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Morphological and histopathological changes of maternal levetiracetam on the cerebellar cortex of the offspring of albino rat. 母体左乙拉西坦对白化大鼠后代小脑皮层的形态学和组织病理学变化
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-06-08 DOI: 10.1080/01913123.2024.2353064
Safaa M Hanafy

Levetiracetam (LEV) is being used by women with reproductive-age epilepsy at a significantly higher rate. The purpose of the study was to assess how levetiracetam treatment during pregnancy affected the offspring's weight and cerebellum. Forty pregnant rats were divided into two groups (I, II). Two smaller groups (A, B) were created from each group. The rats in group I were gavaged with approximately 1.5 mL/day of distilled water either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). The rats in group II were gavaged with about 1.5 mL/day of distilled water (containing 36 mg levetiracetam) either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). After the work was completed, the body weight of the pups in each group was recorded, and their cerebella were analyzed histologically and morphometrically. Following levetiracetam treatment, the offspring showed decreased body weight and their cerebella displayed delayed development and pathological alterations. These alterations manifested as, differences in the thicknesses of the layers of cerebellar cortex as compared to the control groups; additionally, their cells displayed cytoplasmic vacuolation, nuclear alterations, fragmented rough endoplasmic reticulum and lost mitochondrial cristae. Giving levetiracetam to pregnant and lactating female rats had a negative impact on the body weight and cerebella of the offspring. Levetiracetam should be given with caution during pregnancy and lactation.

育龄女性癫痫患者使用左乙拉西坦(LEV)的比例明显增加。这项研究的目的是评估怀孕期间服用左乙拉西坦对后代体重和小脑的影响。40 只怀孕大鼠被分为两组(I、II)。每组再分成两个小组(A、B)。I 组大鼠在怀孕期间(IA 亚组)或在怀孕期间和产后 14 天(IB 亚组)连续每天灌胃约 1.5 毫升蒸馏水。第二组大鼠在怀孕期间(IA 亚组)或怀孕期间和产后 14 天(IB 亚组)连续每天灌胃约 1.5 毫升蒸馏水(含 36 毫克左乙拉西坦)。工作结束后,记录各组幼崽的体重,并对其大脑进行组织学和形态学分析。在接受左乙拉西坦治疗后,后代的体重下降,大脑发育迟缓并出现病理改变。与对照组相比,这些改变表现为小脑皮质各层厚度的差异;此外,它们的细胞还表现出细胞质空泡化、核改变、粗面内质网破碎和线粒体嵴消失。给怀孕和哺乳期雌性大鼠服用左乙拉西坦对后代的体重和小脑有负面影响。妊娠期和哺乳期应慎用左乙拉西坦。
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引用次数: 0
Infection as a trigger of acute, transient glomerular deposition of clonal immunoglobulins. 感染是克隆免疫球蛋白急性、一过性肾小球沉积的诱因。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-05-16 DOI: 10.1080/01913123.2024.2356112
Bangchen Wang, Micah Schub, Derrick L Robinson, David N Howell

Glomerular deposition of monoclonal IgM, frequently in the form of intracapillary pseudothrombi, can be seen in Waldenström macroglobulinemia (WM) and type I cryoglobulinemia (CG). They are typically associated with plasma cell or B-lymphoid neoplasms, particularly lymphoplasmacytic lymphoma (LPL). While infection is a frequent trigger of mixed (type II and III) CG, its association with type I CG is uncommon. We report two cases in which striking lambda-chain-restricted IgM deposits and acute kidney injury (AKI) occurred in the setting of known or suspected systemic infections, with prompt resolution on treatment of the infection.

在瓦尔登斯特伦巨球蛋白血症(WM)和Ⅰ型低温球蛋白血症(CG)中可见到单克隆 IgM 在肾小球沉积,经常表现为毛细血管内假血栓。它们通常与浆细胞或 B 淋巴肿瘤有关,尤其是淋巴浆细胞性淋巴瘤(LPL)。虽然感染是混合型(II 型和 III 型)CG 的常见诱因,但感染与 I 型 CG 的关联并不常见。我们报告了两个病例,这两个病例在已知或疑似全身感染的情况下出现了惊人的λ-链限制性 IgM 沉积和急性肾损伤(AKI),并在治疗感染后迅速缓解。
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引用次数: 0
Hydralazine use can be associated with IgM-dominated immune complex-mediated glomerulonephritis. 使用氯丙嗪可能与 IgM 主导的免疫复合物介导的肾小球肾炎有关。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-04-29 DOI: 10.1080/01913123.2024.2346660
Ping L Zhang, Brandon D Metcalf, Sarang Khan, Jamal Abukhaled, Khalid Zafar, Wei Li, Hassan D Kanaan

