Ukrains'kyi biokhimichnyi zhurnal最新文献

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[The effect of ATP-dependent K(+)-channel opener on the functional state and the opening of cyclosporine-sensitive pore in rat liver mitochondria]. [atp依赖性K(+)通道开启剂对大鼠肝脏线粒体功能状态及环孢素敏感孔开放的影响]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-05-01 DOI: 10.15407/ubj85.03.038

O. Akopova, V. Nosar, V. Buryĭ, L. I. Kolchinskaia, I. N. Man'kovskaia, V. Sagach

The effect of mitochondrial ATP-dependent K(+)-channel (K+(ATP)-channel) opener diazoxide (DZ) on the oxygen consumption, functional state and the opening of cyclosporine-sensitive pore in the rat liver mitochondria has been studied. It has been established that K+(ATP)-channel activation results in the increase of the oxygen consumption rate (V4(s)) and the uncoupling due to the acceleration of K(+)-cycling, the decrease in state 3 respiration rate (V3) and the respiratory control ratio (RCR). Under K+(ATP)-channel activation an inhibition of oxidative phosphorylation takes place which reduces the rate of ATP synthesis and hydrolysis as well as ATP production and consequently results in the seeming increase of P/O ratio. It has been shown that the increase in ATP-dependent K(+)-uptake accompanied by the opening of mitochondrial permeability transition pore (MPTP) leads to dramatic uncoupling of the respiratory chain due to simultaneous activation of K(+)- and Ca(2+)-cycling supported by MPTP and Ca(2+)-uniporter as well as K(+)-channels and K+/H(+)-exchange. K+(ATP)-channel activation leads to the partial inhibition of MPTP, but insufficient for the restoration of mitochondrial functions. Elimination of Ca(2+)-cycling after MPTP opening is necessary to return mitochondrial functions back to the control level which shows that MPTP could serve as the mechanism of reversible modulation of bioenergetic effects of K+(ATP)-channel activation.

研究了线粒体ATP依赖性K(+)通道(K+(ATP)通道开启剂二氮氧化物(DZ)对大鼠肝脏线粒体耗氧量、功能状态和环孢素敏感孔开放的影响。已经确定K+(ATP)通道激活导致氧气消耗速率(V4(s))的增加和K(+)-循环加速导致的解耦，状态3呼吸速率(V3)和呼吸控制率(RCR)的降低。在K+(ATP)通道激活下，氧化磷酸化发生抑制，从而降低ATP合成和水解的速率以及ATP的产生，从而导致P/O比的表面增加。研究表明，atp依赖性K(+)-摄取的增加伴随着线粒体通透性过渡孔(MPTP)的打开，由于MPTP和Ca(2+)-单转运体以及K(+)-通道和K(+) /H(+)-交换支持的K(+)-和Ca(2+)-循环同时激活，导致呼吸链急剧解偶联。K+(ATP)通道激活导致MPTP部分抑制，但不足以恢复线粒体功能。MPTP打开后Ca(2+)循环的消除是使线粒体功能恢复到控制水平所必需的，这表明MPTP可能是可逆调节K+(ATP)通道激活的生物能量效应的机制。

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引用次数: 3

[Functions of metallothioneins and a system of antioxidant defense under the effect of Co- and Zn-containing nanocomposites on crucian carp (Carassius auratus gibelio)]. [含钴、锌纳米复合材料对异育银鲫金属硫蛋白的作用及抗氧化防御系统研究]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-05-01 DOI: 10.15407/ubj85.03.052

H. I. Fal'fushyns'ka, L. L. Hnatyshyna, O. Turta, O. Stoliar, N. Mitina, O. Zaichenko, R. Stoika

The effect of metal-nanocomposites (Me-NC) of cobalt and zinc (Co- and Zn-NC, correspondingly) synthecized on the basis of vinylpyrrolidone (PS) on the metal-accumulative proteins with antioxidant potential metallothioneins (MT) in crucian carp (Carassius auratus gibelio) was studied. Fish was subjected to the effect of Co-NC, Zn-NC, Co2+, Zn2+ or polymer carrier (PC) in the concentrations correspondent to 50 microg x Co/l or 100 microg x Zn/l during 14 days. It was shown that the MTs response is highly specific for the nature of metal, both in ion and Me-NC form: the effect of Co and Co-NC provoked the elevation of total MT concentration (MT-SH) and activation of antioxidant defence, whereas Zn and Zn-NC induced the decrease of the concentration of MT-SH and the inhibition of antioxidant defense. All the exposures provoked the decrease of the concentration of immunoreactive chelating MT form (MTi) and reduced glutathione, activation of anaerobiosys and Mn-superoxide dismutase, and also decrease of the concentration of proteins and lipids oxidative injury products. It was accompanied by the increase of the content of erythrocytes with nuclear abnormalities but did not cause the decrease of choline esterase activity. According to the rate of MT-SH and MTi concentrations, antioxidant potential of MTs is determined by its apoform. Our data indicate that partial biodegradation of Me-NC occurs in the organism of crucian carp.

