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Molecular detection of CYP51 genes in Aspergillus fumigatus 烟曲霉CYP51基因的分子检测
Pub Date : 2019-04-25 DOI: 10.32792/utq/utj/vol14/1/9
Amal J. Kadhim, Yass K. Abbass, Khwam, R. Hussein
Triazoles are the mainstay for aspergillosis treatment. However, azole resistance is an emerging problem reported worldwide in Aspergillus infection mainly caused by A.fumigatus . Increase azole resistance in A.fumigatus has reported with treatment failure and become a significant challenge in effective management of aspergillosis. In the current study , A.fumigatus isolates were resistant to itraconazole and ketonazole with percentage of 36% and 44% respectively. The conventional Polymerase Chain Reaction (PCR) was used to confirm antifungal resistance by detecting the presence of CYP51A and CYP51B genes. The results of using this technique showed that A. fumigatus isolates were positive to these genes with 100% percentage. Our study revealed that the CYP51 genes are predominant in azole resistance isolates. Furthermore, PCR was proven to be highly effective method for identifying these genes.
三唑类药物是治疗曲霉病的主要药物。然而,在全球范围内,主要由烟曲霉引起的曲霉感染中,唑耐药性是一个新出现的问题。据报道,烟曲霉对唑的耐药性增加,治疗失败,成为有效管理曲霉病的重大挑战。本研究烟曲霉对伊曲康唑和酮唑的耐药率分别为36%和44%。通过检测CYP51A和CYP51B基因的存在,采用传统的聚合酶链反应(PCR)来证实抗真菌耐药性。结果表明,烟曲霉分离物对这些基因的阳性检出率为100%。我们的研究表明,CYP51基因在唑抗性分离株中占主导地位。此外,PCR被证明是鉴定这些基因的有效方法。
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引用次数: 0
Effect of Bromocriptine on Anthropometric, Metabolic and Inflammatory Parameters in Obese Women 溴隐亭对肥胖女性人体测量、代谢和炎症参数的影响
Pub Date : 2019-04-24 DOI: 10.32792//utq/utjmed/16/2/1
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引用次数: 0
Evaluation the levels of thyroid hormones in pregnant women with phospholipid syndrome 评估患有磷脂综合征的孕妇甲状腺激素水平
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/4/8
Shaymaa Z. Al-Rumaidh
Miscarriage in the early and late stages of pregnancy is a common problem in women with the anti-phospholipids syndrome. The current study aimed to determine the relationship between the syndrome of antibodies to phospholipid and thyroid hormones among pregnant women in the city of Nasiriyah. Blood samples were collected from (100) pregnant women with recurrent miscarriages due to phospholipid syndrome, the average aged (20-30) and (30-40) years. The results showed a significant increase in T3 and TSH hormones between ages (20-30), (30-40) years compared to the control group, as well as a significant increase in T4 hormone in pregnant women aged (20-30) while the ages group of (30-40) did not notice a significant change in the level of hormone T4 compared with the control group. We conclude that there is a relationship between thyroid hormones and autoimmune diseases.
妊娠早期和晚期流产是患有抗磷脂综合征妇女的常见问题。目前的研究旨在确定纳西里耶市孕妇中磷脂抗体综合征和甲状腺激素之间的关系。采集了100例因磷脂综合征而复发性流产的孕妇的血液样本,平均年龄为20-30岁和30-40岁。结果显示,与对照组相比,(20-30岁)、(30-40岁)年龄段的孕妇T3、TSH水平明显升高,(20-30岁)年龄段的孕妇T4水平明显升高,(30-40岁)年龄段的孕妇T4水平没有明显变化。我们得出结论,甲状腺激素与自身免疫性疾病之间存在关系。
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引用次数: 0
Plasticity investigation of Medium Carbon Steel ( AISI CK45 ) treated by Quenching media . 淬火介质处理中碳钢(AISI CK45)的塑性研究
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/1/6
Ayad Abdulameer Abdulhussein
This paper goals to investigate the plasticity of Medium Carbon Steel (AISI CK45) treated by three of Quenching ( Salty . Acid and Base ) media , the sample was selected with length (100 mm) and (10 mm) diameter  , has been prepared in the form of a shaft , (24) samples were immersed in different media for (100) hours, the conclusion that the quenching process of ( AISI CK 45 ) at salty (NaCl) media at a concentration of (5%) will give a high plasticity .
