Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.396-401
N. A. Samofalova, N. S. Malysheva, N. A. Vagin
Geohelminth infections are a complex and multifaceted problem that attracts the attention of both medical doctors and veterinarians, as well as scientists. They are the most common parasitic diseases. The annual number of recorded cases of geohelminthiasis in humans in the Kursk Region and conditions conducive to their spread have determined the timeliness of studies on contamination of environmental objects with geohelminth eggs. Analysis of the study results showed that the contamination of environmental objects with parasitic agents was 8.4%. Ascaris and Toxocara eggs were recorded in the samples. 7.9% of the examined soil samples, 13.7% of the manure and 3.7% of the runoff samples from the territories were positive for pathogens of geohelminthiasis. Depending on the season of the year, the indicators of environmental objects contaminated with geohelminth eggs were not the same. There was an increase in contamination of the soil and runoff from territories almost twice in the autumn period (prevalence, 12.6%) as compared with the summer period (EO 6.5%). Thus, environmental objects contaminated by parasitic agents indicate the circulation of geohelminthiasis pathogens among domestic animals in the study territories and a risk of infection with larval toxocariasis and ascariasis for the population.
{"title":"ENVIRONMENTAL CONTAMINATION BY PATHOGENS OF GEOHELMINTHIASIS IN THE SOUTH-EAST OF THE KURSK REGION","authors":"N. A. Samofalova, N. S. Malysheva, N. A. Vagin","doi":"10.31016/978-5-6048555-6-0.2023.24.396-401","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.396-401","url":null,"abstract":"Geohelminth infections are a complex and multifaceted problem that attracts the \u0000attention of both medical doctors and veterinarians, as well as scientists. They \u0000are the most common parasitic diseases. The annual number of recorded cases \u0000of geohelminthiasis in humans in the Kursk Region and conditions conducive \u0000to their spread have determined the timeliness of studies on contamination of \u0000environmental objects with geohelminth eggs. Analysis of the study results showed \u0000that the contamination of environmental objects with parasitic agents was 8.4%. \u0000Ascaris and Toxocara eggs were recorded in the samples. 7.9% of the examined soil \u0000samples, 13.7% of the manure and 3.7% of the runoff samples from the territories \u0000were positive for pathogens of geohelminthiasis. Depending on the season of the \u0000year, the indicators of environmental objects contaminated with geohelminth eggs \u0000were not the same. There was an increase in contamination of the soil and runoff from territories almost twice in the autumn period (prevalence, 12.6%) as compared \u0000with the summer period (EO 6.5%). Thus, environmental objects contaminated \u0000by parasitic agents indicate the circulation of geohelminthiasis pathogens among \u0000domestic animals in the study territories and a risk of infection with larval toxocariasis \u0000and ascariasis for the population.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82294292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.493-497
M. Khalikov
The purpose of the research is to study the chemical stability of triclabendazole (TCB) in preparations obtained by its mechanochemical modification with polymeric substances and succinic acid (SA), as well as samples of Triclafascid obtained in 2015–2022 and left for storage at a temperature of 18–25 0C and a relative humidity of 55–60%. To achieve this goal, we selected samples of preparations obtained earlier and in freshly prepared solid dispersions (SD) based on TCB, SA, polyvinylpyrrolidone (PVP) and arabinogalactan (AG). Modification of TCB by polymeric substances was carried out at a weight ratio of components 1:9 in an LE101 ball mill at a rotation speed of 65–70 rpm with sampling for stability (integrity) analysis of TCB by high performance liquid chromatography (HPLC). The integrity analysis of TCB in its SDs obtained in 2015–2022 showed that there was no chemical destruction of TCB in them. The TCB substance is a stable chemical molecule that is not affected by destruction either during machining or during succinic acid treatment. The obtained SDs remain stable for TCB during storage for more than 7 years.
