Akopova Ov, L. Kolchinskaya, V. I. N. Kolchinskaya, A. Kolchinskaya, L. V. B. Kolchinskaya
{"title":"ATP-sensitive potassium transport in rat brain mitochondria is highly sensitive to mK(ATP) channels openers: a light scattering study","authors":"Akopova Ov, L. Kolchinskaya, V. I. N. Kolchinskaya, A. Kolchinskaya, L. V. B. Kolchinskaya","doi":"10.15407/ubj92.05.005","DOIUrl":"https://doi.org/10.15407/ubj92.05.005","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49141462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tautomeric hypothesis: to be or not to be? Quantum-mechanical verdict","authors":"O. Brovarets’, D. Hovorun","doi":"10.15407/ubj92.04.124","DOIUrl":"https://doi.org/10.15407/ubj92.04.124","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"124-126"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49518736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
recently enzyme preparations of microbial origin become increasingly important in different industries. Preparations of α-L-rhamnosidase are used in the pharmaceutical industry as well as in scientific work as a tool for analytical research. We have obtained purified α-L-rhamnosidase preparations from Penicillium tardum, eupenicillium erubescens and Cryptococcus albidus microorganism strains which are effective enzyme producers. the aim of the study was to estimate the ability of germanium coordination compounds to enhance enzyme catalytic activity. the effects of 11 heterometal mixed ligand tartrate (malate-)germanate compounds at 0.01 and 0.1% concentration on the activity of α-L-rhamnosidase preparations from Penicillium tardum IMV f-100074, eupenicillium erubescens and Cryptococcus albidus 1001 were studied at 0.5 and 24 h exposition. the inhibitory effect of [ni(bipy)3]4[{Ge2(oh)2(tart)2}3Cl2]·15h2 on P. tardum α-L-rhamnosidase was revealed. all studied compounds except [CuCl(phen)2][Ge(oh)(hMal)2] were shown to increase activity of P. tardum α-L-rhamnosidase at a longer term of exposition. activity of e. erubescens α-L-rhamnosidase was shown to be stimulated by d-metal cation-free compounds. C. albidus α-L-rhamnosidase occurred to be insensitive to all compounds studied.
{"title":"The influence of coordinative tartrate and malatogermanate compounds on the activity of ?-L-rhamnosidase preparations from Penicillium tardum, Eupenicillium erubescens and Cryptococcus albidus","authors":"О. V. Gudzenko, L. Varbanets, І. I. Seifullina","doi":"10.15407/ubj92.04.085","DOIUrl":"https://doi.org/10.15407/ubj92.04.085","url":null,"abstract":"recently enzyme preparations of microbial origin become increasingly important in different industries. Preparations of α-L-rhamnosidase are used in the pharmaceutical industry as well as in scientific work as a tool for analytical research. We have obtained purified α-L-rhamnosidase preparations from Penicillium tardum, eupenicillium erubescens and Cryptococcus albidus microorganism strains which are effective enzyme producers. the aim of the study was to estimate the ability of germanium coordination compounds to enhance enzyme catalytic activity. the effects of 11 heterometal mixed ligand tartrate (malate-)germanate compounds at 0.01 and 0.1% concentration on the activity of α-L-rhamnosidase preparations from Penicillium tardum IMV f-100074, eupenicillium erubescens and Cryptococcus albidus 1001 were studied at 0.5 and 24 h exposition. the inhibitory effect of [ni(bipy)3]4[{Ge2(oh)2(tart)2}3Cl2]·15h2 on P. tardum α-L-rhamnosidase was revealed. all studied compounds except [CuCl(phen)2][Ge(oh)(hMal)2] were shown to increase activity of P. tardum α-L-rhamnosidase at a longer term of exposition. activity of e. erubescens α-L-rhamnosidase was shown to be stimulated by d-metal cation-free compounds. C. albidus α-L-rhamnosidase occurred to be insensitive to all compounds studied.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"85-95"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45863010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the 20th century, DNa became a magnet, attracting representatives of various sciences. Prominent researchers competed among themselves to discover the structure of DNa and to explain the mechanisms that determine our “natural fate”, i.e., our heredity. an american chemist, biochemist, chemical engineer linus Pauling, a British physicist and molecular biologist maurice Wilkins, a British chemist, biophysicist, and X-ray crystallographer rosalind Franklin, an american geneticist, molecular biologist, zoologist James Watson, a British molecular biologist, biophysicist, and neuroscientist Francis Crick were among them. They searched for the scientific explanation for the enigma of life hidden in DNA. An accurate description of DNA double-helical structure belongs to James Watson and Francis Crick. However, the missing pieces of the puzzle were elaborated by Rosalind Franklin, who was not given enough credit for her dedicated scientific work. Unlike her, Francis Crick, James Watson, and maurice Wilkins were awarded the Nobel Prize in Physiology or Medicine 1962 for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material. Whatever the DNA story is, it shows that all great scientific discoveries are not made from scratch. The immense number of people have contributed to the development of science and literally every researcher stands on the shoulders of giants, while the idea itself is in the air. The discovery of the structure of DNA became a cornerstone for the new scientific paradigm – biology acquired a molecular and biochemical basis.
