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The possible protective effect of ginger extract on toxic changes induced by bisphenol A on the thyroid gland of adult male albino rats: light and electron microscopic study. 生姜提取物对双酚 A 诱导的成年雄性白化大鼠甲状腺毒性变化的可能保护作用:光镜和电子显微镜研究。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-11-01 Epub Date: 2024-08-27 DOI: 10.1080/01913123.2024.2395849
Sara Mohammed Taha Denewer, Safinaz Moustafa Mahmoud Ali, Nawal Awad Hasanin, Dalia Refat El-Bassouny

Bisphenol A (BPA) is a chemical substance used in the plastic industry and considered as an endocrine disruptor. Ginger is a herbal material used in the food industry and has antioxidant activity. The present study was performed to evaluate the histological changes in the thyroid gland of adult male albino rats after intake of BPA and if there is any protective role for ginger extract (GE). Eighty adult male rats were divided equally into four groups. Group I as a control group, group II included rats that received 250 mg/kg/day GE orally for eight weeks, group III included rats that received 200 mg/kg/day BPA orally for the same period and group IV included rats that received BPA in the same dose for the same duration concomitantly with GE. At the end of the experiment, blood samples were taken for hormonal essay and tissue samples were processed. Light and electron microscopic studies were done. Morphometric and statistical studies were carried out. Group III showed degenerative changes in the thyroid gland, decreased serum levels of T3 and T4 and a strong positive inducible nitric oxide synthase (iNOS) immune response. Group IV showed restoration of thyroid gland architecture and function. In conclusion, GE protected the thyroid structure from the damaging effect of BPA oxidative stress through its anti-oxidant effect, thus preserving thyroid activity.

双酚 A(BPA)是一种用于塑料工业的化学物质,被认为是一种内分泌干扰物。生姜是一种用于食品工业的草药,具有抗氧化活性。本研究旨在评估成年雄性白化大鼠摄入双酚 A 后甲状腺的组织学变化,以及生姜提取物(GE)是否具有保护作用。研究人员将 80 只成年雄性大鼠平均分为四组。第一组为对照组,第二组大鼠每天口服 250 毫克/千克生姜提取物,连续八周;第三组大鼠每天口服 200 毫克/千克双酚 A,连续八周;第四组大鼠在口服相同剂量双酚 A 的同时口服生姜提取物,连续八周。实验结束后,对大鼠的血液样本进行激素分析,并对组织样本进行处理。进行了光镜和电子显微镜研究。进行了形态计量学和统计学研究。第三组显示甲状腺发生了退行性变化,血清中的T3和T4水平下降,诱导型一氧化氮合酶(iNOS)免疫反应呈强阳性。第四组则显示出甲状腺结构和功能的恢复。总之,通用电气通过抗氧化作用保护甲状腺结构免受双酚 A 氧化应激的破坏,从而保持甲状腺的活性。
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引用次数: 0
Value of electron microscopy as perceived by nephrologists in Flanders, Belgium. 比利时佛兰德斯地区肾病专家对电子显微镜价值的看法。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-11-01 Epub Date: 2024-11-04 DOI: 10.1080/01913123.2024.2423083
Laura Desnerck, Laure-Anne Kerckhaert, Steven Van Laecke, Amélie Dendooven

Electron microscopy (EM) is an important complementary tool in biopsy diagnosis of kidney disease. However, EM is a costly technique and not universally available. In order to understand nephrologists' perspectives on EM, a survey among Flemish nephrologists was conducted. The survey explores nephrologists' knowledge and satisfaction with EM, the barriers in its use, and its role in decision-making.A questionnaire was sent out to Dutch-speaking nephrologists in Belgium (Flanders) via the professional organization NBVN (Nederlandstalige Belgische Vereniging voor Nefrologie).The average satisfaction of EM accessibility in nephrologists, was 4.0 on a scale from 1 (very unsatisfied) to 5 (very satisfied). The main barrier in ordering EM appeared to be the long turnaround time, indicated by 32.5% of nephrologists. The reports were found mostly understandable by 61.0% of the nephrologists. The impact of EM on diagnosis of kidney disease was estimated higher than its impact on the treatment: 24.4% of respondents estimated diagnosis changes in less than 5% of cases, versus 68.3% estimated treatment changes in less than 5% of cases.This study provides key insights into nephrologists' perception on EM services, revealing high overall satisfaction. However, there is potential for improvement, especially regarding turnaround times.

