Engin Kölükçü, V. Unsal, F. Fırat, F. Gevrek, M. Katar
We aimed to analyze the effects of diacerein in a rat model of partial unilateral ureteral obstruction (PUUO). We randomly divided 24 female rats into three groups. Control group, PUUO group and PUUO + diacerein group. The PUUO group was subjected to the PUUO model for seven days. The PUUO + diacerein group received oral diacerein (80 mg/kg) for seven days. Spectrophotometric methods were employed to measure oxidative stress parameters, including malondialdehyde (MDA), protein carbonyl (PC) and antioxidant enzyme levels, including glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), while indicators of renal function, such as kidney injury molecule-1 (KIM-1) and neutrophil gelatinous-associated lipocalin (NGAL), along with inflammatory parameters interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), were assessed using the ELISA method. Inflammatory parameters were measured in blood samples, and other parameters were analyzed in kidney tissue. Hematoxylin-eosin method examinations were used for histological analyses. IL-1beta, TNF-alpha, and IL-6 levels were found to be significantly decreased in the PUUO + diacerein group compared to the PUUO group (p=0.006, p=0.002 and p=0.001, respectively). In the PUUO + diacerein group, GSH-Px and SOD activities increased compared with the PUUO group (p=0.031 and p=0.037, respectively). We also observed a significant improvement in renal function parameters, such as KIM-1 and NGAL levels in the PUUO + diacerein group compared to PUUO (p=0.002 and p=0.012, respectively). The PC and MDA levels were highest in the PUUO group (p<0.001). Similarly, the histopathologic tissue damage was the most prominent PUUO group (p<0.001). Our study found that diacerein is a highly effective pharmacologic agent in alleviating oxidative damage in PUUO model rats.
{"title":"Renoprotective effect of diacerein in rats with partial unilateral ureteral obstruction model","authors":"Engin Kölükçü, V. Unsal, F. Fırat, F. Gevrek, M. Katar","doi":"10.1515/tjb-2023-0237","DOIUrl":"https://doi.org/10.1515/tjb-2023-0237","url":null,"abstract":"\u0000 \u0000 \u0000 We aimed to analyze the effects of diacerein in a rat model of partial unilateral ureteral obstruction (PUUO).\u0000 \u0000 \u0000 \u0000 We randomly divided 24 female rats into three groups. Control group, PUUO group and PUUO + diacerein group. The PUUO group was subjected to the PUUO model for seven days. The PUUO + diacerein group received oral diacerein (80 mg/kg) for seven days. Spectrophotometric methods were employed to measure oxidative stress parameters, including malondialdehyde (MDA), protein carbonyl (PC) and antioxidant enzyme levels, including glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), while indicators of renal function, such as kidney injury molecule-1 (KIM-1) and neutrophil gelatinous-associated lipocalin (NGAL), along with inflammatory parameters interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), were assessed using the ELISA method. Inflammatory parameters were measured in blood samples, and other parameters were analyzed in kidney tissue. Hematoxylin-eosin method examinations were used for histological analyses.\u0000 \u0000 \u0000 \u0000 IL-1beta, TNF-alpha, and IL-6 levels were found to be significantly decreased in the PUUO + diacerein group compared to the PUUO group (p=0.006, p=0.002 and p=0.001, respectively). In the PUUO + diacerein group, GSH-Px and SOD activities increased compared with the PUUO group (p=0.031 and p=0.037, respectively). We also observed a significant improvement in renal function parameters, such as KIM-1 and NGAL levels in the PUUO + diacerein group compared to PUUO (p=0.002 and p=0.012, respectively). The PC and MDA levels were highest in the PUUO group (p<0.001). Similarly, the histopathologic tissue damage was the most prominent PUUO group (p<0.001).\u0000 \u0000 \u0000 \u0000 Our study found that diacerein is a highly effective pharmacologic agent in alleviating oxidative damage in PUUO model rats.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahmut Yüksel, Çağdaş Erdoğan, H. Köseoğlu, Salim Neselioglu, Kerem Kenarli, Ahmet Akbay, Meryem D. Göktaş, Çağdaş Kalkan, M. Hamamcı, Mustafa M. Dölek, Yavuz Cagir, Ö. Erel
