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Bibliometric analysis and visual atlas of mercury-containing Chinese medicine: Hongfen (hydrargyrum oxydatum crudum) 含汞中药:红粉的文献计量学分析及视觉图谱
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023a0206002
Yuk Yin, Yi-fan Cheng, Yi-Qin Chen, M. Ye, Hong-feng Chen
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引用次数: 0
Exploring the mechanism of Huangjing pill in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking 基于网络药理学和分子对接探索黄精丸治疗阿尔茨海默病的作用机制
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023a0209001
Shuanghui Shi, Menglin Wang, Xiaotong Wei, Siddique Ma, Yu-Feng Hu, Jingqiu Zhang, Huinan Wang, Meng-Yu Chen, Qian-Qian Liu, Ying-Zi Wang
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引用次数: 0
Traditional Chinese medicine for the treatment of endometriosis:a comprehensive review of treatment mechanisms 中医药治疗子宫内膜异位症:治疗机制综述
Pub Date : 2023-01-01 DOI: 10.53388/mhm20230010
Chong Feng, Da Zhao, Xiaolong Wang, Man Xiao
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引用次数: 0
Antioxidant activity of six essential oils and its molecules in Ecuadorian Andean medicinal plants 厄瓜多尔安第斯山脉药用植物中六种精油及其分子的抗氧化活性
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023011
P. Noriega, Edison Díaz, Ivana Villegas, K. Pozo, Priscila Guerrero, Pablo Guerra, Christian Larenas
Background : This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes. Methods : The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry. For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods: diphenyl-1-picrylhydrazyl (DPPH), 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and β-Carotene bleaching test. The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method. Results : The scavenging capacity of the radicals was assessed with DPPH and ABTS methods, the best results were found in the oils of M. mollis IC 50 DPPH 2.80 mg/ml and IC 50 ABTS 0.205 mg/mL and in A. glutinosa IC 50 DPPH 12.972 mg/mL and IC 50 ABTS 0.321 mg/mL, the results were compared with a pattern of natural reference in this case, the essential oil of T. vulgaris IC 50 DPPH 0.474 mg/mL and IC 50 ABTS 0.272 mg/mL. The evaluation of the antioxidant activity was determined by the β-carotene bleaching test, the most notable activity results were from M. mollis IC 50 0.119 mg/mL, A. glutinosa IC 50 0.062 mg/mL and B. latifolia IC 50 0,064 mg/mL. DPPH bioautography revealed the active molecules antioxidants in oils for M. mollis were thymol acetate (7.73%) and carvacrol acetate (24.52%), for A. glutinosa was γ-muurolene (2.68%), and for B. latifolia Z-caryophyllene (2.99%), aristolochene (0.11%) and cis-cadin-4-en-7-ol (4.11%). Conclusion : The results of antioxidant activity shown in descending order that the essential oils of: M. mollis , A. glutinosa and B. latifolia , are those with the highest activity using the DPPH and ABTS methods. The β-Carotene bleaching test method confirms the 3 oils as the most active in the following order: A. glutinosa , B. latifolia and M. mollis . An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.
