Theriogenology最新文献

{"title":"PARL regulates porcine oocyte meiotic maturation by mediating mitochondrial activity","authors":"Naru Zhou ,&nbsp;Zongliang Liu ,&nbsp;Zhenhu Shi ,&nbsp;Lei Luo ,&nbsp;Mengqing Xuan ,&nbsp;Ruiqing Zhu ,&nbsp;Kunlong Hu ,&nbsp;Xinyue Zhu ,&nbsp;Wenhuan Xu ,&nbsp;Yunsheng Li ,&nbsp;Zubing Cao ,&nbsp;Yunhai Zhang","doi":"10.1016/j.theriogenology.2025.01.005","DOIUrl":"10.1016/j.theriogenology.2025.01.005","url":null,"abstract":"<div><div>PARL is a rhomboid membrane protein that plays a crucial role in regulating the metabolism and maintaining the homeostasis of mitochondria which provide important energy and material reserves for oocyte maturation. However, the impact of PARL on oocyte maturation remains poorly understood. Here, we elucidated the pivotal role of PARL in oocyte maturation through its regulatory effects on mitochondrial activity. Specifically, our findings revealed that inhibiting PARL expression by interfering with RNA transcription in oocytes led to a substantial decrease in the rate of first polar body extrusion and early development of parthenogenetically activated embryos. Moreover, PARL deficiency disrupted mitochondrial distribution and activity, leading to the accumulation of ROS, abnormal distribution of CGs and actin, increased tubulin acetylation modification, disturbed spindle assembly and chromosome alignment, ultimately caused DNA damage in porcine oocytes at the metaphase II stage. Intriguingly, PARL deficiency did not cause occurrence of apoptosis in oocytes. Furthermore, our study highlighted that PARL deficiency caused the aberrant expression of genes associated with oocyte maturation, particularly those genes associated with mitochondrial function and DNA integrity. Collectively, these results demonstrate that the indispensable role of PARL in orchestrating porcine oocyte meiotic maturation though its modulation of mitochondrial activity.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 75-85"},"PeriodicalIF":2.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of Se in regulating the proliferation and apoptosis of sheep Leydig cells through the miR-200a/NRF2 pathway","authors":"Kexin Li ,&nbsp;Xiaolei Wang ,&nbsp;Liang Ma ,&nbsp;Youshe Ren ,&nbsp;Lei Shi","doi":"10.1016/j.theriogenology.2025.01.003","DOIUrl":"10.1016/j.theriogenology.2025.01.003","url":null,"abstract":"<div><div>This study aimed to investigate the mechanism by which Se in regulates the proliferation and apoptosis of sheep Leydig cells via the miR-200a/NRF pathway. The cells were isolated and purified from the testes of 8-month-old sheep via a Percoll density gradient. After the cells were treated with different concentrations of Se (0, 2.0, 4.0, 6.0, and 8.0 μmol/L of Se) for 18 h, the miR-200a levels was detected. MiR-200a mimics and inhibitors were transfected into the cells, resulting in five groups (control, NC mimics, miR-200a mimics, NC inhibitor and miR-200a inhibitor). Cell viability and antioxidant status were measured via CCK8 and antioxidant assays, respectively. The abundances of pro-apoptotic (<em>BAX</em>, <em>CASPASE 3</em> and <em>CASPASE 8</em>), cell cycle (<em>P21</em>, <em>P27</em> and <em>CDK1</em>), and NRF2-related (<em>NRF2</em>, <em>HO-1</em>, <em>NQO1</em> and <em>KEAP1</em>) genes were detected by real-time PCR and Western blot analysis.</div><div>The results revealed that miR-200a mimics group presented greater (<em>P</em> &lt; 0.05) abundances of <em>NRF2</em>, <em>HO-1</em> and <em>NQO1</em> mRNA transcripts and proteins. Compared with those both in the NC mimics and the miR-200a inhibitor groups, the activities of GSH-Px and SOD, as well as cell viability in the miR-200a mimics group were significantly greater (<em>P</em> &lt; 0.05). In contrast, the ROS levels, MDA content and abundances of <em>KEAP1</em>, <em>P21</em>, <em>P27</em> and apoptosis-related genes mRNA transcripts and proteins were decreased (<em>P</em> &lt; 0.05). The highest (<em>P</em> &lt; 0.05) miR-200a expression level was detected in the Se_6.0 group. Compared with that in the Se (6.0 μmol/L) group, cell viability in the Se + miR-200a inhibitor group was lower (<em>P</em> &lt; 0.05). The abundances of <em>NRF2</em>, <em>HO-1</em> and <em>NQO1</em> in the Se + miR-200a inhibitor group were lower (<em>P</em> &lt; 0.05) than those in the Se (6.0 μmol/L) group but greater (<em>P</em> &lt; 0.05) than those in the inhibitor group, while <em>KEAP1</em> displayed the opposite trend (<em>P</em> &lt; 0.05).