Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0137:CSOLD>2.0.CO;2
K. A. Anderson, P. Rosenblum, B. Whiteside, R. W. Standage, T. Brandt
Abstract Temperature (5–25°C) and photoperiod (daylight of 10–14 h) were manipulated to induce spawning in groups and individual pairs of longnose darters Percina nasuta. The fish were maintained in tanks for up to 6 years and were fed blackworms, zooplankton, and aquatic insects harvested from hatchery ponds. The fish were spawned four times between June 1994 and February 1996. Eight females released over 3,400 eggs, of which 73% were deposited on Spawntex mats. Various incubation methods were used, and 706 fry (20% hatch) were produced. Fry were offered brine shrimp Artemia sp., copepods, and cladocerans (0.053–0.212 mm in diameter) and rotifers (0.053–0.106 mm in diameter) under various culture conditions. Mortality of fry was 100% within 9 d.
{"title":"Controlled Spawning of Longnose Darters","authors":"K. A. Anderson, P. Rosenblum, B. Whiteside, R. W. Standage, T. Brandt","doi":"10.1577/1548-8640(1998)060<0137:CSOLD>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0137:CSOLD>2.0.CO;2","url":null,"abstract":"Abstract Temperature (5–25°C) and photoperiod (daylight of 10–14 h) were manipulated to induce spawning in groups and individual pairs of longnose darters Percina nasuta. The fish were maintained in tanks for up to 6 years and were fed blackworms, zooplankton, and aquatic insects harvested from hatchery ponds. The fish were spawned four times between June 1994 and February 1996. Eight females released over 3,400 eggs, of which 73% were deposited on Spawntex mats. Various incubation methods were used, and 706 fry (20% hatch) were produced. Fry were offered brine shrimp Artemia sp., copepods, and cladocerans (0.053–0.212 mm in diameter) and rotifers (0.053–0.106 mm in diameter) under various culture conditions. Mortality of fry was 100% within 9 d.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"60 1","pages":"137-145"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83959265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0088:COFAER>2.0.CO;2
L. Ford, P. Barbash, R. Cipriano
Abstract Adult sea-run Atlantic salmon Salmo salar captured and transported to Richard Cronin National Salmon Station (Sunderland, Massachusetts), Nashua National Fish Hatchery (Nashua, New Hampshire), and Whittemore State Fish Hatchery (Waterford, Connecticut) during 1986–1992 were treated with oxolinic acid and a bacterin. The bacterin was developed against furunculosis and enteric redmouth disease. Among the 2,552 fish that were treated since 1986, 362 died and 65 (18%) of those fish had furunculosis. Among 206 untreated fish that were maintained as controls, 109 died and 63 (57.8%) had furunculosis. The reduction in mortality could not be attributed to either vaccine or antibiotic alone without further study. A 3-year study was designed to investigate if adult Atlantic salmon, undergoing the stress of migration, handling, and spawning, could mount a protective humoral immune response. Although the salmon were able to produce an agglutinin response, evidence was not found for production of a protective...
{"title":"Control of Furunculosis and Enteric Redmouth Disease in Sea‐Run Atlantic Salmon Broodstock in the Connecticut and Merrimack Rivers","authors":"L. Ford, P. Barbash, R. Cipriano","doi":"10.1577/1548-8640(1998)060<0088:COFAER>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0088:COFAER>2.0.CO;2","url":null,"abstract":"Abstract Adult sea-run Atlantic salmon Salmo salar captured and transported to Richard Cronin National Salmon Station (Sunderland, Massachusetts), Nashua National Fish Hatchery (Nashua, New Hampshire), and Whittemore State Fish Hatchery (Waterford, Connecticut) during 1986–1992 were treated with oxolinic acid and a bacterin. The bacterin was developed against furunculosis and enteric redmouth disease. Among the 2,552 fish that were treated since 1986, 362 died and 65 (18%) of those fish had furunculosis. Among 206 untreated fish that were maintained as controls, 109 died and 63 (57.8%) had furunculosis. The reduction in mortality could not be attributed to either vaccine or antibiotic alone without further study. A 3-year study was designed to investigate if adult Atlantic salmon, undergoing the stress of migration, handling, and spawning, could mount a protective humoral immune response. Although the salmon were able to produce an agglutinin response, evidence was not found for production of a protective...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"16 15 1","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87078892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0127:CVRFRO>2.0.CO;2
E. Goolish, R. Evans, K. Okutake, Rachael Max
Abstract The zebrafish or zebra danio Danio (=Brachydanio) rerio has recently become a major vertebrate model for the study of developmental biology, neurobiology, and molecular genetics. As a result, most research universities have now invested considerable resources in the construction of large zebrafish facilities. A key element in the design of these facilities is maximizing the efficiency of available space. Here we report on the effects of aquarium chamber volume on the reproduction of zebrafish, with the objective of identifying the minimal volume required for normal egg production. Six adults (two males and four females) were tested in chamber volumes of 500, 400, 300, 200, and 100 mL. Results were compared with those from a control volume of 3.5 L. Eggs were removed from the test chambers after spawning and incubated in petri dishes at 28°C. Total egg production, percent of eggs hatching, and larval length at 96 h postfertilization were used to evaluate breeding success. Compared with the control...
