Pub Date : 2018-08-01DOI: 10.1109/CSGB.2018.8544866
S. Michurina, I. Ishchenko, M. A. Cherepanova, S. Arkhipov, Y. Borodin, E. Zavjalov, D. Vasendin
Immunohistochemical analysis and morphometric evaluation of the expression of antiapoptotic Bcl-2 protein and proapoptotic protein Bad in the liver cells of homozygous mice BKS.Cg-Dock7m+/+Leprdb/J (db/db mice) females at different periods of postnatal ontogeny (at the age of 8 and 16 weeks) in the development of obesity and diabetes mellitus type 2 (DM2) were done. In the liver of db/db mice at the age of 8 weeks weak Bcl-2 and Bаd-positive signals were detected in the heterogeneous population of sinusoidal cells of the of liver blood capillaries and in single hepatocytes. It was found that immunohistochemical staining areas of both proteins were increased in mice aged 16 weeks with DM2. However in adult animals, Bcl-2 expression area was almost 2,5 times higher than this parameter value for Bad. This fact indicates the antiapoptotic protection of liver cells and the creation of conditions for blocking the development of apoptosis mitochondrial "branch" at non-alcoholic fatty liver disease (NAFLD) in db/db mice postnatal ontogenesis.
{"title":"Apoptosis in the liver of db/db Mice Female in Postnatal Ontogenesis","authors":"S. Michurina, I. Ishchenko, M. A. Cherepanova, S. Arkhipov, Y. Borodin, E. Zavjalov, D. Vasendin","doi":"10.1109/CSGB.2018.8544866","DOIUrl":"https://doi.org/10.1109/CSGB.2018.8544866","url":null,"abstract":"Immunohistochemical analysis and morphometric evaluation of the expression of antiapoptotic Bcl-2 protein and proapoptotic protein Bad in the liver cells of homozygous mice BKS.Cg-Dock7m+/+Leprdb/J (db/db mice) females at different periods of postnatal ontogeny (at the age of 8 and 16 weeks) in the development of obesity and diabetes mellitus type 2 (DM2) were done. In the liver of db/db mice at the age of 8 weeks weak Bcl-2 and Bаd-positive signals were detected in the heterogeneous population of sinusoidal cells of the of liver blood capillaries and in single hepatocytes. It was found that immunohistochemical staining areas of both proteins were increased in mice aged 16 weeks with DM2. However in adult animals, Bcl-2 expression area was almost 2,5 times higher than this parameter value for Bad. This fact indicates the antiapoptotic protection of liver cells and the creation of conditions for blocking the development of apoptosis mitochondrial \"branch\" at non-alcoholic fatty liver disease (NAFLD) in db/db mice postnatal ontogenesis.","PeriodicalId":230439,"journal":{"name":"2018 11th International Multiconference Bioinformatics of Genome Regulation and Structure\\Systems Biology (BGRS\\SB)","volume":"196 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131626119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.1109/CSGB.2018.8544849
D. Khabarov, M. Kochetkova, I. Siutkina, F. Rakitin, A. Demura, P. Bulychev
Preservation of reproductive function in women with uterine myoma, after surgery for its removal, is of great social significance. One of the pathological processes in the body of a woman who develops in this category of patients before and after surgery is anemia, which arises from pathological hemorrhage from a diseased organ. Therapy of anemia in this category of patients, in practice, is a difficult task, because against a background of recurrent menometrorrhagia, a vicious circle is formed, because of the severity of the patient's condition there are no conditions for conservative myomectomy, and the treatment of anemia, for a short period before the next bleeding - Ineffective. Within the framework of this work, the efficacy of iron carboxymethyltosate and tranexamic acid has been evaluated in organ-saving operations in gynecological practice. It has been established that the therapy of iron deficiency anemia in the preoperative period with iron carbomethyltosate in combination with intraoperative administration of fibrinolysis inhibitors allows avoiding transfusions of homologous donor blood.