研究了以乙烯基吡咯烷酮(PS)为基础合成的钴锌金属纳米复合材料(Me-NC，分别为Co-和Zn-NC)对异育银鲫(Carassius auratus gibelio)中具有抗氧化潜力的金属硫蛋白(MT)的影响。将Co- nc、Zn- nc、Co2+、Zn2+或聚合物载体(PC)分别以50 μ g × Co/l或100 μ g × Zn/l的浓度对鱼进行为期14天的影响。结果表明，无论是离子形式还是Me-NC形式，MT反应都具有高度的特异性:Co和Co- nc的作用引起MT总浓度(MT- sh)的升高和抗氧化防御的激活，而Zn和Zn- nc则引起MT- sh浓度的降低和抗氧化防御的抑制。所有暴露均引起免疫反应性螯合MT (MTi)和还原性谷胱甘肽的浓度降低，厌氧系统和mn超氧化物歧化酶的激活，蛋白质和脂质氧化损伤产物的浓度降低。伴有核异常红细胞含量增加，但未引起胆碱酯酶活性降低。根据MT-SH和MTi浓度的速率，MTs的抗氧化能力由其异构体决定。我们的数据表明，Me-NC在鲫鱼体内发生部分生物降解。

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引用次数: 1

Effect of fullerene C60 on ATPase activity and superprecipitation of skeletal muscle actomyosin. 富勒烯C60对ATPase活性和骨骼肌肌动球蛋白超沉淀的影响。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-03-01 DOI: 10.15407/ubj85.02.020

K. Andreichenko, S. Prylutska, Kateryna O. Medynska, K. Bogutska, N. Nurishchenko, Y. Prylutskyy, U. Ritter, P. Scharff

Creation of new biocompatible nanomaterials, which can exhibit the specific biological effects, is an important complex problem that requires the use of last accomplishments of biotechnology. The effect of pristine water-soluble fullerene C60 on ATPase activity and superprecipitation reaction of rabbit skeletal muscle natural actomyosin has been revealed, namely an increase of actomyosin superprecipitation and Mg2+, Ca(2+)- and K(+)-ATPase activity by fullerene was investigated. We conclude that this finding offers a real possibility for the regulation of contraction-relaxation of skeletal muscle with fullerene C60.

创造具有生物相容性的新型纳米材料是一个重要的复杂问题，它需要利用生物技术的最新成果。揭示了原始水溶性富勒烯C60对兔骨骼肌天然肌动球蛋白atp酶活性和超沉淀反应的影响，即富勒烯增加肌动球蛋白超沉淀和Mg2+、Ca(2+)-和K(+)- atp酶活性。我们的结论是，这一发现为富勒烯C60调节骨骼肌收缩松弛提供了一种真正的可能性。

引用次数: 14

[Peptide composition of extracts of cryopreserved pigs' and piglets' heart fragments]. [猪和仔猪冷冻心脏碎片提取物的肽组成]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-01-01 DOI: 10.15407/ubj85.02.078

L. A. Rohoza, T. S. Diubko, S. I. Hal'chenko, B. P. Sandomyrs'kyĭ

It is known that extracts of cryopreserved organ fragments of pigs and piglets stimulate the processes of reparative regeneration. Therefore, the study of the extracts is essential for understanding the mechanism of their biological effects. In this paper was studied the molecular mass distribution of peptides in the extracts of cryopreserved heart fragments of pigs' and piglets', i.e., the chromatograms of pigs' heart extracts show 3 picks, whilst piglets' heart extract show 6 picks. The peculiarity of pigs' heart extracts is the absence of peptides with molecular mass of 10,000 and more. The differences in the intensity of extracts fluorescence prove that the peptides in pigs' heart extracts contain greater amount of tryptophan residues accessible for solvent. The synchronous fluorescence spectra of extracts were obtained which allows the identification of extract without assessment of their components. Results shown in this research could be used for control and standardization of heart extracts peptide composition under investigation of their biological quality.

