The Scientific World Journal最新文献

The challenge in polystyrene disposal has caused researchers to look for urgent innovative and ecofriendly solutions for plastic degradation. Some insects have been reported to use polystyrene as their sole carbon source, and this has been linked to the presence of microbes in their guts that aid in plastic digestion. Thus, this study focuses on the molecular detection and phylogenetic analysis of the alkane-1-monooxygenase (alkB) gene in Klebsiella oxytoca strains isolated from the gut of Tenebrio molitor. The alkB gene encodes for alkane-1-monooxygenase, an enzyme involved in the oxidation of inactivated alkanes. This gene can be used as a marker to assess bacteria's ability to biodegrade polystyrene. Three bacterial strains were isolated from the guts of T. molitor mealworms and were confirmed using polymerase chain reaction (PCR) of the 16S ribosomal RNA gene. The primers used in the amplification of the 16S ribosomal RNA region were designed using NCBI, a bioinformatics tool. To detect the presence of the alkB gene in the isolated bacterial strains, a set of primers used in the amplification of this gene was manually designed from the conserved regions of the alkB nucleotide sequences of eleven bacterial species from GenBank. TCOFFE online tool was used to align the alkB sequences of the bacteria, while Jalview and ConSurf were used to view the alignment. The amplified alkB gene was then sequenced using the Sanger sequencing technique, blasted on NCBI to look for similar sequences, and a phylogenetic tree was constructed. Based on the 16S ribosomal RNA gene sequences, the isolated bacterial strains were confirmed to be Klebsiella oxytoca NBRC 102593, Klebsiella oxytoca JCM 1665, and Klebsiella oxytoca ATCC 13182. The alkB gene sequence identical to fourteen alkB gene sequences derived from Actinobacteria whole genome was detected in Klebsiella oxytoca for the first time to the best of our knowledge. The novel nucleotide sequence was published in the NCBI database under accession number OP959069. This gene sequence was found to be for the enzyme alkane-1-monooxygenase and may be one of the enzymes responsible for polystyrene degradation by the putative Klebsiella oxytoca ATCC 13182 in T. molitor.

Methods: A cross-sectional survey was conducted using a structured questionnaire involving 402 motorcyclists from four major southeastern towns, comprising 350 (86.07%) males and 52 (12.93%) females. The chi-square test was applied in bivariate analysis, and binary multivariable logistic regression was performed to determine the risk factors of road traffic crashes.

Results: This study's findings revealed that the overall reported prevalence of road traffic crashes involving motorcycle drivers over one year was 68.66%. Multivariable logistic regression analysis revealed several factors that significantly impacted road traffic crashes. These factors included driving without a valid driving license, the young age (<20) of motorcyclists, driving in rainy weather, exceeding the speed limit, per-week working hours, smoking status, motorcycle ownership, the brand of motorcycle, and not wearing a helmet while driving.

Conclusion: The study findings highlight the need for improving motorcycle safety by implementing measures such as imposing per-week work hour limits for riders, enforcing traffic regulations, and promoting helmet use among motorcycle drivers. The results of this study draw attention to the Bangladesh Road Transport Authority (BRTA) and motorcycle drivers in the country to decrease motorcycle crashes and the severity of injuries by implementing efficient guidelines and strategies for driving motorcycles. The findings of this study can assist policymakers and concerned authorities in taking the essential steps to lessen road traffic crashes among motorcyclists in Bangladesh.

