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Effect of wheat middling incorporation into wet corn distillers grains with solubles on apparent diet digestibility and ruminal fermentation characteristics in growing and finishing diets 在湿玉米蒸馏谷物(含溶解物)中添加小麦中间产物对生长期和育成期日粮表观日粮消化率和瘤胃发酵特性的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-05-14 DOI: 10.1093/tas/txae083
Z. Duncan, Z. DeBord, M. Pflughoeft, W. R. Hollenbeck, E. C. Titgemeyer, K. C. Olson, D. A. Blasi
Two separate cross-over experiments were conducted to evaluate effects of incorporating wheat middlings into wet corn distillers grains with solubles (WDGS) on apparent diet digestibility and ruminal fermentation characteristics in growing and finishing diets. In Exp. 1, 4 ruminally-cannulated heifers (313 ± 42.9 kg) were limit fed a high-energy growing diet that included WDGS (CON) or WDGS + wheat middlings (CON+WM) at 40% of diet dry matter (DM). The diet also contained (DM basis) 39.5% dry-rolled corn, 7.5% supplement, and 13% warm-season grass hay. In Exp. 2, 4 ruminally-cannulated Holstein steers (321 ± 17.4 kg) were fed a finishing diet that included WDGS (CON) or WDGS + wheat middlings (CON+WM) at 30% of diet DM. The diet also contained (DM basis) 60.3% dry-rolled corn, 2.7% supplement, and 7.0% warm-season grass hay. Experiments consisted of two 15-d periods that were conducted concurrently. Each period included 10 d of diet adaptation, 4 d of fecal collection, and 1 d of ruminal fluid collection. Fecal samples were collected on d 11 to d 14 of each period and composite samples were analyzed for chromium to estimate apparent diet digestibility. On d 15, ruminal fluid samples were collected prior to feeding and again at 2, 4, 6, 8, 12, 18, and 24-h post-feeding. In Exp. 1, DM, organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) intake did not differ (P ≥ 0.11) between diets; however, starch intake was greater (P = 0.03) for heifers fed CON+WM compared with CON. Apparent DM, OM, NDF, and starch digestibilities were similar between diets (P ≥ 0.13), but feeding CON+WM tended to lead to lesser (P = 0.06) apparent ADF digestibility. Ruminal pH and total volatile fatty acid concentrations did not differ between diets (P ≥ 0.16); however, ruminal ammonia concentrations tended to be less (P = 0.09) for CON+WM compared with CON. In Exp. 2, DM intake did not differ (P = 0.65) between diets. Apparent DM digestibility was greater (P = 0.01) for CON+WM compared with CON but the difference was small. Intake and apparent digestibility of OM, NDF, ADF, and starch did not differ (P ≥ 0.25) between diets. Ruminal ammonia concentrations were lesser (P = 0.03) while ruminal pH was greater (P = 0.02) for CON+WM compared with CON. Overall, incorporation of wheat middlings into WDGS had minimal impacts on feed intake, apparent diet digestibility, and ruminal fermentation characteristics when fed to growing and finishing cattle.
我们分别进行了两项交叉实验,以评估在湿玉米蒸馏谷物加溶质(WDGS)中添加小麦胚芽米对生长日粮和育成日粮中表观日粮消化率和瘤胃发酵特性的影响。在实验 1 中,4 头反刍小母牛(313 ± 42.9 千克)被限制饲喂高能量生长日粮,其中包括占日粮干物质(DM)40% 的 WDGS(CON)或 WDGS + 小麦胚芽(CON+WM)。日粮中还含有(DM 基础)39.5% 的干轧玉米、7.5% 的补充剂和 13% 的暖季草干草。在实验 2 中,给 4 头反刍的荷斯坦小公牛(321 ± 17.4 千克)饲喂了包括 WDGS(CON)或 WDGS + 小麦粉(CON+WM)的育成日粮,其中 WDGS 占日粮 DM 的 30%。日粮中还含有(DM 基础)60.3% 的干轧玉米、2.7% 的补充剂和 7.0% 的暖季型干草。实验分为两个 15 天的阶段,同时进行。每个阶段包括 10 天的日粮适应期、4 天的粪便收集期和 1 天的瘤胃液收集期。在每个阶段的第 11 天至第 14 天收集粪便样本,并对复合样本进行铬分析,以估算日粮的表观消化率。第 15 天,在饲喂前收集瘤胃液样品,并在饲喂后 2、4、6、8、12、18 和 24 小时再次收集瘤胃液样品。在实验 1 中,不同日粮的 DM、有机物(OM)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)摄入量没有差异(P ≥ 0.11);但是,与 CON 相比,饲喂 CON+WM 的小母牛的淀粉摄入量更大(P = 0.03)。不同日粮的表观DM、OM、NDF和淀粉消化率相似(P ≥ 0.13),但饲喂CON+WM往往导致表观ADF消化率较低(P = 0.06)。不同日粮的瘤胃 pH 值和总挥发性脂肪酸浓度没有差异(P ≥ 0.16);但是,与 CON 相比,CON+WM 的瘤胃氨浓度往往较低(P = 0.09)。在实验 2 中,不同日粮的 DM 摄入量没有差异(P = 0.65)。与 CON 相比,CON+WM 的 DM 表观消化率更高(P = 0.01),但差异很小。不同日粮的 OM、NDF、ADF 和淀粉摄入量和表观消化率没有差异(P ≥ 0.25)。与 CON 相比,CON+WM 的瘤胃氨浓度更低(P = 0.03),而瘤胃 pH 值更高(P = 0.02)。总之,在 WDGS 中添加小麦胚芽对生长牛和育成牛的采食量、日粮表观消化率和瘤胃发酵特性的影响很小。
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引用次数: 0
Models to predict nitrogen excretion from beef cattle fed a wide range of diets compiled from South America. 从南美洲汇编的各种日粮中预测肉牛氮排泄量的模型。
IF 1.3 Q2 Veterinary Pub Date : 2024-05-09 eCollection Date: 2024-01-01 DOI: 10.1093/tas/txae072
Vinícius C Souza, Guilhermo F S Congio, João P P Rodrigues, Sebastião C Valadares Filho, Flávia A S Silva, Luciana N Rennó, Ricardo A Reis, Abmael S Cardoso, Paulo H M Rodrigues, Telma T Berchielli, Juliana D Messana, Cecilia Cajarville, Yury T Granja-Salcedo, Ana L C C Borges, Gilberto V Kozloski, Jaime R Rosero-Noguera, Horacio Gonda, Alexander N Hristov, Ermias Kebreab

