Т. V. Gudzenko, O. Voliuvach, O. Gorshkova, А. M. Ostapchuk, V. Ivanytsia
the aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis onu551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis onu551 strain was performed using an automatic system for microorganisms’ identification Midi sherlock (Midi, usa) based on gas chromatograph agilent 7890. chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis onu551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. the total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). the saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). no 2and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis onu551 strain. unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), ∑17:1 iso i/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis onu551 strain. according to the fatty acid profile analysis with Midi sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563).
本研究的目的是测定枯草芽孢杆菌onu551菌株的细胞脂质脂肪酸组成，以鉴定该菌株是一种苯酚破坏者。采用基于agilent 7890气相色谱仪的微生物自动鉴定系统Midi sherlock (Midi, usa)对枯草芽孢杆菌onu551进行了脂肪酸分析。色谱分析表明，枯草芽孢杆菌onu551的脂肪酸谱主要由饱和酸的支链异构体组成:13-甲基十四烷酸(15:0 iso;34.72%)和12-甲基十四烷(15:0);33.72%)酸。支链饱和脂肪酸总含量为88.16% ~ 14:0 iso(0.52%)、15:0 iso(34.72%)、15:0 anteiso(33.72%)、16:0 iso(1.85%)、17:0 iso(7.11%)、17:0 anteiso(10.24%)。正常结构的饱和脂肪酸含量分别为- 12:0(0.36%)、14:0(0.28%)、16:0(1.30%)。枯草芽孢杆菌onu551的脂肪酸谱未检出2、3-羟基酸，未检出环状脂肪酸。不饱和脂肪酸异构体- 15:1 w5c(1.85%)、16:1 w11c(1.21%)、16:1 w7c醇(1.08%)、17:1 iso w10c(3.18%)、∑17:1 iso i/anteiso B(2.57%)是枯草芽孢杆菌onu551的独特生物标志物。根据Midi sherlock系统的脂肪酸谱分析，鉴定菌株为枯草芽孢杆菌，相似度较高(0.563)。
{"title":"Fatty acids composition of Bacillus subtilis ONU551 lipids","authors":"Т. V. Gudzenko, O. Voliuvach, O. Gorshkova, А. M. Ostapchuk, V. Ivanytsia","doi":"10.15407/ubj91.06.096","DOIUrl":"https://doi.org/10.15407/ubj91.06.096","url":null,"abstract":"the aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis onu551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis onu551 strain was performed using an automatic system for microorganisms’ identification Midi sherlock (Midi, usa) based on gas chromatograph agilent 7890. chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis onu551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. the total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). the saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). no 2and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis onu551 strain. unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), ∑17:1 iso i/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis onu551 strain. according to the fatty acid profile analysis with Midi sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563).","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45431409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Korotkyi, K. Dvorshchenko, A. Vovk, A. Dranitsina, M. Tymoshenko, L. Kot, L. Ostapchenko
Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant).
{"title":"Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti","authors":"O. Korotkyi, K. Dvorshchenko, A. Vovk, A. Dranitsina, M. Tymoshenko, L. Kot, L. Ostapchenko","doi":"10.15407/ubj91.06.049","DOIUrl":"https://doi.org/10.15407/ubj91.06.049","url":null,"abstract":"Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant).","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"49-58"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44108813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1Physiology research Center, Institute of Basic and Clinical Physiology Sciences, kerman University of Medical Sciences, kerman, Iran; 2Student research Committee, School of Medicine, kerman University of Medical Sciences, kerman, Iran; 3Cardiovascular research Center, Institute of Basic and Clinical Physiology Sciences, kerman University of Medical Sciences, kerman, Iran; e-mail: bshahouzehi@yahoo.com, bshahouzehi@gmail.com
{"title":"Carnitine effects on serum and pancreas inflammatory response in diabetic rats","authors":"Y. Masoumi-Ardakani, H. Fallah, B. Shahouzehi","doi":"10.15407/ubj91.06.059","DOIUrl":"https://doi.org/10.15407/ubj91.06.059","url":null,"abstract":"1Physiology research Center, Institute of Basic and Clinical Physiology Sciences, kerman University of Medical Sciences, kerman, Iran; 2Student research Committee, School of Medicine, kerman University of Medical Sciences, kerman, Iran; 3Cardiovascular research Center, Institute of Basic and Clinical Physiology Sciences, kerman University of Medical Sciences, kerman, Iran; e-mail: bshahouzehi@yahoo.com, bshahouzehi@gmail.com","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"59-66"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44907648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Mankovska, G. Gerashchenko, E. Rozenberg, E. Stakhovsky, O. Kononenko, Y. Bondarenko, V. Kashuba