The objective of this research was to explore the link between Helicobacter pylori (H. pylori) infection and alterations in ischemia modified albumin (IMA), thiol, and disulfide levels, with a focus on their potential clinical implications. We carried out a cross-sectional study, enrolling 153 patients who underwent upper gastrointestinal endoscopy between March and July 2023. Biopsies were obtained from the stomach antrum to diagnose H. pylori. Biochemical parameters, including IMA, thiol, and disulfide, were measured in fasting blood samples. A statistical analysis, including receiver operating characteristic curve analysis, was performed to assess the diagnostic potential of these biomarkers. In this study, a total of 153 patients were included, of whom 99 tested positive for H. pylori and 54 tested negative. The H. pylori-positive group exhibited significantly higher levels of disulfide, disulfide/native thiol ratio, disulfide/total thiol ratio, and IMA compared to the H. pylori-negative group (p≤0.05 for all parameters). In contrast, the native thiol/total thiol ratio was significantly lower in the H. pylori-positive group (p≤0.05). Our study’s findings of elevated disulfide levels in H. pylori-positive individuals suggest a potential disruption in redox balance associated with H. pylori infection. This study contributes to the understanding of H. pylori’s systemic effects on biochemical markers, offering insights into their diagnostic utility.
{"title":"Unveiling the link: Helicobacter pylori infection and impact on ischemia modified albumin, thiol, and disulfide levels","authors":"Mahmut Yüksel, Çağdaş Erdoğan, H. Köseoğlu, Salim Neselioglu, Kerem Kenarli, Ahmet Akbay, Meryem D. Göktaş, Çağdaş Kalkan, M. Hamamcı, Mustafa M. Dölek, Yavuz Cagir, Ö. Erel","doi":"10.1515/tjb-2024-0016","DOIUrl":"https://doi.org/10.1515/tjb-2024-0016","url":null,"abstract":"\u0000 \u0000 \u0000 The objective of this research was to explore the link between Helicobacter pylori (H. pylori) infection and alterations in ischemia modified albumin (IMA), thiol, and disulfide levels, with a focus on their potential clinical implications.\u0000 \u0000 \u0000 \u0000 We carried out a cross-sectional study, enrolling 153 patients who underwent upper gastrointestinal endoscopy between March and July 2023. Biopsies were obtained from the stomach antrum to diagnose H. pylori. Biochemical parameters, including IMA, thiol, and disulfide, were measured in fasting blood samples. A statistical analysis, including receiver operating characteristic curve analysis, was performed to assess the diagnostic potential of these biomarkers.\u0000 \u0000 \u0000 \u0000 In this study, a total of 153 patients were included, of whom 99 tested positive for H. pylori and 54 tested negative. The H. pylori-positive group exhibited significantly higher levels of disulfide, disulfide/native thiol ratio, disulfide/total thiol ratio, and IMA compared to the H. pylori-negative group (p≤0.05 for all parameters). In contrast, the native thiol/total thiol ratio was significantly lower in the H. pylori-positive group (p≤0.05).\u0000 \u0000 \u0000 \u0000 Our study’s findings of elevated disulfide levels in H. pylori-positive individuals suggest a potential disruption in redox balance associated with H. pylori infection. This study contributes to the understanding of H. pylori’s systemic effects on biochemical markers, offering insights into their diagnostic utility.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"29 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic retinopathy (DR) is a retinal disease that arises from impaired glucose tolerance and leads to retinal microvascular leakages. Recent studies have indicated that DR pathogenesis is linked to dysfunctional retinal pigment epithelial (RPE) cells. Investigating the potential interplay between endothelial cells (ECs) and RPE cells by treating ECs with high glucose (HG) and evaluating the function of cytokines released from ECs on the growth of RPE cells. The results revealed that high glucose-stimulated Human Umbilical Vein Endothelial Cells (HUVECs) activated the NF-κB signaling pathway, increased intracellular levels of reactive oxygen species (ROS) and expression of caspase 3 while also elevating HUVECs delivery of cytokines such as VEGF, TNF-α, IL-6, and IL-1β. As a result of our study, cytokines released from HG-treated HUVECs impede the growth of ARPE-19 in vitro, highlighting the importance of functional ECs for exploring the underlying mechanisms of vascular-associated retinal dysfunction. Inflammatory factors secreted from endothelial cells induced by high glucose impair human retinal pigment epithelial cells.
