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Analytical Hierarchy Process for the Selection of A Square: the Case Study of Konya City A广场选择的层次分析法——以科尼亚市为例
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1942-1946.6330
Büşra Altay, Nurgül Arısoy
Rapid population growth, industrial and technological development, and improvement in the social and economical conditions of people have increased their need for socializing, gathering, and relaxing with various recreational activities and mutual communications. The sustainable development of social life has increased the importance of squares as public spaces, which brings the citizens together for cultural, commercial, and political purposes; thus, giving an identity to the city and becoming the focal point of urban life. The selection of an area as a square is based on certain criteria. The decisions regarding the choice of the location and their use as squares must be per the internationally accepted criteria. We studied four squares, namely Mevlana Square with historical background, Hükümet Square, Anıt Square, and Kılıçarslan City Square with a high demand for social events. The squares are considered to be important and comprehensive titles for evaluating their comparative functions under the selection criteria of visuality, functionality, and accessibility. The Analytical Hierarchy Process (AHP) method was used to determine the importance of the selection criteria of squares in a survey with participants for solving the problem and selecting the best square according to these criteria. By performing the AHP analysis, we found that the most preferred square by the participants was the Mevlana Square with a preference rate of 58.68%, and the most preferred criterion was “visuality” with a preference rate of 64.5%. In this study, we aimed to determine the characteristics of a preferable square to improve the existing squares and to contribute to the stages of a new square design, planning, and implementation.
人口的迅速增长、工业和科技的发展以及社会经济条件的改善,增加了人们通过各种娱乐活动和相互交流进行社交、聚会和放松的需求。社会生活的可持续发展增加了广场作为公共空间的重要性,它为文化、商业和政治目的将公民聚集在一起;因此,赋予城市身份,成为城市生活的焦点。选择一个区域作为正方形是基于一定的标准。有关场地的选择及其作为广场的使用的决定必须符合国际公认的标准。我们研究了四个广场,分别是具有历史背景的Mevlana广场、h k广场、Anıt广场和社会活动需求高的Kılıçarslan城市广场。在视觉性、功能性和可达性的选择标准下,方块被认为是评价其比较功能的重要的综合性标题。采用层次分析法(AHP)确定问卷调查中方块选择标准对解决问题的重要性,并根据这些标准选择最佳方块。通过AHP分析,我们发现参与者最喜欢的广场是Mevlana广场,偏好率为58.68%,最喜欢的标准是“可视性”,偏好率为64.5%。在这项研究中,我们旨在确定一个更好的广场的特征,以改善现有广场,并为新广场的设计、规划和实施阶段做出贡献。
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引用次数: 0
Modeling of The Nitrogen Requirement of Winter Wheat for Protein Content Using Optical Sensor in Central Anatolia Region of Türkiye 利用光学传感器模拟<s:1>基耶省中部安纳托利亚地区冬小麦需氮对蛋白质含量的影响
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1958-1965.6427
Uğur Yegül, Burak Şen, Savaş Kuşcu, Ufuk Türker
Chlorophyll meters manage both the amount and duration of nitrogen fertilizer application based on the principle that the chlorophyll or nitrogen content of plants should be maintained throughout their development. For smallholders in developing countries, the use of a hand-held meter to manage nitrogen fertilizer in rice and wheat is the most popular method. The adoption of nitrogen management strategies based on close sensing using chlorophyll meters and optical sensors will largely depend on the inclusion of specific economic analysis in future research. The importance of using sensors and chlorophyll meters for nitrogen fertilizer management depends on how successful current practices have been. In this study, five different nitrogen rates (0, 80, 120, 160, 200 kg N ha-1) were applied to two different wheat varieties, and the effect of these different nitrogen rates on wheat protein content was investigated in a randomized block design. A quadratic polynomial model described the relationship between protein content and nitrogen rates.
