Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.58-67
L. Quoc, H. Anh
Polyscias fruticosa (L.) Harms root extracts were obtained and separated using ultrasound-assisted extraction (UAE) with the preset optimal parameters, such as solvent/solid (SS) ratio (50/1, mL g–1), ethanol concentration (40%, v/v), temperature (45 °C), and extraction time (20 min). The best values of the total phenolic content (TPC) and antioxidant capacity (AC) of the extracts under these extraction conditions were 2.13 ± 0.02 mg of gallic acid equivalents (GAE) per gram of dry sample weight (DW) for TPC and 78.13 ± 0.25% for AC. In addition, the structure of the plant material was examined by scanning electron microscopy (SEM): it was revealed that the structure of the residues changed completely as a result of the ultrasound treatment compared to the initial material.
{"title":"Ultrasound-Assisted Extraction of Phenolic Compounds from Polyscias fruticosa (L.) Harms Root","authors":"L. Quoc, H. Anh","doi":"10.26907/2542-064x.2023.1.58-67","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.58-67","url":null,"abstract":"Polyscias fruticosa (L.) Harms root extracts were obtained and separated using ultrasound-assisted extraction (UAE) with the preset optimal parameters, such as solvent/solid (SS) ratio (50/1, mL g–1), ethanol concentration (40%, v/v), temperature (45 °C), and extraction time (20 min). The best values of the total phenolic content (TPC) and antioxidant capacity (AC) of the extracts under these extraction conditions were 2.13 ± 0.02 mg of gallic acid equivalents (GAE) per gram of dry sample weight (DW) for TPC and 78.13 ± 0.25% for AC. In addition, the structure of the plant material was examined by scanning electron microscopy (SEM): it was revealed that the structure of the residues changed completely as a result of the ultrasound treatment compared to the initial material.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78056421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.68-82
A.R. Chibirev, E.K. Konkova, A. Garifzyanov
To obtain reliable results in the quantitative determination of rare earth elements (REE) by atomic emission spectroscopy, it is particularly important to take into account the matrix effects of the macrocomponents contained in the analyzed solutions. Analytes obtained by liquid-phase and autoclave opening of geological samples of REE ores and minerals contain significant amounts of strong mineral acids used as reagents and such macrocomponents of the samples as aluminum (aluminosilicates) and phosphorus (phosphates in apatites). Here, we studied the effects of hydrochloric, nitric, sulfuric, and orthophosphoric acids and aluminum on the relative intensity of the ion analytical lines of La, Ce, Nd, Sm, Gd, Tb, Er, and Yb in atomic emission spectroscopy of the microwave-induced plasma (AES MIP). With an increase in the acid concentration from 0 to 1 mol/L, the relative intensity of the spectral lines of all investigated REE decreased monotonically by 10–20%. The depressing effect of aluminum, which is due to a decrease in the degree of ionization of REE atoms, was much stronger and reached 70%. It was shown that the AES MIP method is not inferior to atomic emission spectroscopy of inductively coupled argon plasma in terms of the detection limits of lanthanum, cerium, gadolinium, and erbium.