电子显微镜(EM)是活检诊断肾脏疾病的重要辅助工具。然而,电子显微镜是一种昂贵的技术,并非普遍可用。为了了解肾脏病专家对 EM 的看法,我们对佛兰德的肾脏病专家进行了一项调查。通过专业组织 NBVN(Nederlandstalige Belgische Vereniging voor Nefrologie)向比利时(佛兰德斯)讲荷兰语的肾病学家发放了调查问卷。肾病学家对 EM 可及性的平均满意度为 4.0,满意度从 1(非常不满意)到 5(非常满意)不等。32.5%的肾科医生表示,订购电子病历的主要障碍似乎是周转时间过长。61.0%的肾病学专家认为这些报告大多可以理解。据估计,EM 对肾脏疾病诊断的影响高于对治疗的影响:24.4% 的受访者估计,在不到 5% 的病例中,诊断发生了变化,而 68.3% 的受访者估计,在不到 5% 的病例中,治疗发生了变化。然而,仍有改进的余地,尤其是在周转时间方面。
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引用次数: 0
Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects. 由甲萘醌:抗坏血酸盐组合诱导的核酸酶再激活与过氧化损伤对人类前列腺癌的破坏:超微结构方面。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1080/01913123.2024.2379300
Jacques Gilloteaux, James M Jamison, Jack L Summers, Henryk S Taper

Introduction: Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after a prooxidant anticancer treatment consisting of a combination of menadione bisulfite and sodium ascorbate (VK3:VC).

Methods: Implanted samples of diaphragm carcinomas from longest survived mice from either oral, intraperitoneal (IP), or both oral and IP treatment groups were assessed with light, scanning, and transmission electron microscopy to analyze morphologic damages.

Results: Compared with previous fine structure data of in vitro untreated carcinomas, the changes induced by oral, IP, and oral with IP VK3:VC treatment dismantled those xenografts with autoschizis, and necrotic atrophy was accomplished by cell's oxidative stress whose injuries were consequent to reactivated deoxyribonucleases and ribonucleases. Tumor destructions resulted from irreversible damages of nucleus components, endoplasmic reticulum, and mitochondria there. Other alterations included those of the cytoskeleton that resulted in characteristic self-excisions named " autoschizis." All these injuries lead resilient cancer cells to necrotic cell death.

Conclusion: The fine structure damages caused by VK3:VC prooxidant combination in the human DU145 prostate xenografts confirmed those shown in vitro and of other cell lines with histochemistry and biomolecular investigations. These devastations incurred without damage to normal tissues; thus, our data brought support for the above combination to assist in the treatment of prostate cancers and other cancers.

导言:将雄激素依赖性人DU145前列腺转移癌的异种移植物植入nu/nu雄性小鼠体内,结果显示,经过由亚硫酸氢钠和抗坏血酸钠(VK3:VC)组合而成的促氧化剂抗癌治疗后,小鼠存活率显著提高:方法:用光镜、扫描显微镜和透射电子显微镜对口服组、腹腔注射组或口服组和腹腔注射组存活时间最长的小鼠的膈肌癌植入样本进行评估,分析其形态损伤情况:结果:与之前未经处理的体外癌的精细结构数据相比,口服、腹腔注射和口服加腹腔注射VK3:VC治疗诱发的变化使这些异种移植物自体分裂,坏死萎缩是由细胞的氧化应激完成的,其损伤是脱氧核糖核酸酶和核糖核酸酶重新激活的结果。细胞核成分、内质网和线粒体的不可逆损伤导致了肿瘤的破坏。其他改变还包括细胞骨架的改变,这些改变导致了名为 "自体分裂 "的特征性自我分裂。所有这些损伤都会导致顽强的癌细胞坏死:结论:VK3:VC 促氧化剂组合在人类 DU145 前列腺异种移植物中造成的精细结构破坏,证实了组织化学和生物分子研究在体外和其他细胞系中显示的破坏。这些破坏不会对正常组织造成损害;因此,我们的数据为上述组合辅助治疗前列腺癌和其他癌症提供了支持。
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引用次数: 0
The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line: an ultrastructural study. 替莫唑胺和伊瑞布林联合用药对 T98G 人胶质母细胞瘤细胞系的影响:超微结构研究。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-06-25 DOI: 10.1080/01913123.2024.2371821
Gamze Tanriverdi, Belisa Kaleci, Furkan Yavuz, Hakan Sahin, Merjem Purelku, Zeliha Yazici, Sibel Kokturk

