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Kenya Medical Training College laboratory student perception on adequacy of teaching and learning resources in the advent of COVID-19 肯尼亚医学培训学院实验室学生对COVID-19到来时教学资源充分性的看法
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.7
Benson Mbati Kondo, Simon Kang’ethe, Anne Ngeno
The emergence of COVID-19 presented a big challenge in medical education, especially with regard to the adequacy of teaching and learning resources. In addition, an increase in student intake and demand for Kenya Medical Training College courses could compromise these resources. This study investigated the perceptions of students towards the adequacy of teaching and learning resources at Kenya Medical Training College in the advent of COVID-19. The general objective was to determine the perceived adequacy of teaching and learning resources in the advent of COVID-19 by students in the department of Medical Laboratory Sciences at Kenya Medical Training College. A descriptive cross-sectional study design using mixed methods (triangulation) was used. Simple random sampling of the eight regions in the country was used to select one region (the Eastern Region). Census sampling was used to select all the four campuses in the region namely Machakos, Meru, Embu, and Kitui, which offer medical laboratory sciences. The study population was students in the department of medical laboratory sciences on these campuses, with 220 students participating. The data collection instrument was a self- administered closed- and open-ended questionnaires. Quantitative data collected was analysed using SPSS software, while qualitative data was analysed using themes. The response rate was 100% (220 students). The gender distribution among students showed a higher percentage in male (58%). Majority of the students were 21-23 years (35%). One-way ANOVA presented a statistically significance between and within groups (P<0.05). Majority of students (51.8%) were not satisfied with adequacy of human resources. Majority of students perceived the state of physical facilities to be in poor state with a mean of 1.76, while the satisfaction level of majority of students’ on teaching aids was below 3.40 representing very inadequate to neutral. Ordinal regression tests on satisfaction level on teaching aids in relation to gender, year of study and campus was significant (chi-square test p<0.000) while the goodness of fit model was (p=1.000) indicating it was a good model. R^2 value (Nagelkerke=99%) indicated that the study factors had great variations. The spearman’s rank test showed that laboratory equipment and computer assisted aids, internet, learning systems have negative correlation but with very strong coefficient correlation of 1.0000. Satisfaction levels with teaching aids in relation to gender, year of study, and campus were significant with a chi-square test of P < 0.000. The study concluded that there were inadequate teaching and learning resources on the four KMTC campuses before COVID-19.
新冠肺炎疫情的出现给医学教育带来了巨大的挑战,特别是在教学资源的充足性方面。此外,学生人数的增加和对肯尼亚医学培训学院课程需求的增加可能会损害这些资源。本研究调查了在COVID-19出现时,学生对肯尼亚医学培训学院教学资源充足性的看法。总体目标是确定肯尼亚医学培训学院医学实验室科学系的学生在COVID-19出现时认为教学资源是否充足。采用混合方法(三角测量)的描述性横断面研究设计。在全国八个地区进行简单随机抽样,选择一个地区(东部地区)。利用人口普查抽样选择了该地区提供医学实验室科学的所有四个校区,即马查科斯、梅鲁、恩布和基图伊。研究对象是这些大学医学实验室科学系的学生,共有220名学生参与。数据收集工具是一个自我管理的封闭式和开放式问卷。定量数据采用SPSS软件分析,定性数据采用主题分析。回复率为100%(220名学生)。在学生的性别分布中,男性的比例更高(58%)。年龄以21 ~ 23岁为主(35%)。单因素方差分析显示组间和组内差异有统计学意义(P<0.05)。大多数学生(51.8%)不满意人力资源是否充足。大部分学生认为物理设施的状况较差,平均为1.76,而大部分学生对教具的满意度在3.40以下,表示非常不足至中性。性别、年级、校园对教具满意度的排序回归检验有显著性(χ 2检验p<0.000),拟合优度模型为(p=1.000),表明模型良好。R^2值(Nagelkerke=99%)表明各研究因素差异较大。spearman秩次检验表明,实验室设备与计算机辅助设备、网络、学习系统呈负相关,但有很强的系数相关(1.000)。通过P <的卡方检验,对教具的满意度水平与性别、学习年份和校园有关;0.000. 该研究得出结论,在新冠肺炎之前,四个校区的教学资源不足。
