O. Yaremchuk, K. Posokhova, І. P. Kuzmak, M. Kulitska, I. Klishch, M. Korda
antiphospholipid syndrome (aPS) is an autoimmune disease characterized by the presence of antibodies to negatively charged membrane phospholipids (aPL). endothelial dysfunction is one of the most dangerous APs manifestations followed by thrombosis, placental insufficiency and often foetal death due to circulatory disorders in placenta blood vessels. It is established that synthesis and bioavailability of nitric oxide (NO) in the endothelium are impaired at aPS, but the role of NO system in pregnancy failure at this pathology remains ambiguous. The aim of this research was to estimate the indexes of the nitric oxide system in animals with an experimental antiphospholipid syndrome before pregnancy and on the 18th day of pregnancy, without treatment and under treatment with nitric oxide synthesis modulators (L-arginine and aminoguanidine). In the blood serum and liver of the BaLB/c mice with experimental aPS, the content of eNOS and iNOS– by eLISa and the level of NO2 – and NO3 – with the use of Gris reagent were determined before pregnancy and on the 18th day of pregnancy. the data obtained indicate the relative inefficient No production by eNos and NO hyperproduction by iNOS in the blood serum and liver of mice in the pathogenesis of experimental aPS. Thus, in mice with aPS before pregnancy and on the 18th day of the pregnancy, the eNOS content and NO2 – level were decreased while the iNOS content and NO3 – level were increased compared to the indexes in the control animal group. L-arginine administration to the animals with aPS at the follow-up periods resulted in an increased eNOS content and NO2 –, NO3 – levels in blood serum and liver with the simultaneous decrease in iNOS content in the liver as compared to indexes in untreated mice with aPS. The combined use of L-arginine and selective iNos inhibitor aminoguanidine caused a significant increase in eNos content and a decrease in iNOS content followed by normalization of NO2 – and NO3 – levels in blood and liver of mice with experimental aPS before pregnancy and on the 18th day of pregnancy compared to untreated mice with aPS.
{"title":"Indexes of nitric oxide system in experimental antiphospholipid syndrome","authors":"O. Yaremchuk, K. Posokhova, І. P. Kuzmak, M. Kulitska, I. Klishch, M. Korda","doi":"10.15407/ubj92.01.075","DOIUrl":"https://doi.org/10.15407/ubj92.01.075","url":null,"abstract":"antiphospholipid syndrome (aPS) is an autoimmune disease characterized by the presence of antibodies to negatively charged membrane phospholipids (aPL). endothelial dysfunction is one of the most dangerous APs manifestations followed by thrombosis, placental insufficiency and often foetal death due to circulatory disorders in placenta blood vessels. It is established that synthesis and bioavailability of nitric oxide (NO) in the endothelium are impaired at aPS, but the role of NO system in pregnancy failure at this pathology remains ambiguous. The aim of this research was to estimate the indexes of the nitric oxide system in animals with an experimental antiphospholipid syndrome before pregnancy and on the 18th day of pregnancy, without treatment and under treatment with nitric oxide synthesis modulators (L-arginine and aminoguanidine). In the blood serum and liver of the BaLB/c mice with experimental aPS, the content of eNOS and iNOS– by eLISa and the level of NO2 – and NO3 – with the use of Gris reagent were determined before pregnancy and on the 18th day of pregnancy. the data obtained indicate the relative inefficient No production by eNos and NO hyperproduction by iNOS in the blood serum and liver of mice in the pathogenesis of experimental aPS. Thus, in mice with aPS before pregnancy and on the 18th day of the pregnancy, the eNOS content and NO2 – level were decreased while the iNOS content and NO3 – level were increased compared to the indexes in the control animal group. L-arginine administration to the animals with aPS at the follow-up periods resulted in an increased eNOS content and NO2 –, NO3 – levels in blood serum and liver with the simultaneous decrease in iNOS content in the liver as compared to indexes in untreated mice with aPS. The combined use of L-arginine and selective iNos inhibitor aminoguanidine caused a significant increase in eNos content and a decrease in iNOS content followed by normalization of NO2 – and NO3 – levels in blood and liver of mice with experimental aPS before pregnancy and on the 18th day of pregnancy compared to untreated mice with aPS.