Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.146-150
V. N. Gerasimov, E. M. Aslanyan, A. Tyryshkina, N. A. Leontyeva
In the prevention of parasitic diseases, an important place is occupied by the search for new highly effective chemicals for the disinfection of environmental objects. When selecting disinfectants, helminth eggs are often used as test objects. The availability of high-quality and standard test material contributes greatly to successful research. However, publications devoted to the methodological side of this problem are clearly not enough. Ascaridata eggs (Ascaris suum, Toxocara canis, etc.) are the most resistant to chemicals, so testing on them allows us to identify the most effective means for the purposes of disinfection. Special attention should be paid to such an object as Enterobius vermicularis, the causative agent of enterobiasis, the most common of helminth infections in humans. This paper describes methods for obtaining standard egg cultures of such test objects as A. suum and E. vermicularis. A more standard A. suum egg culture is facilitated by material collected only from females with high egg viability, as well as the destruction of egg conglomerates in a suspension using sodium hypochlorite. Standardization of the E. vermicularis egg culture is only achieved by taking samples containing healthy viable eggs after the preview.
{"title":"OBTAINING STANDARD HELMINTH EGG CULTURES FOR STUDYING THE OVICIDAL EFFICACY OF CHEMICALS","authors":"V. N. Gerasimov, E. M. Aslanyan, A. Tyryshkina, N. A. Leontyeva","doi":"10.31016/978-5-6048555-6-0.2023.24.146-150","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.146-150","url":null,"abstract":"In the prevention of parasitic diseases, an important place is occupied by the search \u0000for new highly effective chemicals for the disinfection of environmental objects. When \u0000selecting disinfectants, helminth eggs are often used as test objects. The availability \u0000of high-quality and standard test material contributes greatly to successful research. \u0000However, publications devoted to the methodological side of this problem are clearly \u0000not enough. Ascaridata eggs (Ascaris suum, Toxocara canis, etc.) are the most resistant \u0000to chemicals, so testing on them allows us to identify the most effective means for \u0000the purposes of disinfection. Special attention should be paid to such an object as \u0000Enterobius vermicularis, the causative agent of enterobiasis, the most common of \u0000helminth infections in humans. This paper describes methods for obtaining standard \u0000egg cultures of such test objects as A. suum and E. vermicularis. A more standard A. \u0000suum egg culture is facilitated by material collected only from females with high egg \u0000viability, as well as the destruction of egg conglomerates in a suspension using sodium \u0000hypochlorite. Standardization of the E. vermicularis egg culture is only achieved by \u0000taking samples containing healthy viable eggs after the preview.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78778726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.225-228
V. Kravchenko, G. Kravchenko, G. Itin
As a result of our own long-term research, a comparative analysis of clinical signs, and laboratory, immunological and instrumental methods for diagnosing dirofilariasis of dogs in the North-Western Caucasus was carried out. As a result of the study, it was found that only a comprehensive diagnosis performed in stages allows us not only to diagnose most accurately both mature nematodes and their microfilariae but also to significantly reduce the economic component. Therefore, when diagnosing dirofilariasis in dogs, it is necessary to take into account only the most specific clinical signs. Laboratory methods should combine both simple express methods and more complex, time-consuming ones. When choosing immunological test systems, it is necessary to take into account not only their economic feasibility, but also the qualitative component, since the main principle of test system operation is the reaction to Dirofilaria antigens contained in the blood of an infected dog. They can be used for both individual and group research. When choosing instrumental diagnostic methods such as CT, MRI, ultrasound, etc., which are aimed at detecting sexually mature nematodes, and due to their high cost and complexity, it is necessary to take into account their expediency based on predicting the disease outcome.