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引用次数: 0
The effects of prenatal exposure to varying doses of pantoprazole on the maternal and fetal outcomes in albino rats (<i>rattus norvegicus</i>) 产前暴露于不同剂量泮托拉唑对白化大鼠(褐家鼠)母胎结局的影响(&lt;i&gt; /i&gt;)
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.4
Anne Njoki Nyaga, Joseph Kariuki Kweri, James Mwangi Kanyoni, Ann Wairimu Mwangi, Caroline Chepng’eno Sigei, Jennifer Chepkemoi Segut, Jane Wanjiru Kuria
Pantoprazole is a proton pump inhibitor used in the management of hypergastric secretions and gastroesophageal reflux during pregnancy. However, its prenatal effects on maternal and fetal outcomes are not well reported when administered at varying doses and at different gestational periods. A post-test-only experimental study design was adopted in conducting this study. A sample size of 30 female albino rats was used for the study. The 30 albino rats were grouped into two broad study categories: 3 control rats and 27 treatment rats. The 27 treatment rats were subdivided into three study groups of nine rats each according to the doses administered as follows: 9 low-dose rats, 9 medium-dose rats, and 9 high-dose rats The nine rats assemblies were further divided up into three subgroups, each of three rats, according to the time of exposure, as follows: three rats for trimester one, three rats for trimester two, and three rats for trimester three. Daily maternal weights were recorded every morning, and then at gestation day 20, all animals were humanely sacrificed and the fetuses harvested. Continuous data included the maternal and fetal weights, and discrete data included the litter sizes, number of devoured fetuses, resorbed glands, and number of embryolithalities. Data was recorded, coded, and entered in the computer using MS Excel spreadsheets version 13, and analyzed using the SPSS programme for Windows version 25 (one-way Analysis of Variance (ANOVA) followed by Tukey’s post hoc multiple comparisons test). The results were expressed as means ± standard error of the mean (SEM). Results with a P < 0.05 were considered significant in the study. This study observed that pantoprazole, at high doses, was associated with a decrease in the mean maternal weight gain, reduced litter sizes with increased numbers of resorbed endometrial glands, and devoured fetuses.
泮托拉唑是一种质子泵抑制剂,用于治疗妊娠期间胃高分泌物和胃食管反流。然而,在不同的剂量和不同的妊娠期,其对母体和胎儿结局的产前影响尚未得到很好的报道。本研究采用后验式实验研究设计。本研究采用30只雌性白化大鼠作为样本。将30只白化病大鼠分为两大类:对照组3只,治疗组27只。27只给药大鼠按给药剂量分为3组,每组9只:低剂量9只,中剂量9只,高剂量9只。9只大鼠按受药时间又分为3个亚组,每组3只:妊娠1期3只,妊娠2期3只,妊娠3期3只。每天早晨记录每日母体体重,妊娠第20天人道处死所有动物,收获胎儿。连续数据包括母胎体重,离散数据包括产仔数、被吞噬胎儿数、被吸收腺体数和胚胎结石数。使用MS Excel电子表格版本13记录、编码和输入数据,并使用Windows版本25的SPSS程序进行分析(单向方差分析(ANOVA),随后进行Tukey事后多重比较检验)。结果用均数±均数标准误差(SEM)表示。P <0.05被认为在研究中具有显著性。本研究观察到,高剂量的泮托拉唑与母体平均体重增加减少、产仔数减少、子宫内膜腺吸收数量增加和吞噬胎儿有关。
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引用次数: 0
Evaluation of crushed slates as a suitable capping material for rapid gravity sand filters 碎板岩作为快速重力砂过滤器盖层材料的评价
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.5
Maina Oscar Njung’e, Beatrice Katheu Kakoi, Njenga Mburu
In the potable water treatment process, Rapid Gravity Sand Filters (RGSF) are commonly adopted as the last solid-liquid separation stage. Cleaning of the RGSF is done through backwashing. RGSF is widely adopted all over the World due to its ease of operation and high filtration rates. However, these filters suffer from stratification of the sand media, which causes floc removal to occur only at the topmost layer of the filter bed, leaving the remaining depth unutilized. Capping is a technique whereby a thin layer of sand filter media is replaced with a suitable coarse material to overcome the problem of