{"title":"Analysis of Aurora kinases genes expression points on their distinct roles in prostate cancer development","authors":"O. Mankovska, G. Gerashchenko, E. Rozenberg, E. Stakhovsky, O. Kononenko, Y. Bondarenko, V. Kashuba","doi":"10.15407/ubj91.06.015","DOIUrl":"https://doi.org/10.15407/ubj91.06.015","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"15-26"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42945873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. V. Pyrogova, G. K. Bereznitsky, G. K. Gogolinskaya, T. Platonova, I. M. Kolesnikova, O. O. MaSeNKo, R. Marunich, Yuriy Ushenin, Y. Makogonenko, E. Lugovskoi
The effect of NaCl and NaF salts in the range of 0.1-0.225 M concentrations on individual stages of fibrin polymerization was investigated, namely: the rate of fibrinogen activation by thrombin, the rate of protofibrils formation, the rate of lateral association of protofibrils, and the maximum clot absorbance value at 350 nm. It was found that the chlorine and fluorine anions equally inhibit the rate of formation of fibrin from fibrinogen and the formation of protofibrils activated with thrombin. Chlorine anions were shown to be significantly more effective than fluorine anions, inhibiting the rate of lateral association and maximal clot absorban ce level from fibrin desA and desAB. A component of the inhibitory action of chlorine anions, not related to the ionic strength of the solution, was identified and its effect on the individual polymerization steps was shown. Chlorine anions were found to bind to a fibrin clot. Using the surface plasmon resonance method and fibrin-specific mAb FnI-3c, it was established that the rate of exposure of neoantigenic determinants of mAb in the hinge regions of the fibrinogen molecule during its transformation into fibrin under the action of thrombin is inhibited by chlorine anions in correlation with inhibition of the protofibril lateral association rate. It has been suggested that the inhibitory effect of chlorine anions consists of an ionic component and a component that blocks the conformational mobility of the molecule by the chlorine anions binding to its hinge regions and polymerization sites.
{"title":"Comparative analysis of the influence of chlorine and fluorine anions on the fibrin polymerization","authors":"L. V. Pyrogova, G. K. Bereznitsky, G. K. Gogolinskaya, T. Platonova, I. M. Kolesnikova, O. O. MaSeNKo, R. Marunich, Yuriy Ushenin, Y. Makogonenko, E. Lugovskoi","doi":"10.15407/ubj91.06.027","DOIUrl":"https://doi.org/10.15407/ubj91.06.027","url":null,"abstract":"The effect of NaCl and NaF salts in the range of 0.1-0.225 M concentrations on individual stages of fibrin polymerization was investigated, namely: the rate of fibrinogen activation by thrombin, the rate of protofibrils formation, the rate of lateral association of protofibrils, and the maximum clot absorbance value at 350 nm. It was found that the chlorine and fluorine anions equally inhibit the rate of formation of fibrin from fibrinogen and the formation of protofibrils activated with thrombin. Chlorine anions were shown to be significantly more effective than fluorine anions, inhibiting the rate of lateral association and maximal clot absorban ce level from fibrin desA and desAB. A component of the inhibitory action of chlorine anions, not related to the ionic strength of the solution, was identified and its effect on the individual polymerization steps was shown. Chlorine anions were found to bind to a fibrin clot. Using the surface plasmon resonance method and fibrin-specific mAb FnI-3c, it was established that the rate of exposure of neoantigenic determinants of mAb in the hinge regions of the fibrinogen molecule during its transformation into fibrin under the action of thrombin is inhibited by chlorine anions in correlation with inhibition of the protofibril lateral association rate. It has been suggested that the inhibitory effect of chlorine anions consists of an ionic component and a component that blocks the conformational mobility of the molecule by the chlorine anions binding to its hinge regions and polymerization sites.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"27-37"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48476690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The contribution of the Nobel laureates to the study of lipid metabolism and its regulation. F. Lynen, K. Bloch, S. Bergstrom, B. Samuelsson, J. Vane, M. Brown, J. Goldstein","authors":"O. Matyshevska, V. M. Danilova, S. Komisarenko","doi":"10.15407/ubj91.06.129","DOIUrl":"https://doi.org/10.15407/ubj91.06.129","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"129-143"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48610648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Vovkun, P. Yanchuk, L. Shtanova, S. Veselsky, N. Filimonova, I. Komarov
Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the flavonoid family and the most prominent dietary antioxidants. Numerous pharmacological effects of quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, and cerebrovascular disea ses. Corvitin modulates liver blood flow but its effects on liver lipid metabolism have not been understood. We investigated the influence of corvitin (2.5, 5, 10 mg/kg) on the formation and secretion of cholesterol (Chol), cholesterol esters (EChol), phospholipids (PLs), free fatty acids (FFAs), and triglycerides (TGs) into the bile. Secreted bile was collected during 2.5 h of the experiment. Lipid fractions of the bile were separated by thin-layer chromatography. We defined that administration of corvitin caused a significant (P < 0.05– 0.001) increase in levels of all studied lipid components of bile. At a dose of 2.5 and 5 mg/kg corvitin increased bile content of Chol, PLs and FFAs,and at a dose of 10 mg/kg had the largest effect on the total production of EChol and TGs. We concluded that corvitin activates the liver lipids metabolism and processes of bile formation.
{"title":"Corvitin modulates the content of lipids in rat liver bile","authors":"T. Vovkun, P. Yanchuk, L. Shtanova, S. Veselsky, N. Filimonova, I. Komarov","doi":"10.15407/ubj91.06.112","DOIUrl":"https://doi.org/10.15407/ubj91.06.112","url":null,"abstract":"Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the flavonoid family and the most prominent dietary antioxidants. Numerous pharmacological effects of quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, and cerebrovascular disea ses. Corvitin modulates liver blood flow but its effects on liver lipid metabolism have not been understood. We investigated the influence of corvitin (2.5, 5, 10 mg/kg) on the formation and secretion of cholesterol (Chol), cholesterol esters (EChol), phospholipids (PLs), free fatty acids (FFAs), and triglycerides (TGs) into the bile. Secreted bile was collected during 2.5 h of the experiment. Lipid fractions of the bile were separated by thin-layer chromatography. We defined that administration of corvitin caused a significant (P < 0.05– 0.001) increase in levels of all studied lipid components of bile. At a dose of 2.5 and 5 mg/kg corvitin increased bile content of Chol, PLs and FFAs,and at a dose of 10 mg/kg had the largest effect on the total production of EChol and TGs. We concluded that corvitin activates the liver lipids metabolism and processes of bile formation.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"112-121"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47120278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Grinenko, О. Yusova, O. V. Revka, I. I. Patalakh, T. Yatsenko
Platelets play a key role in hemostasis as cofactors of thrombin generation, fibrin polymerization centers , and initiators of clot retraction, while their ability to modulate clot dissolution remains less understood. The aim of this study was to investigate the interaction of plasminogen and tissue plasminogen activator with native and activated platelets, to determine the amount of plasmin generated by various activators in the presence of platelets, and the ability of platelets to modulate the rate of polymer fibrin hydrolysis. spectrometric and immunofluorometric methods were used in the study. it was shown that intact circulating platelets carry a small amount of plasminogen on their surface, whereas thrombin-induced activation led to the exposure of plasminogen-binding sites on their plasma membrane. activated platelets stimulated plasminogen activation by tissue plasminogen activator, urokinase, and streptokinase. Components of prothrombin complex enhanced plasminogen activation by tissue plasminogen activator on the surface of activated platelets. Model system with desaB-fibrin revealed the ability of platelets to stimulate fibrinolysis. These results suggest that the regulation of fibrinolysis by platelets is provided by the binding of plasminogen and plasminogen activators on their surface, acceleration of plasmin generation and, consequently, acceleration of the onset of fibrin lysis and reducing of the clot lifetime, which is important to maintain hemostatic balance.