{"title":"DIROFILARIASIS DIAGNOSTICS IN DOGS","authors":"V. Kravchenko, G. Kravchenko, G. Itin","doi":"10.31016/978-5-6048555-6-0.2023.24.225-228","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.225-228","url":null,"abstract":"As a result of our own long-term research, a comparative analysis of clinical \u0000signs, and laboratory, immunological and instrumental methods for diagnosing \u0000dirofilariasis of dogs in the North-Western Caucasus was carried out. As a result \u0000of the study, it was found that only a comprehensive diagnosis performed in stages \u0000allows us not only to diagnose most accurately both mature nematodes and their \u0000microfilariae but also to significantly reduce the economic component. Therefore, \u0000when diagnosing dirofilariasis in dogs, it is necessary to take into account only the \u0000most specific clinical signs. Laboratory methods should combine both simple express \u0000methods and more complex, time-consuming ones. When choosing immunological \u0000test systems, it is necessary to take into account not only their economic feasibility, \u0000but also the qualitative component, since the main principle of test system operation \u0000is the reaction to Dirofilaria antigens contained in the blood of an infected dog. They \u0000can be used for both individual and group research. When choosing instrumental \u0000diagnostic methods such as CT, MRI, ultrasound, etc., which are aimed at detecting \u0000sexually mature nematodes, and due to their high cost and complexity, it is necessary \u0000to take into account their expediency based on predicting the disease outcome.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75856865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.290-294
V. A. Marchenko, E. A. Efremova, M. A. Smertina
One of the problems of herd horse breeding of the Altai Mountains is significant spread of helminth infections. The purpose of this research is to characterize the epizootic situation on intestinal helminthiasis in the main horse-breeding areas of the Altai Republic. Coproovoscopic studies of horse feces from 8 districts (Mayminsky, Shebalinsky, Ongudaisky, Chemalsky, Ust-Koksinsky, Kosh-Agachsky and Ulagansky) were carried out for eggs of intestinal helminths by the KotelnikovKhrenov flotation method with the counting of eggs according to the VIGIS method. In total, 989 fecal samples from adult New Altai horses were examined. According to the results of coprological examinations, indicators of infection were derived: IP, %, the infection prevalence, the proportion of infected animals; mII is the arithmetic mean of the number of eggs in 1 gram of feces (egg/g f.) per one examined animal. In the horses of the Altai Mountains, pathogens of gastrointestinal helminth infections belonging to 5 suborders were recorded: Strongylata, Ascaridata, Anoplocephalata, Oxyurata and Rabditata. To a greater extent, the horses were infected with gastrointestinal Strongylata with an IP level of 76.2% and an egg count of 293 specimens in a gram of feces, and to a lesser extent with Parascarids (IP 11.2%) and Anoplocephalida (IP 12.3%). The animals in the Chemalsky District were most intensively infected with helminths (IP 96.3%), significantly lower than the horses in the Kosh-Agachsky District (IP 62.3%).
{"title":"EPIZOOTIC SITUATION ON THE MAIN GASTROINTESTINAL HELMINTH INFECTIONS OF HORSES IN THE ALTAI REPUBLIC","authors":"V. A. Marchenko, E. A. Efremova, M. A. Smertina","doi":"10.31016/978-5-6048555-6-0.2023.24.290-294","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.290-294","url":null,"abstract":"One of the problems of herd horse breeding of the Altai Mountains is significant \u0000spread of helminth infections. The purpose of this research is to characterize the \u0000epizootic situation on intestinal helminthiasis in the main horse-breeding areas \u0000of the Altai Republic. Coproovoscopic studies of horse feces from 8 districts \u0000(Mayminsky, Shebalinsky, Ongudaisky, Chemalsky, Ust-Koksinsky, Kosh-Agachsky \u0000and Ulagansky) were carried out for eggs of intestinal helminths by the KotelnikovKhrenov flotation method with the counting of eggs according to the VIGIS method. \u0000In total, 989 fecal samples from adult New Altai horses were examined. According to \u0000the results of coprological examinations, indicators of infection were derived: IP, %, \u0000the infection prevalence, the proportion of infected animals; mII is the arithmetic \u0000mean of the number of eggs in 1 gram of feces (egg/g f.) per one examined animal. In \u0000the horses of the Altai Mountains, pathogens of gastrointestinal helminth infections belonging to 5 suborders were recorded: Strongylata, Ascaridata, Anoplocephalata, \u0000Oxyurata and Rabditata. To a greater extent, the horses were infected with \u0000gastrointestinal Strongylata with an IP level of 76.2% and an egg count of 293 \u0000specimens in a gram of feces, and to a lesser extent with Parascarids (IP 11.2%) \u0000and Anoplocephalida (IP 12.3%). The animals in the Chemalsky District were most \u0000intensively infected with helminths (IP 96.3%), significantly lower than the horses \u0000in the Kosh-Agachsky District (IP 62.3%).","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83850218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.138-141
F. Vasilevich, S. A. Shemyakova, N. Esaulova
The paper describes the structure of the Museum of Parasitology located on the basis of the Department of Parasitology and Veterinary and Sanitary Expertise of the Federal State Budgetary Educational Institution of Higher Education "Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after K. I. Skryabin", which was organized by Konstantin Ivanovich Skryabin. The first gross specimens were provided by him from his own collection. Later, together with his students, K. I. Skryabin replenished the collection with helminths brought by them from numerous expeditions to various regions of the Soviet Union. In 2017, the Museum acquired a new "face", major repairs were made, and thematic stands were allocated: helminthiasis of horses, cattle and small cattle, pigs, fish, birds, dogs and cats. There are exhibits showing ticks and insects that parasitize animals. Eighty zoonotic helminth infections are allocated to a separate stand, of which 26 are caused by trematodes, 17 by cestodes, 30 by nematodes, 3 by Acanthocephali, and 4 by pentastomes. A separate part of the Museum's permanent exhibition is a section dedicated to Academician K. I. Skryabin which presents original photographs, albums, personal belongings of the Academician, letters, postcards addressed to K. I. Skryabin, lectures and dissertations of the Academician, as well as technical means by which the material was studied (microscopes, magnifying glasses), and numerous works of K. I. Skryabin.
