{"title":"Knowledge sharing is the key for the progress of science","authors":"S. Vari","doi":"10.15407/ubj90.02.005","DOIUrl":"https://doi.org/10.15407/ubj90.02.005","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"5-6"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42093316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was investigated of levels of oxidative modification of lipoproteins and content of plasminogen/plasmin system components – tissue-type plasminogen activator (t-pa) and plasminogen activators inhibitor-1 (paI-1), in patients with stage II arterial hypertension (ahT) and resistant form of ahT. It was established that t-pa level in blood plasma of the patients is 2 times lower under stage II hypertension than normal and 2.5 times lower under resistant ahT. The inhibitor activity is 1.5 and 2 times higher consequently. It is concluded that patients with AHT have a decreased fibrinolytic potential, which can cause thrombotic states. Our evaluation showed a significant accumulation of products of lipid and protein oxidation, decrease of activity of antioxidant enzymes and changes of the activity of high density-lipoproteins-associated enzymes (decrease of paraoxonase-1 activity, increase of myeloperoxidase activity). Oxidized lipoproteins, t-pa and PAI-1 can be used as prognostic markers of development of complications and for evaluating the efficacy of therapy in patients with arterial hypertension.
{"title":"Determination of plasminogen/plasmin system components and indicators of lipoproteins oxidative modification under arterial hypertension","authors":"O. Yusova, O. Savchuk, T. Grinenko, O. Kuchmenko","doi":"10.15407/UBJ90.01.058","DOIUrl":"https://doi.org/10.15407/UBJ90.01.058","url":null,"abstract":"The present study was investigated of levels of oxidative modification of lipoproteins and content of plasminogen/plasmin system components – tissue-type plasminogen activator (t-pa) and plasminogen activators inhibitor-1 (paI-1), in patients with stage II arterial hypertension (ahT) and resistant form of ahT. It was established that t-pa level in blood plasma of the patients is 2 times lower under stage II hypertension than normal and 2.5 times lower under resistant ahT. The inhibitor activity is 1.5 and 2 times higher consequently. It is concluded that patients with AHT have a decreased fibrinolytic potential, which can cause thrombotic states. Our evaluation showed a significant accumulation of products of lipid and protein oxidation, decrease of activity of antioxidant enzymes and changes of the activity of high density-lipoproteins-associated enzymes (decrease of paraoxonase-1 activity, increase of myeloperoxidase activity). Oxidized lipoproteins, t-pa and PAI-1 can be used as prognostic markers of development of complications and for evaluating the efficacy of therapy in patients with arterial hypertension.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"58-67"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49078438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. V. Danylovych, T. V. Bohach, Yuriy V. Danylovych
Modern data on biochemical patterns of nitric oxide biosynthesis in mammal cells from l-arginine in normoxic conditions is described. The attention of the authors is given to the results of the recent years on the structure and regulation features isoforms of No-synthase. The emphasis is put on the latest conception of the compartmentalization of certain isoforms of these enzymes in cells and on the possibility of the directed transport of nitric oxide in the vascular wall. The central place in the review is devoted to issues on the endogenous formation of NO in mitochondria and its potential physiological significance. Our own results on the identification of NO in mitochondria of the uterine smooth muscle, biochemical characteristics of this process and NO possible role in Са2+ transport regulation by organelles are presented and discussed.
{"title":"The biosynthesis of nitric oxide from L-arginine. Nitric oxide formation features and its functional role in mitochondria","authors":"G. V. Danylovych, T. V. Bohach, Yuriy V. Danylovych","doi":"10.15407/UBJ90.01.003","DOIUrl":"https://doi.org/10.15407/UBJ90.01.003","url":null,"abstract":"Modern data on biochemical patterns of nitric oxide biosynthesis in mammal cells from l-arginine in normoxic conditions is described. The attention of the authors is given to the results of the recent years on the structure and regulation features isoforms of No-synthase. The emphasis is put on the latest conception of the compartmentalization of certain isoforms of these enzymes in cells and on the possibility of the directed transport of nitric oxide in the vascular wall. The central place in the review is devoted to issues on the endogenous formation of NO in mitochondria and its potential physiological significance. Our own results on the identification of NO in mitochondria of the uterine smooth muscle, biochemical characteristics of this process and NO possible role in Са2+ transport regulation by organelles are presented and discussed.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"3-24"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49283471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Innovative activity of Palladin Institute of Biochemistry of NAS of Ukraine: problems and prospects","authors":"G. G. L. S. V. Komisarenko","doi":"10.15407/ubj90.01.077","DOIUrl":"https://doi.org/10.15407/ubj90.01.077","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"77-99"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44206658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Affibody molecules are small protein scaffolds mostly based on triple-helical bundle protein domains. Many triple helix-based affibody proteins have shown prominent properties for tumor imaging and therapy. In our opinion, the structural organizations and the sizes of affibody molecules could be modified to increase their recognition abilities and binding affinities to human epidermal growth factor receptor type 2 (HER2). Thereby, the purpose of this study was to design and characterize a novel platform of affibody molecule consisting of five separate helixes (encoding 99 amino acids with a duplicate of helixes 1 and 2 at N-terminus plus GGGC chelator peptide sequence at C-terminus) enable of binding to HER2 with higher avidity. Using in silico screening methods, the structure and the interactive potential of designed affibody was comparatively investigated. The molecular expression and production of the designed affibody in Escherichia coli cells was successfully examined and reported.
