Toll-like receptor (TLR) mediated inflammatory status plays an important role in development and progression of hepatocellular carcinoma (HCC). Toll-interacting protein (TOLLIP) has an inhibitory effect on TLR-mediated inflammatory signalling and expression profile of TOLLIP varies between malignancies including HCC. Cholinergic anti-inflammatory pathway (CAP) is an endogenous mechanism that controls inflammatory status via α7nicotinic acetylcholine receptors (α7nAChR). This study aims to investigate the effect of CAP-acting agent choline on TOLLIP and its related TLR-mediated inflammatory response in HCC cells with distinct differentiation stages. The expression patterns of α7nAChR, TLR2/4, TOLLIP, IL6,NFkB genes were evaluated by RT-PCR and ELISA in the presence of choline, along with the real-time cell proliferation and migration in HEP3B and SNU449 HCC cell lines. The interaction between choline and TOLLIP assessed by using in-silico analyses. Choline downregulated TOLLIP in Hep3B and SNU449 cells. However, the expressions of α7nAChR, NF-κB, IL-6, TLR2 and TLR4 showed a decreased pattern in well differentiated HEP3B cells, while an increased pattern in poorly differentiated SNU449 cells. Choline might exert differential effects in TLR2/4-dependent signalling based on the differentiation stages of the HCC cells, suggesting its potential therapeutic effects in earlier stages of HCC which might be result of its partial modulation of TOLLIP.
{"title":"Differential effects of choline on TLR2/4 mediated signaling through possible regulation of toll-interacting protein in hepatocellular carcinoma cell lines","authors":"Elif Baris, Ayse Banu Demir","doi":"10.1515/tjb-2023-0282","DOIUrl":"https://doi.org/10.1515/tjb-2023-0282","url":null,"abstract":"\u0000 \u0000 \u0000 Toll-like receptor (TLR) mediated inflammatory status plays an important role in development and progression of hepatocellular carcinoma (HCC). Toll-interacting protein (TOLLIP) has an inhibitory effect on TLR-mediated inflammatory signalling and expression profile of TOLLIP varies between malignancies including HCC. Cholinergic anti-inflammatory pathway (CAP) is an endogenous mechanism that controls inflammatory status via α7nicotinic acetylcholine receptors (α7nAChR). This study aims to investigate the effect of CAP-acting agent choline on TOLLIP and its related TLR-mediated inflammatory response in HCC cells with distinct differentiation stages.\u0000 \u0000 \u0000 \u0000 The expression patterns of α7nAChR, TLR2/4, TOLLIP, IL6,NFkB genes were evaluated by RT-PCR and ELISA in the presence of choline, along with the real-time cell proliferation and migration in HEP3B and SNU449 HCC cell lines. The interaction between choline and TOLLIP assessed by using in-silico analyses.\u0000 \u0000 \u0000 \u0000 Choline downregulated TOLLIP in Hep3B and SNU449 cells. However, the expressions of α7nAChR, NF-κB, IL-6, TLR2 and TLR4 showed a decreased pattern in well differentiated HEP3B cells, while an increased pattern in poorly differentiated SNU449 cells.\u0000 \u0000 \u0000 \u0000 Choline might exert differential effects in TLR2/4-dependent signalling based on the differentiation stages of the HCC cells, suggesting its potential therapeutic effects in earlier stages of HCC which might be result of its partial modulation of TOLLIP.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141098276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Emin, D. Cibrev, Coskun Kerala, D. Petrovska-Cvetkovska, Valdrina Ajeti, H. Ampova, I. Kostovska, K. Tosheska-Trajkovska
The aim of this study was to describe the effectiveness of the vaccines (Tozinameran and Sputnik V), administered on a convenience sample of healthcare workers, and also to describe the relationship between the levels of neutralizing antibodies (NAbs) and the type of vaccine used, as well as their association with incident cases during follow-up. The study included 262 participants, who underwent vaccination during the period from September 2021 until August 2022. For determining the levels of NAbs we used the CLIA based method, and all the samples were processed with the SNIBE Maglumi 800 analyzer. The patients were observed for one year for occurrence of incident infection. The participants with prior SARS-CoV-2 positivity showed substantially higher titer of NAbs (8.86 vs. 0.94, p<0.001). The participants in the Gam-COVID-Vac group had median levels of NAbs of 1.57 (IQR 0.42–5.73), while they in the Tozinameran group showed substantially higher levels of 2.37 (IQR 0.9–6.27). The incident cases after immunization had substantially lower median values of NAbs when compared to the rest (0.48 vs. 3.97, p<0.001), and the interval between the second dose and the serological measurements were similar. The current study showed that the tested vaccines demonstrated vaccine effectiveness of over 50 % during the first year after the vaccination in a sample of health care workers. Although health care workers remain separate population group, when compared to the rest, the results could be extrapolated to populations with similar age and immune experience.
