In hyperthermia treatment for superficial tumors, covering the affected part is often required to avoid intolerable pain. Polyvinylidene chloride (PVDC) sheets have been used to protect the affected area; however, the influence of PVDC covering on the temperature distribution is still unclear. In this study, the temperature changes caused by PVDC covering in hyperthermia treatment using agar phantoms were evaluated. In warming experiments, 30-cmand 10-cm-diameter electrodes were applied for deep and superficial warming, respectively. To confirm the effect of covering, either half the top side or both phantom ends were covered with the PVDC sheets or dry gauzes. After warming with various covering conditions, the temperature distribution of the phantoms was observed with thermography. The temperature changes over time were also evaluated with a thermocouple thermometer set in the border region of the covering. In deep warming, the increase of heat was slightly inhibited by PVDC covering, which intensified in the uncovered area. This contrast was amplified in superficial warming. Compared with PVDC, covering with dry gauzes showed a significant decrease and increase of heat in the covered and uncovered areas, respectively. In the observation of the temperature changes over time, during deep warming, the PVDC covering lowered no more than -0.4°C and the gauze covering lowered no more than -1.4°C, compared to that without any covers, in 10 min. In superficial warming, the heat increase in the gauze covering reached +7.0°C in only 5 min. This report showed that partial covering under electrodes could decrease the heat in the covered area and increase in the uncovered area. Although the temperature changes were minimal in PVDC covering, certain conditions could amplify the remodeling of the temperature distribution. Considering such changes is required to safely perform hyperthermia treatment.
{"title":"Influence of Polyvinylidene Chloride Sheets on Temperature Distribution in Hyperthermia Treatment","authors":"Manabe Asami, T. Motomura, F. Inoue, H. Terashima","doi":"10.3191/THERMALMED.35.2","DOIUrl":"https://doi.org/10.3191/THERMALMED.35.2","url":null,"abstract":"In hyperthermia treatment for superficial tumors, covering the affected part is often required to avoid intolerable pain. Polyvinylidene chloride (PVDC) sheets have been used to protect the affected area; however, the influence of PVDC covering on the temperature distribution is still unclear. In this study, the temperature changes caused by PVDC covering in hyperthermia treatment using agar phantoms were evaluated. In warming experiments, 30-cmand 10-cm-diameter electrodes were applied for deep and superficial warming, respectively. To confirm the effect of covering, either half the top side or both phantom ends were covered with the PVDC sheets or dry gauzes. After warming with various covering conditions, the temperature distribution of the phantoms was observed with thermography. The temperature changes over time were also evaluated with a thermocouple thermometer set in the border region of the covering. In deep warming, the increase of heat was slightly inhibited by PVDC covering, which intensified in the uncovered area. This contrast was amplified in superficial warming. Compared with PVDC, covering with dry gauzes showed a significant decrease and increase of heat in the covered and uncovered areas, respectively. In the observation of the temperature changes over time, during deep warming, the PVDC covering lowered no more than -0.4°C and the gauze covering lowered no more than -1.4°C, compared to that without any covers, in 10 min. In superficial warming, the heat increase in the gauze covering reached +7.0°C in only 5 min. This report showed that partial covering under electrodes could decrease the heat in the covered area and increase in the uncovered area. Although the temperature changes were minimal in PVDC covering, certain conditions could amplify the remodeling of the temperature distribution. Considering such changes is required to safely perform hyperthermia treatment.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79029043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In our previous study, a non-invasive heating method was proposed which could utilize a re-entrant type resonant cavity applicator for deep-seated tumors. For the first time, a prototype heating system using a cylindrical resonant cavity applicator was developed, and results from the experimental heating of agar phantoms and animals were discussed. In the proposed method, as a whole human body was set in the cylindrical resonant cavity applicator to heat the targeted area, there was a risk of heating healthy human tissues. In the present paper, a new method using a rectangular resonant cavity is proposed to non-invasively heat the targeted area without physical contact to the subject. Dimensions of the rectangular resonant cavity applicator were 60 cm in height, 70 cm in width and 20 cm in length. In this method, since the targeted area is placed inside the rectangular resonant cavity applicator, the risk of heating the healthy tissue is minimized. Here, the measured temperature distributions of the heated agar phantom with the developed system was described. First, a setup of the proposed resonant cavity applicator was presented. Second, the results of heating human shaped agar phantom were presented. Finally, the results of computer simulations and experiments with the developed prototype heating system were discussed. From these results, it was found that the proposed rectangular resonant cavity applicator could be useful in controlling a small heated area without contact to the human body, and could be applicable for treating various tumors.