{"title":"Inflammatory factors secreted from endothelial cells induced by high glucose impair human retinal pigment epithelial cells","authors":"Hui Yao, Tingjun Li, Jing Zhang","doi":"10.1515/tjb-2023-0156","DOIUrl":"https://doi.org/10.1515/tjb-2023-0156","url":null,"abstract":"\u0000 \u0000 \u0000 Diabetic retinopathy (DR) is a retinal disease that arises from impaired glucose tolerance and leads to retinal microvascular leakages. Recent studies have indicated that DR pathogenesis is linked to dysfunctional retinal pigment epithelial (RPE) cells.\u0000 \u0000 \u0000 \u0000 Investigating the potential interplay between endothelial cells (ECs) and RPE cells by treating ECs with high glucose (HG) and evaluating the function of cytokines released from ECs on the growth of RPE cells.\u0000 \u0000 \u0000 \u0000 The results revealed that high glucose-stimulated Human Umbilical Vein Endothelial Cells (HUVECs) activated the NF-κB signaling pathway, increased intracellular levels of reactive oxygen species (ROS) and expression of caspase 3 while also elevating HUVECs delivery of cytokines such as VEGF, TNF-α, IL-6, and IL-1β.\u0000 \u0000 \u0000 \u0000 As a result of our study, cytokines released from HG-treated HUVECs impede the growth of ARPE-19 in vitro, highlighting the importance of functional ECs for exploring the underlying mechanisms of vascular-associated retinal dysfunction. Inflammatory factors secreted from endothelial cells induced by high glucose impair human retinal pigment epithelial cells.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140210215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZRF1 (Zuotin-related factor 1) is a versatile protein engaged in protein folding, gene regulation, cellular differentiation, DNA damage response, and immune system and cancer development regulation. This study investigates the role of ZRF1 in monocyte-to-macrophage transformation, and its effects on cell proliferation and the cell cycle. We generated ZRF1-depleted THP-1 cells and induced macrophage differentiation using phorbol 12-myristate 13-acetate (PMA). Differentiation was assessed via microscopy and flow cytometry, while cell proliferation was quantified with the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] (MTS) assay, and the cell cycle was analyzed through flow cytometry using propidium iodide staining. ZRF1-depleted THP-1 cells exhibited notable morphological changes. Flow cytometry post-PMA treatment indicated these cells were smaller and less granular than controls. Proliferation rates of ZRF1-depleted monocytes and macrophages were significantly higher than controls, particularly over longer durations. Cell cycle analysis showed ZRF1 depletion notably affected the G0-G1 phase, highlighting its significant role in macrophage differentiation. The findings provide important insights into ZRF1’s role in monocyte-to-macrophage differentiation and its impact on cell proliferation and the cell cycle. This research not only supports existing knowledge about ZRF1 but also enhances our understanding of its multifaceted roles in cellular processes.