本文描述了寄生虫学博物馆的结构，该博物馆位于联邦国家预算高等教育机构“莫斯科国立兽医和生物技术学院-以K. I. Skryabin命名的MBA”的寄生虫学和兽医卫生专业部门的基础上，该学院由康斯坦丁·伊万诺维奇·Skryabin组织。第一批粗大标本是他从自己的收藏中提供的。后来，K. I. Skryabin和他的学生一起，用他们从苏联各个地区的多次探险中带来的蠕虫补充了这些收藏。2017年，博物馆获得了新的“面孔”，进行了重大修复，并分配了主题展台:马、牛和小牛、猪、鱼、鸟、狗和猫的蠕虫病。有一些展品展示了寄生在动物身上的蜱虫和昆虫。80个人畜共患蠕虫感染被分配到一个单独的林分，其中26个由吸虫引起，17个由绦虫引起，30个由线虫引起，3个由棘头虫引起，4个由五形虫引起。博物馆常设展览的一个单独部分是专门为K. I. Skryabin院士而设的部分，其中展示了院士的原始照片，相册，个人物品，信件，寄给K. I. Skryabin的明信片，院士的讲座和论文，以及研究材料的技术手段(显微镜，放大镜)，以及K. I. Skryabin的许多作品。
{"title":"MUSEUM OF THE DEPARTMENT OF PARASITOLOGY AND VETERINARY AND SANITARY EXPERTISE NAMED AFTER K. I. SKRYABIN","authors":"F. Vasilevich, S. A. Shemyakova, N. Esaulova","doi":"10.31016/978-5-6048555-6-0.2023.24.138-141","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.138-141","url":null,"abstract":"The paper describes the structure of the Museum of Parasitology located on the \u0000basis of the Department of Parasitology and Veterinary and Sanitary Expertise of \u0000the Federal State Budgetary Educational Institution of Higher Education \"Moscow \u0000State Academy of Veterinary Medicine and Biotechnology – MBA named after \u0000K. I. Skryabin\", which was organized by Konstantin Ivanovich Skryabin. The first \u0000gross specimens were provided by him from his own collection. Later, together with \u0000his students, K. I. Skryabin replenished the collection with helminths brought by \u0000them from numerous expeditions to various regions of the Soviet Union. In 2017, \u0000the Museum acquired a new \"face\", major repairs were made, and thematic stands \u0000were allocated: helminthiasis of horses, cattle and small cattle, pigs, fish, birds, dogs \u0000and cats. There are exhibits showing ticks and insects that parasitize animals. Eighty zoonotic helminth infections are allocated to a separate stand, of which 26 are \u0000caused by trematodes, 17 by cestodes, 30 by nematodes, 3 by Acanthocephali, and 4 \u0000by pentastomes. A separate part of the Museum's permanent exhibition is a section \u0000dedicated to Academician K. I. Skryabin which presents original photographs, \u0000albums, personal belongings of the Academician, letters, postcards addressed to \u0000K. I. Skryabin, lectures and dissertations of the Academician, as well as technical \u0000means by which the material was studied (microscopes, magnifying glasses), and \u0000numerous works of K. I. Skryabin.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88335300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.470-474
R. Timerbaeva, A. R. Shageeva, G. Frolov
The epizootic situation was studied on gastrointestinal strongylatosis of horses in Sosna, LLC of the Baltasinsky District, the Republic of Tatarstan. The study objects were horses of different age groups, breeds, and sexes, spontaneously infected by nematodes. Coproscopic studies were carried out by the modified helminthovoscopic Kotelnikov-Khrenov method with ammonium nitrate and the helmintholarvoscopic Shilnikov method. The generic assignment of pathogens of gastrointestinal strongylatosis of horses was determined by cultivating nematode larvae belonging to the Strongylata suborder. As a result of the coproscopic studies, it was found that horses of different age groups were invaded by pathogens of gastrointestinal strongylatosis. In December 2021, 30 horses were tested for helminthiasis. The results of the studies showed that 20 out of 30 animals had gastrointestinal Strongylata infections with 66.7% extense-invasiveness, and the intense-invasiveness of 1 to 37 egg specimens in the field of view. The remaining 10 horses were free of helminths. The studies indicate that the examination of the horses in Sosna, LLC detected intestinal nematodiasis the causative agents of which were nematodes of the Strongylata suborder, the genus Triсhonema. Thus, widespread parasitic diseases of horses on the farm were gastrointestinal strongylatosis, in particular trichonematosis.
