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Effect of certain additives on the quality of boar semen during preservation at 15°C and 5°C 15℃和5℃条件下某些添加剂对猪精液品质的影响
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00006.1
Tejoswita Saikia, K. Ahmed, P. M. Barua, B. Deka, N. Ahmed
The study was aimed to evaluate the effect of four additives viz. KMnO4, Vitamin E, Butylatedhydroxytoluene and trehalose on quality of boar semen during preservation at 15°C and 5°C up to 96 hours. 24 ejaculates were utilized for the study. Sperm motility was significantly higher with BHT irrespective of preservation temperature and period. However, the mean sperm motility was significantly higher (P<0.05) in semen preserved at 15°C than 5°C. Live sperm was significantly (P<0.05) lower in Trehalose than KMNO4, Vitamin E and BHT. The mean percentage of live intact acrosome irrespective of temperature and preservation period was significantly higher (P<0.05) with BHT than others additives. Semen in Modena extender with BHT had significantly higher (P<0.05) percentage of HOST-reacted sperm at different preservation periods irrespective of different temperature. In conclusion, Butylatedhydroxyltoluene (BHT) was found to be superior to KMNO4, Vitamin E and Trehalose for preservation of Hampshire boar semen.
本试验旨在评价4种添加剂KMnO4、维生素E、丁基羟基甲苯和海藻糖在15℃和5℃条件下保存96 h时对公猪精液质量的影响。研究中使用了24种射精。无论保存温度和时间如何,BHT均显著提高了精子活力。然而,15°C保存的精液平均精子活力显著高于5°C保存的精液(P<0.05)。海藻糖对活精子的影响显著(P<0.05)低于KMNO4、维生素E和BHT。添加BHT显著高于其他添加剂(P<0.05),且不受温度和保存期影响。添加BHT的摩德纳鱼在不同保存时间的精液中,无论温度如何,宿主反应精子的比例都显著提高(P<0.05)。综上所述,丁基羟基甲苯(BHT)的保存效果优于KMNO4、维生素E和海藻糖。
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引用次数: 1
Effect of Serum Progesterone Levels at Estrus on Conception in Graded Murrah Buffaloes under Field Conditions 田间条件下发情期血清孕酮水平对分级默拉水牛受孕的影响
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00018.8
M. P. Raj, G. Naidu, M. Srinivas, M. Raghunath, K. A. Rao
The present study was taken up to access the effect of serum progesterone at estrus on conception in Graded Murrah buffaloes under field conditions for which 70 parous buffaloes maintained under village system of rearing, free from apparent pathological abnormalities of the reproductive tract presented in the first postpartum AI were utilized. Serum samples were collected on day 0 and 10 for estimation of progesterone by RIA. The animals were re-examined using ultrasound on day 10 following insemination for determining the CL size and day 30 for pregnancy diagnosis which is confirmed per rectally by day 60 post AI. The mean values of serum progesterone at AI and on day 10 post AI were calculated and the relation was analyzed. Serum progesterone concentration at the time of AI and pregnancy status was negatively correlated indicating that when progesterone level drops < 0.3 ng/ ml (basal level) at the time of AI, the chances of the animal becoming pregnant were more.
本研究以70头未出现第一次产后人工智能中生殖道明显病理异常的分级默拉水牛为研究对象,在野外条件下,探讨了发情期血清孕酮对分级默拉水牛受孕的影响。在第0天和第10天采集血清样本,用RIA法测定孕酮水平。在人工授精后第10天再次进行超声检查以确定CL大小,第30天进行妊娠诊断,并在人工授精后第60天通过直肠检查确认。计算人工授精时和人工授精后第10天血清黄体酮的平均值,并分析两者之间的关系。人工授精时血清黄体酮浓度与妊娠状态呈负相关,表明人工授精时黄体酮水平< 0.3 ng/ ml(基础水平)时,动物受孕的几率更大。
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引用次数: 2
Effect of soya-milk based extender on the physico-morphological parameters of Murrah bull semen during cryopreservation 豆浆膨化剂对默拉公牛精液低温保存过程中物理形态参数的影响
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00013.9
S. Hussain, Sudershan Kumar, M. Iqbal, T. Ahmed
Present investigation was undertaken on three buffalo bulls (Murrah) to study the effect of cryopreservation using Soya-milk extender. A total of 18 collections from three bulls (six collections per bull) were utilized for the study. After initial evaluation, each semen sample was diluted in two different extenders viz. Tris egg yolk extender which acted as control and Soya-milk extender as experimental. Various sperm parameters were studied at equilibration and post-thaw stages. There was no significant difference for percent head abnormalities, percent midpiece abnormalities and acrosome integrity of spermatozoa among extenders at different stages of freezing. The progressive motility and live sperm percentage was significantly higher for Tris egg yolk extender at dilution, equilibration and postthaw stages, but the values for Soya-Milk were also within acceptable limits at all stages of freezing as compared to Tris egg yolk extender. Furthermore the percent tail abnormalities and total sperm abnormalities were significantly higher for soya-milk extender at post thaw. In conclusion the experimental extender could be used as an alternate plant based extender in the cryopreservation of bubaline semen.