叶绿素计根据植物在整个发育过程中保持叶绿素或氮含量的原则来管理氮肥施用量和施肥期。对于发展中国家的小农来说,使用手持式计量器管理水稻和小麦的氮肥是最流行的方法。采用基于叶绿素计和光学传感器的近距离遥感氮管理策略将在很大程度上取决于在未来的研究中纳入具体的经济分析。在氮肥管理中使用传感器和叶绿素计的重要性取决于当前实践的成功程度。采用随机区组设计,研究了5种不同施氮量(0、80、120、160、200 kg N ha-1)对2个小麦品种小麦蛋白质含量的影响。用二次多项式模型描述了蛋白质含量与氮速率之间的关系。
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引用次数: 0
Determination of Resistance of Winter Wheat Varieties Against Root and Crown Rot Fusarium culmorum Under the Artificial Drought Conditions 人工干旱条件下冬小麦品种对根腐病和冠腐病抗性的测定
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1966-1972.6439
Fatih Özdemir
This study was conducted to investigate the relationship between the recent increase in the frequency of drought conditions and Root-Crown Rot (Fusarium pseudograminearum, Fusarium culmorum) in rainfed wheat growing areas of Central Anatolia. In 2018, the experiment was established in the greenhouse of Konya Bahri Dağdaş International Agricultural Research Institute using 25 registered wheat varieties in a randomised block split-plot experimental design with 4 replications. Irrigation levels (100% field capacity and 50% field capacity) were designed as main plots, inoculation (+ and -) as subplots and varieties as sub-subplots. In the study, the response of the cultivars to inoculation under artificial drought conditions was evaluated by measuring Crown Score (CR), Lesion Length (LL), Number of Diseased Leaves from outside to inside (NDL) and Plant Height (PH) from five plants in each pot. Statistically, the differences between inoculation, irrigation, NDL and LL were found to be significant at P<0.0001 level, while the differences between varieties were found to be significant at P<0.001 level for the CR parameter. On the other hand, when the interactions were evaluated for the NDL parameter, the differences were found to be significant at P<0.0001 level for all three interactions of cultivar*inoculation, cultivar*irrigation and cultivar*inoculation*irrigation. When the effect of reduced water application intended to be used in resistance breeding was evaluated for 25 different varieties under Fusarium culmorum inoculation, there was an increase in CR from 41,7% (Gerek-79) to 487,5% (Altay-2000), NDL from 7,14% (Kirgiz-95) to 200% (Alpu-2001), LL from -36,84% (Karahan-99) to 283,33% (Altay-2000) and in PH reduction from 12,41% (Seval) to 32,22% (Kirgiz-95). The results showed that drought-stressed plants were already weakened and therefore more easily and severely infected by pathogens. According to these results, it has been determined that it is very important for the region to obtain resistance to drought and crown rot diseases, which have such an obvious relationship, in breeding studies.
本研究旨在探讨安纳托利亚中部旱作小麦种植区近年来干旱频率的增加与根冠腐病(镰刀菌,镰刀菌)的关系。2018年,该试验在Konya Bahri Dağdaş国际农业研究所的温室中建立,采用25个注册小麦品种,随机区隔小区试验设计,4个重复。灌溉水平(100%田间容量和50%田间容量)为主区,接种(+和-)为副区,品种为副区。在人工干旱条件下,通过测定每盆5个植株的冠值(CR)、病变长度(LL)、由外向内病叶数(NDL)和株高(PH)来评价各品种对接种的响应。统计学上,接种、灌溉、NDL和株高在P<0.0001水平上差异显著;而品种间的差异在P<0.001水平上是显著的。另一方面,对NDL参数进行互作评价时发现,品种*接种、品种*灌溉和品种*接种*灌溉3种互作在P<0.0001水平上差异显著。对25个不同品种接种镰刀菌进行抗性育种的减水量效果评价,CR从41.7% (Gerek-79)增加到487.5% (altayi -2000), NDL从7.14% (Kirgiz-95)增加到200% (Alpu-2001), LL从- 36.84% (Karahan-99)增加到283.33% (altayi -2000), PH从12.41% (Seval)减少到32.22% (Kirgiz-95)。结果表明,受干旱胁迫的植物已经变得虚弱,因此更容易和严重地受到病原体的感染。根据这些结果,确定了具有如此明显关系的干旱和冠腐病对该地区育种研究的重要意义。
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引用次数: 0
Determination of Yield and Quality Parameters in Pickling Hot Peppers Grown under Different Water Stress Conditions 不同水分胁迫条件下酸洗辣椒产量及品质参数的测定
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1953-1957.6404
Okan Erken, Fatma Çolak Levent