{"title":"Heating Characteristics of Developed Rectangular Resonant Cavity Applicator for Non-contact Hyperthermia Treatments","authors":"Y. Ichishima, Y. Shindo, Y. Iseki, Kazuo Kato","doi":"10.3191/THERMALMED.35.1","DOIUrl":"https://doi.org/10.3191/THERMALMED.35.1","url":null,"abstract":"In our previous study, a non-invasive heating method was proposed which could utilize a re-entrant type resonant cavity applicator for deep-seated tumors. For the first time, a prototype heating system using a cylindrical resonant cavity applicator was developed, and results from the experimental heating of agar phantoms and animals were discussed. In the proposed method, as a whole human body was set in the cylindrical resonant cavity applicator to heat the targeted area, there was a risk of heating healthy human tissues. In the present paper, a new method using a rectangular resonant cavity is proposed to non-invasively heat the targeted area without physical contact to the subject. Dimensions of the rectangular resonant cavity applicator were 60 cm in height, 70 cm in width and 20 cm in length. In this method, since the targeted area is placed inside the rectangular resonant cavity applicator, the risk of heating the healthy tissue is minimized. Here, the measured temperature distributions of the heated agar phantom with the developed system was described. First, a setup of the proposed resonant cavity applicator was presented. Second, the results of heating human shaped agar phantom were presented. Finally, the results of computer simulations and experiments with the developed prototype heating system were discussed. From these results, it was found that the proposed rectangular resonant cavity applicator could be useful in controlling a small heated area without contact to the human body, and could be applicable for treating various tumors.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82981822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.3191/THERMALMED.34.53
Y. Iseki, Y. Shindo, K. Saito, Kazuo Kato
: This study describes the temperature properties of the radio frequency ( RF ) capacitive applicator with magnetic nanoparticles. In a clinic, two types of heating devices are most commonly used. One among these heating devices is a dielectric heating applicator, whereas the other type is an induction heating applicator. One of the disadvantages of dielectric heating applicators is their tendency to overheat the fat layers. Further, a cooling system is attached to reduce overheating. However, overheating remains one of most significant disadvantages of a dielectric applicator. In contrast, it is difficult to produce localized heating energy to the deep-seated tumors using induction heating applicators. To overcome these problems, we propose a method for using magnetic nanoparticles combined with an RF capacitive applicator. Further, the effectiveness of the proposed method has been examined by performing computer simulations and heating experiments using our prototype RF capacitive applicator. This study describes the temperature properties that are associated with the usage of magnetic nanoparticles and a hyperthermia applicator. First, the characteristics of a dielectric heating device and an induction heating device are described. Second, the electric properties of magnetic nanoparticles that exhibit concentrations ranging from 20 to 60 mg / cm 3 are measured in the frequency range from 100 MHz to 1.0 GHz, further, the temperature properties of the RF capacitive applicator with magnetic nanoparticles are calculated using the finite element method (FEM). Finally, the heating experiments are conducted using our prototype RF capacitive applicator and infrared thermal camera. These results of this study indicated that dielectric heating was the dominant heating mechanism in case of an RF capacitive applicator with magnetic nanoparticles. Additionally, it was suggested that the usage of magnetic nanoparticles will make it possible to control the heated area inside a patient ʼ s body. Thus, we observed that it was possible to use magnetic nanoparticles for performing effective hyperthermia treatment based on the results of both computer simulations and heating experiments.
