Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0221:ACOTIV>2.0.CO;2
R. Harrell, W. V. Heukelem, J. Kerby
Abstract Triploidy induction is a technique that allows genetic manipulation of chromosome number to control reproduction and potentially create faster-growing animals; however, most methods for inducing polyploidy are not 100% effective. Using sunshine bass (white bass Morone chrysops ♀ × striped bass M. saxatilis ♂) as a model, we cross-validated the most common verification techniques: DNA staining and fluorescence quantification with a flow cytometer, erythrocyte nuclear volume with a Coulter counter particle size analyzer, silver staining of nucleolar organizer regions (NORs), and cytological karyotyping. Results indicated that the electronic techniques of particle size analysis and flow cytometry were the simplest and quickest methods of validation. The major drawback of both electronic ploidy determination methods is the cost of the equipment required for analysis. Cytological karyotyping was the most accurate method for determining polyploidy because actual chromosome numbers were determined. It w...
{"title":"A Comparison of Triploid Induction Validation Techniques","authors":"R. Harrell, W. V. Heukelem, J. Kerby","doi":"10.1577/1548-8640(1998)060<0221:ACOTIV>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0221:ACOTIV>2.0.CO;2","url":null,"abstract":"Abstract Triploidy induction is a technique that allows genetic manipulation of chromosome number to control reproduction and potentially create faster-growing animals; however, most methods for inducing polyploidy are not 100% effective. Using sunshine bass (white bass Morone chrysops ♀ × striped bass M. saxatilis ♂) as a model, we cross-validated the most common verification techniques: DNA staining and fluorescence quantification with a flow cytometer, erythrocyte nuclear volume with a Coulter counter particle size analyzer, silver staining of nucleolar organizer regions (NORs), and cytological karyotyping. Results indicated that the electronic techniques of particle size analysis and flow cytometry were the simplest and quickest methods of validation. The major drawback of both electronic ploidy determination methods is the cost of the equipment required for analysis. Cytological karyotyping was the most accurate method for determining polyploidy because actual chromosome numbers were determined. It w...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"39 1","pages":"221-226"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86964155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0206:GASOLB>2.0.CO;2
C. Mischke, Joseph E. Morris
Abstract The objective of this study was to develop a procedure for rearing larval bluegills Lepomis macrochirus in tanks. In preliminary experiments, seven commercial diets were fed to larval bluegills from the onset of exogenous feeding to 28 d posthatch. Although all diets were eaten by the larvae, none were observed to pass through the fish's digestive tract, and survival was essentially zero. In the next set of experiments, bluegill larvae survived on commercial diets when they were fed nauplii of brine shrimp Artemia franciscana for an initial 7-d period before being switched to commercial feed. Using this protocol, we compared three feeds (BioKyowa Fry Feed Kyowa B-250, Argent Hatchery Encapsulon Grade II, and Zeigler Larval AP-100) over a 28-d period. There were no significant (α = 0.05) differences in growth (length and weight) among fish fed the three diets at the end of 28 d, but survival was significantly higher for fish fed BioKyowa. In another experiment, we fed BioKyowa to larval bluegills ...
{"title":"Growth and Survival of Larval Bluegills in the Laboratory under Different Feeding Regimes","authors":"C. Mischke, Joseph E. Morris","doi":"10.1577/1548-8640(1998)060<0206:GASOLB>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0206:GASOLB>2.0.CO;2","url":null,"abstract":"Abstract The objective of this study was to develop a procedure for rearing larval bluegills Lepomis macrochirus in tanks. In preliminary experiments, seven commercial diets were fed to larval bluegills from the onset of exogenous feeding to 28 d posthatch. Although all diets were eaten by the larvae, none were observed to pass through the fish's digestive tract, and survival was essentially zero. In the next set of experiments, bluegill larvae survived on commercial diets when they were fed nauplii of brine shrimp Artemia franciscana for an initial 7-d period before being switched to commercial feed. Using this protocol, we compared three feeds (BioKyowa Fry Feed Kyowa B-250, Argent Hatchery Encapsulon Grade II, and Zeigler Larval AP-100) over a 28-d period. There were no significant (α = 0.05) differences in growth (length and weight) among fish fed the three diets at the end of 28 d, but survival was significantly higher for fish fed BioKyowa. In another experiment, we fed BioKyowa to larval bluegills ...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"102 1","pages":"206-213"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88802808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0236:AEPSFH>2.0.CO;2
R. Fulford, D. A. Rutherford
Abstract Design and construction of a portable, inexpensive hatching system for brine shrimp Artemia spp. is described. The system design is based on batch culture methodology, which is easily customized to the feeding requirements of a larval system and optimizes the nutritional value of brine shrimp. This system can be set up quickly and provides stable temperature and aeration under a variety of external conditions. Harvest and separation of nauplii is facilitated by the shape of the culture vessel and placement of a light near the bottom of the vessel. During 14-d feeding trials, mean percent hatch of brine shrimp nauplii was 71% (59–81%) at 24 h and 83% (74–94%) at 28 h. Hatching rate was consistent across a range of initial cyst densities.
