Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.62-66
U. V. Bagaeva, A. R. Salbieva
The Republic of North Ossetia Alania remains contaminated by a number of trematode infections including dicroceliasis. The infection rate of cattle is on average 53% in the Republic. Five hundred twenty-six specimens of terrestrial mollusks were collected by the route method in various biocenoses of the foothill zone located at an altitude of 530 – 850 m above sea level. Fourteen species were identified. The dissections revealed that dicroceliasis foci were maintained and function with the involvement of two species of terrestrial mollusks in stock routes and pastures of the studied zone. According to the laboratory studies, infection of the mollusk Helicella derbentina (Kryn.) with the trematode larvae was 5.1%. Five of 98 dissected specimens were infected. The infection rate in Chondrula tridens (Mull.) was 4.6%. During the helminthological study of 87 specimens of this species, dicrocelia larvae were found in 4. According to preliminary data, such species of the studied terrestrial mollusks as Succinea pfeifferi (Rossm.), Cochlicopa lubrica (Műll.), Euomphalia ravergieri (Fer.), Euomphalia selecta (Klika), and Fruticocampylaea narzanensis (Kryn.) identified in stock routes and pastures of the foothill zone do not participate in the development cycle of the trematode Dicrocoelium lanceatum. Further research is required.
{"title":"SIGNIFICANCE OF TERRESTRIAL MOLLUSKS IN THE FORMATION OF DICROCELIASIS FOCI IN THE FOOTHILL ZONE OF THE REPUBLIC OF NORTH OSSETIA-ALANIA","authors":"U. V. Bagaeva, A. R. Salbieva","doi":"10.31016/978-5-6048555-6-0.2023.24.62-66","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.62-66","url":null,"abstract":"The Republic of North Ossetia Alania remains contaminated by a number of \u0000trematode infections including dicroceliasis. The infection rate of cattle is on average \u000053% in the Republic. Five hundred twenty-six specimens of terrestrial mollusks were \u0000collected by the route method in various biocenoses of the foothill zone located at \u0000an altitude of 530 – 850 m above sea level. Fourteen species were identified. The \u0000dissections revealed that dicroceliasis foci were maintained and function with the \u0000involvement of two species of terrestrial mollusks in stock routes and pastures of \u0000the studied zone. According to the laboratory studies, infection of the mollusk \u0000Helicella derbentina (Kryn.) with the trematode larvae was 5.1%. Five of 98 dissected \u0000specimens were infected. The infection rate in Chondrula tridens (Mull.) was 4.6%. \u0000During the helminthological study of 87 specimens of this species, dicrocelia larvae \u0000were found in 4. According to preliminary data, such species of the studied terrestrial \u0000mollusks as Succinea pfeifferi (Rossm.), Cochlicopa lubrica (Műll.), Euomphalia \u0000ravergieri (Fer.), Euomphalia selecta (Klika), and Fruticocampylaea narzanensis \u0000(Kryn.) identified in stock routes and pastures of the foothill zone do not participate \u0000in the development cycle of the trematode Dicrocoelium lanceatum. Further research \u0000is required.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86618600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.359-364
V. S. Pashaev, B. Y. Shogenov, A. M. Bittirov
The article is devoted to the study of regional epizootology of drepanidotaeniosis of geese of different age groups in the Kabardino-Balkar Republic (the KBR). The distribution of drepanidotaeniosis in Kuban geese was studied in 2017–2021 on the basis of complete helminthological dissections of the small intestine of 300 slaughtered geese of different age groups. It was found that drepanidotaeniosis of geese has a mosaic distribution with the coverage of the most part of outdoor young birds at the age of 2 weeks to 4 months. In the study of the Kuban geese, it was found that the prevalence of invasion (IP) was characterized by high and moderate values. The average IP of drepanidotaeniosis in the geese was 17.33±1.25%. In the Kuban geese in the context of natural and climatic zones of the KBR, drepanidotaeniosis manifests itself with threatening values of invasion intensity (II). Drepanidotaeniosis in the plain zone was found with the II of 1–3 specimens/bird; in the foothill zone, 2–5 specimens/bird; and in the mountainous zone, 1–2 specimens/bird (average 2.33±0.28 specimens/bird). Indicators of the IP and II of drepanidotaeniosis in the Kuban geese in the KBR tended to increase in goslings aged 20 to 120 days. The opposite was observed in subsequent age groups.
