Pub Date : 2022-01-01DOI: 10.53388/mhm2021p1204001
Yang-qing Zhan, Ruifeng Chen, Q. Ma, Jinping Zheng, Xilong Deng, Wei Yang, L. Fu, Nanshan Zhong, Zifeng Yang
{"title":"Efficacy and safety of Phillyrin (KD-1) capsule in the treatment of moderate COVID-19: protocol for a randomized controlled trial","authors":"Yang-qing Zhan, Ruifeng Chen, Q. Ma, Jinping Zheng, Xilong Deng, Wei Yang, L. Fu, Nanshan Zhong, Zifeng Yang","doi":"10.53388/mhm2021p1204001","DOIUrl":"https://doi.org/10.53388/mhm2021p1204001","url":null,"abstract":"","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80513603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.53388/mhm2022b0825001
Tao Xue, Shuai Gong, Tian-Hao Xie, Wenjuan Li, Jianyin Huang
{"title":"Development status and trend of traditional Chinese medicine database","authors":"Tao Xue, Shuai Gong, Tian-Hao Xie, Wenjuan Li, Jianyin Huang","doi":"10.53388/mhm2022b0825001","DOIUrl":"https://doi.org/10.53388/mhm2022b0825001","url":null,"abstract":"","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86962837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.53388/mhm2022b0507001
Herong Cui, Jiaxin Zheng, Xiaoyu Zhang, H. Shang
{"title":"Prevention and treatment of traditional Chinese medicine against angiogenesis related cardiovascular diseases based on translational medicine","authors":"Herong Cui, Jiaxin Zheng, Xiaoyu Zhang, H. Shang","doi":"10.53388/mhm2022b0507001","DOIUrl":"https://doi.org/10.53388/mhm2022b0507001","url":null,"abstract":"","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78549631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.53388/mhm2022a0108001
R. Sun, Jie Xu, Yanyu Zhang, Xiaoqi Sun, X. Cui
Objective To observe the effects of Guizhi Fuling capsule (GZFLC) on RPMI 8226 cells and explore the mechanisms. Methods Cell Counting Kit-8 (CCK-8) assays and flow cytometry were used to detect the viability and apoptosis levels of RPMI 8226 cells. The effects on mitochondria were examined by ROS and JC-1 assays. Western blotting was used to detect the expression of B cell lymphoma-2 (Bcl-2), Bax, cleaved caspase-3, and Apoptotic protease-activating factor 1 (Apaf-1). Results GZFLC drug serum decreased the viability and increased the apoptosis of RPMI 8226 cells. In addition, this drug increased ROS levels and decreased the mitochondrial membrane potential (MMP). Western blotting showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups, whereas the expression levels of cleaved caspase-3 and Apaf-1 were increased. Conclusion GZFLC promoted apoptosis of myeloma cells through the mitochondrial apoptosis pathway.
{"title":"Guizhi Fuling capsule can induce apoptosis of myeloma cells through the mitochondrial apoptosis pathway","authors":"R. Sun, Jie Xu, Yanyu Zhang, Xiaoqi Sun, X. Cui","doi":"10.53388/mhm2022a0108001","DOIUrl":"https://doi.org/10.53388/mhm2022a0108001","url":null,"abstract":"Objective To observe the effects of Guizhi Fuling capsule (GZFLC) on RPMI 8226 cells and explore the mechanisms. Methods Cell Counting Kit-8 (CCK-8) assays and flow cytometry were used to detect the viability and apoptosis levels of RPMI 8226 cells. The effects on mitochondria were examined by ROS and JC-1 assays. Western blotting was used to detect the expression of B cell lymphoma-2 (Bcl-2), Bax, cleaved caspase-3, and Apoptotic protease-activating factor 1 (Apaf-1). Results GZFLC drug serum decreased the viability and increased the apoptosis of RPMI 8226 cells. In addition, this drug increased ROS levels and decreased the mitochondrial membrane potential (MMP). Western blotting showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups, whereas the expression levels of cleaved caspase-3 and Apaf-1 were increased. Conclusion GZFLC promoted apoptosis of myeloma cells through the mitochondrial apoptosis pathway.","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86800619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.53388/mhm2022a1018001
Rui Zheng, Zhao Chen, Chen Zhao, Xiaoyu Zhang, Mingli Ma, Yang Sun, H. Shang
{"title":"Thinking and practice on clinical safety evaluation of combination of traditional Chinese medicine and western medicine","authors":"Rui Zheng, Zhao Chen, Chen Zhao, Xiaoyu Zhang, Mingli Ma, Yang Sun, H. Shang","doi":"10.53388/mhm2022a1018001","DOIUrl":"https://doi.org/10.53388/mhm2022a1018001","url":null,"abstract":"","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90577054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-09DOI: 10.21203/RS.3.RS-390546/V1