Plants are exposed to various adverse environmental conditions throughout their growth period. In recent years, drought, which has occurred and necessitated different measures, ranks among these adverse conditions. At the same time, plants synthesize certain biochemical compounds in response to the adverse conditions they will encounter. These compounds not only strengthen the immune system but also provide resistance against various diseases, and they tend to increase under adverse environmental conditions that plants will face during cultivation. This study was conducted to determine the changes in yield and some biochemical components in pickling hot peppers (Capsicum Annuum L.) grown under different water stress conditions. Two different levels of water stress (%70 and %30 irrigation) were applied in addition to full irrigation (%100 irrigation). At the end of the research, while a yield of 269.42 g per plant was obtained in the control group (%100 irrigation), 150.14 g and 93.33 g of pickling hot peppers were harvested in each water stress treatment, respectively. Total phenolic compound levels increased with water stress; it was determined to be 0.827 mg-1g in the trial irrigated with full irrigation water, 1.170 mg-1g in plants exposed to mild water stress, and up to 1.536 mg-1g in the trial subjected to severe water stress. In addition, total flavonoids and antioxidant compound levels also increased with increasing water stress. The amounts of flavonoid compounds obtained from the trial groups were 0.146, 0.373, and 0.412 mg-1g, respectively, while the antioxidant levels determined by the DPPH method increased in quantity with increasing water stress, similar to other biochemical compounds. According to these results, it was determined that the yield of pickling hot peppers decreased in the case of water shortage that the plants would face in cultivation, but there was an increase in some biochemical compounds.
植物在整个生长时期都暴露在各种不利的环境条件下。近年来,干旱是这些不利条件之一,干旱已经发生,需要采取不同的措施。同时,植物合成某些生化化合物,以应对它们将遇到的不利条件。这些化合物不仅可以增强免疫系统,还可以抵抗各种疾病,并且在植物种植过程中面临的不利环境条件下,它们往往会增加。研究了不同水分胁迫条件下酸洗辣椒(Capsicum Annuum L.)产量及生化指标的变化。除了完全灌溉(%100灌溉)外,还施用了两种不同水平的水分胁迫(%70和%30灌溉)。研究结束时,对照组(%100灌溉)的单株产量为269.42 g,而每个水分胁迫处理的酸洗辣椒产量分别为150.14 g和93.33 g。总酚类化合物水平随水分胁迫而升高;经测定,全灌条件下,其含量为0.827 mg-1g;轻度水分胁迫下,其含量为1.170 mg-1g;重度水分胁迫下,其含量最高可达1.536 mg-1g。此外,总黄酮和抗氧化化合物水平也随水分胁迫的增加而升高。各组黄酮类化合物含量分别为0.146、0.373和0.412 mg-1g, DPPH法测定的抗氧化水平随水分胁迫的增加而增加,与其他生化化合物相似。根据这些结果,可以确定,在栽培中面临缺水的情况下,酸洗辣椒的产量会下降,但一些生化物质会增加。
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引用次数: 0
Molecular Investigation of the Impact of Thermal Processing Techniques on Tropomyosin Crustacean Allergens 热加工技术对原肌球蛋白甲壳类过敏原影响的分子研究
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1926-1934.6313
Elif Tuğçe Aksun Tümerkan
While shellfish species are widely consumed due to their nutritional advantages, they are also among the top eight food items for food-borne allergies. Five distinct thermal processing techniques were applied to the crustacean to investigate the tropomyosin level variations caused by heat processing. Fresh shrimp and prawns were utilized as controls for the determination of allergen-encoding genes. Prior to molecular analysis, the proximate composition and acidity of raw and processed samples were also performed. The yield and purity of DNA were also determined. Melting curve and gel electrophoresis tests verified the existence of allergen-coding genes. Thermal processing procedures affected the proximate composition, particularly the total protein and fat concentrations, according to the findings. Following the heat treatment, the pH levels decreased, particularly in the grilled samples. There were also significant differences in the quantity and quality of the extracted DNA. Regardless of crustacean species, the tropomyosin-encoding gene was detected in both fried and grilled samples. These findings demonstrated that RT-PCR identification and validation of the crustacean allergy gene by gel electrophoresis might be a reliable approach for the thermally treated shrimp and prawn samples. This study shows that investigating the allergen coding gene might provide a viable way for detecting food-borne allergens in other thermally processed food items, which are becoming more concerned about food safety.
