Theriogenology最新文献

{"title":"Periparturient oleic acid-rich fat supplementation affects the lipid profile in blood and results in an increased oocyte yield in postpartum dairy cows","authors":"F. Piscopo ,&nbsp;B. Gasparrini ,&nbsp;R. van Halderen ,&nbsp;J.F. Brouwers ,&nbsp;J. van den Broek ,&nbsp;H.T.A. van Tol ,&nbsp;P.L.A.M. Vos ,&nbsp;H. Aardema","doi":"10.1016/j.theriogenology.2025.01.018","DOIUrl":"10.1016/j.theriogenology.2025.01.018","url":null,"abstract":"<div><div>In high-producing dairy cows periparturient negative energy balance (NEB) triggers body fat mobilization, resulting in elevated blood levels of non-esterified fatty acids (NEFAs). Blood is dominated by the saturated fatty acids (SFA) palmitic (C16:0) and stearic acid (C18:0), which are associated with a negative effect on oocyte developmental competence. In contrast, the monounsaturated fatty acid (MUFA) oleic acid is harmless and is able to counteract the negative effect of saturated NEFAs on in vitro maturing oocytes. Since preantral follicles lack oleic acid-rich follicular fluid, we hypothesized that preantral follicles and oocytes may benefit from oleic acid-rich fat supplementation during NEB.</div><div>Eight-month pregnant Holstein Friesian heifers were randomly divided in two groups to receive a standard, palmitic acid-rich (CTR, n = 5), or rumen-protected oleic acid-rich (UNSAT, n = 6), periparturient fat supplementation until 4 weeks post-calving. NEFA, β-Hydroxybutyric acid and haptoglobin profiles in blood were monitored, and cumulus-oocyte-complexes (COCs) were via transvaginal ovum pick-up (OPU) collected at 8, 12, and 16 weeks postpartum for in vitro maturation, fertilization (day 0), and culture until day 8.</div><div>Oleic acid supplementation increased C18:1 and reduced C16:0 levels in blood in comparison to CTR, during the peripartum period. Interestingly, the UNSAT group exhibited a 1.6-times higher oocyte yield in comparison to the CTR, but no difference in oocyte developmental competence between the groups.</div><div>These findings suggest that peripartum oleic acid supplementation supports follicles and oocytes during NEB. Potential long-term benefits of oleic acid on fertility in dairy cows, in a higher number of animals, warrant further investigation.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"236 ","pages":"Pages 33-44"},"PeriodicalIF":2.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transglutaminase 2 reduces the polyspermy of porcine oocytes by promoting the hardening of zona pellucida","authors":"Zhaokang Cui ,&nbsp;Jianli Zhang ,&nbsp;Jun Zhang ,&nbsp;Jifeng Zhong ,&nbsp;Huili Wang ,&nbsp;Bo Xiong","doi":"10.1016/j.theriogenology.2025.01.023","DOIUrl":"10.1016/j.theriogenology.2025.01.023","url":null,"abstract":"<div><div>Polyspermy is a type of abnormal fertilization that would increase the risk of embryonic death and hence reduced female fecundity. To avoid this occurrence, mammalian oocytes develop the complicated mechanisms of blocks to polyspermy. Our previous study discovered that transglutaminase 2 (Tgm2) is a newly identified molecule responsible for the post-fertilization block to sperm penetration through zona pellucida (ZP) and polyspermy in mice. However, whether this function of Tgm2 is conserved across species has not been clarified. This study applied the porcine oocytes as a model to address this question. It was observed that inhibition of TGM2 activity by using its specific inhibitor LDN-27219 exhibited a substantially increased rate of polyploid zygotes after <em>in vitro</em> fertilization. In addition, our study validated that recombinant TGM2 protein interacted with zona pellucida glycoprotein 3 (ZP3) and crosslinked it to promote the ZP hardening. Further, incubation of porcine oocytes with recombinant TGM2 protein effectively reduced the occurrence of polyploid zygotes. Collectively, our data reveal that TGM2 performs a conserved function in porcine oocytes for zona block and polyspermy prevention.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"236 ","pages":"Pages 8-12"},"PeriodicalIF":2.