</div><div>These results indicate that Se can activate the NRF2 antioxidant signaling pathway to regulate the proliferation and apoptosis of sheep Leydig cells and that miR-200a plays a vital role in this process. The regulatory effect of Se on male reproduction and spermatogenesis may be related to the number of Leydig cells. This study aimed to provide experimental data for Se regulation of spermatogenesis.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 103-113"},"PeriodicalIF":2.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between activity monitoring device data and ovarian, uterine, hormonal, and pregnancy variables in beef cows","authors":"Cecilia Constantino Rocha ,&nbsp;Ana Beatriz Montevecchio ,&nbsp;Alexandra Bennett ,&nbsp;Abdul Waheed ,&nbsp;Mackenzie Mazziotta ,&nbsp;Tatiane S. Maia ,&nbsp;McKenzie Lane-Jackson Haimon ,&nbsp;Quinn A. Hoorn ,&nbsp;Masroor Sagheer ,&nbsp;Camila J. Cuellar ,&nbsp;Oscar Alejandro Ojeda-Rojas ,&nbsp;Rebecca Lynn Krisher ,&nbsp;Marcello Rubessa ,&nbsp;Ky G. Pohler ,&nbsp;Peter J. Hansen ,&nbsp;Philipe Moriel ,&nbsp;Ricardo C. Chebel ,&nbsp;Mario Binelli","doi":"10.1016/j.theriogenology.2025.01.001","DOIUrl":"10.1016/j.theriogenology.2025.01.001","url":null,"abstract":"<div><div>Implementing accelerometer technologies in beef operations is an alternative to increase precision in estrous detection. We hypothesized that (1) the accelerometer algorithm has similar accuracy in detecting behavioral estrus as does visual observation of pressure-sensitive sensors (estrus patches) in grazing beef cows; (2) variables measured by the accelerometer, such as estrus intensity, are associated with hormonal, ovarian, and uterine variables monitored before, during, and after estrus; and (3) the accelerometer variables are associated with the probability of pregnancy in grazing beef cows submitted to embryo transfer (ET). Fifty cows were fitted with accelerometer and patches to detect estrus after a synchronization protocol in eight subsequent rounds. For each round, only cows that showed estrus (day 0; D0) received ET. Follicular diameter, endometrial thickness, corpus luteum (CL) area, and estradiol (E2) and progesterone (P4) concentrations were measured during proestrus, estrus, and early diestrus. On D7, ET was performed. Pregnancies were diagnosed on D46 and cows recovered for 35D before a new replicate. Patches had a greater accuracy (98 % vs. 91 %) of detection of behavioral estrus than accelerometer algorithm. Cows with lower estrus intensity in the accelerometer had greater follicular diameter on D0 (P = 0.022), CL area on D4 and D7 (P = 0.05), endometrial thickness on D-1 (P = 0.10), and reduced E2 concentrations on D-1 (P = 0.0032). The accelerometer variables did not predict accurately the probability of pregnancy/ET. In conclusion, visual observation of patches was more accurate in detecting estrus than the accelerometer algorithm and most of the associations between accelerometers and physiological variables were for characteristics measured at proestrus.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 64-74"},"PeriodicalIF":2.