{"title":"Designing, docking and heterologous expression of an anti-HER2 affibody molecule","authors":"N. Tabasi, A. Gholizadeh, B. Kohnehrouz","doi":"10.15407/UBJ90.01.068","DOIUrl":"https://doi.org/10.15407/UBJ90.01.068","url":null,"abstract":"Affibody molecules are small protein scaffolds mostly based on triple-helical bundle protein domains. Many triple helix-based affibody proteins have shown prominent properties for tumor imaging and therapy. In our opinion, the structural organizations and the sizes of affibody molecules could be modified to increase their recognition abilities and binding affinities to human epidermal growth factor receptor type 2 (HER2). Thereby, the purpose of this study was to design and characterize a novel platform of affibody molecule consisting of five separate helixes (encoding 99 amino acids with a duplicate of helixes 1 and 2 at N-terminus plus GGGC chelator peptide sequence at C-terminus) enable of binding to HER2 with higher avidity. Using in silico screening methods, the structure and the interactive potential of designed affibody was comparatively investigated. The molecular expression and production of the designed affibody in Escherichia coli cells was successfully examined and reported.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"68-76"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48765186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The research deals with the determination of the activity of aldehyde dehydrogenase (Ec 1.2.1.3), aldehyde reductase (Ec 1.1.1.21) as well as the content of TBa reactive substances and protein carbonyl derivates in the rat liver cytosolic fraction under the conditions of acetaminophen-induced hepatitis and protein deficiency. The most pronounced decrease in the activity of enzymes utilizing endogenous aldehydes is observed in the liver cytosolic fraction of animals with toxic liver injury maintained under the conditions of alimentary protein deficiency. Meanwhile, the accumulation of TBA reactive substances and protein carbonyl-derivates in the liver cytosolic fraction of animals of this experimental group was established. The accumulation of aldehyde products of lipid and protein oxidative damage on the background of the reduction in the activity of enzymes providing aldehyde catabolism may be considered as a possible mechanism underlying hepatocyte dysfunction under the conditions of toxic damage in protein-deficient animals.
{"title":"Activity of the mitochondrial isoenzymes of endogenous aldehydes catabolism under the conditions of acetaminophen-induced hepatitis","authors":"O. Voloshchuk, G. Kopylchuk, Y. Mishyna","doi":"10.15407/UBJ90.01.042","DOIUrl":"https://doi.org/10.15407/UBJ90.01.042","url":null,"abstract":"The research deals with the determination of the activity of aldehyde dehydrogenase (Ec 1.2.1.3), aldehyde reductase (Ec 1.1.1.21) as well as the content of TBa reactive substances and protein carbonyl derivates in the rat liver cytosolic fraction under the conditions of acetaminophen-induced hepatitis and protein deficiency. The most pronounced decrease in the activity of enzymes utilizing endogenous aldehydes is observed in the liver cytosolic fraction of animals with toxic liver injury maintained under the conditions of alimentary protein deficiency. Meanwhile, the accumulation of TBA reactive substances and protein carbonyl-derivates in the liver cytosolic fraction of animals of this experimental group was established. The accumulation of aldehyde products of lipid and protein oxidative damage on the background of the reduction in the activity of enzymes providing aldehyde catabolism may be considered as a possible mechanism underlying hepatocyte dysfunction under the conditions of toxic damage in protein-deficient animals.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"42-47"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49135824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Fafula, G. V. Danylovych, A. Besedina, O. Melnyk, Z. Vorobets
the present study was undertaken to evaluate [ca]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. the [ca]i reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. there were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [ca]i. was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. the 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [ca]i in normoand pathozoospermic samples. the mechanisms of progesterone-dependent activation of the ca2+-entry and the functioning of the voltage gated ca2+-channels of plasmalemma are disturbed in pathologies – there was no increase in the ca2+ level in the conditions of k+-depolarization (in the presence of inhibitors of k+-channels).