Soil degradation emerges as one of the major problems in the locality of Sir in the Mandara Mountains, Far-North Cameroon. Inappropriate agricultural techniques resulting in land use change affect soil functions and seriously harm forest ecosystems. This study was conducted to analyse the current character of soils and access their degradation and their level of fertility. Twenty soil samples were taken at 15 cm depth. This includes ten in the plot under forest reserve and ten others in the plot under cultivation. Cultivation is responsible for the increase in bulk density (BD) (1.59 to 2.23 g/cm³), Mg (4.76 to 6.40 cmol·kg^-1), Ca (10.44 to 11.26 cmol·kg^-1), P (7.93 to 9.93 g/kg), and Mg/K (2.28 to 5.84) and decrease in CEC (38 0.15 to 31.46 cmol·kg^-1), OM (2.76 to 1.08%), OC (1.66 to 0.62%), total nitrogen (0.08 to 0.05%), K (4.59 to 1.15 cmol·kg^-1), Na (1.32 to 0.91 cmol·kg^-1), C/N (25.69 to 13.86), and Ca/Mg (2.32 to 1.89). This variability in physicochemical properties reflects progressive soil degradation. Cultivated soils are subject to severe degradation or potential vulnerability (SDR/Vp = 4/2) due to texture, organic carbon, soil aggregate stability, sealing index, and total nitrogen. On the other hand, soils under forest reserve are subject to severe degradation or vulnerability due to the total nitrogen and sodium absorption ratio. The soils of the study area are subject to severe and extreme potential degradation or vulnerability due to BD, respectively, under forest reserve and cultivation. Two classes of fertility were identified: class II (plots under forest reserve) having a good level of fertility, characterized by good physical properties and severe limitations in nitrogen and phosphorus and class IV (cultivated plots) with a low level of fertility due to severe limitations in organic matter, phosphorus, and poor physical characteristics. The best indicator of the good quality of the luvisols of Sir is the pH, while the bulk density is an indicator of severe to very extreme degradation or high to very high vulnerability. The application of organic and mineral amendments is essential for raising the organic matter and nitrogen and phosphorus contents in these soils.

Cabbage (Brassica oleracea var. capitata L.) holds significant agricultural and nutritional importance in Ethiopia; yet, its production faces challenges, including suboptimal nitrogen fertilizer management. The aim of this review was to review the possible effect of nitrogen fertilizer levels on the production of cabbage in Ethiopia. Nitrogen fertilization significantly influences cabbage yield and quality. Moderate to high levels of nitrogen application enhance plant growth, leaf area, head weight, and yield. However, excessive nitrogen levels can lead to adverse effects such as delayed maturity, increased susceptibility to pests and diseases, and reduced postharvest quality. In Ethiopia, small-scale farmers use different nitrogen levels for cabbage cultivation. In Ethiopia, NPSB or NPSBZN fertilizers are widely employed for the growing of various crops such as cabbage. 242 kg of NPS and 79 kg of urea are the blanket recommendation for the current production of cabbage in Ethiopia. The existing rate is not conducive for farmers. Therefore, small-scale farmers ought to utilize an optimal and cost-effective nitrogen rate to boost the cabbage yield. Furthermore, the effectiveness of nitrogen fertilization is influenced by various factors including the soil type, climate, cabbage variety, and agronomic practices. Integrated nutrient management approaches, combining nitrogen fertilizers with organic amendments or other nutrients, have shown promise in optimizing cabbage production while minimizing environmental impacts. The government ought to heed suggestions concerning soil characteristics such as the soil type, fertility, and additional factors such as the soil pH level and soil moisture contents.

Ethiopia is among the world's poorest nations, and its economy is growing extremely slowly; thus, the government's budget to manage environmental amenities is not always sufficient. Thus, for the provision of environmental management services such as the eradication of Prosopis juliflora, the participation of local households and other stakeholders is crucial. This study is therefore initiated with the objective of assessing rural households' demands for mitigating Prosopis juliflora invasion in the Afar Region of Ethiopia. A multistage sampling technique was employed to obtain the 313 sample rural households that were used in the analysis, and those sample households were selected randomly and independently from the Amibara and Awash Fentale districts of Afar National Regional State, Ethiopia. In doing this, a seemingly unrelated bivariate probit model was used to determine factors affecting rural households' demands for mitigating Prosopis juliflora invasion. Consequently, as per the inferential statistical results, there was a significant mean/percentage difference between willing and nonwilling households for the hypothesized variables, except for some variables such as farm experience; years lived in the area, distance from the market, and dependency ratio. Furthermore, the seemingly unrelated bivariate probit model result indicates that sex, family size, tenure security, livestock holding, frequency of extension contact, and years lived in the area were important factors influencing the willingness to participate in Prosopis juliflora management practices positively, whereas age, off-farm/nonincome, and bid value affected willingness to pay negatively and significantly. Hence, to improve the participation level of households, policymakers should target these variables.