{"title":"Effectiveness after immunization with BNT162b2 and Gam-COVID-Vac for SARS-CoV-2 and neutralizing antibody titers in health care workers","authors":"M. Emin, D. Cibrev, Coskun Kerala, D. Petrovska-Cvetkovska, Valdrina Ajeti, H. Ampova, I. Kostovska, K. Tosheska-Trajkovska","doi":"10.1515/tjb-2023-0213","DOIUrl":"https://doi.org/10.1515/tjb-2023-0213","url":null,"abstract":"\u0000 \u0000 \u0000 The aim of this study was to describe the effectiveness of the vaccines (Tozinameran and Sputnik V), administered on a convenience sample of healthcare workers, and also to describe the relationship between the levels of neutralizing antibodies (NAbs) and the type of vaccine used, as well as their association with incident cases during follow-up.\u0000 \u0000 \u0000 \u0000 The study included 262 participants, who underwent vaccination during the period from September 2021 until August 2022. For determining the levels of NAbs we used the CLIA based method, and all the samples were processed with the SNIBE Maglumi 800 analyzer. The patients were observed for one year for occurrence of incident infection.\u0000 \u0000 \u0000 \u0000 The participants with prior SARS-CoV-2 positivity showed substantially higher titer of NAbs (8.86 vs. 0.94, p<0.001). The participants in the Gam-COVID-Vac group had median levels of NAbs of 1.57 (IQR 0.42–5.73), while they in the Tozinameran group showed substantially higher levels of 2.37 (IQR 0.9–6.27). The incident cases after immunization had substantially lower median values of NAbs when compared to the rest (0.48 vs. 3.97, p<0.001), and the interval between the second dose and the serological measurements were similar.\u0000 \u0000 \u0000 \u0000 The current study showed that the tested vaccines demonstrated vaccine effectiveness of over 50 % during the first year after the vaccination in a sample of health care workers. Although health care workers remain separate population group, when compared to the rest, the results could be extrapolated to populations with similar age and immune experience.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"64 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141101928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Yalçındağ, Fevzi Nuri Aydın, Özben Özden Işıklar, Fatmagül Can, Murat Karademir, Ersen Uzunal, A. O. Akyurek, Doğan Yücel, M. A. Serdar
Clinical Biochemistry Laboratories (CBL) are the most frequently utilized laboratory group in healthcare, and their significance in patient care is indisputable. This study investigated the frequency and pattern of test utilization rate in CBL at two large hospitals’ outpatient and inpatient clinics. A total of 43,732,428 CBL tests, including clinical chemistry, immunoassay, coagulation, specific proteins, CBC, and urinalysis, were conducted for 12,182,382 patients across two large hospitals in different settings between 2018 and 2022. These tests were analyzed alongside patient admissions data, with a focus on the distribution across various clinics. 94 and 93 % of those admitted to Hospitals 1 and 2 were outpatients. They had applied to CBL laboratories for 27.1–30.3 % of outpatients and 81.2–88.7 % of inpatients for at least one test. When analyzing the rates at which laboratory tests were requested for outpatients, it was found that emergency departments had the highest test-requesting rates, ranging from 19.99 to 45.36 %. This was followed by internal medicine clinics, with rates ranging from 13.77 to 14.8 %, and inpatient intensive care units, with rates between 24.31 and 30.14 %. Outpatients had 10–11 test requests for each patient and 16–31 for inpatients. The most frequently requested laboratory tests were CBC, glucose, creatinine, urea, AST and ALT in two hospitals. Despite significant variations in location, structure, medical staff, and patient demographics, approximately one-third of outpatients and 85 % of inpatients at these hospitals undergo testing in CBL. CBLs are essential for screening, diagnosis, prognosis, and healthcare treatment.