{"title":"PECULIARITIES OF REGIONAL EPIZOOTOLOGY OF DREPANIDOTAENIOSIS OF KUBAN GEESE IN THE KABARDINO-BALKAR REPUBLIC","authors":"V. S. Pashaev, B. Y. Shogenov, A. M. Bittirov","doi":"10.31016/978-5-6048555-6-0.2023.24.359-364","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.359-364","url":null,"abstract":"The article is devoted to the study of regional epizootology of drepanidotaeniosis of \u0000geese of different age groups in the Kabardino-Balkar Republic (the KBR). The \u0000distribution of drepanidotaeniosis in Kuban geese was studied in 2017–2021 on the \u0000basis of complete helminthological dissections of the small intestine of 300 slaughtered \u0000geese of different age groups. It was found that drepanidotaeniosis of geese has a mosaic \u0000distribution with the coverage of the most part of outdoor young birds at the age of 2 \u0000weeks to 4 months. In the study of the Kuban geese, it was found that the prevalence \u0000of invasion (IP) was characterized by high and moderate values. The average IP of \u0000drepanidotaeniosis in the geese was 17.33±1.25%. In the Kuban geese in the context \u0000of natural and climatic zones of the KBR, drepanidotaeniosis manifests itself with \u0000threatening values of invasion intensity (II). Drepanidotaeniosis in the plain zone was \u0000found with the II of 1–3 specimens/bird; in the foothill zone, 2–5 specimens/bird; and \u0000in the mountainous zone, 1–2 specimens/bird (average 2.33±0.28 specimens/bird). Indicators of the IP and II of drepanidotaeniosis in the Kuban geese in the KBR tended \u0000to increase in goslings aged 20 to 120 days. The opposite was observed in subsequent \u0000age groups.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80407557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.95-99
S. A. Bolyahina, E. A. Efremova
A comparison was done for changes in the morphological composition of blood in hens experimentally infected with T. pseudospiralis and T. spiralis larvae at a dose of 2 larvae/g m.a., intragastrically. It was established that trichinellosis in the hens due to the parasitism of a non-encapsulated Trichinella species caused regenerative hypochromic anemia. Indicators of the trichinellosis process are granulocytic series cells, heterophils. The onset of the body's reaction to infection was detected on day 4 after invasion and was expressed by mild neutrophilia. Then an increase was observed in the content of heterophils in the blood of the birds with a maximum on day 29, which corresponds to the migration and muscular stage of the trichinellosis process. Subsequently, there is a relative and absolute quantitative decrease in these groups of cells in the bloodstream of the poultry, however, these indicators are higher during the entire observation period (61 days) than in the hens of the control group. Lymphocytes in the blood samples from the experimental hens have an absolute increase on day 50 from the infection, which can be characterized as an immunological reaction of the hens in the invasion. The dynamics of the level of eosinophils and basophils circulating in the blood of the poultry is due to the stages of the trichinellosis process and confirms the predominance of an allergic reaction in the nature of the disease. However, more pronounced quantitative changes in hematological parameters, especially on the part of heterophils and eosinophils were recorded in the poultry infected with a non-encapsulated Trichinella species.