{"title":"GASTROINTESTINAL STRONGYLATOSIS OF HORSES","authors":"R. Timerbaeva, A. R. Shageeva, G. Frolov","doi":"10.31016/978-5-6048555-6-0.2023.24.470-474","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.470-474","url":null,"abstract":"The epizootic situation was studied on gastrointestinal strongylatosis of horses in \u0000Sosna, LLC of the Baltasinsky District, the Republic of Tatarstan. The study objects \u0000were horses of different age groups, breeds, and sexes, spontaneously infected by \u0000nematodes. Coproscopic studies were carried out by the modified helminthovoscopic \u0000Kotelnikov-Khrenov method with ammonium nitrate and the helmintholarvoscopic \u0000Shilnikov method. The generic assignment of pathogens of gastrointestinal \u0000strongylatosis of horses was determined by cultivating nematode larvae belonging to \u0000the Strongylata suborder. As a result of the coproscopic studies, it was found that horses \u0000of different age groups were invaded by pathogens of gastrointestinal strongylatosis. \u0000In December 2021, 30 horses were tested for helminthiasis. The results of the studies \u0000showed that 20 out of 30 animals had gastrointestinal Strongylata infections with \u000066.7% extense-invasiveness, and the intense-invasiveness of 1 to 37 egg specimens in the field of view. The remaining 10 horses were free of helminths. The studies indicate \u0000that the examination of the horses in Sosna, LLC detected intestinal nematodiasis \u0000the causative agents of which were nematodes of the Strongylata suborder, the \u0000genus Triсhonema. Thus, widespread parasitic diseases of horses on the farm were \u0000gastrointestinal strongylatosis, in particular trichonematosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87188339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.420-425
R. Safiullin, S. Shibitov, R. Safiullin
The study of the epizootic situation on the contamination of environmental objects with invasive elements on the livestock farm of the Moscow Region showed its contamination with parasitic protozoa oocysts and cysts, and helminth eggs and larvae which was directly affected by infection of the livestock with the above parasites and the quality of therapeutic and prophylactic and veterinary and sanitary measures. The objects of the external environment most contaminated with invasive elements of parasitic protozoa and helminths were the floor of stalls and passages in the premises for keeping animals. The stall walls and animal feeders were less contaminated with parasitic protozoa oocysts and cysts or helminth eggs or larvae. It should be noted that, the epizootic process for parasitic protozoa: Buxtonella, Cryptosporidium, Eimeria and Strongylata of the digestive tract, was permanent on the examined livestock farm of the Moscow Region, since all of its components were in place: the source of invasion was infected animals; transmission factors were environmental objects contaminated with invasive elements and susceptible animals, especially young animals. An analysis was performed of the plan of preventive antiepizootic measures of this farm in relation to parasitic diseases in terms of diagnostic tests for parasitosis, and therapeutic-and-prophylactic and veterinary-sanitary treatments.