背景:本研究对厄瓜多尔安第斯山脉6种药用植物精油的抗氧化活性及其相关分子进行了研究。方法:采用气相色谱联用质谱法测定精油的化学成分。采用二苯基-1-吡啶肼(DPPH)、2,2′-氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)和β-胡萝卜素漂白法测定精油的抗氧化活性。采用生物autographic HP-TLC-DPPH法对活性较好的精油进行了鉴定。结果:DPPH的清除自由基的能力评估和abt方法,最好的结果被发现的油50 m . mollis IC DPPH 2.80毫克/毫升,IC 50 a abt 0.205毫克/毫升,中需IC 50 DPPH 12.972毫克/毫升,IC 50 abt 0.321毫克/毫升,结果与自然参考的模式相比,在这种情况下,作物的精油IC 50 DPPH 0.474毫克/毫升,IC 50 abt 0.272 mg / ml。通过β-胡萝卜素漂白试验对其抗氧化活性进行评价,结果表明,黄芪ic50 0.119 mg/mL、黄芪ic50 0.062 mg/mL、黄芪ic50 0,064 mg/mL的抗氧化活性最高。DPPH生物自谱分析结果显示,黄芪精油中抗氧化剂活性分子主要为百里酚乙酸酯(7.73%)和香芹酚乙酸酯(24.52%),黄芪精油中抗氧化剂活性分子主要为γ- murolene(2.68%),黄芪精油中抗氧化剂活性分子主要为z -石竹烯(2.99%)、马兜铃烯(0.11%)和顺式cadin-4-烯-7-醇(4.11%)。结论:DPPH法和ABTS法测定的抗氧化活性由高到低依次为黄花草挥发油、黄花草挥发油和荷叶草挥发油。β-胡萝卜素漂白试验法证实,3种精油的活性依次为:粘多糖、荷叶多糖和毛茛。一项抗氧化剂生物自显影研究确定了三种具有良好活性的精油中负责活性的分子。
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引用次数: 0
Exploring the scientific rationality of “Different dosage forms of the same prescription” base on Q-markers of pulvis and pill of Chuanxiong Chatiao Prescription 以川芎茶调方丸为研究对象,探讨“同方不同剂型”的科学合理性
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023012
Ying Liu, Xiao-Fei Zhang, Dongyan Guo, Bing-tao Zhai, Junbo Zou, Yajun Shi
Objective: To predict and analyze the potential Q-markers of Chuanxiong Chatiao Prescription, and the pharmacokinetic properties of pulvis and pills in vivo were studied, which provided a basis for the rational evaluation of the phenomenon of “Different Dosage Forms of the Same Prescription”. Methods and Material: Q-markers analysis of Chuanxiong Chatiao Prescription based on the “Five Principles” (traceability and transmissibility, specificity, effectiveness, prescription compatibility and testability). The content determination method of Q-markers in Chuanxiong Chatiao Prescription was established by UPLC, and the content difference of Q-markers in the two dosage forms ware determined and compared. The Q-markers in rabbit plasma was determined by LC-MS / MS method, and the pharmacokinetic parameters of Q-markers in pulvis and pills were analyzed. Results: A total of 16 potential Q-markers from the “Five Principles” were used, nine components of tetramethylprazine, ferulic acid, glycyrrhizin, glycyrrhizic acid, luteolin, cimicifugoside, senkyunolide Ⅰ, isoimperatorin, nodakenin were identified as Q-markers of Chuanxiong Chatiao Presciption. The content of tetramethylprazine and other components in the pulvis form was found to be significantly higher than that in the pills, while the content of senkyunolide Ⅰ was lower than that in the pills, which may be related to the preparation process of the dosage form and the physicochemical properties of the components. Compared with pulvis, the Tmax and t 1/2 of ferulic acid and other components in pills were significantly prolonged. To a certain extent, it can explain the classical theory of traditional Chinese medicine “Components in pulvis release quickly and take effect in fast-acting manner, while in pills release slowly and take effect in slow-acting”. Meanwhile, the Cmax and