Context: IgM-dominant immune complex-mediated glomerulonephritis (IgM-dominant ICMGN) is a rare renal entity, characterized by a membranoproliferative pattern by light microscopy, dominant IgM staining by immunofluorescent staining, and subendothelial deposits by electron microscopy. This study was to investigate if some of IgM-ICMGN were associated with autoimmune disorders induced by hydralazine.

Design: Seven IgM-dominant ICMGN cases were identified over 8 years. Their pathologic phenotypes and clinical scenarios were analyzed in detail.

Results: Patients' ages ranged from 47 to 87 years old with 5 women and two men. Six of seven patients had drug-induced autoimmune phenomenon (hydralazine-induced positive ANCA and ANA). All of them had renal dysfunction and some proteinuria. Most pathologic features showed a membranoproliferative pattern of glomerulonephritis with dominant IgM deposits at subendothelial spaces. IgM nephropathy (a variant of focal segmental glomerulosclerosis), chronic thrombotic microangiopathy, and cryoglobulinemic glomerulopathy were ruled out in the cases.

Conclusion: The hydralazine-induced autoimmune phenomenon can be seen in IgM-dominant ICMGN, which should be classified as a subtype of membranoproliferative glomerulonephritis.

背景:IgM显性免疫复合物介导的肾小球肾炎(IgM-dominant ICMGN)是一种罕见的肾脏疾病,其特点是光镜下为膜增生型,免疫荧光染色为显性IgM染色,电镜下为内皮下沉积。本研究旨在探讨某些 IgM-ICMGN 是否与水拉嗪诱发的自身免疫性疾病有关:设计:历时 8 年,共发现 7 例 IgM-ICMGN 病例。详细分析了他们的病理表型和临床表现:患者年龄从 47 岁到 87 岁不等,其中女性 5 人,男性 2 人。七名患者中有六名出现了药物诱导的自身免疫现象(水拉嗪诱导的 ANCA 和 ANA 阳性)。所有患者都有肾功能障碍和一些蛋白尿。大多数病理特征显示肾小球肾炎呈膜增生型,内皮下间隙有明显的 IgM 沉积。这些病例排除了 IgM 肾病(局灶节段性肾小球硬化症的一种变异)、慢性血栓性微血管病和冷球蛋白血症肾小球病的可能性:结论:氢氯嗪诱导的自身免疫现象可见于 IgM 主导型 ICMGN,应将其归类为膜增生性肾小球肾炎的一种亚型。
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引用次数: 0
Chronic liver fibrosis induction in aging causes significant ultra-structural deterioration in liver and alteration on immune response gene expressions in liver-spleen axis. 衰老诱导的慢性肝纤维化会导致肝脏超结构显著恶化,并改变肝脾轴心的免疫反应基因表达。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-06-06 DOI: 10.1080/01913123.2024.2360447
Zeynal Mete Karaca, Gamze Karaca, Başak Kayhan, Mehmet Gül, Veysel Ersan, Harika Gözükara Bağ, Elif Yeşilada

The relationship between damage to the liver and spleen by aging and the immune response status in these two organs, which are anatomically and immunologically interconnected, is unknown. The authors investigated the histopathological, ultrastructural, and immunological effects of aging in young and aged fibrotic mice by using an experimental model. Four groups were planned, with 10 mice in each experimental group. The levels of fibrosis and ultrastructural destruction in the liver were determined by α-SMA staining and TEM analysis. Expression levels of immunity genes (Il2, Il4, Il6, Il10, Il12, Il17, Tnf, Ifng, Tgfb1, Gata3, Rorc, Tbx21, Foxp3, Ccl2, Ccr2, Cxcr3, Pf4, Cxcl10) were carried out by qRT-PCR. While structural disorders were detected in the mitochondria of aged healthy group, cellular destruction in the fibrosis-induced elderly group was at a dramatic level. Fibrosis induction in aged mice caused an elevation in the expression of chemokines (CCl2, CXCL10, CCR2) and cytokine (IL-17a) genes that induce autoinflammatory response in the liver. Unlike the cellular pathology and genes activated in fibrosis in youth and the natural occurrence of fibrosis with aging, induction of fibrosis during aging causes deterioration in the liver and expression of genes responsible for autoimmunity in both the liver and spleen.