{"title":"STUDYING THE CHEMICAL STABILITY OF TRICLABENDAZOLE-BASED PREPARATIONS","authors":"M. Khalikov","doi":"10.31016/978-5-6048555-6-0.2023.24.493-497","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.493-497","url":null,"abstract":"The purpose of the research is to study the chemical stability of triclabendazole (TCB) \u0000in preparations obtained by its mechanochemical modification with polymeric \u0000substances and succinic acid (SA), as well as samples of Triclafascid obtained in \u00002015–2022 and left for storage at a temperature of 18–25 0C and a relative \u0000humidity of 55–60%. To achieve this goal, we selected samples of preparations \u0000obtained earlier and in freshly prepared solid dispersions (SD) based on TCB, SA, \u0000polyvinylpyrrolidone (PVP) and arabinogalactan (AG). Modification of TCB by \u0000polymeric substances was carried out at a weight ratio of components 1:9 in an LE101 ball mill at a rotation speed of 65–70 rpm with sampling for stability (integrity) \u0000analysis of TCB by high performance liquid chromatography (HPLC). The integrity \u0000analysis of TCB in its SDs obtained in 2015–2022 showed that there was no chemical \u0000destruction of TCB in them. The TCB substance is a stable chemical molecule \u0000that is not affected by destruction either during machining or during succinic acid \u0000treatment. The obtained SDs remain stable for TCB during storage for more than \u00007 years.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82719546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.229-234
N. Kreshchenko, D. E. Mitkovskii
The planarian Schmidtea mediterranea (Turbellaria, Platyhelminthes) has a pair of eyes on the anterior part of the body consisting of pigment cells and photoreceptor neurons capable of regeneration. The research represents a study of the effects of melatonin on photoreceptor differentiation after decapitation of the planarian. During the experiment, the worms were decapitated, and the eye regeneration was observed at days 3 and 4 of the regeneration. Different stages of the photoreceptor regeneration were observed such as both eyes in the regenerating planarians, planarians with one eye, and planarians with no eyes. In most of the animals, the photoreceptors were already visible at day 4 after the decapitation. In the experimental planarians exposed to melatonin at concentrations of 1 and 0.1 µM, the accelerated photoreceptor differentiation was observed. The stimulating effects of melatonin were detected in three animal groups on day 3 and 4 after the decapitation. The study will be continued to evaluate mechanisms of the melatonin action on planarian regeneration.
{"title":"MELATONIN STIMULATES PHOTORECEPTOR DIFFERENTIATION IN REGENERATION OF PLANARIAN SCHMIDTEA MEDITERRANEA","authors":"N. Kreshchenko, D. E. Mitkovskii","doi":"10.31016/978-5-6048555-6-0.2023.24.229-234","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.229-234","url":null,"abstract":"The planarian Schmidtea mediterranea (Turbellaria, Platyhelminthes) has a \u0000pair of eyes on the anterior part of the body consisting of pigment cells and \u0000photoreceptor neurons capable of regeneration. The research represents a study \u0000of the effects of melatonin on photoreceptor differentiation after decapitation of \u0000the planarian. During the experiment, the worms were decapitated, and the eye \u0000regeneration was observed at days 3 and 4 of the regeneration. Different stages of \u0000the photoreceptor regeneration were observed such as both eyes in the regenerating \u0000planarians, planarians with one eye, and planarians with no eyes. In most of the \u0000animals, the photoreceptors were already visible at day 4 after the decapitation. In \u0000the experimental planarians exposed to melatonin at concentrations of 1 and 0.1 \u0000µM, the accelerated photoreceptor differentiation was observed. The stimulating \u0000effects of melatonin were detected in three animal groups on day 3 and 4 after the \u0000decapitation. The study will be continued to evaluate mechanisms of the melatonin \u0000action on planarian regeneration.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88721856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.508-512
I. I. Tsepilova, B. K. Laipanov, I. S. Shcherbakova
The socio-economic role in the development of peasant farming in the current conditions of import substitution will certainly increase, especially in filling local and regional markets with agricultural products. The limiting factor of farms of any form of ownership are parasitic diseases. Studies on the infection of livestock animals were carried out at a peasant farm enterprise of the Dmitrov City District of the Moscow Region. Feces were taken individually from each animal: cattle (70 lactating cows, and 32 young bulls on fattening), goats (15 milk goats, and 12 baby goats kept together), 10 Vietnamese pot–bellied pigs, 2 horses and ponies. Based on the conducted studies, it was found that the cattle, namely the lactating cows and bulls on fattening were infected by pathogens of gastrointestinal strongylatosis by 14.3 and 50%, and the milk and young goats, by 100%, which indicates a high infection rate of these pathogens in ruminants. Also, the nematode Strongyloides papillosus (IP=80%) and protozoa from the genus Eimeria (IP=53%) were identified in the mature goats. In the Vietnamese pot-bellied pigs, horses and ponies, the most common helminth infections were identified, namely: Oesophagostomum (IP=50%) and Ascaris suum (IP=80%) and pathogens from the Strongylata suborder, respectively.