{"title":"An Economical, Portable System for Hatching Brine Shrimp Artemia spp","authors":"R. Fulford, D. A. Rutherford","doi":"10.1577/1548-8640(1998)060<0236:AEPSFH>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0236:AEPSFH>2.0.CO;2","url":null,"abstract":"Abstract Design and construction of a portable, inexpensive hatching system for brine shrimp Artemia spp. is described. The system design is based on batch culture methodology, which is easily customized to the feeding requirements of a larval system and optimizes the nutritional value of brine shrimp. This system can be set up quickly and provides stable temperature and aeration under a variety of external conditions. Harvest and separation of nauplii is facilitated by the shape of the culture vessel and placement of a light near the bottom of the vessel. During 14-d feeding trials, mean percent hatch of brine shrimp nauplii was 71% (59–81%) at 24 h and 83% (74–94%) at 28 h. Hatching rate was consistent across a range of initial cyst densities.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"36 1","pages":"236-238"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82722436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0239:UOAFPT>2.0.CO;2
A. Skrzypczak, A. Mamcarz, D. Kucharczyk, R. Kujawa
Abstract A system for transfering live natural zooplankton to net cages containing percid larvae is described. The construction of this system is based on a net cage system routinely used for coregonid larvae. The pump transferred zooplankton in proportion to zooplankton density in a lake and can be adjusted depending on fish size and stocking density. A floating pump transferred up to 55% of available zooplankton to a rearing cage. The percentage of zooplankton groups in the control cage and in the pumping water was similar. Zooplanktonic organisms were not damaged by the pump.
{"title":"Use of a Floating Pump to Collect and Transfer Live Zooplankton as Food for Percid Larvae Reared in Net Cages","authors":"A. Skrzypczak, A. Mamcarz, D. Kucharczyk, R. Kujawa","doi":"10.1577/1548-8640(1998)060<0239:UOAFPT>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0239:UOAFPT>2.0.CO;2","url":null,"abstract":"Abstract A system for transfering live natural zooplankton to net cages containing percid larvae is described. The construction of this system is based on a net cage system routinely used for coregonid larvae. The pump transferred zooplankton in proportion to zooplankton density in a lake and can be adjusted depending on fish size and stocking density. A floating pump transferred up to 55% of available zooplankton to a rearing cage. The percentage of zooplankton groups in the control cage and in the pumping water was similar. Zooplanktonic organisms were not damaged by the pump.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"32 1","pages":"239-241"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75267971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0179:EOTHLS>2.0.CO;2
T. Hoffnagle, A. Fivizzani
Abstract This experiment examined whether photoperiodic changes induce chinook salmon Oncorhynchus tshawytscha to alter various indices of smoltification. The effect of 24 h light, a constant photoperiod of 9 h light: 015 h dark (9L:15D), and a naturally increasing photoperiod on plasma levels of thyroxine and cortisol, hematocrit, condition factor (K), and hepatosomatic index (HSI) was tested during the period of smoltification in chinook salmon. The 24-h-light group grew faster than the other two groups, but was significantly larger than one of the two groups on only three sampling dates. Mean plasma thyroxine and cortisol levels were highest in the natural-photoperiod group and lowest in the 9L:15D group. Mean plasma cortisol levels increased significantly in the increasing and 9L:15D photoperiod groups but were unchanged in the continuous-light group. Mean HSI decreased faster in the natural-photoperiod group than in the other two groups. Mean hematocrit did not change in the natural-photoperiod group...