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"75-83"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43792376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Nobel laureates’ contributions to the study of carbohydrate metabolism and its regulation. A. Harden, H. Euler-Chelpin, C. F. Cori, G. T. Cori, E. Sutherland, L. F. Leloir, H. Krebs, F. Lipmann, P. Mitchell","authors":"R. P. Vynogradova, V. M. Danilova, S. Komisarenko","doi":"10.15407/ubj92.01.136","DOIUrl":"https://doi.org/10.15407/ubj92.01.136","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"136-163"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43765973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
the role of free radical oxidation in the increase of mitochondrial membranes permeability in organs which are not involved in oncogenesis and the development of the methods for preventing mitochondria dysfunction remain topical problems. In this work, the interconnection of lipid peroxidation (LPO) in liver mitochondrial fraction with the processes of mitochondrial swelling and cytochrome с release to the cytosol under separate and combined administration of ω-3 polyunsaturated fatty acids (PUFAs) and retinol acetate (vitamin a acetate) to rats with transplanted Guerin’s carcinoma was studied. During the intensive tumor growth (14 days) the increase of superoxide radical generation and the content of primary (triene conjugates, tc), secondary (ketodienes and coupled trienes, CD+CT) and terminal (Schiff bases) lipid peroxidation products in the mitochondrial fraction of tumor-bearing rats was detected, which contributed to the mitochondrial swelling and cytochrome с release to the cytosol. Separate administration of ω-3 PUFAs to tumor-bearing rats decreased both free radical processes in mitochondrial fraction and mitochondrial swelling. Separate administration of retinol acetate in a high dose (3000 IU/kg of body weight) intensified free radical processes in the mitochondrial fraction of tumor-bearing rats, while administration of retinol acetate in a physiological dose (30 IU/kg of body weight) did not lead to changes compared to tumor-bearing rats that did not receive the drug. The prooxidant effects of retinoid were partially eliminated in the case of combined administration with ω-3 PUFA.
{"title":"Free radical oxidation in liver mitochondria of tumor-bearing rats and its correction by essential lipophilic nutrients","authors":"O. Ketsa, M. Marchenko","doi":"10.15407/ubj92.01.127","DOIUrl":"https://doi.org/10.15407/ubj92.01.127","url":null,"abstract":"the role of free radical oxidation in the increase of mitochondrial membranes permeability in organs which are not involved in oncogenesis and the development of the methods for preventing mitochondria dysfunction remain topical problems. In this work, the interconnection of lipid peroxidation (LPO) in liver mitochondrial fraction with the processes of mitochondrial swelling and cytochrome с release to the cytosol under separate and combined administration of ω-3 polyunsaturated fatty acids (PUFAs) and retinol acetate (vitamin a acetate) to rats with transplanted Guerin’s carcinoma was studied. During the intensive tumor growth (14 days) the increase of superoxide radical generation and the content of primary (triene conjugates, tc), secondary (ketodienes and coupled trienes, CD+CT) and terminal (Schiff bases) lipid peroxidation products in the mitochondrial fraction of tumor-bearing rats was detected, which contributed to the mitochondrial swelling and cytochrome с release to the cytosol. Separate administration of ω-3 PUFAs to tumor-bearing rats decreased both free radical processes in mitochondrial fraction and mitochondrial swelling. Separate administration of retinol acetate in a high dose (3000 IU/kg of body weight) intensified free radical processes in the mitochondrial fraction of tumor-bearing rats, while administration of retinol acetate in a physiological dose (30 IU/kg of body weight) did not lead to changes compared to tumor-bearing rats that did not receive the drug. The prooxidant effects of retinoid were partially eliminated in the case of combined administration with ω-3 PUFA.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"127-134"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43653301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Korotkov, V. Nesterov, G. Belostotskaya, I. Brailovskaya, A. Novozhilov, C. V. Sobol
