Theriogenology最新文献_第4页

Reproductive efficiency of Bos indicus cows under different estradiol-free ovulation synchronization protocols 不同无雌二醇排卵同步方案下印度母牛的繁殖效率

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-13 DOI: 10.1016/j.theriogenology.2026.117830

Lucas Araujo Lemos , Laís Reis Carvalho , Matheus Pedroso Vicente , Luiz Manoel Souza Simões , Leonardo Silva Fernandes do Vale , José Camisão de Souza , José Nélio de Sousa Sales

The objective was to evaluate the fertility of Bos indicus cows submitted to an ovulation synchronization protocol without estrogen (E2). In experiment 1, injectable P4 (P4i) was administered 10 days (D-10) before the TAI protocol. On D0, cows received one intravaginal P4 device and estradiol benzoate (EB; Control) or buserelin acetate (GnRH/EC and 2GnRH). On D8, devices were removed, and all cows received eCG and PGF2α. Additionally, cows received estradiol cypionate (EC) on D8 (Control and GnRH/EC) or buserelin acetate on D10 (2GnRH). In Experiment 2, cows in the Control group underwent a protocol similar to Experiment 1. Cows in the GnRH/EC, 2GnRH/48h, and 2GnRH/54h groups were pre-synchronized with an intravaginal P4 device (D-7) and received gonadorelin and an intravaginal P4 device on D0. P4 devices were removed on D8, and all cows received eCG and PGF2α. Furthermore, the GnRH/EC group received EC, and cows in the 2GnRH/48h and 2GnRH/54h groups were administered GnRH 48 h (h) after removal of the P4 device. TAI was performed 48 (2GnRH/48h) or 54h (2GnRH/54h) after removal of the P4 device. In all experiments, the follicular diameter on D8 was greater in cows that received GnRH on D0 (P ≤ 0.01) as well as a greater ovulation rate on D0 (P ≤ 0.01). Furthermore, estrus expression was greater in cows receiving EC (P = 0.001; Exp. 2). P/AI was greater in the Control group (P = 0.001; Exp. 2). In conclusion, removal of estradiol in ovulation synchronization protocols resulted in lower fertility relative to E2-based controls.

目的是评估在没有雌激素（E2）的情况下接受排卵同步方案的波斯母牛的生育能力。实验1，在TAI方案前10天（D-10）注射P4 （P4i）。在第0天，奶牛接受1个阴道内P4装置和苯甲酸雌二醇（EB；对照）或醋酸布丝林（GnRH/EC和2GnRH）。D8时，移除设备，所有奶牛均接受心电图和PGF2α检测。此外，奶牛在D8（对照和GnRH/EC）饲喂雌二醇（EC），在D10 （2GnRH）饲喂醋酸布丝林（buserelin acetate）。在试验2中，对照组奶牛采用与试验1相似的处理方案。GnRH/EC组、2GnRH/48h组和2GnRH/54h组奶牛预先同步阴道内P4装置（D-7），并在D0时接受促性腺激素和阴道内P4装置。D8取下P4装置，所有奶牛均接受心电图和PGF2α检测。GnRH/EC组给予EC， 2GnRH/48h和2GnRH/54h组奶牛在取下P4装置48h (h)后给予GnRH。取下P4装置后48 （2GnRH/48h）或54 （2GnRH/54h）行TAI。在所有试验中，D0日接受GnRH的奶牛在D8日的卵泡直径更大（P≤0.01），D0日的排卵率更高（P≤0.01）。此外，接受EC的奶牛的发情表达更高（P = 0.001; Exp. 2）。对照组P/AI更大（P = 0.001; Exp. 2）。综上所述，与e2对照相比，在排卵同步方案中去除雌二醇导致了较低的生育能力。

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引用次数: 0

Spatial differences in the ovine cervical microenvironment at the follicular and luteal phases of the oestrous cycle; Implications for sperm transport 发情周期卵泡期和黄体期绵羊宫颈微环境的空间差异对精子运输的影响

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-13 DOI: 10.1016/j.theriogenology.2026.117828