{"title":"Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia","authors":"R. Fafula, G. V. Danylovych, A. Besedina, O. Melnyk, Z. Vorobets","doi":"10.15407/UBJ90.01.048","DOIUrl":"https://doi.org/10.15407/UBJ90.01.048","url":null,"abstract":"the present study was undertaken to evaluate [ca]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. the [ca]i reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. there were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [ca]i. was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. the 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [ca]i in normoand pathozoospermic samples. the mechanisms of progesterone-dependent activation of the ca2+-entry and the functioning of the voltage gated ca2+-channels of plasmalemma are disturbed in pathologies – there was no increase in the ca2+ level in the conditions of k+-depolarization (in the presence of inhibitors of k+-channels).","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"48-57"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48247112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. V. Danylovych, О. V. Kоlomiets, Yuriy V. Danylovych, Rodik Rv, V. I. Kаlchenko, S. О. Kоsterin
it was shown that calix[4]arene c-956 exhibited a pronounced concentration-dependent (10-100 μm) inhibitory effect on the h+-ca2+-exchanger of the inner mitochondrial membrane of rat uterine myocytes (Ki 35.1 ± 7.9 μm). the inhibitory effect of calix[4]arene c-956 was accompanied by a decrease in the initial rate (V0 ) and an increase in the magnitude of the characteristic time (τ1/2) of the ΔрН-induced Са 2+ release. At the same time, it did not affect the potential-dependent accumulation of ca2+ in mitochondria. thus, the action of calix[4]arene c-956 might be directed on increasing the concentration of ca ions in the mitochondrial matrix. the calculation of basic kinetic parameters of the ca2+ transport from isolated organelles (in the case of its non-zero stationary level), based on changes in fluorescence of ca2+-sensitive dye Fluo-4 Am in mitochondria was performed. the proposed approach can be used for the kinetic analysis of the exponential decrease of the fluorescence response of any probes under the same experimental conditions.
{"title":"Cаlіx[4]аrene С-956 is effective inhibitor of Н(+)-Сa(2+)-exchanger in smooth muscle mitochondria","authors":"G. V. Danylovych, О. V. Kоlomiets, Yuriy V. Danylovych, Rodik Rv, V. I. Kаlchenko, S. О. Kоsterin","doi":"10.15407/UBJ90.01.025","DOIUrl":"https://doi.org/10.15407/UBJ90.01.025","url":null,"abstract":"it was shown that calix[4]arene c-956 exhibited a pronounced concentration-dependent (10-100 μm) inhibitory effect on the h+-ca2+-exchanger of the inner mitochondrial membrane of rat uterine myocytes (Ki 35.1 ± 7.9 μm). the inhibitory effect of calix[4]arene c-956 was accompanied by a decrease in the initial rate (V0 ) and an increase in the magnitude of the characteristic time (τ1/2) of the ΔрН-induced Са 2+ release. At the same time, it did not affect the potential-dependent accumulation of ca2+ in mitochondria. thus, the action of calix[4]arene c-956 might be directed on increasing the concentration of ca ions in the mitochondrial matrix. the calculation of basic kinetic parameters of the ca2+ transport from isolated organelles (in the case of its non-zero stationary level), based on changes in fluorescence of ca2+-sensitive dye Fluo-4 Am in mitochondria was performed. the proposed approach can be used for the kinetic analysis of the exponential decrease of the fluorescence response of any probes under the same experimental conditions.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"25-33"},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47855804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beydolah Shahouzehi, M. Shabani, N. Shahrokhi, Sakine Sadeghiyan, Y. Masoumi-Ardakani
{"title":"Effects of Pistacia atlantica resin oil on the level of VEGF, hydroxyproline, antioxidant and wound healing activity in STZ-induced diabetic rats","authors":"Beydolah Shahouzehi, M. Shabani, N. Shahrokhi, Sakine Sadeghiyan, Y. Masoumi-Ardakani","doi":"10.15407/ubj90.01.034","DOIUrl":"https://doi.org/10.15407/ubj90.01.034","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"34-41"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67021975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Shahouzehi, K. Barkhordari, S. Aminizadeh, Y. Masoumi-Ardakanі
1Cardiovascular research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; e-mail: bshahouzehi@yahoo.com; 2department of Virology, afzalipour School of medicine, Kerman University of medical Sciences, Kerman, Iran; e-mail: khabatzanbil@gmail.com; 3Physiology research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; e-mail: soheilaminizadeh@gmail.com; 4Physiology research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; *e-mail: ymab125@yahoo.com
{"title":"Effect of L-carnitine administration on serum insulin and adiponectin levels, and AMPK, APPL1 and PPAR? gene expression in STZ-induced diabetic rat liver","authors":"B. Shahouzehi, K. Barkhordari, S. Aminizadeh, Y. Masoumi-Ardakanі","doi":"10.15407/UBJ89.06.048","DOIUrl":"https://doi.org/10.15407/UBJ89.06.048","url":null,"abstract":"1Cardiovascular research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; e-mail: bshahouzehi@yahoo.com; 2department of Virology, afzalipour School of medicine, Kerman University of medical Sciences, Kerman, Iran; e-mail: khabatzanbil@gmail.com; 3Physiology research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; e-mail: soheilaminizadeh@gmail.com; 4Physiology research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of medical Sciences, Kerman, Iran; *e-mail: ymab125@yahoo.com","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"89 1","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"2017-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46818420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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