{"title":"Heating Properties of RF Capacitive Applicator with Magnetic Nanoparticles","authors":"Y. Iseki, Y. Shindo, K. Saito, Kazuo Kato","doi":"10.3191/THERMALMED.34.53","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.53","url":null,"abstract":": This study describes the temperature properties of the radio frequency ( RF ) capacitive applicator with magnetic nanoparticles. In a clinic, two types of heating devices are most commonly used. One among these heating devices is a dielectric heating applicator, whereas the other type is an induction heating applicator. One of the disadvantages of dielectric heating applicators is their tendency to overheat the fat layers. Further, a cooling system is attached to reduce overheating. However, overheating remains one of most significant disadvantages of a dielectric applicator. In contrast, it is difficult to produce localized heating energy to the deep-seated tumors using induction heating applicators. To overcome these problems, we propose a method for using magnetic nanoparticles combined with an RF capacitive applicator. Further, the effectiveness of the proposed method has been examined by performing computer simulations and heating experiments using our prototype RF capacitive applicator. This study describes the temperature properties that are associated with the usage of magnetic nanoparticles and a hyperthermia applicator. First, the characteristics of a dielectric heating device and an induction heating device are described. Second, the electric properties of magnetic nanoparticles that exhibit concentrations ranging from 20 to 60 mg / cm 3 are measured in the frequency range from 100 MHz to 1.0 GHz, further, the temperature properties of the RF capacitive applicator with magnetic nanoparticles are calculated using the finite element method (FEM). Finally, the heating experiments are conducted using our prototype RF capacitive applicator and infrared thermal camera. These results of this study indicated that dielectric heating was the dominant heating mechanism in case of an RF capacitive applicator with magnetic nanoparticles. Additionally, it was suggested that the usage of magnetic nanoparticles will make it possible to control the heated area inside a patient ʼ s body. Thus, we observed that it was possible to use magnetic nanoparticles for performing effective hyperthermia treatment based on the results of both computer simulations and heating experiments.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81782898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-15DOI: 10.3191/THERMALMED.34.35
T. Ishikawa, T. Okayama, Naoyuki Sakamoto, S. Kokura, T. Yoshikawa
{"title":"A Review of Regional Hyperthermia for Digestive Cancers: Current Status and Future Directions","authors":"T. Ishikawa, T. Okayama, Naoyuki Sakamoto, S. Kokura, T. Yoshikawa","doi":"10.3191/THERMALMED.34.35","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.35","url":null,"abstract":"","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75864225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-15DOI: 10.3191/THERMALMED.34.23
Yuichi Miyoshi, Kazunori Watanabe
: Mammal possesses mechanisms that respond to environmental stresses, including heat, oxidation, radiation. Stress responses include cell death induction mechanism such as apoptosis and stress accommodation mechanism for survival. One of a major stress accommodation mechanism is formation of stress granules (SGs) and nuclear stress bodies (nSBs). SGs and nSBs, which are constituted by many proteins and RNAs, are reversible intracellular structures formed only when cells are exposed to environmental stresses. SGs, formed in the cytoplasm, have been found in a wide range of eukaryotes from yeast to humans. Intriguingly, nSBs, formed in the nucleus, have been found only in human cells. In this review, we focus on nSBs. nSBs were discovered in heat-stressed cells in 1989, and then many proteins and RNAs have been identified. Major components of nSBs are heat shock transcription factor family, splicing factors and noncoding RNAs (Satellite III RNA and initiator / elongator tRNA). Recently, many researchers have reported the formation mechanism of nSBs, however cellular functions of nSBs remain unclear. In this review, we introduce the basic researches focusing on the nSBs formation mechanism and cellular functions of nSBs constitution factors.
哺乳动物具有对环境压力作出反应的机制，包括热、氧化、辐射。应激反应包括细胞凋亡等诱导死亡机制和生存的应激调节机制。应力颗粒(SGs)和核应力体(nsb)的形成是应力调节的主要机制之一。SGs和nsb是由多种蛋白质和rna组成的可逆细胞内结构，只有在细胞受到环境胁迫时才会形成。在细胞质中形成的SGs，已经在从酵母到人类的许多真核生物中发现。有趣的是，在细胞核中形成的nsb只在人类细胞中被发现。在这篇综述中，我们主要关注nsb。nsb于1989年在热应激细胞中被发现，随后被鉴定出许多蛋白质和rna。nsb的主要成分是热休克转录因子家族、剪接因子和非编码RNA (Satellite III RNA和启动子/延长子tRNA)。近年来，许多研究者报道了非nsb的形成机制，但其细胞功能尚不清楚。本文主要介绍了非甾体抗体形成机制和非甾体抗体构成因子的细胞功能等方面的研究进展。
{"title":"Formation Mechanism and Cellular Functions of Nuclear Stress Bodies Induced by Heat Stress","authors":"Yuichi Miyoshi, Kazunori Watanabe","doi":"10.3191/THERMALMED.34.23","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.23","url":null,"abstract":": Mammal possesses mechanisms that respond to environmental stresses, including heat, oxidation, radiation. Stress responses include cell death induction mechanism such as apoptosis and stress accommodation mechanism for survival. One of a major stress accommodation mechanism is formation of stress granules (SGs) and nuclear stress bodies (nSBs). SGs and nSBs, which are constituted by many proteins and RNAs, are reversible intracellular structures formed only when cells are exposed to environmental stresses. SGs, formed in the cytoplasm, have been found in a wide range of eukaryotes from yeast to humans. Intriguingly, nSBs, formed in the nucleus, have been found only in human cells. In this review, we focus on nSBs. nSBs were discovered in heat-stressed cells in 1989, and then many proteins and RNAs have been identified. Major components of nSBs are heat shock transcription factor family, splicing factors and noncoding RNAs (Satellite III RNA and initiator / elongator tRNA). Recently, many researchers have reported the formation mechanism of nSBs, however cellular functions of nSBs remain unclear. In this review, we introduce the basic researches focusing on the nSBs formation mechanism and cellular functions of nSBs constitution factors.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73343879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-15DOI: 10.3191/THERMALMED.34.45