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive characteristics of Japanese quail hatched from eggs incubated at different temperatures","authors":"Alexandre Vinhas de Souza ,&nbsp;Ana Patrícia Alves Leão ,&nbsp;Carina Fernanda Gomes da Silva ,&nbsp;Juliano Vogas Peixoto ,&nbsp;Bruna Resende Chaves ,&nbsp;Sarah Conceição Andrade ,&nbsp;Édison José Fassani ,&nbsp;Renata Ribeiro Alvarenga ,&nbsp;Márcio Gilberto Zangeronimo","doi":"10.1016/j.theriogenology.2025.01.022","DOIUrl":"10.1016/j.theriogenology.2025.01.022","url":null,"abstract":"<div><div>The objective of this study was to evaluate the influence of different incubation temperatures (ITs) on the posthatch reproductive characteristics of Japanese quails (<em>Coturnix japonica</em>). A total of 1332 fertile eggs were incubated at different temperatures: 36.0, 37.5 and 39.0 °C. After hatching, the birds were transferred to rearing cages until 35 days of age and, later, to production cages in groups of nine females and three males. The experimental design was a randomized block with three treatments and six replications. IT did not influence (P &gt; 0.05) birth weight or hatch window; however, higher hatchability was observed (P &lt; 0.05) in eggs incubated at 37.5 °C. There was no effect (P &gt; 0.05) of IT on the relative weight of organs at 35 and 60 days of age or on the male growth curve. In females, body weight at growth maturity was higher (P &lt; 0.01) when the IT was 39.0 °C. The characteristics of the semen were not influenced (P &gt; 0.05) by IT, except for sperm viability, which was higher (P &lt; 0.05) when temperatures of 37.5 and 39.0 °C were used. There was no effect (P &lt; 0.05) of IT on egg fertility or on the morphological characteristics of female reproductive organs. However, a lower age at first egg were observed (P &lt; 0.05) when the IT was 39.0 °C. There was no effect (P &gt; 0.05) of IT on egg quality, except for yolk height, which was higher (P &lt; 0.01) at 36.0 and 39.0 °C. It is concluded that a temperature of 37.5 °C should be used during the incubation of Japanese quail eggs.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"236 ","pages":"Pages 1-7"},"PeriodicalIF":2.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paradoxical effects of inhibition of Δ14-reductase and Δ7-reductase on porcine oocyte maturation and subsequent embryo development after parthenogenetic activation","authors":"Sanghoon Lee ,&nbsp;Dabin Cha ,&nbsp;Jun-Xue Jin ,&nbsp;Geon A. Kim ,&nbsp;Byeong Chun Lee","doi":"10.1016/j.theriogenology.2025.01.021","DOIUrl":"10.1016/j.theriogenology.2025.01.021","url":null,"abstract":"<div><div>Follicular fluid-derived meiosis-activating sterol (FF-MAS), an intermediate in the cholesterol biosynthesis pathway, plays a crucial role in the meiotic resumption of mammalian oocytes. Maintaining a high concentration of FF-MAS <em>in vitro</em> is challenging; therefore, AY9944 A-7, an inhibitor of Δ14-reductase [which converts FF-MAS to testis meiosis-activating sterol (T-MAS)] and Δ7-reductase (which converts T-MAS to cholesterol), has been used to enhance oocyte maturation. This study examined the effects of various concentrations (0, 10, 20, and 40 μM) of AY9944 A-7 on porcine oocyte maturation and subsequent embryo development. Results indicate that treatment with 10 and 20 μM AY9944 A-7 during <em>in vitro</em> maturation (IVM) enhanced oocyte nuclear maturation, with 10 μM significantly increasing the transcript expression of oocyte maturation-related genes. However, blastocyst formation rates significantly decreased in oocytes treated with AY9944 A-7 concentrations above 10 μM. To explore these unexpected findings, the study evaluated the effects of AY9944 A-7 on lipid content in oocytes and the sonic hedgehog (SHH) signaling pathway in subsequent parthenogenetic embryos. A concentration-dependent decrease in oocyte lipid content was observed following AY9944 A-7 treatment. Additionally, transcripts of SHH signaling pathway genes were detected in preimplantation-stage parthenogenetic embryos, with reduced expression in the 10 μM AY9944 A-7-treated group. Taken together, AY9944 A-7 supplementation during porcine IVM enhanced oocyte maturation by accumulating FF-MAS, but subsequent embryo development was impaired due to cholesterol deficiency, potentially mediated by SHH signaling downregulation.