AUC0-t of tetramethylprazine and other components in pills were higher than those in pulvis, which showed unexpected pharmacokinetic characteristics, indicating the complexity of compounding and the importance of dosage form design. Conclusions: A method for the determination of Q-markers content was established by UPLC, which provide reference for the quality control of Chuanxiong Chatiao Prescription. In vivo studies have found the pharmacokinetic parameters indicate the absorption and distribution characteristics of pulvis and pills. However, it is also found that the release behavior of different components not only affected by the dosage form but also closely related to their own physical and chemical properties
目的:预测分析川芎茶调方的潜在q -标志物,研究其丸体和丸体的体内药动学特性,为合理评价“同方不同剂型”现象提供依据。方法和材料:依据“五项原则”(可追溯性和传导性、特异性、有效性、处方配伍性和可检测性)对川芎茶调方进行q标记分析。采用超高效液相色谱法建立川芎茶调方中q标记物的含量测定方法,并对两剂型中q标记物的含量差异进行了测定和比较。采用LC-MS / MS法测定兔血浆中q -标志物的含量,并分析其在pulvis和药丸中的药动学参数。结果:利用“五大原则”中的16个潜在q标记物,鉴定出川芎茶调方的q标记物为川芎茶调方的四甲基吡嗪、阿魏酸、甘草酸、甘草酸、木犀草素、山茱萸苷、仙球内酯Ⅰ、异欧前胡素、野木犀草素9种成分。发现丸状中四甲基吡嗪等成分含量显著高于丸状,而仙球内酯Ⅰ含量低于丸状,这可能与剂型的制备工艺及成分的理化性质有关。丸中阿魏酸等成分的Tmax和t1 /2均明显延长。在一定程度上可以解释“骨部成分释放快,见效快,丸中成分释放慢,见效慢”的经典中医理论。同时,丸中四甲基吡嗪等成分的Cmax和AUC0-t均高于丸中四甲基吡嗪等成分的Cmax和AUC0-t,表现出意想不到的药代动力学特征,说明复方的复杂性和剂型设计的重要性。结论:建立了超高效液相色谱法测定川芎茶调方中q标记物含量的方法,可为川芎茶调方的质量控制提供参考。体内研究发现,药代动力学参数反映了pulvis和药丸的吸收和分布特征。然而,也发现不同成分的释放行为不仅受剂型的影响,而且与其本身的理化性质密切相关
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引用次数: 0
Differential distribution of authenticity results of different varieties of refined honey based on stable isotope ratio method 基于稳定同位素比值法的不同品种精制蜂蜜真伪结果差异分布
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023015
Siddique Ma, Yu-Feng Hu, Xiao-Tong Wei, Menglin Wang, Shuang-Hui Shi, Ming-Rui Jiang, Hui-Nan Wang, Jing-Qiu Zhang, Ying-Zi Wang, Dao-Bing Wang
Objective Using the stable isotope ratio method for the authenticity identification and variety identification of refined honey. Methods In this paper, a total of 17 samples of different varieties of refined honey were used to obtain refined honey proteins by precipitation with sodium tungstate solution and sulphuric acid solution. The isotope mass spectrometer was used to simultaneously detect the δ 13 C values of refined honey proteins and refined honey as well as the δ 18 O and δ 2 H values of refined honey, processed of the results obtained, analysed the authenticity of the samples and conduct a variety identification study. Results Tested of the resulting honey samples, the results showed that four batches of refined honey did not up to standard, two batches of C-4 vegetable syrup were detected as adulterated, and two batches of protein were not detected. The δ 18 O and δ 2 H values of refined honey were also found to be effective in distinguishing the varietal origin of refined honey to a certain extent. Conclusions The stable isotope ratio method is useful in the authenticity identification of refined honey, and provides new ideas to further promote the authenticity of refined honey and variety identification research.