Glioblastoma tumors are the most aggressive primary brain tumors that develop resistance to temozolomide (TMZ). Eribulin (ERB) exhibits a unique mechanism of action by inhibiting microtubule dynamics during the G2/M cell cycle phase. We utilized the T98G human glioma cell line to investigate the effects of ERB and TMZ, both individually and in combination. The experimental groups were established as follows: control, E5 (5 nM ERB), T0.75 (0.75 mM TMZ), T1 (1.0 mM TMZ), and combination groups (E5+T0.75 and E5+T1). All groups showed a significant decrease in cell proliferation. Apoptotic markers revealed a time-dependent increase in annexin-V expression, across all treatment groups at the 48-hour time point. Caspase-3, exhibited an increase in the combination treatment groups at the 48-hour mark. Transmission electron microscopy (TEM) revealed normal ultrastructural features in the glioma cells of the control group. However, treatments induced ultrastructural changes within the spheroid glioblastoma model, particularly in the combination groups. These changes included a dose-dependent increase in autophagic vacuoles and apoptotic morphology of the cells. In conclusion, the similarity in the mechanism of action between ERB and TMZ suggests the potential for synergistic effects when combined. Our results highlight that this combination induced severe damage and autophagy in glioma spheroids after 48 hours.

胶质母细胞瘤是最具侵袭性的原发性脑肿瘤,会对替莫唑胺(TMZ)产生抗药性。艾瑞布林(ERB)通过抑制 G2/M 细胞周期阶段的微管动力学表现出独特的作用机制。我们利用 T98G 人胶质瘤细胞系来研究 ERB 和 TMZ 单独或联合使用的效果。实验分组如下:对照组、E5 组(5 nM ERB)、T0.75 组(0.75 mM TMZ)、T1 组(1.0 mM TMZ)和组合组(E5+T0.75 和 E5+T1)。所有组的细胞增殖均明显减少。凋亡标记物显示,在 48 小时的时间点上,所有治疗组的附件素-V 表达都出现了时间依赖性增加。联合处理组的 Caspase-3 在 48 小时时点出现增加。透射电子显微镜(TEM)显示,对照组胶质瘤细胞的超微结构特征正常。然而,在球形胶质母细胞瘤模型中,特别是在联合治疗组中,治疗诱导了超微结构的变化。这些变化包括自噬空泡和细胞凋亡形态的剂量依赖性增加。总之,ERB 和 TMZ 作用机制的相似性表明,两者联合使用可能会产生协同效应。我们的研究结果表明,这种联合用药在 48 小时后会诱导胶质瘤球形细胞发生严重损伤和自噬。
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引用次数: 0
The potential protective effect of propolis on diabetic nephropathy induced by streptozotocin in adult albino rats. 蜂胶对链脲佐菌素诱导的成年白化大鼠糖尿病肾病的潜在保护作用。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-08-01 DOI: 10.1080/01913123.2024.2386009
Shaimaa M Hafez, Heba F Ibrahim, Shaimaa R Abdelmohsen, Noha A E Yasin, Yara S Abouelela, Heba Abdelnaser Aboelsoud