众所周知，冷冻保存的猪和仔猪器官碎片提取物可刺激修复性再生过程。因此，对其提取物的研究对了解其生物学作用机制具有重要意义。本文研究了猪和仔猪冷冻心脏片段提取物中多肽的分子质量分布，即猪心脏提取物的色谱为3个峰，仔猪心脏提取物的色谱为6个峰。猪心脏提取物的特点是缺乏分子质量为10,000或更多的肽。提取液荧光强度的差异说明猪心脏提取液中含有较多的可溶色氨酸残基。获得了提取物的同步荧光光谱，可以在不评估其成分的情况下对提取物进行鉴定。本研究结果可用于心脏提取物多肽组成的控制和标准化，并对其生物品质进行研究。

引用次数: 0

[Stress-responsive systems in the rat pancreas upon long-term gastric hypochlorhydria and administration of multiprobiotic "Symbiter"]. [长期胃低氯酸和服用多益生菌“Symbiter”后大鼠胰腺的应激反应系统]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-01-01 DOI: 10.15407/ubj85.02.068

K. Dvorshchenko, S. Vakal, A. S. Dranytsyna, S. Senin, L. Ostapchenko

The intensity of free-radical processes upon long-term omeprazole-induced hypoacidity in the rat pancreas was investigated. Significant violation of oxidative-antioxidative balance in pancreatic tissue upon gastric hypochlorhydria was established: overproduction of superoxide anion, quantitative changes of lipid functional groups, increased level of lipid peroxidation products, augmentation of xanthine oxidase, superoxide dismutase and glutathione transferase activity, as well as depletion of catalase, glutathione peroxidase activity and reduced glutathione content. The inflected expression of Cckbr gene in the rat pancreas upon these conditions was also observed, thus suggesting an increased risk of pathological changes development in the gland. Abovementioned parameters were only partially restored to control values in the case of simultaneous administration of multiprobiotic "Symbiter" with omeprazole, thus indicating the ability of this preparation to efficiently counteract the development of oxidative damages in pancreatic tissues upon long-term hypoacidic conditions.

研究了长期奥美拉唑诱导的大鼠胰腺低酸性自由基过程的强度。胃次氯酸导致胰腺组织氧化-抗氧化平衡明显破坏:超氧阴离子过量产生，脂质官能团数量改变，脂质过氧化产物水平升高，黄嘌呤氧化酶、超氧化物歧化酶和谷胱甘肽转移酶活性升高，过氧化氢酶、谷胱甘肽过氧化物酶活性降低，谷胱甘肽含量降低。在这些条件下，还观察到大鼠胰腺中Cckbr基因的表达受到影响，从而表明腺体发生病理变化的风险增加。在与奥美拉唑同时服用多益生菌“Symbiter”的情况下，上述参数仅部分恢复到控制值，从而表明该制剂能够有效地抵消长期低酸性条件下胰腺组织氧化损伤的发展。

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引用次数: 1

[Laureates of the Palladin Prize of the National Academy of Sciences of Ukraine (1983-1984)]. [乌克兰国家科学院帕拉丁奖获得者(1983-1984)]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-01-01 DOI: 10.15407/ubj86.01.139

R. P. Vynogradova, V. M. Danylova

引用次数: 1

[Membranothropic properties of the urocanic acid]. [尿尿酸的膜性]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 2013-01-01 DOI: 10.15407/ubj85.01.042

N. Kasian, O. Vashchenko, L. N. Zavora, D. Sofronov, L. N. Lisetskii

The effects of urocanic acid (UA) on thermodynamic parameters of model dipalmitoylphosphatidylcholine (DPPC) lipid membrane have been studied by means of differential scanning calorimetry (DSC). The observed ordering effect of UA on the lipid bilayer is reflected in the increase in both the main phase transition temperature and cooperative unit size of the lipid membrane. Analysis of FTIR spectra suggests localization of UA molecules in the vicinity of the polar heads and carbonyl groups of DPPC due to electrostatic interactions and H-bonds. On the basis of experimental data obtained and geometry parameters of UA and DPPC molecules, some variants of the UA localization in DPPC bilayer were discussed.