{"title":"INFECTION OF LIVESTOCK ANIMALS WITH PARASITES IN THE CONDITIONS OF PEASANT FARMING IN THE MOSCOW REGION","authors":"I. I. Tsepilova, B. K. Laipanov, I. S. Shcherbakova","doi":"10.31016/978-5-6048555-6-0.2023.24.508-512","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.508-512","url":null,"abstract":"The socio-economic role in the development of peasant farming in the current \u0000conditions of import substitution will certainly increase, especially in filling local \u0000and regional markets with agricultural products. The limiting factor of farms of \u0000any form of ownership are parasitic diseases. Studies on the infection of livestock \u0000animals were carried out at a peasant farm enterprise of the Dmitrov City District \u0000of the Moscow Region. Feces were taken individually from each animal: cattle (70 \u0000lactating cows, and 32 young bulls on fattening), goats (15 milk goats, and 12 baby \u0000goats kept together), 10 Vietnamese pot–bellied pigs, 2 horses and ponies. Based \u0000on the conducted studies, it was found that the cattle, namely the lactating cows \u0000and bulls on fattening were infected by pathogens of gastrointestinal strongylatosis \u0000by 14.3 and 50%, and the milk and young goats, by 100%, which indicates a high \u0000infection rate of these pathogens in ruminants. Also, the nematode Strongyloides \u0000papillosus (IP=80%) and protozoa from the genus Eimeria (IP=53%) were identified \u0000in the mature goats. In the Vietnamese pot-bellied pigs, horses and ponies, the most common helminth infections were identified, namely: Oesophagostomum (IP=50%) \u0000and Ascaris suum (IP=80%) and pathogens from the Strongylata suborder, respectively.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87337323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.112-116
T. Burdukovskaya, Z. Dugarov
Crustaceans of the subclass Branchiura Argulus foliaceus cause argulosis, an invasive disease in fish. Arguluses are temporary ectoparasites. Moving along the surface of the host's body, the crustaceans look for places accessible to blood vessels. After they have sucked blood, they leave the fish. Hemorrhages, wounds and ulcers appear in the affected areas. For free-swimming arguluses, behavioral adaptations are aimed at making the most of meetings with hosts and moving from one host to another. Our study presents data on crustaceans A. foliaceus from the perch from Lake Kenon (Amur River basin). The material was obtained from net catches in the first decade of June 2010. Individuals of the perch of the same size and age groups 2+–4+ were caught at the mouth of the Kadalinka River and in the warm water discharge zone of the Chita CHP-1. According to the results of our research, the relative abundance of argulus in the perch is significantly higher in the area of the mouth of the Kadalinka River than in the warm water discharge zone of the Chita CHP-1. In the springsummer period, the number of crustaceans increases during the spawning of adult fish and the feeding of juveniles in the area of the mouth of the Kadalinka River.