本文的目的是研究中碳钢(AISI CK45)经三种淬火(含盐淬火)处理后的塑性。(24)试样分别在不同的介质中浸泡(100)小时,得出(AISI CK 45)在浓度为(5%)的盐(NaCl)介质中淬火处理会获得较高的塑性的结论。
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引用次数: 2
ELISA and ICT Techniqes in the detection of anti- Rubella virus Antibodies in Aborted women in Al- Nasiriyah city, Thi-Qar, Iraq ELISA和ICT技术检测伊拉克Al- Nasiriyah市流产妇女抗风疹病毒抗体的研究
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol12/4/4
A. Fayad, I. N. Abid
A total of 200 serum samples were collected from Bent Al-Huda hospital in Al -Nasiriyah city, Thi- Qar province, Iraq. These samples were collected from aborted women in different ages and cases to detect of Rubella virus by Enzyme Linked Immuno Sorbent Assay (ELISA) and Immuno chromatography (ICT) techniques.   It was founded from the recent study 162 (81%) of serum samples were positive by IgG ELISA, and 105 (52.5%) were positive by IgG with ICT technique. All serum samples. were registered negative results to IgM anti- rubella virus by two techniques.   The results of ELISA technique were appeared the higher rate (97.95%) for anti-Rubella virus antibodies was observed in second age group ((21-30)years) and a no significant differences were observed in the seropositivity concerning to age groups(P value:>0.05). In ICT, the higher rate (85.71%) of anti-Rubella virus antibodies was observed in the second group of ages, with the presence of significant among the age groups(P value: 0.05), while 90.9% of seropositivity fetal death cases was resulted with ICT technique, and were founded highly significant differences among the rates of seropositivity against rubella virus(P value:< 0.01). The sensitivity and specificity were registered 95% and 61% respectively with ELISA test, while the lower sensitivity and sensitivity values were with ICT test, they were 85% and 57% respectively, and the statistical analysis was appeared the existence of significant differences (P value:< 0.05) between the two tests.
在伊拉克Thi- Qar省Al- nasiriyah市的Bent Al- huda医院共收集了200份血清样本。采用酶联免疫吸附试验(ELISA)和免疫层析(ICT)技术对不同年龄和病例的流产妇女进行风疹病毒检测。结果发现,162份血清IgG ELISA检测阳性(81%),105份血清IgG ICT检测阳性(52.5%)。所有血清样本。两种方法对抗风疹病毒IgM抗体检测结果均为阴性。ELISA检测结果显示,2龄组(21 ~ 30岁)风疹病毒抗体阳性率最高(97.95%),各年龄组间血清阳性率差异无统计学意义(P值:0.05)。ICT组抗风疹病毒抗体检出率(85.71%)高于第二组,各年龄组间差异有统计学意义(P值:0.05);ICT组血清风疹病毒抗体检出率为90.9%,两组间风疹病毒血清检出率差异有极显著性(P值< 0.01)。ELISA检测的灵敏度和特异度分别为95%和61%,ICT检测的灵敏度和特异度较低,分别为85%和57%,两种检测的差异有统计学意义(P值< 0.05)。
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引用次数: 1
STUDY THE IMPACT OF ANTIVENOM OF Echis carinatus SNAKE ON BLOOD PARAMETER, ENZYMES AND SEX HORMONES PARAMETERS IN WISTER AlBINO MALE RATS 摘要研究蛇抗蛇毒液对白化雄性大鼠血液指标、酶及性激素指标的影响
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/1/4
B. R. Ali, S. Enayah, K. Al-Fartosi
In the current study the authors aimed to investigate the effect of anti-venom of Echis carinatus on hematological and hormones parameters as well as explore its influence on liver enzymes of male rats. Mature adult rats were divided into three groups 6 animals for each group. IP injected method was used. First group (Control group) injected with normal saline (0.9%Nacl), Two group injected with (0.25ml/kg) of anti-venom for two times, and the third group injected with (0.5ml/kg) of antivenom for two times. Animals killed within 24 h. The outcome of the current study showed that there are statistically different in RBC, MCH, MCHC, PCV, MCV, total WBC, lymphocytes, monocytes and granulocytes among second and third groups compared with control group (P≤0.05). In addition, the results show a significant increasing the number of platelets and liver enzymes ALT, AST, ALP in the highly-treated group comparing with the control. Also, level of FSH, LH and testosterone likewise increased in the treated groups comparing with control. Anti-venom reduced sperm count in male rats.