肝脏和脾脏在解剖学和免疫学上相互关联,而衰老对肝脏和脾脏的损伤与这两个器官的免疫反应状态之间的关系尚不清楚。作者通过实验模型研究了衰老对年轻和老年纤维化小鼠的组织病理学、超微结构和免疫学影响。实验计划分为四组,每组 10 只小鼠。通过α-SMA染色和TEM分析确定肝脏纤维化和超微结构破坏的程度。免疫基因(Il2、Il4、Il6、Il10、Il12、Il17、Tnf、Ifng、Tgfb1、Gata3、Rorc、Tbx21、Foxp3、Ccl2、Ccr2、Cxcr3、Pf4、Cxcl10)的表达水平通过 qRT-PCR 进行检测。虽然在健康老年组的线粒体中检测到了结构紊乱,但在纤维化诱导的老年组中,细胞破坏却达到了惊人的程度。诱导老年小鼠纤维化会导致趋化因子(CCl2、CXCL10、CCR2)和细胞因子(IL-17a)基因的表达升高,从而诱发肝脏的自身炎症反应。与年轻时纤维化的细胞病理学和激活的基因以及随着年龄增长而自然发生的纤维化不同,衰老过程中纤维化的诱导会导致肝脏恶化以及肝脏和脾脏中负责自身免疫的基因的表达。
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引用次数: 0
The potential therapeutic effects of exosomes derived from bone marrow mesenchymal stem cells on ileum injury of a rat sepsis model (histological and immunohistochemical study). 骨髓间充质干细胞外泌体对败血症模型大鼠回肠损伤的潜在治疗作用(组织学和免疫组化研究)。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-07-01 DOI: 10.1080/01913123.2024.2368011
Heba M Elnegris, Abeer A Abdelrahman, Eman S El-Roghy

Sepsis denotes a serious high mortality concern. The study was designed to evaluate the effect of mesenchymal stem cell exosomes (MSC-exosomes) on the evolution of the animal model of sepsis. In this study, 36 rats were distributed into three groups, (I) controls, (II) LPS-treated, and (III) LPS+MSC-EVs. Sepsis was simulated by administering E. coli-LPS to the laboratory animals. Group III was given MSC-exosomes four hours after the LPS injection. Forty-eight hours later rats were sacrificed. Ileum samples were excised, and processed for the histological assessment, immunohistochemical identification of CD44, and inducible nitric oxide synthase (iNOS). Ileum homogenate was used to estimate tumor necrosis factor α (TNF α) besides Cyclooxygenase-2 (COX 2). PCR was used for the detection of interleukin 1α (IL‑1α), and interleukin 17 (IL‑17). Statistical and morphometrical analysis was done. The LPS-treated group showed increased TNF-α, IL‑1α, IL‑17, and decreased COX 2. LPS administration led to cytoplasmic vacuolization of enterocytes, an increase in the vasculature, and cellular infiltrations invaded the lamina propria. There was a significant rise in goblet cells and the proportion of collagen fibers. Ultrastructurally, the enterocytes displayed nuclear irregularity, rough endoplasmic reticulum (rER) dilatation, and increased mitochondria number. Sepsis induces a significant increase in iNOS and a decrease in CD44 immune expressions. LPS+MSC-EVs group restored normal ileum structure and revealed a significant elevation in CD44 and a reduction in iNOS immunoreactions. LPS-sepsis induced an obvious ileum inflammatory deterioration ameliorated by MSC-exosomes, mostly through their antioxidant, anti-inflammatory, and anti-apoptotic properties.