{"title":"ARGULOSIS OF THE PERCH FROM LAKE KENON (ZABAIKALSKY KRAI)","authors":"T. Burdukovskaya, Z. Dugarov","doi":"10.31016/978-5-6048555-6-0.2023.24.112-116","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.112-116","url":null,"abstract":"Crustaceans of the subclass Branchiura Argulus foliaceus cause argulosis, an invasive \u0000disease in fish. Arguluses are temporary ectoparasites. Moving along the surface of \u0000the host's body, the crustaceans look for places accessible to blood vessels. After they \u0000have sucked blood, they leave the fish. Hemorrhages, wounds and ulcers appear in \u0000the affected areas. For free-swimming arguluses, behavioral adaptations are aimed \u0000at making the most of meetings with hosts and moving from one host to another. \u0000Our study presents data on crustaceans A. foliaceus from the perch from Lake Kenon \u0000(Amur River basin). The material was obtained from net catches in the first decade \u0000of June 2010. Individuals of the perch of the same size and age groups 2+–4+ were \u0000caught at the mouth of the Kadalinka River and in the warm water discharge zone of \u0000the Chita CHP-1. According to the results of our research, the relative abundance of \u0000argulus in the perch is significantly higher in the area of the mouth of the Kadalinka \u0000River than in the warm water discharge zone of the Chita CHP-1. In the springsummer period, the number of crustaceans increases during the spawning of adult \u0000fish and the feeding of juveniles in the area of the mouth of the Kadalinka River.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75087802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.475-479
S. Turgunov, A. J. Kaniyazov, E. Shakarboev
One of the common helminth infections of horses in Uzbekistan and other countries is parascaridosis. Infection of horses with the nematode Parascaris equorum (Goeze, 1782) in some regions of our country ranges from 27 to 38%. The purpose of this work is to study the distribution of the nematode Parascaris equorum in the northwestern and eastern regions of Uzbekistan, and the seasonal and age dynamics of infection of horses with this nematode. The studies were carried out in the regions of the Republic of Karakalpakstan and the Ferghana Valley during 2018-2023. One hundred forty-three horses of different age groups in Karakalpakstan and 57 horses in the Fergana Valley were examined by the method of complete and partial helminthological dissections per Skrjabin. Fecal samples from 264 horses were studied using helminthocoprological methods. Parascaris infection rate in the horses examined by the method of complete and partial helminthological dissections averaged 33.6% in the study areas. Helminthocoprological methods were used to study the seasonal and age dynamics of the Parascaris equorum infection in the horses. Seasonal Parascaris equorum infection in the horses was 19.7% in spring, 25.4% in summer, 33.7% in autumn, and 35.2% in winter. Age-related invasiveness of the horses with nematodes was registered at 40.4% in the animals under two years old, 31.4% in the animals from two to five years old, and 24.6% in the animals over five years old.
{"title":"THE DISTRIBUTION OF THE NEMATODE PARASCARIS EQUORUM (GOEZE, 1782) IN THE NORTH-WESTERN AND EASTERN AREAS OF UZBEKISTAN","authors":"S. Turgunov, A. J. Kaniyazov, E. Shakarboev","doi":"10.31016/978-5-6048555-6-0.2023.24.475-479","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.475-479","url":null,"abstract":"One of the common helminth infections of horses in Uzbekistan and other countries \u0000is parascaridosis. Infection of horses with the nematode Parascaris equorum (Goeze, \u00001782) in some regions of our country ranges from 27 to 38%. The purpose of \u0000this work is to study the distribution of the nematode Parascaris equorum in the \u0000northwestern and eastern regions of Uzbekistan, and the seasonal and age dynamics \u0000of infection of horses with this nematode. The studies were carried out in the regions \u0000of the Republic of Karakalpakstan and the Ferghana Valley during 2018-2023. \u0000One hundred forty-three horses of different age groups in Karakalpakstan and 57 \u0000horses in the Fergana Valley were examined by the method of complete and partial \u0000helminthological dissections per Skrjabin. Fecal samples from 264 horses were \u0000studied using helminthocoprological methods. Parascaris infection rate in the horses \u0000examined by the method of complete and partial helminthological dissections \u0000averaged 33.6% in the study areas. Helminthocoprological methods were used to \u0000study the seasonal and age dynamics of the Parascaris equorum infection in the \u0000horses. Seasonal Parascaris equorum infection in the horses was 19.7% in spring, 25.4% in summer, 33.7% in autumn, and 35.2% in winter. Age-related invasiveness \u0000of the horses with nematodes was registered at 40.4% in the animals under two years \u0000old, 31.4% in the animals from two to five years old, and 24.6% in the animals over \u0000five years old.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75094953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.281-284