{"title":"Technologies of blood saving with the use of tranexamic acid in myomectomy","authors":"D. Khabarov, M. Kochetkova, I. Siutkina, F. Rakitin, A. Demura, P. Bulychev","doi":"10.1109/CSGB.2018.8544849","DOIUrl":"https://doi.org/10.1109/CSGB.2018.8544849","url":null,"abstract":"Preservation of reproductive function in women with uterine myoma, after surgery for its removal, is of great social significance. One of the pathological processes in the body of a woman who develops in this category of patients before and after surgery is anemia, which arises from pathological hemorrhage from a diseased organ. Therapy of anemia in this category of patients, in practice, is a difficult task, because against a background of recurrent menometrorrhagia, a vicious circle is formed, because of the severity of the patient's condition there are no conditions for conservative myomectomy, and the treatment of anemia, for a short period before the next bleeding - Ineffective. Within the framework of this work, the efficacy of iron carboxymethyltosate and tranexamic acid has been evaluated in organ-saving operations in gynecological practice. It has been established that the therapy of iron deficiency anemia in the preoperative period with iron carbomethyltosate in combination with intraoperative administration of fibrinolysis inhibitors allows avoiding transfusions of homologous donor blood.","PeriodicalId":230439,"journal":{"name":"2018 11th International Multiconference Bioinformatics of Genome Regulation and Structure\\Systems Biology (BGRS\\SB)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129047999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.1109/CSGB.2018.8544817
V. Klimontov, D. Bulumbaeva, A. Lykov, O. Fazullina, N. Bgatova, N. Orlov, A. Pfeiffer, O. Pivovarova, N. Rudovich
The aim: to assess the relationships between the circulating Wnt1-inducible signaling pathway protein 1 (WISP1) and the levels of other adipokines, inflammatory markers, fat mass and fat distribution, and parameters of glycemic control in type 2 diabetic subjects. Materials and Methods: We observed 156 patients, 45 M/111 F, including 102 subjects with obesity. The levels of WISP1, hsCRP, alpha1-acid glycoprotein (AGP), and macrophage inflammatory protein 1alpha (MIP-1alpha) were measured in the fasting serum by ELISA. Serum concentrations of leptin, resistin, visfatin, adipsin, adiponectin, IL-6, IL-8, IL-18 and TNF-alpha were determined by Multiplex analysis. Twenty four non-obese non-diabetic subjects, matched by age and sex, were acted as control. The fat mass and distribution was assessed by DEXA. The mean diameter of adipocytes was estimated in the samples of subcutaneous AT in 25 patients. Glucose variability (GV) parameters were derived from continuous glucose monitoring. Results: Patients with diabetes, as compared to control, had significantly higher levels of WISP1 (р=0.02), leptin (p=0.005), resistin (p<0.0001), adipsin (p<0.0001), visfatin (p=0.0003), hsCRP (p<0.0001), AGP (p<0.0001), MIP-1alpha (р=0.006) and IL-6 (p=0.01). Serum WISP1 levels demonstrated positive correlations with percentage of android fat mass (r=0.46, p<0.001), resistin, visfatin and MIP-1alpha levels (r=0.36, r=0.28 and r=0.47 respectively, all p<0.01), but it did not correlated with HbA1c and GV parameters. In a multiple regression analysis android fat mass was the only reliable predictor of serum WISP1 levels. Conclusions: In subjects with type 2 diabetes serum levels of circulating WISP1 are associated with adiposity and adipose tissue dysfunction.
{"title":"Serum levels of WISP1/CCN4 in subjects with type 2 diabetes: the relationships with body fat distribution and adipose tissue dysfunction","authors":"V. Klimontov, D. Bulumbaeva, A. Lykov, O. Fazullina, N. Bgatova, N. Orlov, A. Pfeiffer, O. Pivovarova, N. Rudovich","doi":"10.1109/CSGB.2018.8544817","DOIUrl":"https://doi.org/10.1109/CSGB.2018.8544817","url":null,"abstract":"The aim: to assess the relationships between the circulating Wnt1-inducible signaling pathway protein 1 (WISP1) and the levels of other adipokines, inflammatory markers, fat mass and fat distribution, and parameters of glycemic control in type 2 diabetic subjects. Materials and Methods: We observed 156 patients, 45 M/111 F, including 102 subjects with obesity. The levels of WISP1, hsCRP, alpha1-acid glycoprotein (AGP), and macrophage inflammatory protein 1alpha (MIP-1alpha) were measured in the fasting serum by ELISA. Serum concentrations of leptin, resistin, visfatin, adipsin, adiponectin, IL-6, IL-8, IL-18 and TNF-alpha were determined by Multiplex analysis. Twenty four non-obese non-diabetic subjects, matched by age and sex, were acted as control. The fat mass and distribution was assessed by DEXA. The mean diameter of adipocytes was estimated in the samples of subcutaneous AT in 25 patients. Glucose variability (GV) parameters were derived from continuous glucose monitoring. Results: Patients with diabetes, as compared to control, had significantly higher levels of WISP1 (р=0.02), leptin (p=0.005), resistin (p<0.0001), adipsin (p<0.0001), visfatin (p=0.0003), hsCRP (p<0.0001), AGP (p<0.0001), MIP-1alpha (р=0.006) and IL-6 (p=0.01). Serum WISP1 levels demonstrated positive correlations with percentage of android fat mass (r=0.46, p<0.001), resistin, visfatin and MIP-1alpha levels (r=0.36, r=0.28 and r=0.47 respectively, all p<0.01), but it did not correlated with HbA1c and GV parameters. In a multiple regression analysis android fat mass was the only reliable predictor of serum WISP1 levels. Conclusions: In subjects with type 2 diabetes serum levels of circulating WISP1 are associated with adiposity and adipose tissue dysfunction.","PeriodicalId":230439,"journal":{"name":"2018 11th International Multiconference Bioinformatics of Genome Regulation and Structure\\Systems Biology (BGRS\\SB)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127262526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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