败血症是一种严重的高死亡率疾病。本研究旨在评估间充质干细胞外泌体(MSC-exosomes)对败血症动物模型演变的影响。在这项研究中,36只大鼠被分为三组,(I) 对照组,(II) LPS处理组,(III) LPS+间充质干细胞外泌体组。通过给实验动物注射大肠杆菌-LPS来模拟败血症。在注射 LPS 四小时后,给第三组大鼠注射间充质干细胞外泌体。48 小时后,大鼠被处死。切除回肠样本并进行组织学评估、CD44和诱导型一氧化氮合酶(iNOS)的免疫组化鉴定。回肠匀浆用于评估肿瘤坏死因子α(TNF α)和环氧化酶-2(COX 2)。白细胞介素 1α(IL-1α)和白细胞介素 17(IL-17)的检测采用 PCR 技术。进行了统计和形态学分析。LPS 处理组显示 TNF-α、IL-1α、IL-17 增加,COX 2 减少。给予 LPS 会导致肠细胞胞浆空泡化,血管增加,细胞浸润侵入固有层。鹅口疮细胞和胶原纤维的比例明显上升。从超微结构上看,肠细胞核不规则,粗面内质网(rER)扩张,线粒体数量增加。败血症会诱导 iNOS 的显著增加和 CD44 免疫表达的减少。LPS+MSC-EVs 组恢复了正常的回肠结构,并显示出 CD44 的显著升高和 iNOS 免疫反应的降低。间充质干细胞外泌体主要通过其抗氧化、抗炎和抗凋亡特性改善了LPS败血症引起的回肠炎症恶化。
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引用次数: 0
Unveiling renal pathology's potential: exploring a rare subtype of amyloid - apolipoprotein CII amyloidosis in the youngest patient: a case report and literature review. 揭示肾脏病理学的潜力:探索一种罕见的淀粉样蛋白亚型--最年轻患者的载脂蛋白CII淀粉样变性:病例报告和文献综述。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-05-20 DOI: 10.1080/01913123.2024.2353397
Yiqin Zuo, Fiona Hanly, Duo Li, Efren Chavez, Omar Aljuboori, Gabriel Contreras, Guillermo A Herrera

In this clinical case report, we present a rare subtype of amyloidosis, apolipoprotein CII (apo CII), which was diagnosed through a renal biopsy and subsequently confirmed by identifying the p.K41T mutation via germline DNA sequencing. Upon reviewing the literature, five patients exhibiting identical mutation were identified via renal biopsy, while an additional patient was diagnosed through biopsies of the fat pad and bone marrow. Notably, our patient is the youngest recorded case. We pioneered the application of immunofluorescence and immunogold electron microscopy techniques for apo CII evaluation. Our report provides a detailed description of this case, supplemented by an extensive review encompassing apo CII, documented instances of apo CII amyloidosis with renal or systemic involvement, and potential underlying mechanisms.

在这份临床病例报告中,我们介绍了一种罕见的亚型淀粉样变性病--载脂蛋白 CII(载脂蛋白 CII),该病是通过肾活检确诊的,随后通过种系 DNA 测序确定了 p.K41T 突变。查阅文献后发现,有五名患者通过肾活检发现了相同的突变,另有一名患者是通过脂肪垫和骨髓活检确诊的。值得注意的是,我们的患者是有记录以来最年轻的病例。我们率先将免疫荧光和免疫金电子显微镜技术应用于载脂蛋白 CII 的评估。我们的报告对该病例进行了详细描述,并对载脂蛋白 CII、载脂蛋白 CII 淀粉样变性累及肾脏或全身的记录病例以及潜在的潜在机制进行了广泛综述。
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引用次数: 0
Effect of peripheral blood mononuclear cells on ischemia-reperfusion injury of sciatic nerve of adult male albino rat: histological, immunohistochemical, and ultrastructural study. 外周血单核细胞对成年雄性白化大鼠坐骨神经缺血再灌注损伤的影响:组织学、免疫组织化学和超微结构研究。
IF 1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-05-03 Epub Date: 2024-02-29 DOI: 10.1080/01913123.2024.2321144
Shaimaa Mostafa Kashef, Suzan Elsayed Abo Elnasr

Ischemia/reperfusion (I/R) injury of sciatic nerve is a serious condition that results in nerve fiber degeneration, and reperfusion causes oxidative injury. Peripheral blood mononuclear cells (PBMNCs) have neuroregenerative power. This study was carried out to evaluate the potential ameliorative effect of PBMNCs on changes induced by I/R injury of the sciatic nerve. Fifty adult male albino rats were divided into donor and experimental groups that were subdivided into four groups: group I (control group), group II received 50 µL PBNMCs once intravenously via the tail vein, group III rubber tourniquet was placed around their Rt hind limb root for 2 hours to cause ischemia, group IV was subjected to limb ischemia as group III, then they were injected with 50 ul PBMNCs as group II before reperfusion. I/R injury showed disorganization of nerve fascicles with wide spaces in between nerve fibers. The mean area of collagen fibers, iNOS immunoexpression, and number of GFAP-positive Schwann cells of myelinated fibers showed a highly significant increase, while a highly significant reduction in the G-ratio and neurofilament immunoexpression was observed. Myelin splitting, invagination, evagination, and myelin figures were detected. PBMNC-treated group showed a marked improvement that was confirmed by histological, immunohistochemical, and ultrastructural findings.