The objective of this meta-analysis was to develop and evaluate models for predicting nitrogen (N) excretion in feces, urine, and manure in beef cattle in South America. The study incorporated a total of 1,116 individual observations of N excretion in feces and 939 individual observations of N excretion in feces and in urine (g/d), representing a diverse range of diets, animal genotypes, and management conditions in South America. The dataset also included data on dry matter intake (DMI; kg/d) and nitrogen intake (NI; g/d), concentrations of dietary components, as well as average daily gain (ADG; g/d) and average body weight (BW; kg). Models were derived using linear mixed-effects regression with a random intercept for the study. Fecal N excretion was positively associated with DMI, NI, nonfibrous carbohydrates, average BW, and ADG and negatively associated with EE and CP concentration in the diet. The univariate model predicting fecal N excretion based on DMI (model 1) performed slightly better than the univariate model, which used NI as a predictor variable (model 2) with a root mean square error (RMSE) of 38.0 vs. 39.2%, the RMSE-observations SD ratio (RSR) of 0.81 vs. 0.84, and concordance correlation coefficient (CCC) of 0.53 vs. 0.50, respectively. Models predicting urinary N excretion were less accurate than those derived to predict fecal N excretion, with an average RMSE of 43.7% vs. 37.0%, respectively. Urinary and manure N excretion were positively associated with DMI, NI, CP, average BW, and ADG and negatively associated with neutral detergent fiber concentration in the diet. As opposed to fecal N excretion, the univariate model predicting urinary N excretion using NI (model 10) performed slightly better than the univariate model using DMI (model 9) as predictor variable with an RMSE of 36.0% vs. 39.7%, RSR 0.85 vs. 0.93, and CCC of 0.43 vs. 0.29, respectively. The models developed in this study are applicable for predicting N excretion in beef cattle across a broad spectrum of dietary compositions and animal genotypes in South America. The univariate model using DMI as a predictor is recommended for fecal N prediction, while the univariate model using NI is recommended for predicting urinary and manure N excretion because the use of more complex models resulted in little to no benefits. However, it may be more useful to consider more complex models that incorporate nutrient intakes and diet composition for decision-making when N excretion is a factor to be considered. Three extant equations evaluated in this study have the potential to be used in tropical conditions typical of South America to predict fecal N excretion with good precision and accuracy. However, none of the extant equations are recommended for predicting urine or manure N excretion because of their high RMSE, and low precision and accuracy.

这项荟萃分析的目的是开发和评估用于预测南美洲肉牛粪便、尿液和粪便中氮(N)排泄量的模型。该研究共纳入了 1,116 份粪便中氮排泄量的个体观测数据和 939 份粪便和尿液中氮排泄量(克/天)的个体观测数据,这些数据代表了南美洲不同的日粮、动物基因型和管理条件。数据集还包括干物质摄入量(DMI;kg/d)和氮摄入量(NI;g/d)、日粮成分浓度以及平均日增重(ADG;g/d)和平均体重(BW;kg)的数据。采用线性混合效应回归法得出模型,并为研究设置了随机截距。粪N排泄量与DMI、NI、非纤维碳水化合物、平均体重和ADG呈正相关,与日粮中EE和CP浓度呈负相关。以 DMI 为基础预测粪氮排泄量的单变量模型(模型 1)的表现略好于以 NI 为预测变量的单变量模型(模型 2),其均方根误差(RMSE)分别为 38.0% 和 39.2%,均方根误差-观测值标度比(RMSE-Observations SD ratio,RSR)分别为 0.81 和 0.84,一致性相关系数(conordance correlation coefficient,CCC)分别为 0.53 和 0.50。预测尿氮排泄量的模型不如预测粪氮排泄量的模型准确,其平均有效误差率分别为 43.7% 和 37.0%。尿氮和粪氮排泄量与DMI、NI、CP、平均体重和ADG呈正相关,而与日粮中的中性洗涤纤维浓度呈负相关。与粪氮排泄量相比,使用 NI 预测尿氮排泄量的单变量模型(模型 10)比使用 DMI 预测尿氮排泄量的单变量模型(模型 9)表现稍好,RMSE 分别为 36.0% 对 39.7%,RSR 分别为 0.85 对 0.93,CCC 分别为 0.43 对 0.29。本研究建立的模型适用于预测南美洲各种日粮组成和动物基因型的肉牛氮排泄量。建议使用以 DMI 为预测因子的单变量模型预测粪氮,而使用 NI 的单变量模型预测尿氮和粪氮的排泄,因为使用更复杂的模型几乎没有任何益处。不过,当氮排泄是一个需要考虑的因素时,考虑使用包含营养摄入量和日粮组成的更复杂模型进行决策可能会更有用。本研究中评估的三个现有方程有可能用于南美洲典型的热带条件,以良好的精度和准确性预测粪氮排泄。但是,由于这些方程的均方根误差较大,精度和准确度较低,因此不建议将它们用于预测尿液或粪便的氮排泄量。
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引用次数: 0
Effect of extended days on feed on carcass gain, efficiency, and quality of individually fed beef steers 延长饲喂天数对单独饲喂肉牛的胴体增重、效率和质量的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-05-08 DOI: 10.1093/tas/txae081
J. L. Sperber, R. Bondurant, G. Erickson, K. Bruns, R. Funston, J. C. MacDonald
Crossbred steers (n = 114, initial BW = 334 kg; SEM = 5 kg) were serially harvested to evaluate the change in carcass composition by feeding cattle 21 or 42 d longer than the 2014 industry average subjective measure of finish, 1.27 cm of 12th rib fat thickness. Carcass ultrasound measurements were collected on 76 steers at 1, 78, and 134 days on feed (DOF) to project appropriate harvest date. Steers were sorted into three harvest groups, and serially harvested at 142, 163, or 185 DOF, with the first harvest date selected based on an estimated 12th rib fat thickness of 1.27 cm via ultrasound measurement. Steers were fed using an individual animal feeding system, to determine individual performance metrics. Steer DMI did not differ (P ≥ 0.31) between harvest groups, while carcass-adjusted ADG and G:F decreased linearly (P ≤ 0.04) as DOF increased. Carcass weight increased linearly (P < 0.01) as DOF increased from 142 to 185 DOF, with steers gaining an additional 36 kg of HCW when fed an additional 42 DOF. Carcass LM area quadratically increased (P = 0.04) to 163 DOF and remained constant to 185 DOF. Marbling score was not different (P = 0.14) between harvest groups, however, the opportunity to grade USDA Premium Choice was improved for steers fed to 185 DOF. Calculated YG and 12th rib fat thickness increased linearly (P < 0.01) as DOF increased, with distributions across YG 1 through 5 differing between harvest groups (P < 0.01), and 185-d carcasses having the greatest frequency of YG 4 carcasses. As cattle are fed for additional DOF, live ADG and G:F decline, while HCW and LM area increase.