N. Johnston , E. Bini , E. Fitzpatrick , C. Reid , S. Fair

The complex anatomy of the ovine cervix presents a significant barrier to transcervical artificial insemination, necessitating sperm deposition at the external cervical Os. This study aimed to evaluate the cervical microarchitecture to elucidate the mechanisms orchestrating sperm transport across the cervix. Cervices from crossbred ewes (n = 13) were harvested during the follicular (n = 6) and luteal (n = 7) phase, sectioned into 10 transverse segments from external to internal Os (1 = External cervical Os; 10 = Internal cervical Os), and analysed for epithelial morphology and microgroove architecture. Columnar epithelium predominated throughout the cervix, with a squamocolumnar junction localised at the external Os. Columnar cell height was significantly greater during the follicular phase (33.3 ± 1.31 μm) than the luteal phase (26.7 ± 1.21 μm; p < 0.001). Cervical microgroove surface area increased during the follicular phase (p < 0.05). Microgroove surface area, depth, and tortuosity (deviation from a straight line) increased progressively toward the internal Os and were enhanced during the follicular phase (p < 0.05). Sialic acid expression was elevated in secondary and tertiary microgrooves during the follicular phase (p < 0.001), with mucin-bound sialic acids showing spatial variation along the canal. Segment 4 exhibited the lowest sialic acid expression (p < 0.01). Reproductive phase by cervical segment interactions affected both the secondary, tertiary microgroove epithelium, and surrounding luminal region adjacent to the tertiary microgrooves (p < 0.001). This study provides a detailed characterisation of the microarchitecture of the ovine cervical canal which is critical to understanding the mechanisms orchestrating sperm transport.

绵羊子宫颈复杂的解剖结构为经宫颈人工授精提供了一个重要的障碍，需要精子在宫颈外o处沉积。本研究旨在评估子宫颈微结构，以阐明协调精子在子宫颈运输的机制。在卵泡期（n = 6）和黄体期（n = 7）采集杂交母羊（n = 13）的宫颈，从外颈到内颈（1 =外颈o； 10 =内颈o）横切成10个横切段，分析上皮形态和微槽结构。柱状上皮主要分布于整个子宫颈，鳞状-柱状连接位于子宫颈外o区。卵泡期柱状细胞高度（33.3±1.31 μm）显著高于黄体期（26.7±1.21 μm; p < 0.001）。卵泡期宫颈微沟表面积增大（p < 0.05）。微沟槽表面积、深度和弯曲度（偏离直线）向内部o逐渐增加，并在卵泡期增强（p < 0.05）。在卵泡期，唾液酸在二级和三级微槽中的表达升高（p < 0.001），黏液结合的唾液酸在管道中表现出空间差异。第4段唾液酸表达量最低（p < 0.01）。生殖期宫颈节段相互作用影响了二级、三级微槽上皮以及邻近三级微槽的周围腔区（p < 0.001）。本研究详细描述了羊宫颈管的微结构，这对理解协调精子运输的机制至关重要。

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引用次数: 0

Comparative post-breeding outcomes in jennies inseminated with cryopreserved semen re-extended in seminal plasma or treated with platelet-rich plasma 用冷冻保存的精液受精或用富血小板血浆处理的珍妮受精后的比较结果

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-13 DOI: 10.1016/j.theriogenology.2026.117832

Carla Joseph , Holly Schwarzman , Claire Kaplan , Marcus Machado , Bruno Trevisan , Lorenzo Segabinazzi

Artificial insemination (AI) with cryopreserved semen in donkeys is challenging due to poor fertility, likely caused by an exacerbated post-breeding inflammatory response. This study evaluated the effects of frozen-thawed semen re-extension in seminal plasma (SP) and intrauterine infusion of platelet-rich plasma (PRP) on post-breeding uterine inflammation, progesterone concentrations, and fertility in jennies. A total of 68 estrous cycles from 14 fertile jennies were randomly assigned to one of five groups: insemination with frozen-thawed semen reconstituted in 7 mL of SP (SP, n = 12); insemination with frozen-thawed semen followed by intrauterine infusion of Lactated Ringer's Solution (LRS) (Control, n = 14) or autologous PRP (PRP, n = 14) 6h post-AI; insemination with fresh semen (FS, n = 14); and sham-insemination with LRS following PRP infusion 6h post-AI (PRP.only, n = 14). Uterine lavage was performed 6h post-AI in all cycles. Intrauterine fluid accumulation (IUF), endometrial neutrophil counts (PMN), corpus luteum (CL) volume, and plasma progesterone concentration were assessed multiple times before and after AI. Pregnancy diagnosis was performed on Day 14. FS and SP groups had lower neutrophil counts 6h post-AI than Control and PRP groups (P < 0.05). PRP.only cycles had the lowest neutrophil counts at 6h and 24h post-AI (P < 0.05). Neutrophil counts were similar between all groups at 48h post-AI (P > 0.05). Plasma progesterone was higher in FS cycles on Days 3 and 8 compared to Control-assigned cycles (P < 0.05), and Day 14 compared to all groups (P < 0.05). CL volume and IUF were similar across groups (P > 0.05). Pregnancy rates were higher in FS cycles (71 %) compared to all other groups (Control, 0 %; PRP, 14 %; SP, 8 %; P < 0.05). In conclusion, SP reduced post-breeding inflammation but did not improve fertility outcomes in jennies AI with cryopreserved semen, while PRP had no effect. Additionally, plasma progesterone was affected by the type of semen used for AI but not by treatments.