{"title":"Frequency and pattern of test utilization rate in clinical biochemistry laboratory: two different large hospital examples","authors":"Ali Yalçındağ, Fevzi Nuri Aydın, Özben Özden Işıklar, Fatmagül Can, Murat Karademir, Ersen Uzunal, A. O. Akyurek, Doğan Yücel, M. A. Serdar","doi":"10.1515/tjb-2023-0099","DOIUrl":"https://doi.org/10.1515/tjb-2023-0099","url":null,"abstract":"\u0000 \u0000 \u0000 Clinical Biochemistry Laboratories (CBL) are the most frequently utilized laboratory group in healthcare, and their significance in patient care is indisputable. This study investigated the frequency and pattern of test utilization rate in CBL at two large hospitals’ outpatient and inpatient clinics.\u0000 \u0000 \u0000 \u0000 A total of 43,732,428 CBL tests, including clinical chemistry, immunoassay, coagulation, specific proteins, CBC, and urinalysis, were conducted for 12,182,382 patients across two large hospitals in different settings between 2018 and 2022. These tests were analyzed alongside patient admissions data, with a focus on the distribution across various clinics.\u0000 \u0000 \u0000 \u0000 94 and 93 % of those admitted to Hospitals 1 and 2 were outpatients. They had applied to CBL laboratories for 27.1–30.3 % of outpatients and 81.2–88.7 % of inpatients for at least one test. When analyzing the rates at which laboratory tests were requested for outpatients, it was found that emergency departments had the highest test-requesting rates, ranging from 19.99 to 45.36 %. This was followed by internal medicine clinics, with rates ranging from 13.77 to 14.8 %, and inpatient intensive care units, with rates between 24.31 and 30.14 %. Outpatients had 10–11 test requests for each patient and 16–31 for inpatients. The most frequently requested laboratory tests were CBC, glucose, creatinine, urea, AST and ALT in two hospitals.\u0000 \u0000 \u0000 \u0000 Despite significant variations in location, structure, medical staff, and patient demographics, approximately one-third of outpatients and 85 % of inpatients at these hospitals undergo testing in CBL. CBLs are essential for screening, diagnosis, prognosis, and healthcare treatment.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"121 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Aydemir, Muammer Yucel, Mehmet Koseoglu, N. N. Ulusu
The elderly population is categorized as a risk group for COVID-19 infection, and dementia is the primary cause of disability in elderly individuals and affects 70 % of the elderly population. In this study, we evaluated the blood and serum biomarkers of deceased dementia patients infected by COVID-19 compared to the survived dementia and non-dementia patients. Laboratory biomarkers of 11 dementia patients infected by COVID-19 have been used for this study. The five patients’ serum biochemistry and blood data were compared with the six patients who died because of COVID-19. Additionally, data from nine patients aged 85–96 infected with COVID-19 without dementia have been used to compare the difference between dementia and non-dementia individuals. D-dimer, C-reactive protein (CRP), glucose, blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate aminotransferase (AST), troponin, procalcitonin, red cell distribution width (RDW), white blood cell (WBC), neutrophil (NEU) and %NEU levels significantly increased in the deceased dementia patients compared to the survived and non-dementia individuals. Calcium (Ca), hematocrit (HCT), red blood cells (RBC), lymphocyte (%LYM), monocyte %MONO, and basophil (%BASO) levels significantly decreased in the deceased dementia patients compared to the survived and non-dementia individuals infected by COVID-19. Serum biochemistry and hematological biomarkers, including D-dimer, CRP, glucose, ALT, AST, BUN, troponin, procalcitonin, RDW, RBC, WBC, NEU, %NEU, Ca, HCT, %LYM, %MONO, and %BASO were significantly altered in deceased dementia patients infected by COVID-19 compared to the survived individuals.
{"title":"Does COVID-19 infection alter serum biochemical and hematological biomarkers in deceased dementia patients?","authors":"D. Aydemir, Muammer Yucel, Mehmet Koseoglu, N. N. Ulusu","doi":"10.1515/tjb-2022-0206","DOIUrl":"https://doi.org/10.1515/tjb-2022-0206","url":null,"abstract":"\u0000 \u0000 \u0000 The elderly population is categorized as a risk group for COVID-19 infection, and dementia is the primary cause of disability in elderly individuals and affects 70 % of the elderly population. In this study, we evaluated the blood and serum biomarkers of deceased dementia patients infected by COVID-19 compared to the survived dementia and non-dementia patients.\u0000 \u0000 \u0000 \u0000 Laboratory biomarkers of 11 dementia patients infected by COVID-19 have been used for this study. The five patients’ serum biochemistry and blood data were compared with the six patients who died because of COVID-19. Additionally, data from nine patients aged 85–96 infected with COVID-19 without dementia have been used to compare the difference between dementia and non-dementia individuals.\u0000 \u0000 \u0000 \u0000 D-dimer, C-reactive protein (CRP), glucose, blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate aminotransferase (AST), troponin, procalcitonin, red cell distribution width (RDW), white blood cell (WBC), neutrophil (NEU) and %NEU levels significantly increased in the deceased dementia patients compared to the survived and non-dementia individuals. Calcium (Ca), hematocrit (HCT), red blood cells (RBC), lymphocyte (%LYM), monocyte %MONO, and basophil (%BASO) levels significantly decreased in the deceased dementia patients compared to the survived and non-dementia individuals infected by COVID-19.\u0000 \u0000 \u0000 \u0000 Serum biochemistry and hematological biomarkers, including D-dimer, CRP, glucose, ALT, AST, BUN, troponin, procalcitonin, RDW, RBC, WBC, NEU, %NEU, Ca, HCT, %LYM, %MONO, and %BASO were significantly altered in deceased dementia patients infected by COVID-19 compared to the survived individuals.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"46 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Serdar, Fatma Demet Arslan, N. Y. Saral, Doğan Yücel