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro study of carbetocin, an oxytocin receptor agonist, and 4-phenylfuroxan-3-carbonitrile, a NO-releasing agent, as cervical dilatators in sheep","authors":"García-Barcelo Pedro ,&nbsp;Romero Angel ,&nbsp;Rodríguez-Piñón Marcelo ,&nbsp;Cerecetto Hugo","doi":"10.1016/j.theriogenology.2025.01.002","DOIUrl":"10.1016/j.theriogenology.2025.01.002","url":null,"abstract":"<div><div>The aim was to study the effect of 4-phenylfuroxan-3-carbonitrile (Fx), a NO-releasing agent, and carbetocin, an oxytocin receptor agonist, on matrix metalloproteinases-2 (MMP-2) activity and PGE2 production in cervix from cycling sheep. Cervical explants were incubated during 12 h with MEM supplemented with increasing concentrations of Fx in DMSO (2 %) (0 to 300 μg/mL) with Cb (100 ng/mL) (Experiment 1, n = 15) and DMSO (2 %), DMSO + Cb (100 ng/mL) or DMSO + Fx (30 μg/mL) (Experiment 2, n = 10), and their respective controls. In the supernatants, activated (A) and latent (L) MMP-2 activities were determined by a SDS-PAGE zymography, PGE2 concentration by immunoassay and NO production indirectly as nitrites by spectrophotometry. Data were analyzed by ANOVA. The Cb treatment increase the A MMP-2 activity in DMSO (Experiment 1 at follicular phase and Experiment 2) or alone (Experiment 2) and increase the L MMP-2 activity (Experiments 1 and 2) (P &lt; 0.02). The DMSO treatment also increase the L MMP2 activity (Experiment 2) (P &lt; 0.0001). Treatment with Fx + DMSO increased the concentration of accumulated nitrites in the supernatant (P &lt; 0.0001) (Experiment 1), but did not affect or decrease the activity of A and L MMP-2 (P &lt; 0.04) (Experiments 1 and 2). The PGE2 concentration trend to increase with Cb treatment (P = 0.0614) and decrease with Fx+DMSO treatment (P &lt; 0.0001) (Experiment 2). In conclusion, Cb and/or DMSO treatment of cervical explants increase the MMP-2 activity through PGE2-independent mechanisms, but Fx in DMSO fail in this, suggesting that the pre-treatment with Cb and/or DMSO could be used to increase cervical dilation in ewes.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 168-174"},"PeriodicalIF":2.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ReBreed21-ET: Evaluation of a rapid resynchronization program that allows timed embryo transfer every 21 days","authors":"João Paulo N. Andrade ,&nbsp;Victor E. Gomez-León ,&nbsp;Guilherme Madureira ,&nbsp;Luma C. Sartori ,&nbsp;Gustavo F. Grillo ,&nbsp;Rafael R. Domingues ,&nbsp;Meliton Fosado ,&nbsp;Rodrigo V. Sala ,&nbsp;Milo C. Wiltbank","doi":"10.1016/j.theriogenology.2024.12.031","DOIUrl":"10.1016/j.theriogenology.2024.12.031","url":null,"abstract":"<div><div>This study evaluated the efficiency of a rapid resynchronization of ovulation program to allow timed embryo transfer (TET) every 21d in heifer embryo recipients. Holstein heifers (n = 510) had synchronized ovulation using a modified 5d CoSynch program for a TET (D7) after induced ovulation (D0). After TET, heifers were blocked by number of previous TETs and randomized into one of two resynchronization of ovulation programs: Resynch28 (n = 279), a traditional resynch program for TET 35d after previous TET; or ReBreed21-ET, a program designed to allow TET 21d after previous TET. Once assigned into one of the two programs, heifers were kept in the same program during a 105-d TET period. In Resynch28, heifers received an intravaginal progesterone (P4) insert on D28, on D33 the P4 was removed, and pregnancy diagnosis was performed using rectal ultrasound to determine embryonic heartbeat. Nonpregnant heifers received a PGF2α treatment with a later GnRH treatment on D35 for a potential TET on D42 (35d after previous TET). Heifers in ReBreed21-TET received an intravaginal P4 insert on D14, on D19 the P4 was removed, and on D21 a GnRH treatment was given to synchronize a new ovulation. On D28, pregnancy diagnosis was performed using transrectal ultrasound to detect an embryonic heartbeat and nonpregnant heifers that had a corpus luteum (CL) ≥18 mm in diameter received a TET (21d after the previous TET). Pregnancy per ET (P/ET) from the first TET was greater for heifers in ReBreed21-ET (52 %) than Resynch28 (39.4 %). In contrast, the subsequent TET (second and later) had similar P/ET for ReBreed21-ET (40.4 %) and Resynch28 (40.8 %). The overall pregnancy loss from D28 to D63 did not differ between programs (ReBreed21-ET [18.5 %] and Resynch28 (16.3 %]). Nevertheless, there were fewer pregnancy losses from D28 to D33 for Resynch28 (3.5 %) than ReBreed21-ET (10.1 %), while from D33 to D47, there was greater pregnancy loss for Resynch28 (10.1 %) than ReBreed21-ET (4.9 %). Time to pregnancy was approximately 8d earlier for ReBreed21-ET (45 ± 3d) than Resynch28 (53 ± 3d). Overall cumulative pregnancies at the end of a 105-d TET season were greater for ReBreed21-ET (75.2 %) than Resynch28 (64 %). Thus, the ReBreed21-ET program can improve the efficiency of TET programs.