{"title":"COMPARISON OF MORPHOLOGICAL CHANGES IN THE BLOOD OF HENS DURING EXPERIMENTAL INFECTION WITH T. SPIRALIS AND T. PSEUDOSPIRALIS","authors":"S. A. Bolyahina, E. A. Efremova","doi":"10.31016/978-5-6048555-6-0.2023.24.95-99","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.95-99","url":null,"abstract":"A comparison was done for changes in the morphological composition of blood in \u0000hens experimentally infected with T. pseudospiralis and T. spiralis larvae at a dose \u0000of 2 larvae/g m.a., intragastrically. It was established that trichinellosis in the hens \u0000due to the parasitism of a non-encapsulated Trichinella species caused regenerative \u0000hypochromic anemia. Indicators of the trichinellosis process are granulocytic series \u0000cells, heterophils. The onset of the body's reaction to infection was detected on \u0000day 4 after invasion and was expressed by mild neutrophilia. Then an increase was \u0000observed in the content of heterophils in the blood of the birds with a maximum on \u0000day 29, which corresponds to the migration and muscular stage of the trichinellosis \u0000process. Subsequently, there is a relative and absolute quantitative decrease in \u0000these groups of cells in the bloodstream of the poultry, however, these indicators \u0000are higher during the entire observation period (61 days) than in the hens of the \u0000control group. Lymphocytes in the blood samples from the experimental hens have \u0000an absolute increase on day 50 from the infection, which can be characterized as an \u0000immunological reaction of the hens in the invasion. The dynamics of the level of \u0000eosinophils and basophils circulating in the blood of the poultry is due to the stages \u0000of the trichinellosis process and confirms the predominance of an allergic reaction in the nature of the disease. However, more pronounced quantitative changes in \u0000hematological parameters, especially on the part of heterophils and eosinophils were \u0000recorded in the poultry infected with a non-encapsulated Trichinella species.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80476647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.381-385
B. Romashov, A. Bakhtina, A. Golubtsov
Cyprinids as the second intermediate host are a source of infection of the definitive host with zoonotic trematode infections. Currently in some regions of European Russia, opisthorchiasis is constantly recorded in humans. This relates to the Lipetsk Region, where the species diversity and distribution of opisthorchides, ecological biological and epizootological aspects of the circulation of opisthorchidosis remain unstudied. The materials were collected in 2021-2022 in the south-eastern part of the Lipetsk Region on the Usman River. We studied 179 specimens of 4 Cyprinid species: the roach (97 specimens), the bleak (41 specimens), the silver bream (21 specimens) and the rudd (20 specimens). The highest infection rates were observed for opisthorchide metacercariae (Opisthorchis felineus, Pseudamphistomum truncatum, Metorchis bilis and Metorchis xanthosomus) with the infection prevalence (IP) of 78.2%; the second position was occupied by Posthodiplostomum cuticola with the IP 63.7%; they were followed by Paracoenogonimus ovatus with similar parameters with the IP 21.8% and Metorchis xanthosomus with the IP 19.6%. According to the results of our research, 6 species of trematode metacercariae including 3 opisthorchide species (O. felineus, P. truncatum, M. bilis) of epidemiological significance were recorded for the first time in cyprinid fish in the Lipetsk Region. For all metacercariae species, the current parameters of infection in cyprinids were revealed.