{"title":"Chamber Volume Requirements for Reproduction of the Zebrafish Danio rerio","authors":"E. Goolish, R. Evans, K. Okutake, Rachael Max","doi":"10.1577/1548-8640(1998)060<0127:CVRFRO>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0127:CVRFRO>2.0.CO;2","url":null,"abstract":"Abstract The zebrafish or zebra danio Danio (=Brachydanio) rerio has recently become a major vertebrate model for the study of developmental biology, neurobiology, and molecular genetics. As a result, most research universities have now invested considerable resources in the construction of large zebrafish facilities. A key element in the design of these facilities is maximizing the efficiency of available space. Here we report on the effects of aquarium chamber volume on the reproduction of zebrafish, with the objective of identifying the minimal volume required for normal egg production. Six adults (two males and four females) were tested in chamber volumes of 500, 400, 300, 200, and 100 mL. Results were compared with those from a control volume of 3.5 L. Eggs were removed from the test chambers after spawning and incubated in petri dishes at 28°C. Total egg production, percent of eggs hatching, and larval length at 96 h postfertilization were used to evaluate breeding success. Compared with the control...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"1 1","pages":"127-132"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79887847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0149:DONOAF>2.0.CO;2
D. D. Theisen, D. Stansell, L. Woods
Abstract The use of ozone to improve water quality in recirculation aquaculture systems is widespread. In these same systems, the use of brine shrimp Artemia spp. as the first food item for larval fish is also very common. The potential of brine shrimp to inoculate culture water with pathogenic bacteria is well understood, and the increasing availability of ozone makes it reasonable to consider ozone as a potential disinfectant of brine shrimp. In this study, brine shrimp nauplii were exposed to ozone (0.75 g/h) for various periods (10, 15, 20, 25, and 30 min). Survival of nauplii was greater than 90% in all but the 30-min exposure (84.4%), and bacterial reduction was nearly 100% for all exposures. Ozone, because of its demonstrated potential to disinfect brine shrimp without causing significant mortality, is a viable alternative to chemical disinfection of nauplii before feeding to larval fish.
{"title":"Disinfection of Nauplii of Artemia franciscana by Ozonation","authors":"D. D. Theisen, D. Stansell, L. Woods","doi":"10.1577/1548-8640(1998)060<0149:DONOAF>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0149:DONOAF>2.0.CO;2","url":null,"abstract":"Abstract The use of ozone to improve water quality in recirculation aquaculture systems is widespread. In these same systems, the use of brine shrimp Artemia spp. as the first food item for larval fish is also very common. The potential of brine shrimp to inoculate culture water with pathogenic bacteria is well understood, and the increasing availability of ozone makes it reasonable to consider ozone as a potential disinfectant of brine shrimp. In this study, brine shrimp nauplii were exposed to ozone (0.75 g/h) for various periods (10, 15, 20, 25, and 30 min). Survival of nauplii was greater than 90% in all but the 30-min exposure (84.4%), and bacterial reduction was nearly 100% for all exposures. Ozone, because of its demonstrated potential to disinfect brine shrimp without causing significant mortality, is a viable alternative to chemical disinfection of nauplii before feeding to larval fish.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"38 1","pages":"149-151"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84357232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0133:OSSOYP>2.0.CO;2
S. Kolkovski, K. Dąbrowski
Abstract The thermal and light conditions for 2-year-old broodstock of yellow perch Perca flavescens were shifted by 6 months in an effort to change spawning from spring to autumn and fall. The natural photothermal conditions consisted of a chill period of 10°C and 10 h of light during January–March followed by increases in temperature to 20–22°C and photoperiod to 15 h light during July–September. The natural spawning of yellow perch occurs in April–May at 12–14°C and 12 h light. In the present study, the broodstock were maintained at a higher temperature and a longer period of light during September–February (18°C and 13 h light). The photothermal conditions were decreased gradually until June. The chill period (10°C and 11 h light) was 60 d in duration (June–July) and was followed by gradually increased water temperature and longer daylight (12°C and 19 h). Following this period, 50% of the females were fully ovulated, and they were stripped or spawned naturally. The males spermiated during the entire ...
