Pub Date : 2021-09-15DOI: 10.3191/THERMALMED.37.95
K. Ohtsuka
{"title":"HSF1 Promotes T Cell Lymphoblastic Leukemia Progression by Controlling Cellular Energy Metabolism and mTORC1 Activation","authors":"K. Ohtsuka","doi":"10.3191/THERMALMED.37.95","DOIUrl":"https://doi.org/10.3191/THERMALMED.37.95","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76990311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-15DOI: 10.3191/THERMALMED.37.108
Shinsuke Nomura, H. Tsujimoto, Y. Morimoto
{"title":"Direction for Development of Photothermal Therapy","authors":"Shinsuke Nomura, H. Tsujimoto, Y. Morimoto","doi":"10.3191/THERMALMED.37.108","DOIUrl":"https://doi.org/10.3191/THERMALMED.37.108","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75806839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-15DOI: 10.3191/THERMALMED.37.79
A. Yoneda, Y. Tamura
{"title":"Targeting of Collagen-specific Chaperone Heat Shock Protein 47 for Cancer Therapy","authors":"A. Yoneda, Y. Tamura","doi":"10.3191/THERMALMED.37.79","DOIUrl":"https://doi.org/10.3191/THERMALMED.37.79","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88253157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-05DOI: 10.3191/thermalmed.37.31
Soichiro Kawagoe, Eiichiro Mori, T. Saio
Molecular chaperones have been extensively studied as mediators in protein folding and quality control but are recently attracting increasing attention as regulators of liquid-liquid phase separation (LLPS). In this review, we summarize recent studies regarding molecular chaperones involved in LLPS regulation. We also provide a brief introduction of biophysical and structural studies on molecular chaperones in protein folding that depict how the molecular chaperones recognize client proteins and alter the folding process. Although little is known about the mechanism of regulation of LLPS, the understanding of molecular chaperones in protein folding will provide hints for LLPS regulation.
{"title":"Regulation of Liquid-Liquid Phase Separation by Molecular Chaperones","authors":"Soichiro Kawagoe, Eiichiro Mori, T. Saio","doi":"10.3191/thermalmed.37.31","DOIUrl":"https://doi.org/10.3191/thermalmed.37.31","url":null,"abstract":"Molecular chaperones have been extensively studied as mediators in protein folding and quality control but are recently attracting increasing attention as regulators of liquid-liquid phase separation (LLPS). In this review, we summarize recent studies regarding molecular chaperones involved in LLPS regulation. We also provide a brief introduction of biophysical and structural studies on molecular chaperones in protein folding that depict how the molecular chaperones recognize client proteins and alter the folding process. Although little is known about the mechanism of regulation of LLPS, the understanding of molecular chaperones in protein folding will provide hints for LLPS regulation.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82173962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-05DOI: 10.3191/thermalmed.37.45
Y. Iseki, Tsugumi Nishidate
This paper proposes a deep-learning temperature measurement method using ultrasound images that is based on the thermal dependance of local changes in the speed of sound. In this method, the temperature distribution is measured using a non-invasive image analysis technique. In a previous study, we found a temperature measurement accuracy of 1.0 °C or less. However, our previous method has some disadvantages. First, the image analysis parameters (e.g., the size of the template and the cross-correlation threshold) are empirically determined. Second, it is necessary to obtain the thermal constant ktissue according to the type of tissue and the analysis parameters. To overcome these problems, we propose a new method using deep-learning. This new method is divided into three steps. The first step is to determine the image analysis parameters from the ultrasound images using a convolutional neural network (CNN). The second step is to analyze the image using the estimated analysis parameters to obtain a normalized temperature distribution. The third step is to determine the thermal constant ktissue to calibrate the temperature increase using multi-layered perceptron (MLP). In this paper, first, we propose three types of image fusion methods to input the ultrasound images into the CNN. Comparing the results of the three methods, we determine the optimal CNN structure. Second, we determine the optimal MLP structure by changing the number of hidden layers and neurons. Finally, as described above, we obtain the temperature distribution. Our results indicate that the proposed deep-learning method can effectively provide non-invasive temperature measurements.