坐骨神经缺血再灌注(I/R)损伤是一种导致神经纤维变性的严重疾病,再灌注会造成氧化损伤。外周血单核细胞(PBMNCs)具有神经再生能力。本研究旨在评估 PBMNCs 对坐骨神经 I/R 损伤引起的变化的潜在改善作用。将 50 只成年雄性白化大鼠分为供体组和实验组,实验组又分为四组:I 组(对照组)、II 组经尾静脉注射一次 50 µL PBNMCs、III 组在其右后肢根部缠绕橡胶止血带 2 小时以造成缺血、IV 组与 III 组一样进行肢体缺血,然后与 II 组一样在再灌注前注射 50 ul PBMNCs。I/R损伤表现为神经纤维束紊乱,神经纤维之间的空隙变大。有髓鞘纤维的胶原纤维平均面积、iNOS 免疫表达和 GFAP 阳性许旺细胞的数量均有非常显著的增加,而 G 比率和神经丝蛋白免疫表达则有非常显著的减少。检测到髓鞘分裂、内陷、剥离和髓鞘图形。PBMNC 治疗组的病情明显好转,组织学、免疫组化和超微结构研究结果均证实了这一点。
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引用次数: 0
Ultrastructural differences between synovial sarcoma and solitary fibrous tumor: comparative study in adult patients from the National Cancer Institute of Mexico. 滑膜肉瘤与单发纤维瘤的超微结构差异:墨西哥国家癌症研究所成年患者的比较研究。
IF 1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-05-03 Epub Date: 2024-02-13 DOI: 10.1080/01913123.2024.2313742
Guillermo Ernesto Corredor-Alonso, Leonardo Saúl Lino-Silva, Erika Yazmin López-Flores, Tatiana Velásquez-Tovar, Hugo Ricardo Domínguez-Malagón

Synovial sarcoma (SS) and solitary fibrous tumor (SFT) are entities with considerable morphological and immunohistochemical similarities that sometimes show a non-confirmatory profile (TLE1 negative, CD34 and focal or negative STAT6 and lack of specific fusion IHC markers), in which the utility ultrastructure is unknown. A cross-sectional, retrospective, analytical, nonexperimental study was carried out by the Department of Pathology of the National Cancer Institute of Mexico (INCan) e from January 1, 2009 to December 31, 2018. With 17 SFT cases with diffuse or focal CD34 and STAT6 positivity and 18 cases of SS with positive FISH molecular test t(X:18) breakapart were studied by electron microscopy of fresh glutaraldehyde fixed or paraffin-embedded tissue. The ultrastructural findings with a significant difference present in the SS were tandem tight junctions, desmosomes and abundance of dilated rough endoplasmic reticulum (RER) cisternae (p < 0.001, 0.003, and 0.001, respectively); while in the (SFT) the presence of abundant glycogen, basal lamina, long and slender cytoplasmic processes, pinocytic vesicles, hemidesmosomes, and/or dense plaques, collagen skein, and microvilli-like buds (p = 0.028, 0.005, and <0.001 for the last five). We then infer that the five distinctive markers of the SFT are the collagen skeins intermingled with cellular processes in a shape of "squid can," and the pinocytic vesicles as they were not observed in any case of SS. Conversely, tandem junctions were not found in any SFT case. Although the presence of multivesicular buds in the SFT was not significant, it had not been previously described.