Diabetes mellitus is a common metabolic disorder. It is associated with serious life-threatening complications if not properly managed. The current study aimed at investigating the possible protective role of propolis on streptozotocin-induced diabetic nephropathy. A diabetic rat model was induced by a single intraperitoneal injection of 55 mg/kg streptozotocin. After 4 days, the diabetic rats received oral propolis (300 mg/kg/day) via gastric gavage for 28 days. Biochemical, histopathological and ultrastructural evaluations were performed. The results showed that: streptozotocin-induced diabetes was associated with a marked decrease in the serum high-density lipoproteins and antioxidant enzymes. However, a significant elevation in the levels of serum creatinine, blood urea nitrogen, uric acid, cholesterol, triglycerides and low-density lipoproteins was detected. Furthermore, streptozotocin treatment induced histopathological alterations of the renal cortex; in the form of distorted glomerular capillaries, widened Bowman's space and signs of epithelial tubular degeneration. Ultra-structurally, thickening and irregularity of the glomerular basement membrane and podocytes foot processes effacement were observed. The tubular epithelial cells showed swollen vacuolated mitochondria, scarce basal infoldings and loss of microvilli. Conversely, propolis partially restored the normal lipid profile, antioxidant biomarkers and renal cortical morphology. Propolis exhibited a sort of renoprotection through hypoglycemic, anti-hyperlipidemic and antioxidant effects.

糖尿病是一种常见的代谢性疾病。如果处理不当,会出现危及生命的严重并发症。本研究旨在探讨蜂胶对链脲佐菌素诱导的糖尿病肾病可能起到的保护作用。研究人员向糖尿病大鼠腹腔注射55毫克/千克链脲佐菌素,诱导大鼠发生糖尿病肾病。4 天后,糖尿病大鼠通过灌胃口服蜂胶(300 毫克/千克/天),持续 28 天。对大鼠进行了生化、组织病理学和超微结构评估。结果表明:链脲菌素诱导的糖尿病与血清高密度脂蛋白和抗氧化酶的明显降低有关。然而,检测到血清肌酐、血尿素氮、尿酸、胆固醇、甘油三酯和低密度脂蛋白水平明显升高。此外,链脲佐菌素治疗诱发肾皮质组织病理学改变,表现为肾小球毛细血管扭曲、鲍曼间隙增宽和上皮肾小管变性。在超微结构上,观察到肾小球基底膜增厚且不规则,荚膜细胞足突脱落。肾小管上皮细胞显示出肿胀的空泡化线粒体、稀少的基底折叠和微绒毛脱落。相反,蜂胶能部分恢复正常的血脂、抗氧化生物标志物和肾皮质形态。蜂胶通过降血糖、抗高血脂和抗氧化作用表现出某种肾保护作用。
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引用次数: 0
Ameliorative effects of gallic acid on tebuconazole-induced adverse effects in the cerebellum of adult albino rats: histopathological and immunohistochemical evidence. 没食子酸对戊唑醇诱导的成年白化大鼠小脑不良反应的改善作用:组织病理学和免疫组化证据。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1080/01913123.2024.2387685
Omnia I Ismail, Hala Mohamed Hassanin

Tebuconazole (TEB) is a common triazole sterol demethylation inhibitor fungicide utilized to manage a variety of diseases in crops like cereals, fruits, and vegetables. The aim of this work was to assess the effects of TEB on the structure of the cerebellum in adult albino rats and possible protective impact of co-administration of Gallic acid (GA). Four groups of forty adult male albino rats were randomly selected, and the rats in group I received corn oil through daily gavage for 4 weeks. Group II received GA dissolved in the normal saline at a dose of 100 mg/kg through daily gavage for 4 weeks, group III administered with TEB dissolved in corn oil at its acceptable daily intake dose (0.02 mg/kg body weight) through daily gavage for 4 weeks, group IV rats received both TEB and GA. For light microscopic, ultrastructural, and immunohistochemical investigations, cerebellar specimens were prepared. TEB exposure led to neuronal damage in the form of degenerated Purkinje cells with vacuolated cytoplasm, areas of lost Purkinje cells, the basket cells appeared vacuolated with degenerated neuropil, the granule cells clumped with congested areas between them, dilated cerebellar islands, weak positive bcl2 immunoreactions in the Purkinje cells, and numerous GFAP-positive astrocytes. GA mitigated TEB-mediated histological changes in the cerebellar cortex. We concluded that TEB caused Purkinje neurons in the rat cerebellar cortex to degenerate and undergo apoptosis. GA had a neuroprotective benefit against TEB toxicity in the rat cerebellar cortex.