T. Malyutina
In the last few decades, the attention of researchers has been attracted by endogenous FMRFamide-like neuropeptides found in a number of invertebrates, including species of the Nematoda phylum. A foreign literature review was presented for the functional significance of endogenous FMRFamide-like neuropeptides in locomotor behaviour of root-knot phytonematodes, representatives of the genus Meloidogyne Goldi, 1982, namely, Meloidogyne incognita, M. minor, M. hapla and M. graminicola. In Russia, such studies are not carried out. The main characteristics of phytoparasitic neuropeptides were obtained from the study of genes (flp-genes) that encode these neuropeptides. M. incognita was found to have FMRFamidelike positive immunoreactivity in the central nervous system and 19 flp genes. The Mi-flp-12 and Mi-flp-14 genes encode neuropeptides that stimulate locomotor behaviour, while Mi-flp-32 encodes a neuropeptide that inhibits parasite locomotor behaviour. Nematodes M. incognita and M. hapla were found to have G-proteincoupled receptors (GPCRs) encoded by the flp-32 gene, and their similarity to receptor 1 (C26F1) of the free-living nematode Caenorhabditis elegans was detected. Similar data were presented in the literature for M. graminicola. The peptidergic signaling nervous system of root-knot phytonematodes is similar to the system of nematodes in vertebrates and free-living nematodes, which indicates the conservatism of the system in species of the entire Nematoda phylum.
在过去的几十年里，研究人员的注意力被在许多无脊椎动物(包括线虫门的物种)中发现的内源性fmrfamily样神经肽所吸引。国外文献综述了内源性fmrfamily样神经肽在根结植物(Meloidogyne Goldi, 1982，即Meloidogyne incognita, M. minor, M. hapla和M. graminicola)运动行为中的功能意义。在俄罗斯，没有进行这样的研究。植物寄生神经肽的主要特征是通过对编码这些神经肽的基因(flp基因)的研究获得的。在中枢神经系统和19个flp基因中发现M. incognita具有fmrfamily样阳性免疫反应性。Mi-flp-12和Mi-flp-14基因编码刺激运动行为的神经肽，而Mi-flp-32基因编码抑制寄生虫运动行为的神经肽。发现隐密线虫(M. incognita)和hapla线虫(M. hapla)具有由flp-32基因编码的g蛋白偶联受体(gpcr)，并检测了它们与秀丽隐杆线虫(Caenorhabditis elegans)的受体1 (C26F1)的相似性。文献中也有类似的数据。根结线虫的肽能信号神经系统与脊椎动物和自由生活线虫的系统相似，表明该系统在整个线虫门物种中的保守性。
{"title":"NEUROPEPTIDES INVOLVING IN THE REGULATION OF LOCOMOTOR BEHAVIOR OF ROOT-KNOT PLANT-PARASITIC NEMATODES (REVIEW)","authors":"T. Malyutina","doi":"10.31016/978-5-6048555-6-0.2023.24.281-284","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.281-284","url":null,"abstract":"In the last few decades, the attention of researchers has been attracted by endogenous \u0000FMRFamide-like neuropeptides found in a number of invertebrates, including \u0000species of the Nematoda phylum. A foreign literature review was presented for \u0000the functional significance of endogenous FMRFamide-like neuropeptides in \u0000locomotor behaviour of root-knot phytonematodes, representatives of the genus \u0000Meloidogyne Goldi, 1982, namely, Meloidogyne incognita, M. minor, M. hapla and \u0000M. graminicola. In Russia, such studies are not carried out. The main characteristics \u0000of phytoparasitic neuropeptides were obtained from the study of genes (flp-genes) \u0000that encode these neuropeptides. M. incognita was found to have FMRFamidelike positive immunoreactivity in the central nervous system and 19 flp genes. The \u0000Mi-flp-12 and Mi-flp-14 genes encode neuropeptides that stimulate locomotor \u0000behaviour, while Mi-flp-32 encodes a neuropeptide that inhibits parasite locomotor \u0000behaviour. Nematodes M. incognita and M. hapla were found to have G-proteincoupled receptors (GPCRs) encoded by the flp-32 gene, and their similarity \u0000to receptor 1 (C26F1) of the free-living nematode Caenorhabditis elegans was \u0000detected. Similar data were presented in the literature for M. graminicola. The \u0000peptidergic signaling nervous system of root-knot phytonematodes is similar to the \u0000system of nematodes in vertebrates and free-living nematodes, which indicates the \u0000conservatism of the system in species of the entire Nematoda phylum.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75619610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.78-84
I. Berezinskaya, S. A. Nagorniy, А. V. Aleshukina, I. Martyusheva