thallium is known to produce one of the most complex and serious patterns of toxicity, involving a wide range of human organs and tissues. the toxic impact on biologic organisms is linked especially to the ability of tl+ to disturb calcium homeostasis and to permeate easily the inner mitochondrial membrane (IMM). the aim of this work was to study the effects of Tl+ on intracellular Ca2+ dynamics in rat neonatal cardiomyocytes as well as on sodium penetrability of the IMM and tl+-induced mitochondrial permeability transition pore (MPtP) opening in isolated Ca2+-loaded rat heart mitochondria (RHM). The use of the fluorescent calcium indicator Fura 2 aM showed that tl+ induced calcium influx across the plasmatic membrane, resulting in calcium ([Ca]i) increase in the cytoplasm. this increase was even more pronounced in experiments with accelerating of tl+-transmembrane fluxes by nonactin. It was nevertheless abolished by the removal of extracellular Ca2+ ions, but was not inhibited by a calcium-channel blocker (nifedipine). tl+ did not release calcium from the intracellular stores. tl+ potentiated sodium permeability of the IMM because swelling of nonenergized rhM in medium containing tlNo3 and NaNo3 was enhanced at high tl + concentration. the calcium load of rhM induced MPtP opening which was accompanied by the increase of the swelling as well as the decrease of the inner membrane potential and of state 40 (basal) and state 3UDNP (2,4-dinitrophenol-uncoupled) respiration. These effects of Tl+ were suppressed by MPtP inhibitors (cyclosporine a, aDP and n-ethylmaleimide). the data obtained showed that tl+-stimulated influx of extracellular calcium into cardiomyocytes could cause calcium and sodium rhM overload, which lead to the MPtP opening, thus determining the sensitivity of heart muscle to thallium intoxication.
{"title":"Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes","authors":"S. Korotkov, V. Nesterov, G. Belostotskaya, I. Brailovskaya, A. Novozhilov, C. V. Sobol","doi":"10.15407/ubj92.01.041","DOIUrl":"https://doi.org/10.15407/ubj92.01.041","url":null,"abstract":"thallium is known to produce one of the most complex and serious patterns of toxicity, involving a wide range of human organs and tissues. the toxic impact on biologic organisms is linked especially to the ability of tl+ to disturb calcium homeostasis and to permeate easily the inner mitochondrial membrane (IMM). the aim of this work was to study the effects of Tl+ on intracellular Ca2+ dynamics in rat neonatal cardiomyocytes as well as on sodium penetrability of the IMM and tl+-induced mitochondrial permeability transition pore (MPtP) opening in isolated Ca2+-loaded rat heart mitochondria (RHM). The use of the fluorescent calcium indicator Fura 2 aM showed that tl+ induced calcium influx across the plasmatic membrane, resulting in calcium ([Ca]i) increase in the cytoplasm. this increase was even more pronounced in experiments with accelerating of tl+-transmembrane fluxes by nonactin. It was nevertheless abolished by the removal of extracellular Ca2+ ions, but was not inhibited by a calcium-channel blocker (nifedipine). tl+ did not release calcium from the intracellular stores. tl+ potentiated sodium permeability of the IMM because swelling of nonenergized rhM in medium containing tlNo3 and NaNo3 was enhanced at high tl + concentration. the calcium load of rhM induced MPtP opening which was accompanied by the increase of the swelling as well as the decrease of the inner membrane potential and of state 40 (basal) and state 3UDNP (2,4-dinitrophenol-uncoupled) respiration. These effects of Tl+ were suppressed by MPtP inhibitors (cyclosporine a, aDP and n-ethylmaleimide). the data obtained showed that tl+-stimulated influx of extracellular calcium into cardiomyocytes could cause calcium and sodium rhM overload, which lead to the MPtP opening, thus determining the sensitivity of heart muscle to thallium intoxication.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"41-55"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44976057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veklich To, Labyntseva Rd, Shkrabak Oa, O. Tsymbalyuk, R. Rodik, V. Kalchenko, S. Kosterin