滑膜肉瘤(SS)和单发纤维瘤(SFT)在形态学和免疫组化方面具有相当大的相似性,有时表现为非确证性特征(TLE1阴性、CD34和STAT6灶性或阴性,缺乏特异性融合IHC标记物),其中的实用超微结构尚不清楚。墨西哥国家癌症研究所(INCan)病理学部于 2009 年 1 月 1 日至 2018 年 12 月 31 日开展了一项横断面、回顾性、分析性、非实验性研究。通过对新鲜戊二醛固定或石蜡包埋组织进行电镜观察,研究了17例CD34和STAT6弥漫或局灶阳性的SFT病例和18例FISH分子检测t(X:18)断裂阳性的SS病例。在 SS 中存在显著差异的超微结构发现是串联紧密连接、脱钙小体和大量扩张的粗面内质网(RER)贮液器(p p = 0.028、0.005 和 0.006)。
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引用次数: 0
Robert Erlandson obituary (September 4, 1937-February 4, 2024): a true giant and great champion of ultrastructural pathology. 罗伯特-厄兰森(Robert Erlandson)(1937 年 9 月 4 日-2024 年 2 月 4 日):超微结构病理学的真正巨人和伟大倡导者。
IF 1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-05-03 Epub Date: 2024-03-31 DOI: 10.1080/01913123.2024.2334996
David M Parham
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引用次数: 0
Effect of platelet rich plasma versus melatonin on testicular injury induced by Busulfan in adult albino rats: a histological and immunohistochemical study. 富血小板血浆和褪黑素对成年白化大鼠布舒凡所致睾丸损伤的影响:组织学和免疫组化研究。
IF 1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-05-03 Epub Date: 2024-02-29 DOI: 10.1080/01913123.2024.2322567
Eman M Askar, Amira M Abdelmegid, Laila M Elshal, Mohamed A Shaheen

This study was done to estimate the testicular histological alterations induced by Busulfan (BUS) and compare the possible protective effects of melatonin (MT) and platelet rich plasma (PRP) in a rat model. Sixty-four male rats were dispersed into: control group, BUS group, melatonin group, and PRP group. Blood samples were processed for biochemical analysis. Tissue specimens were managed for light and electron microscopic studies. Immunohistochemical expression of vimentin and proliferating cell nuclear antigen (PCNA) was performed. Busulfan induced severe testicular damage in all studied methodologies. It showed a statistically significant decrease in serum testosterone and elevation of MDA when compared to the control group. Abnormal testicular cytostructures suggesting defective spermatogenesis were observed: distorted seminiferous tubules, deformed spermatogenic cells, low germinal epithelium height, few mature spermatozoa, and also deformed barrier. Vimentin and PCNA expressions were reduced. Ultrastructurally, Sertoli cells and the blood testis barrier were deformed, spermatogenic cells were affected, and mature spermatozoa were few and showed abnormal structure. Both melatonin and PRP induced improvement in all the previous parameters and restoration of spermatogenesis as confirmed by improvement of Johnsen's score from 2.6 ± .74 to 7.6 ± .92. In conclusion, melatonin and PRP have equal potential to ameliorate the testicular toxicity of BUS. Melatonin can provide a better noninvasive way to combat BUS induced testicular injury.

本研究旨在评估布舒凡(BUS)诱导的睾丸组织学改变,并比较褪黑素(MT)和富血小板血浆(PRP)对大鼠模型可能产生的保护作用。64 只雄性大鼠被分为:对照组、BUS 组、褪黑素组和 PRP 组。对血液样本进行生化分析。对组织标本进行光镜和电子显微镜研究。对波形蛋白和增殖细胞核抗原(PCNA)进行免疫组化表达。在所有研究方法中,布舒凡均诱发了严重的睾丸损伤。与对照组相比,血清睾酮的下降和MDA的升高具有统计学意义。观察到的异常睾丸细胞结构表明精子发生存在缺陷:曲细精管扭曲、生精细胞变形、生精上皮高度低、成熟精子少以及屏障变形。波形蛋白和 PCNA 表达减少。超微结构上,Sertoli细胞和睾丸血屏障变形,生精细胞受到影响,成熟精子数量少且结构异常。褪黑素和睾丸生精细胞生长因子(PRP)均能改善之前的所有参数,恢复精子生成,Johnsen评分从2.6 ± .74提高到7.6 ± .92,证实了这一点。总之,褪黑素和 PRP 在改善 BUS 的睾丸毒性方面具有同等潜力。褪黑激素可以提供一种更好的非侵入性方法来对抗 BUS 引起的睾丸损伤。
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引用次数: 0
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Ultrastructural Pathology
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