{"title":"EPIZOOTIC SITUATION ON THE CONTAMINATION OF ENVIRONMENTAL OBJECTS WITH INVASIVE ELEMENTS ON THE CATTLE FARM OF THE MOSCOW REGION","authors":"R. Safiullin, S. Shibitov, R. Safiullin","doi":"10.31016/978-5-6048555-6-0.2023.24.420-425","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.420-425","url":null,"abstract":"The study of the epizootic situation on the contamination of environmental objects \u0000with invasive elements on the livestock farm of the Moscow Region showed its \u0000contamination with parasitic protozoa oocysts and cysts, and helminth eggs and \u0000larvae which was directly affected by infection of the livestock with the above \u0000parasites and the quality of therapeutic and prophylactic and veterinary and sanitary \u0000measures. The objects of the external environment most contaminated with invasive \u0000elements of parasitic protozoa and helminths were the floor of stalls and passages \u0000in the premises for keeping animals. The stall walls and animal feeders were less \u0000contaminated with parasitic protozoa oocysts and cysts or helminth eggs or larvae. \u0000It should be noted that, the epizootic process for parasitic protozoa: Buxtonella, \u0000Cryptosporidium, Eimeria and Strongylata of the digestive tract, was permanent \u0000on the examined livestock farm of the Moscow Region, since all of its components \u0000were in place: the source of invasion was infected animals; transmission factors were \u0000environmental objects contaminated with invasive elements and susceptible animals, especially young animals. An analysis was performed of the plan of preventive antiepizootic measures of this farm in relation to parasitic diseases in terms of diagnostic \u0000tests for parasitosis, and therapeutic-and-prophylactic and veterinary-sanitary \u0000treatments.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90551760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.386-390
B. Romashov, N. Romashova
Currently in the Central Black Earth Region, an increase in cases of infection of wild and domestic carnivores with the trematode Alaria alata (Trematoda, Strigeidida) has been recorded. Helminthological materials were collected in the Voronezh Nature Reserve and in adjacent territories (Voronezh and Lipetsk Regions) for more than 20 years (2000–2022). More than 100 species of carnivorous mammals of the Canidae family were studied. A. alata maritae were recorded in four predator species: the wolf, fox, raccoon dog, and the domestic dog. The total infection rate in 4 Canidae species was 78.3%. The highest infection prevalence was registered in the wolf, 97.0%, and the raccoon dog, 100%, and slightly lower in the fox, 81.8%. For the domestic dog, the incidence of Alaria species was found at the level of 34.4%. In the study area, the fox was the most numerous predator. The fox had significant infection prevalence values and the highest relative abundance of maritae (infection intensity and abundance index). A fairly large sample of foxes was studied in adjacent territories that were mainly agricultural lands. Relatively high levels of infection were detected: the IP 75.9%; the II 162.0; and the AI 122.9. The fox plays the leading role in the circulation of alariosis in the Central Black Earth Region.
{"title":"DEFINITIVE HOSTS OF ALARIA ALATA (TREMATODA, STRIGEIDIDA) IN NATURAL CONDITIONS OF THE CENTRAL BLACK EARTH REGION (VORONEZH AND LIPETSK REGIONS)","authors":"B. Romashov, N. Romashova","doi":"10.31016/978-5-6048555-6-0.2023.24.386-390","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.386-390","url":null,"abstract":"Currently in the Central Black Earth Region, an increase in cases of infection of wild \u0000and domestic carnivores with the trematode Alaria alata (Trematoda, Strigeidida) \u0000has been recorded. Helminthological materials were collected in the Voronezh \u0000Nature Reserve and in adjacent territories (Voronezh and Lipetsk Regions) for \u0000more than 20 years (2000–2022). More than 100 species of carnivorous mammals \u0000of the Canidae family were studied. A. alata maritae were recorded in four predator \u0000species: the wolf, fox, raccoon dog, and the domestic dog. The total infection rate in \u00004 Canidae species was 78.3%. The highest infection prevalence was registered in the \u0000wolf, 97.0%, and the raccoon dog, 100%, and slightly lower in the fox, 81.8%. For \u0000the domestic dog, the incidence of Alaria species was found at the level of 34.4%. \u0000In the study area, the fox was the most numerous predator. The fox had significant \u0000infection prevalence values and the highest relative abundance of maritae (infection \u0000intensity and abundance index). A fairly large sample of foxes was studied in adjacent \u0000territories that were mainly agricultural lands. Relatively high levels of infection were \u0000detected: the IP 75.9%; the II 162.0; and the AI 122.9. The fox plays the leading role \u0000in the circulation of alariosis in the Central Black Earth Region.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90613980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.414-419
R. Safiullin, E. Chalysheva
In our country, in recent years, much attention has been paid to the development of poultry meat production, especially turkey breeding. In the conditions of industrial turkey breeding, when a large number of poultry is kept in a limited area, there is a high risk of parasitic diseases, one of which is eimeriosis. Knowledge of the species composition of Eimeria on a particular poultry farm is of great practical importance for the reasonable development of effective methods to control invasion and to monitor Eimeria resistance to the drugs used. Eimeria species were identified after the end of sporulation. To assess the course of sporulation of Eimeria oocysts during their cultivation, at least 500 oocysts were examined from each Petri dish every six hours under a high magnification microscope (x400) paying special attention to their morphology. When examining and studying litter samples 24 hours after they were put on cultivation, sporulated Eimeria oocysts of turkeys were detected in all six dishes in 37.8% to 60.6% of those examined, and the average rate was 51.6%. At 48 hours after the start of cultivation, the average Eimeria sporulation rate was 83.4%. The results of species identification of Eimeria oocysts showed that the following Eimeria species were found in young turkeys on the poultry farm of the Tula Region: Eimeria meleagrimitis (60.0%), E. gallopavonis (25.0%), E. meleagridis (10.0%), and E. adenoides (5.0%).