对杂交母牛(n = 114 头,初始体重 = 334 千克;SEM = 5 千克)进行连续采收,以评估胴体成分的变化,饲喂时间比 2014 年行业平均主观完成度(第 12 肋脂肪厚度 1.27 厘米)长 21 或 42 天。在饲养 1 天、78 天和 134 天 (DOF) 时,对 76 头母牛进行了胴体超声波测量,以预测适当的收获日期。阉牛被分为三个收获组,分别在 142、163 或 185 DOF 时收获,第一个收获日期根据超声波测量估计的第 12 肋骨脂肪厚度 1.27 厘米确定。使用个体动物饲喂系统饲喂母牛,以确定个体性能指标。收获组之间的阉牛DMI没有差异(P≥ 0.31),而胴体调整后的ADG和G:F随着DOF的增加呈线性下降(P≤ 0.04)。胴体重量随着DOF从142增加到185呈线性增加(P < 0.01),当饲喂额外的42 DOF时,阉牛的HCW增加了36公斤。胴体 LM 面积在 163 DOF 时呈二次增长(P = 0.04),在 185 DOF 时保持不变。收获组之间的大理石纹得分没有差异(P = 0.14),但饲喂到 185 DOF 的阉牛的 USDA 特级选择分级机会有所提高。计算的 YG 和第 12 肋骨脂肪厚度随着 DOF 的增加而线性增加(P < 0.01),不同收获组之间 YG 1 到 5 的分布有所不同(P < 0.01),185-d 胴体中 YG 4 的频率最高。随着牛的DOF增加,活体ADG和G:F下降,而HCW和LM面积增加。
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引用次数: 0
Effect of traditional roughage-based or limit-fed, high-energy diets on growth performance and digestion in newly received growing cattle and subsequent implications on feedlot growth performance and carcass characteristics 传统粗饲料日粮或限饲高能量日粮对新接收生长牛生长性能和消化能力的影响,以及随后对饲养场生长性能和胴体特征的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-05-08 DOI: 10.1093/tas/txae082
M. A. Scilacci, E. C. Titgemeyer, Z. Duncan, T. Spore, S. P. Montgomery, Travis G O'Quinn, A. J. Tarpoff, W. R. Hollenbeck, D. A. Blasi
The objective was to determine effects of ad libitum-fed roughage-based diets or limit-fed high-energy diets on growth performance, behavior, health, and digestion in newly received growing cattle and subsequent implications on feedlot growth performance and carcass characteristics. In Exp. 1, 409 crossbred heifers (initial body weight [BW] = 279 ± 24 kg) in 32 pens were used in a randomized block design. Heifers were fed one of two dietary treatments: a total mixed ration with 0.99 Mcal net energy for gain (NEg)/kg dry matter (DM) fed ad libitum (0.99AL) or 1.32 Mcal NEg/kg DM limit-fed at 85% of intake of heifers fed 0.99AL (1.32LF85%). Both diets contained 40% of DM as a branded wet corn gluten feed. In Exp. 2, 370 crossbred heifers (initial BW = 225 ± 20 kg) were used in a randomized block design and were fed a diet formulated to contain 0.99 Mcal of NEg/kg DM for ad libitum intake or a diet formulated to contain 1.32 Mcal of NEg/kg DM and fed at 2.2% of BW daily (DM basis; 1.32LF2.2). For Exp. 1 and 2, treatment integrity was maintained through the finishing phase where cattle were fed a common diet. Cattle were sorted by BW into heavy and light groups prior to finishing, with light cattle fed longer than heavy cattle to reach similar harvest BW. In Exp. 3, 8 ruminally-cannulated heifers (average BW = 305 ± 23 kg) were used in a 2-period cross-over design and fed treatments from Exp. 1 to assess digestibility and ruminal fermentation characteristics. Gain:feed was 47% and 35% greater (P < 0.01) in Exp. 1 and 2, respectively, for limit-fed heifers compared with 0.99AL heifers. Rumination time was greater (P < 0.01) for 0.99AL compared with limit-fed treatments in Exp. 1 and 2. Activity was greater (P < 0.01) for 1.32LF2.2 than for 0.99AL in Exp. 2. In Exp. 1, more (P = 0.03) carcasses from light sort heifers than carcasses from heavy sort heifers had livers with large, active abscesses. In Exp. 2, finishing-phase morbidity was greater (P < 0.01) for 1.32LF2.2 than for 0.99AL. Light-sort groups had fewer (P < 0.01) edible livers than heavy-sort groups, suggesting that greater number of days on feed may increases the risk of liver abscess prevalence and condemnation. In Exp. 3, apparent total-tract DM and organic matter digestibilities were greater (P < 0.01) for 1.32LF85% than for 0.99AL. Overall, dietary treatments during the growing phase had little carryover effect on feedlot growth performance, carcass characteristics, or liver abscesses prevalence at harvest.