stratification and transform a singlemedia RGSF into a dual-media filter. The objective of this study is to determine the suitability of crushed slates as a capping material. The study evaluated the performance of a crushed expanded slate-capped filter against a conventional single-media RGSF, the effects of its physical and chemical characteristics, and varying the depth of the capping material. Laboratory tests were conducted to assess the physical and chemical characteristics of slates from Maji ya Chumvi (Coast, Kenya). This included specific gravity, acid solubility, water extractable substances, silica content, and friability. A performance comparison was carried out by means of a fabricated model filtration unit set up within an existing community water treatment plant. The model filtration unit was fed with pretreated raw water of varying influent turbidities. Crushed expanded slates met the minimum physical and chemical requirements for use as a capping material for RGSF. The crushed expanded slate-capped RGSF model demonstrated high robustness under high shock turbidity loads (above 150 NTU), which is illustrated by an increased length of filter run of 27% (50–150 NTU) and 45% (150–300 NTU). Increasing the depth of capping material from 25mm to 50mm did not yield any significant improvement or deterioration in the filter run length. At influent turbidities below 150 NTU, the effluent water quality for all three scenarios (uncapped, 25 mm, and 50mm crushed expanded slates capped) is below 5 NTU and therefore meets the Kenyan drinking water standards. Above 150 NTU influent turbidity, the effluent water quality for the uncapped RGSF deteriorates, whereas for the 25mm and 50mm capped RGSF, it remains consistently below 5 NTU. This demonstrates the usefulness of the crushed expanded slates in improving the turbidity removal of RGSF for high (above 150 NTU) turbidity loads. This study recommends a full-scale trial of crushed expanded slates to facilitate a more precise estimation of the overall benefit of full-scale community water filtration systems.
在饮用水处理过程中,通常采用快速重力砂过滤器(RGSF)作为最后一个固液分离阶段。RGSF的清洗是通过反冲洗来完成的。RGSF因其易于操作和高过滤率而在世界范围内被广泛采用。然而,这些过滤器受到砂介质分层的影响,这导致絮凝体只发生在过滤床的最上层,而剩余的深度未被利用。封盖是一种技术,通过将薄层砂过滤介质替换为合适的粗层材料来克服分层问题,并将单介质RGSF转变为双介质过滤器。本研究的目的是确定碎板作为封盖材料的适用性。该研究评估了破碎膨胀板盖过滤器与传统单介质RGSF的性能,其物理和化学特性的影响,以及封盖材料深度的变化。进行了实验室测试,以评估Maji ya Chumvi(肯尼亚海岸)石板的物理和化学特性。这包括比重、酸溶解度、水萃取物质、二氧化硅含量和脆性。通过在现有社区水处理厂内设置的预制模型过滤单元进行性能比较。模型过滤单元用不同浊度的预处理原水进行进水。破碎的膨胀板满足RGSF封盖材料的最低物理和化学要求。破碎膨胀板盖RGSF模型在高冲击浊度负荷(超过150 NTU)下表现出很高的鲁棒性,这表明过滤器运行长度增加了27% (50-150 NTU)和45% (150 - 300 NTU)。将封盖材料的深度从25mm增加到50mm并没有产生任何显著的改善或恶化的过滤器运行长度。进水浊度低于150 NTU时,所有三种情况(未封盖、25毫米和50毫米压碎膨胀板封盖)的出水水质均低于5 NTU,因此符合肯尼亚饮用水标准。超过150 NTU的进水浊度,未封顶的RGSF的出水水质恶化,而对于25毫米和50毫米封顶的RGSF,它始终保持在5 NTU以下。这证明了破碎膨胀板在提高RGSF在高(高于150 NTU)浊度负荷下的除浊性方面的有用性。本研究建议对破碎的膨胀板进行全面试验,以便更准确地估计全面社区水过滤系统的整体效益。
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引用次数: 0
Augmenting climate-resilient energy infrastructure through National and International Standards for Renewables 通过可再生能源国家和国际标准增强气候适应型能源基础设施
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.8
Francis Xavier Ochieng, Joseph Ngugi Kamau, Ephantus Mbugua Kamweru