{"title":"Standing on the shoulders of giants: James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin and the birth of molecular biology","authors":"T. Danylova, S. Komisarenko","doi":"10.15407/ubj92.04.154","DOIUrl":"https://doi.org/10.15407/ubj92.04.154","url":null,"abstract":"In the 20th century, DNa became a magnet, attracting representatives of various sciences. Prominent researchers competed among themselves to discover the structure of DNa and to explain the mechanisms that determine our “natural fate”, i.e., our heredity. an american chemist, biochemist, chemical engineer linus Pauling, a British physicist and molecular biologist maurice Wilkins, a British chemist, biophysicist, and X-ray crystallographer rosalind Franklin, an american geneticist, molecular biologist, zoologist James Watson, a British molecular biologist, biophysicist, and neuroscientist Francis Crick were among them. They searched for the scientific explanation for the enigma of life hidden in DNA. An accurate description of DNA double-helical structure belongs to James Watson and Francis Crick. However, the missing pieces of the puzzle were elaborated by Rosalind Franklin, who was not given enough credit for her dedicated scientific work. Unlike her, Francis Crick, James Watson, and maurice Wilkins were awarded the Nobel Prize in Physiology or Medicine 1962 for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material. Whatever the DNA story is, it shows that all great scientific discoveries are not made from scratch. The immense number of people have contributed to the development of science and literally every researcher stands on the shoulders of giants, while the idea itself is in the air. The discovery of the structure of DNA became a cornerstone for the new scientific paradigm – biology acquired a molecular and biochemical basis.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"154-165"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43177271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The contribution of Nobel prize laureates to research of the protein structure: J. Sumner, J. Northrop, W. Stanley, L. Pauling, F. Sanger, M. Perutz, J. Kendrew","authors":"V. M. Danilova, R. P. Vynogradova, S. Komisarenko","doi":"10.15407/ubj92.04.127","DOIUrl":"https://doi.org/10.15407/ubj92.04.127","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"127-153"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43368051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corchorus olitorius is a species of shrub in the family malvaceae known to have rich sources of chemical compounds. the leaves of this plant are locally consumed as a vegetable and as a remedy in Africa, middle east and Southern Asia. this study was designed to evaluate the hypoglycemic and hypolipidemic effects of C. olitorius leaves sample given for 14 days as a food supplement to rats with alloxan-induced diabetes. Twenty one male albino rats weighing 150-200 g were randomly assigned into three groups: 1 – control rats; 2 – diabetic rats; 3 – diabetic rats fed with C. olitorius leaves as supplement. Phytochemical analysis of C. olitorius leaves ethanolic extract revealed the presence of alkaloids, anthraquinone, cardiac glycosides, saponins, tannins, phenols, phlobatannins and flavonoids. Diabetic rats fed with C. olitorius leaves as a supplement were shown to have significantly (P < 0.01) decreased plasma glucose, LDL-cholesterol, total cholesterol and triglycerides levels when compared with untreated diabetic rats. The findings from this study indicated that C. olitorius leaf possesses hypoglycemic and hypolipidemic properties.