{"title":"Off-Season Spawning of Yellow Perch","authors":"S. Kolkovski, K. Dąbrowski","doi":"10.1577/1548-8640(1998)060<0133:OSSOYP>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0133:OSSOYP>2.0.CO;2","url":null,"abstract":"Abstract The thermal and light conditions for 2-year-old broodstock of yellow perch Perca flavescens were shifted by 6 months in an effort to change spawning from spring to autumn and fall. The natural photothermal conditions consisted of a chill period of 10°C and 10 h of light during January–March followed by increases in temperature to 20–22°C and photoperiod to 15 h light during July–September. The natural spawning of yellow perch occurs in April–May at 12–14°C and 12 h light. In the present study, the broodstock were maintained at a higher temperature and a longer period of light during September–February (18°C and 13 h light). The photothermal conditions were decreased gradually until June. The chill period (10°C and 11 h light) was 60 d in duration (June–July) and was followed by gradually increased water temperature and longer daylight (12°C and 19 h). Following this period, 50% of the females were fully ovulated, and they were stripped or spawned naturally. The males spermiated during the entire ...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"6 1","pages":"133-136"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76007467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0081:EORHAT>2.0.CO;2
C. Sharpe, D. A. Thompson, H. Blankenship, C. Schreck
Abstract Juvenile chinook salmon Oncorhynchus tshawytscha were subjected to handling and tagging protocols typical of normal hatchery operations and monitored for their physiological response to stress. Treatments included coded-wire-tagging, counting, ventral fin clipping, adipose fin clipping, and a procedure simulating a pond split. Treatment fish were also subjected to a standardized stress challenge (1 h confinement) to evaluate their ability to deal with disturbances subsequent to a handling or tagging procedure. Circulating levels of cortisol and glucose were used as indicators of stress. Each of the treatments elicited very similar responses among treatment groups. Cortisol increased from resting levels of about 20 ng/mL to about 90 ng/mL by 1 h poststress and returned to near resting levels by 8 h poststress. Glucose levels increased from 50 mg/dL to about 80 mg/dL by 1 h poststress and remained elevated for much of the experiment. The cortisol and glucose responses to the confinement stress did ...
{"title":"Effects of Routine Handling and Tagging Procedures on Physiological Stress Responses in Juvenile Chinook Salmon","authors":"C. Sharpe, D. A. Thompson, H. Blankenship, C. Schreck","doi":"10.1577/1548-8640(1998)060<0081:EORHAT>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0081:EORHAT>2.0.CO;2","url":null,"abstract":"Abstract Juvenile chinook salmon Oncorhynchus tshawytscha were subjected to handling and tagging protocols typical of normal hatchery operations and monitored for their physiological response to stress. Treatments included coded-wire-tagging, counting, ventral fin clipping, adipose fin clipping, and a procedure simulating a pond split. Treatment fish were also subjected to a standardized stress challenge (1 h confinement) to evaluate their ability to deal with disturbances subsequent to a handling or tagging procedure. Circulating levels of cortisol and glucose were used as indicators of stress. Each of the treatments elicited very similar responses among treatment groups. Cortisol increased from resting levels of about 20 ng/mL to about 90 ng/mL by 1 h poststress and returned to near resting levels by 8 h poststress. Glucose levels increased from 50 mg/dL to about 80 mg/dL by 1 h poststress and remained elevated for much of the experiment. The cortisol and glucose responses to the confinement stress did ...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"48 1","pages":"81-87"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77166136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0114:FEIICW>2.0.CO;2
R. Clayton, T. Stevenson, R. Summerfelt
Abstract Fin erosion was observed in two cohorts of walleyes Stizostedion vitreum and one cohort of half-sibling hybrid walleyes (walleye × sauger S. canadense) raised from hatch in fiberglass tanks. All size and relative fin length (fin length/standard length) differences between stocks within the same year were statistically significant. Morphometric measurements of the 1993 cohorts of walleyes and half-sibling hybrids were made in 1994 at 414 d posthatch (dph). Caudal fin erosion was more severe in walleyes than in hybrids, but hybrid walleyes had more severe pectoral and pelvic fin erosion. Also, compared with mean relative fin lengths from reference sources, pelvic fins of walleyes were about 45% of the reference values versus 28% for hybrids; relative pectoral fin length for walleyes was 78% but only 28% for hybrids; relative caudal fin length was 94.9% for walleyes and 130% for hybrids. In the 1995 cohorts, we compared measurements of 206-dph walleyes from Spirit Lake, Iowa (SLW), with 227-dph dome...