{"title":"EFFECT OF THREE HATCHERY LIGHTING SCHEMES ON INDICES OF SMOLTIFICATION IN CHINOOK SALMON","authors":"T. Hoffnagle, A. Fivizzani","doi":"10.1577/1548-8640(1998)060<0179:EOTHLS>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0179:EOTHLS>2.0.CO;2","url":null,"abstract":"Abstract This experiment examined whether photoperiodic changes induce chinook salmon Oncorhynchus tshawytscha to alter various indices of smoltification. The effect of 24 h light, a constant photoperiod of 9 h light: 015 h dark (9L:15D), and a naturally increasing photoperiod on plasma levels of thyroxine and cortisol, hematocrit, condition factor (K), and hepatosomatic index (HSI) was tested during the period of smoltification in chinook salmon. The 24-h-light group grew faster than the other two groups, but was significantly larger than one of the two groups on only three sampling dates. Mean plasma thyroxine and cortisol levels were highest in the natural-photoperiod group and lowest in the 9L:15D group. Mean plasma cortisol levels increased significantly in the increasing and 9L:15D photoperiod groups but were unchanged in the continuous-light group. Mean HSI decreased faster in the natural-photoperiod group than in the other two groups. Mean hematocrit did not change in the natural-photoperiod group...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"109 1","pages":"179-191"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74756104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0159:EOIPFH>2.0.CO;2
R. D. Ewing, M. Lewis, J. Sheahan, S. K. Ewing
Abstract Juvenile chinook salmon Oncorhynchus tshawytscha reared at Willamette Hatchery, Oakridge, Oregon, showed nonrandom distributions in both modified Burrows raceways and Michigan raceways. When fish were reared in Burrows raceways at densities of 10–15 kg/m3, largest fish tended to reside near the upstream end of the raceway, but when fish were crowded for sampling, smaller fish were found at the upstream end, and larger fish were found at the downstream end. In Michigan raceways, fish near release size occupied six compartments formed by baffles. Smallest fish were usually found in the upstream and downstream compartments, while largest fish were in the middle compartments. Fish sizes were compared between fish sampled by dip net with and without crowding in all experimental raceways. Only 9 of 49 measurements showed statistical differences in weight between the two methods. We conclude that juvenile chinook salmon distribute in modified Burrows raceways and Michigan raceways in a nonrandom manner,...
{"title":"Evaluation of Inventory Procedures for Hatchery Fish. III. Nonrandom Distributions of Chinook Salmon in Raceways","authors":"R. D. Ewing, M. Lewis, J. Sheahan, S. K. Ewing","doi":"10.1577/1548-8640(1998)060<0159:EOIPFH>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0159:EOIPFH>2.0.CO;2","url":null,"abstract":"Abstract Juvenile chinook salmon Oncorhynchus tshawytscha reared at Willamette Hatchery, Oakridge, Oregon, showed nonrandom distributions in both modified Burrows raceways and Michigan raceways. When fish were reared in Burrows raceways at densities of 10–15 kg/m3, largest fish tended to reside near the upstream end of the raceway, but when fish were crowded for sampling, smaller fish were found at the upstream end, and larger fish were found at the downstream end. In Michigan raceways, fish near release size occupied six compartments formed by baffles. Smallest fish were usually found in the upstream and downstream compartments, while largest fish were in the middle compartments. Fish sizes were compared between fish sampled by dip net with and without crowding in all experimental raceways. Only 9 of 49 measurements showed statistical differences in weight between the two methods. We conclude that juvenile chinook salmon distribute in modified Burrows raceways and Michigan raceways in a nonrandom manner,...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"32 1","pages":"159-166"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84111655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0167:EORDAR>2.0.CO;2
R. D. Ewing, J. Sheahan, M. Lewis, A. Palmisano
Abstract Four brood years of juvenile spring chinook salmon Oncorhynchus tshawytscha were reared in conventional and baffled raceways at various rearing densities and loads at Willamette Hatchery, Oregon. A period of rapid linear growth occurred from August to November, but there was little or no growth from November to March when the fish were released. Both fall and winter growth rates were inversely related to rearing density. Final weight and length were also inversely related to rearing density. No significant relationship between load and any growth variable was observed. Fish reared at lower densities in conventional raceways tended to develop bimodal length distributions in winter and early spring. Fish reared in conventional raceways showed significantly larger growth rates and final lengths and weights than those reared in baffled raceways. Food conversions and average delivery times for feed were significantly greater in baffled than in conventional raceways. No significant relationships were o...