In this study, the aim is to provide a more detailed understanding of vitamin D metabolism by evaluating the correlation between 1,25-dihydroxyvitamin D (1,25(OH)2D) and 25-hydroxyvitamin D (25(OH)D) according to the variations in measurement methods and clinical conditions. We searched PubMed, Embase, and Web of Science for studies reporting correlation results between 1,25(OH)2D and 25(OH)D. We performed a meta-analysis based on the correlation results of 1,25(OH)2D and 25(OH)D in different clinical conditions. We included a total of 63 studies and our laboratory’s results in the meta-analysis. The studies were categorized into high-quality methods group (HQMG), medium-quality methods group (MQMG), and low-quality methods group (LQMG) based on the 25(OH)D and 1,25(OH)2D measurement. In the healthy, renal disease, and other disease groups, the highest correlation values were observed in the studies categorized as HQMG, with values of 0.35 (95 % CI; 0.23–0.48), 0.36 (95 % CI; 0.26–0.42), and 0.36 (95 % CI; 0.22–0.48), respectively. Significant statistical heterogeneity was observed in the healthy, renal disease, and other disease groups, with I2 values of 92.4 , 82.7, and 90.7 %, respectively (p<0.001). Both Funnel plots and the results of Egger’s and Begg’s tests indicated no statistically significant bias across all studies. A significantly low correlation was found between 25(OH)D and 1,25(OH)2D. However, higher correlations were found in the studies categorized as HQMG. Various factors, including methodological inadequacies and disparities, might contribute to this. In the future, with more accurate and reproducible measurements of 1,25(OH)2D, a clearer understanding of vitamin D metabolism will be achieved.
{"title":"Correlation between serum 1,25-dihydroxyvitamin D and 25-hydroxyvitamin D in response to analytical procedures; a systematic review and meta-analysis","authors":"M. A. Serdar, Fatma Demet Arslan, N. Y. Saral, Doğan Yücel","doi":"10.1515/tjb-2023-0258","DOIUrl":"https://doi.org/10.1515/tjb-2023-0258","url":null,"abstract":"\u0000 \u0000 \u0000 In this study, the aim is to provide a more detailed understanding of vitamin D metabolism by evaluating the correlation between 1,25-dihydroxyvitamin D (1,25(OH)2D) and 25-hydroxyvitamin D (25(OH)D) according to the variations in measurement methods and clinical conditions.\u0000 \u0000 \u0000 \u0000 We searched PubMed, Embase, and Web of Science for studies reporting correlation results between 1,25(OH)2D and 25(OH)D. We performed a meta-analysis based on the correlation results of 1,25(OH)2D and 25(OH)D in different clinical conditions. We included a total of 63 studies and our laboratory’s results in the meta-analysis. The studies were categorized into high-quality methods group (HQMG), medium-quality methods group (MQMG), and low-quality methods group (LQMG) based on the 25(OH)D and 1,25(OH)2D measurement.\u0000 \u0000 \u0000 \u0000 In the healthy, renal disease, and other disease groups, the highest correlation values were observed in the studies categorized as HQMG, with values of 0.35 (95 % CI; 0.23–0.48), 0.36 (95 % CI; 0.26–0.42), and 0.36 (95 % CI; 0.22–0.48), respectively. Significant statistical heterogeneity was observed in the healthy, renal disease, and other disease groups, with I2 values of 92.4 , 82.7, and 90.7 %, respectively (p<0.001). Both Funnel plots and the results of Egger’s and Begg’s tests indicated no statistically significant bias across all studies.\u0000 \u0000 \u0000 \u0000 A significantly low correlation was found between 25(OH)D and 1,25(OH)2D. However, higher correlations were found in the studies categorized as HQMG. Various factors, including methodological inadequacies and disparities, might contribute to this. In the future, with more accurate and reproducible measurements of 1,25(OH)2D, a clearer understanding of vitamin D metabolism will be achieved.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"41 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140984007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Demet Kaçaroğlu, Melek Parlak Khalily, Seher Yaylacı
Lung cancer remains a predominant cancer type with high incidence and low survival rates. Key challenges in its treatment include impaired cellular mechanisms, notably resistance to apoptosis and altered immune responses. A critical aspect in this context is the heightened TLR4-mediated signaling, known to promote cell survival, metastasis, and resistance to cell death, particularly impacting immune microenvironment regulation. This study focuses on evaluating the impact of TLR4 signaling activation on potential therapeutic strategies. Our research utilizes Cannabidiol (CBD), a compound already employed in mitigating chemotherapy side effects in lung adenocarcinoma, recognized for its antitumor properties including antiproliferative, antimetastatic, and apoptosis-inducing effects. However, the effectiveness of CBD in lung cancer cells with elevated TLR4 signaling remains uncertain. Our findings reveal that the combination of CBD and TLR4 agonist affects cell viability, proliferation, cell cycle progression, apoptosis, and gene expression related to immune response and extracellular matrix regulation. In lung adenocarcinoma cells with activated TLR4, CBD shows an increased IC50 value, reflecting reduced antiproliferative capacity. Furthermore, its efficacy in arresting the cell cycle and inducing apoptosis is also compromised. The influence on immune response and extracellular matrix regulation is also altered in TLR4-activated cells. These results indicate that TLR4 activation significantly diminishes the antitumor efficacy of CBD. This highlights the importance of considering TLR4 signaling activation in future research on therapeutic agents like CBD for cancer treatment.