由于驴的生育能力差，可能是由于繁殖后炎症反应加剧造成的，因此用低温保存的精液进行人工授精（AI）是具有挑战性的。本研究评估了冷冻解冻精液在精浆中再延伸（SP）和宫内输注富血小板血浆（PRP）对育龄后子宫炎症、孕酮浓度和生育力的影响。选取14只可育雌犬68个发情周期，随机分为5组：用7 mL SP （SP, n = 12）重组的冻融精液进行人工授精；人工授精后6h，用冻融精液授精，然后宫内输注乳酸林格液（LRS）（对照组，n = 14）或自体PRP (PRP, n = 14)；新鲜精液授精（FS, n = 14）；人工智能（PRP）后6小时注射PRP后LRS假授精。只有，n = 14)。各周期术后6h进行子宫灌洗。多次评估人工智能前后宫内积液（IUF）、子宫内膜中性粒细胞计数（PMN）、黄体（CL）体积、血浆黄体酮浓度。第14天进行妊娠诊断。人工智能后6h， FS组和SP组中性粒细胞计数低于对照组和PRP组（P < 0.05）。PRP。只有周期在ai后6h和24h中性粒细胞计数最低（P < 0.05）。术后48h各组中性粒细胞计数相近（P > 0.05）。与对照组相比，FS周期第3天和第8天血浆黄体酮水平较高（P < 0.05），第14天血浆黄体酮水平与所有组相比均较高（P < 0.05）。各组间CL体积和IUF相似（P > 0.05）。与所有其他组相比，FS周期的妊娠率（71%）更高（对照组，0%；PRP, 14%； SP, 8%; P < 0.05）。综上所述，SP减少了繁殖后的炎症，但没有改善冷冻保存精液的珍妮犬的生育结果，而PRP没有影响。此外，血浆黄体酮受用于人工智能的精液类型的影响，但不受治疗方法的影响。

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引用次数: 0

Telomere lengths in blood and sperm as biomarkers of reproductive aging and semen quality in dogs 血液和精子端粒长度作为犬生殖衰老和精液质量的生物标志物。

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-12 DOI: 10.1016/j.theriogenology.2026.117827

Chiara Del Prete , Alessandra Iannuzzi , Valentina Longobardi , Maria Pia Pasolini , Ramona Pistucci , Alfonso Calabria , Bianca Gasparrini , Natascia Cocchia

To assess the potential utility of leukocytes and spermatozoa telomere length (LTL and STL) as reproductive biomarkers, this study measured both LTL and STL and investigated their possible correlations with oxidative status and semen quality parameters in healthy breeding male dogs. Twenty-two ejaculates and blood samples were collected from dogs of various breeds. Semen was evaluated for volume, concentration, sperm motility, and kinetic parameters using a Sperm Class Analyzer. LTL and STL were quantified using Quantitative PCR (qPCR) and expressed as the relative ratio of telomere repeat copy number (T) to a single-copy gene (S), T/S ratio. Serum oxidative stress markers were assessed using d-ROMs and Biological Antioxidant Potential (BAP) tests. Correlations between STL and LTL, as well as their association with age, semen parameters and oxidative stress levels, were evaluated using Spearman analysis. LTL and STL were 0.63 ± 0.25 and 0.85 ± 0.31 and showed a strong positive correlation (P < 0.001; r_s = 0.70). Both LTL and STL were also negatively correlated with age (P < 0.05 and rs = −0.50; P < 0.001 and rs = −0.68). The cut-off age for the difference in STL and LTL was identified at 6 and 7.5 years, respectively. STL was also positively correlated with semen volume (P < 0.05; r_s = 0.63) and concentration (P < 0.05; r_s = 0.41) and negatively correlated with semen chromatin decondensation (P < 0.01; r_s = -0.71); while the LTL showed a positive correlation with sperm concentration (P < 0.05; r_s = 0.53). No correlations with oxidative markers were found. These findings support the potential use of TL as a biomarker for reproductive aging and semen quality in dogs.