{"title":"Fibrinolysis regulation by platelets retaining plasminogen and tissue-type plasminogen activator on their surface","authors":"T. Grinenko, О. Yusova, O. V. Revka, I. I. Patalakh, T. Yatsenko","doi":"10.15407/ubj91.06.038","DOIUrl":"https://doi.org/10.15407/ubj91.06.038","url":null,"abstract":"Platelets play a key role in hemostasis as cofactors of thrombin generation, fibrin polymerization centers , and initiators of clot retraction, while their ability to modulate clot dissolution remains less understood. The aim of this study was to investigate the interaction of plasminogen and tissue plasminogen activator with native and activated platelets, to determine the amount of plasmin generated by various activators in the presence of platelets, and the ability of platelets to modulate the rate of polymer fibrin hydrolysis. spectrometric and immunofluorometric methods were used in the study. it was shown that intact circulating platelets carry a small amount of plasminogen on their surface, whereas thrombin-induced activation led to the exposure of plasminogen-binding sites on their plasma membrane. activated platelets stimulated plasminogen activation by tissue plasminogen activator, urokinase, and streptokinase. Components of prothrombin complex enhanced plasminogen activation by tissue plasminogen activator on the surface of activated platelets. Model system with desaB-fibrin revealed the ability of platelets to stimulate fibrinolysis. These results suggest that the regulation of fibrinolysis by platelets is provided by the binding of plasminogen and plasminogen activators on their surface, acceleration of plasmin generation and, consequently, acceleration of the onset of fibrin lysis and reducing of the clot lifetime, which is important to maintain hemostatic balance.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"38-48"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49633420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cisplatin is commonly used as a chemotherapeutic agent useful in the treatment of several forms of cancer, but its use is limited due to the undesirable side effects of nephrotoxicity. Most of the previous researches found a positive effect of using selenium as an antioxidant on the toxicity of cisplatin during short term administrations although the recommended dose regimen of cisplatin in chemotherapy is multiple successive administration every three or four weeks depending on the type of the tumor. The aim of this study was to examine the effects of long term usage of selenium or nano-selenium on cisplatin-induced nephrotoxicity in albino rats. forty rats were divided into equal four groups, 1st group as a control injected with normal saline, 2nd group injected with cisplatin 6 mg/kg every 21 days for 70 days (experimental period), 3rd group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg selenium in the form of sodium selenite every 3 days during the experimental period, the 4th group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg nano-selenium every 3 days during the experimental period. The results indicated that selenium or nano-selenium exerted an antioxidant effect through increasing the level of antioxidant enzymes in both serum and kidney tissue, while, it shows a negative effect on kidney function through increasing serum urea and creatinine concentrations and causing abnormal morphology of kidney tissue for rats treated with cisplatin during experimental period.
{"title":"Effect of selenium and nano-selenium on cisplatin-induced nephrotoxicity in albino rats","authors":"M. Shafaee, H. Mohamed, S. A. Ahmed, M. Kandeil","doi":"10.15407/ubj91.06.086","DOIUrl":"https://doi.org/10.15407/ubj91.06.086","url":null,"abstract":"Cisplatin is commonly used as a chemotherapeutic agent useful in the treatment of several forms of cancer, but its use is limited due to the undesirable side effects of nephrotoxicity. Most of the previous researches found a positive effect of using selenium as an antioxidant on the toxicity of cisplatin during short term administrations although the recommended dose regimen of cisplatin in chemotherapy is multiple successive administration every three or four weeks depending on the type of the tumor. The aim of this study was to examine the effects of long term usage of selenium or nano-selenium on cisplatin-induced nephrotoxicity in albino rats. forty rats were divided into equal four groups, 1st group as a control injected with normal saline, 2nd group injected with cisplatin 6 mg/kg every 21 days for 70 days (experimental period), 3rd group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg selenium in the form of sodium selenite every 3 days during the experimental period, the 4th group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg nano-selenium every 3 days during the experimental period. The results indicated that selenium or nano-selenium exerted an antioxidant effect through increasing the level of antioxidant enzymes in both serum and kidney tissue, while, it shows a negative effect on kidney function through increasing serum urea and creatinine concentrations and causing abnormal morphology of kidney tissue for rats treated with cisplatin during experimental period.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"86-95"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41622131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Falfushynska, O. Horyn, D. Poznansky, D. Osadchuk, T. Savchyn, T. I. Krytskyi, L. Merva, S. Z. Hrabra