The discovery of compounds that might modify myometrial contractility is an important area of researches. In our previous experiments, we found that some representatives of macrocyclic compounds family – calix[4]arenes – can modify the enzymatic and transport activity of membrane-bound cation-transport ATP hydrolases. The aim of this work was to study and compare the effect of calix[4]arene C-107 on the enzymatic activities of mg2+-dependent aTPases of the uterine smooth muscle, namely: ouabain-sensitive Na+,k+-aTPase, plasma membrane ca2+-independent “basal” mg2+-aTPase, aTPase of the actomyosin complex and myosin subfragment-1, with effect on the contractile activity of the myometrium. It was shown that calix[4]arene C-107 efficiently inhibited myometrium Na+,k+-aTPase (I50 = 54 ± 6 nm) selectively to other aTP-hydrolases of the plasma membrane and simultaneously activated the enzymatic activity of the myosin aTPase of smooth muscles (a50 = 9.6 ± 0.7 μM). Such reciprocal biochemical effects led to the stimulation of the smooth muscle contractile activity that was demonstrated by the tensometric method using different isolated smooth muscles. Calix[4]arene С-107 was shown to stimulate the increase of the tonic component of myometrium contractions induced by oxytocin, as well as contractions of the caecum muscles induced by high-potassium solution or acetylcholine, and to maintain increased tension for a long time. Thus, calix[4]arene C-107 is a prospective compound for enhancing the smooth muscle basal tone and/or contraction in case of hypotonic dysfunctions.
{"title":"Inhibition of Na(+),K(+)-ATPase and activation of myosin ATPase by calix[4]arene C-107 cause stimulation of isolated smooth muscle contractile activity","authors":"Veklich To, Labyntseva Rd, Shkrabak Oa, O. Tsymbalyuk, R. Rodik, V. Kalchenko, S. Kosterin","doi":"10.15407/ubj92.01.021","DOIUrl":"https://doi.org/10.15407/ubj92.01.021","url":null,"abstract":"The discovery of compounds that might modify myometrial contractility is an important area of researches. In our previous experiments, we found that some representatives of macrocyclic compounds family – calix[4]arenes – can modify the enzymatic and transport activity of membrane-bound cation-transport ATP hydrolases. The aim of this work was to study and compare the effect of calix[4]arene C-107 on the enzymatic activities of mg2+-dependent aTPases of the uterine smooth muscle, namely: ouabain-sensitive Na+,k+-aTPase, plasma membrane ca2+-independent “basal” mg2+-aTPase, aTPase of the actomyosin complex and myosin subfragment-1, with effect on the contractile activity of the myometrium. It was shown that calix[4]arene C-107 efficiently inhibited myometrium Na+,k+-aTPase (I50 = 54 ± 6 nm) selectively to other aTP-hydrolases of the plasma membrane and simultaneously activated the enzymatic activity of the myosin aTPase of smooth muscles (a50 = 9.6 ± 0.7 μM). Such reciprocal biochemical effects led to the stimulation of the smooth muscle contractile activity that was demonstrated by the tensometric method using different isolated smooth muscles. Calix[4]arene С-107 was shown to stimulate the increase of the tonic component of myometrium contractions induced by oxytocin, as well as contractions of the caecum muscles induced by high-potassium solution or acetylcholine, and to maintain increased tension for a long time. Thus, calix[4]arene C-107 is a prospective compound for enhancing the smooth muscle basal tone and/or contraction in case of hypotonic dysfunctions.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43929919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Moravej, S. M. Alavi, M. Azin, A. Salmanian, T. Biotechnology
Xanthan is a biopolymer produced by Xanthomonas bacteria which is widely used in many industries such as food and oil. In this work, three Xanthomonas strains (X. citri/NIGeB-88, X. citri/NIGeB-386 and X. citri/NIGeB-K37) were used to evaluate their industrial potential to produce xanthan gum in whey medium. Bacteria growth rate, viscosity, biomass, dry weigh of produced xanthan and β-galactosidase activity were studied during the fermentation process and the presence of β-galactosidase genes was assessed by PCR technique. Strain NIGeB-386 had the best ability to utilize lactose in the whey medium. The highest amount of xanthan production and viscosity were 22.7 g/l and 2066.6 mPa·s, respectively. The presence of six β-galactosidase genes in strains NIGEB-386 and NIGEB-K37 was confirmed. The pyruvate and acetyl contents in xanthan gum were 2.1 and 0.29 %, respectively. Fourier-transform infrared spectroscopy analysis determined the position of the functional groups in the structure of the fermentation product. In whey medium, the performance of both NIGEB-386 and NIGEB-K37 strains were better than the X. campestris. The findings showed that Xanthomonas citri/NIGeB-386 is suitable for industrial production of xanthan using cheese whey as a low-cost medium.