目的是确定自由采食粗饲料日粮或限制采食高能量日粮对新接收生长牛的生长性能、行为、健康和消化的影响,以及随后对饲养场生长性能和胴体特征的影响。在实验 1 中,采用随机区组设计,将 409 头杂交小母牛(初始体重 [BW] = 279 ± 24 千克)分养在 32 个牛栏中。小母牛饲喂两种日粮中的一种:0.99Mcal 净增重能(NEg)/kg 干物质(DM)的全混日粮(0.99AL)或 1.32Mcal NEg/kg DM 限饲日粮,限饲量为饲喂 0.99AL 的小母牛摄入量的 85% (1.32LF85%)。两种日粮都含有 40% 的品牌湿玉米麸质饲料 DM。在实验 2 中,采用随机区组设计饲养了 370 头杂交小母牛(初始体重 = 225 ± 20 千克),饲喂的日粮配方为每千克 DM 含 0.99 毫克 NEal,供自由采食;或饲喂的日粮配方为每千克 DM 含 1.32 毫克 NEal,日采食量为体重的 2.2%(DM 基数;1.32LF2.2)。在试验 1 和试验 2 中,处理的完整性一直保持到牛的育成期,在育成期牛饲喂的是普通日粮。牛在采食前按体重分为重型组和轻型组,轻型牛的采食时间比重型牛长,以达到相似的采食体重。在实验 3 中,8 头反刍小母牛(平均体重 = 305 ± 23 千克)采用两期交叉设计,饲喂实验 1 中的处理,以评估消化率和瘤胃发酵特性。与 0.99AL 母牛相比,试验 1 和试验 2 中限饲母牛的饲料增重比分别高出 47% 和 35% (P < 0.01)。在实验 1 和 2 中,与限饲处理相比,0.99AL 处理的反刍时间更长(P < 0.01)。在实验 2 中,1.32LF2.2 比 0.99AL 的活动量更大(P < 0.01)。 在实验 1 中,轻度分选母牛的胴体比重度分选母牛的胴体上有更多的(P = 0.03)肝脏有大的活动性脓肿。在实验 2 中,1.32LF2.2 比 0.99AL 的育成期发病率更高(P < 0.01)。与重度分选组相比,轻度分选组的可食用肝脏数量较少(P < 0.01),这表明饲喂天数越多,肝脓肿流行和被判刑的风险就越大。在实验 3 中,1.32LF85% 的表观总消化率(DM)和有机物消化率(P < 0.01)高于 0.99AL 的表观总消化率(DM)和有机物消化率(P < 0.01)。总之,生长阶段的日粮处理对饲养场的生长性能、胴体特征或收获时的肝脓肿发病率几乎没有影响。
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引用次数: 0
Evaluation of Elnady preserved tissues as a teaching aid for undergraduate animal science courses 埃尔纳迪保存组织作为本科动物科学课程教学辅助工具的评估
IF 1.3 Q2 Veterinary Pub Date : 2024-05-07 DOI: 10.1093/tas/txae077
J. A. Daniel, I. Kukor
The use of tissue specimens for undergraduate instruction is a very valuable tool. However, fresh tissue specimens are not always available and many common preservation techniques can result in discoloration, offensive odors, and/or dangerous chemical residues. The Elnady Technique was developed as a means to produce tissue specimens that “are realistic, durable, have no offensive odor, and are dry, soft and flexible” (Elnady, F.A. 2016 The Elnady Technique: An innovative, new method for tissue preservation. Altex. 33:237-242. doi:10.14573/altex.1511091). Briefly for soft tissue, specimens were preserved by fixing in formalin. The tissue specimen was then dehydrated with a series of acetone baths. Once the tissue was fully dehydrated, the specimen was impregnated in glycerin. Excess glycerin was then removed by draining followed by immersion in cornstarch. Cornstarch residue was removed with a soft brush, and the specimen was stored in a plastic bag. Multiple specimens (including female reproductive tract of the cat, goat, horse, and sow; digestive tract of cat, chicken, and dog; one day old lamb stomach; goat rumen, reticulum, omasum, and abomasum; and sheep heart and kidney) have been preserved and used in various animal science course laboratories (126 laboratory sections and 1696 students at Berry College). Some of the specimens have been in use for seven years and are still in usable condition. Anonymously surveyed Berry College Animal Science Faculty strongly agreed or agreed Elnady preserved tissues are a useful teaching aid (n = 5). The Elnady Technique has proven to be a useful means of preserving tissue samples used in undergraduate animal science courses.