为了评估白细胞和精子端粒长度（LTL和STL）作为生殖生物标志物的潜在效用，本研究测量了健康繁殖雄性犬的LTL和STL，并研究了它们与氧化状态和精液质量参数的可能相关性。从不同品种的狗身上收集了22份射精和血液样本。使用精子类分析仪评估精液的体积、浓度、精子活力和动力学参数。LTL和STL采用定量PCR （qPCR）进行定量，用端粒重复拷贝数(T)与单拷贝基因(S)的相对比值、T/S比值表示。采用d- rom和生物抗氧化潜能（BAP）试验评估血清氧化应激标志物。使用Spearman分析评估STL和LTL之间的相关性，以及它们与年龄、精液参数和氧化应激水平的关系。LTL与STL分别为0.63±0.25和0.85±0.31，呈强正相关（P = 0.70）。LTL和STL与年龄（P = 0.63）、浓度（P = 0.41）呈负相关，与精液染色质去浓缩（P = -0.71）呈负相关；LTL与精子浓度呈正相关（P = 0.53）。未发现与氧化标志物相关。这些发现支持TL作为犬生殖衰老和精液质量的生物标志物的潜在应用。

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引用次数: 0

SB216763 enhances proliferation and sustains the undifferentiated state of porcine spermatogonial cells in prolonged In vitro culture SB216763在猪精原细胞长时间体外培养中促进细胞增殖，维持未分化状态

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-08 DOI: 10.1016/j.theriogenology.2026.117816

Benliang Zhou , Zhen Zhang , Anqin Duan , Jiaxiang Ji , Mengqi Li , Rui Chen , Xiangxing Zhu , Jianghua Shang , Xiaogan Yang , Xingwei Liang

Spermatogonial stem cells (SSCs) serve as the foundation of spermatogenesis and play a critical role in livestock fertility and genetic improvement. However, maintaining these cells in long-term culture remains challenging, especially in pigs, where preserving both proliferative capacity and the undifferentiated state has proven difficult. To overcome this limitation, we investigated SB216763 (SB), a selective GSK-3β inhibitor reported to enhance proliferation in other stem cell types, for its potential effects on porcine spermatogonial cells (pSCs). In a 9-day concentration screening (0, 1.25, 2.5, and 5 μM SB), SB treatment significantly enhanced the proliferation of pSCs. It upregulated the proliferation-related gene PCNA and the antiapoptotic gene BCL2, while downregulating key apoptosis-related genes, including BAX and Caspase-3. It also promoted the expression of DAZL, UCHL1, NANOS2, and OCT4. Furthermore, over a 55-day period, treatment with 2.5 μM SB consistently supported higher proliferation rates, a larger proportion of DAZL-positive cells, and elevated expression of undifferentiation-associated markers (NANOS2, OCT4) compared with untreated controls at each passage. Overall, our results demonstrate that SB216763 not only promotes the proliferation of pSCs but also helps maintain their germline identity, offering a feasible strategy to improve the long-term in vitro culture of porcine spermatogonial cells.

精原干细胞（SSCs）是精子发生的基础，在家畜生育和遗传改良中发挥着重要作用。然而，在长期培养中维持这些细胞仍然具有挑战性，特别是在猪中，保持增殖能力和未分化状态已被证明是困难的。为了克服这一限制，我们研究了SB216763 (SB)，一种选择性GSK-3β抑制剂，据报道可以增强其他干细胞类型的增殖，因为它对猪精原细胞（pSCs）有潜在的影响。在为期9天的浓度筛选（0、1.25、2.5和5 μM SB）中，SB处理显著增强了psc的增殖。上调增殖相关基因PCNA和抗凋亡基因BCL2，下调BAX和Caspase-3等关键凋亡相关基因。同时促进DAZL、UCHL1、NANOS2、OCT4的表达。此外，在55天的时间内，与未处理的对照组相比，2.5 μM SB处理始终支持更高的增殖率，更大比例的dazl阳性细胞，以及非分化相关标志物（NANOS2, OCT4）的表达升高。总之，我们的研究结果表明，SB216763不仅促进了psc的增殖，而且有助于维持其种系特性，为改善猪精原细胞的长期体外培养提供了一种可行的策略。

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引用次数: 0

Oleanolic acid prevents ferroptosis and enhances oocyte competence during in vitro maturation in a porcine model 齐墩果酸在猪模型的体外成熟过程中防止铁下垂并提高卵母细胞的能力

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-08 DOI: 10.1016/j.theriogenology.2026.117817

Xiang Zhang , Kukbin Ji , Hoyong Choi , Jaehyeok Yoon , Junghui Jo , Seonga Park , Chuang Li , Ye Liu , Inchul Choi , Min Kyu Kim