{"title":"Production and physicochemical characterization of xanthan gum by native lactose consuming isolates of Xanthomonas citri subsp. citri","authors":"R. Moravej, S. M. Alavi, M. Azin, A. Salmanian, T. Biotechnology","doi":"10.15407/ubj92.01.092","DOIUrl":"https://doi.org/10.15407/ubj92.01.092","url":null,"abstract":"Xanthan is a biopolymer produced by Xanthomonas bacteria which is widely used in many industries such as food and oil. In this work, three Xanthomonas strains (X. citri/NIGeB-88, X. citri/NIGeB-386 and X. citri/NIGeB-K37) were used to evaluate their industrial potential to produce xanthan gum in whey medium. Bacteria growth rate, viscosity, biomass, dry weigh of produced xanthan and β-galactosidase activity were studied during the fermentation process and the presence of β-galactosidase genes was assessed by PCR technique. Strain NIGeB-386 had the best ability to utilize lactose in the whey medium. The highest amount of xanthan production and viscosity were 22.7 g/l and 2066.6 mPa·s, respectively. The presence of six β-galactosidase genes in strains NIGEB-386 and NIGEB-K37 was confirmed. The pyruvate and acetyl contents in xanthan gum were 2.1 and 0.29 %, respectively. Fourier-transform infrared spectroscopy analysis determined the position of the functional groups in the structure of the fermentation product. In whey medium, the performance of both NIGEB-386 and NIGEB-K37 strains were better than the X. campestris. The findings showed that Xanthomonas citri/NIGeB-386 is suitable for industrial production of xanthan using cheese whey as a low-cost medium.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"92-102"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43447157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Sehin, S. Hnatush, O. Maslovska, A. Halushka, Y. Zaritska, Lviv Ukraine Feed Additives
Photolithotrophic sulfur bacteria are involved in biota functioning and have a biotechnological potential for bioremediation of contaminated environment, but the mechanisms of xenobiotics, in particular of heavy metal ions damaging action and the pathways of photolithotrophic bacteria adaptation under these conditions have not been established. In this work, the biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu ions were studied. C. limicola cells were incubated during one hour in buffer containing copper (II) sulfate in 0.05–0.5 mM concentrations and grown for 8 days in GSB medium. The content of Cu2+ in cells was estimated by atomic absorption spectroscopy. The activity of enzymes of antioxidant defense, photosynthetic pigments and glutathione content, indexes of lipids unsaturation and membrane viscosity as markers of membrane fluidity were estimated. It was shown that the response of green photosynthetic bacteria C. limicola to Cu2+ action varied depending on cations concentration. Under the influence of metal salt at 0.05 mM concentration, the activity of antioxidant enzymes, GSH/GSSG ratio, the content of photosynthetic pigments and membrane fluidity indexes were higher as compared with control. Under the increase of copper (II) sulfate concentration to 0.25 mM, the activity of antioxidant enzymes was lower compared to the response of the cells under the influence of 0.05 mM copper (II) and the GSSG content was increased. Under the influence of 0.5 mM copper (II) the indexes of membrane fluidity did not differ from the control, but superoxide dismutase and peroxidase activity inhibition and the further decrease of GSH/ GSSG ratio were observed followed by the highest Cu2+ cations accumulation in cells and significant decrease of bacteria biomass growth.