虽然贝类因其营养优势而被广泛食用,但它们也是食源性过敏的前八种食物之一。采用五种不同的热处理技术对甲壳类动物进行热处理,研究热处理引起的原肌球蛋白水平变化。以鲜虾和对虾为对照,测定变应原编码基因。在分子分析之前,还进行了原料和加工样品的近似组成和酸度。测定了DNA的产率和纯度。熔融曲线和凝胶电泳检测证实了过敏原编码基因的存在。根据研究结果,热处理过程影响了近似组成,特别是总蛋白质和脂肪浓度。在热处理之后,pH值下降,特别是在烤过的样品中。在提取DNA的数量和质量上也存在显著差异。无论何种甲壳类动物,原肌球蛋白编码基因在油炸和烧烤样品中均被检测到。这些结果表明,凝胶电泳技术对甲壳类动物过敏基因的RT-PCR鉴定和验证可能是一种可靠的方法。该研究表明,对过敏原编码基因的研究可能为其他日益受到关注的食品安全问题中食源性过敏原的检测提供一种可行的方法。
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引用次数: 0
Clinical Effect of Poly Herbal Unani Formulation on Dyslipidemia- A Randomized Trial 复方乌尼方治疗血脂异常的临床疗效——随机试验
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1892-1904.6185
Khairul Alam, Hasib Sheikh, Md. Abdus Samad
Background: In adults aged 30-70 with primary and moderate hyperlipidemia, the present study took place to investigate the therapeutic benefits of a polyherbal unani preparation called Garlitab. Methods: It was a prospective open label, herbal coded test drug-controlled, randomized trial. Out of total screened patients we were enrolled 212 hyperlipidemic patients of 30–70 years in the study fulfilling the inclusion criteria, we were recruited them from OPD of a hospital in Munshiganj and different Unani clinics of Dhaka, Bangladesh after obtaining written informed consent from the patients. Selected individuals were allocated into two groups at random. Group1 Received 500 mg Garlitab tablets twice daily and Group 2 received tablet atorvastatin calcium 10 mg 2 times daily. Height, weight, and blood pressure were recorded along with blood samples. The random distributions were carried out by a research assistant utilizing a random numbers table. Blood samples were taken at the beginning of the trial, 1.5 months later, and 3 months following the intervention. Results: Results for the test medication revealed a substantial drop in cholesterol levels between baseline and the data collected after three months and in case of male it was from 241.72±38.11 to 218.24±34.06 mg/dL for total cholesterol, from 198.27±30.57 to 173.54±29.34 mg/dL for LDL and from 280.78±85.81 to 207.07±51.40 mg/dL for triglyceride. HDL increases from 33.05±3.21 to 34.69±3.13 mg/dL in male patients. The control drug atorvastatin calcium also showed a significant decrease in lipids between baseline and after 3 months data and in case of male it was from 241.92±31.54 to 174.90±22.87 mg/dL for total cholesterol, from 196.20±30.91 to 130.30±24.29 mg/dL for LDL and from 279.48±115.35 to 141.27±59.55 mg/dL for triglyceride. It increases HDL from 32.00±2.25 to 34.03±2.19 mg/dL in male patients. Between the baseline and the 3-month data, the test medicine for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 244.64±52.18 to 220.12±45.07 mg/dL, from 200.32±30.57 to 173.54±29.34 mg/dL and from 272.32±99.69 to 195.25±60.68 mg/dL respectively. HDL increases from 33.77±3.36 to 35.03±3.23 mg/dL. Between the baseline and the 3-month data, the control medication for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 247.74±37.95 to 175.26±29.54 mg/dL, from 197.65±27.89 to 130.91±22.04 mg/dL and from 271.57±94.52 to 142.00±50.88 mg/dL respectively. It increases HDL from 32.22±2.32 to 33.46±2.94 mg/dL. Conclusions: According to the results of the study, the polyherbal formulation Garlitab can lower cholesterol levels. It may be a useful medication for treating primary hyperlipidemia.