{"title":"Assessment of the Effect of Mineral Acids and Aluminum on the Intensity of Spectral Lines of Rare Earth Elements in Atomic Emission Spectroscopy of Microwave-Induced Plasma","authors":"A.R. Chibirev, E.K. Konkova, A. Garifzyanov","doi":"10.26907/2542-064x.2023.1.68-82","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.68-82","url":null,"abstract":"To obtain reliable results in the quantitative determination of rare earth elements (REE) by atomic emission spectroscopy, it is particularly important to take into account the matrix effects of the macrocomponents contained in the analyzed solutions. Analytes obtained by liquid-phase and autoclave opening of geological samples of REE ores and minerals contain significant amounts of strong mineral acids used as reagents and such macrocomponents of the samples as aluminum (aluminosilicates) and phosphorus (phosphates in apatites). Here, we studied the effects of hydrochloric, nitric, sulfuric, and orthophosphoric acids and aluminum on the relative intensity of the ion analytical lines of La, Ce, Nd, Sm, Gd, Tb, Er, and Yb in atomic emission spectroscopy of the microwave-induced plasma (AES MIP). With an increase in the acid concentration from 0 to 1 mol/L, the relative intensity of the spectral lines of all investigated REE decreased monotonically by 10–20%. The depressing effect of aluminum, which is due to a decrease in the degree of ionization of REE atoms, was much stronger and reached 70%. It was shown that the AES MIP method is not inferior to atomic emission spectroscopy of inductively coupled argon plasma in terms of the detection limits of lanthanum, cerium, gadolinium, and erbium.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76787640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.2.295-307
S. Zorina, K. I. Nikashin, N. Afanasieva, N. Hamada
Quartz grains were separated by standard sieving of the Paleogene sands from the Sosnovka Formation of the Ulyanovsk-Syzran Volga region, which are a scarce and valuable raw material for glass making and construction. In order to establish the origin of the sands and restore the facies conditions of their formation, the surface microtextures of the separated quartz grains were examined by optical and electron microscopy. It was shown that the clastic grains of the Sosnovka sands bear the signs of a multi-stage formation process under the conditions of subaquatic (beach zone), eolian, mixed subaquatic-eolian (coastal dunes), injective, and continental pedological settings. For the first time, a complex of microtextures characteristic of the extrusion of grains from deep horizons under pressure and inherent in the injection mechanism of sand formation was described. The diagnostic complex of injectites includes rotational microtextures, notched microtroughs, and deformed fluid inclusions. The rather fresh appearance of the listed microtextures suggests that they were superimposed on the coastal-marine and eolian stages of the evolution with the subsequent formation of sand intrusions. At the final stage of the geological history of the Sosnovka sands, the grains were shaped by the continental pedological conditions, when secondary silica in the form of globules and their clusters cemented the quartz grains, the surface of which concatenated the features of the previous stages. Based on the results of the study, an interpretation scheme summarizing various microtextural features of the reconstructed paleoenvironments and the stages of formation the Sosnovka sands was developed. The finding that the Sosnovka sands are sand intrusions is of great practical importance, as it necessitates new approaches to the discovery and prediction of glass and quartz sand deposits confined to the Paleogene Sosnovka Formation of the Ulyanovsk-Syzran Volga region.
{"title":"Surface Microtextures of Quartz Grains and Origin of the Paleogene Sands in the Ulyanovsk-Syzran Volga Region","authors":"S. Zorina, K. I. Nikashin, N. Afanasieva, N. Hamada","doi":"10.26907/2542-064x.2023.2.295-307","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.2.295-307","url":null,"abstract":"Quartz grains were separated by standard sieving of the Paleogene sands from the Sosnovka Formation of the Ulyanovsk-Syzran Volga region, which are a scarce and valuable raw material for glass making and construction. In order to establish the origin of the sands and restore the facies conditions of their formation, the surface microtextures of the separated quartz grains were examined by optical and electron microscopy. It was shown that the clastic grains of the Sosnovka sands bear the signs of a multi-stage formation process under the conditions of subaquatic (beach zone), eolian, mixed subaquatic-eolian (coastal dunes), injective, and continental pedological settings. For the first time, a complex of microtextures characteristic of the extrusion of grains from deep horizons under pressure and inherent in the injection mechanism of sand formation was described. The diagnostic complex of injectites includes rotational microtextures, notched microtroughs, and deformed fluid inclusions. The rather fresh appearance of the listed microtextures suggests that they were superimposed on the coastal-marine and eolian stages of the evolution with the subsequent formation of sand intrusions. At the final stage of the geological history of the Sosnovka sands, the grains were shaped by the continental pedological conditions, when secondary silica in the form of globules and their clusters cemented the quartz grains, the surface of which concatenated the features of the previous stages. Based on the results of the study, an interpretation scheme summarizing various microtextural features of the reconstructed paleoenvironments and the stages of formation the Sosnovka sands was developed. The finding that the Sosnovka sands are sand intrusions is of great practical importance, as it necessitates new approaches to the discovery and prediction of glass and quartz sand deposits confined to the Paleogene Sosnovka Formation of the Ulyanovsk-Syzran Volga region.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75149138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.118-132
R. Bakeeva, S. Garmonov, V.D. Osipova, K.V. Chernyj, S.Yu. Mamykina, V. Sopin
It was shown by the conductometric study that the formation of micelles in the cetyltrimethylam¬monium bromide (CTAB) – dimethylsulfoxide (DMSO) – water system occurs at higher critical micelle concentrations (CMC) than in the CTAB – water system. The solubilization of nitroxoline in this system upon reaching the CMC was determined by the spectrophotometric method. The Box–Behnken design was used to obtain systems with the highest light absorption of nitroxoline, depending on the CTAB concentration, the acidity of the pH medium, and the proportion of DMSO when searching for the optimal matrix. A sensitive and selective technique suitable for micellar media and spectrophotometric analysis was developed using the response surface methodology for the determination of nitroxoline in medicines.