Y. Yoshida, S. Tominaga, Liqiu Ma, A. Takahashi
: Tumor hypoxia is a negative prognostic and predictive factor for radiotherapy, and hyperthermia therapy is clinically useful for overcoming radioresistance in hypoxic tumors. However, the mechanism for the hyperthermia-induced cell death observed in hypoxic tumors remains unknown. We aimed to clarify the relationship between heat sensitivity and heat-induced DNA double-strand breaks (DSBs), reflecting DNA damage, in tumor cells under hypoxia. HeLa human cervical epithelial adenocarcinoma cells were subjected to heat treatment or X-ray irradiation under hypoxia or normoxia. Control cells were left untreated. The formation of DSBs was evaluated by immunocytochemistry for histone γ H2AX foci, given that one histone γ H2AX focus reflects one DSB. Cell survival was evaluated by colony-formation assays. The colony-formation assays revealed that hypoxic cells showed greater radioresistance, as expected, but only slightly higher heat resistance than normoxic cells. Under normoxia, heat-treated or X-ray-irradiated cells showed larger amounts of γ H2AX foci formation than control cells, reflecting increased DSB formation and more DNA damage. Under hypoxia, heat-treated cells showed a less remarkable decrease in γ H2AX foci formation than X-ray-irradiated cells, reflecting sustained levels of DSB formation and DNA damage. The present findings indicate that heat treatment can induce DNA damage via DSB formation reflected by γ H2AX foci formation under hypoxia. The findings provide further support for an important role of heat-induced DSB damage in cell killing in hypoxic tumors that show radioresistance. Hyperthermia therapy can be beneficial for the prognosis of cancer patients through increased DNA damage leading to tumor cell death.
{"title":"Heat Induces Histone γH2AX Formation under Hypoxia","authors":"Y. Yoshida, S. Tominaga, Liqiu Ma, A. Takahashi","doi":"10.3191/THERMALMED.34.45","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.45","url":null,"abstract":": Tumor hypoxia is a negative prognostic and predictive factor for radiotherapy, and hyperthermia therapy is clinically useful for overcoming radioresistance in hypoxic tumors. However, the mechanism for the hyperthermia-induced cell death observed in hypoxic tumors remains unknown. We aimed to clarify the relationship between heat sensitivity and heat-induced DNA double-strand breaks (DSBs), reflecting DNA damage, in tumor cells under hypoxia. HeLa human cervical epithelial adenocarcinoma cells were subjected to heat treatment or X-ray irradiation under hypoxia or normoxia. Control cells were left untreated. The formation of DSBs was evaluated by immunocytochemistry for histone γ H2AX foci, given that one histone γ H2AX focus reflects one DSB. Cell survival was evaluated by colony-formation assays. The colony-formation assays revealed that hypoxic cells showed greater radioresistance, as expected, but only slightly higher heat resistance than normoxic cells. Under normoxia, heat-treated or X-ray-irradiated cells showed larger amounts of γ H2AX foci formation than control cells, reflecting increased DSB formation and more DNA damage. Under hypoxia, heat-treated cells showed a less remarkable decrease in γ H2AX foci formation than X-ray-irradiated cells, reflecting sustained levels of DSB formation and DNA damage. The present findings indicate that heat treatment can induce DNA damage via DSB formation reflected by γ H2AX foci formation under hypoxia. The findings provide further support for an important role of heat-induced DSB damage in cell killing in hypoxic tumors that show radioresistance. Hyperthermia therapy can be beneficial for the prognosis of cancer patients through increased DNA damage leading to tumor cell death.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85277145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-15DOI: 10.3191/THERMALMED.34.15
Y. Nakagawa, A. Kajihara, T. Kirita, Eiichiro Mori
Hyperthermia is generally used in combination with chemo and radiation therapy in the treatment of various cancers. Thus far, most studies have focused on the additive effects of heat shock. However, it is also critical to understand the solitary effect of heat shock stress on mammalian cells. DNA double-strand breaks (DSBs) are known to be generated by ionizing radiation and a variety of DNA modifying reagents. As shown by neutral comet assays and γH2AX (phosphorylated histone H2AX at serine 139) focus formation, heat shock also induces DSBs. While existing literature suggests that heat shock leads to cell death through the induction of DSBs, the pathway involved in repairing heat-induced damage remains to be elucidated. In the current review, we examined the history of hyperthermia, from the discovery of DSBs after heat shock, to our recent finding regarding the homologous recombination repair pathway after heat shock.