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 145-151"},"PeriodicalIF":2.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GM-CSF treatment of frozen bovine sperm improves function, fertilization, and subsequent embryo development","authors":"Annie L. Whitty ,&nbsp;Karen L. Kind ,&nbsp;Kylie R. Dunning ,&nbsp;Nicole O. McPherson ,&nbsp;Mark B. Nottle","doi":"10.1016/j.theriogenology.2024.12.022","DOIUrl":"10.1016/j.theriogenology.2024.12.022","url":null,"abstract":"<div><div>In vitro embryo production (IVP) is used in the cattle industry to increase the rate of genetic gain. IVP uses semen that has been frozen and thawed, a process that renders sperm less viable than sperm from fresh semen. Granulocyte macrophage colony stimulating factor (GM-CSF) is present in bovine seminal plasma, while its receptor is present on bovine sperm. The present study aimed to determine if GM-CSF could improve the function and quality of frozen-thawed bovine sperm and embryo development following in vitro fertilization. Thawed bovine sperm (n = 3 bulls with 3 replicates per bull) was incubated with 0, 0.1, 1, 2 or 10 ng/ml of recombinant bovine GM-CSF in buffered wash media for 45 min and assessed for motility, glucose uptake, mitochondrial activity, intracellular calcium, capacitation, DNA integrity, and in vitro embryo development. The addition of 1, 2, and 10 ng/ml GM-CSF increased total motility (P = 0.02, P = 0.007, P = 0.01), progressive (P = 0.02, P = 0.03, P = 0.01), and rapid motility (P = 0.01, P = 0.01, P = 0.01), while 10 ng/ml increased glucose uptake (P = 0.003), and 1, 2, and 10 ng/ml increased capacitation (P = 0.003, P = 0.001, P = 0.0003). There was no difference between groups for mitochondrial activity, intracellular calcium, or DNA integrity. GM-CSF treatment of sperm prior to in vitro insemination increased fertilization rate (P = 0.01), hatching blastocyst rate (P = 0.05), and blastocyst inner cell mass cell number (P = 0.03) compared with control. In conclusion, GM-CSF treatment of frozen-thawed bovine sperm improves sperm function and quality resulting in increased fertilization capacity and subsequent embryo development, suggesting it may improve cattle IVP efficiencies.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 46-55"},"PeriodicalIF":2.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of PI3K/AKT signaling pathway during capacitation","authors":"Woo-Jin Lee ,&nbsp;Jae-Hwan Jo ,&nbsp;Claudine Uwamahoro ,&nbsp;Seung-Ik Jang ,&nbsp;Eun-Ju Jung ,&nbsp;Jeong-Won Bae ,&nbsp;Joonho Moon ,&nbsp;Dae-Hyun Kim ,&nbsp;Jun Koo Yi ,&nbsp;Jae Jung Ha ,&nbsp;Dong Yep Oh ,&nbsp;Woo-Sung Kwon","doi":"10.1016/j.theriogenology.2024.12.026","DOIUrl":"10.1016/j.theriogenology.2024.12.026","url":null,"abstract":"<div><div>Spermatozoa must undergo a complex maturation process within the female genital tract known as capacitation. This process entails the phosphorylation or dephosphorylation of various proteins, and multiple signaling pathways are recognized to play a role. The present study aims to identify alterations in the expression of proteins related to the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT) signaling pathway and assess sperm functions during capacitation. Mouse spermatozoa were incubated in a medium supplemented with bovine serum albumin to induce capacitation. Subsequently, we evaluated sperm motility, cell viability, capacitation status, and acrosome reaction. Consequently, we observed a significant increase in several kinematic parameters. Additionally, the capacitation status and acrosome reaction exhibited a time-dependent manner. Furthermore, we confirmed a significant increase in the phosphorylation of PI3K, PDK1, and p-AKT (Thr308), along with activation of PKA and tyrosine phosphorylation. These alterations in protein expression were found to correlate with capacitation status, acrosome reaction, and various kinematic parameters. Therefore, our findings show that the phosphorylation of PKA and PI3K/AKT pathway-related proteins during capacitation may plays a crucial role in regulating sperm function. These findings contribute to a better understanding of the molecular mechanisms and interactions of the PI3K/AKT signaling pathway in the capacitation process.