在本科生教学中使用组织标本是一种非常有价值的工具。然而,新鲜的组织标本并不总能获得,而且许多常见的保存技术会导致褪色、异味和/或危险的化学残留物。Elnady 技术的开发是为了制作出 "逼真、耐用、无异味、干燥、柔软、有弹性 "的组织标本(Elnady, F.A. 2016 Elnady 技术:一种创新的组织保存新方法。Altex.33:237-242. doi:10.14573/altex.1511091).简而言之,对于软组织,标本在福尔马林中固定保存。然后用一系列丙酮浴使组织样本脱水。组织完全脱水后,将标本浸渍在甘油中。然后通过排水去除多余的甘油,再浸泡在玉米淀粉中。用软毛刷清除玉米淀粉残留物,然后将标本保存在塑料袋中。多个标本(包括猫、山羊、马和母猪的雌性生殖道;猫、鸡和狗的消化道;一天大的羔羊胃;山羊瘤胃、网胃、乳头和腹腔;以及绵羊心脏和肾脏)已被保存并用于各种动物科学课程实验室(贝里学院有 126 个实验节和 1696 名学生)。其中一些标本已经使用了 7 年,仍处于可用状态。接受匿名调查的贝瑞学院动物科学系教师非常同意或同意艾尔纳迪保存的组织是一种有用的教学辅助工具(n = 5)。事实证明,Elnady 技术是保存本科动物科学课程所用组织样本的有效方法。
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引用次数: 0
Lupine-induced Crooked Calf Syndrome (CCS): Mitigation through intermittent grazing management of cattle 羽扇豆诱发的小牛弯曲综合症(CCS):通过对牛群进行间歇性放牧管理来缓解症状
IF 1.3 Q2 Veterinary Pub Date : 2024-05-07 DOI: 10.1093/tas/txae079
K. Welch, Stephen T. Lee, K. Panter, Bryan S Stegelmeier, C. Stonecipher, Daniel Cook
Lupines are responsible for a condition in cattle referred to as “crooked calf syndrome” (CCS) that occurs when pregnant cattle graze teratogenic lupines. A proposed management strategy to limit these types of birth defects includes utilizing an intermittent grazing schedule to allow short durations of grazing lupine-infested areas interrupted by movement to a lupine-free pasture. The objective of this study was to determine if an intermittent schedule of ten continuous days on lupine treatment followed by five days off treatment would be sufficient to decrease, or prevent, the incidence of lupine-induced malformations. Continuous dosing of the teratogenic lupine (Lupinus leucophyllus) to pregnant cows for 30 days during the most susceptible stage of pregnancy (gestation days 40-70) resulted in severe skeletal birth defects in their calves. However, intermittent dosing of the teratogenic lupine demonstrated that interrupted intake of lupine reduced the severity, or eliminated, permanent skeletal malformations in calves born to cows dosed lupine. Toxicokinetic and ultrasound data demonstrated a clear inverse correlation between serum anagyrine (the primary teratogenic alkaloid in some lupines) concentrations in the dam and fetal movement. In the intermittent group, fetal movement quickly returned to normal after lupine feeding stopped and remained normal until lupine treatment resumed. Therefore, interrupting lupine intake for at least five days through an intermittent grazing program could reduce the severity of the CCS. Furthermore, this method would allow ranchers to move cattle back into lupine pastures after a brief interruption, which would allow for more efficient utilization of forage resources.
羽扇豆是造成牛 "牛犊歪斜综合症"(CCS)的原因,当怀孕的牛放牧致畸羽扇豆时,就会出现这种情况。为限制这类先天缺陷而提出的管理策略包括利用间歇放牧计划,允许在羽扇豆肆虐的地区进行短时间放牧,然后转移到没有羽扇豆的牧场。本研究的目的是确定连续十天服用羽扇豆,然后休息五天的间歇性计划是否足以减少或防止羽扇豆诱发畸形的发生。在最易受影响的妊娠阶段(妊娠 40-70 天),连续 30 天给妊娠母牛服用致畸羽扇豆(Lupinus leucophyllus),会导致犊牛出现严重的骨骼先天缺陷。然而,间歇性服用致畸羽扇豆表明,间歇性摄入羽扇豆可减轻或消除服用羽扇豆的母牛所产犊牛的永久性骨骼畸形。毒物动力学和超声波数据表明,母体血清中的安乃近(某些羽扇豆中的主要致畸生物碱)浓度与胎动之间存在明显的反相关关系。在间歇组中,羽扇豆喂食停止后,胎动很快恢复正常,并在羽扇豆治疗恢复前保持正常。因此,通过间歇性放牧计划中断羽扇豆摄入至少五天,可减轻CCS的严重程度。此外,这种方法还能让牧场主在短暂中断后将牛群赶回羽扇豆牧场,从而更有效地利用牧草资源。
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引用次数: 0
Effects of feeding α-amylase-expressed corn silage and grain on performance, enteric methane production, and carcass characteristics in beef steers 饲喂α-淀粉酶表达的玉米青贮和谷物对肉牛生产性能、肠道甲烷产量和胴体特征的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-05-07 DOI: 10.1093/tas/txae080
L. Rebelo, Kirsten L Clark, A. Relling, Chanhee Lee
Abstract An experiment was conducted to evaluate the effects of feeding Enogen feed corn (EFC) silage or EFC grain with different grain processing (dry-rolled corn vs. whole-shelled corn) in feedlot cattle diets. Total 68 Angus cross-bred steers were blocked by body weight and the treatments (diets) were randomly assigned to steers in each block: a basal diet with isoline corn silage and isoline dry-rolled corn grain (IIR); the basal diet with EFC silage and isoline dry-rolled corn grain (EIR); the basal diet with EFC silage and EFC dry-rolled grain (EER); and the basal diet with EFC silage and EFC whole-shelled grain (EEW). Isoline refers to the isogenic counterpart of Enogen corn silage or grain. Steers received the assigned treatment over 32 wk of the entire experiment (backgrounding and finishing) until harvested. Part of the steers (eight blocks) in each treatment were used to measure CH4 production (g/d) using the GreenFeed and CH4 production per unit of DMI. All data were analyzed using a mixed procedure of SAS in a randomized complete block design, considering diet as a fixed effect and block as a random effect. Steers fed the EIR diet increased (P = 0.03) DMI compared to IIR during the backgrounding phase. However, feeding EFC silage or grain did not affect body weight, average daily gain, and feed efficiency during backgrounding and finishing phases. Feeding EEW decreased (P ≤ 0.05) body weight, average daily gain, feed efficiency, and tended to decrease (P = 0.06) hot carcass weight compared to EER during the finishing phase. Methane production per unit of DMI decreased (P = 0.02) for steers fed EIR compared with steers fed IIR only during the backgrounding phase. Feeding EFC grain had no effect on CH4 production (g/d) in both phases. In conclusion, feeding EFC silage or grain did not improve the performance of beef steers during the backgrounding and finishing phases in the current experiment condition. Methane production per unit of DMI was reduced for steers fed EFC silage compared with isoline corn silage only during the backgrounding phase.