{"title":"Influence of TLR4 signaling on Cannabidiol’s antitumor effectiveness in lung adenocarcinoma cells","authors":"Demet Kaçaroğlu, Melek Parlak Khalily, Seher Yaylacı","doi":"10.1515/tjb-2023-0256","DOIUrl":"https://doi.org/10.1515/tjb-2023-0256","url":null,"abstract":"\u0000 \u0000 \u0000 Lung cancer remains a predominant cancer type with high incidence and low survival rates. Key challenges in its treatment include impaired cellular mechanisms, notably resistance to apoptosis and altered immune responses. A critical aspect in this context is the heightened TLR4-mediated signaling, known to promote cell survival, metastasis, and resistance to cell death, particularly impacting immune microenvironment regulation. This study focuses on evaluating the impact of TLR4 signaling activation on potential therapeutic strategies.\u0000 \u0000 \u0000 \u0000 Our research utilizes Cannabidiol (CBD), a compound already employed in mitigating chemotherapy side effects in lung adenocarcinoma, recognized for its antitumor properties including antiproliferative, antimetastatic, and apoptosis-inducing effects. However, the effectiveness of CBD in lung cancer cells with elevated TLR4 signaling remains uncertain.\u0000 \u0000 \u0000 \u0000 Our findings reveal that the combination of CBD and TLR4 agonist affects cell viability, proliferation, cell cycle progression, apoptosis, and gene expression related to immune response and extracellular matrix regulation. In lung adenocarcinoma cells with activated TLR4, CBD shows an increased IC50 value, reflecting reduced antiproliferative capacity. Furthermore, its efficacy in arresting the cell cycle and inducing apoptosis is also compromised. The influence on immune response and extracellular matrix regulation is also altered in TLR4-activated cells.\u0000 \u0000 \u0000 \u0000 These results indicate that TLR4 activation significantly diminishes the antitumor efficacy of CBD. This highlights the importance of considering TLR4 signaling activation in future research on therapeutic agents like CBD for cancer treatment.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141015469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
İbrahim Fettahoğlu, S. Kartalci, H. G. Gözükara Bağ, Ceren Acar
Schizophrenia is a neuropsychiatric disease, and its etiology is not exactly understood. DNA methylation is an important phenomenon that affects the rise of abnormal phenotypes in many cases. Investigation of the association between DNA methylation and schizophrenia is crucial for elucidating the basis of schizophrenia. Previous association studies confirm that the SYN2 gene is a strong candidate gene for schizophrenia. In the current study, the relationship between the methylation status of the SYN2 gene and schizophrenia was investigated. The aim is to obtain crucial results for illuminating the effects of the SYN2 methylation changes in the etiology of schizophrenia. In light of this scientific information, we investigated the methylation status of three different CpG regions in the promoter of the SYN2 gene and compared them in healthy controls and schizophrenia patients. Thirty-three healthy controls and 36 schizophrenia patients were included in this study. Sequencing was performed using the pyrosequencing method to reveal the methylation pattern. As a result of the statistical analysis, it was confirmed that there is a significant relationship between the methylation pattern of the SYN2 gene and schizophrenia. Schizophrenia patients showed more methylation in position 2 and position 3. Additionally, the average methylation ratio is increased in schizophrenia patients. We find an association between the DNA methylation pattern of the SYN2 gene and schizophrenia. These results can help to the understanding of the etiology of schizophrenia. Except for these, DNA methylation changes in the SYN2 gene in people who live in urban and rural areas can be one of the reasons for the different incidences of schizophrenia in these regions.