戊唑醇(TEB)是一种常见的三唑类甾醇脱甲基化抑制剂杀菌剂,用于防治谷物、水果和蔬菜等作物的多种病害。这项研究的目的是评估 TEB 对成年白化大鼠小脑结构的影响,以及同时服用没食子酸(GA)可能产生的保护作用。随机选取四组四十只成年雄性白化大鼠,第一组每天灌胃玉米油,持续四周。第二组大鼠每天灌胃 100 毫克/千克剂量溶于生理盐水的 GA,连续 4 周;第三组大鼠每天灌胃 0.02 毫克/千克体重剂量溶于玉米油的 TEB,连续 4 周;第四组大鼠同时灌胃 TEB 和 GA。为了进行光学显微镜、超微结构和免疫组化研究,制备了小脑标本。暴露于 TEB 会导致神经元损伤,表现为具有空泡化胞质的浦肯野细胞变性、浦肯野细胞消失区域、篮状细胞出现空泡化并伴有神经髓变性、颗粒细胞聚集并在它们之间出现充血区域、小脑岛扩张、浦肯野细胞中出现弱阳性 bcl2 免疫反应以及大量 GFAP 阳性星形胶质细胞。GA减轻了TEB介导的小脑皮质组织学变化。我们的结论是,TEB 会导致大鼠小脑皮质中的浦肯野神经元退化和凋亡。GA对大鼠小脑皮质的TEB毒性具有神经保护作用。
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引用次数: 0
Excessive lipid production shapes glioma tumor microenvironment. 过多的脂质生成塑造了胶质瘤的肿瘤微环境。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-08-19 DOI: 10.1080/01913123.2024.2392728
Haitham H Maraqah, John Paul Aboubechara, Mones S Abu-Asab, Han Sung Lee, Orwa Aboud

Disrupted lipid metabolism is a characteristic of gliomas. This study utilizes an ultrastructural approach to characterize the prevalence and distribution of lipids within gliomas. This study made use of tissue from IDH1 wild type (IDH1-wt) glioblastoma (n = 18) and IDH1 mutant (IDH1-mt) astrocytoma (n = 12) tumors. We uncover a prevalent and intriguing surplus of lipids. The bulk of the lipids manifested as sizable cytoplasmic inclusions and extracellular deposits in the tumor microenvironment (TME); in some tumors the lipids were stored in the classical membraneless spheroidal lipid droplets (LDs). Frequently, lipids accumulated inside mitochondria, suggesting possible dysfunction of the beta-oxidation pathway. Additionally, the tumor vasculature have lipid deposits in their lumen and vessel walls; this lipid could have shifted in from the tumor microenvironment or have been produced by the vessel-invading tumor cells. Lipid excess in gliomas stems from disrupted beta-oxidation and dysfunctional oxidative phosphorylation pathways. The implications of this lipid-driven environment include structural support for the tumor cells and protection against immune responses, non-lipophilic drugs, and free radicals.

脂质代谢紊乱是胶质瘤的一个特征。本研究利用超微结构方法来描述胶质瘤中脂质的流行和分布情况。本研究使用了来自IDH1野生型(IDH1-wt)胶质母细胞瘤(n = 18)和IDH1突变型(IDH1-mt)星形细胞瘤(n = 12)肿瘤的组织。我们发现了一种普遍存在且耐人寻味的脂质过剩现象。大部分脂质表现为肿瘤微环境(TME)中的大量细胞质包涵体和胞外沉积物;在一些肿瘤中,脂质储存在经典的无膜球形脂滴(LDs)中。脂质经常积聚在线粒体内,这表明β-氧化途径可能出现了功能障碍。此外,肿瘤血管腔内和血管壁上也有脂质沉积;这些脂质可能是从肿瘤微环境中转移进来的,也可能是血管中的肿瘤细胞产生的。胶质瘤中的脂质过剩源于β-氧化紊乱和氧化磷酸化途径功能失调。这种脂质驱动环境的影响包括为肿瘤细胞提供结构支持,保护其免受免疫反应、非亲脂药物和自由基的伤害。
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引用次数: 0
Features that characterize monoclonal light chain ("myeloma") cast nephropathy with immunofluorescence challenges and emphasis on electron microscopy. 单克隆轻链("骨髓瘤")铸型肾病的特征与免疫荧光挑战,重点是电子显微镜。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-08-21 DOI: 10.1080/01913123.2024.2390892
Guillermo A Herrera, Luan D Truong, Sadhna Dhingra, Elba A Turbat-Herrera