Kenya’s Energy sector has an installed capacity of 3.601 GW (grid-connected 3.321 GW and captive 280.76 MW), with 80.48% coming directly from Renewable energy sources. This huge percentage of renewable energy in the energy mix, however, is not reflected in the adoption or development of Standards. Standards help enhance the safety and efficiency of products, security, and quality assurance while ensuring interconnectivity and interoperability of components. Standards enable the dissemination of new technologies, good practises, and ultimately a climate-resilient energy infrastructure (CREI). To achieve CREI, standards act as a catalyst for advancing the attainment of economies of scale for renewables while also providing technical specifications to accelerate their deployment. This study thus addresses the ominous gap that exists due to the lack of a proper standard inventory across all renewables. In this way, this work addresses the irregular and at times inconsistent gap between the direct causal link between policy and standards on the one side and application areas such as management, information systems, and social use of renewable energy on the other. The study employed the Mixed Methods Research (MMR) approach to enable an understanding of the Renewable Energy (RE) standards environment in Kenya. In addition, realistic literature reviews and metaanalysis literature reviews were employed to deal with complex standards within the renewable energy sector. The study demonstrated that in the majority of Renewable Energy Technologies (RETs), major gaps in terms of standards exist for various RETs, the import of this being that the majority of RETs cannot be manufactured locally. The study also concludes that a low awareness exists of standard implementation, and further, due to a lack of awareness of technological innovations on the global level, the development of national standards and capacity building of competent staff (including techno-financial support for renewable energy technologies) have been significantly hampered. Subsequently, the study does appreciate the role of universities and higher educational institutions as being central in the research and analysis of renewable energy technologies as well as the adaptation and/or adoption of local and international standards.
肯尼亚能源部门的装机容量为3.601吉瓦(并网发电3.321吉瓦,自备发电280.76兆瓦),其中80.48%直接来自可再生能源。然而,可再生能源在能源结构中所占的巨大比例并没有反映在标准的采用或制定中。标准有助于提高产品的安全性和效率、安全性和质量保证,同时确保组件的互联性和互操作性。标准有助于新技术、良好实践的传播,最终实现气候适应型能源基础设施(CREI)。为了实现CREI,标准起到了促进可再生能源实现规模经济的催化剂作用,同时也提供了加速其部署的技术规范。因此,这项研究解决了由于缺乏所有可再生能源的适当标准库存而存在的不祥差距。通过这种方式,这项工作解决了政策和标准之间的直接因果关系与可再生能源的管理、信息系统和社会利用等应用领域之间不规律的、有时不一致的差距。该研究采用了混合方法研究(MMR)方法,使人们能够了解肯尼亚的可再生能源(RE)标准环境。此外,采用现实文献综述和元分析文献综述来处理可再生能源领域内的复杂标准。研究表明,在大多数可再生能源技术中,各种可再生能源技术在标准方面存在重大差距,这是因为大多数可再生能源技术不能在当地制造。该研究还得出结论,对标准实施的认识很低,而且,由于在全球一级缺乏对技术创新的认识,国家标准的制定和主管人员的能力建设(包括对可再生能源技术的技术财政支持)受到了严重阻碍。随后,这项研究确实认识到大学和高等教育机构在研究和分析可再生能源技术以及适应和/或采用当地和国际标准方面的核心作用。
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引用次数: 0
Characterization and classification of soil resources of hittinahalli sub-watershed derived from basalt in Northern dry zone of Karnataka 卡纳塔克邦北部干旱区玄武岩衍生hittinahalli亚流域土壤资源特征与分类
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.2
Parameshgouda Lavanagouda Patil, Deepa Kalappanavar, G.P. Geetha, Ragini Patil, G.S. Adarsh, Ghulappa S. Dasog
Eighteen Typical pedons representing upland, midland, and lowland forms in a Hittinahalli subwatershed of Vijayapur district were studied for their morphological characteristics and physicochemical properties. The soils were shallow to very deep (25 to >150 cm), very dark brown to black (Munsell color chart) and moderately well to poorly drained. The soil structure changes from weak, medium sub- angular blocky on the surface to moderate, coarse sub-angular blocky in the subsurface horizons. The soils are moderately alkaline to  strongly alkaline (7.59–9.41), low to high in organic carbon (0.08–0.75 g/kg). The pedons on uplands exhibit the development of an argillic horizon (Bt). The pedons on the midlands and lowlands have cambic horizons (Bw), classified as Verisols and Inceptisols, respectively. The  lowlands (P6, P10, P16, and P18) and midlands (P7, P8, P12, P15, and P17) are classified as fine, mixed, Iso-hyperthermic, and Typic  Haplustalfs.
