较。许多药用用途,即治疗感冒和胸部疾病也有报道。因此,黄芪在农村经济中的重要性是非常显著的。
{"title":"Variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India","authors":"Mala Rathore","doi":"10.25081/JP.2018.V10.3420","DOIUrl":"https://doi.org/10.25081/JP.2018.V10.3420","url":null,"abstract":"In the present investigation, an attempt has been made to identify the variation in nutritional value of Grewia tenax fruits from different regions of Rajasthan, India. Fruits were collected from Jodhpur (Kailana, Bilara) and Barmer (Siwana) region and analyzed nutritionally. Maximum ash and vitamin C content was obtained in samples from Siwana (4.73% and 56.47% respectively). Maximum fat content (2.66 %) was obtained in samples from Bilara. Maximum sugar and protein content was obtained in samples from Kailana (35.51% and 8.4% respectively). They are rich in vitamin C (39 mg/100 gm) content. The fruits are also rich sources of minerals such as Ca (0.32±0.03 g/100g), K (1.26±0.31g/100 g) and Na (1.49±0.32 g/100g) than the cultivated crop plants. Trace elements such as Fe, Zn, Cu, Mn and Mg were also analyzed. These results of nutritional composition were compared with those collected from Bilara and Siwana area of Jodhpur region. Many medicinal applications of G. tenax viz. as remedy for colds and chest complaints have also been reported. Thus, the importance of G. tenax in the rural economy is highly significant.","PeriodicalId":22829,"journal":{"name":"The Journal of Phytology","volume":"35 1","pages":"12-14"},"PeriodicalIF":0.0,"publicationDate":"1970-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72965837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Application of machine learning in detection of blast disease in South Indian rice crops 机器学习在南印度水稻稻瘟病检测中的应用
Pub Date : 1970-01-01 DOI: 10.25081/JP.2019.V11.5476
S. Ramesh, D. Vydeki
It is a well-known fact that the quality and quantity of the rice crop is reduced due to plant disease. This paper proposes rice blast disease detection mechanism using Machine learning algorithm, to identify the disease in the early stage of the crop cultivation. The proposed method would find the blast disease and reduce the crop loss and hence increase the rice agriculture production in an effective manner. The images of the paddy field are captured and eight features are extracted to distinguish the healthy and the disease affected leaves. The proposed machine learning based classification methodology includes KNN and ANN. The performance of these two classification techniques is compared using an appropriate confusion matrix. The simulation results show that KNN based classification method provides an accuracy of 85% for the blast affected leaf images and 86% for the normal leaf images. The accuracy is improved to 99% and 100% respectively for the ANN based classification mechanisms.
众所周知,由于植物病害,水稻作物的质量和数量都下降了。本文提出了利用机器学习算法的稻瘟病检测机制,在作物栽培的早期阶段识别病害。该方法可以有效地发现稻瘟病,减少作物损失,从而提高水稻农业产量。采集稻田图像,提取8个特征来区分健康叶片和病害叶片。提出的基于机器学习的分类方法包括KNN和ANN。使用适当的混淆矩阵比较了这两种分类技术的性能。仿真结果表明,基于KNN的分类方法对爆炸影响叶片图像的分类准确率为85%,对正常叶片图像的分类准确率为86%。对于基于人工神经网络的分类机制,准确率分别提高到99%和100%。
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引用次数: 34
Organic nutrient management technique for enhancing growth and physiological parameters in radish (Raphanus sativus L.) 促进萝卜生长和生理参数的有机养分管理技术
Pub Date : 1970-01-01 DOI: 10.25081/JP.2018.V10.3461
A. Mani, A. Anburani
Among the various technologies that boost the production of crop, nutrient management has got an important impact in maximizing the yield. Application of balanced fertilizers alone is not only the solution for this problem, application of residue free nutrients that protects the soil health and environment is very important. Here comes the concept of organic farming. Hence, a field experiment was conducted in the Department of Horticulture, Faculty of Agriculture, Annamalai University, Annamalai Nagar to study the effect of organic nutrients on growth and physiological parameters of radish. The experiment was conducted with eighteen treatments in three replications and laid out in FRBD design. The treatment includes various sources of organic manures viz., FYM, vermicompost, enriched manure along with and without use of consortium biofertilizer. Biostimulants viz., buttermilk solution and EMI were given as foliar spray at 15 d interval as 2 sprays. The intercultural operations and plant protection measures were carried out as per the recommendation. The results of the experiment revealed that application of farm yard manure @ 25 t ha-1 along with consortium biofertilizer @ 2 Kg ha-1 and EMI @ 1:1000 dilution ha1 as foliar application recorded the highest growth parameters viz., shoot length, number of leaves and shoot weight. The physiological parameters viz., leaf area and chlorophyll content were recorded the highest in the same treatment T6.