{"title":"Relationship between methylation pattern of the SYN2 gene and schizophrenia","authors":"İbrahim Fettahoğlu, S. Kartalci, H. G. Gözükara Bağ, Ceren Acar","doi":"10.1515/tjb-2023-0249","DOIUrl":"https://doi.org/10.1515/tjb-2023-0249","url":null,"abstract":"\u0000 \u0000 \u0000 Schizophrenia is a neuropsychiatric disease, and its etiology is not exactly understood. DNA methylation is an important phenomenon that affects the rise of abnormal phenotypes in many cases. Investigation of the association between DNA methylation and schizophrenia is crucial for elucidating the basis of schizophrenia. Previous association studies confirm that the SYN2 gene is a strong candidate gene for schizophrenia. In the current study, the relationship between the methylation status of the SYN2 gene and schizophrenia was investigated. The aim is to obtain crucial results for illuminating the effects of the SYN2 methylation changes in the etiology of schizophrenia.\u0000 \u0000 \u0000 \u0000 In light of this scientific information, we investigated the methylation status of three different CpG regions in the promoter of the SYN2 gene and compared them in healthy controls and schizophrenia patients. Thirty-three healthy controls and 36 schizophrenia patients were included in this study. Sequencing was performed using the pyrosequencing method to reveal the methylation pattern.\u0000 \u0000 \u0000 \u0000 As a result of the statistical analysis, it was confirmed that there is a significant relationship between the methylation pattern of the SYN2 gene and schizophrenia. Schizophrenia patients showed more methylation in position 2 and position 3. Additionally, the average methylation ratio is increased in schizophrenia patients.\u0000 \u0000 \u0000 \u0000 We find an association between the DNA methylation pattern of the SYN2 gene and schizophrenia. These results can help to the understanding of the etiology of schizophrenia. Except for these, DNA methylation changes in the SYN2 gene in people who live in urban and rural areas can be one of the reasons for the different incidences of schizophrenia in these regions.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"6 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141044654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
COVID-19 in it is more severe form is characterized by a hyperinflammatory condition, hypercoagulation state and the appearance of pulmonary microembolism. In this study we wanted to correlate levels of D-Dimer, protein C, protein S and antithrombin 3 with severity of disease and clinical outcome. We included 134 of patients who were divided in 3 groups regarding oxygen support (high flow oxygen therapy, mechanical ventilation and oxygen supplementation with nasal cannula or mask). Concentration of D-Dimer, and activity of protein C and antithrombin 3 are presented as mean±SD and differed significantly between patients on mechanical ventilation (3.26 ± 1.15 mg/L, 86 ± 22.55 %, 81.21 ± 17.61 %)/HFNO (2.35 ± 1.68 mg/L, 109.6 ± 26.96 %, 94.67 ± 17.49 %)/BNC (1.37 ± 1.17 mg/L, 116.92 ± 28.16 %, 103.29 ± 15.63 %) with p<0.001 for all parameters. Mortality in oxygen group was 10.9 %, in HFNC group 40.7 % and in mechanical ventilated group 80 %. determination of anticoagulant factors in COVID-19 patients may indicate which of them are at increased risk of developing severe disease, venous thromboembolism and fatal clinical outcome.
{"title":"Activity of protein C, protein S and antithrombin 3 in COVID-19 patients treated with different modalities of oxygen supplementation","authors":"Šavuk Ana, Grizelj Danijela, Svaguša Tomo, Čulo Melanie-Ivana, Zagorec Nikola, Šakota Sara, Orehovec Biserka, Kelava Tomislav, Livun Ana, Marković Ivan, Baković Josip, Kereš Tatjana","doi":"10.1515/tjb-2023-0119","DOIUrl":"https://doi.org/10.1515/tjb-2023-0119","url":null,"abstract":"\u0000 \u0000 \u0000 COVID-19 in it is more severe form is characterized by a hyperinflammatory condition, hypercoagulation state and the appearance of pulmonary microembolism. In this study we wanted to correlate levels of D-Dimer, protein C, protein S and antithrombin 3 with severity of disease and clinical outcome.\u0000 \u0000 \u0000 \u0000 We included 134 of patients who were divided in 3 groups regarding oxygen support (high flow oxygen therapy, mechanical ventilation and oxygen supplementation with nasal cannula or mask).\u0000 \u0000 \u0000 \u0000 Concentration of D-Dimer, and activity of protein C and antithrombin 3 are presented as mean±SD and differed significantly between patients on mechanical ventilation (3.26 ± 1.15 mg/L, 86 ± 22.55 %, 81.21 ± 17.61 %)/HFNO (2.35 ± 1.68 mg/L, 109.6 ± 26.96 %, 94.67 ± 17.49 %)/BNC (1.37 ± 1.17 mg/L, 116.92 ± 28.16 %, 103.29 ± 15.63 %) with p<0.001 for all parameters. Mortality in oxygen group was 10.9 %, in HFNC group 40.7 % and in mechanical ventilated group 80 %.\u0000 \u0000 \u0000 \u0000 determination of anticoagulant factors in COVID-19 patients may indicate which of them are at increased risk of developing severe disease, venous thromboembolism and fatal clinical outcome.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"23 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140658080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The proliferation of vascular smooth muscle cells (VSMCs) induced by hyperglycemia plays a pivotal role in the development of atherosclerosis and restenosis. This study aims to examine the impact of angiotensin II (Ang II) and high glucose on VSMC proliferation and the phosphorylation status of key signalling proteins, specifically ERK1/2, Akt, and STAT3. Furthermore, we assess the inhibitory effects of resveratrol, a polyphenolic compound, on these signalling pathways. Primary vascular smooth muscle cells (VSMCs) isolated from rat aortas were cultured in both standard media (SM: 5.5 mM) and high glucose media (HGM: 25 mM) and then treated with Ang II (100 nM). Proliferation was assessed using the WST-1 assay, and protein analysis was performed through immunoblotting. Ang II increased VSMC proliferation by 39 % in standard glucose environments and 17 % in high glucose environments. Resveratrol effectively suppressed Ang II-induced VSMC proliferation in both media. Furthermore, resveratrol inhibited the phosphorylation of ERK1/2 and Akt. Ang II also induced STAT3 phosphorylation by 29 and 18.5 % in SM and HGM, respectively. However, resveratrol treatment reduced STAT3 phosphorylation to control levels. These findings demonstrated that resveratrol reduces VSMC proliferation induced by Ang II and high glucose conditions, exerting its inhibitory effects by suppressing ERK1/2, Akt, and STAT3 phosphorylation. These results provide valuable insights into the cardioprotective properties of resveratrol.