The in vitro production of porcine embryos is a valuable model for livestock biotechnology and human reproductive research. However, oocyte quality is often compromised during culture by ferroptosis, which is an iron-dependent form of cell death driven by lipid peroxidation and redox imbalance. This study induced ferroptosis using RSL3 during porcine oocyte maturation to evaluate the protective potential of oleanolic acid (OA), an antioxidant triterpenoid compound. Exposure to RSL3 increased Fe²⁺ accumulation (as detected by FerroOrange staining), lipid peroxidation (as determined by the MDA assay), apoptosis, and mitochondrial dysfunction. These changes were consistent with ferroptotic stress and were accompanied by impaired cumulus expansion, meiotic progression, and blastocyst formation. Supplementing with 1 mg/L OA significantly improved oocyte competence, partially restored blastocyst development, enhanced cumulus expansion, and reduced apoptosis. At the cellular level, OA reduced ROS, MDA, and abnormal mitochondrial distribution while maintaining glutathione levels and mitochondrial membrane potential. Molecular analysis revealed that OA modulated ferroptosis-related gene expression, including partial restoration of GPX4 and SLC7A11 expression and suppression of ACSL4 and TFRC upregulation. Collectively, these findings indicate that OA partially alleviated RSL3-induced ferroptotic damage in porcine oocytes, as reflected by coordinated improvements in iron homeostasis, redox status, mitochondrial function, and the expression of ferroptosis- and autophagy-associated markers. OA could therefore represent a promising technique to improve oocyte quality and embryonic development, with potential application value in reproductive medicine and livestock biotechnology.

猪胚胎的体外生产是家畜生物技术和人类生殖研究的一个有价值的模式。然而，在培养过程中，卵母细胞质量经常受到铁凋亡的影响，铁凋亡是一种由脂质过氧化和氧化还原失衡驱动的铁依赖性细胞死亡形式。本研究利用RSL3诱导猪卵母细胞成熟过程中的铁凋亡，以评估齐齐果酸（OA）（一种抗氧化的三萜化合物）的保护潜力。暴露于RSL3会增加Fe2+积累（通过铁橙色染色检测）、脂质过氧化（通过MDA测定）、细胞凋亡和线粒体功能障碍。这些变化与紧铁应激一致，并伴有积云扩张、减数分裂进程和囊胚形成受损。添加1 mg/L OA可显著提高卵母细胞能力，部分恢复囊胚发育，增强卵丘扩张，减少细胞凋亡。在细胞水平上，OA降低ROS、MDA和线粒体异常分布，同时维持谷胱甘肽水平和线粒体膜电位。分子分析表明，OA调节了铁中毒相关基因的表达，包括部分恢复GPX4和SLC7A11的表达，抑制ACSL4和TFRC的上调。综上所述，这些发现表明OA部分缓解了rsl3诱导的猪卵母细胞铁凋亡损伤，这可以通过铁稳态、氧化还原状态、线粒体功能以及铁凋亡和自噬相关标记物表达的协同改善来反映。因此，OA在提高卵母细胞质量和胚胎发育方面是一种很有前景的技术，在生殖医学和家畜生物技术方面具有潜在的应用价值。

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引用次数: 0

LH promotes testosterone synthesis in rooster leydig cells through YAP1/ACSL4/SOAT1 pathway LH通过YAP1/ACSL4/SOAT1途径促进公鸡leydig细胞的睾酮合成。

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-08 DOI: 10.1016/j.theriogenology.2026.117813

Ziming Wang , Jingxin Pu , Wen An , Chunqi Hou , Qiuju Zheng , Jing Zhao , Xin Ma , Yi Fang , Hongyu Liu , Jing Guo , He Ding , Yang Lyu , Jun Wang , Wenfa Lyu

Testosterone synthesis in Leydig cells requires precise coordination between LH signaling and cholesterol metabolism, but the mechanisms underlying this regulation remain incompletely understood. In this study, we reveal a previously unrecognized YAP1-ACSL4 axis essential for LH-induced testosterone production by integrating transcriptomic profiling and functional studies in Leydig cells. Transcriptomic sequencing identified ACSL4 and YAP1 as key regulators of testosterone synthesis induced by LH. The inhibition of YAP1 led to the downregulation of ACSL4 expression, which significantly reduced testosterone production. Similarly, si-ACSL4 directly reduced testosterone production in Leydig cells. Notably, transcriptomic sequencing after interfering with ACSL4 showed that SOAT1 is a key molecule downstream of ACSL4. SOAT1 promoted testosterone synthesis by inhibiting esterification of free cholesterol and subsequently increasing cholesterol availability providing materials for testosterone synthesis. Mechanistically, ACSL4 modulates cholesterol availability through SOAT1 suppression, shifting metabolic flux from cholesterol ester storage toward cholesterol. These results indicate that LH promotes testosterone synthesis by upregulating YAP1 expression, which in turn increases ACSL4 expression, inhibits SOAT1 expression, and raises the concentration of free cholesterol. This finding provides the first evidence linking ACSL4-mediated lipid metabolism to gonadotropin-regulated steroidogenesis.