{"title":"Elucidating the role of ZRF1 in monocyte-to-macrophage differentiation, cell proliferation and cell cycle in THP-1 cells","authors":"Aysegul Kaymak Ozdemir, Mahinur Basci","doi":"10.1515/tjb-2024-0015","DOIUrl":"https://doi.org/10.1515/tjb-2024-0015","url":null,"abstract":"\u0000 \u0000 \u0000 ZRF1 (Zuotin-related factor 1) is a versatile protein engaged in protein folding, gene regulation, cellular differentiation, DNA damage response, and immune system and cancer development regulation. This study investigates the role of ZRF1 in monocyte-to-macrophage transformation, and its effects on cell proliferation and the cell cycle.\u0000 \u0000 \u0000 \u0000 We generated ZRF1-depleted THP-1 cells and induced macrophage differentiation using phorbol 12-myristate 13-acetate (PMA). Differentiation was assessed via microscopy and flow cytometry, while cell proliferation was quantified with the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] (MTS) assay, and the cell cycle was analyzed through flow cytometry using propidium iodide staining.\u0000 \u0000 \u0000 \u0000 ZRF1-depleted THP-1 cells exhibited notable morphological changes. Flow cytometry post-PMA treatment indicated these cells were smaller and less granular than controls. Proliferation rates of ZRF1-depleted monocytes and macrophages were significantly higher than controls, particularly over longer durations. Cell cycle analysis showed ZRF1 depletion notably affected the G0-G1 phase, highlighting its significant role in macrophage differentiation.\u0000 \u0000 \u0000 \u0000 The findings provide important insights into ZRF1’s role in monocyte-to-macrophage differentiation and its impact on cell proliferation and the cell cycle. This research not only supports existing knowledge about ZRF1 but also enhances our understanding of its multifaceted roles in cellular processes.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"166 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140222900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In hepatocellular carcinoma (HCC), tumorigenesis, hypoxia and reactive oxygen species (ROS) play a crucial role in altering the tumor microenvironment (TME). Until now, the time-dependent alteration of hypoxia-inducible factor-1α (HIF-1α), the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) under hypoxic conditions in HCC were not clear. Consequently, our main target was to investigate the role of GPx and GR status in HCC cell line (HepG2) under hypoxic conditions. HIF-1α protein levels in cell lysates were determined by ELISA assay and protein expressions were identified using western blot. GPx and GR activity levels of the cell lysates were measured spectrophotometrically. HIF-1α protein levels were determined under normoxic and hypoxic conditions (p<0.001). Also, HIF-1α protein levels and expressions were observed under time-dependent hypoxic conditions, the HIF-1α protein level is found to be reached its peak point at 4 h in the HepG2 cell line. We also have detected decreased activity levels of GPx and increased GR activity levels under hypoxia for 4 h (p<0.001). More than 4 h of exposure to hypoxic environment reducted the HIF-1α levels in HCC cells. According to the results, we suggest the ideal exposure time to hypoxic conditions as 4 h for the HepG2 cell line. In addition, hypoxia also stimulated the activity levels of GPx and GR. Our results suggest that the activity levels of GPx and/or GR enzymes may be therapeutic targets in the hypoxia-dependent HCC tumorigenesis process.
{"title":"GSH-related enzyme activity and tumor relation: glutathione peroxidase and glutathione reductase status under hypoxia in HepG2 cells","authors":"O. Ozensoy Guler, Elif Ercan, T. Uysal","doi":"10.1515/tjb-2023-0044","DOIUrl":"https://doi.org/10.1515/tjb-2023-0044","url":null,"abstract":"\u0000 \u0000 \u0000 In hepatocellular carcinoma (HCC), tumorigenesis, hypoxia and reactive oxygen species (ROS) play a crucial role in altering the tumor microenvironment (TME). Until now, the time-dependent alteration of hypoxia-inducible factor-1α (HIF-1α), the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) under hypoxic conditions in HCC were not clear. Consequently, our main target was to investigate the role of GPx and GR status in HCC cell line (HepG2) under hypoxic conditions.\u0000 \u0000 \u0000 \u0000 HIF-1α protein levels in cell lysates were determined by ELISA assay and protein expressions were identified using western blot. GPx and GR activity levels of the cell lysates were measured spectrophotometrically.\u0000 \u0000 \u0000 \u0000 HIF-1α protein levels were determined under normoxic and hypoxic conditions (p<0.001). Also, HIF-1α protein levels and expressions were observed under time-dependent hypoxic conditions, the HIF-1α protein level is found to be reached its peak point at 4 h in the HepG2 cell line. We also have detected decreased activity levels of GPx and increased GR activity levels under hypoxia for 4 h (p<0.001).\u0000 \u0000 \u0000 \u0000 More than 4 h of exposure to hypoxic environment reducted the HIF-1α levels in HCC cells. According to the results, we suggest the ideal exposure time to hypoxic conditions as 4 h for the HepG2 cell line. In addition, hypoxia also stimulated the activity levels of GPx and GR. Our results suggest that the activity levels of GPx and/or GR enzymes may be therapeutic targets in the hypoxia-dependent HCC tumorigenesis process.