{"title":"CURRENT DATA ON THE SPECIES DIVERSITY OF TREMATODE METACERCARIA IN CYPRINIDS IN THE LIPETSK REGION","authors":"B. Romashov, A. Bakhtina, A. Golubtsov","doi":"10.31016/978-5-6048555-6-0.2023.24.381-385","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.381-385","url":null,"abstract":"Cyprinids as the second intermediate host are a source of infection of the definitive host \u0000with zoonotic trematode infections. Currently in some regions of European Russia, \u0000opisthorchiasis is constantly recorded in humans. This relates to the Lipetsk Region, \u0000where the species diversity and distribution of opisthorchides, ecological biological \u0000and epizootological aspects of the circulation of opisthorchidosis remain unstudied. \u0000The materials were collected in 2021-2022 in the south-eastern part of the Lipetsk \u0000Region on the Usman River. We studied 179 specimens of 4 Cyprinid species: the \u0000roach (97 specimens), the bleak (41 specimens), the silver bream (21 specimens) and the rudd (20 specimens). The highest infection rates were observed for opisthorchide \u0000metacercariae (Opisthorchis felineus, Pseudamphistomum truncatum, Metorchis bilis \u0000and Metorchis xanthosomus) with the infection prevalence (IP) of 78.2%; the second \u0000position was occupied by Posthodiplostomum cuticola with the IP 63.7%; they were \u0000followed by Paracoenogonimus ovatus with similar parameters with the IP 21.8% and \u0000Metorchis xanthosomus with the IP 19.6%. According to the results of our research, 6 \u0000species of trematode metacercariae including 3 opisthorchide species (O. felineus, P. \u0000truncatum, M. bilis) of epidemiological significance were recorded for the first time \u0000in cyprinid fish in the Lipetsk Region. For all metacercariae species, the current \u0000parameters of infection in cyprinids were revealed.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89693610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.189-193
V. Ivanov, T. Sivkova
Dirofilaria repens infection is widespread in animals in the Russian Federation; however, morphological changes in this disease are not described sufficiently, and carcinogenic effects of helminths to the host organism are not established. We have studied blood changes in mice after a single subcutaneous injection of somatic extract from mature Dirofilaria. The mice were euthanized after 12 hours; blood samples were taken for а complete blood count (CBC) by an automated hematology analyzer and for slides preparation to count a white blood cell differential. In the control group, all the studied parameters were within the reference values with the exception of increased hematocrit. According to the results of the leukogram analysis in mice of the control group, slight lymphocytopenia, and an increase in the number of immature and stab neutrophils were recorded. After a single injection of the D. repens extract, two specimens from the experimental group showed only increased hematocrit, while other animals had leukocytopenia. The count of white blood cells in the experimental group detected neutrophilic leukocytosis with an increase in the number of immature, stab and segmented neutrophils. The detected changes indicate initiated nonspecific immune response.
{"title":"EFFECT OF DIROFILARIA REPENS EXTRACT ON HEMATOLOGICAL PARAMETERS OF MICE IN A SINGLE INJECTION","authors":"V. Ivanov, T. Sivkova","doi":"10.31016/978-5-6048555-6-0.2023.24.189-193","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.189-193","url":null,"abstract":"Dirofilaria repens infection is widespread in animals in the Russian Federation; \u0000however, morphological changes in this disease are not described sufficiently, and \u0000carcinogenic effects of helminths to the host organism are not established. We have \u0000studied blood changes in mice after a single subcutaneous injection of somatic \u0000extract from mature Dirofilaria. The mice were euthanized after 12 hours; blood \u0000samples were taken for а complete blood count (CBC) by an automated hematology \u0000analyzer and for slides preparation to count a white blood cell differential. In the \u0000control group, all the studied parameters were within the reference values with \u0000the exception of increased hematocrit. According to the results of the leukogram \u0000analysis in mice of the control group, slight lymphocytopenia, and an increase in \u0000the number of immature and stab neutrophils were recorded. After a single injection \u0000of the D. repens extract, two specimens from the experimental group showed only \u0000increased hematocrit, while other animals had leukocytopenia. The count of white \u0000blood cells in the experimental group detected neutrophilic leukocytosis with an \u0000increase in the number of immature, stab and segmented neutrophils. The detected \u0000changes indicate initiated nonspecific immune response.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"192 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79653012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.262-267
O. E. Mazur
New data were received on infection with Kinetoplastida of the family Trypanosomatidae in fish of various taxonomic groups in lakes in the Republic of Buryatia (Eastern Siberia). Kinetoplastids of the genus Trypanosoma recorded in the river perch, common pike, sand sculpin, Siberian loach, crucian carp, and Siberian roach were characterized by the greatest host diversity. The roach (Gusinoe Lake) is recorded for the first time for the Baikal basin as a host for hemoflagellates of this genus. Blood parasites of the genus Trypanoplasma were found in 3 fish species: the pike, perch, and sand sculpin. A clear trend was noted for the increasing infection level in the perch and pike of Gusinoe Lake over a 40-year period. The dace, Amur carp, Amur catfish, burbot, Amur sleeper, and common minnow from Gusinoe Lake were free from blood parasites Trypanosoma sp. and Trypanoplasma sp. Kinetoplastids were not found in roaches from Tsaidam, Shchuchye, Torma, Kotokelskoe and Bolshoye Kicherskoe Lakes. The analysis showed no infection with kinetoplastids in the roach, dace, Baunt whitefish, humpback whitefish, Siberian cisco, perch, burbot, ruff, and pike in the reservoirs of the Baunt Lakes system (Bolshoe Kopylyushi Lake, Maloe Kopylyushi Lake, Baunt Lake, the Lena basin), and crucian carp in reservoirs Klyukvennaya Pad, Cheremukhovoye Lake and Nikitkino Lake (Baikal basin).