背景:在30-70岁患有原发性和中度高脂血症的成年人中,本研究调查了一种名为Garlitab的多草药unani制剂的治疗效果。方法:前瞻性开放标签、草药编码试验、药物对照、随机试验。在所有筛选的患者中,我们招募了212名符合纳入标准的30-70岁的高脂血症患者,我们在获得患者的书面知情同意后,从Munshiganj一家医院的门诊和孟加拉国达卡的不同Unani诊所招募了他们。选定的个体被随机分为两组。组1患者给予加立他片500 mg,每日2次;组2患者给予阿托伐他汀钙片10 mg,每日2次。身高、体重、血压和血液样本一起被记录下来。随机分布由一名研究助理利用随机数表进行。在试验开始时、1.5个月后和干预后3个月采集血样。结果:测试药物的结果显示,在基线和三个月后收集的数据之间,胆固醇水平大幅下降,在男性中,总胆固醇从241.72±38.11降至218.24±34.06 mg/dL, LDL从198.27±30.57降至173.54±29.34 mg/dL,甘油三酯从280.78±85.81降至207.07±51.40 mg/dL。男性患者HDL从33.05±3.21 mg/dL增加到34.69±3.13 mg/dL。对照药物阿托伐他汀钙也显示,在基线和3个月后的数据中,男性的总胆固醇从241.92±31.54降至174.90±22.87 mg/dL, LDL从196.20±30.91降至130.30±24.29 mg/dL,甘油三酯从279.48±115.35降至141.27±59.55 mg/dL。使男性患者HDL从32.00±2.25 mg/dL增加到34.03±2.19 mg/dL。在基线和3个月的数据之间,女性试验药物显著降低总胆固醇、低密度脂蛋白和甘油三酯,分别从244.64±52.18 mg/dL降至220.12±45.07 mg/dL,从200.32±30.57降至173.54±29.34 mg/dL和从272.32±99.69降至195.25±60.68 mg/dL。HDL从33.77±3.36 mg/dL增加到35.03±3.23 mg/dL。在基线和3个月的数据之间,女性对照药物显著降低了总胆固醇、低密度脂蛋白和甘油三酯,分别从247.74±37.95 mg/dL降至175.26±29.54 mg/dL,从197.65±27.89降至130.91±22.04 mg/dL和从271.57±94.52降至142.00±50.88 mg/dL。使HDL从32.22±2.32 mg/dL增加到33.46±2.94 mg/dL。结论:复方Garlitab具有降低胆固醇水平的作用。它可能是治疗原发性高脂血症的有效药物。
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引用次数: 0
Analysis of Monthly Precipitation at the Basin Scale in Türkiye 浙江流域月降水分析
Pub Date : 2023-10-24 DOI: 10.24925/turjaf.v11i10.1973-1985.6445
Hasan Hüseyin Aksu
Basin-based water management strategy is one of the necessary instruments for the protection and sustainable use of water resources against climate change. In this paper, the monthly precipitation distributions of the 25 major basins in Türkiye were produced, and amounts and volumes were computed and analyzed. Only annual modeling and assessments of precipitation may hide months with precipitation shortages. Empirical Bayesian Kriging (EBK), Ordinary Kriging (OK), and Inverse Distance Weighting (IDW) were implemented in interpolation. EBK outperformed in all months and calculations were based on the EBK. The month with the highest precipitation potential in Türkiye is December (77.9 mm, 60.77 billion m3), and the month with the lowest precipitation potential is August (13.8 mm, 10.76 billion m3). In the basins, the monthly precipitation amounts range between 2.7 mm and 185.2 mm, and the volumes range between 0.02 billion m3 and 13.24 billion m3. The basins with the highest precipitation depth were determined as the East Black Sea, Antalya, Asi, and Ceyhan, and the lowest as the Small Menderes, Konya, and Tigris-Euphrates in different months. The monthly precipitation patterns and potentials of the basins vary widely. In May, June, July, August, and September, when water, particularly agricultural irrigation, is required the most, the 20 basins, except for the 5 located in Northern Türkiye precipitation shortage was determined.