{"title":"A Deep-Learning Approach for Non-Invasive Temperature Measurements Using Ultrasound Images","authors":"Y. Iseki, Tsugumi Nishidate","doi":"10.3191/thermalmed.37.45","DOIUrl":"https://doi.org/10.3191/thermalmed.37.45","url":null,"abstract":"This paper proposes a deep-learning temperature measurement method using ultrasound images that is based on the thermal dependance of local changes in the speed of sound. In this method, the temperature distribution is measured using a non-invasive image analysis technique. In a previous study, we found a temperature measurement accuracy of 1.0 °C or less. However, our previous method has some disadvantages. First, the image analysis parameters (e.g., the size of the template and the cross-correlation threshold) are empirically determined. Second, it is necessary to obtain the thermal constant ktissue according to the type of tissue and the analysis parameters. To overcome these problems, we propose a new method using deep-learning. This new method is divided into three steps. The first step is to determine the image analysis parameters from the ultrasound images using a convolutional neural network (CNN). The second step is to analyze the image using the estimated analysis parameters to obtain a normalized temperature distribution. The third step is to determine the thermal constant ktissue to calibrate the temperature increase using multi-layered perceptron (MLP). In this paper, first, we propose three types of image fusion methods to input the ultrasound images into the CNN. Comparing the results of the three methods, we determine the optimal CNN structure. Second, we determine the optimal MLP structure by changing the number of hidden layers and neurons. Finally, as described above, we obtain the temperature distribution. Our results indicate that the proposed deep-learning method can effectively provide non-invasive temperature measurements.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84567885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.3191/THERMALMED.37.25
T. Morino, N. Kawai, T. Yasui
This communication to chief editor discussed category classification process of medical products originated from Japanese academic researches, showing that of authorsʼ product for example. US guideline in 2017, “Classification of Products as Drugs and Devices & Additional Products. Classification Issues: Guidance for Industry and FDA Staff. Final Guidance”, was refereed and key provisions for device definition were introduced. In US, medical products were defined as device when primary intended purpose of the product was not achieved through chemical action. Examples of FDAʼs device definition of two kinds of heat generating nanoparticles were shown as gold nanoparticles illustrated in the guideline and magnetite cationic lipid composite particles (formerly named magnetite cationic liposomes) originated from Japanese academic research. Concerning category classification process in Japan, necessity of detailed discussion between academia and administrative agency was issued in order to facilitate its technology transfer to Japanese industry.
{"title":"Significance of Category Classification of Novel Medical Products Originated from Japanese Academic Researches with Regards to Its Industrialization","authors":"T. Morino, N. Kawai, T. Yasui","doi":"10.3191/THERMALMED.37.25","DOIUrl":"https://doi.org/10.3191/THERMALMED.37.25","url":null,"abstract":"This communication to chief editor discussed category classification process of medical products originated from Japanese academic researches, showing that of authorsʼ product for example. US guideline in 2017, “Classification of Products as Drugs and Devices & Additional Products. Classification Issues: Guidance for Industry and FDA Staff. Final Guidance”, was refereed and key provisions for device definition were introduced. In US, medical products were defined as device when primary intended purpose of the product was not achieved through chemical action. Examples of FDAʼs device definition of two kinds of heat generating nanoparticles were shown as gold nanoparticles illustrated in the guideline and magnetite cationic lipid composite particles (formerly named magnetite cationic liposomes) originated from Japanese academic research. Concerning category classification process in Japan, necessity of detailed discussion between academia and administrative agency was issued in order to facilitate its technology transfer to Japanese industry.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86285512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kanamori, Tsutomu Sato, T. Shima, J. Saitoh, G. Andócs, T. Kondo