间质细胞的睾酮合成需要LH信号和胆固醇代谢之间的精确协调，但这种调节的机制尚不完全清楚。在这项研究中，我们通过整合Leydig细胞的转录组学分析和功能研究，揭示了以前未被识别的YAP1-ACSL4轴对lh诱导的睾酮产生至关重要。转录组测序发现ACSL4和YAP1是LH诱导的睾酮合成的关键调节因子。抑制YAP1导致ACSL4表达下调，从而显著降低睾酮的产生。同样，si-ACSL4直接减少间质细胞的睾酮分泌。值得注意的是，干扰ACSL4后的转录组测序显示SOAT1是ACSL4下游的关键分子。SOAT1通过抑制游离胆固醇的酯化反应促进睾酮合成，随后增加胆固醇的可用性，为睾酮合成提供材料。从机制上讲，ACSL4通过抑制SOAT1调节胆固醇可用性，将代谢通量从胆固醇酯储存转移到胆固醇。这些结果表明，LH通过上调YAP1表达促进睾酮合成，进而增加ACSL4表达，抑制SOAT1表达，提高游离胆固醇浓度。这一发现提供了将acsl4介导的脂质代谢与促性腺激素调节的类固醇生成联系起来的第一个证据。

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引用次数: 0

Staying alive: a questionnaire study on pregnancy outcome and pre-weaning puppy health in Swedish dogs 存活：瑞典犬妊娠结局和断奶前幼犬健康的问卷调查研究

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-07 DOI: 10.1016/j.theriogenology.2026.117814

Anna Bonnevie , Karolina Engdahl , Emelie Pettersson , Helene Alm , Ingrid Hansson , Bodil Ström Holst

Pre-weaning mortality and stillbirth rates in dogs are relatively high. Hypoxia during prolonged parturition is the main cause of neonatal death, followed by septic infection, predominantly caused by opportunistic bacteria. Several viruses also contribute to neonate mortality. Good breeding management and vaccination are important in improving newborn survival. The purpose of the study was to establish pregnancy outcome rates in a large cohort of Swedish breeding dogs, information that had not been previously documented. Further, we aimed to identify risk factors associated with abortion, congenital anomalities, and puppy mortality. Last, we wanted to investigate potential risk for transmission of Brucella canis or other zoonoses related to parturition. To achieve this, a web-based questionnaire was distributed to dog breeders in the Swedish Kennel Club.

The results comprise data from 1879 breeders on 10,124 puppies from 1791 litters. Abortion rate was 1.5 %, stillbirth rate 4.8 %, and pre-weaning mortality 3.7 %. Disease in the pregnant bitch was associated with spontaneous abortion or resorption. Stillbirth was associated with the bitch having her first litter, dystocia, increased litter size and presence of congential anomalities in the litter. Pre-weaning mortality was associated with dystocia and increased litter size. Having seven or more dogs less than one year old together with the bitch during the last weeks of pregnancy was a risk factor for both stillbirth and pre-weaning mortality, an association not previously shown. Thirty per cent of respondents reported having used mouth-to-nose resuscitation on newborn puppies, a potential transmit route for Brucella canis and other zoonotic agents.

狗的断奶前死亡率和死产率相对较高。长时间分娩期间缺氧是新生儿死亡的主要原因，其次是脓毒性感染，主要由机会性细菌引起。有几种病毒也会导致新生儿死亡。良好的育种管理和疫苗接种对提高新生儿存活率很重要。这项研究的目的是确定一大批瑞典繁殖犬的妊娠结局率，这是以前没有文献记载的信息。此外，我们旨在确定与流产、先天性异常和幼犬死亡率相关的危险因素。最后，我们希望调查与分娩有关的犬布鲁氏菌或其他人畜共患疾病传播的潜在风险。为此，向瑞典养犬俱乐部的狗饲养员分发了一份基于网络的调查问卷。研究结果包括1879名饲养员对1791窝幼犬的10124只幼犬的数据。流产率1.5%，死产率4.8%，断奶前死亡率3.7%。怀孕母狗的疾病与自然流产或吸收有关。死产与母狗有她的第一胎，难产，产仔数量增加和产仔中存在先天性异常有关。断奶前死亡率与难产和产仔数增加有关。在怀孕的最后几周，有七只或更多不到一岁的狗和母狗在一起是死产和断奶前死亡的一个危险因素，这一关联之前没有被证明。30%的答复者报告曾对新生幼犬进行口鼻复苏，这是犬布鲁氏菌和其他人畜共患病原体的潜在传播途径。