{"title":"Effects of Rearing Density and Raceway Conformation on Growth, Food Conversion, and Survival of Juvenile Spring Chinook Salmon","authors":"R. D. Ewing, J. Sheahan, M. Lewis, A. Palmisano","doi":"10.1577/1548-8640(1998)060<0167:EORDAR>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0167:EORDAR>2.0.CO;2","url":null,"abstract":"Abstract Four brood years of juvenile spring chinook salmon Oncorhynchus tshawytscha were reared in conventional and baffled raceways at various rearing densities and loads at Willamette Hatchery, Oregon. A period of rapid linear growth occurred from August to November, but there was little or no growth from November to March when the fish were released. Both fall and winter growth rates were inversely related to rearing density. Final weight and length were also inversely related to rearing density. No significant relationship between load and any growth variable was observed. Fish reared at lower densities in conventional raceways tended to develop bimodal length distributions in winter and early spring. Fish reared in conventional raceways showed significantly larger growth rates and final lengths and weights than those reared in baffled raceways. Food conversions and average delivery times for feed were significantly greater in baffled than in conventional raceways. No significant relationships were o...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"134 1","pages":"167-178"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77394394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-06-01DOI: 10.1577/1548-8640(1998)060<0200:SAGOTS>2.0.CO;2
F. Thrower, R. Martin, R. Heintz
Abstract Two hatchery stocks of juvenile spring chinook salmon Oncorhynchus tshawytscha originating from two southeast Alaska rivers were reared in freshwater to an average size of 8–10 g and placed in marine net-pens with an artificial freshwater lens system (AFLS) at four entry times from mid-September to early November 1987. Overwinter survival to May 1988 averaged 83.6% for Unuk River stock and 76.0% for Chickamin River stock and did not differ significantly in the AFLS among the four entry dates. Survival over 24 h differed significantly when entry was directly into 30‰ seawater. The 24-h plasma sodium levels in the juveniles challenged to either full-strength seawater or marine net-pens with an AFLS were not useful predictors of overwinter survival. Over winter, both stocks grew significantly better in the AFLS than in freshwater. The AFLS appears to be a cost-effective means of substantially increasing smolt production while minimizing freshwater use.
{"title":"SURVIVAL AND GROWTH OF TWO STOCKS OF PRESMOLT CHINOOK SALMON HELD OVERWINTER IN MARINE NET-PENS WITH AN ARTIFICIAL FRESHWATER LENS","authors":"F. Thrower, R. Martin, R. Heintz","doi":"10.1577/1548-8640(1998)060<0200:SAGOTS>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0200:SAGOTS>2.0.CO;2","url":null,"abstract":"Abstract Two hatchery stocks of juvenile spring chinook salmon Oncorhynchus tshawytscha originating from two southeast Alaska rivers were reared in freshwater to an average size of 8–10 g and placed in marine net-pens with an artificial freshwater lens system (AFLS) at four entry times from mid-September to early November 1987. Overwinter survival to May 1988 averaged 83.6% for Unuk River stock and 76.0% for Chickamin River stock and did not differ significantly in the AFLS among the four entry dates. Survival over 24 h differed significantly when entry was directly into 30‰ seawater. The 24-h plasma sodium levels in the juveniles challenged to either full-strength seawater or marine net-pens with an AFLS were not useful predictors of overwinter survival. Over winter, both stocks grew significantly better in the AFLS than in freshwater. The AFLS appears to be a cost-effective means of substantially increasing smolt production while minimizing freshwater use.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"18 1","pages":"200-205"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89467195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0119:ABACCI>2.0.CO;2
S. Lochmann, P. Perschbacher, G. Merry, N. Fijan
Abstract Observations of marks on channel catfish Ictalurus punctatus held for varying lengths of time in experimental ponds and on fish submitted for disease analysis suggested that aggressive biting among channel catfish may sometimes occur at relatively high rates in culture ponds. Studies in experimental pools indicated that larger fish sustained an average of 4 bites per fish while smaller fish sustained less than 1 bite per fish. Unfed catfish sustained an average of 12 bites per fish while fed fish sustained 7 bites per fish. No differences in the level of aggression were observed between genders. Mortality rates, apparently due to biting, ranged from 0% to 40% during the 2–4 week periods. Biting and subsequent infection of the wounds may contribute to the unexplained losses observed by producers.