{"title":"“Oncothermia” (Modulated electro-hyperthermia)","authors":"M. Kanamori, Tsutomu Sato, T. Shima, J. Saitoh, G. Andócs, T. Kondo","doi":"10.3191/THERMALMED.37.1","DOIUrl":"https://doi.org/10.3191/THERMALMED.37.1","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80790107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.3191/THERMALMED.36.101
T. Morino, A. Ito, T. Etani, T. Naiki, N. Kawai, T. Yasui
: Solid cancer therapy based on necrosis induction with heat-generating nanoparticles has been developed in Japan. Heat was induced from intratumorally injected magnetite cationic lipid composite particles (MCL particles) by alternating magnetic field irradiations to kill cancer cells nearby located. In our previous report we have showed importance of heat dose index in vivo (J / cm 3 tumor volume) for tumor regression when 45 mg MCL particles were injected at a single site of 1.36 cm 3 tumor. Purpose of this study is to show rationale and utility of multiple site injections of MCL particles for treatment of large tumors more than 1.36 cm 3 . Rat mammary tumors were induced by 7,12-dimethylbenz ［ a ］ anthracene (DMBA) and tumors in range of 2.19 ～ 3.81 cm 3 were applied to treatment experiment. Treatment condition was designed to reproduce the heat generation condition of the 1.36 cm 3 tumor treatment at every multiple injection site in rat mammary tumors. Tumor volume divided by number of injection sites (cm 3 / site) was set to close to 1.36 cm 3 / site and 45 mg MCL particles were administered at multiple sites to keep even spaces among injection sites. Three irradiation conditions were set to give close heat dose in vivo (J / cm 3 ) of the 1.36 cm 3 tumor treatment. Treatment under designed conditions resulted in complete regression of rat tumors at 21 days after the treatment, showing theoretical validity of design procedures for the multiple site injections. Novel concept of necrosed tumor volume from an injection site (cm 3 / site) and its actual value under a standard injection condition of 45 mg-MCL / site were described and its use in clinic was discussed.
日本已经开发出一种基于发热纳米颗粒诱导坏死的实体癌症治疗方法。通过交变磁场照射，将磁性阳离子脂质复合颗粒(MCL颗粒)注入瘤内，诱导热杀死附近的癌细胞。在我们之前的报告中，我们已经证明了在1.36 cm 3肿瘤的单个部位注射45 mg MCL颗粒时，体内热剂量指数(J / cm 3肿瘤体积)对肿瘤消退的重要性。本研究的目的是展示多部位注射MCL颗粒治疗大于1.36 cm 3的大肿瘤的原理和效用。采用7,12-二甲基苯[a]蒽(DMBA)诱导大鼠乳腺肿瘤，肿瘤范围为2.19 ~ 3.81 cm 3。设计治疗条件，再现大鼠乳腺肿瘤各多发注射部位1.36 cm 3肿瘤治疗的产热情况。肿瘤体积除以注射部位数(cm 3 /位点)设置为接近1.36 cm 3 /位点，在多个部位施用45 mg MCL颗粒以保持注射部位之间的均匀空间。设置三种照射条件，给予1.36 cm 3肿瘤治疗的接近体内热剂量(J / cm 3)。在设计条件下的治疗导致大鼠肿瘤在治疗后21天完全消退，显示了多部位注射设计程序的理论有效性。本文描述了一个注射部位坏死肿瘤体积(立方厘米/个部位)的新概念及其在标准注射条件下45 mg-MCL /个部位的实际值，并讨论了其在临床中的应用。
{"title":"Heat Dose Based Large Tumor Treatment with Multiple Site Injections of Heat-generating Nanoparticles Dispersible within Tumor Tissue","authors":"T. Morino, A. Ito, T. Etani, T. Naiki, N. Kawai, T. Yasui","doi":"10.3191/THERMALMED.36.101","DOIUrl":"https://doi.org/10.3191/THERMALMED.36.101","url":null,"abstract":": Solid cancer therapy based on necrosis induction with heat-generating nanoparticles has been developed in Japan. Heat was induced from intratumorally injected magnetite cationic lipid composite particles (MCL particles) by alternating magnetic field irradiations to kill cancer cells nearby located. In our previous report we have showed importance of heat dose index in vivo (J / cm 3 tumor volume) for tumor regression when 45 mg MCL particles were injected at a single site of 1.36 cm 3 tumor. Purpose of this study is to show rationale and utility of multiple site injections of MCL particles for treatment of large tumors more than 1.36 cm 3 . Rat mammary tumors were induced by 7,12-dimethylbenz ［ a ］ anthracene (DMBA) and tumors in range of 2.19 ～ 3.81 cm 3 were applied to treatment