在促进作物生产的各种技术中,养分管理对产量最大化具有重要影响。仅施用平衡肥并不能解决这一问题,施用无残留养分对保护土壤健康和环境至关重要。这就是有机农业的概念。为此,在安纳玛莱大学农学院园艺系进行了田间试验,研究有机养分对萝卜生长和生理参数的影响。试验采用FRBD设计,分3个重复,共18个处理。该处理包括各种来源的有机肥料,即FYM,蚯蚓堆肥,强化肥料以及使用或不使用联合生物肥料。生物刺激剂,即酪乳溶液和EMI作为叶面喷雾,每隔15 d喷2次。跨文化行动和植物保护措施是按照建议进行的。试验结果显示,叶面施用农家肥25 t hm -1、联合生物肥料2 Kg hm -1和EMI 1:1000稀释hm -1,记录了最高的生长参数,即茎长、叶片数量和茎重。同一处理T6的叶面积、叶绿素含量等生理指标最高。
{"title":"Organic nutrient management technique for enhancing growth and physiological parameters in radish (Raphanus sativus L.)","authors":"A. Mani, A. Anburani","doi":"10.25081/JP.2018.V10.3461","DOIUrl":"https://doi.org/10.25081/JP.2018.V10.3461","url":null,"abstract":"Among the various technologies that boost the production of crop, nutrient management has got an important impact in maximizing the yield. Application of balanced fertilizers alone is not only the solution for this problem, application of residue free nutrients that protects the soil health and environment is very important. Here comes the concept of organic farming. Hence, a field experiment was conducted in the Department of Horticulture, Faculty of Agriculture, Annamalai University, Annamalai Nagar to study the effect of organic nutrients on growth and physiological parameters of radish. The experiment was conducted with eighteen treatments in three replications and laid out in FRBD design. The treatment includes various sources of organic manures viz., FYM, vermicompost, enriched manure along with and without use of consortium biofertilizer. Biostimulants viz., buttermilk solution and EMI were given as foliar spray at 15 d interval as 2 sprays. The intercultural operations and plant protection measures were carried out as per the recommendation. The results of the experiment revealed that application of farm yard manure @ 25 t ha-1 along with consortium biofertilizer @ 2 Kg ha-1 and EMI @ 1:1000 dilution ha1 as foliar application recorded the highest growth parameters viz., shoot length, number of leaves and shoot weight. The physiological parameters viz., leaf area and chlorophyll content were recorded the highest in the same treatment T6.","PeriodicalId":22829,"journal":{"name":"The Journal of Phytology","volume":"53 1","pages":"40-42"},"PeriodicalIF":0.0,"publicationDate":"1970-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82280286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Seed priming technology in spice crops: A review 香料作物种子引种技术研究进展
Pub Date : 1970-01-01 DOI: 10.25081/JP.2019.V11.3787
K. Arunkumar, V. Jegadeeswari, C. Ushamalini
{"title":"Seed priming technology in spice crops: A review","authors":"K. Arunkumar, V. Jegadeeswari, C. Ushamalini","doi":"10.25081/JP.2019.V11.3787","DOIUrl":"https://doi.org/10.25081/JP.2019.V11.3787","url":null,"abstract":"","PeriodicalId":22829,"journal":{"name":"The Journal of Phytology","volume":"134 1","pages":"21-24"},"PeriodicalIF":0.0,"publicationDate":"1970-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87704187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
EFFECT OF MUTAGENS ON QUANTITATIVE CHARACTERS IN M3 GENERATION OF PEARL MILLET (PENNISETUM TYPHOIDES (BURN.F) STAPF. AND C.E. HUBB.) 诱变剂对珍珠粟m3代数量性状的影响[j]。还有c.e.哈伯。)
Pub Date : 1970-01-01 DOI: 10.25081/JP.2018.V10.3404
K. Ambli, L. Mullainathan
{"title":"EFFECT OF MUTAGENS ON QUANTITATIVE CHARACTERS IN M3 GENERATION OF PEARL MILLET (PENNISETUM TYPHOIDES (BURN.F) STAPF. AND C.E. HUBB.)","authors":"K. Ambli, L. Mullainathan","doi":"10.25081/JP.2018.V10.3404","DOIUrl":"https://doi.org/10.25081/JP.2018.V10.3404","url":null,"abstract":"","PeriodicalId":22829,"journal":{"name":"The Journal of Phytology","volume":"31 1","pages":"01-05"},"PeriodicalIF":0.0,"publicationDate":"1970-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79836064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
The Journal of Phytology
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