{"title":"Aggressive Biting among Channel Catfish in Pool Studies","authors":"S. Lochmann, P. Perschbacher, G. Merry, N. Fijan","doi":"10.1577/1548-8640(1998)060<0119:ABACCI>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0119:ABACCI>2.0.CO;2","url":null,"abstract":"Abstract Observations of marks on channel catfish Ictalurus punctatus held for varying lengths of time in experimental ponds and on fish submitted for disease analysis suggested that aggressive biting among channel catfish may sometimes occur at relatively high rates in culture ponds. Studies in experimental pools indicated that larger fish sustained an average of 4 bites per fish while smaller fish sustained less than 1 bite per fish. Unfed catfish sustained an average of 12 bites per fish while fed fish sustained 7 bites per fish. No differences in the level of aggression were observed between genders. Mortality rates, apparently due to biting, ranged from 0% to 40% during the 2–4 week periods. Biting and subsequent infection of the wounds may contribute to the unexplained losses observed by producers.","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"35 1","pages":"119-126"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84337561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-03-01DOI: 10.1577/1548-8640(1998)060<0095:EODLSO>2.0.CO;2
Neil J. Greenberg, R. Harrell
Abstract Dietary essential fatty acid supplements fed to subadult sunshine bass Morone chrysops ♀ × M. saxatilis ♂ were evaluated for 6 months with four experimental semipurified diets that were formulated with different ratios of eicosapentaenoic and docosahexaenoic acids (EPA and DHA): 0:0 for diet 1 (control), 1:1 for diet 2, 1:2 for diet 3, and 2:1 for diet 4. A commercially available trout grower diet known to support sunshine bass growth was compared as an industry standard. Fish were fed each diet in triplicate, with 25 fish/replicate. Weight was measured every 6 weeks. With the exception of the final weighing, at which fish fed the trout grower diet were larger, there was no significant difference in the weights of the fish from all the diet trials. Fish fed diets supplemented with highly unsaturated fatty acids (HUFA) showed a decline in tissue concentrations of n-3 HUFA over the study period, except in diet 4 (high EPA in diet, elevated EPA body levels) and diets 2, 3, and 4 (high DHA in diet, e...
{"title":"Effects of Dietary Lipid Supplements on Subadult Sunshine Bass","authors":"Neil J. Greenberg, R. Harrell","doi":"10.1577/1548-8640(1998)060<0095:EODLSO>2.0.CO;2","DOIUrl":"https://doi.org/10.1577/1548-8640(1998)060<0095:EODLSO>2.0.CO;2","url":null,"abstract":"Abstract Dietary essential fatty acid supplements fed to subadult sunshine bass Morone chrysops ♀ × M. saxatilis ♂ were evaluated for 6 months with four experimental semipurified diets that were formulated with different ratios of eicosapentaenoic and docosahexaenoic acids (EPA and DHA): 0:0 for diet 1 (control), 1:1 for diet 2, 1:2 for diet 3, and 2:1 for diet 4. A commercially available trout grower diet known to support sunshine bass growth was compared as an industry standard. Fish were fed each diet in triplicate, with 25 fish/replicate. Weight was measured every 6 weeks. With the exception of the final weighing, at which fish fed the trout grower diet were larger, there was no significant difference in the weights of the fish from all the diet trials. Fish fed diets supplemented with highly unsaturated fatty acids (HUFA) showed a decline in tissue concentrations of n-3 HUFA over the study period, except in diet 4 (high EPA in diet, elevated EPA body levels) and diets 2, 3, and 4 (high DHA in diet, e...","PeriodicalId":22850,"journal":{"name":"The Progressive Fish-culturist","volume":"66 1","pages":"95-100"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90983023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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