{"title":"Staying alive: a questionnaire study on pregnancy outcome and pre-weaning puppy health in Swedish dogs","authors":"Anna Bonnevie , Karolina Engdahl , Emelie Pettersson , Helene Alm , Ingrid Hansson , Bodil Ström Holst","doi":"10.1016/j.theriogenology.2026.117814","DOIUrl":"10.1016/j.theriogenology.2026.117814","url":null,"abstract":"<div><div>Pre-weaning mortality and stillbirth rates in dogs are relatively high. Hypoxia during prolonged parturition is the main cause of neonatal death, followed by septic infection, predominantly caused by opportunistic bacteria. Several viruses also contribute to neonate mortality. Good breeding management and vaccination are important in improving newborn survival. The purpose of the study was to establish pregnancy outcome rates in a large cohort of Swedish breeding dogs, information that had not been previously documented. Further, we aimed to identify risk factors associated with abortion, congenital anomalities, and puppy mortality. Last, we wanted to investigate potential risk for transmission of <em>Brucella canis</em> or other zoonoses related to parturition. To achieve this, a web-based questionnaire was distributed to dog breeders in the Swedish Kennel Club.</div><div>The results comprise data from 1879 breeders on 10,124 puppies from 1791 litters. Abortion rate was 1.5 %, stillbirth rate 4.8 %, and pre-weaning mortality 3.7 %. Disease in the pregnant bitch was associated with spontaneous abortion or resorption. Stillbirth was associated with the bitch having her first litter, dystocia, increased litter size and presence of congential anomalities in the litter. Pre-weaning mortality was associated with dystocia and increased litter size. Having seven or more dogs less than one year old together with the bitch during the last weeks of pregnancy was a risk factor for both stillbirth and pre-weaning mortality, an association not previously shown. Thirty per cent of respondents reported having used mouth-to-nose resuscitation on newborn puppies, a potential transmit route for <em>Brucella canis</em> and other zoonotic agents.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"254 ","pages":"Article 117814"},"PeriodicalIF":2.5,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of osteopontin treatment alone and in combination with hormonal therapy on endometrial epidermal growth factor concentration and fertility at the first insemination after calving in dairy cows 骨桥蛋白单独和联合激素治疗对奶牛产犊后首次授精子宫内膜表皮生长因子浓度和生育力的影响。

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-05 DOI: 10.1016/j.theriogenology.2026.117812

Taiwo Kamardeen Bello , Takashi Tanida , Takayoshi Tagami , Yojiro Yanagawa , Seiji Katagiri

Delayed recovery of cyclic changes in epidermal growth factor (EGF) concentration in the endometrium is related to low conception rates at the first insemination following parturition. An intravaginal infusion of osteopontin (OPN) normalizes EGF profile and improves fertility in repeat-breeder (RB) cows. Therefore, the present study determined whether this treatment can restore normal EGF profile in postpartum cows and improve fertility. First, the effects of recombinant OPN (rOPN) and OPN peptide were examined. In cows with abnormal EGF profile, treatment with both rOPN and OPN peptides increased the normalization rate of EGF profile (78.3 % and 53.1 %, respectively) and conception rates (47.8 % and 61.2 %, respectively), with the rates being higher than those of the control group (25.0 % and 20.0 %, respectively). Second, the effects of combined OPN and hormonal therapy, an established treatment for this abnormality in RB cows, were investigated. Cows were treated with OPN peptide (320 nmol or 1600 nmol) alone and in combination with hormonal therapy. The combined treatment significantly increased EGF normalization and conception rates than the peptide alone (40.5 % vs. 21.9 % and 35.1 % vs. 19.0 %, respectively). The combined treatment achieved the highest conception rate among all groups, including those that received 1600 nmol OPN peptide with or without hormonal therapy. These results indicated that although OPN peptide alone improves fertility, combining it with hormonal therapy could be a better option in cows at the first insemination following parturition.