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140245266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To study the relationship between DNA methylation and tumour development and provide experimental evidence for the personalized diagnosis and treatment of hepatic carcinoma. The DNA of hepatic carcinoma tissue (Ca group) and adjacent normal tissue (T group) were extracted using the phenol-chloroform method and then treated with bisulfite. Twenty-five genes including 45 subtypes were amplified by PCR. The PCR products were sequenced via the Illumina 450k methylation array assay. The changes of methylated DNA performance were analysed through principal component analysis (PCA). Cluster analysis was used to evaluate the classification of methylated DNA regions. Haplotype abundance variation was tested for methylation differences. Statistical analysis was performed using the chi-square (χ2) test or Fisher’s exact test. Sequencing discoveries indicated CG-type methylation pervading all amplicons. However, CHG-type and CHH-type methylations were confined to only four amplicons (or nine subtypes). The methylation ratios of three specific amplicons (DAB2IP, PRDM14-1, Rab31-1) out of 45 amplicon subtypes in the Ca group significantly increased (over 10 %) compared to the T group (p<0.05). Nineteen amplicons demonstrated minor distinction (methylation pattern variations between 1 and 10 %), with the remaining 23 amplicons showing only minimal disparities (under 1 %). PCA and cluster analysis unveiled a marked difference in methylation levels between cancerous and healthy tissues (p<0.05). The changes in haplotypes and methylation sites could serve as a biomarker for the clinical diagnosis of hepatic carcinoma. Methylation patterns might play an important role in the occurrence and development of hepatic carcinoma.
{"title":"Revealing distinct DNA methylation patterns in hepatic carcinoma through high-throughput sequencing","authors":"Guangmou Zhang, Kefeng Zhang, Meng Yuan, Yichen Li, Jiahui Li, Zhiqing Yuan","doi":"10.1515/tjb-2023-0151","DOIUrl":"https://doi.org/10.1515/tjb-2023-0151","url":null,"abstract":"\u0000 \u0000 \u0000 To study the relationship between DNA methylation and tumour development and provide experimental evidence for the personalized diagnosis and treatment of hepatic carcinoma.\u0000 \u0000 \u0000 \u0000 The DNA of hepatic carcinoma tissue (Ca group) and adjacent normal tissue (T group) were extracted using the phenol-chloroform method and then treated with bisulfite. Twenty-five genes including 45 subtypes were amplified by PCR. The PCR products were sequenced via the Illumina 450k methylation array assay. The changes of methylated DNA performance were analysed through principal component analysis (PCA). Cluster analysis was used to evaluate the classification of methylated DNA regions. Haplotype abundance variation was tested for methylation differences. Statistical analysis was performed using the chi-square (χ2) test or Fisher’s exact test.\u0000 \u0000 \u0000 \u0000 Sequencing discoveries indicated CG-type methylation pervading all amplicons. However, CHG-type and CHH-type methylations were confined to only four amplicons (or nine subtypes). The methylation ratios of three specific amplicons (DAB2IP, PRDM14-1, Rab31-1) out of 45 amplicon subtypes in the Ca group significantly increased (over 10 %) compared to the T group (p<0.05). Nineteen amplicons demonstrated minor distinction (methylation pattern variations between 1 and 10 %), with the remaining 23 amplicons showing only minimal disparities (under 1 %). PCA and cluster analysis unveiled a marked difference in methylation levels between cancerous and healthy tissues (p<0.05).\u0000 \u0000 \u0000 \u0000 The changes in haplotypes and methylation sites could serve as a biomarker for the clinical diagnosis of hepatic carcinoma. Methylation patterns might play an important role in the occurrence and development of hepatic carcinoma.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"351 1‐3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. K. Açikgözoğlu, Ş. Pala, R. Atılgan, Nevin Ilhan, N. Ilhan
Investigation of angiopoietin-like protein-4 (ANGPTL-4) and vascular endothelial growth factor A (VEGF-A) levels as a biochemical marker in gestational hypertension (GH) and preeclampsia (PE), which are known to have important roles in the maintenance of angiogenesis and endothelial functions. Total of 90 patients included in this case-control study. Group 1 (G1) (n=30)=patients with healthy pregnancy between 37 and 41 weeks, G2 (n=30)=patients diagnosed with gestational hypertension between 20 and 37 weeks, G3 (n=30)=patients diagnosed with preeclampsia between 20 and 37 weeks. The sera obtained from the patients were stored at −80 °C until they were studied. Demographic parameters, systolic, diastolic and mean arterial blood pressure were recorded. VEGF-A and ANGPTL-4 levels were studied with enzyme-linked immunosorbent assay (ELISA) kit. The mean age was similar in both groups. The number of primigravida pregnant was higher in G2 and G3 than in G1. Gestational week was more advanced in G1 compared to G2 and G3. While ANGPTL-4 and VEGF-A levels were similar between G2 and G3, they were significantly higher in both groups compared to G1. We showed that ANGPTL-4 and VEGF-A levels were elevated in maternal serum in GH and PE cases. Increased maternal serum ANGPTL-4 levels may be a biomarker that can be used in the early diagnosis of PE.