Xiuping Kuang, Yingnan Jiang, Jiwei Huang, Yongzhi Guo, weixi Li
Background Indirubin, isolated from Indigo Naturals, is reported to have the inhibitory activity of MCF-7 human breast cancer cells in vitro. However, studies on its anti-breast cancer activity in vivo and underlying mechanism are insufficient. We explored whether indirubin could trigger ferroptosis of breast cancer cells to exert anti-tumor activity. Methods Bioinformatical analysis was performed to detected the expression of prostaglandin-endoperoxide synthase 2 (Ptgs2) in breast cancer tissues Ptgs2-related prognosis for patients with breast cancer. Growth of 4T1 cells was assessed using wound healing assay and MTT assay. The levels of 4-HNE, GPX4, PTGS2 and GSK-3β proteins were detected by Western blot, and the mRNA of Ptgs2 was tested by qPCR. The GSH and MDA were determined by commercial kits. Molecular docking was employed to study interaction between indirubin and GSK-3β. An 4T1 murine breast cancer was adopted to evaluate the in vivo antitumor activity of indirubin. Results Indirubin promoted ferroptosis of 4T1 breast cancer cells with deplete of GSH, increased MDA and 4-HNE level, as well as decreased GPX4 expression. Indirubin suppressed the growth of 4T1 breast tumor in vivo. Mechanism study showed indirubin up regulated Ptgs2 expression by promoting phosphorylation (Ser 9) of GSK-3β. Conclusions Indirubin suppresses 4T1 murine breast cancer in vitro and in vivo by induction of ferroptosis and up-regulation of Ptgs2.
{"title":"Indirubin suppresses 4T1 murine breast cancer in vitro and in vivo by induction of ferroptosis and up-regulation of Ptgs2","authors":"Xiuping Kuang, Yingnan Jiang, Jiwei Huang, Yongzhi Guo, weixi Li","doi":"10.21203/RS.3.RS-390546/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-390546/V1","url":null,"abstract":"\u0000 Background\u0000\u0000Indirubin, isolated from Indigo Naturals, is reported to have the inhibitory activity of MCF-7 human breast cancer cells in vitro. However, studies on its anti-breast cancer activity in vivo and underlying mechanism are insufficient. We explored whether indirubin could trigger ferroptosis of breast cancer cells to exert anti-tumor activity.\u0000Methods\u0000\u0000Bioinformatical analysis was performed to detected the expression of prostaglandin-endoperoxide synthase 2 (Ptgs2) in breast cancer tissues Ptgs2-related prognosis for patients with breast cancer. Growth of 4T1 cells was assessed using wound healing assay and MTT assay. The levels of 4-HNE, GPX4, PTGS2 and GSK-3β proteins were detected by Western blot, and the mRNA of Ptgs2 was tested by qPCR. The GSH and MDA were determined by commercial kits. Molecular docking was employed to study interaction between indirubin and GSK-3β. An 4T1 murine breast cancer was adopted to evaluate the in vivo antitumor activity of indirubin.\u0000Results\u0000\u0000Indirubin promoted ferroptosis of 4T1 breast cancer cells with deplete of GSH, increased MDA and 4-HNE level, as well as decreased GPX4 expression. Indirubin suppressed the growth of 4T1 breast tumor in vivo. Mechanism study showed indirubin up regulated Ptgs2 expression by promoting phosphorylation (Ser 9) of GSK-3β.\u0000Conclusions\u0000\u0000Indirubin suppresses 4T1 murine breast cancer in vitro and in vivo by induction of ferroptosis and up-regulation of Ptgs2.","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83551022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.53388/mhm2021b0412001
Hongyun Bao, R. Wen, Xuanya Li, Chen Zhao, Zhineng Chen
{"title":"A brief overview of traditional Chinese medicine prescription powered by artificial intelligence","authors":"Hongyun Bao, R. Wen, Xuanya Li, Chen Zhao, Zhineng Chen","doi":"10.53388/mhm2021b0412001","DOIUrl":"https://doi.org/10.53388/mhm2021b0412001","url":null,"abstract":"","PeriodicalId":23141,"journal":{"name":"TMR Modern Herbal Medicine","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78072234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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