子宫内膜中表皮生长因子（EGF）浓度周期性变化的延迟恢复与分娩后第一次人工授精的低受孕率有关。阴道内灌注骨桥蛋白（OPN）可使EGF谱正常化并提高重复繁殖奶牛（RB）的生育能力。因此，本研究确定这种处理是否可以恢复产后奶牛正常的EGF水平并提高生育能力。首先，研究了重组OPN （rOPN）和OPN肽的作用。在EGF异常奶牛中，同时使用rOPN和OPN肽可使EGF正常率（分别为78.3%和53.1%）和受孕率（分别为47.8%和61.2%）提高，高于对照组（分别为25.0%和20.0%）。其次，研究了OPN和激素联合治疗（一种针对RB奶牛这种异常的既定治疗方法）的效果。奶牛分别接受320 nmol或1600 nmol的OPN肽单独和激素联合治疗。联合治疗显著提高EGF正常化和受孕率（分别为40.5%比21.9%和35.1%比19.0%）。综合治疗的受孕率在所有组中最高，包括那些接受1600 nmol OPN肽加或不加激素治疗的组。这些结果表明，虽然OPN肽单独使用可以提高奶牛的生育能力，但在奶牛分娩后第一次授精时，将其与激素治疗相结合可能是更好的选择。

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引用次数: 0

Interspecific SCNT in yak followed by embryo aggregation improves early in vitro developmental parameters without restoring pluripotent gene expression 牦牛种间SCNT后的胚胎聚集改善了早期体外发育参数，但不恢复多能基因的表达

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology

Pub Date : 2026-01-02 DOI: 10.1016/j.theriogenology.2026.117811

M. Yauri Felipe , M. Duque Rodriguez , A. De Stefano , L.D. Ratner , V. Alberio , D.F. Salamone

The efficiency of interspecific somatic cell nuclear transfer (iSCNT) remains suboptimal, necessitating novel approaches. This study aimed to evaluate the effect of embryo aggregation on the in vitro development of yak clones produced by iSCNT. Zona free cloned embryos were generated using male yak somatic cells and cultured either individually (Y1x, B1x group) or in pairs (Y2x group) in microwells. Aggregation improved the in vitro development of cloned yak embryos, yielding a blastocyst rate of 40.38 % in the Y2x group, which is significantly higher than the 25.34 % and 18.24 % exhibited in Y1x and B1x groups, respectively (P < 0.05), and comparable to the 48.14 % in the IVF control group. Gene expression analysis demonstrated that Y1x blastocysts exhibited significantly lower CDX2 and NANOG levels than those in the Y2x, B1x, and IVF groups. OCT4 levels expression did not differ between groups, and SOX2 levels expression was similar in Y1x, Y2x, and IVF. Y2x embryos showed a higher total cell number than Y1x, though still lower than IVF. In addition, Y2x embryos exhibited fewer CDX2⁺, OCT4⁺, and SOX2⁺ cells compared to B1x and IVF groups. Embryo transfers were performed as a preliminary evaluation of the developmental potential of aggregated embryos. Although early pregnancy signals were detected exclusively in the Y2x group (13 %; 3/25), no embryos were detected at day 42 of gestation. In conclusion, embryo aggregation in yak iSCNT embryos improves selected early in vitro developmental parameters and blastocyst morphology, but does not restore all developmentally relevant gene expression, nor sustain post-implantation development.

种间体细胞核移植（iSCNT）的效率仍然不理想，需要新的方法。本研究旨在探讨胚胎聚集对体外培养牦牛体细胞克隆发育的影响。利用公牦牛体细胞制备无带克隆胚胎，分别在微孔中单独培养（Y1x、B1x组）或成对培养（Y2x组）。聚集促进了克隆牦牛胚胎的体外发育，Y2x组囊胚率为40.38%，显著高于Y1x组的25.34%和B1x组的18.24% (P < 0.05)，与体外受精对照组的48.14%相当。基因表达分析表明，与Y2x、B1x和IVF组相比，Y1x囊胚的CDX2和NANOG水平明显降低。各组间OCT4水平表达无差异，而SOX2水平表达在Y1x、Y2x和IVF中相似。Y2x胚胎的细胞总数高于Y1x，但仍低于IVF。此外，与B1x和IVF组相比，Y2x胚胎显示出较少的CDX2+、OCT4+和SOX2+细胞。胚胎移植是对聚集胚胎发育潜力的初步评估。虽然Y2x组只检测到早期妊娠信号（13%;3/25），但妊娠第42天未检测到胚胎。综上所述，牦牛iSCNT胚胎中的胚胎聚集改善了体外早期发育参数和囊胚形态，但不能恢复所有发育相关基因的表达，也不能维持着床后的发育。

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引用次数: 0