摘要对玻璃钢槽中孵化的两组黄颡鱼(walleye)和一组半同胞杂交黄颡鱼(walleye × sauger S. canada)进行了鳍侵蚀试验。同一年内各种群的体型和相对鳍长(鳍长/标准长度)差异均具有统计学意义。于1994年对1993年的长眼鱼和同父异母杂交品种进行了形态测量。尾鳍糜烂程度以杂交种高，胸鳍和腹鳍糜烂程度以杂交种高。此外，与参考来源的平均相对鳍长相比，斑眼鱼的腹鳍约为参考值的45%，而杂交鱼为28%;斑眼鱼胸鳍相对长度为78%，杂交种仅为28%;尾鳍的相对长度为94.9%，杂交种为130%。在1995年的队列中，我们比较了爱荷华州精神湖(SLW) 206-dph的瓦利眼与227-dph的圆眼的测量结果。
{"title":"Fin Erosion in Intensively Cultured Walleyes and Hybrid Walleyes","authors":"R. Clayton, T. Stevenson, R. Summerfelt","doi":"10.1577/1548-8640(1998)060<0114:FEIICW>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0114:FEIICW>2.0.CO;2","url":null,"abstract":"Abstract Fin erosion was observed in two cohorts of walleyes Stizostedion vitreum and one cohort of half-sibling hybrid walleyes (walleye × sauger S. canadense) raised from hatch in fiberglass tanks. All size and relative fin length (fin length/standard length) differences between stocks within the same year were statistically significant. Morphometric measurements of the 1993 cohorts of walleyes and half-sibling hybrids were made in 1994 at 414 d posthatch (dph). Caudal fin erosion was more severe in walleyes than in hybrids, but hybrid walleyes had more severe pectoral and pelvic fin erosion. Also, compared with mean relative fin lengths from reference sources, pelvic fins of walleyes were about 45% of the reference values versus 28% for hybrids; relative pectoral fin length for walleyes was 78% but only 28% for hybrids; relative caudal fin length was 94.9% for walleyes and 130% for hybrids. In the 1995 cohorts, we compared measurements of 206-dph walleyes from Spirit Lake, Iowa (SLW), with 227-dph dome...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"46 1","pages":"114-118"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81129415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0101:EOFOGS>2.0.CO;2
L. R. Martínez-Córdova, N. Pasten-Miranda, R. Barraza-Guardado
Abstract A 19-week study was conducted in Bahia Kino, Sonora, Mexico, to evaluate the effects of fertilization on growth, survival, food conversion ratio, and production of Pacific white shrimp Penaeus vannamei in earthen ponds. The contribution of phytoplankton, zooplankton, and benthos to shrimp food supply was assessed by examining changes in the abundance of these organisms in the ponds, as well as in shrimp gut content. Shrimp growth and biomass were higher in fertilized ponds, despite a lower feeding rate. These results suggest that fertilization enhances natural food and contributes to shrimp nutrition. Biota were more abundant in fertilized ponds. Guts of shrimp from fertilized ponds had significantly higher abundances of zooplankton and benthic organisms than guts of shrimp from unfertilized ponds.