{"title":"Spectrophotometric Determination of Nitroxoline in Medicines Using the Response Surface Methodology","authors":"R. Bakeeva, S. Garmonov, V.D. Osipova, K.V. Chernyj, S.Yu. Mamykina, V. Sopin","doi":"10.26907/2542-064x.2023.1.118-132","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.118-132","url":null,"abstract":"It was shown by the conductometric study that the formation of micelles in the cetyltrimethylam¬monium bromide (CTAB) – dimethylsulfoxide (DMSO) – water system occurs at higher critical micelle concentrations (CMC) than in the CTAB – water system. The solubilization of nitroxoline in this system upon reaching the CMC was determined by the spectrophotometric method. The Box–Behnken design was used to obtain systems with the highest light absorption of nitroxoline, depending on the CTAB concentration, the acidity of the pH medium, and the proportion of DMSO when searching for the optimal matrix. A sensitive and selective technique suitable for micellar media and spectrophotometric analysis was developed using the response surface methodology for the determination of nitroxoline in medicines.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75621253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.2.175-189
G. Belyaev, A. Vyshtakalyuk, A. Parfenov, I. Galyametdinova, V. Semenov, V. Zobov
This article considers the antifibrotic properties of pyrimidine derivatives of the drug Xymedon (compound (I)) and its conjugate with L-ascorbic acid (compound (II)) in an experimental rat model of fibrosis with a preventive administration scheme. Experimental fibrosis was induced in Wistar rats given carbon tetrachloride (5% oil solution, 0.2 mL/kg orally twice a week) in combination with ethanol (5% solution in drinking water, free access) against compounds (I) and (II), both administered preventively. Fibrotic changes in the liver were detected by Van Gieson’s staining. The effects of the studied compounds on the liver and clinical condition of rats were evaluated through serum biochemical parameters. The treatment of rats with compounds (I) and (II) reduced the number of fibrotic areas threefold, ameliorated hepatic steatosis and necrosis as compared to the control group, and improved blood biochemical parameters (ALT, AST, and LDH). Interestingly, compound (II) had a more pronounced effect. Therefore, pyrimidine derivatives of Xymedon and its conjugate with L-ascorbic acid showed an antifibrotic effect in our experimental rat model of fibrosis.