{"title":"CULTURE OF EIMERIA SPP. OOCYSTS OF TURKEY POULTS AND THEIR SPECIES IDENTIFICATION","authors":"R. Safiullin, E. Chalysheva","doi":"10.31016/978-5-6048555-6-0.2023.24.414-419","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.414-419","url":null,"abstract":"In our country, in recent years, much attention has been paid to the development of \u0000poultry meat production, especially turkey breeding. In the conditions of industrial \u0000turkey breeding, when a large number of poultry is kept in a limited area, there is a \u0000high risk of parasitic diseases, one of which is eimeriosis. Knowledge of the species \u0000composition of Eimeria on a particular poultry farm is of great practical importance \u0000for the reasonable development of effective methods to control invasion and to \u0000monitor Eimeria resistance to the drugs used. Eimeria species were identified after \u0000the end of sporulation. To assess the course of sporulation of Eimeria oocysts during \u0000their cultivation, at least 500 oocysts were examined from each Petri dish every six \u0000hours under a high magnification microscope (x400) paying special attention to \u0000their morphology. When examining and studying litter samples 24 hours after they \u0000were put on cultivation, sporulated Eimeria oocysts of turkeys were detected in all six \u0000dishes in 37.8% to 60.6% of those examined, and the average rate was 51.6%. At 48 \u0000hours after the start of cultivation, the average Eimeria sporulation rate was 83.4%. \u0000The results of species identification of Eimeria oocysts showed that the following \u0000Eimeria species were found in young turkeys on the poultry farm of the Tula Region: \u0000Eimeria meleagrimitis (60.0%), E. gallopavonis (25.0%), E. meleagridis (10.0%), and \u0000E. adenoides (5.0%).","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"09 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86022187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.532-537
I. D. Shelyakin, S. N. Semenov, L. V. Cheskidova
When dogs are invaded with Babesia, metabolic and detoxification processes are disrupted. In this regard, the therapeutic regimen should include drugs that contribute to the normalization of metabolism, and maintenance of liver and kidney function. The purpose of our research was to study the effects of Аminotone on the hematological and biochemical status of dogs during the treatment of babesiosis. For this purpose, Forticarb was administered as an etiological agent to the animals of the control group, and Аminotone was additionally administered to the experimental dogs. Before the experiment and two weeks after the beginning of the treatment, blood was taken from the dogs for hematological and biochemical studies. It was found that the control and experimental animals showed a decrease in leukocytes, ESR, ALT and AST activity, urea, and creatinine and bilirubin concentrations after the recovery. At the same time, an increase in erythrocytes, hematocrit, mean corpuscular volume and hemoglobin was recorded. Аminotone provided more intensive changes in the dogs’ biochemical and hematological status: reduced inflammatory reaction, toxic load on the liver and kidneys, and stimulated hematopoiesis, versus the control. Thus, the drug can be recommended for the combined therapy of canine babesiosis.