{"title":"NEW DATA ON THE INFECTION OF FISH WITH HEMOFLAGELLATES (PROTOZOA: KINETOPLASTEA) IN SOME WATER BODIES OF BURYATIA (EASTERN SIBERIA)","authors":"O. E. Mazur","doi":"10.31016/978-5-6048555-6-0.2023.24.262-267","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.262-267","url":null,"abstract":"New data were received on infection with Kinetoplastida of the family Trypanosomatidae in fish of various taxonomic groups in lakes in the Republic of Buryatia (Eastern Siberia). Kinetoplastids of the genus Trypanosoma recorded in the river perch, \u0000common pike, sand sculpin, Siberian loach, crucian carp, and Siberian roach were \u0000characterized by the greatest host diversity. The roach (Gusinoe Lake) is recorded \u0000for the first time for the Baikal basin as a host for hemoflagellates of this genus. \u0000Blood parasites of the genus Trypanoplasma were found in 3 fish species: the pike, \u0000perch, and sand sculpin. A clear trend was noted for the increasing infection level \u0000in the perch and pike of Gusinoe Lake over a 40-year period. The dace, Amur carp, \u0000Amur catfish, burbot, Amur sleeper, and common minnow from Gusinoe Lake were \u0000free from blood parasites Trypanosoma sp. and Trypanoplasma sp. Kinetoplastids \u0000were not found in roaches from Tsaidam, Shchuchye, Torma, Kotokelskoe and Bolshoye Kicherskoe Lakes. The analysis showed no infection with kinetoplastids in the \u0000roach, dace, Baunt whitefish, humpback whitefish, Siberian cisco, perch, burbot, \u0000ruff, and pike in the reservoirs of the Baunt Lakes system (Bolshoe Kopylyushi Lake, \u0000Maloe Kopylyushi Lake, Baunt Lake, the Lena basin), and crucian carp in reservoirs \u0000Klyukvennaya Pad, Cheremukhovoye Lake and Nikitkino Lake (Baikal basin).","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"134 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77367191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.343-348
D. Panova, O. Panova
Parasitic diseases cause significant damage to livestock affecting the productivity and clinical condition of sheep and goats. The purpose of our research was to provide a comprehensive diagnosis of endoparasites in ruminants kept on a private livestock farm in the Moscow Region, and to develop and propose a scheme of therapeutic and preventive measures. Fifty five fecal samples were studied: 19 samples from cows (4 from calves, 15 from adults), 24 from goats (10 from goatlings, 14 from adults), 12 from sheep (all age groups were kept together). Fecal samples were taken individually from the cows and goats, and by a group method from the sheep. The samples were examined on the sampling day by the flotation method. The therapy was carried out on a group of goatlings with intense Eimeria spp. infection using toltrazuril at a dose of 15 mg/kg once daily for 3 consecutive days. The adult sheep and goats were given a combined preparation with praziquantel 15 mg and ivermectin 3 mg per 10 kg of animal weight, as a single dose. Fourteen days after helminth therapy, toltrazuril was prescribed at a dose of 15 mg/kg once a day for 2 consecutive days. Toltrazuril was administered individually, and the anthelmintic was chosen in the form of a suspension - it was given to the goats individually, and to the sheep by a group method in food. Fecal samples from the goatlings were examined on day 7 after the anticoccidial drug, and fecal samples from the adult animals were examined on day 14 after the anthelmintic therapy and on day 7 after the anticoccidial drug.