Currently, the diagnosis of helminthic diseases is based on microscopic observation of various stages of parasites, but microscopy is subjective and directly related to the competence of the researcher. Against this backdrop, researchers have described laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) as a potential innovative proteomic analysis tool for helminth identification and differentiation. The purpose of the research is to search for alternative consumables (lysis buffers) in connection with import substitution. For the study, the head ends of Ascaris lumbricoides, male and female (5 specimens each) were taken. The comparative protein profile of the ascarids by mass/charge ratio in the Flex analysis program revealed similar graphs of the head parts of the askaris (male and female) processed with lysis buffer from the Sepsityper Kit 50 and with buffer from the PCR kit. The results of the study show the possibility of species differentiation of nematodes using the MALDI-TOF MS method. Coincidences of informational data were found, which makes it possible to subsequently replace the lysis buffers. New approaches have been studied for more thorough processing of spectra and their visualization using the MALDI Biotyper software. The probability of replacing the lysis buffer from the Sepsityper Kit 50 with the lysis buffer used in PCR was shown.
{"title":"MODIFICATIONS OF THE ASCARIS IDENTIFICATION METHOD BASED ON MASS SPECTROMETRY","authors":"I. Berezinskaya, S. A. Nagorniy, А. V. Aleshukina, I. Martyusheva","doi":"10.31016/978-5-6048555-6-0.2023.24.78-84","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.78-84","url":null,"abstract":"Currently, the diagnosis of helminthic diseases is based on microscopic observation \u0000of various stages of parasites, but microscopy is subjective and directly related to the \u0000competence of the researcher. Against this backdrop, researchers have described \u0000laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) \u0000as a potential innovative proteomic analysis tool for helminth identification and \u0000differentiation. The purpose of the research is to search for alternative consumables \u0000(lysis buffers) in connection with import substitution. For the study, the head \u0000ends of Ascaris lumbricoides, male and female (5 specimens each) were taken. The \u0000comparative protein profile of the ascarids by mass/charge ratio in the Flex analysis \u0000program revealed similar graphs of the head parts of the askaris (male and female) \u0000processed with lysis buffer from the Sepsityper Kit 50 and with buffer from the PCR kit. The results of the study show the possibility of species differentiation of \u0000nematodes using the MALDI-TOF MS method. Coincidences of informational \u0000data were found, which makes it possible to subsequently replace the lysis buffers. \u0000New approaches have been studied for more thorough processing of spectra and their \u0000visualization using the MALDI Biotyper software. The probability of replacing the \u0000lysis buffer from the Sepsityper Kit 50 with the lysis buffer used in PCR was shown.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73592058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.370-375
A. Plieva, R. I. Balayeva
Crenosomiasis is an invasive disease of carnivores caused by parasitizing in the bronchi and trachea of nematodes Crenosoma vulpis, C. taiga, C. petrowi from the genus Crenosoma Molin, 1861, family Crenosomatidae Schulz, 1951, and superfamily Metastrongyloidea Lane, 1917. We have studied vertebrates of wild fauna and synanthropic animals for parasitizing of helminths of the genus Crenosoma Molin, 1861 in the adult and larval stages. Wild vertebrates (jackals, foxes, wolves, wild boars) were delivered to us by hunters usually in autumn and winter. The studies were carried out using the method of complete and partial helminthological dissections per K. I. Skryabin. The obtained helminths were fixed as follows: trematodes in 70% alcohol, nematodes in Barbagallo fluid, and labeled. The total infection of animals with Crenosoma was 83.3%. Severe infection was detected in foxes (83.3%) and hedgehogs (100%). Among wild vertebrates, one specimen of Sus scrofa was examined by partial helminthological dissection, in whose lungs we identified nematode Crenosoma sp. for the first time in the Republic of Ingushetia. When analyzing the literature sources on the infection of wild boars with this type of helminth in Russia, no data were found. Crenosoma sp. has been recorded in the wild boar in Russia for the first time.