{"title":"Hypoglycemic and hypolipidemic effects of Corchorus olitorius leaves as a food supplement on rats with alloxan-induced diabet","authors":"A. Nduka, A. Oluwatoyin, A. Temitope, O. Odutola","doi":"10.15407/ubj92.04.063","DOIUrl":"https://doi.org/10.15407/ubj92.04.063","url":null,"abstract":"Corchorus olitorius is a species of shrub in the family malvaceae known to have rich sources of chemical compounds. the leaves of this plant are locally consumed as a vegetable and as a remedy in Africa, middle east and Southern Asia. this study was designed to evaluate the hypoglycemic and hypolipidemic effects of C. olitorius leaves sample given for 14 days as a food supplement to rats with alloxan-induced diabetes. Twenty one male albino rats weighing 150-200 g were randomly assigned into three groups: 1 – control rats; 2 – diabetic rats; 3 – diabetic rats fed with C. olitorius leaves as supplement. Phytochemical analysis of C. olitorius leaves ethanolic extract revealed the presence of alkaloids, anthraquinone, cardiac glycosides, saponins, tannins, phenols, phlobatannins and flavonoids. Diabetic rats fed with C. olitorius leaves as a supplement were shown to have significantly (P < 0.01) decreased plasma glucose, LDL-cholesterol, total cholesterol and triglycerides levels when compared with untreated diabetic rats. The findings from this study indicated that C. olitorius leaf possesses hypoglycemic and hypolipidemic properties.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"63-69"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43490725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant adaptation to climate conditions of certain territories has emerged within the course of evolution, shows at all organizational levels from morphological-anatomical to biochemical and is embedded into the plant genes. Survival of plants in such conditions as rapid temperature drops and rises in the range of 20 °C or more depends on their biochemical defense system's ability to quickly respond to such stress, as well as on the plant's structural features. Therefore, our goal was to analyze changes of biochemical parameters under conditions of abrupt hyperthermia in four species of Crassula linne genus and to establish the connection between their anatomical and morphological features and the peculiarities of the biochemical reactions. Plants of Crassula brevifolia Harvey, Crassula lanuliginosa Harvey, Crassula muscosa Linne and Сrassula perfoliata var. minor (haworth) G.d. rowley species were held in air thermostats at 40 °C and 50 °C for 3 h, the control temperature being 26 °C. Stress response was analyzed by malondialdehyde content, superoxide dismutase and peroxidase activity and pigments content. additionally, anatomical structure of the leaves was investigated. Antioxidant response to short-term high temperature varied in different species of the Crassula genus by its directionality and intensity, and depended on the anatomical features of the plant. The additional protective mechanisms were involved in the least heat-resistant plants, such as increased carotenoids and flavonoids contents. More powerful SOD and peroxidase activities under rapid heating in plants with more effective protection at the anatomical level were showed.
{"title":"Crassula genus plants response to temperature stress depends on anatomical structure and antioxidant system","authors":"N. Nuzhyna, M. Gaidarzhy, A. Holubenko","doi":"10.15407/ubj92.04.111","DOIUrl":"https://doi.org/10.15407/ubj92.04.111","url":null,"abstract":"Plant adaptation to climate conditions of certain territories has emerged within the course of evolution, shows at all organizational levels from morphological-anatomical to biochemical and is embedded into the plant genes. Survival of plants in such conditions as rapid temperature drops and rises in the range of 20 °C or more depends on their biochemical defense system's ability to quickly respond to such stress, as well as on the plant's structural features. Therefore, our goal was to analyze changes of biochemical parameters under conditions of abrupt hyperthermia in four species of Crassula linne genus and to establish the connection between their anatomical and morphological features and the peculiarities of the biochemical reactions. Plants of Crassula brevifolia Harvey, Crassula lanuliginosa Harvey, Crassula muscosa Linne and Сrassula perfoliata var. minor (haworth) G.d. rowley species were held in air thermostats at 40 °C and 50 °C for 3 h, the control temperature being 26 °C. Stress response was analyzed by malondialdehyde content, superoxide dismutase and peroxidase activity and pigments content. additionally, anatomical structure of the leaves was investigated. Antioxidant response to short-term high temperature varied in different species of the Crassula genus by its directionality and intensity, and depended on the anatomical features of the plant. The additional protective mechanisms were involved in the least heat-resistant plants, such as increased carotenoids and flavonoids contents. More powerful SOD and peroxidase activities under rapid heating in plants with more effective protection at the anatomical level were showed.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"111-123"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43634546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
the effect of two water-soluble sulfonamides: aromatic 4-aminobenzenesulfonamide (Sa) and aliphatic trifluoromethylsulfonamide (tFmSa) on the latent and stimulated atPase activity of the catalytic part of the thylakoid atP synthase isolated from spinach (Spinacia oleracea l.) chloroplasts was studied. three methods of atPase activity stimulation were applied in the work: brief heating of the enzyme at 62 °С; incubation with 20% methanol; 25 mm sodium sulfite adding to the reaction medium. tFmSa effectively inhibited the atPase activity of the enzyme at all types of activation. i50 (tFmSa) value for was found to be about 5 μm in the case of enzyme heat treatment and 1 μm in the case of enzyme activation with sulfite or methanol. Sa did not affect atPase activity at stimulation with sulfite and inhibited with i50 about 50 μm at stimulation by heating or methanol. no effect of Sa on latent enzyme activity was observed, while tFmSa was shown to activate latent atPase by about 6 times. the data obtained showed that both sulfonamide compounds, known as carbonic anhydrase inhibitors, are able to modify the activity of chloroplast atPase.