基于流域的水资源管理战略是保护和可持续利用水资源应对气候变化的必要手段之一。本文对云南25个主要流域的月降水分布进行了计算和分析。只有对降水的年度模拟和评估才能隐藏降水短缺的月份。采用经验贝叶斯克里格法(EBK)、普通克里格法(OK)和逆距离加权法(IDW)进行插值。EBK在所有月份都表现出色,计算基于EBK。全年降水潜力最大的月份是12月(77.9 mm, 607.7亿m3),降水潜力最小的月份是8月(13.8 mm, 107.6亿m3)。流域月降水量在2.7 ~ 185.2 mm之间,年降水量在0.02 ~ 132.4亿m3之间。不同月份降水深度最大的是东黑海、安塔利亚、阿西和杰伊汉流域,最小的是小门德雷斯、科尼亚和底格里斯河-幼发拉底河流域。流域的月降水模式和潜力变化很大。5月、6月、7月、8月和9月是用水尤其是农业灌溉需水量最大的季节,除北部5个流域外,其余20个流域均存在降水短缺。
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引用次数: 0
Seroprevalence of Bovine Leukemia Virus Infection in Cattle in Muş Province, Türkiye 牛白血病病毒感染在穆乌省牛血清中的流行病学研究
Pub Date : 2023-10-23 DOI: 10.24925/turjaf.v11i10.1878-1881.6275
Alaattin Sökmen, Ali Rıza Babaoğlu
Bovine leukemia virus (BLV) is known as the causative agent of enzootic bovine leukosis (EBL), which is a worldwide distributed disease and has also been detected in marketed beef and dairy products. BLV causes significant economic losses due to the loss of milk and yield or the slaughter of animals without adequate development. It has been reported in epidemiological studies that this infection is common in Türkiye, especially in the western provinces. There is no data on the possible presence or prevalence of BLV infection and its seroepidemiology in Muş province. The aim of this study is to determine the possible presence and prevalence of the infection, its role in yield losses, and to obtain epidemiological data on cattle farming in the Muş district. For this purpose, 300 blood serum samples were collected from cattle aged six months and older in the province of Muş and its different districts. The blood serum samples taken were tested for the presence of BLV-specific antibodies by agar gel immunodiffusion (AGID) and competitive enzyme-linked immunosorbent assay (C-ELISA) methods. As a result of the study, all of the controlled districts were evaluated as negative in the AGID and C-ELISA tests for the presence of BLV-specific antibodies. In conclusion, for the first time, it was demonstrated that cattle farming in the Muş province were BLV-free during the sampling period. Although BLV seropositivity was not detected in the tested animals, it is emphasized that the control of infection and eradication program should not be ignored.
牛白血病病毒(BLV)被认为是引起地方性牛白血病(EBL)的病原体,EBL是一种世界范围内分布的疾病,也在市场上销售的牛肉和乳制品中被检测到。由于乳汁和产量的损失或未充分发育的动物被屠宰,BLV造成了重大的经济损失。据流行病学研究报告,这种感染在基耶病毒病中很常见,特别是在西部省份。没有关于穆乌伊省可能存在或流行BLV感染及其血清流行病学的数据。本研究的目的是确定这种感染的可能存在和流行程度、其在产量损失中的作用,并获取muul地区养牛的流行病学数据。为此目的,从穆穆伊省及其不同地区的6个月及以上的牛身上收集了300个血清样本。采用琼脂凝胶免疫扩散法(AGID)和竞争性酶联免疫吸附法(C-ELISA)检测血清中blv特异性抗体的存在。研究结果显示,所有对照区在AGID和C-ELISA检测中blv特异性抗体均为阴性。综上所述,首次证明在采样期间,穆乌省的养牛业没有blv。虽然在试验动物中未检测到BLV血清阳性,但强调感染控制和根除计划不应忽视。
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引用次数: 0
Effect of Potassium Humate on Soybean Germination Traits Under Salinity Stress Conditions 盐胁迫条件下腐植酸钾对大豆发芽性状的影响
Pub Date : 2023-10-23 DOI: 10.24925/turjaf.v11i10.1889-1891.6236
Öner Canavar, Hatice Kübra Gören, Seçil Küçük Kaya, Feride Öncan Sümer
This study investigated the effects of salinity and K-humate concentrations on soybean germination. The findings contribute to our knowledge of soybean germination under salt stress and the potential use of potassium humate. The experiment was conducted in the laboratory of the Department of Field Crops at Adnan Menderes University, Turkey. Seeds were surface-sterilized and placed on filter paper in Petri dishes. Different concentrations of water (control), NaCl solution (3 dS m-1), and K-humate solution were added. The experiment followed a factorial design with two factors and four replications. K-humate had a positive impact on germination rate. Significant differences were observed among control, salinity, and salinity * K-humate applications. Salinity reduced germination percentage. K-humate mitigated the negative effects of salinity, improving germination. These findings suggest the potential use of K-humate to enhance seedling establishment and overall plant productivity in salinity-affected environments.