{"title":"Antifibrotic Effect of Pyrimidine Derivatives of Xymedon and Its Conjugate with L-Ascorbic Acid","authors":"G. Belyaev, A. Vyshtakalyuk, A. Parfenov, I. Galyametdinova, V. Semenov, V. Zobov","doi":"10.26907/2542-064x.2023.2.175-189","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.2.175-189","url":null,"abstract":"This article considers the antifibrotic properties of pyrimidine derivatives of the drug Xymedon (compound (I)) and its conjugate with L-ascorbic acid (compound (II)) in an experimental rat model of fibrosis with a preventive administration scheme. Experimental fibrosis was induced in Wistar rats given carbon tetrachloride (5% oil solution, 0.2 mL/kg orally twice a week) in combination with ethanol (5% solution in drinking water, free access) against compounds (I) and (II), both administered preventively. Fibrotic changes in the liver were detected by Van Gieson’s staining. The effects of the studied compounds on the liver and clinical condition of rats were evaluated through serum biochemical parameters. The treatment of rats with compounds (I) and (II) reduced the number of fibrotic areas threefold, ameliorated hepatic steatosis and necrosis as compared to the control group, and improved blood biochemical parameters (ALT, AST, and LDH). Interestingly, compound (II) had a more pronounced effect. Therefore, pyrimidine derivatives of Xymedon and its conjugate with L-ascorbic acid showed an antifibrotic effect in our experimental rat model of fibrosis.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79848297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.5-22
R. Amirov, A. Solodov, R. M. Gataullina, J. R. Shaiymova, E. A. Burilova, A. Kiiamov, Y. Tyan, R. Batulin, M. Cherosov, D. Tayurskii
In this study, the method of thermal decomposition of iron(III) oleate at different ratios of oleic acid and octadecene-1 was used to obtain iron oxide nanoparticles (IONPs). Transmission electron microscopy (TEM) showed that they all had a spherical shape and a diameter of 8–9 nm, and FT-IR spectroscopy revealed the presence of oleate shells in them, which ensure the stability of the colloidal solutions of these nanoparticles in nonpolar solvents. For all synthesized IONPs, the sizes of the crystalline core formed by iron oxides were determined using X-ray diffraction analysis and magnetometry. The sizes of the oleate protective shells were established by comparison with the TEM data. These values were in agreement with those from the literature.
{"title":"Magnetic and Structural Properties of Iron Oxide Nanoparticles Produced by Thermal Decomposition of Precursors in Solution","authors":"R. Amirov, A. Solodov, R. M. Gataullina, J. R. Shaiymova, E. A. Burilova, A. Kiiamov, Y. Tyan, R. Batulin, M. Cherosov, D. Tayurskii","doi":"10.26907/2542-064x.2023.1.5-22","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.5-22","url":null,"abstract":"In this study, the method of thermal decomposition of iron(III) oleate at different ratios of oleic acid and octadecene-1 was used to obtain iron oxide nanoparticles (IONPs). Transmission electron microscopy (TEM) showed that they all had a spherical shape and a diameter of 8–9 nm, and FT-IR spectroscopy revealed the presence of oleate shells in them, which ensure the stability of the colloidal solutions of these nanoparticles in nonpolar solvents. For all synthesized IONPs, the sizes of the crystalline core formed by iron oxides were determined using X-ray diffraction analysis and magnetometry. The sizes of the oleate protective shells were established by comparison with the TEM data. These values were in agreement with those from the literature.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74420012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.37-48
A. S. Morozova, E. O. Kudryavtseva, S. Ziganshina, M. Ziganshin, A. Bukharaev
The mechanism of self-assembly by short-chain peptides (oligopeptides) – the process by which their molecules spontaneously form an ordered structure – has received much attention recently. Self-assembling phenylalanine oligopeptides have been of particular interest due to their potential as an effective aid in the design of new functional materials. This paper considers the results of an SPM study on the ability of L-alanyl-L-phenylalanine to self-assemble into a thin film under the action of methanol vapor. The micro- and nanostructures that develop on the surface of amorphous films of this dipeptide were characterized. A method for monitoring the state of the surface of dipeptide films using atomic force spectroscopy was proposed. The results obtained contribute to the development of approaches for the controlled self-assembly of oligopeptides used to produce new biocompatible materials and environmentally friendly micro- and nanodevices that would help solve various problems in the medical, environmental, and energy fields.