本研究以3头水牛为试验对象,研究豆浆膨化剂对其冷冻保存的影响。本研究共使用了来自3头公牛的18个标本(每头公牛6个标本)。初步评价后,每个精液样品用两种不同的扩展剂稀释,即Tris蛋黄扩展剂作为对照,豆浆扩展剂作为实验。在平衡和解冻后阶段研究了精子的各种参数。不同冷冻阶段延长者的精子头部畸形率、中段畸形率和顶体完整性无显著差异。在稀释、平衡和解冻后阶段,Tris卵黄扩展剂的渐进式活力和活精子率显著高于Tris卵黄扩展剂,但与Tris卵黄扩展剂相比,豆浆在所有冷冻阶段的数值也在可接受的范围内。豆浆膨化剂解冻后的尾畸形率和总精子畸形率显著高于其他膨化剂。实验结果表明,该延长剂可作为植物性延长剂用于丁胆碱精液的低温保存。
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引用次数: 1
Bilateral Diffuse Leydig Cell Tumour of Testicles in a Golden Retriever Dog 金毛犬双侧睾丸弥漫性间质细胞瘤
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00014.0
B. Becha, G. Sudha, Sunita Behera, M. Navya
A seven year old male Golden Retriever dog was presented with a history of unilateral scrotal swelling noticed for the last 20 days. The animal was obese and without any clinical signs. Physical examination revealed hard, freely movable enlarged and painless right testicle and hard and nodular left testicle. Ultrasonography revealed circumscribed, hypoechoic and irregular masses within the parenchyma of both testicles suggesting tumour of the testicle. Bilateral orchiectomy was performed as per standard surgical procedure under general anaesthesia. The animal had an uneventful recovery. Histopathology of tumour mass revealed diffuse Leydig cell tumour of both testicles.
一名七岁的雄性金毛寻回犬,在过去的20天里被发现有单侧阴囊肿胀的历史。动物肥胖,无任何临床症状。体格检查显示右侧睾丸坚硬,活动自如,肿大无痛,左侧睾丸坚硬,结节状。超声检查显示双睾丸实质内有边界分明、低回声和不规则肿块,提示睾丸肿瘤。双侧睾丸切除术在全身麻醉下按标准手术程序进行。这只动物平静地康复了。肿瘤组织病理检查显示双睾丸弥漫性间质细胞瘤。
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引用次数: 0
Effect of amla powder supplementation on haematological parameters, ceruloplasmin and transferrin levels in summer stressed murrah buffaloes 添加阿姆拉粉对夏应激穆拉水牛血液学参数、铜蓝蛋白和转铁蛋白水平的影响
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00015.2
P. Lakhani, R. Jindal, S. Nayyar
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引用次数: 1
Atypical Schistosoma Reflexus with Multiple Congenital Abnormalities in Hariana Cow 哈里亚纳牛非典型血吸虫反射伴多重先天性异常
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00017.6
Brijesh Kumar, D. Yadav, Vandana, A. Saxena
The present manuscript reports an unusual case of schistosomus reflexus in a Hariana cow. The clinical findings include free floating viscera, mandibular prognathism, unilateral Amelia, mild hydrocephalus but lacking the typical ventral curvature of spine.
目前的手稿报告了一个不寻常的病例血吸虫反射在哈里亚纳牛。临床表现包括自由浮动脏器,下颌前突,单侧Amelia,轻度脑积水,但缺乏典型的脊柱腹侧弯曲。
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引用次数: 0
Oxytocin, Functions, Uses and Abuses: A Brief Review 催产素,功能,使用和滥用:简要回顾
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00001.2
N. Assad, A. Pandey, L. Sharma
Oxytocin a peptide hormone has the distinction of being the first among the peptide hormones to be sequenced and biochemically synthesized. Oxytocin is secreted from neural as well as non-neural sources inside the body. Among the neural sources supra-optic and paraventricular nuclei of the brain are major sources of secretion, the arcuate nucleus being a minor source. There are many non-neural sources from which synthesis of Oxytocin has been reported. The important ones include interstitial cells of Leydig in testis, epididymis, prostate glands, corpus luteum inside ovary, uterus, oviduct, placenta and adrenal glands. Oxytocin directly acts on the smooth muscle cells and causes muscle contraction. Indirectly it stimulates synthesis of PGF2α inside the female genital tract. As such the Oxytocin is involved in a wide spectrum of activities from milk letdown, parturition, penile erection and ejaculation to social, sexual and maternal behavior. Because of the spectrum of its functions it has since been used therapeutically for conditions like facilitating parturition in uterine inertia, prevent bleeding after parturition and abortion, management of incomplete abortion, milk letdown, retention of placenta etc., although the success has not been predictable. However, Oxytocin is also being misused under the false impression that its use in dairy animals increases the milk yield. Oxytocin abuse is also rampant in fruits and vegetables. This article is an attempt to explore the body of knowledge with respect to functions, uses and most importantly the abuses of this wonderful hormone.