高血糖诱导的血管平滑肌细胞（VSMC）增殖在动脉粥样硬化和再狭窄的发展中起着关键作用。本研究旨在探讨血管紧张素 II（Ang II）和高血糖对血管平滑肌细胞增殖和关键信号蛋白（特别是 ERK1/2、Akt 和 STAT3）磷酸化状态的影响。此外，我们还评估了多酚化合物白藜芦醇对这些信号通路的抑制作用。 从大鼠主动脉分离的原代血管平滑肌细胞（VSMC）在标准培养基（SM：5.5 mM）和高葡萄糖培养基（HGM：25 mM）中培养，然后用 Ang II（100 nM）处理。用 WST-1 试验评估增殖情况，并通过免疫印迹进行蛋白质分析。 在标准葡萄糖环境中，Ang II 使血管内皮细胞增殖增加了 39%，在高糖环境中增加了 17%。白藜芦醇能有效抑制 Ang II 诱导的血管内皮细胞增殖。此外，白藜芦醇还抑制了 ERK1/2 和 Akt 的磷酸化。在 SM 和 HGM 中，Ang II 还分别诱导 STAT3 磷酸化 29% 和 18.5%。然而，白藜芦醇处理可将 STAT3 磷酸化降至控制水平。 这些研究结果表明，白藜芦醇通过抑制 ERK1/2、Akt 和 STAT3 磷酸化，减少了 Ang II 和高血糖条件下诱导的血管内皮细胞增殖。这些结果为白藜芦醇的心脏保护特性提供了宝贵的见解。
{"title":"Resveratrol modulates signalling to inhibit vascular smooth muscle cell proliferation induced by angiotensin II and high glucose","authors":"A. Çetin, Mustafa Kırça, A. Yeşilkaya","doi":"10.1515/tjb-2023-0191","DOIUrl":"https://doi.org/10.1515/tjb-2023-0191","url":null,"abstract":"\u0000 \u0000 \u0000 The proliferation of vascular smooth muscle cells (VSMCs) induced by hyperglycemia plays a pivotal role in the development of atherosclerosis and restenosis. This study aims to examine the impact of angiotensin II (Ang II) and high glucose on VSMC proliferation and the phosphorylation status of key signalling proteins, specifically ERK1/2, Akt, and STAT3. Furthermore, we assess the inhibitory effects of resveratrol, a polyphenolic compound, on these signalling pathways.\u0000 \u0000 \u0000 \u0000 Primary vascular smooth muscle cells (VSMCs) isolated from rat aortas were cultured in both standard media (SM: 5.5 mM) and high glucose media (HGM: 25 mM) and then treated with Ang II (100 nM). Proliferation was assessed using the WST-1 assay, and protein analysis was performed through immunoblotting.\u0000 \u0000 \u0000 \u0000 Ang II increased VSMC proliferation by 39 % in standard glucose environments and 17 % in high glucose environments. Resveratrol effectively suppressed Ang II-induced VSMC proliferation in both media. Furthermore, resveratrol inhibited the phosphorylation of ERK1/2 and Akt. Ang II also induced STAT3 phosphorylation by 29 and 18.5 % in SM and HGM, respectively. However, resveratrol treatment reduced STAT3 phosphorylation to control levels.\u0000 \u0000 \u0000 \u0000 These findings demonstrated that resveratrol reduces VSMC proliferation induced by Ang II and high glucose conditions, exerting its inhibitory effects by suppressing ERK1/2, Akt, and STAT3 phosphorylation. These results provide valuable insights into the cardioprotective properties of resveratrol.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140684054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayşe Nalbantsoy, Ekin Varol, Ayşe Dila Çaglar, B. Yücel
The bee stinger is the defense organ of honeybees. The venom sac of a worker bee is connected to its stinger, which is used as a defense mechanism, and it has a potent and complex combination of substances that is unique in the animal kingdom. Many immune-related illnesses have been successfully treated with bee venom and recent evidence on the efficacy of applications targeting malignancies has attracted considerable attention. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test was used to determine the cytotoxicity of the crude venom, and the flow cytometric analysis was used to determine the apoptotic potential. The cytotoxic activity of Apis mellifera cypria venom collected from two different apiaries in Cyprus was evaluated for the first time against breast (MDA-MB-231), colon (Caco-2), cervix (HeLa), prostate (PC-3), pancreas (Panc-1), lung (A549), glioblastoma (U-87MG) human cancerous and healthy lung fibroblast (CCD-34Lu) cells. The venom concentration that killed 50 % of the cells (inhibitory concentration, IC50) is expressed as venom cytotoxicity. The IC50 values of A. m. cypria crude venom on cultured cells varied from 4.18±0.75 to 22.00±1.71 μg/mL after treatment with crude venom for 48 h, with the most potent activities against PC-3, Panc-1, and HeLa cells. Analysis of apoptotic cells by flow cytometry of both venom samples showed that bee venom slightly induced early apoptosis on A549 and Panc-1 cells. The venom of the A. m. cypria is discussed in this article, displaying promising results as a potential source for an alternative treatment method because of its cytotoxic effect.