{"title":"High serum angiopoietin-like protein-4 levels are associated with gestational hypertension and preeclampsia: a case-control study","authors":"M. K. Açikgözoğlu, Ş. Pala, R. Atılgan, Nevin Ilhan, N. Ilhan","doi":"10.1515/tjb-2023-0087","DOIUrl":"https://doi.org/10.1515/tjb-2023-0087","url":null,"abstract":"\u0000 \u0000 \u0000 Investigation of angiopoietin-like protein-4 (ANGPTL-4) and vascular endothelial growth factor A (VEGF-A) levels as a biochemical marker in gestational hypertension (GH) and preeclampsia (PE), which are known to have important roles in the maintenance of angiogenesis and endothelial functions.\u0000 \u0000 \u0000 \u0000 Total of 90 patients included in this case-control study. Group 1 (G1) (n=30)=patients with healthy pregnancy between 37 and 41 weeks, G2 (n=30)=patients diagnosed with gestational hypertension between 20 and 37 weeks, G3 (n=30)=patients diagnosed with preeclampsia between 20 and 37 weeks. The sera obtained from the patients were stored at −80 °C until they were studied. Demographic parameters, systolic, diastolic and mean arterial blood pressure were recorded. VEGF-A and ANGPTL-4 levels were studied with enzyme-linked immunosorbent assay (ELISA) kit.\u0000 \u0000 \u0000 \u0000 The mean age was similar in both groups. The number of primigravida pregnant was higher in G2 and G3 than in G1. Gestational week was more advanced in G1 compared to G2 and G3. While ANGPTL-4 and VEGF-A levels were similar between G2 and G3, they were significantly higher in both groups compared to G1.\u0000 \u0000 \u0000 \u0000 We showed that ANGPTL-4 and VEGF-A levels were elevated in maternal serum in GH and PE cases. Increased maternal serum ANGPTL-4 levels may be a biomarker that can be used in the early diagnosis of PE.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140252357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ultra-rare DES c.1289-2A>G mutation, resulting in a 48-base pair insertion in the Desmin tail domain, is associated with late-onset MFM1 (myofibrillar myopathy-1; OMIM number; 601419) and exhibits distinctive pathological features. Despite sustained expression and cytoskeletal integrity, muscle biopsies reveal dystrophic characteristics through an unidentified mechanism. A deeper understanding of the molecular mechanisms underlying Desmin-related MFM1 could enhance our perspective and comprehension of the disease’s pathophysiology. In this study, we aimed to investigate the pathological phenotype by assessing the myogenic potency of MyoD-induced patient-derived fibroblasts. Following the immortalization and myoconversion of unaffected and patient-derived fibroblast cells, we analyzed the myogenic potency of the mutant and control groups on day 5 post-differentiation. This analysis involved staining cells with MF20 antibody and DAPI after MyoD induction. Employing six parameters to quantify extra nuclei and myotube properties, we unveil impaired myogenic differentiation in c.1289-2A>G mutant cells, as evidenced by a compromised fusion index and distinctive myogenic features. In summary, our preliminary findings indicate phenotypic abnormalities and suggest an association between the DES c.1289-2A>G mutation and delayed maturation and MFM in affected individuals. Our results indicate a significant involvement of Desmin in the myogenic maturation of muscle cells. Further investigation is required to understand the changes in the transcriptome during the myoconversion of patient-derived fibroblasts.