本文研究了维查亚普尔地区Hittinahalli亚流域18个典型的高地、中部和低地类型的土壤形态特征和理化性质。土壤浅至深(25至150厘米),深棕色至黑色(蒙塞尔颜色图),排水良好至不良。土壤结构由表层的弱中亚角块状向地下层的中粗亚角块状转变。土壤呈中碱性至强碱性(7.59 ~ 9.41),有机碳含量由低至高(0.08 ~ 0.75 g/kg)。高地上的土墩呈泥质层发育(Bt)。中部和低地的土丘具有形成纪层(Bw),分别被分类为Verisols和Inceptisols。低地(P6、P10、P16、P18)和中部(P7、P8、P12、P15、P17)可分为细粒、混合型、等温型和典型单峰。
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引用次数: 0
Apple flower-visiting insects’ diversity and abundance in selected central Kenya orchards 苹果访花昆虫在肯尼亚中部果园的多样性和丰度
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.3
Ruth Moraa Anyieni, Josephine Muthoni Karanja, Mary Wanjiku Gikungu, Nicolas J. Vereecken
Flower-visiting insects are essential in maintaining a healthy and productive agricultural landscape through ecosystem services such as pollination. Fruits are important sources of vitamins and micronutrients, and several fruit crops depend on animal pollination, which enhances their nutritional content. Besides honey bees, apple orchards can sustain a large number of arthropod species that also aid in pollination. In this present study, we assessed the diversity and abundance of insect flower visitors in six apple orchards in Nyeri and Laikipia East. Six plots, each with more than 20 apple trees, were purposefully selected. Data were collected through timed visual searches and sweep netting. Sampling was done from 0900 hours to 1700 hours, six days a week, for five months consecutively in 2019. Diversity indices were computed using the Shannon-Wiener diversity index, while abundance patterns were displayed using species abundance curves. Differences in species diversity and abundance between sites were compared using a one-way analysis of variance. A total of 1,221 insects belonging to 23 families, 82 species, and 4 orders (Hymenoptera, Lepidoptera, Coleoptera, and Diptera) were recorded. The order Hymenoptera (bees, wasps, and ants) was the most abundant, with 1,176 individuals, and had the most species-rich taxa, with 56 species in 9 families. The order Coleoptera (beetles) was second, with 26 individuals and 12 species in 6 families. Lepidoptera were the least abundant with 10 individuals and the least species-rich taxa with 7 species in 5 families, followed closely by Diptera with 7 individuals and 7 species in 3 families. There were significant differences in flower visitors’ composition among the six sites. The study provides important information on the status of key apple flower visitors, which can guide orchard management practises to increase apple yield through pollinator conservation. Conserving apple pollinators will enhance fruit production, promote the livelihoods of farmers, and contribute to the national economy.