{"title":"THE STUDY OF EFFECTS OF АMINOTONE ON HEMATOLOGICAL AND BIOCHEMICAL STATUS OF DOGS IN BABESIOSIS THERAPY","authors":"I. D. Shelyakin, S. N. Semenov, L. V. Cheskidova","doi":"10.31016/978-5-6048555-6-0.2023.24.532-537","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.532-537","url":null,"abstract":"When dogs are invaded with Babesia, metabolic and detoxification processes \u0000are disrupted. In this regard, the therapeutic regimen should include drugs that \u0000contribute to the normalization of metabolism, and maintenance of liver and kidney \u0000function. The purpose of our research was to study the effects of Аminotone on the \u0000hematological and biochemical status of dogs during the treatment of babesiosis. For \u0000this purpose, Forticarb was administered as an etiological agent to the animals of the \u0000control group, and Аminotone was additionally administered to the experimental \u0000dogs. Before the experiment and two weeks after the beginning of the treatment, blood \u0000was taken from the dogs for hematological and biochemical studies. It was found that \u0000the control and experimental animals showed a decrease in leukocytes, ESR, ALT and AST activity, urea, and creatinine and bilirubin concentrations after the recovery. \u0000At the same time, an increase in erythrocytes, hematocrit, mean corpuscular volume \u0000and hemoglobin was recorded. Аminotone provided more intensive changes in the \u0000dogs’ biochemical and hematological status: reduced inflammatory reaction, toxic \u0000load on the liver and kidneys, and stimulated hematopoiesis, versus the control. \u0000Thus, the drug can be recommended for the combined therapy of canine babesiosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73511074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.268-274
O. E. Mazur, I. Kutyrev, T. Sidorova, L. Sukhanova
New data were presented for the first time on the intestinal transcriptome of the intestines (posterior section) of the Baikal omul Coregonus migratorius Georgi, 1775 (Salmoniformes: Coregonidae) belonging to the deep water bottom morphological group (Lake Baikal) infected with parasites of various systematic groups: cestodes Dibothriocephalus dendriticus, Proteocephalus longicollis, Eubothrium crassum, and nematode Contracaecum osculatum baicalensis. Sequencing of the transcriptomics libraries was performed on an Illumina NextSeq550 sequencer using the NextSeq® 550 High Output Kit v2. Based on the obtained data, de-novo transcriptome assembly was performed. Conserved domains and their associated Gene Ontology annotations were predicted using Blast2Go. As a result, it was found that in the posterior section of the intestine enriched with lymphoid tissue (GALT), expression of functional proteins was observed that were primarily associated with enzymatic activity, with the development of specialized tissues, with cellular, metabolic and secretory processes. It should be noted that a significant proportion of gene ontology terms is associated with the functioning of the immune system, and with the cellular response to stress, which, under conditions of sensitization by the metabolic end products of helminths and their traumatic and antigenic effects, is quite understandable. The identified transcriptome may provide new information for understanding the functions of lymphoid organs in salmonids with parasitosis.
{"title":"TRANSCRIPTOME ANALYSIS OF INTESTINES OF THE BAIKAL OMUL (LAKE BAIKAL, EASTERN SIBERIA)","authors":"O. E. Mazur, I. Kutyrev, T. Sidorova, L. Sukhanova","doi":"10.31016/978-5-6048555-6-0.2023.24.268-274","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.268-274","url":null,"abstract":"New data were presented for the first time on the intestinal transcriptome of the \u0000intestines (posterior section) of the Baikal omul Coregonus migratorius Georgi, 1775 \u0000(Salmoniformes: Coregonidae) belonging to the deep water bottom morphological \u0000group (Lake Baikal) infected with parasites of various systematic groups: cestodes \u0000Dibothriocephalus dendriticus, Proteocephalus longicollis, Eubothrium crassum, and \u0000nematode Contracaecum osculatum baicalensis. Sequencing of the transcriptomics \u0000libraries was performed on an Illumina NextSeq550 sequencer using the NextSeq® \u0000550 High Output Kit v2. Based on the obtained data, de-novo transcriptome assembly was performed. Conserved domains and their associated Gene Ontology \u0000annotations were predicted using Blast2Go. As a result, it was found that in the posterior section of the intestine enriched with lymphoid tissue (GALT), expression \u0000of functional proteins was observed that were primarily associated with enzymatic \u0000activity, with the development of specialized tissues, with cellular, metabolic and \u0000secretory processes. It should be noted that a significant proportion of gene ontology \u0000terms is associated with the functioning of the immune system, and with the cellular \u0000response to stress, which, under conditions of sensitization by the metabolic end \u0000products of helminths and their traumatic and antigenic effects, is quite understandable. The identified transcriptome may provide new information for understanding \u0000the functions of lymphoid organs in salmonids with parasitosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88983221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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