{"title":"DETECTION OF REPRESENTATIVES OF THE GENUS CRENOSOMA MOLIN, 1861 IN WILD AND SYNANTHROPIC VERTEBRATES","authors":"A. Plieva, R. I. Balayeva","doi":"10.31016/978-5-6048555-6-0.2023.24.370-375","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.370-375","url":null,"abstract":"Crenosomiasis is an invasive disease of carnivores caused by parasitizing in the \u0000bronchi and trachea of nematodes Crenosoma vulpis, C. taiga, C. petrowi from \u0000the genus Crenosoma Molin, 1861, family Crenosomatidae Schulz, 1951, and \u0000superfamily Metastrongyloidea Lane, 1917. We have studied vertebrates of wild \u0000fauna and synanthropic animals for parasitizing of helminths of the genus Crenosoma \u0000Molin, 1861 in the adult and larval stages. Wild vertebrates (jackals, foxes, wolves, \u0000wild boars) were delivered to us by hunters usually in autumn and winter. The \u0000studies were carried out using the method of complete and partial helminthological \u0000dissections per K. I. Skryabin. The obtained helminths were fixed as follows: \u0000trematodes in 70% alcohol, nematodes in Barbagallo fluid, and labeled. The total \u0000infection of animals with Crenosoma was 83.3%. Severe infection was detected in \u0000foxes (83.3%) and hedgehogs (100%). Among wild vertebrates, one specimen of \u0000Sus scrofa was examined by partial helminthological dissection, in whose lungs we \u0000identified nematode Crenosoma sp. for the first time in the Republic of Ingushetia. \u0000When analyzing the literature sources on the infection of wild boars with this type of \u0000helminth in Russia, no data were found. Crenosoma sp. has been recorded in the wild \u0000boar in Russia for the first time.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82161949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.23-34
A. Uspensky, M. Arisov, O. Panova
Konstantin Ivanovich Skryabin is an outstanding Helminthologist, Academician of three Academies of Sciences (V. I. Lenin All-Union Academy of Agricultural Sciences, USSR Academy of Sciences, USSR Academy of Medical Sciences), Hero of Socialist Labor, Lenin and Stalin (twice) prize winner, and Recipient of six Orders of Lenin. In 2023, the 145th Anniversary of his birth will be celebrated on December 7, 1878. Konstantin Ivanovich received the degree of Doctor of Veterinary Medicine in 1905 at the Yuryev Veterinary Institute (now the University of Tartu, Estonia). After completing his studies until 1911, he worked as a local city veterinarian for the first 2 years in Chimkent, and then in Aulie-Ata (later Dzhambul, now Taraz) of Turkestan where he collected an extensive helminthological collection. From 1912 to 1914 he worked on probation in the field of helminthology abroad, namely, in Germany, Switzerland, and France. Upon returning to Russia, he remained to work at the Veterinary Laboratory of the Ministry of Internal Affairs in St. Petersburg. From 1917 to 1920, K. I. Skryabin became the first Professor of the first Department of Parasitology in Russia at the Don Veterinary Institute in Novocherkassk. He organized not only pedagogical, but also scientific work; the idea of organizing helminthological expeditions was born here. In 1920, upon his return to Moscow, he became the Head of the Helminthological Department of the State Institute of Experimental Veterinary Medicine (SIEVM). All subsequent vigorous scientific activity of Konstantin Ivanovich was aimed at strengthening the positions of helminthology as a science in Russia, and expanding the network of educational and scientific organizations. K. I. Skryabin and his students created a huge number of works: articles and monographs. His publications list includes about 700 titles including a number of fundamental multivolume papers. Konstantin Ivanovich Skryabin died on October 17, 1972. He was buried at the Novo-Dyevitchiye cemetery in Moscow.