访花昆虫通过授粉等生态系统服务对维持健康和多产的农业景观至关重要。水果是维生素和微量营养素的重要来源,一些水果作物依赖动物授粉,这增加了它们的营养成分。除了蜜蜂,苹果园还可以维持大量的节肢动物物种,这些物种也有助于授粉。本研究对尼耶里和莱基皮亚东部6个苹果园昆虫访花昆虫的多样性和丰度进行了研究。有目的地选择了六个地块,每个地块都有20多棵苹果树。通过定时目视搜索和扫网收集数据。2019年,采样时间从早上9点到下午17点,每周6天,连续5个月。多样性指数采用Shannon-Wiener多样性指数计算,丰度模式采用物种丰度曲线显示。采用单因素方差分析比较了不同地点间物种多样性和丰度的差异。共记录昆虫1221只,隶属膜翅目、鳞翅目、鞘翅目、双翅目23科82种。膜翅目(蜂、蜂、蚁)昆虫数量最多,有1176只,类群最丰富,有9科56种。鞘翅目(甲虫)次之,有6科12种26只;鳞翅目最不丰富,有5科7种,其次是双翅目,有7种,3科7种。6个样地的赏花游客组成差异显著。该研究为了解苹果主要访花昆虫的现状提供了重要信息,可以指导果园管理实践,通过保护传粉昆虫来提高苹果产量。保护苹果传粉媒介将提高果实产量,促进农民生计,并为国民经济做出贡献。
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引用次数: 0
Common occupational machinery hazards in mechanical engineering workshops in TVET institutions in Nairobi metropolitan, Kenya 肯尼亚内罗毕大都市TVET机构机械工程车间常见职业机械危害
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.9
Patrick Kipkurui Ronoh, Charles M. Mburu
The metal fabrication sector involves a variety of processes, activities, products, and by-products. This involves various interventions such as milling, turning, welding, drilling, and grinding. Firms in this sector use one or a combination of these interventions where machinery is used, which can expose workers to machinery hazards if proper safety procedures are not observed. Occupational Safety and Health (OSH) is vital in Technical Vocational Education and Training (TVET) institutions, especially in mechanical engineering programmes where metal fabrication is practised. The objective of this study was to assess the awareness of occupational machinery hazards in mechanical engineering workshops in TVET Institutions. The study adopted a descriptive research design and employed a structured questionnaire for data collection. Purposive sampling was used to identify institutions participating in the study. SPSS version 25 was used to analyse the data and present it in tables and graphs. Noise (90.4%) and vibration (71.9%) were reported as the most common occupational hazards, respectively, in mechanical engineering workshops. Regarding workstations, the grinding section (39.4%) and milling section (15.8%) were reported as experiencing high levels of noise. The study recommends that adequate control measures be put in place to mitigate against these hazards.
金属制造部门涉及各种工艺、活动、产品和副产品。这涉及各种干预,如铣削,车削,焊接,钻孔和磨削。该部门的公司在使用机械时使用一种或几种干预措施,如果不遵守适当的安全程序,可能会使工人暴露于机械危害中。职业安全与健康(OSH)在职业技术教育和培训(TVET)机构中至关重要,特别是在从事金属加工的机械工程方案中。本研究的目的是评估职业技术教育机构机械工程车间对职业机械危害的认知。本研究采用描述性研究设计,采用结构化问卷进行数据收集。目的抽样用于确定参与研究的机构。采用SPSS version 25对数据进行分析,并以图表形式呈现。噪声(90.4%)和振动(71.9%)分别是机械工程车间最常见的职业危害。在工作站方面,磨削部分(39.4%)和铣削部分(15.8%)被报告为高水平的噪音。该研究建议采取适当的控制措施来减轻这些危害。
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引用次数: 0
Effects of different rangeland management practices on vegetation metrics and wildlife abundance in a semi-closed ecosystem in north-central Kenya 肯尼亚中北部半封闭生态系统中不同牧场管理措施对植被指标和野生动物丰度的影响
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.1
Timothy N. Kaaria, Catherine N. Ngamau, David W. Kimiti
Rangelands contribute at least 30% of terrestrial net primary productivity, making them an important part of natural ecosystems despite low and unpredictable rainfall regimes. Rangelands are sensitive to anthropogenic activities, making management interventions key to maintaining forage quality and quantity for wildlife. This study explored the effects of mowing of grasslands and carrying away (MO), prescribed grazing (PG), and unprescribed grazing (UG) on above-ground biomass, basal gaps, and wildlife abundance at Lewa Wildlife Conservancy in Meru, Kenya. Data collection was done 18 months after treatment for PG and MO, while UG was continuous. Treated blocks were selected in a systematic and random way, while adjacent untreated plots acted as controls. Blocks were divided into 100 m × 100 m grid cells using ArcMap 10.8.1, where sampling plots were drawn. T-statistics and analysis of variance (ANOVA) tests were used to test statistical significance. We found a significant reduction in the aboveground biomass between MO and its control (t = 4.886, p = 0.003) and between UG and its control (t =5.487, p = 0.007). No significant change was observed between PG and its control (t = 1.192, p = 0.287). MO increased wildlife abundance (t = -4.670, p = 0.003), while PG (t = 0.589, p = 0.583) and UG (t = -0.262, p = 0.803) showed no difference compared to their controls. The mean length of basal gaps between MO and its control decreased (t = 7.069, p = 0.001), while those between UG and its control increased (t = -4.053, p = 0.001), with no effect observed between PG and its control (t = 1.882, p = 0.061). This study recommends the use of mowing of grasslands and carrying away on rangelands as it positively influence the metrics under investigation.