{"title":"Assessment of myogenic potency in patient-derived fibroblasts with c.1289-2A>G Desmin mutation","authors":"Nilüfer Düz, Şeyda Ünsal, Sevim Eerdem-Özdamar, Pervin Dinçer","doi":"10.1515/tjb-2023-0264","DOIUrl":"https://doi.org/10.1515/tjb-2023-0264","url":null,"abstract":"\u0000 \u0000 \u0000 The ultra-rare DES c.1289-2A>G mutation, resulting in a 48-base pair insertion in the Desmin tail domain, is associated with late-onset MFM1 (myofibrillar myopathy-1; OMIM number; 601419) and exhibits distinctive pathological features. Despite sustained expression and cytoskeletal integrity, muscle biopsies reveal dystrophic characteristics through an unidentified mechanism. A deeper understanding of the molecular mechanisms underlying Desmin-related MFM1 could enhance our perspective and comprehension of the disease’s pathophysiology. In this study, we aimed to investigate the pathological phenotype by assessing the myogenic potency of MyoD-induced patient-derived fibroblasts.\u0000 \u0000 \u0000 \u0000 Following the immortalization and myoconversion of unaffected and patient-derived fibroblast cells, we analyzed the myogenic potency of the mutant and control groups on day 5 post-differentiation. This analysis involved staining cells with MF20 antibody and DAPI after MyoD induction.\u0000 \u0000 \u0000 \u0000 Employing six parameters to quantify extra nuclei and myotube properties, we unveil impaired myogenic differentiation in c.1289-2A>G mutant cells, as evidenced by a compromised fusion index and distinctive myogenic features. In summary, our preliminary findings indicate phenotypic abnormalities and suggest an association between the DES c.1289-2A>G mutation and delayed maturation and MFM in affected individuals.\u0000 \u0000 \u0000 \u0000 Our results indicate a significant involvement of Desmin in the myogenic maturation of muscle cells. Further investigation is required to understand the changes in the transcriptome during the myoconversion of patient-derived fibroblasts.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"8 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140261905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nucleocytoplasmic transport is one of the key features in regulation of cellular physiology. Developing a better understanding of the molecular mechanism underlying the nucleocytoplasmic shuttling of proteins can broaden our perspective and understanding on the elaborate sorting mechanisms within cells. Desmin is a muscle specific intermediate filament with amphiphilic properties and has interactions with the components of the nuclear pore complex which facilitates the transport between the cytoplasm and nucleus. The study aims to develop a better understanding of the amphiphilic nature of desmin and its relation to nucleocytoplasmic transport. We conducted a proteomic analysis of desmin-immunoprecipitates to identify the nuclear partners of desmin. Additionally, we analysed the amphiphilic nature of desmin using a hydrophobicity assay to determine if it can undergo conformational changes to adapt to a hydrophobic environment. Using proteomic and in silico analysis we demonstrated that desmin interacts with several nups. The hydrophobicity assay results showed that desmin can increase its surface hydrophobicity in a hydrophobic environment. Our findings suggest that desmin has the ability to undergo conformational changes under favourable conditions and possibly can be transported through nucleus via direct interaction with nups. Further analysis is required to understand the functional implications of this conformational change in vivo. Data are available via ProteomeXchange with identifier PXD047121.