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 245-253"},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of KIFC1 activity induces spindle instability and actin defects during porcine oocyte maturation","authors":"Yu-Ran Wang ,&nbsp;Peng-Jie Wang ,&nbsp;Le-Yan Tao ,&nbsp;Lin-Lin Hu ,&nbsp;Qiang-Qiang Liu ,&nbsp;Shao-Chen Sun ,&nbsp;Jing-Xi Wei ,&nbsp;Yue Wang","doi":"10.1016/j.theriogenology.2025.01.012","DOIUrl":"10.1016/j.theriogenology.2025.01.012","url":null,"abstract":"<div><div>KIFC1 is a motor protein of the Kinesin family and it is involved in spindle apparatus assembly, chromosome arrangement, and microfilament-mediated biological processes in mitosis. However, the specific function of KIFC1 in pig oocytes remains unclear. Here, in order to explore the function of KIFC1 in porcine oocytes, the AZ82 inhibitor was used to inhibit the activity of KIFC1. Our results showed when KIFC1 was inhibited, the polar body extrusion rate was obviously decreased, indicating that KIFC1 plays a crucial role in porcine oocytes. We next measured the spindle structure and chromosome arrangement via immunofluorescent staining and found both the rates of abnormal spindle and chromosome disorder increased significantly. By further analyzing the causes of the abnormal spindle, we found the acetylation of tubulin was disrupted. In addition, we also found the spindle position was impaired after KIFC1 inhibition, declaring the spindle migration was affected. Further analysis found cortex actin decreased and cytoplasmic actin increased after KIFC1 inhibition. In summary, we found that KIFC1 played a critical role in porcine oocytes maturation by controlling spindle apparatus via mediating the acetylation of microtubule and regulating the spindle migration via affecting actin dynamics.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 254-261"},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Optimization of lipofection protocols for CRISPR/Cas9 delivery in porcine zona pellucida intact oocytes: A study of coincubation duration and reagent efficacy” [Theriogenology 230 (2024) 121–129]","authors":"C. Piñeiro-Silva ,&nbsp;J. Gadea","doi":"10.1016/j.theriogenology.2025.01.014","DOIUrl":"10.1016/j.theriogenology.2025.01.014","url":null,"abstract":"","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 194-195"},"PeriodicalIF":2.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ascorbic acid and resveratrol improve the structural integrity of the extracellular matrix and enhance follicular survival in cultured bovine ovarian tissue","authors":"F.C. Costa ,&nbsp;B.R. Silva ,&nbsp;F.F.C. Filho ,&nbsp;V.S. Bezerra ,&nbsp;V.A.N. Azevedo ,&nbsp;A.A. Silva ,&nbsp;J.R.V. Silva","doi":"10.1016/j.theriogenology.2025.01.020","DOIUrl":"10.1016/j.theriogenology.2025.01.020","url":null,"abstract":"<div><div>This study aimed to investigate the changes induced by the culture system and the effect of ascorbic acid and resveratrol on collagen fibers, stromal cells, follicle growth and survival, as well as antioxidant enzyme activity in cultured bovine ovarian tissues. In experiment 1, bovine ovarian fragments were cultured in α-minimum essential medium (α-MEM⁺) for 6 days. Before and after culturing, the fragments were fixed and processed to assess follicular morphology and diameters, stromal cell survival, collagen fibers, and glycosaminoglycans (GAGs). Uncultured and cultured tissues were also used to measure mRNA expression for superoxide dismutase (<em>SOD</em>), catalase (<em>CAT</em>), glutathione peroxidase (<em>GPX</em>), and peroxiredoxin (<em>PRDX</em>). Thiol levels and activity of CAT, SOD, and GPX enzymes were also investigated. In experiment 2, bovine ovarian fragments were cultured in α-MEM⁺ alone or supplemented with 50 μg/mL ascorbic acid or both 50 μg/mL ascorbic acid and 20 μM resveratrol for 6 days. In experiment 1, cultured tissues had higher percentages of growing follicles, but higher percentage of degenerated follicles than uncultured slices (P &lt; 0.05). Additionally, the collagen and GAGs network became