{"title":"EXPERIENCE OF SANITATION OF LIVESTOCK FARMS WITH PARASITOSIS","authors":"D. Panova, O. Panova","doi":"10.31016/978-5-6048555-6-0.2023.24.343-348","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.343-348","url":null,"abstract":"Parasitic diseases cause significant damage to livestock affecting the productivity and \u0000clinical condition of sheep and goats. The purpose of our research was to provide a \u0000comprehensive diagnosis of endoparasites in ruminants kept on a private livestock \u0000farm in the Moscow Region, and to develop and propose a scheme of therapeutic \u0000and preventive measures. Fifty five fecal samples were studied: 19 samples from cows \u0000(4 from calves, 15 from adults), 24 from goats (10 from goatlings, 14 from adults), 12 \u0000from sheep (all age groups were kept together). Fecal samples were taken individually \u0000from the cows and goats, and by a group method from the sheep. The samples were \u0000examined on the sampling day by the flotation method. The therapy was carried \u0000out on a group of goatlings with intense Eimeria spp. infection using toltrazuril at \u0000a dose of 15 mg/kg once daily for 3 consecutive days. The adult sheep and goats \u0000were given a combined preparation with praziquantel 15 mg and ivermectin 3 mg \u0000per 10 kg of animal weight, as a single dose. Fourteen days after helminth therapy, \u0000toltrazuril was prescribed at a dose of 15 mg/kg once a day for 2 consecutive days. \u0000Toltrazuril was administered individually, and the anthelmintic was chosen in the \u0000form of a suspension - it was given to the goats individually, and to the sheep by a \u0000group method in food. Fecal samples from the goatlings were examined on day 7 \u0000after the anticoccidial drug, and fecal samples from the adult animals were examined \u0000on day 14 after the anthelmintic therapy and on day 7 after the anticoccidial drug.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87420256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.46-51
O. Andreyanov, A. Uspensky, A. Postevoy
Currently, the question of the role of the domesticated cat as the definitive host of the tapeworm E. multilocularis remains controversial. The purpose of the present studies was to experimentally identify the role of the domesticated cat in the spread of alveococcosis. Rats infected with metacestodes E. multilocularis were euthanized, hydatids were dissected, passed through a meat grinder, and a protoscolex suspension was obtained. Scoleces in the Mogileva-Kotelnikov chamber were counted, their viability was assessed, and 12 domesticated cats were infected with a dose of 20,000 scoleces per animal. The animals were euthanized weekly. The cats were examined by helminthological methods. After a week of the experiment, it was found that helminths got acclimatized in the cats. In the small intestine of the adult cats, 382 and 1500 (1.9 and 7.5%) scoleces of the alveolar cestode were found, and 980 and 2500 (4.9 and 12.5%) in the kittens. At the second week of the experiment, 210 and 800 (1.0 and 4.0%) immature cestodes were recorded in the adult cats, and 1600 and 550 (8.0 and 2.7%) immature cestodes were recorded in the kittens. In the third week of the experiment, cestodes were not found in the intestines of the animals. Minimal prevalence, and intensity of the alveococcosis lesion invasion in the domesticated cat (Felis catus) shows the questionable epizootic nature of alveolar echinococcosis.