{"title":"Desmin’s conformational modulation by hydrophobicity","authors":"Ecem Kural Mangıt, Orkun Cevheroğlu, Pervin Dinçer","doi":"10.1515/tjb-2023-0220","DOIUrl":"https://doi.org/10.1515/tjb-2023-0220","url":null,"abstract":"\u0000 \u0000 \u0000 Nucleocytoplasmic transport is one of the key features in regulation of cellular physiology. Developing a better understanding of the molecular mechanism underlying the nucleocytoplasmic shuttling of proteins can broaden our perspective and understanding on the elaborate sorting mechanisms within cells. Desmin is a muscle specific intermediate filament with amphiphilic properties and has interactions with the components of the nuclear pore complex which facilitates the transport between the cytoplasm and nucleus. The study aims to develop a better understanding of the amphiphilic nature of desmin and its relation to nucleocytoplasmic transport.\u0000 \u0000 \u0000 \u0000 We conducted a proteomic analysis of desmin-immunoprecipitates to identify the nuclear partners of desmin. Additionally, we analysed the amphiphilic nature of desmin using a hydrophobicity assay to determine if it can undergo conformational changes to adapt to a hydrophobic environment.\u0000 \u0000 \u0000 \u0000 Using proteomic and in silico analysis we demonstrated that desmin interacts with several nups. The hydrophobicity assay results showed that desmin can increase its surface hydrophobicity in a hydrophobic environment.\u0000 \u0000 \u0000 \u0000 Our findings suggest that desmin has the ability to undergo conformational changes under favourable conditions and possibly can be transported through nucleus via direct interaction with nups. Further analysis is required to understand the functional implications of this conformational change in vivo. Data are available via ProteomeXchange with identifier PXD047121.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"100 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Bolat, Vildan Fidancı, Deniz Elçik, Özdem Kavraz Tomar, S. N. Murat, Murat Duranay, Doğan Yücel
The cardiovascular mortality risk is greatly increased in patients with chronic kidney disease (CKD), especially in dialysis patients, due to atherosclerosis. Platelet activating factor acetylhydrolase (PAF-AH) is an enzyme that hydrolyzes platelet activating factor (PAF). Valvular calcifications and PAF-AH are associated with atherosclerosis. However, little is known about the status of PAF-AH activity and valvular calcification in dialysis patients. Therefore, the aim of this study was to investigate the status of these parameters in CKD patients. This study included 92 chronic renal failure (CRF) (dialysis group), and 86 CKD patients (non-dialysis group). Echocardiography was performed to assess valvular calcification. There was no significant difference between the dialysis and CKD groups in terms of PAF-AH activities. However, when comparisons were stratified according to the presence of valve calcification, higher PAF-AH activity and N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels were evident in patients with calcification compared to those without. Additionally, the CRF group also exhibited elevated PAF-AH and NT-proBNP levels. While elevated NT-proBNP persisted in the CKD group, in contrast, changes in PAF-AH were not significant. The results of this study suggest that high PAF-AH and NT-proBNP levels are associated with valvular calcification in dialysis patients. Both biomarkers may be used as a risk factor for calcification. Furthermore, inhibition of PAF-AH activity may be a treatment target to reduce calcification.
{"title":"Platelet activating factor acetylhydrolase is associated with cardiac valvular calcification in dialysis patients","authors":"S. Bolat, Vildan Fidancı, Deniz Elçik, Özdem Kavraz Tomar, S. N. Murat, Murat Duranay, Doğan Yücel","doi":"10.1515/tjb-2023-0263","DOIUrl":"https://doi.org/10.1515/tjb-2023-0263","url":null,"abstract":"\u0000 \u0000 \u0000 The cardiovascular mortality risk is greatly increased in patients with chronic kidney disease (CKD), especially in dialysis patients, due to atherosclerosis. Platelet activating factor acetylhydrolase (PAF-AH) is an enzyme that hydrolyzes platelet activating factor (PAF). Valvular calcifications and PAF-AH are associated with atherosclerosis. However, little is known about the status of PAF-AH activity and valvular calcification in dialysis patients. Therefore, the aim of this study was to investigate the status of these parameters in CKD patients.\u0000 \u0000 \u0000 \u0000 This study included 92 chronic renal failure (CRF) (dialysis group), and 86 CKD patients (non-dialysis group). Echocardiography was performed to assess valvular calcification.\u0000 \u0000 \u0000 \u0000 There was no significant difference between the dialysis and CKD groups in terms of PAF-AH activities. However, when comparisons were stratified according to the presence of valve calcification, higher PAF-AH activity and N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels were evident in patients with calcification compared to those without. Additionally, the CRF group also exhibited elevated PAF-AH and NT-proBNP levels. While elevated NT-proBNP persisted in the CKD group, in contrast, changes in PAF-AH were not significant.\u0000 \u0000 \u0000 \u0000 The results of this study suggest that high PAF-AH and NT-proBNP levels are associated with valvular calcification in dialysis patients. Both biomarkers may be used as a risk factor for calcification. Furthermore, inhibition of PAF-AH activity may be a treatment target to reduce calcification.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140089102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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