{"title":"Biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu(2+) action","authors":"T. Sehin, S. Hnatush, O. Maslovska, A. Halushka, Y. Zaritska, Lviv Ukraine Feed Additives","doi":"10.15407/ubj92.01.103","DOIUrl":"https://doi.org/10.15407/ubj92.01.103","url":null,"abstract":"Photolithotrophic sulfur bacteria are involved in biota functioning and have a biotechnological potential for bioremediation of contaminated environment, but the mechanisms of xenobiotics, in particular of heavy metal ions damaging action and the pathways of photolithotrophic bacteria adaptation under these conditions have not been established. In this work, the biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu ions were studied. C. limicola cells were incubated during one hour in buffer containing copper (II) sulfate in 0.05–0.5 mM concentrations and grown for 8 days in GSB medium. The content of Cu2+ in cells was estimated by atomic absorption spectroscopy. The activity of enzymes of antioxidant defense, photosynthetic pigments and glutathione content, indexes of lipids unsaturation and membrane viscosity as markers of membrane fluidity were estimated. It was shown that the response of green photosynthetic bacteria C. limicola to Cu2+ action varied depending on cations concentration. Under the influence of metal salt at 0.05 mM concentration, the activity of antioxidant enzymes, GSH/GSSG ratio, the content of photosynthetic pigments and membrane fluidity indexes were higher as compared with control. Under the increase of copper (II) sulfate concentration to 0.25 mM, the activity of antioxidant enzymes was lower compared to the response of the cells under the influence of 0.05 mM copper (II) and the GSSG content was increased. Under the influence of 0.5 mM copper (II) the indexes of membrane fluidity did not differ from the control, but superoxide dismutase and peroxidase activity inhibition and the further decrease of GSH/ GSSG ratio were observed followed by the highest Cu2+ cations accumulation in cells and significant decrease of bacteria biomass growth.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"103-112"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45860945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Fedota, L. Roschenyuk, T. Tyzhnenko, N. Puzik, V. Vorontsov, P. Ryzhko, Kharkiv Regional Clinical Skin
Methotrexate (MTX) is the immunosuppressive anti-inflammatory drug and the antagonist of the enzyme dihydrofolate reductase. Pharmacogenomic studies and clinical evidences suggest that altered response to MTX in patients with different diseases is associated with polymorphisms of genes that regulate folate metabolism. The purpose of the article was to analyze the methotrexate effect on the biochemical indices of psoriasis patients depending on methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms. Effects of two single-nucleotide polymorphisms, C677T and A1298C, were studied. An increase of alanine aminotransferase and aspartate aminotransferase activity above the normal level in the patients with both MTHFR gene polymorphisms after methotrexate intake was observed. In patients with CC, TT, CT genotypes for C677T polymorphism and AA genotype for A1298C polymorphism of MTHFR gene, significant differen ces in alphaamylase activity before and after treatment with methotrexate were detected. Analysis of the biochemical indices of patients with arthropathic and vulgaris psoriasis showed that the positive effect of MTX treatment could be associated with wild-type alleles in both polymorphisms of MTHFR gene, while the ineffectiveness of methotrexate was associated with the dihеterozygous genotype. The largest number of smokers was found within the ctaa genotype group (37.5%), while no smokers were observed within ttaa patients and most of CCAA patients. The data obtained testify the utility of the individual approach to the psoriasis patients therapy taking into account genetic background.