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 94-102"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing oocyte recovery during ultrasound-guided follicle aspiration in mares: A postmortem study","authors":"Adrián Márquez-Moya,&nbsp;Laura Sala-Ayala,&nbsp;Nerea Carreras-Vico,&nbsp;Rebeca Martínez-Boví,&nbsp;Juan Cuervo-Arango","doi":"10.1016/j.theriogenology.2024.12.032","DOIUrl":"10.1016/j.theriogenology.2024.12.032","url":null,"abstract":"<div><div>The objectives of this study were to investigate the influence of different OPU variables (vacuum and injection pressures during follicle aspiration and flushing, needle twisting to scrape follicles, number of follicle flushes and type of flushing media) on oocyte recovery rate (ORR) and morphology. Overall, 120 <em>postmortem</em> excised ovaries were processed in 62 replicates (1.9 ± 0.7 ovaries per replicate), with a total of 1336 follicles punctured and aspirated (11.1 ± 6.2 follicles per ovary) by ultrasound-guided follicle aspiration resembling the OPU procedure from live mares. The aspiration pressure (50 vs. 300 mmHg) did not influence (P &gt; 0.1) ORR (57.1 and 55.1 %, respectively) but the higher pressure (1.9 mL/s aspiration flow) resulted in the recovery of more denuded oocytes than the lower pressure (0.8 mL/s) (66 % vs 42 %, P &lt; 0.05). High injection pressure to flush follicles (1.5 mL/s) was associated with lower (P &lt; 0.05) ORR (31 %) and higher loss of oocytes in the leaked fluid (10 % of aspirated follicles) than lower pressures (37 % and 47 % ORR for 0.3 and 0.8 mL/s injection flow rates, respectively). The number of flushes per follicle influenced (P &lt; 0.05) the ORR: 28.8, 46.1, 42.7, 43.0 and 64.2 % for x0, x1, x3, ×6 and ×10 flushes groups. More oocytes (P &lt; 0.05) were lost in the leaked fluid outside the ovary in groups ≥ ×3 (5–7%) than in ×0 and ×1 flushes (&lt;1 %). The ORR was superior following scraping of follicle wall by twisting the needle (55.2 %) than no scraping (42.9 % ORR), while the type of flushing media (0.9%NaCl + heparin vs commercial flushing media with PVA) did not influence ORR. In conclusion, follicle scraping by needle twisting and follicle flushing during 10 times yielded the highest oocyte recovery. Repeated follicle flushing with high injection pressure was associated with a higher loss of oocytes outside the ovary in the leaked fluid.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 39-45"},"PeriodicalIF":2.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analyses of transcriptomes, metabolomes, and proteomes unveil the role of FoXO signaling axis in buck semen cryopreservation","authors":"Tao Zhong ,&nbsp;Shun Wu ,&nbsp;Guolin Chen,&nbsp;Siyuan Zhan,&nbsp;Linjie Wang,&nbsp;Jiaxue Cao,&nbsp;Jiazhong Guo,&nbsp;Li Li,&nbsp;Hongping Zhang,&nbsp;Lili Niu","doi":"10.1016/j.theriogenology.2024.12.027","DOIUrl":"10.1016/j.theriogenology.2024.12.027","url":null,"abstract":"<div><div>Sperm cryopreservation is a complex process involving gene expression, protein synthesis, membrane stability, and metabolic adaptation. However, molecular alterations in sperm cryopreservation and the mechanisms defending against freezing damage remain poorly understood. This study investigates these changes and defense mechanisms using transcriptomics, proteomics, and metabolomics data. During sperm cryopreservation, the expression level of G protein subunit alpha i3 (<em>GNAI3</em>) was significantly downregulated in post-thaw sperm (<em>P</em> &lt; 0.001), while matrix metallopeptidase 9 <em>(MMP9</em>) was