采用差示扫描量热法(DSC)研究了尿尿酸(UA)对模型双棕榈酰磷脂酰胆碱(DPPC)脂膜热力学参数的影响。所观察到的UA对脂质双分子层的有序作用体现在主相变温度和脂质膜协同单位尺寸的增加上。FTIR光谱分析表明，由于静电相互作用和氢键的作用，UA分子定位在DPPC的极头和羰基附近。根据实验数据和UA和DPPC分子的几何参数，讨论了UA在DPPC双分子层中定位的一些变化。

引用次数: 1

Adenylate cyclase of myometrium and its regulation by cAMP-dependent protein kinase. 肌层腺苷酸环化酶及其camp依赖性蛋白激酶的调控。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 1999-05-01 DOI: 10.1097/00005768-199905001-01517

S. Shlykov, M. Kalinski

The purpose of this study was to examine activity of adenylate cyclase (AC) and its cAMP-dependent phosphorylation by cAMP-dependent protein kinase (PKA) in the membrane of rabbit myometrium. Isoproterenol (IP) significantly increased AC activity of nonpregnant rabbits myometrium plasma membranes. However, during pregnancy AC of myometrium plasma membranes did not respond to IP. Phosphorylation of the myometrium membrane by PKA was followed by significantly decreased response of AC to IP.

本研究的目的是检测兔肌膜中腺苷酸环化酶(AC)的活性及其被camp依赖蛋白激酶(PKA)磷酸化的情况。异丙肾上腺素(IP)可显著提高未妊娠家兔肌层质膜AC活性。然而，在怀孕期间，肌膜质膜AC对IP没有反应。PKA使肌膜磷酸化后，AC对IP的反应显著降低。

引用次数: 0

[Content of free amino acids in rabbit tissues as affected by adrenalectomy and administration of hydrocortisone and ACTH]. [肾上腺切除术、氢化可的松和ACTH对兔组织中游离氨基酸含量的影响]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 1977-11-01

T M Mishunina, A Ia Mestechkina, V F Lisak

Adrenalectomy is accompanied by a decreased content of free amino acids in muscules and blood plasma by an increased content of certain amino acids in the liver. A single injection of hydrocortisone to adrenalectomized animals increases the content of amino acids in muscles and lowers their total content in the liver; a single injection of ACTH lowers the total content of amino acids in the liver and blood plasma "Substitutive therapy" by hydrocortisone essentially increases the content of amino acids in the liver, muscles and blood plasma. Under conditions of the experiment the least changes in the content of amino acids were found in the brain tissue.

肾上腺切除术伴随着肌肉和血浆中游离氨基酸含量的减少和肝脏中某些氨基酸含量的增加。肾上腺切除动物单次注射氢化可的松可增加肌肉中氨基酸的含量并降低其在肝脏中的总含量;单次注射ACTH降低肝脏和血浆中氨基酸的总含量氢化可的松“替代疗法”实质上是增加肝脏、肌肉和血浆中氨基酸的含量。在实验条件下，脑组织中氨基酸含量的变化最小。

引用次数: 0

[Isoenzymic spectra of lactate dehydrogenase and malate dehydrogenase in the loach unfertilized eggs and embryos]. [泥鳅未受精卵和胚胎中乳酸脱氢酶和苹果酸脱氢酶的同工酶谱]。

Ukrains'kyi biokhimichnyi zhurnal

Pub Date : 1977-11-01

S I Kusen', R S Stoĭka, I E Sukhors'ka

Isoenzymic spectra of lactate dehydrogenase and malate dehydrogenase were detected in unfertilized eggs of Misgurnus fossilis. They do not change significantly in the embryos during blastula, gastrula and early organogenesis stages of development. The intraspecific polymorphism of one lactate dehydrogenase subunit was found in the unfertilized eggs and embryos. The polymorphism is pronounced in the existence of three types of isoenzymic spectra of different frequencies in the given population of the loach.

用同工酶谱测定了石竹未受精卵的乳酸脱氢酶和苹果酸脱氢酶。它们在囊胚、原胚和早期器官发生发育阶段的胚胎中没有显著变化。在未受精卵和胚胎中发现了一个乳酸脱氢酶亚基的种内多态性。这种多态性在泥鳅种群中存在三种不同频率的同工酶谱。

引用次数: 0

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