Renal disease is a common cause of morbidity and mortality in patients with plasma cell dyscrasias. The serum-free light chain assay is used in patients, mostly older, with unexplained acute kidney injury to screen for potential myeloma cast nephropathy. This study consists of a systematic review of diagnostic features in myeloma cast nephropathy. The morphological features of tubular casts in patients with multiple myeloma have not been systematically analyzed. This study focuses on the morphology of these casts, emphasizing ultrastructural features, in a series of 23 patients with light chain ("myeloma") cast nephropathy and compared them with casts in 10 patients with various diseases. The immunofluorescence data were correlated with morphological findings to provide diagnostic assessments and practice guidelines. The ultrastructural features identified as diagnostic of casts associated with myeloma included: amyloid and crystals in the casts, multiple well-defined fracture planes forming a complex jigsaw puzzle arrangement of cast contents, indicative of the fragility of the immunoglobulin light chains involved, and reactive tubular cells lining the tubules with the casts. These features were seen in 95.2% of MCN cases and none of the casts in other renal conditions. Myeloma casts exhibited light chain monoclonality in a significant percentage of the MCN cases and often no staining for IgA or IgM. In contrast, the majority of non-myeloma casts stained for both kappa and lambda light chains, lgA, and lgM, and showed ultrastructurally a rather uniform finely to coarsely granular electron density occasionally admixed with cellular debris.

肾脏疾病是浆细胞异常患者发病和死亡的常见原因。无血清轻链测定可用于不明原因的急性肾损伤患者(多为老年人),以筛查潜在的骨髓瘤铸型肾病。本研究对骨髓瘤铸型肾病的诊断特征进行了系统回顾。目前尚未对多发性骨髓瘤患者肾小管铸型的形态特征进行系统分析。本研究重点研究了一系列 23 例轻链("骨髓瘤")铸型肾病患者的这些铸型的形态,强调了超微结构特征,并将其与 10 例不同疾病患者的铸型进行了比较。免疫荧光数据与形态学结果相互关联,从而提供诊断评估和实践指南。经鉴定,可诊断骨髓瘤相关铸型肾病的超微结构特征包括:铸型中的淀粉样蛋白和结晶、多个界限清晰的断裂面形成铸型内容物的复杂拼图排列(表明相关免疫球蛋白轻链的脆性),以及反应性小管细胞与铸型肾病患者的肾小管相衬。在 95.2% 的 MCN 病例中出现了这些特征,而在其他肾脏疾病中却没有出现这些铸型。在相当大比例的 MCN 病例中,骨髓瘤铸型表现出轻链单克隆性,而且通常没有 IgA 或 IgM 染色。与此相反,大多数非骨髓瘤铸型都对 kappa 和 lambda 轻链、lgA 和 lgM 进行了染色,并在超微结构上显示出相当均匀的细粒至粗粒电子密度,偶尔会混有细胞碎片。
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引用次数: 0
Emperipolesis in pleural fluid mesothelial cells. A phenomenon not associated with Rosai-Dorfman disease, report of a case. 胸腔积液间皮细胞中的包膜。一个与罗赛-多夫曼病无关的现象,一个病例的报告。
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-09-02 Epub Date: 2024-07-30 DOI: 10.1080/01913123.2024.2382987
Diana Sofía Vargas-Lías, Mónica Lizette Serrano-Arévalo, Lara-Torres César, Corredor-Alonso Guillermo Ernesto, Hugo R Dominguez-Malagon

Emperipolesis is a cell-within-cell phenomenon distinct from phagocytosis more often described in Rosai-Dorfman disease, where usually lymphocytes or other bone marrow cells (plasma cells, erythroblasts or neutrophils) are entirely surrounded but not engulfed by macrophages as the host cell, but occasionally megakaryocytes and neoplastic could be. Mesothelial cell has been described in a couple of cases of lymphomas affecting serous membranes, but never described in pleuritis. In the present work, the first case of emperipolesis by mesothelial cells in a patient with self-limited pleural effusion was demonstrated by immunohistochemistry and Electron Microscopy studies.