牧场贡献了至少30%的陆地净初级生产力,使其成为自然生态系统的重要组成部分,尽管降雨量低且不可预测。牧场对人为活动很敏感,因此管理干预是维持野生动物饲料质量和数量的关键。在肯尼亚梅鲁的Lewa野生动物保护区,研究了刈割草地和搬运(MO)、规定放牧(PG)和非规定放牧(UG)对地上生物量、基间距和野生动物丰度的影响。数据收集于PG和MO治疗后18个月,UG持续治疗。以系统和随机的方式选择处理过的地块,而相邻的未经处理的地块作为对照。使用ArcMap 10.8.1将区块划分为100 m × 100 m网格单元,并绘制采样图。采用t统计和方差分析(ANOVA)检验检验统计学显著性。我们发现,MO和UG的地上生物量显著减少(t = 4.886, p = 0.003), UG的地上生物量显著减少(t =5.487, p = 0.007)。PG与对照组无显著差异(t = 1.192, p = 0.287)。MO增加了野生动物丰度(t = -4.670, p = 0.003), PG (t = 0.589, p = 0.583)和UG (t = -0.262, p = 0.803)与对照组相比无显著差异。MO与对照组的平均基底间隙长度减小(t = 7.069, p = 0.001), UG与对照组的平均基底间隙长度增大(t = -4.053, p = 0.001), PG与对照组无显著差异(t = 1.882, p = 0.061)。本研究建议在牧场上使用割草和运走,因为它对所调查的指标有积极影响。
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引用次数: 0
Determination of gold nanoparticles sizes from their plasmon resonance within the optical spectrum 光谱内等离子体共振测定金纳米颗粒大小
Pub Date : 2023-08-02 DOI: 10.4314/jagst.v22i4.6
Mbae John Gitonga, Simon Waweru Mugo, James Mbiyu Ngaruiya
Nanoparticles have exciting properties that can be tailored by altering their size, density, and shape. A number of important properties of the nanoparticles have been investigated for various applications. One such property that is strongly affected by nanoparticle size is localised surface plasmon resonance (LSPR). The resonance from metal nanoparticles has been used in dye-sensitised solar cells to improve their performance. In this work, the dependence of plasmonic properties on nanoparticle sizes is shown. The gold nanoparticles were prepared using a reduction process where hydrogen tetrachloroaurate acid was used as the base gold salt and reduced by sodium citrate at different molarities ranging from 0.015 to 0.035 mol/L. The method produces monodispersed nanoparticles whose sizes are sensitive to the concentration of chemicals used and the completeness of the reduction process. The process took approximately 18 minutes, and the colour changed from pale yellow to wine-red. The absorbance of the resulting gold nanoparticles was determined using a UV-Vis spectrophotometer within the range of 300 nm to 800 nm. The LSPR peaks were found to occur within 518 nm to 520 nm, and from a Gaussian fit, the FWHM ranged from 45.5 to 51.0 nm. The absorption peaks had a narrow range of 14 nm over the range of molarity of sodium citrate. A high molarity concentration of 0.035 mol/L produced a small particle with a diameter of 17.04 nm, while a low concentration of 0.015 mol/L produced a size of 26.55 nm. The interaction of electrons in the specific orbitals, sp- and d-, of nanoparticles exhibited pronounced multiple resonances with the reduction of nanoparticle sizes.