{"title":"Effect of Fertilization on Growth, Survival, Food Conversion Ratio, and Production of Pacific White Shrimp Penaeus vannamei in Earthen Ponds in Sonora, Mexico","authors":"L. R. Martínez-Córdova, N. Pasten-Miranda, R. Barraza-Guardado","doi":"10.1577/1548-8640(1998)060<0101:EOFOGS>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0101:EOFOGS>2.0.CO;2","url":null,"abstract":"Abstract A 19-week study was conducted in Bahia Kino, Sonora, Mexico, to evaluate the effects of fertilization on growth, survival, food conversion ratio, and production of Pacific white shrimp Penaeus vannamei in earthen ponds. The contribution of phytoplankton, zooplankton, and benthos to shrimp food supply was assessed by examining changes in the abundance of these organisms in the ponds, as well as in shrimp gut content. Shrimp growth and biomass were higher in fertilized ponds, despite a lower feeding rate. These results suggest that fertilization enhances natural food and contributes to shrimp nutrition. Biota were more abundant in fertilized ponds. Guts of shrimp from fertilized ponds had significantly higher abundances of zooplankton and benthic organisms than guts of shrimp from unfertilized ponds.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"109 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75002481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0146:COSFAA>2.0.CO;2
I. Babiak, J. Glogowski, R. Kujawa, D. Kucharczyk, A. Mamcarz
Abstract This is the first report on sperm cryopreservation for the cyprinid species asp Aspius aspius. Fish were captured from wild stock and kept under controlled conditions. Ovulation and spermiation were induced with hormonal treatment. Six simple carbohydrate-based extenders supplemented with 10% DMSO were tested for sperm cryopreservation. The most efficient extenders were 0.3 M sucrose, 0.6 M sucrose, and 0.3 M glucose. They resulted in survival to the eyed stage of 62 ± 4%, 59 ± 5%, and 49 ± 4%, respectively, expressed as a percentage of control survival, which was 58 ± 6%. The presence of chicken egg yolk in extenders significantly decreased cryopreservation success.
{"title":"Cryopreservation of Sperm from Asp Aspius aspius","authors":"I. Babiak, J. Glogowski, R. Kujawa, D. Kucharczyk, A. Mamcarz","doi":"10.1577/1548-8640(1998)060<0146:COSFAA>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0146:COSFAA>2.0.CO;2","url":null,"abstract":"Abstract This is the first report on sperm cryopreservation for the cyprinid species asp Aspius aspius. Fish were captured from wild stock and kept under controlled conditions. Ovulation and spermiation were induced with hormonal treatment. Six simple carbohydrate-based extenders supplemented with 10% DMSO were tested for sperm cryopreservation. The most efficient extenders were 0.3 M sucrose, 0.6 M sucrose, and 0.3 M glucose. They resulted in survival to the eyed stage of 62 ± 4%, 59 ± 5%, and 49 ± 4%, respectively, expressed as a percentage of control survival, which was 58 ± 6%. The presence of chicken egg yolk in extenders significantly decreased cryopreservation success.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"9 1","pages":"146-148"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73486152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0109:RROHPS>2.0.CO;2
J. Tipping
Abstract Sea-run cutthroat trout Oncorhynchus clarki were reared in and released from a pond with a gravel–earth bottom, a standard concrete raceway, and a raceway with baffles at the Cowlitz Trout Hatchery, Washington. Adult returns from two release years showed that significantly more fish from the rearing pond survived (3.2%) than fish reared in standard raceways (2.0%) and that significantly more fish returned from the standard raceway than from the baffled raceway (1.7%).
{"title":"Return Rates of Hatchery-Produced Sea-Run Cutthroat Trout Reared in a Pond versus a Standard or Baffled Raceway","authors":"J. Tipping","doi":"10.1577/1548-8640(1998)060<0109:RROHPS>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0109:RROHPS>2.0.CO;2","url":null,"abstract":"Abstract Sea-run cutthroat trout Oncorhynchus clarki were reared in and released from a pond with a gravel–earth bottom, a standard concrete raceway, and a raceway with baffles at the Cowlitz Trout Hatchery, Washington. Adult returns from two release years showed that significantly more fish from the rearing pond survived (3.2%) than fish reared in standard raceways (2.0%) and that significantly more fish returned from the standard raceway than from the baffled raceway (1.7%).","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"151 1","pages":"109-113"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77755487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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