disorganized, with reduced deposition around primordial and primary follicles (P &lt; 0.05). The number of stromal and granulosa cells, as well as follicular and oocyte diameters were reduced in both follicular categories compared to uncultured tissue (P &lt; 0.05). Expression of mRNA for <em>CAT, SOD, GPX</em>, and <em>PRDX</em> was downregulated in 6-day cultured tissues (P &lt; 0.05). Similarly, thiol levels and CAT activity were also reduced (P &lt; 0.05). In experiment 2, ascorbic acid or both ascorbic acid and resveratrol increased the rate of follicular diameters and survival, and the number of granulosa and stromal cells compared to tissues cultured in the control medium (P &lt; 0.05). Both ascorbic acid and resveratrol improved collagen density and preserved the GAG network, as well as increased thiol levels and CAT activity (P &lt; 0.05). In conclusion, <em>in vitro</em> culture of ovarian tissue favored follicular activation, but reduced the proportion of normal follicles, collagen, GAG network, stromal cell numbers, and tissue antioxidant protection. Ascorbic acid alone or in association with resveratrol improved the preservation of extracellular matrix components and enhanced follicular survival.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 231-244"},"PeriodicalIF":2.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulus cells and the TNF-alpha signaling facilitate aging of ovine oocytes","authors":"Shuai Gong ,&nbsp;Yan Zhang ,&nbsp;Chao-Qun Cong,&nbsp;Guo-Liang Wang,&nbsp;Lin Jiang,&nbsp;Hong-Jie Yuan,&nbsp;Jing-He Tan,&nbsp;Ming-Jiu Luo","doi":"10.1016/j.theriogenology.2025.01.019","DOIUrl":"10.1016/j.theriogenology.2025.01.019","url":null,"abstract":"<div><div>Post-maturation oocyte aging (PMOA) is known to significantly impair the developmental potential of oocytes; however, comprehensive studies on ovine PMOA remain limited. In mice, <em>cumulus</em> cells (CCs) accelerate oocyte aging by releasing cytokines, but the roles of CCs and cytokines in PMOA of domestic animals are poorly understood. This study aimed to elucidate the involvement of CCs and tumor necrosis factor (TNF)-α in the PMOA of ovine oocytes. Our findings reveal that PMOA significantly reduced blastocyst rates and the expression of development-promoting genes, while increasing oocyte degeneration and activation rates, along with expression of development-inhibiting genes, compared to newly matured oocytes. These detrimental effects were more pronounced in oocytes aged as <em>cumulus</em>-oocyte complexes than as <em>cumulus</em>-denuded oocytes. Additionally, PMOA led to increased apoptotic rates, TNF-α production, and TNF receptor 1 (TNFR1) expression in CCs, coupled with a significant reduction in the expression of anti-apoptotic genes. Mature oocytes expressed TNFR1, with levels decreasing significantly during PMOA. Importantly, the addition of the TNF-α antagonist Etanercept to the aging medium markedly improved parthenogenetic embryo development and the expression of competence-related genes, while mitigating CC apoptosis during PMOA of COCs. In conclusion, PMOA compromises developmental potential while heightening oocyte degeneration and activation sensitivity in ovine oocytes. Cumulus cells exacerbate PMOA through increased TNF-α signaling activity, highlighting the potential of TNF-α antagonists as therapeutic agents to counteract the deleterious effects of PMOA.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 210-220"},"PeriodicalIF":2.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudopregnancy in dromedary camels: Characterization, prevalence, and clinical and hormonal properties","authors":"Khalid S. Almushawwah ,&nbsp;Derar R. Derar ,&nbsp;Ahmed Ali","doi":"10.1016/j.theriogenology.2025.01.010","DOIUrl":"10.1016/j.theriogenology.2025.01.010","url":null,"abstract":"<div><div>Pseudopregnancy is the development of signs of pregnancy in the absence of an embryo or fetus. The objectives of this study were to characterize pseudopregnancy in dromedary camels, determine its prevalence in camel farms and practice, identify associated risk factors, and describe its clinical and hormonal properties. The prevalence of