研究了盐度和腐植酸钾浓度对大豆发芽的影响。这些发现有助于我们了解盐胁迫下大豆发芽和腐植酸钾的潜在用途。该实验是在土耳其Adnan Menderes大学大田作物系实验室进行的。种子表面消毒,放在滤纸上,放在培养皿中。加入不同浓度的水(对照)、NaCl溶液(3ds - m-1)和k -腐植酸盐溶液。本试验采用因子设计,有2个因子和4个重复。腐植酸钾对发芽率有正向影响。对照、盐度和盐度* k -腐植酸盐施用之间存在显著差异。盐度降低了发芽率。腐植酸钾减轻了盐度的负面影响,提高了发芽率。这些发现表明,在受盐分影响的环境中,腐植酸钾具有提高幼苗成活率和植物整体生产力的潜力。
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引用次数: 0
Callus formation and camphor aggregation in response to sorbitol stimulated osmotic stress in yarrow 山梨糖醇刺激渗透胁迫对蓍草愈伤组织形成和樟脑聚集的影响
Pub Date : 2023-10-23 DOI: 10.24925/turjaf.v11i10.1882-1888.6328
Muhammed Akif Açıkgöz, Ahmet Aygün, Ebru Batı Ay, Şevket Metin Kara
Sorbitol is an important source of abiotic stress that is used to increase osmolality in cell cultures. It increases the antioxidant enzymes of defense catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) in the stress state of cells. Sorbitol plays an important role in stimulating these enzymes in cells and increasing phenylalanine ammonium lyase (PAL) activity. The aim of this study was to apply increasing doses of sorbitol elicitor to cell suspension cultures to determine the changes in cell number, viability, dry weight, and camphor content. In vitro plantlets were obtained from plant seeds and stem segments of these plants were used as explant source. Cell cultures were established after callus formation. Then, 0 (control), 5, 25, and 50 g L-1 sorbitol was dissolved in distilled water and cultured. Samples were taken three times in total, starting from day 1 to day 3. The content of camphor was detected by gas chromatography-mass spectrometry (GC-MS). Cell number, viability,dry weight, and camphor content increased significantly with increasing doses of sorbitol compared to sampling times. Compared to the initial culture, the amount of camphor increased by 40% at the 5 g L-1 dose, 82% at the 25 g L-1 dose, and 154% at the 50 g L-1 dose. In A. gypsicola cell cultures, increasing doses of sorbitol have clearly demonstrated the secondary metabolite accumulation and its positive effect on cell growth.
山梨糖醇是一种重要的非生物应激源,用于增加细胞培养中的渗透压。增加应激状态下细胞抗氧化酶防御过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)。山梨糖醇在刺激细胞内这些酶和提高苯丙氨酸解铵酶(PAL)活性方面起着重要作用。本研究的目的是在细胞悬浮培养中增加山梨糖醇激发剂的剂量,以确定细胞数量、活力、干重和樟脑含量的变化。以植物种子和茎段为外植体,获得离体植株。愈伤组织形成后进行细胞培养。然后将0(对照)、5、25和50 g L-1山梨醇溶于蒸馏水中培养。从第1天到第3天,共取样3次。采用气相色谱-质谱联用技术检测樟脑的含量。与取样次数相比,随着山梨醇剂量的增加,细胞数量、活力、干重和樟脑含量显著增加。与初始培养相比,5 g L-1剂量时樟脑的数量增加了40%,25 g L-1剂量增加了82%,50 g L-1剂量增加了154%。在A. gypsicola细胞培养中,增加山梨醇的剂量清楚地证明了次生代谢物的积累及其对细胞生长的积极作用。
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Turkish Journal of Agriculture: Food Science and Technology
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