摘要 进行了一项实验,以评估在饲养场牛日粮中饲喂伊诺金饲料玉米(EFC)青贮饲料或不同谷物加工(干轧玉米与全壳玉米)的 EFC 谷物的效果。共有 68 头安格斯杂交小公牛按体重被分块饲养,每块中的小公牛随机分配到不同的处理(日粮):基础日粮中添加异基因玉米青贮和异基因干轧制玉米粒(IIR);基础日粮中添加 EFC 青贮和异基因干轧制玉米粒(EIR);基础日粮中添加 EFC 青贮和 EFC 干轧制玉米粒(EER);基础日粮中添加 EFC 青贮和 EFC 全壳玉米粒(EEW)。Isoline 指的是与 Enogen 玉米青贮或谷物同源的对应物。在整个实验的 32 周内(背景饲养和育成期),母牛接受指定的处理,直至收获。使用 GreenFeed 和单位 DMI 的 CH4 产量(克/天)测量每个处理中的部分母牛(8 块)。所有数据均采用随机完全区组设计中的 SAS 混合程序进行分析,将日粮作为固定效应,区组作为随机效应。在背景饲养阶段,饲喂 EIR 日粮的陡坡牛的 DMI 比饲喂 IIR 日粮的陡坡牛高(P = 0.03)。然而,饲喂 EFC 青贮饲料或谷物不会影响背景饲养和育成期的体重、平均日增重和饲料效率。在育成阶段,与 EER 相比,饲喂 EEW 会降低(P ≤ 0.05)体重、平均日增重和饲料效率,并有降低(P = 0.06)热胴体重量的趋势。与只饲喂 IIR 的阉牛相比,饲喂 EIR 的阉牛在后备阶段单位 DMI 甲烷产量降低(P = 0.02)。饲喂 EFC 谷物对两个阶段的甲烷产量(克/天)均无影响。总之,在当前实验条件下,饲喂 EFC 青贮饲料或谷物并不能提高肉牛在背景饲养和育成阶段的生产性能。在背景阶段,饲喂 EFC 青贮饲料的肉牛单位 DMI 甲烷产量比饲喂隔离玉米青贮饲料的肉牛单位 DMI 甲烷产量要低。
{"title":"Effects of feeding α-amylase-expressed corn silage and grain on performance, enteric methane production, and carcass characteristics in beef steers","authors":"L. Rebelo, Kirsten L Clark, A. Relling, Chanhee Lee","doi":"10.1093/tas/txae080","DOIUrl":"https://doi.org/10.1093/tas/txae080","url":null,"abstract":"Abstract An experiment was conducted to evaluate the effects of feeding Enogen feed corn (EFC) silage or EFC grain with different grain processing (dry-rolled corn vs. whole-shelled corn) in feedlot cattle diets. Total 68 Angus cross-bred steers were blocked by body weight and the treatments (diets) were randomly assigned to steers in each block: a basal diet with isoline corn silage and isoline dry-rolled corn grain (IIR); the basal diet with EFC silage and isoline dry-rolled corn grain (EIR); the basal diet with EFC silage and EFC dry-rolled grain (EER); and the basal diet with EFC silage and EFC whole-shelled grain (EEW). Isoline refers to the isogenic counterpart of Enogen corn silage or grain. Steers received the assigned treatment over 32 wk of the entire experiment (backgrounding and finishing) until harvested. Part of the steers (eight blocks) in each treatment were used to measure CH4 production (g/d) using the GreenFeed and CH4 production per unit of DMI. All data were analyzed using a mixed procedure of SAS in a randomized complete block design, considering diet as a fixed effect and block as a random effect. Steers fed the EIR diet increased (P = 0.03) DMI compared to IIR during the backgrounding phase. However, feeding EFC silage or grain did not affect body weight, average daily gain, and feed efficiency during backgrounding and finishing phases. Feeding EEW decreased (P ≤ 0.05) body weight, average daily gain, feed efficiency, and tended to decrease (P = 0.06) hot carcass weight compared to EER during the finishing phase. Methane production per unit of DMI decreased (P = 0.02) for steers fed EIR compared with steers fed IIR only during the backgrounding phase. Feeding EFC grain had no effect on CH4 production (g/d) in both phases. In conclusion, feeding EFC silage or grain did not improve the performance of beef steers during the backgrounding and finishing phases in the current experiment condition. Methane production per unit of DMI was reduced for steers fed EFC silage compared with isoline corn silage only during the backgrounding phase.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lessons learned from using wild-caught and captive-reared lesser scaup (Aythya affinis) in captive experiments 在人工饲养实验中使用野生捕获和人工饲养的小红椋鸟(Aythya affinis)的经验教训
IF 1.3 Q2 Veterinary Pub Date : 2024-05-04 DOI: 10.1093/tas/txae076
Cheyenne R. Beach, Christopher N Jacques, J. D. Lancaster, Douglas C Osborne, A. Yetter, Rebecca A Cole, H. Hagy, Auriel M V Fournier
Abstract Waterfowl are housed in captivity for research studies that are infeasible in the wild. Accommodating the unique requirements of semi-aquatic species in captivity while meeting experimental design criteria for research questions can be challenging and may have unknown effects on animal health. Thus, testing and standardizing best husbandry and care practices for waterfowl is necessary to facilitate proper husbandry and humane care while ensuring reliable and repeatable research results. To inform husbandry practices for captive-reared and wild-caught lesser scaup (Aythya affinis; hereafter, scaup), we assessed body mass and fat composition across two different aspects of husbandry, source population (captive-reared or wild caught), and housing densities (birds/m2). Our results suggest that housing scaup at low densities (≤0.6 m2/bird, P = 0.049) relative to other species can minimize negative health effects. Captive-reared scaup were heavier (P = 0.027) with greater body fat (P < 0.001) and exhibited fewer signs of stress during handling than wild-caught scaup. In our experience, scaup which are captive-reared from eggs collected in the wild were better for long-term captivity studies as they maintained body mass between and recovered lost body mass following trials. Researchers would benefit from carefully evaluating the tradeoffs of using short- and long-term captive methods on their research question before designing projects, husbandry practices, and housing facilities for waterfowl.