{"title":"Heat Meets DNA: DNA Damage and Repair","authors":"Y. Nakagawa, A. Kajihara, T. Kirita, Eiichiro Mori","doi":"10.3191/THERMALMED.34.15","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.15","url":null,"abstract":"Hyperthermia is generally used in combination with chemo and radiation therapy in the treatment of various cancers. Thus far, most studies have focused on the additive effects of heat shock. However, it is also critical to understand the solitary effect of heat shock stress on mammalian cells. DNA double-strand breaks (DSBs) are known to be generated by ionizing radiation and a variety of DNA modifying reagents. As shown by neutral comet assays and γH2AX (phosphorylated histone H2AX at serine 139) focus formation, heat shock also induces DSBs. While existing literature suggests that heat shock leads to cell death through the induction of DSBs, the pathway involved in repairing heat-induced damage remains to be elucidated. In the current review, we examined the history of hyperthermia, from the discovery of DSBs after heat shock, to our recent finding regarding the homologous recombination repair pathway after heat shock.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89265845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A R T I C L E I N F O A B S T R A C T Article history: Received: 16 September, 2020 Accepted: 01 December, 2020 Online: 16 December, 2020 High Intensity Focused Ultrasound (HIFU) was widely used for treating tumors noninvasively. In this treatment, ultrasound is focused on the target volume inside the human body to ablate cancerous tissues and Magnetic Resonance Imaging (MRI) is mainly used to grasp the target position and to measure the temperature distributions around the target. However, MRI is very expensive, and a large space is required. In this paper, we presented a method for measuring the temperature distribution using an ultrasound diagnostic device, which is inexpensive and commonly used in many clinics, and actually showed the results of heating experiments on a human shaped agar phantom. The proposed method for measuring the temperature distribution around the heated target was conducted by performing image processing on two ultrasound images before and after heating. Furthermore, it was confirmed that it was possible to grasp the three-dimensional temperature distribution from the images in multiple layers. The effectiveness of the temperature distribution measurement results by the proposed method was shown by comparing the temperature measurement results with the infrared thermal camera. The error between the results was approximately 1 °C. It was found that the non-invasive measurement method of the three-dimensional temperature distribution around the target volume using the ultrasound images was useful for effective HIFU treatments.