{"title":"Sulfonamides influence on the activity of thylakoid ATPase isolated from spinach chloroplasts","authors":"Е. K. Zolotareva, A. P. Khomochkin, O. B. Onoiko","doi":"10.15407/ubj92.04.096","DOIUrl":"https://doi.org/10.15407/ubj92.04.096","url":null,"abstract":"the effect of two water-soluble sulfonamides: aromatic 4-aminobenzenesulfonamide (Sa) and aliphatic trifluoromethylsulfonamide (tFmSa) on the latent and stimulated atPase activity of the catalytic part of the thylakoid atP synthase isolated from spinach (Spinacia oleracea l.) chloroplasts was studied. three methods of atPase activity stimulation were applied in the work: brief heating of the enzyme at 62 °С; incubation with 20% methanol; 25 mm sodium sulfite adding to the reaction medium. tFmSa effectively inhibited the atPase activity of the enzyme at all types of activation. i50 (tFmSa) value for was found to be about 5 μm in the case of enzyme heat treatment and 1 μm in the case of enzyme activation with sulfite or methanol. Sa did not affect atPase activity at stimulation with sulfite and inhibited with i50 about 50 μm at stimulation by heating or methanol. no effect of Sa on latent enzyme activity was observed, while tFmSa was shown to activate latent atPase by about 6 times. the data obtained showed that both sulfonamide compounds, known as carbonic anhydrase inhibitors, are able to modify the activity of chloroplast atPase.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42004200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial Ca2+ overload may trigger the opening of mitochondrial permeability transition pore (mPTP) and its prolonged activation leads to cell death. aTP synthase is considered as a possible molecular component of the pore. The aim of this study was to investigate the state of oxidative phosphorylation at Ca2+induced activation of mPTP in permeabilized hepatocytes. Hepatocytes were isolated by two-stage Seglen method. Permeabilization was performed using digitonin. Oxygen consumption rate was measured with Clark electrode. Oxidative phosphorylation was determined as the ratio of the aDP-stimulated respiration and substrate-stimulated respiration rates (aDP/S). It was established that increasing of Ca2+ concentration in the medium inhibited oligomycin effects and suppressed ADPand FCCP-stimulated respiration upon succinate or glutamate, pyruvate and malate mixture oxidation. The mPTP inhibitor cyclosporin a did not directly affect respiration and oxidative phosphorylation after elevation of Ca2+ concentration and mPTP activation. When cyclosporine a was added before increasing Ca2+ concentration, the electron transport chain function (FCCP-stimulated respiration) was not impaired while the partial disruption of oxidative phosphorylation (aDP-stimulated respiration) was observed only upon succinate oxidation. The results obtained showed that inhibition of oxidative phosphorylation was the primary event in mPTP activation, possibly due to the involvement of ATP synthase in pore opening. In the case of NAD-dependent substrates oxidation that effect was stronger and faster than at succinate oxidation, due to the lower mitochondria energization.