Diabetes mellitus is a metabolic disorder that enhances fracture risk and hinders bone formation. The aim of the present study was to evaluate the parameters of oxidative stress, metallothioneins (MTs), metabolic changes and cytotoxicity signs in blood of young men with (DTF group) and without (TF group) type 2 diabetes (T2D) mellitus who had a tibia fracture due to trauma in relation to specific markers of bone formation. The level of reactive oxygen species was determined using a rOS-sensitive fluorescent dye dihydrorhodamine, Dna fragmentation was detected with hoescht 33342 fluorescent dye and caspase-3 was assessed in terms of acetyl-asp-Glu-Val-asp p-nitroanilide. all other studied indices were determined by standard spectrometric methods. Our results revealed the significant effect of T2D on the bone healing. indeed, the indices variation in the DTF group were significantly deeper as compared to group TF. The bone fracture in both TF and DTF groups had led to a significant decrease in antioxidants activity and/or level and a consistent increase in signs of oxidative damage. The concentration of MTs was also altered by trauma, but ina group-specific manner: an increase was noted in TF patients after trauma while in diabetes group a decrease in MTs was observed. likewise, glutathione was strongly suppressed (by -64%) in DTF group. Tibia fracture provoked cytotoxicity which was manifested by increasing lactate dehydrogenase (lDH), cholinesterase and caspase-3 activity, the key effector of apoptosis in osteoclasts. The activity of alkaline phosphatase and total calcium increased only in TF group which demonstrated adequate remodelling process. The most prominent indices for groups splitting include rOS concentration, caspase 3, glutathione transferase and lDH activities mostly conjoint to DTF group. In sum, T2D impairs bone healing under condition of severe oxidative stress and cellular thiols depletion which result in an increase in apoptosis and Dna fragmentation. Our findings establish a biochemical link between increased oxidative stress and reduced bone markers and provide a rational for further studies investigating the role of proand antioxidants in bone healing.
{"title":"Oxidative stress and thiols depletion impair tibia fracture healing in young men with type 2 diabetes","authors":"H. Falfushynska, O. Horyn, D. Poznansky, D. Osadchuk, T. Savchyn, T. I. Krytskyi, L. Merva, S. Z. Hrabra","doi":"10.15407/ubj91.06.067","DOIUrl":"https://doi.org/10.15407/ubj91.06.067","url":null,"abstract":"Diabetes mellitus is a metabolic disorder that enhances fracture risk and hinders bone formation. The aim of the present study was to evaluate the parameters of oxidative stress, metallothioneins (MTs), metabolic changes and cytotoxicity signs in blood of young men with (DTF group) and without (TF group) type 2 diabetes (T2D) mellitus who had a tibia fracture due to trauma in relation to specific markers of bone formation. The level of reactive oxygen species was determined using a rOS-sensitive fluorescent dye dihydrorhodamine, Dna fragmentation was detected with hoescht 33342 fluorescent dye and caspase-3 was assessed in terms of acetyl-asp-Glu-Val-asp p-nitroanilide. all other studied indices were determined by standard spectrometric methods. Our results revealed the significant effect of T2D on the bone healing. indeed, the indices variation in the DTF group were significantly deeper as compared to group TF. The bone fracture in both TF and DTF groups had led to a significant decrease in antioxidants activity and/or level and a consistent increase in signs of oxidative damage. The concentration of MTs was also altered by trauma, but ina group-specific manner: an increase was noted in TF patients after trauma while in diabetes group a decrease in MTs was observed. likewise, glutathione was strongly suppressed (by -64%) in DTF group. Tibia fracture provoked cytotoxicity which was manifested by increasing lactate dehydrogenase (lDH), cholinesterase and caspase-3 activity, the key effector of apoptosis in osteoclasts. The activity of alkaline phosphatase and total calcium increased only in TF group which demonstrated adequate remodelling process. The most prominent indices for groups splitting include rOS concentration, caspase 3, glutathione transferase and lDH activities mostly conjoint to DTF group. In sum, T2D impairs bone healing under condition of severe oxidative stress and cellular thiols depletion which result in an increase in apoptosis and Dna fragmentation. Our findings establish a biochemical link between increased oxidative stress and reduced bone markers and provide a rational for further studies investigating the role of proand antioxidants in bone healing.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"13 3","pages":"67-78"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41262116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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