pseudopregnancy on 100 camel farms with 4264 total female camels was determined to be 2.07 % (86/4264) overall, while the rate among infertile animals was 17.68 % (1385/7833). The genital tracts of 58 pseudopregnant female camels were examined, and their breeding histories were examined. Serum concentrations of estradiol-17 β (E2), progesterone (P4), and prolactin (PRL) in these animals were assessed. Five cyclic camels and five in early pregnancy were used as control subjects. Signs of pseudopregnancy included being anestrous, refusing to mate, and adopting a stiffened posture—with the head held high and the tail cocked—when approached by a male. Normal pregnancy-associated mammary and abdominal changes were absent. Risk factors associated with pseudopregnancy included age (odds ratio [OR] = 21.63, P = 0.0001) and a history of reproductive disorders (OR = 4.155, P = 0.042). Based on their P4 levels, the pseudopregnant camels were classified as either typical (high P4, 16/58, 27.59 %) or atypical (low P4, 42/58, 72.41 %). The main clinical findings in the camels with typical pseudopregnancies were a narrow/closed cervix (56.25 %), clinical endometritis (CE; 43.75 %), and pyometra (31.25 %), while those with atypical pseudopregnancies exhibited CE (50 %) and luteinized follicles (43.1 %). The pregnant camels had significantly (P = 0.0001) higher serum P4 concentrations (2.44 ± 0.32 ng/mL) than the pseudopregnant (0.68 ± 0.12 ng/mL) and cyclic camels (0.16 ± 0.01 ng/mL). Serum E2 levels did not differ significantly among the pseudopregnant (43.2 ± 1.05 pg/mL), pregnant (47.72 ± 4.06 pg/mL), and cyclic (40.72 ± 1.03 pg/mL) camels. The pregnant camels had a significantly (P = 0.04) higher average serum PRL concentration (3.61 ± 0.45 ng/mL) than the pseudopregnant (2.77 ± 0.12 ng/mL) and cyclic camels (2.18 ± 0.11). In conclusion, pseudopregnancy in camels exhibits the same external signs that characterize pregnancy, but pseudopregnancy involves an absence of edema of the udder, milk production, and high PRL levels. We propose a division of pseudopregnant camels into typical and atypical classes, depending on whether they have high P4 levels. Pseudopregnancy is associated with a high incidence of other reproductive disorders, particularly in older camels.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 196-202"},"PeriodicalIF":2.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproduction control in a feral feline population using an anti-GnRH vaccine","authors":"García María Florencia ,&nbsp;Nuñez Favre Romina ,&nbsp;Stornelli María Cecilia ,&nbsp;Garcia Mitacek María Carla ,&nbsp;Rearte Ramiro ,&nbsp;de la Sota Rodolfo Luzbel ,&nbsp;Stornelli María Alejandra","doi":"10.1016/j.theriogenology.2025.01.017","DOIUrl":"10.1016/j.theriogenology.2025.01.017","url":null,"abstract":"<div><div>This study aimed to evaluate the use of the Improvac® vaccine to avoid heat and pregnancies in queens and fertility in males during the breeding season. Twenty-eight intact animals were divided into treated males (G1, n = 7), treated females (G2, n = 18), and untreated males (G3, n = 3) that were untamed and could not be captured for immunization. In cats from the G1 group, the testicular volume (337.35 ± 95.74 mm³) and testosterone concentration (1.31 ± 0.49 ng/mL) reached the lowest value 16 weeks after the first vaccination. At week 40, all queens exhibited both estrus cytology and estrus behavior, with serum estrogen (38.5 ± 1.93 pg/mL) and progesterone (0.5 ng/mL) concentrations within the physiological range for the phase. Eleven queens received a third dose of the vaccine at week 40, and none became pregnant by week 64. The remaining queens (n = 7) did not receive the third dose of the vaccine and became pregnant by week 44. In cats from the G1 and G2 groups, the hematologic parameters were within the physiological range for the species. The results of this study indicate that the Improvac® vaccine is safe and effective in the short to medium term in preventing cat reproduction.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"235 ","pages":"Pages 203-209"},"PeriodicalIF":2.4,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}