通常淋巴细胞或其他骨髓细胞(浆细胞、红细胞或中性粒细胞)会完全被巨噬细胞包围,但不会像宿主细胞那样被巨噬细胞吞噬,偶尔巨核细胞和肿瘤细胞也会被吞噬。在几例影响浆膜的淋巴瘤病例中曾描述过间皮细胞,但从未在胸膜炎中描述过。在本研究中,通过免疫组化和电子显微镜研究,首次在一名自限性胸腔积液患者中证实了间皮细胞引起的胸膜炎。
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引用次数: 0
Can the analysis of chromatin texture and nuclear fractal dimensions serve as effective means to distinguish non-invasive follicular thyroid neoplasm with papillary-like nuclear features from other malignancies with follicular pattern in the thyroid?: a study. 染色质纹理和核分形尺寸分析能否作为有效手段,将具有乳头状核特征的非侵袭性甲状腺滤泡性肿瘤与其他具有甲状腺滤泡形态的恶性肿瘤区分开来?
IF 1.1 4区 医学 Q4 MICROSCOPY Pub Date : 2024-07-03 Epub Date: 2024-06-03 DOI: 10.1080/01913123.2024.2362758
Geet Bhuyan, Anjumoni Rabha

Objective: Thyroid carcinoma ranks as the 9th most prevalent global cancer, accounting for 586,202 cases and 43,636 deaths in 2020. Computerized image analysis, utilizing artificial intelligence algorithms, emerges as a potential tool for tumor evaluation.

Aim: This study aims to assess and compare chromatin textural characteristics and nuclear dimensions in follicular neoplasms through gray-level co-occurrence matrix (GLCM), fractal, and morphometric analysis.

Method: A retrospective cross-sectional study involving 115 thyroid malignancies, specifically 49 papillary thyroid carcinomas with follicular morphology, was conducted from July 2021 to July 2023. Ethical approval was obtained, and histopathological examination, along with image analysis, was performed using ImageJ software.

Results: A statistically significant difference was observed in contrast (2.426 (1.774-3.412) vs 2.664 (1.963-3.610), p = .002), correlation (1.202 (1.071-1.298) vs 0.892 (0.833-0.946), p = .01), and ASM (0.071 (0.090-0.131) vs 0.044 (0.019-0.102), p = .036) between NIFTP and IFVPTC. However, morphometric parameters did not yield statistically significant differences among histological variants.

Conclusion: Computerized image analysis, though promising in subtype discrimination, requires further refinement and integration with traditional diagnostic parameters. The study suggests potential applications in scenarios where conventional histopathological assessment faces limitations due to limited tissue availability. Despite limitations such as a small sample size and a retrospective design, the findings contribute to understanding thyroid carcinoma characteristics and underscore the need for comprehensive evaluations integrating various diagnostic modalities.

目的:甲状腺癌在全球癌症发病率中排名第九,2020 年将有 586 202 例发病,43 636 例死亡。目的:本研究旨在通过灰度级共现矩阵(GLCM)、分形和形态计量分析,评估和比较滤泡性肿瘤的染色质纹理特征和核尺寸:方法:2021 年 7 月至 2023 年 7 月进行了一项回顾性横断面研究,涉及 115 例甲状腺恶性肿瘤,特别是 49 例具有滤泡形态的甲状腺乳头状癌。研究获得了伦理批准,并使用 ImageJ 软件进行了组织病理学检查和图像分析:结果:NIFTP 和 IFVPTC 的对比度(2.426 (1.774-3.412) vs 2.664 (1.963-3.610),p = .002)、相关度(1.202 (1.071-1.298) vs 0.892 (0.833-0.946),p = .01)和 ASM(0.071 (0.090-0.131) vs 0.044 (0.019-0.102),p = .036)差异有统计学意义。然而,形态计量参数在组织学变异之间并没有产生统计学意义上的显著差异:结论:计算机图像分析虽然在亚型鉴别方面很有前景,但还需要进一步完善并与传统诊断参数相结合。这项研究表明,在传统组织病理学评估因组织可用性有限而面临限制的情况下,计算机图像分析具有潜在的应用价值。尽管存在样本量小和回顾性设计等局限性,但研究结果有助于了解甲状腺癌的特征,并强调了综合各种诊断方式进行全面评估的必要性。
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Ultrastructural Pathology
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