experiment. Treatment condition was designed to reproduce the heat generation condition of the 1.36 cm 3 tumor treatment at every multiple injection site in rat mammary tumors. Tumor volume divided by number of injection sites (cm 3 / site) was set to close to 1.36 cm 3 / site and 45 mg MCL particles were administered at multiple sites to keep even spaces among injection sites. Three irradiation conditions were set to give close heat dose in vivo (J / cm 3 ) of the 1.36 cm 3 tumor treatment. Treatment under designed conditions resulted in complete regression of rat tumors at 21 days after the treatment, showing theoretical validity of design procedures for the multiple site injections. Novel concept of necrosed tumor volume from an injection site (cm 3 / site) and its actual value under a standard injection condition of 45 mg-MCL / site were described and its use in clinic was discussed.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79009706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.3191/THERMALMED.36.91
Y. Tabuchi, Tatsuya Yunoki
: While hyperthermia (HT) is a promising modality for cancer therapy, major difficulty with the use of HT is the development of thermotolerance due to the elevation of heat shock proteins (HSPs), which function as molecular chaperons. Among the HSPs, Hsp70 possesses cytoprotective activity and plays a critical role in the acquisition of thermotolerance. Upon heat stress, Hsp70 rapidly translocates from the cytoplasm to nucleus. Recently, the protein Hikeshi, also known as the gene product of C11orf73, has been shown to function as a nuclear import carrier of Hsp70 under heat-stress conditions. Knockdown of Hikeshi significantly enhances sensitivity to HT and mild HT in the presence — but not the absence — of heat-stress in human cancer cells. Moreover, upregulation of Hikeshi expression is observed in human gastric or renal cancer. It has also been suggested that functional defects leading to homozygosity for a missense mutation, p. Cys4Ser or p. Val54Leu, in Hikeshi cause leukoencephalopathy in Finnish or Ashkenazi-Jewish patients, respectively. This review summarizes the physiological and pathological roles of Hikeshi and discusses its potential as a target in HT therapy.
{"title":"Can be Hikeshi a Potential Target for Hyperthermic Therapy?","authors":"Y. Tabuchi, Tatsuya Yunoki","doi":"10.3191/THERMALMED.36.91","DOIUrl":"https://doi.org/10.3191/THERMALMED.36.91","url":null,"abstract":": While hyperthermia (HT) is a promising modality for cancer therapy, major difficulty with the use of HT is the development of thermotolerance due to the elevation of heat shock proteins (HSPs), which function as molecular chaperons. Among the HSPs, Hsp70 possesses cytoprotective activity and plays a critical role in the acquisition of thermotolerance. Upon heat stress, Hsp70 rapidly translocates from the cytoplasm to nucleus. Recently, the protein Hikeshi, also known as the gene product of C11orf73, has been shown to function as a nuclear import carrier of Hsp70 under heat-stress conditions. Knockdown of Hikeshi significantly enhances sensitivity to HT and mild HT in the presence — but not the absence — of heat-stress in human cancer cells. Moreover, upregulation of Hikeshi expression is observed in human gastric or renal cancer. It has also been suggested that functional defects leading to homozygosity for a missense mutation, p. Cys4Ser or p. Val54Leu, in Hikeshi cause leukoencephalopathy in Finnish or Ashkenazi-Jewish patients, respectively. This review summarizes the physiological and pathological roles of Hikeshi and discusses its potential as a target in HT therapy.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88224536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-25DOI: 10.3191/thermalmed.36.75
T. Kondo, R. Suzuki, N. Sasaki, M. Takiguchi
{"title":"New Development of Ultrasonic Cancer Therapy","authors":"T. Kondo, R. Suzuki, N. Sasaki, M. Takiguchi","doi":"10.3191/thermalmed.36.75","DOIUrl":"https://doi.org/10.3191/thermalmed.36.75","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84161300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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