催产素是一种肽激素,在肽激素中是第一个被测序和生化合成的。催产素由体内的神经和非神经源分泌。在神经来源中,视上核和室旁核是主要的分泌源,弓形核是次要的。有许多非神经来源的合成催产素的报道。主要有睾丸间质细胞、附睾间质细胞、前列腺间质细胞、卵巢黄体、子宫间质细胞、输卵管间质细胞、胎盘间质细胞和肾上腺间质细胞。催产素直接作用于平滑肌细胞,引起肌肉收缩。它间接刺激女性生殖道内PGF2α的合成。因此,催产素参与了一系列广泛的活动,从分泌乳汁、分娩、阴茎勃起和射精到社交、性和母性行为。由于其广泛的功能,它已被用于治疗诸如促进子宫惯性分娩,防止分娩和流产后出血,管理不完全流产,乳汁减少,胎盘潴留等情况,尽管成功尚无法预测。然而,催产素也被误用,人们误以为在奶牛身上使用催产素可以增加产奶量。水果和蔬菜中滥用催产素的现象也很普遍。本文试图探索有关这种奇妙激素的功能、用途以及最重要的滥用的知识体系。
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引用次数: 10
Per-vaginal successful management of a rare case of dystocia in murrah buffalo due to dicephalus thoracophagus tetrabrachius tetrapus and dicaudatus monster: A case report 经阴道成功治疗murrah水牛由双头、胸腹、四臂、四尾畸形引起的难产1例
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00004.8
S. Dholpuria, Chander Shekher Saraswat, P. Thanvi, Sunandha Sharma
First report a case of conjoined twin monster (Dicephalus Thoracophagus Tetrabrachius Tetrapus and Dicaudatus) was delivered by per vaginum without fetotomy in a pluriparous Murrah buffalo. The twin monster consist of two male fetus which were possessed separate set of structure two head, two pairs of forelimb and hindlimbs.
本文首次报道了一例在默拉水牛多产时,经阴道分娩的连体双兽(双头、胸食、四臂、四臂、双尾)。双生怪物由两个男性胎儿组成,两个头,两对前肢和后肢具有独立的结构。
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引用次数: 3
A Rare Case of Foetal Maceration in Bitch and its Successful Management 1例罕见母狗胎儿浸渍及其成功处理
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00005.X
A. S. Mahla, S. Shinde, V. Sachan, R. Chaudhari, B. Kumawat, A. Verma, G. Das
A seven years old bitch was presented with the history of whelping signs that appeared five days ago followed by abnormal brownish vaginal discharge since last three days. The body temperature and pulsation was normal without any signs of septicaemia or toxaemia. Per vaginal examination revealed foetal bones in the birth canal but the birth canal was not fully patent. Therapeutic treatment to dilate the birth canal for expulsion of the foetal masses was carried out. After treatment, the foetal masses of three puppies were recovered by applying slight traction with the help of fingers per vaginum. X-ray imaging of pelvic region was done to ensure the complete evacuation of the uterus from foetal bones.
一只七岁的母狗在五天前出现了产崽的迹象,随后三天出现了不正常的褐色阴道分泌物。体温、脉搏正常,无败血症、毒血症征象。阴道检查显示产道内有胎儿骨,但产道未完全通畅。进行了扩张性产道以排出胎儿块的治疗治疗。治疗后,3只幼犬通过手指对阴道的轻微牵引恢复了胎块。骨盆x线成像确保子宫从胎儿骨骼中完全排出。
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引用次数: 2
Retention of Placenta in a Female Camel: A Case Report 雌性骆驼胎盘潴留1例报告
Pub Date : 2016-01-01 DOI: 10.5958/2277-3371.2016.00007.3
Surendra Kumar, G. Purohit, M. Pushp
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引用次数: 1
期刊
Theriogenology Insight - An International Journal of Reproduction in all Animals
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