Theriogenology最新文献

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Effects of osteopontin treatment alone and in combination with hormonal therapy on endometrial epidermal growth factor concentration and fertility at the first insemination after calving in dairy cows 骨桥蛋白单独和联合激素治疗对奶牛产犊后首次授精子宫内膜表皮生长因子浓度和生育力的影响。

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-05 DOI: 10.1016/j.theriogenology.2026.117812

Taiwo Kamardeen Bello , Takashi Tanida , Takayoshi Tagami , Yojiro Yanagawa , Seiji Katagiri

Delayed recovery of cyclic changes in epidermal growth factor (EGF) concentration in the endometrium is related to low conception rates at the first insemination following parturition. An intravaginal infusion of osteopontin (OPN) normalizes EGF profile and improves fertility in repeat-breeder (RB) cows. Therefore, the present study determined whether this treatment can restore normal EGF profile in postpartum cows and improve fertility. First, the effects of recombinant OPN (rOPN) and OPN peptide were examined. In cows with abnormal EGF profile, treatment with both rOPN and OPN peptides increased the normalization rate of EGF profile (78.3 % and 53.1 %, respectively) and conception rates (47.8 % and 61.2 %, respectively), with the rates being higher than those of the control group (25.0 % and 20.0 %, respectively). Second, the effects of combined OPN and hormonal therapy, an established treatment for this abnormality in RB cows, were investigated. Cows were treated with OPN peptide (320 nmol or 1600 nmol) alone and in combination with hormonal therapy. The combined treatment significantly increased EGF normalization and conception rates than the peptide alone (40.5 % vs. 21.9 % and 35.1 % vs. 19.0 %, respectively). The combined treatment achieved the highest conception rate among all groups, including those that received 1600 nmol OPN peptide with or without hormonal therapy. These results indicated that although OPN peptide alone improves fertility, combining it with hormonal therapy could be a better option in cows at the first insemination following parturition.

子宫内膜中表皮生长因子（EGF）浓度周期性变化的延迟恢复与分娩后第一次人工授精的低受孕率有关。阴道内灌注骨桥蛋白（OPN）可使EGF谱正常化并提高重复繁殖奶牛（RB）的生育能力。因此，本研究确定这种处理是否可以恢复产后奶牛正常的EGF水平并提高生育能力。首先，研究了重组OPN （rOPN）和OPN肽的作用。在EGF异常奶牛中，同时使用rOPN和OPN肽可使EGF正常率（分别为78.3%和53.1%）和受孕率（分别为47.8%和61.2%）提高，高于对照组（分别为25.0%和20.0%）。其次，研究了OPN和激素联合治疗（一种针对RB奶牛这种异常的既定治疗方法）的效果。奶牛分别接受320 nmol或1600 nmol的OPN肽单独和激素联合治疗。联合治疗显著提高EGF正常化和受孕率（分别为40.5%比21.9%和35.1%比19.0%）。综合治疗的受孕率在所有组中最高，包括那些接受1600 nmol OPN肽加或不加激素治疗的组。这些结果表明，虽然OPN肽单独使用可以提高奶牛的生育能力，但在奶牛分娩后第一次授精时，将其与激素治疗相结合可能是更好的选择。

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引用次数: 0

Interspecific SCNT in yak followed by embryo aggregation improves early in vitro developmental parameters without restoring pluripotent gene expression 牦牛种间SCNT后的胚胎聚集改善了早期体外发育参数，但不恢复多能基因的表达

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-02 DOI: 10.1016/j.theriogenology.2026.117811

M. Yauri Felipe , M. Duque Rodriguez , A. De Stefano , L.D. Ratner , V. Alberio , D.F. Salamone

The efficiency of interspecific somatic cell nuclear transfer (iSCNT) remains suboptimal, necessitating novel approaches. This study aimed to evaluate the effect of embryo aggregation on the in vitro development of yak clones produced by iSCNT. Zona free cloned embryos were generated using male yak somatic cells and cultured either individually (Y1x, B1x group) or in pairs (Y2x group) in microwells. Aggregation improved the in vitro development of cloned yak embryos, yielding a blastocyst rate of 40.38 % in the Y2x group, which is significantly higher than the 25.34 % and 18.24 % exhibited in Y1x and B1x groups, respectively (P < 0.05), and comparable to the 48.14 % in the IVF control group. Gene expression analysis demonstrated that Y1x blastocysts exhibited significantly lower CDX2 and NANOG levels than those in the Y2x, B1x, and IVF groups. OCT4 levels expression did not differ between groups, and SOX2 levels expression was similar in Y1x, Y2x, and IVF. Y2x embryos showed a higher total cell number than Y1x, though still lower than IVF. In addition, Y2x embryos exhibited fewer CDX2⁺, OCT4⁺, and SOX2⁺ cells compared to B1x and IVF groups. Embryo transfers were performed as a preliminary evaluation of the developmental potential of aggregated embryos. Although early pregnancy signals were detected exclusively in the Y2x group (13 %; 3/25), no embryos were detected at day 42 of gestation. In conclusion, embryo aggregation in yak iSCNT embryos improves selected early in vitro developmental parameters and blastocyst morphology, but does not restore all developmentally relevant gene expression, nor sustain post-implantation development.

种间体细胞核移植（iSCNT）的效率仍然不理想，需要新的方法。本研究旨在探讨胚胎聚集对体外培养牦牛体细胞克隆发育的影响。利用公牦牛体细胞制备无带克隆胚胎，分别在微孔中单独培养（Y1x、B1x组）或成对培养（Y2x组）。聚集促进了克隆牦牛胚胎的体外发育，Y2x组囊胚率为40.38%，显著高于Y1x组的25.34%和B1x组的18.24% (P < 0.05)，与体外受精对照组的48.14%相当。基因表达分析表明，与Y2x、B1x和IVF组相比，Y1x囊胚的CDX2和NANOG水平明显降低。各组间OCT4水平表达无差异，而SOX2水平表达在Y1x、Y2x和IVF中相似。Y2x胚胎的细胞总数高于Y1x，但仍低于IVF。此外，与B1x和IVF组相比，Y2x胚胎显示出较少的CDX2+、OCT4+和SOX2+细胞。胚胎移植是对聚集胚胎发育潜力的初步评估。虽然Y2x组只检测到早期妊娠信号（13%;3/25），但妊娠第42天未检测到胚胎。综上所述，牦牛iSCNT胚胎中的胚胎聚集改善了体外早期发育参数和囊胚形态，但不能恢复所有发育相关基因的表达，也不能维持着床后的发育。

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IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

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IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

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IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

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IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-01

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