{"title":"Self-Assembly of the Dipeptide L-Alanyl-L-Phenylalanine under the Action of Methanol Vapor with the Formation of Micro- and Nanostructures","authors":"A. S. Morozova, E. O. Kudryavtseva, S. Ziganshina, M. Ziganshin, A. Bukharaev","doi":"10.26907/2542-064x.2023.1.37-48","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.37-48","url":null,"abstract":"The mechanism of self-assembly by short-chain peptides (oligopeptides) – the process by which their molecules spontaneously form an ordered structure – has received much attention recently. Self-assembling phenylalanine oligopeptides have been of particular interest due to their potential as an effective aid in the design of new functional materials. This paper considers the results of an SPM study on the ability of L-alanyl-L-phenylalanine to self-assemble into a thin film under the action of methanol vapor. The micro- and nanostructures that develop on the surface of amorphous films of this dipeptide were characterized. A method for monitoring the state of the surface of dipeptide films using atomic force spectroscopy was proposed. The results obtained contribute to the development of approaches for the controlled self-assembly of oligopeptides used to produce new biocompatible materials and environmentally friendly micro- and nanodevices that would help solve various problems in the medical, environmental, and energy fields.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78820690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.1.158-169
Спектр Ямр, Спектр Яmp
Alkylation reactions of α-carboxylate phosphabetaines were carried out and studied to enhance the biological activity of previously synthesized carboxylate phosphabetaines. As a result of these reactions, the original structure was destroyed with the formation of quaternary salts of phosphonium triiodide. The structure and composition were confirmed by a complex of physical research methods, including NMR, IR spectroscopy, and elemental analysis. The bactericidal and antimycotic activity of the synthesized salts was assessed. The compounds showed activity similar to that of commercial drugs. The reactions of complexation of these structures were also investigated. In the reactions with nickel and copper chloride, complexes were isolated and characterized. The structure of the nickel complex was unambiguously confirmed by the data obtained with the help of single-crystal X-ray diffraction analysis.
{"title":"α-Carboxylate Phosphabetains in Alkylation and Complexation Reactions","authors":"Спектр Ямр, Спектр Яmp","doi":"10.26907/2542-064x.2023.1.158-169","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.1.158-169","url":null,"abstract":"Alkylation reactions of α-carboxylate phosphabetaines were carried out and studied to enhance the biological activity of previously synthesized carboxylate phosphabetaines. As a result of these reactions, the original structure was destroyed with the formation of quaternary salts of phosphonium triiodide. The structure and composition were confirmed by a complex of physical research methods, including NMR, IR spectroscopy, and elemental analysis. The bactericidal and antimycotic activity of the synthesized salts was assessed. The compounds showed activity similar to that of commercial drugs. The reactions of complexation of these structures were also investigated. In the reactions with nickel and copper chloride, complexes were isolated and characterized. The structure of the nickel complex was unambiguously confirmed by the data obtained with the help of single-crystal X-ray diffraction analysis.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74561230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.2.216-230
A. Ratushnyy
Mesenchymal stromal cells (MSCs), a population of progenitor cells in adult tissues, are involved in the processes of physiological tissue renewal and damage-induced regeneration. MSCs have been widely studied as regenerative medicine agents. In this regard, the tissue-specific features of MSC subpopulations should be taken into account. MSCs have many potentially beneficial properties that can alter significantly with age. The ability of a cell to respond to external signals and regulate its functional state is commonly attributed to the repertoire of receptors on the cell membrane. This article considers the surface marker expression of senescent adipose-derived MSCs (AD-MSCs). Replicative senescence was caused by long-term cultivation. An increase in the expression of CD29, CD44, CD54, CD73, CD90, and HLA-ABC on the AD-MSCs was shown. The expression of CD105 and CD51/61 did not change reliably under the experimental conditions. The revealed effects are related not only to the larger cell size or higher autofluorescence, but also to the increased number of markers per unit area of the cell surface. The detected changes may underlie a number of modifications in the properties of senescent MSCs, including migration, adhesion, and immunomodulatory and angiogenic activities.