{"title":"ROLE OF DOMESTICATED CAT (FELIS CATUS) IN EPIZOOTOLOGY OF ALVEOLAR ECHINOCOCCOSIS","authors":"O. Andreyanov, A. Uspensky, A. Postevoy","doi":"10.31016/978-5-6048555-6-0.2023.24.46-51","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.46-51","url":null,"abstract":"Currently, the question of the role of the domesticated cat as the definitive host of \u0000the tapeworm E. multilocularis remains controversial. The purpose of the present \u0000studies was to experimentally identify the role of the domesticated cat in the spread \u0000of alveococcosis. Rats infected with metacestodes E. multilocularis were euthanized, \u0000hydatids were dissected, passed through a meat grinder, and a protoscolex suspension \u0000was obtained. Scoleces in the Mogileva-Kotelnikov chamber were counted, their \u0000viability was assessed, and 12 domesticated cats were infected with a dose of 20,000 \u0000scoleces per animal. The animals were euthanized weekly. The cats were examined by \u0000helminthological methods. After a week of the experiment, it was found that helminths \u0000got acclimatized in the cats. In the small intestine of the adult cats, 382 and 1500 (1.9 \u0000and 7.5%) scoleces of the alveolar cestode were found, and 980 and 2500 (4.9 and \u000012.5%) in the kittens. At the second week of the experiment, 210 and 800 (1.0 and 4.0%) \u0000immature cestodes were recorded in the adult cats, and 1600 and 550 (8.0 and 2.7%) immature cestodes were recorded in the kittens. In the third week of the experiment, \u0000cestodes were not found in the intestines of the animals. Minimal prevalence, and \u0000intensity of the alveococcosis lesion invasion in the domesticated cat (Felis catus) shows \u0000the questionable epizootic nature of alveolar echinococcosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86084097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.431-435
A. Sidelnikova
Deviations of hemogram values in distant periods of invasion in opisthorchiasis are poorly studied, they are only usually studied in case of serious complications of the disease. An experiment was carried out on sexually mature male rodents (rabbits). The invasive dose for each animal in the observation group was 50 metacercariae of Opisthorchis felineus (n=10). Clinically healthy male animals of the same age and sex (n=10) were the control group. The leukogram was studied after 30 months to assess the condition of the animals. Capillary blood smears were fixed and stained by May-Grunwald and Romanowsky-Giemsa methods and examined by light immersion microscopy manually using the meander method. Statistical data processing was performed using the Microsoft Excel application package by calculating the arithmetic mean with the standard error of the mean. Analysis of intergroup data was performed using nonparametric Mann-Whitney U-criterion for unrelated populations with non-normal distribution with p ≤ 0.05. In an intergroup comparison of the data obtained, the number of lymphocytes in the observation group was 1.35 less than the control; stab neutrophils were 5.8 less than the control; the differences were statistically significant. The number of segmented neutrophils in the observation group was 1.38 greater than the control (differences were statistically insignificant), eosinophils were 9.6 greater and basophils were 12.3 greater, and differences were statistically significant. It was found that lymphopenia, stab neutrophils, segmented neutrophils, eosinophilia and basophilia were observed in the chronic phase of infection in the rabbits after 30 months.