A R T I C L E I N F O A B S T R A C T文章历史:收稿日期:2020年9月16日接收日期:2020年12月01日发布日期:2020年12月16日高强度聚焦超声(High Intensity Focused Ultrasound, HIFU)被广泛应用于肿瘤的无创治疗。在这种治疗中，超声聚焦于人体内部的靶体积，以消融癌组织，磁共振成像(MRI)主要用于掌握靶的位置和测量靶周围的温度分布。然而，MRI非常昂贵，并且需要很大的空间。在本文中，我们提出了一种使用超声诊断设备测量温度分布的方法，这种设备价格低廉，在许多诊所中都很常用，并实际展示了在人形琼脂体上加热实验的结果。所提出的测量被加热目标周围温度分布的方法是通过对加热前后的两张超声图像进行图像处理来实现的。进一步证实了从多层图像中掌握三维温度分布是可能的。通过与红外热像仪测温结果的比较，验证了该方法测温结果的有效性。结果之间的误差约为1°C。发现利用超声图像无创测量靶体周围三维温度分布的方法有助于有效的HIFU治疗。
{"title":"Basic Study of 3-D Non-Invasive Measurement of Temperature Distribution Using Ultrasound Images during FUS Heating","authors":"Eitaro Miura, Kazuo Kato, A. Takeuchi","doi":"10.3191/THERMALMED.34.1","DOIUrl":"https://doi.org/10.3191/THERMALMED.34.1","url":null,"abstract":"A R T I C L E I N F O A B S T R A C T Article history: Received: 16 September, 2020 Accepted: 01 December, 2020 Online: 16 December, 2020 High Intensity Focused Ultrasound (HIFU) was widely used for treating tumors noninvasively. In this treatment, ultrasound is focused on the target volume inside the human body to ablate cancerous tissues and Magnetic Resonance Imaging (MRI) is mainly used to grasp the target position and to measure the temperature distributions around the target. However, MRI is very expensive, and a large space is required. In this paper, we presented a method for measuring the temperature distribution using an ultrasound diagnostic device, which is inexpensive and commonly used in many clinics, and actually showed the results of heating experiments on a human shaped agar phantom. The proposed method for measuring the temperature distribution around the heated target was conducted by performing image processing on two ultrasound images before and after heating. Furthermore, it was confirmed that it was possible to grasp the three-dimensional temperature distribution from the images in multiple layers. The effectiveness of the temperature distribution measurement results by the proposed method was shown by comparing the temperature measurement results with the infrared thermal camera. The error between the results was approximately 1 °C. It was found that the non-invasive measurement method of the three-dimensional temperature distribution around the target volume using the ultrasound images was useful for effective HIFU treatments.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89220533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.3191/THERMALMED.33.91
Y. Shindo, Kenji Takahashi, F. Ikuta, Y. Iseki, Kazuo Kato
This paper describes an improved deep thermal osteoarthritis (OA) rehabilitation system which has a temperature distribution measurement system. In a previous study, we developed a resonant cavity applicator for treating OA and proved that this system was able to heat the knee joint more effectively than other methods; however, this heating system did not have a temperature measurement function. Meanwhile, in a different study, we developed a method using ultrasound imaging techniques to calculate temperature distributions inside the human body. In consideration of clinical application of this applicator, it is necessary to be able to measure the temperature of human tissue during heating. With our applicator, the most important thing is to create an electromagnetic resonant mode deep inside the cavity. Unfortunately, because of electromagnetic interference, we could not utilize an ultrasound imaging probe inside the cavity during heating. To overcome this critical problem, we developed a heating system with a new temperature measurement system. We designed an original jig made of PTFE and developed the remote controllable robotic arm to properly position the probe to take ultrasound images as precisely as possible. Furthermore, the resonant cavity applicator was modified so that it was able to house the ultrasound imaging probe during the heating treatment. In this paper, we first evaluate the performance of the jig by comparing displacement vector distributions. Second, we discuss the results of a heating experiment using this prototype applicator. From our results, it was found that our thermal rehabilitation system with the added temperature measurement function would be useful in clinics for treating osteoarthritis inside the knee joint.