康斯坦丁·伊万诺维奇·斯克里亚宾是一位杰出的血吸虫学家，三个科学院院士(列宁全联盟农业科学院、苏联科学院、苏联医学科学院)，社会主义劳动英雄，列宁和斯大林奖(两次)获得者，六次列宁勋章获得者。2023年，他的145周年诞辰将于1878年12月7日举行。康斯坦丁·伊万诺维奇于1905年在尤里耶夫兽医学院(现为爱沙尼亚塔尔图大学)获得兽医博士学位。完成学业后，直到1911年，他在奇姆肯特担任当地城市兽医两年，然后在突厥斯坦的Aulie-Ata(后来的Dzhambul，现在的Taraz)收集了大量的蠕虫标本。从1912年到1914年，他在国外，即德国、瑞士和法国，从事蠕虫学领域的见习工作。回到俄罗斯后，他继续在圣彼得堡内务部兽医实验室工作。从1917年到1920年，K. I. Skryabin成为俄罗斯新切尔卡斯克顿河兽医研究所第一个寄生虫学部门的第一位教授。他不仅组织教学工作，而且组织科学工作;组织蠕虫学考察的想法就是在这里诞生的。1920年，回到莫斯科后，他成为国家实验兽医研究所(SIEVM)寄生虫学系的主任。康斯坦丁·伊万诺维奇随后的所有积极的科学活动都是为了加强蠕虫学在俄罗斯作为一门科学的地位，扩大教育和科学组织的网络。K. I. Skryabin和他的学生们创作了大量的作品:文章和专著。他的出版物列表包括大约700个标题，其中包括一些基本的多卷论文。康斯坦丁·伊万诺维奇·斯克里亚宾死于1972年10月17日。他被安葬在莫斯科的novo - dye - chiye公墓。
{"title":"145th ANNIVERSARY OF THE BIRTH OF KONSTANTIN IVANOVICH SKRYABIN (1878-1972)","authors":"A. Uspensky, M. Arisov, O. Panova","doi":"10.31016/978-5-6048555-6-0.2023.24.23-34","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.23-34","url":null,"abstract":"Konstantin Ivanovich Skryabin is an outstanding Helminthologist, Academician \u0000of three Academies of Sciences (V. I. Lenin All-Union Academy of Agricultural \u0000Sciences, USSR Academy of Sciences, USSR Academy of Medical Sciences), Hero \u0000of Socialist Labor, Lenin and Stalin (twice) prize winner, and Recipient of six Orders \u0000of Lenin. In 2023, the 145th Anniversary of his birth will be celebrated on December \u00007, 1878. Konstantin Ivanovich received the degree of Doctor of Veterinary Medicine \u0000in 1905 at the Yuryev Veterinary Institute (now the University of Tartu, Estonia). \u0000After completing his studies until 1911, he worked as a local city veterinarian for the \u0000first 2 years in Chimkent, and then in Aulie-Ata (later Dzhambul, now Taraz) of \u0000Turkestan where he collected an extensive helminthological collection. From 1912 to 1914 he worked on probation in the field of helminthology abroad, namely, in \u0000Germany, Switzerland, and France. Upon returning to Russia, he remained to work \u0000at the Veterinary Laboratory of the Ministry of Internal Affairs in St. Petersburg. \u0000From 1917 to 1920, K. I. Skryabin became the first Professor of the first Department \u0000of Parasitology in Russia at the Don Veterinary Institute in Novocherkassk. He \u0000organized not only pedagogical, but also scientific work; the idea of organizing \u0000helminthological expeditions was born here. In 1920, upon his return to Moscow, \u0000he became the Head of the Helminthological Department of the State Institute of \u0000Experimental Veterinary Medicine (SIEVM). All subsequent vigorous scientific \u0000activity of Konstantin Ivanovich was aimed at strengthening the positions of \u0000helminthology as a science in Russia, and expanding the network of educational \u0000and scientific organizations. K. I. Skryabin and his students created a huge number \u0000of works: articles and monographs. His publications list includes about 700 titles \u0000including a number of fundamental multivolume papers. Konstantin Ivanovich \u0000Skryabin died on October 17, 1972. He was buried at the Novo-Dyevitchiye \u0000cemetery in Moscow.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80940817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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