{"title":"Methotrexate effect on biochemical indices of psoriasis patients depends on MTHFR gene polymorphism","authors":"O. Fedota, L. Roschenyuk, T. Tyzhnenko, N. Puzik, V. Vorontsov, P. Ryzhko, Kharkiv Regional Clinical Skin","doi":"10.15407/ubj92.01.066","DOIUrl":"https://doi.org/10.15407/ubj92.01.066","url":null,"abstract":"Methotrexate (MTX) is the immunosuppressive anti-inflammatory drug and the antagonist of the enzyme dihydrofolate reductase. Pharmacogenomic studies and clinical evidences suggest that altered response to MTX in patients with different diseases is associated with polymorphisms of genes that regulate folate metabolism. The purpose of the article was to analyze the methotrexate effect on the biochemical indices of psoriasis patients depending on methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms. Effects of two single-nucleotide polymorphisms, C677T and A1298C, were studied. An increase of alanine aminotransferase and aspartate aminotransferase activity above the normal level in the patients with both MTHFR gene polymorphisms after methotrexate intake was observed. In patients with CC, TT, CT genotypes for C677T polymorphism and AA genotype for A1298C polymorphism of MTHFR gene, significant differen ces in alphaamylase activity before and after treatment with methotrexate were detected. Analysis of the biochemical indices of patients with arthropathic and vulgaris psoriasis showed that the positive effect of MTX treatment could be associated with wild-type alleles in both polymorphisms of MTHFR gene, while the ineffectiveness of methotrexate was associated with the dihеterozygous genotype. The largest number of smokers was found within the ctaa genotype group (37.5%), while no smokers were observed within ttaa patients and most of CCAA patients. The data obtained testify the utility of the individual approach to the psoriasis patients therapy taking into account genetic background.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"66-74"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48514329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. H. Moghadam, Z. Mehrabani, M. Amounajaf, S. Rahmanzadeh, F. Ghasemvand, A. S. Samghabadi, A. Nejadmoghaddam, E. Omidinia
1department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2department of clinical Biochemistry and laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; 3enzyme Technology lab., genetics & Metabolism research group, Pasteur Institute of Iran, Tehran, Iran; e-mail: saeed_r81@yahoo.com or 2000.spss@gmail.com
{"title":"Proline dehydrogenase (PRODH) gene polymorphisms and the risk of schizophrenia in Iranian populations","authors":"F. H. Moghadam, Z. Mehrabani, M. Amounajaf, S. Rahmanzadeh, F. Ghasemvand, A. S. Samghabadi, A. Nejadmoghaddam, E. Omidinia","doi":"10.15407/ubj92.01.012","DOIUrl":"https://doi.org/10.15407/ubj92.01.012","url":null,"abstract":"1department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2department of clinical Biochemistry and laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; 3enzyme Technology lab., genetics & Metabolism research group, Pasteur Institute of Iran, Tehran, Iran; e-mail: saeed_r81@yahoo.com or 2000.spss@gmail.com","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"12-20"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48178655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kopylchuk, I. Nykolaichuk, I. Lylyk, Chernivtsi Ukraine Bioresources
It is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocytes, but citrulline liver metabolism at these conditions remains incompletely clear. In this work, the content of citrulline in the rat liver mitochondrial and cytosolic fractions and the activity of citrulline-degrading enzymes – argininosuccinate synthase and argininosuccinate lyase were investigated. It was found that in the mitochondrial fraction a maximal reduction of the citrulline levels occurred after administration of acetaminophen toxic doses regardless of the protein amount in the ration, while in the cytosolic fraction the alimentary protein deficiency was a key factor in decreasing the activity of argininosuccinate synthase and arginino-succinate lyase. The data obtained indicated the disturbances of the urea cycle functioning and explained the decrease of l-arginine level in hepatocytes in conditions of acetaminophen-induced toxic injury against the background alimentary protein deficiency.
{"title":"Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency","authors":"H. Kopylchuk, I. Nykolaichuk, I. Lylyk, Chernivtsi Ukraine Bioresources","doi":"10.15407/ubj92.01.113","DOIUrl":"https://doi.org/10.15407/ubj92.01.113","url":null,"abstract":"It is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocytes, but citrulline liver metabolism at these conditions remains incompletely clear. In this work, the content of citrulline in the rat liver mitochondrial and cytosolic fractions and the activity of citrulline-degrading enzymes – argininosuccinate synthase and argininosuccinate lyase were investigated. It was found that in the mitochondrial fraction a maximal reduction of the citrulline levels occurred after administration of acetaminophen toxic doses regardless of the protein amount in the ration, while in the cytosolic fraction the alimentary protein deficiency was a key factor in decreasing the activity of argininosuccinate synthase and arginino-succinate lyase. The data obtained indicated the disturbances of the urea cycle functioning and explained the decrease of l-arginine level in hepatocytes in conditions of acetaminophen-induced toxic injury against the background alimentary protein deficiency.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"113-119"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46937987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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