{"title":"mPTP opening differently affects electron transport chain and oxidative phosphorylation at succinate and NAD-dependent substrates oxidation in permeabilized rat hepatocytes","authors":"H. Mazur, V. M. Merlavsky, B. O. Manko, V. Manko","doi":"10.15407/ubj92.04.014","DOIUrl":"https://doi.org/10.15407/ubj92.04.014","url":null,"abstract":"Mitochondrial Ca2+ overload may trigger the opening of mitochondrial permeability transition pore (mPTP) and its prolonged activation leads to cell death. aTP synthase is considered as a possible molecular component of the pore. The aim of this study was to investigate the state of oxidative phosphorylation at Ca2+induced activation of mPTP in permeabilized hepatocytes. Hepatocytes were isolated by two-stage Seglen method. Permeabilization was performed using digitonin. Oxygen consumption rate was measured with Clark electrode. Oxidative phosphorylation was determined as the ratio of the aDP-stimulated respiration and substrate-stimulated respiration rates (aDP/S). It was established that increasing of Ca2+ concentration in the medium inhibited oligomycin effects and suppressed ADPand FCCP-stimulated respiration upon succinate or glutamate, pyruvate and malate mixture oxidation. The mPTP inhibitor cyclosporin a did not directly affect respiration and oxidative phosphorylation after elevation of Ca2+ concentration and mPTP activation. When cyclosporine a was added before increasing Ca2+ concentration, the electron transport chain function (FCCP-stimulated respiration) was not impaired while the partial disruption of oxidative phosphorylation (aDP-stimulated respiration) was observed only upon succinate oxidation. The results obtained showed that inhibition of oxidative phosphorylation was the primary event in mPTP activation, possibly due to the involvement of ATP synthase in pore opening. In the case of NAD-dependent substrates oxidation that effect was stronger and faster than at succinate oxidation, due to the lower mitochondria energization.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"14-23"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41611517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gahramanova, I. Khalilova, A. Omarov, Y. Susak, M. Rudyk, L. Skivka
Herbal preparations are widely used in the complementary treatment of inflammatory diseases including hepatobiliary disorders, among which chronic cholecystitis is one of the most common. This study was aimed to investigate the effect of the water extract from polyherbal composition consisting of eight medicinal plants (Helichrysum arenarium, Mentha piperita, Calendula officinalis, Taraxacum officinale, Polygonum aviculare, Matricaria chamomilla, Portulaca oleracea, hypericum perforatum) on biochemical and haematologic indices as well as on immune reactivity parameters in patients with chronic cholecystitis. It was found that consumption of polyherbal tea during 45 days was followed by normalization of liver biochemical indices (serum level of cholesterol, alanine aminotransferase and aspartate aminotransferase activity, total and unconjugated bilirubin levels and diminution of hematological (leukocytosis, increased erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio) and immunological (increased serum level of pro-inflammatory cytokines) signs of systemic inflammation in patients with hepatobiliary disorder. The results of the research showed that the studied polygerbal composition can be recommended as a component of the complementary therapy for patients with chronic cholecystitis.
{"title":"Anti-inflammatory and hepatoprotective effects of polyherbal composition in patients with chronic cholecystitis","authors":"M. Gahramanova, I. Khalilova, A. Omarov, Y. Susak, M. Rudyk, L. Skivka","doi":"10.15407/ubj92.04.077","DOIUrl":"https://doi.org/10.15407/ubj92.04.077","url":null,"abstract":"Herbal preparations are widely used in the complementary treatment of inflammatory diseases including hepatobiliary disorders, among which chronic cholecystitis is one of the most common. This study was aimed to investigate the effect of the water extract from polyherbal composition consisting of eight medicinal plants (Helichrysum arenarium, Mentha piperita, Calendula officinalis, Taraxacum officinale, Polygonum aviculare, Matricaria chamomilla, Portulaca oleracea, hypericum perforatum) on biochemical and haematologic indices as well as on immune reactivity parameters in patients with chronic cholecystitis. It was found that consumption of polyherbal tea during 45 days was followed by normalization of liver biochemical indices (serum level of cholesterol, alanine aminotransferase and aspartate aminotransferase activity, total and unconjugated bilirubin levels and diminution of hematological (leukocytosis, increased erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio) and immunological (increased serum level of pro-inflammatory cytokines) signs of systemic inflammation in patients with hepatobiliary disorder. The results of the research showed that the studied polygerbal composition can be recommended as a component of the complementary therapy for patients with chronic cholecystitis.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"77-84"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43902852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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