目的采用稳定同位素比值法对精制蜂蜜进行真伪鉴定和品种鉴定。方法采用钨酸钠溶液和硫酸溶液沉淀法制备17种不同品种的精制蜂蜜蛋白。利用同位素质谱仪同时检测了精制蜂蜜蛋白和精制蜂蜜的δ 13c值以及精制蜂蜜的δ 18o和δ 2h值,并对所得结果进行了处理,分析了样品的真实性,进行了品种鉴定研究。结果对所得蜂蜜样品进行检测,发现4批次精制蜂蜜不合格,2批次C-4植物糖浆掺假,2批次蛋白质未检出。精制蜂蜜的δ 18o和δ 2h值在一定程度上也能有效区分精制蜂蜜的品种来源。结论稳定同位素比值法可用于精制蜂蜜的真实性鉴定,为进一步推进精制蜂蜜的真实性及品种鉴定研究提供了新的思路。
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引用次数: 0
Therapeutic effects of Astragali Radix on diabetic foot: a clinical randomized controlled trial 黄芪治疗糖尿病足的临床随机对照试验
Pub Date : 2023-01-01 DOI: 10.53388/mhm2023013
Zhengang Du, Honggang Ni, Qian Guo, Li-dan Zhang
Objective: This study was conducted to evaluate the efficacy of Astragalus on diabetic foot, as well as the effects on the levels of serum VEGF, bFGF, MMP-2, and inflammatory factors in patients, and to provide a scientific basis for the treatment of diabetic foot with the traditional Chinese medicine Astragalus . Methods: By taking 100 cases of diabetic foot patients who were admitted to the metabolic internal medicine division of the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine and met the criteria of natriuresis during April 2021–April 2023 as the study subjects, and according to the random number method, all patients were divided into the control group and the observation group, with 50 cases in each group. In the control group, only basic treatment was carried out, while in the observation group, Astragalus injection was added based on the control group. After 8 weeks of treatment, the clinical efficacy, serum VEGF, bFGF, MMP-2, and inflammatory factor levels of the patients in the two groups were compared, respectively. Results: The total clinical efficiency of patients in the observation group was significantly better than that in the control group ( χ 2 =5.01, P < 0.05). The inflammatory factor indexes decreased substantially in both groups. However, the decrease in the observation group was significantly higher than in the control group ( P < 0.05). Compared with the control group, serum VEGF and bFGF were considerably higher in the observation group, while MMP-2 was significantly lower ( P < 0.05). Conclusion: Astragali Radix is clinically effective in the diabetic foot, which can induce vascular endothelial repair and reduce the level of inflammatory factors, to improve the inflammatory state of patients and promote the restoration of ulcerated wound tissue, which is worth promoting in clinical practice.
目的:本研究旨在评价黄芪对糖尿病足的疗效,以及对患者血清VEGF、bFGF、MMP-2及炎症因子水平的影响,为中药黄芪治疗糖尿病足提供科学依据。方法:以贵州中医药大学附属第二医院代谢内科2021年4月至2023年4月收治的符合尿钠标准的糖尿病足患者100例为研究对象,按随机数字法将患者分为对照组和观察组,每组各50例。对照组仅进行基础治疗,观察组在对照组的基础上加用黄芪注射液。治疗8周后,比较两组患者的临床疗效、血清VEGF、bFGF、MMP-2及炎症因子水平。结果:观察组患者临床总有效率显著优于对照组(χ 2 =5.01, P < 0.05)。两组患者炎症因子指数均明显下降。但观察组下降幅度显著高于对照组(P < 0.05)。与对照组比较,观察组患者血清VEGF、bFGF显著升高,MMP-2显著降低(P < 0.05)。结论:黄芪对糖尿病足具有临床疗效,可诱导血管内皮修复,降低炎症因子水平,改善患者炎症状态,促进溃疡创面组织修复,值得临床推广。
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引用次数: 0
Assessment of perception of healthcare professionals regarding the use of herbal medicines in Islamabad, Pakistan: A cross-sectional study 评估医疗保健专业人员对巴基斯坦伊斯兰堡使用草药的看法:一项横断面研究
Pub Date : 2022-01-01 DOI: 10.53388/mhm2022qi0329001
Humna Baig
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引用次数: 1
The anti-pulmonary fibrosis of Salvia miltiorrhiza Bunge: A systematic review 丹参抗肺纤维化的系统评价
Pub Date : 2022-01-01 DOI: 10.53388/mhm2022b0418001
Jiawei Li, B. Jia, Jiachen He, Bin Xing, Ying Zhang
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引用次数: 0
Clinical observation and experimental study on Kangfuxin fluid in treating indwelling needle-related phlebitis 康复心液治疗留置针相关性静脉炎的临床观察及实验研究
Pub Date : 2022-01-01 DOI: 10.53388/mhm2022a0917001
Lifang Zou, Ying Dong, Ni Xu, Jing-yu Quan, Ling-Na Dai, Jun-Fang Liu
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引用次数: 0
期刊
TMR Modern Herbal Medicine
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