蜂刺是蜜蜂的防御器官。工蜂的毒囊与蜂刺相连，蜂刺是一种防御机制，它具有动物界独一无二的强效和复杂的物质组合。许多与免疫有关的疾病都成功地用蜂毒治疗过，最近关于针对恶性肿瘤的应用疗效的证据引起了人们的极大关注。 本研究采用 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-溴化四氮唑（MTT）试验测定粗蜂毒的细胞毒性，并采用流式细胞分析法测定细胞凋亡潜能。首次评估了从塞浦路斯两个不同养蜂场采集的蜜蜂毒液对乳腺（MDA-MB-231）、结肠（Caco-2）、宫颈（HeLa）、前列腺（PC-3）、胰腺（Panc-1）、肺（A549）、胶质母细胞瘤（U-87MG）人类癌细胞和健康肺成纤维细胞（CCD-34Lu）的细胞毒性活性。 杀死 50% 细胞的毒液浓度（抑制浓度，IC50）表示毒液的细胞毒性。用粗毒处理 48 小时后，A. m. cypria 粗毒对培养细胞的 IC50 值从 4.18±0.75 到 22.00±1.71 μg/mL 不等，其中对 PC-3、Panc-1 和 HeLa 细胞的活性最强。用流式细胞仪分析两种毒液样本的凋亡细胞显示，蜂毒可轻微诱导 A549 和 Panc-1 细胞早期凋亡。 本文讨论了A. m. cypria的毒液，由于其细胞毒性作用，它作为一种替代治疗方法的潜在来源显示出了良好的效果。
{"title":"Cytotoxic and apoptotic effectiveness of Cypriot honeybee (Apis mellifera cypria) venom on various cancer cells","authors":"Ayşe Nalbantsoy, Ekin Varol, Ayşe Dila Çaglar, B. Yücel","doi":"10.1515/tjb-2023-0109","DOIUrl":"https://doi.org/10.1515/tjb-2023-0109","url":null,"abstract":"\u0000 \u0000 \u0000 The bee stinger is the defense organ of honeybees. The venom sac of a worker bee is connected to its stinger, which is used as a defense mechanism, and it has a potent and complex combination of substances that is unique in the animal kingdom. Many immune-related illnesses have been successfully treated with bee venom and recent evidence on the efficacy of applications targeting malignancies has attracted considerable attention.\u0000 \u0000 \u0000 \u0000 The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test was used to determine the cytotoxicity of the crude venom, and the flow cytometric analysis was used to determine the apoptotic potential. The cytotoxic activity of Apis mellifera cypria venom collected from two different apiaries in Cyprus was evaluated for the first time against breast (MDA-MB-231), colon (Caco-2), cervix (HeLa), prostate (PC-3), pancreas (Panc-1), lung (A549), glioblastoma (U-87MG) human cancerous and healthy lung fibroblast (CCD-34Lu) cells.\u0000 \u0000 \u0000 \u0000 The venom concentration that killed 50 % of the cells (inhibitory concentration, IC50) is expressed as venom cytotoxicity. The IC50 values of A. m. cypria crude venom on cultured cells varied from 4.18±0.75 to 22.00±1.71 μg/mL after treatment with crude venom for 48 h, with the most potent activities against PC-3, Panc-1, and HeLa cells. Analysis of apoptotic cells by flow cytometry of both venom samples showed that bee venom slightly induced early apoptosis on A549 and Panc-1 cells.\u0000 \u0000 \u0000 \u0000 The venom of the A. m. cypria is discussed in this article, displaying promising results as a potential source for an alternative treatment method because of its cytotoxic effect.\u0000","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"17 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140715967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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