摘要 水禽被圈养起来进行研究,这在野外是不可行的。既要满足研究问题的实验设计标准,又要满足人工饲养的半水生物种的独特要求,这可能具有挑战性,而且可能对动物健康产生未知的影响。因此,有必要对水禽的最佳饲养和护理方法进行测试和标准化,以促进适当的饲养和人道护理,同时确保研究结果的可靠性和可重复性。为了给人工饲养和野外捕获的小冠鸊(Aythya affinis,以下简称 "冠鸊")的饲养方法提供参考,我们评估了两个不同方面的饲养方法、源种群(人工饲养或野外捕获)和饲养密度(鸟/平方米)的体重和脂肪组成。我们的结果表明,与其他物种相比,以较低密度(≤0.6 m2/只,P = 0.049)饲养红椋鸟可最大限度地减少对健康的负面影响。人工饲养的红嘴鸊与野生捕捞的红嘴鸊相比,体重更大(P = 0.027),体脂更高(P < 0.001),在处理过程中表现出的应激迹象更少。根据我们的经验,用从野外采集的鸟蛋人工饲养的红椋鸟更适合进行长期人工饲养研究,因为它们能在两次试验之间保持体质量,并在试验后恢复失去的体质量。研究人员在设计项目、饲养方法和水禽饲养设施之前,应仔细评估使用短期和长期人工饲养方法对其研究问题的利弊得失。
{"title":"Lessons learned from using wild-caught and captive-reared lesser scaup (Aythya affinis) in captive experiments","authors":"Cheyenne R. Beach, Christopher N Jacques, J. D. Lancaster, Douglas C Osborne, A. Yetter, Rebecca A Cole, H. Hagy, Auriel M V Fournier","doi":"10.1093/tas/txae076","DOIUrl":"https://doi.org/10.1093/tas/txae076","url":null,"abstract":"Abstract Waterfowl are housed in captivity for research studies that are infeasible in the wild. Accommodating the unique requirements of semi-aquatic species in captivity while meeting experimental design criteria for research questions can be challenging and may have unknown effects on animal health. Thus, testing and standardizing best husbandry and care practices for waterfowl is necessary to facilitate proper husbandry and humane care while ensuring reliable and repeatable research results. To inform husbandry practices for captive-reared and wild-caught lesser scaup (Aythya affinis; hereafter, scaup), we assessed body mass and fat composition across two different aspects of husbandry, source population (captive-reared or wild caught), and housing densities (birds/m2). Our results suggest that housing scaup at low densities (≤0.6 m2/bird, P = 0.049) relative to other species can minimize negative health effects. Captive-reared scaup were heavier (P = 0.027) with greater body fat (P < 0.001) and exhibited fewer signs of stress during handling than wild-caught scaup. In our experience, scaup which are captive-reared from eggs collected in the wild were better for long-term captivity studies as they maintained body mass between and recovered lost body mass following trials. Researchers would benefit from carefully evaluating the tradeoffs of using short- and long-term captive methods on their research question before designing projects, husbandry practices, and housing facilities for waterfowl.","PeriodicalId":23272,"journal":{"name":"Translational Animal Science","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141014515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variations in colostrum metabolite profiles in association with sow parity 初乳代谢物谱的变化与母猪胎次的关系
IF 1.3 Q2 Veterinary Pub Date : 2024-05-03 DOI: 10.1093/tas/txae062
J. Vötterl, H. Schwartz-Zimmermann, F. Lerch, Fitra Yosi, Suchitra Sharma, Markus Aigensberger, Patrick M. Rennhofer, F. Berthiller, B. Metzler-Zebeli
Information about the full spectrum of metabolites present in porcine colostrum and factors that influence metabolite abundances is still incomplete. Parity number appears to modulate the concentration of single metabolites in colostrum. This study aimed to 1) characterize the metabolome composition and 2) assess the effect of parity on metabolite profiles in porcine colostrum. Sows (n = 20) were divided into three parity groups: A) sows in parity 1 and 2 (n = 8), B) sows in parity 3 and 4 (n = 6), and C) sows in parity 5 and 6 (n = 6). Colostrum was collected within 12 h after parturition. A total of 125 metabolites were identified using targeted reversed phase high-performance liquid chromatography tandem mass spectrometry and anion-exchange chromatography-high resolution mass spectrometry. Gas chromatography additionally identified 19 fatty acids (FA). Across parities, colostrum was rich in creatine and creatinine, 1,3-dioleyl-2-palmitatoylglycerol, 1,3-dipalmitoyl-2-oleoylglycerol and sialyllactose. Alterations in colostrum concentrations were found for 8 metabolites among parity groups (P < 0.05) but effects were not linear. For instance, colostrum from parity group C comprised 75.4% more valine but 15.7, 34.1 and 47.9% less citric, pyruvic and pyroglutamic acid, respectively, compared to group A (P < 0.05). By contrast, colostrum from parity group B contained 39.5% more spermidine than from group A (P < 0.05). Of the FA, C18:1, C16:0 and C18:2 n-6 were the main FA across parities. Parity affected four FA (C18:3n3, C14:1, C17:0ai and C17:1), including 43.1% less α-linolenic acid (C18:3n3) in colostrum from parity group C compared to groups A and B (P < 0.05). Signature feature ranking identified 1-stearoyl-2-hydroxy-sn-glycero-3-phosphatidylcholine and the secondary bile acid hyodeoxycholic acid as the most discriminative metabolites, showing a higher variable importance in the projection score in colostrum from parity group A than from groups B and C. Overall, results provided a comprehensive overview about the metabolome composition of sow colostrum. The consequences of the changes in colostrum metabolites with increasing parity for the nutrient supply of the piglets should be investigated in the future. The knowledge gained in this study could be used to optimize feeding strategies for sows.