纳米粒子具有令人兴奋的特性,可以通过改变它们的大小、密度和形状来定制。纳米颗粒的许多重要性质已被研究用于各种应用。其中一个受纳米颗粒大小影响很大的特性是局部表面等离子体共振(LSPR)。金属纳米粒子的共振已被用于染料敏化太阳能电池,以提高其性能。在这项工作中,显示了等离子体性质对纳米颗粒尺寸的依赖。以四氯酸氢为基础金盐,用柠檬酸钠在0.015 ~ 0.035 mol/L的浓度范围内还原制备金纳米颗粒。该方法产生的单分散纳米颗粒的大小对所用化学品的浓度和还原过程的完整性敏感。这个过程大约花了18分钟,颜色从淡黄色变成了酒红色。用紫外可见分光光度计测定所得金纳米颗粒在300 ~ 800 nm范围内的吸光度。LSPR峰分布在518 ~ 520 nm范围内,FWHM分布在45.5 ~ 51.0 nm范围内。在柠檬酸钠的摩尔浓度范围内,吸收峰只有14 nm的窄范围。当浓度为0.035 mol/L时,生成的小颗粒直径为17.04 nm,当浓度为0.015 mol/L时,生成的小颗粒直径为26.55 nm。随着纳米粒子尺寸的减小,电子在特定轨道sp-和d-上的相互作用表现出明显的多重共振。
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引用次数: 0
Farklı Hayvan Türlerine Ait Et ve Yüzey Örneklerinden İzole Edilen Staphylococcus aureus Suşlarında Virülens Genlerinin Belirlenmesi
Pub Date : 2023-07-31 DOI: 10.24925/turjaf.v11i7.1238-1244.6090
Murat Karahan, Seyda Şahin, M. Moğulkoç, Recep Kalin
Staphylococcus aureus (S. aureus) insanlarda ve hayvanlarda patojen bir tür olup, dünyadaki gıda kaynaklı zehirlenmelerin en önemli nedenlerinden biridir. Bu çalışmada farklı hayvan türlerine (sığır, koyun, keçi ve tavuk) ait etlerde ve gıdaların temas ettiği yüzeylerden elde edilen örneklerde S. aureus’un koagülaz (coa), clumping faktör (clfA) ve protein A (spa-IgG; spa-X) gibi önemli virülens genlerinin moleküler yöntemlerle karakterize edilmesi amaçlandı. Bu amaçla Sivas ilindeki çeşitli işletmelerden et (n=400) ve yüzey svap (n=50) örnekleri toplandı. Örnekler fenotipik yöntemlerle koagülaz pozitif S. aureus olarak identifiye edildi. Koagülaz pozitif olarak doğrulanan izolatlara ait virülens genleri tekli ve mültipleks Polimeraz Zincir Reaksiyonu (PZR) ile incelendi. Toplamda 450 örneğin 110’unda (%24,4) S. aureus belirlendi. Bu izolatların %29, %24, %14, %32 ve %22 oranlarında sığır, koyun, keçi ve tavuk et örneklerinin yanı sıra yüzey örneklerinde de bulundu. S. aureus izolatlarının tamamında coa, clfA, spa’nın IgG bağlayan bölgesi ve X bölgesini kodlayan genler tespit edildi. Bu dört gen bölgesinin ikisinde polimorfizmler gözlendi. Coa-PZR’da, izolatların tamamında beş farklı moleküler uzunluğa sahip ve 500-1400 bç arasında değişen tek bir bant oluşumu bulundu. spa-X-PZR’da ise izolatların tamamında yedi farklı moleküler uzunluğa sahip ve 190-350 bç arasında değişen tek bir bant oluşumu belirlendi. Sonuç olarak, et ve yüzey orijinli S. aureus izolatlarında coa-PZR ile spa gen polimorfizmleri tespitinin geleneksel yöntemlere karşı iyi bir alternatif olarak kullanılabileceği, dolayısıyla stafilokokal enfeksiyonlara karşı etkili kontrol stratejilerinin geliştirilmesi ve halk sağlığının korunmasına katkı sağlayacağı düşünülmektedir.
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引用次数: 0
期刊
Turkish Journal of Agriculture: Food Science and Technology
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