upregulated compared to FS groups (<em>P</em> &lt; 0.01). Additionally, interleukin 6 (<em>IL6</em>) expression in the CS group showed an approximate increase (<em>P</em> &lt; 0.05), whereas ribosomal protein S27a (<em>RPS27A</em>) expression decreased markedly (<em>P</em> &lt; 0.05). Other important molecules such as macrophage stimulating 1 receptor (<em>MST1R</em>), hypoxia-inducible factor 1 subunit alpha (<em>HIF1A</em>), fibroblast growth factor 8 (<em>FGF8</em>), CD9 molecule (CD9), peptidase D (PEPD) and terminal nucleotidyltransferase 5B (TENT5B) also exhibited significant changes in expression (<em>P</em> &lt; 0.05). Moreover, the study revealed the regulatory roles of metabolites such as glucose and glutamic acid during sperm cryopreservation. The involvement of catalase (CAT) protein in antioxidant defense was also noted. The interactions among mRNAs, miRNAs, proteins, and metabolites highlight the critical role of the FoxO signaling pathway in modulating responses to freezing. Our study reveals the molecular regulatory mechanisms of sperm during cryopreservation, emphasizing the importance of the FoxO pathway and specific metabolites in response to cryo-injury. These findings provide deeper insights into the complexity of sperm cryobiology and offer practical guidance for optimizing sperm cryopreservation.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 19-30"},"PeriodicalIF":2.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marked blastomere herniation is the only post-thaw morphological characteristic associated with a reduced pregnancy rate for in vitro produced equine blastocysts","authors":"L.C. Arnold ,&nbsp;T.A.E. Stout ,&nbsp;M.M. Beitsma ,&nbsp;C.C. Deelen ,&nbsp;M. Rizzo ,&nbsp;B. Leemans ,&nbsp;C. Galli ,&nbsp;S. Colleoni ,&nbsp;G. Lazzari ,&nbsp;M. Barandalla ,&nbsp;A.N.J. Claes","doi":"10.1016/j.theriogenology.2024.12.028","DOIUrl":"10.1016/j.theriogenology.2024.12.028","url":null,"abstract":"<div><div>It is not known whether the likelihood of pregnancy after transfer of cryopreserved equine <em>in vitro</em> produced (IVP) blastocysts is related to post-thaw morphological characteristics, or could be impacted by other factors such as embryo biopsy prior to cryopreservation. This study examined associations between post-thaw morphological appearance of equine IVP embryos, and biopsy prior to slow-freezing, on the likelihood of pregnancy. Biopsy of IVP blastocysts was performed by harvesting cells herniated through the intra-cytoplasmic sperm injection (ICSI)-derived hole in the zona pellucida (ZP). Morphological characteristics (shape, extent of presumptive trophectoderm layer, % extruded blastomeres, expansion and % herniated cells) of frozen-thawed, biopsied (n = 36) and non-biopsied (n = 444) IVP blastocysts were recorded prior to transfer, and pregnancy outcome was recorded. A binomial multivariate generalized linear model was used to identify factors influencing pregnancy outcome. The overall pregnancy rate was 73.3 %, but was significantly affected by the interval between ICSI and confirmation of blastocyst formation, with lower pregnancy rates for later developing embryos (p &lt; 0.01). Furthermore likelihood of pregnancy was significantly influenced by the stallion used for ICSI (p &lt; 0.01) and the biological mother (p = 0.02), although no single biological sire or dam could be identified as contributing significantly. The biopsy procedure <em>per se</em> did not compromise pregnancy outcome (p = 0.59). On the other hand, pregnancy was less likely (p = 0.02) for blastocysts with more than 10 % herniated cells post-thaw (39 %; n = 13) than for those with fewer than 10 % (69 %; n = 166) or no (77 %; n = 301) herniated cells. No other post-thaw morphological characteristic affected pregnancy outcome. In conclusion, the sporadic herniation of a large part of the embryo through the ICSI-derived hole in the ZP during thawing negatively influences pregnancy outcome.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 1-8"},"PeriodicalIF":2.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}