有关猪初乳中存在的全部代谢物以及影响代谢物丰度的因素的信息仍不完整。胎次似乎能调节初乳中单一代谢物的浓度。本研究旨在:1)描述代谢组的组成;2)评估胎次对猪初乳中代谢物含量的影响。母猪(n = 20)被分为三个妊娠期组:A)1-2龄母猪(n = 8);B)3-4龄母猪(n = 6);C)5-6龄母猪(n = 6)。产后 12 小时内收集初乳。采用定向反相高效液相色谱串联质谱法和阴离子交换色谱-高分辨质谱法鉴定了 125 种代谢物。气相色谱法还鉴定了 19 种脂肪酸 (FA)。不同婴幼儿的初乳富含肌酸和肌酐、1,3-二油酰-2-棕榈酰甘油、1,3-二棕榈酰-2-油酰甘油和半乳糖。8种代谢物的初乳浓度在不同的雌雄胎次组间发生了变化(P < 0.05),但这种变化不是线性的。例如,与 A 组相比,C 组的初乳中缬氨酸含量高 75.4%,但柠檬酸、丙酮酸和焦谷氨酸含量分别低 15.7%、34.1% 和 47.9%(P < 0.05)。相比之下,奇数组 B 的初乳中精胺含量比 A 组多 39.5%(P < 0.05)。在各种FA中,C18:1、C16:0和C18:2 n-6是各奇数组的主要FA。奇偶性影响四种脂肪酸(C18:3n3、C14:1、C17:0ai 和 C17:1),其中奇偶性 C 组初乳中的α-亚麻酸(C18:3n3)比 A 组和 B 组少 43.1%(P < 0.05)。特征排序发现,1-硬脂酰基-2-羟基-sn-甘油-3-磷脂酰胆碱和次生胆汁酸透明脱氧胆酸是最具鉴别力的代谢物,与 B 组和 C 组相比,A 组母猪初乳在预测得分中显示出更高的变量重要性。今后应研究初乳代谢物随母猪奇数增加而发生的变化对仔猪营养供应的影响。本研究获得的知识可用于优化母猪的饲喂策略。
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引用次数: 0
Determining muscle plasticity and meat quality development of low-input extended fed market-ready steers. 确定低投入扩展饲喂适销对路阉牛的肌肉可塑性和肉质发展。
IF 1.3 Q2 Veterinary Pub Date : 2024-05-02 eCollection Date: 2024-01-01 DOI: 10.1093/tas/txae064
Jordan C Wicks, Alexis L Wivell, Mariane Beline, Morgan D Zumbaugh, Jocelyn S Bodmer, Con-Ning Yen, Chantal Johnson-Schuster, Thomas B Wilson, Scott P Greiner, Sally E Johnson, Tim H Shi, Saulo Luz Silva, David E Gerrard

In March 2020, the World Health Organization declared COVID-19 a pandemic, which ultimately led to many meat processors temporarily shutting down or reducing processing capacity. This backlog in processing capacity forced many feedlots to retain cattle for longer periods of time and assume the risk of major market fluctuations. The aim of this study was to understand how a dietary insult affects meat quality and muscle metabolism in market-ready steers (590 kg). Sixteen market-ready (590 kg) commercial Angus crossbred steers were subjected to a maintenance diet of either forage or grain for 60 d. Longissimus lumborum (LL) muscle samples were collected immediately postmortem and processed for characteristics reflecting the underlying muscle fiber type and energy state of the tissue. Despite cattle being subjected to a 60-d feeding period, there were no detectable differences (P > 0.05) in carcass characteristics, color of lean, or ultimate pH (pHu). Moreover, our data show that muscle plasticity is rather resilient, as reflected by lack of significance (P > 0.05) in oxidative and glycolytic enzymes, myosin heavy chain isoforms (MyHC), myoglobin, and mitochondrial DNA (mtDNA) contents. These data show that market-ready steers are capable of withstanding a low-input feeding strategy up to 60 d without dramatically impacting underlying muscle characteristics and meat quality development.

2020 年 3 月,世界卫生组织宣布 COVID-19 为大流行病,最终导致许多肉类加工商暂时关闭或降低加工能力。加工能力的积压迫使许多饲养场延长牛的饲养时间,并承担市场大幅波动的风险。本研究的目的是了解日粮刺激如何影响可上市肉牛(590 千克)的肉质和肌肉代谢。对 16 头可上市(590 千克)的商品安格斯杂交母牛进行了为期 60 天的饲料或谷物饲养。在牛死后立即采集了腰长肌 (LL) 肌肉样本,并对反映肌肉纤维类型和组织能量状态的特征进行了处理。尽管牛的饲养期为 60 天,但在胴体特征、瘦肉颜色或最终 pH 值(pHu)方面没有发现差异(P > 0.05)。此外,我们的数据还表明,肌肉的可塑性相当强,这体现在氧化酶和糖酵解酶、肌球蛋白重链同工酶(MyHC)、肌红蛋白和线粒体 DNA(mtDNA)含量的差异不显著(P > 0.05)。这些数据表明,适销对路的阉牛能够承受长达 60 天的低投入饲喂策略,而不会对基本肌肉特征和肉质发展产生显著影响。
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Translational Animal Science
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