{"title":"Improved Deep Thermal Rehabilitation System with Temperature Measurement Function Using Ultrasound Images","authors":"Y. Shindo, Kenji Takahashi, F. Ikuta, Y. Iseki, Kazuo Kato","doi":"10.3191/THERMALMED.33.91","DOIUrl":"https://doi.org/10.3191/THERMALMED.33.91","url":null,"abstract":"This paper describes an improved deep thermal osteoarthritis (OA) rehabilitation system which has a temperature distribution measurement system. In a previous study, we developed a resonant cavity applicator for treating OA and proved that this system was able to heat the knee joint more effectively than other methods; however, this heating system did not have a temperature measurement function. Meanwhile, in a different study, we developed a method using ultrasound imaging techniques to calculate temperature distributions inside the human body. In consideration of clinical application of this applicator, it is necessary to be able to measure the temperature of human tissue during heating. With our applicator, the most important thing is to create an electromagnetic resonant mode deep inside the cavity. Unfortunately, because of electromagnetic interference, we could not utilize an ultrasound imaging probe inside the cavity during heating. To overcome this critical problem, we developed a heating system with a new temperature measurement system. We designed an original jig made of PTFE and developed the remote controllable robotic arm to properly position the probe to take ultrasound images as precisely as possible. Furthermore, the resonant cavity applicator was modified so that it was able to house the ultrasound imaging probe during the heating treatment. In this paper, we first evaluate the performance of the jig by comparing displacement vector distributions. Second, we discuss the results of a heating experiment using this prototype applicator. From our results, it was found that our thermal rehabilitation system with the added temperature measurement function would be useful in clinics for treating osteoarthritis inside the knee joint.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87929872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.3191/THERMALMED.33.39
Akiko Ohki, Minori Tanoue, Sayumi Kobayashi, K. Murase
This study was undertaken to evaluate the tumor response to magnetic hyperthermia treatment (MHT) combined with cisplatin (MHT+CDDP) using magnetic particle imaging (MPI). Colon-26 cells were implanted into the backs of mice. When the tumor volume exceeded approximately 100 mm3, the mice were divided into control, MHT, CDDP, and MHT+CDDP groups. In the CDDP and MHT+CDDP groups, CDDP (5 mg/kg) was injected intraperitoneally. In the MHT+CDDP group, magnetic nanoparticles [250 mM (14.0 mg Fe/mL) Resovist®]were directly injected into the tumor one hour after CDDP administration, and MHT was performed for 20 min using an alternating magnetic field. In the MHT group, only MHT was performed after the injection of Resovist®. In the MHT+CDDP and MHT groups, MPI images were obtained using our MPI scanner immediately before, immediately after, and 3, 7, and 14 days after MHT. After the MPI studies, we drew a region of interest (ROI) on the tumor in the MPI image and calculated the average and maximum MPI values and the number of pixels within the ROI. In all groups, the relative tumor volume growth (RTVG) was calculated from (V-V0)/V0, where V0 and V were the tumor volumes immediately before and after treatment, respectively. The RTVG value in the MHT+CDDP group was significantly lower than that in the MHT group 3 to 14 days after MHT. It was also significantly lower than that in the CDDP group at 4 to 11 days except at 6 and 9 days after treatment. The average and maximum MPI values normalized by those immediately before MHT in the MHT+CDDP group were significantly higher than those in the MHT group 3 days after MHT. Our results suggested that MPI is useful for quantitatively evaluating tumor response to MHT combined with chemotherapy.
{"title":"Magnetic Particle Imaging for Quantitative Evaluation of Tumor Response to Magnetic Hyperthermia Treatment Combined with Chemotherapy Using Cisplatin","authors":"Akiko Ohki, Minori Tanoue, Sayumi Kobayashi, K. Murase","doi":"10.3191/THERMALMED.33.39","DOIUrl":"https://doi.org/10.3191/THERMALMED.33.39","url":null,"abstract":"This study was undertaken to evaluate the tumor response to magnetic hyperthermia treatment (MHT) combined with cisplatin (MHT+CDDP) using magnetic particle imaging (MPI). Colon-26 cells were implanted into the backs of mice. When the tumor volume exceeded approximately 100 mm3, the mice were divided into control, MHT, CDDP, and MHT+CDDP groups. In the CDDP and MHT+CDDP groups, CDDP (5 mg/kg) was injected intraperitoneally. In the MHT+CDDP group, magnetic nanoparticles [250 mM (14.0 mg Fe/mL) Resovist®]were directly injected into the tumor one hour after CDDP administration, and MHT was performed for 20 min using an alternating magnetic field. In the MHT group, only MHT was performed after the injection of Resovist®. In the MHT+CDDP and MHT groups, MPI images were obtained using our MPI scanner immediately before, immediately after, and 3, 7, and 14 days after MHT. After the MPI studies, we drew a region of interest (ROI) on the tumor in the MPI image and calculated the average and maximum MPI values and the number of pixels within the ROI. In all groups, the relative tumor volume growth (RTVG) was calculated from (V-V0)/V0, where V0 and V were the tumor volumes immediately before and after treatment, respectively. The RTVG value in the MHT+CDDP group was significantly lower than that in the MHT group 3 to 14 days after MHT. It was also significantly lower than that in the CDDP group at 4 to 11 days except at 6 and 9 days after treatment. The average and maximum MPI values normalized by those immediately before MHT in the MHT+CDDP group were significantly higher than those in the MHT group 3 days after MHT. Our results suggested that MPI is useful for quantitatively evaluating tumor response to MHT combined with chemotherapy.","PeriodicalId":23299,"journal":{"name":"Thermal Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88123186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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