{"title":"CHANGES IN SOME VALUES ON THE HEMOGRAM IN THE CHRONIC PHASE OF OPISTHORCHIASIS IN THE EXPERIMENT","authors":"A. Sidelnikova","doi":"10.31016/978-5-6048555-6-0.2023.24.431-435","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.431-435","url":null,"abstract":"Deviations of hemogram values in distant periods of invasion in opisthorchiasis \u0000are poorly studied, they are only usually studied in case of serious complications \u0000of the disease. An experiment was carried out on sexually mature male rodents \u0000(rabbits). The invasive dose for each animal in the observation group was 50 \u0000metacercariae of Opisthorchis felineus (n=10). Clinically healthy male animals of \u0000the same age and sex (n=10) were the control group. The leukogram was studied \u0000after 30 months to assess the condition of the animals. Capillary blood smears \u0000were fixed and stained by May-Grunwald and Romanowsky-Giemsa methods and \u0000examined by light immersion microscopy manually using the meander method. \u0000Statistical data processing was performed using the Microsoft Excel application \u0000package by calculating the arithmetic mean with the standard error of the mean. \u0000Analysis of intergroup data was performed using nonparametric Mann-Whitney \u0000U-criterion for unrelated populations with non-normal distribution with p ≤ 0.05. \u0000In an intergroup comparison of the data obtained, the number of lymphocytes in the \u0000observation group was 1.35 less than the control; stab neutrophils were 5.8 less than \u0000the control; the differences were statistically significant. The number of segmented \u0000neutrophils in the observation group was 1.38 greater than the control (differences \u0000were statistically insignificant), eosinophils were 9.6 greater and basophils were 12.3 \u0000greater, and differences were statistically significant. It was found that lymphopenia, \u0000stab neutrophils, segmented neutrophils, eosinophilia and basophilia were observed \u0000in the chronic phase of infection in the rabbits after 30 months.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88968694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.31016/978-5-6048555-6-0.2023.24.251-255
G. V. Kuznetsov, D. E. Mitkovskii, N. Kreshchenko
Serotonin-immunopositive (IP) components were detected in the nervous system of planarians Schmidtea mediterranea using immunocytochemistry and fluorescence microscopy. Characteristics of serotonergic nervous system were detected by the immunocytochemical staining. The nervous system of the planarian S. mediterranea is represented by the arc-shaped head nerve ganglion located in the anterior region of the planarian; the inner part of the ganglion is formed by nerve cell processes; whereas neurocytons are situated on the ganglion surface. Well-stained ventral nerve cords connected by serotonin-IP commissures are extending from the ganglion caudally. A morphometric analysis of serotonergic nerve elements was performed on frozen serial sections of planarian tissues and showed that the thickness of the nerve ganglion stained by serotonin antibodies was about 110 µm, the size of serotonin neurons in the head ganglion varied from 9 to 26 µm, and the size of serotonergic neurons within the nerve plexus innervating the planarian body musculature was 7 to 13 µm. This information may be of evolutionary importance and will be useful for a comparative analysis of morphometric parameters of serotonergic neuron structures in free-living and parasitic flatworms.
{"title":"SEROTONIN NEURON STRUCTURES IN THE NERVOUS SYSTEM OF PLANARIANS SCHMIDTEA MEDITERRANEA (PLATYHELMINTHES)","authors":"G. V. Kuznetsov, D. E. Mitkovskii, N. Kreshchenko","doi":"10.31016/978-5-6048555-6-0.2023.24.251-255","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.251-255","url":null,"abstract":"Serotonin-immunopositive (IP) components were detected in the nervous system \u0000of planarians Schmidtea mediterranea using immunocytochemistry and fluorescence \u0000microscopy. Characteristics of serotonergic nervous system were detected by the \u0000immunocytochemical staining. The nervous system of the planarian S. mediterranea \u0000is represented by the arc-shaped head nerve ganglion located in the anterior region \u0000of the planarian; the inner part of the ganglion is formed by nerve cell processes; \u0000whereas neurocytons are situated on the ganglion surface. Well-stained ventral nerve \u0000cords connected by serotonin-IP commissures are extending from the ganglion \u0000caudally. A morphometric analysis of serotonergic nerve elements was performed on \u0000frozen serial sections of planarian tissues and showed that the thickness of the nerve \u0000ganglion stained by serotonin antibodies was about 110 µm, the size of serotonin \u0000neurons in the head ganglion varied from 9 to 26 µm, and the size of serotonergic \u0000neurons within the nerve plexus innervating the planarian body musculature was 7 to \u000013 µm. This information may be of evolutionary importance and will be useful for a \u0000comparative analysis of morphometric parameters of serotonergic neuron structures \u0000in free-living and parasitic flatworms.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75911258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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