{"title":"Immunophenotype of Replicative Senescent Mesenchymal Stromal Cells","authors":"A. Ratushnyy","doi":"10.26907/2542-064x.2023.2.216-230","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.2.216-230","url":null,"abstract":"Mesenchymal stromal cells (MSCs), a population of progenitor cells in adult tissues, are involved in the processes of physiological tissue renewal and damage-induced regeneration. MSCs have been widely studied as regenerative medicine agents. In this regard, the tissue-specific features of MSC subpopulations should be taken into account. MSCs have many potentially beneficial properties that can alter significantly with age. The ability of a cell to respond to external signals and regulate its functional state is commonly attributed to the repertoire of receptors on the cell membrane. This article considers the surface marker expression of senescent adipose-derived MSCs (AD-MSCs). Replicative senescence was caused by long-term cultivation. An increase in the expression of CD29, CD44, CD54, CD73, CD90, and HLA-ABC on the AD-MSCs was shown. The expression of CD105 and CD51/61 did not change reliably under the experimental conditions. The revealed effects are related not only to the larger cell size or higher autofluorescence, but also to the increased number of markers per unit area of the cell surface. The detected changes may underlie a number of modifications in the properties of senescent MSCs, including migration, adhesion, and immunomodulatory and angiogenic activities.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76080135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.26907/2542-064x.2023.2.263-280
D. Svetasheva, V. O. Tatarnikov, M. Ocheretny, O.I. Bakun
In this study, variations in the levels of oil hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), which are persistent organic pollutants generated by human activities, were assessed in the bottom sediments of the Caspian Sea (Russia) sampled from 2012 to 2021. The samples were analyzed for their chemical composition. The concentrations of the identified compounds responsible for hydrocarbon pollution were established. The quantitative results of the industrial environmental monitoring in the Russian sector of the Caspian Sea for subsoil use were also reviewed and processed by standard statistical methods. It was revealed that ΣPAH concentrations in the Northern and Middle Caspian Sea regions vary from analytical zero to 186.7 μg/kg and from zero to 467.8 μg/kg, respectively. The scale of oil pollution in these two regions was found to be determined by the following PAHs: phenanthrene, acenaphthene, and naphthalene. The origin of the listed PAHs provides vital information on the main sources of pollution of the Caspian Sea bottom sediments with hazardous organic substances. Based on the obtained data, the areas with background PAH pollution of the bottom sediments and those with the characteristic PAH of mainly natural and pyrogenic origin were located.
{"title":"Polyaromatic Hydrocarbons in the Bottom Sediments of the Caspian Sea","authors":"D. Svetasheva, V. O. Tatarnikov, M. Ocheretny, O.I. Bakun","doi":"10.26907/2542-064x.2023.2.263-280","DOIUrl":"https://doi.org/10.26907/2542-064x.2023.2.263-280","url":null,"abstract":"In this study, variations in the levels of oil hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), which are persistent organic pollutants generated by human activities, were assessed in the bottom sediments of the Caspian Sea (Russia) sampled from 2012 to 2021. The samples were analyzed for their chemical composition. The concentrations of the identified compounds responsible for hydrocarbon pollution were established. The quantitative results of the industrial environmental monitoring in the Russian sector of the Caspian Sea for subsoil use were also reviewed and processed by standard statistical methods. It was revealed that ΣPAH concentrations in the Northern and Middle Caspian Sea regions vary from analytical zero to 186.7 μg/kg and from zero to 467.8 μg/kg, respectively. The scale of oil pollution in these two regions was found to be determined by the following PAHs: phenanthrene, acenaphthene, and naphthalene. The origin of the listed PAHs provides vital information on the main sources of pollution of the Caspian Sea bottom sediments with hazardous organic substances. Based on the obtained data, the areas with background PAH pollution of the bottom sediments and those with the characteristic PAH of mainly natural and pyrogenic origin were located.","PeriodicalId":23418,"journal":{"name":"Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90699077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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