本研究旨在探讨鱼鳗抗蛇毒对雄性大鼠血液学和激素参数的影响,以及对肝酶的影响。将成年大鼠分为3组,每组6只。采用IP注射法。第一组(对照组)注射生理盐水(0.9%Nacl),第二组注射(0.25ml/kg)抗蛇毒血清2次,第三组注射(0.5ml/kg)抗蛇毒血清2次。本研究结果显示,第二组和第三组的红细胞、MCH、MCHC、PCV、MCV、总白细胞、淋巴细胞、单核细胞和粒细胞与对照组相比有统计学差异(P≤0.05)。此外,结果显示,与对照组相比,高剂量治疗组血小板数量和肝酶ALT、AST、ALP显著增加。此外,与对照组相比,治疗组的FSH、LH和睾酮水平也同样升高。抗蛇毒血清减少了雄性大鼠的精子数量。
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引用次数: 0
Double Slope Solar Still: Study, Design and Improve Productivity 双斜面太阳能蒸馏器:研究、设计和提高生产率
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/1/11
Jamal S. Abd Al Rukabie
Two identical of double slope solar stills with same specifications were designed to study and improve their productivity to produce fresh water (drinking water) from salty water especially in the regions where the fresh water is low or unavailable such as south of IRAQ. One of the two solar stills was subjected to modifications in order to increase the productivity of drinking water and to compare with non-modified solar still. Covering the basin of solar still with black piece of cloth increase the productivity up to( 22.35 percent). By adding charcoal powder on top of black piece of cloth the productivity was increased( 34.21 percent). However, the productivity was increased to (16.69 percent) when two reflections mirror was used. Also, thermal efficiency of solar still was calculated. The highest value of( 34.13 percent) was achieved when modifications applied. In this project, the chemical and physical properties of the water before and after distillation were analyzed. The double slope solar still produced fresh and drinkable water. Keywords : Double slope solar still , black paint , Charcoal powder , black cloth , Design , Improve Productivity.
设计了两个规格相同的双斜面太阳能蒸馏器,以研究和提高其从咸水中生产淡水(饮用水)的生产力,特别是在伊拉克南部等淡水较少或无法获得的地区。两个太阳能蒸馏器中的一个进行了修改,以提高饮用水的生产率,并与未修改的太阳能蒸馏器进行比较。用黑布覆盖太阳能蒸馏器盆,提高生产率达22.35%。在黑布上加炭粉可提高生产效率(34.21%)。而使用双反射镜时,效率提高到16.69%。并对太阳能蒸馏器的热效率进行了计算。当进行修改时达到最高值(34.13%)。本课题对蒸馏前后的水进行了理化性质分析。双坡太阳能仍能生产出新鲜的饮用水。关键词:双斜面太阳能蒸馏器,黑漆,炭粉,黑布,设计,提高生产率。
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引用次数: 2
Study of Dielectric properties for porous silicon prepared by Anodization 阳极氧化法制备多孔硅的介电性能研究
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/4/4
Salah M. Abd Ulaziz
The nanostructure for porous silicon (PS) films is produced by anodization of p-type silicon chip with current consistency (15 mA/cm2), etching time  about 10 min and HF concentration (32%) to  the formation nanosized pore order with a dimension of around few hundreds nanometric. The films were featured by the measurement of atomic force microscopy (AFM) in the Baghdad university, X-Ray diffraction (XRD) and FTIR spectroscopy characteristics in the technological university. I am having appraising crystallites size from XRD about nanoscale for porous silicon and AFM proves the nanometric size. Chemical fictionalizations through the electrochemical etching clear on superficies chemical structure of PS. The etching possess disproportionate microstructures that include Si-H clusters in (Si3-SiH),   sparse in amorphous silica matrix and Si-H2 scissor mode. From the FTIR testing appeared that the Si suspending bonds of the as- produced PS layer have massive quantities of Hydrogen to form weak Si–H bonds related to Si–H stretch (Si3-SiH) and Si-H stretch (Si2-SiH). The AC electrical properties were measured with LCR meter analyzer the frequency between 50 Hz and 5 MHz. for capacitance, dielectric and loss tangent (tan δD) to balk silicon and porous silicon have been done in the ministry of science and technology.
多孔硅(PS)薄膜的纳米结构是在电流浓度为15 mA/cm2、蚀刻时间约为10 min、HF浓度为32%的p型硅片阳极氧化后形成的,孔径约为几百纳米。用巴格达大学的原子力显微镜(AFM)、工业大学的x射线衍射(XRD)和红外光谱(FTIR)对薄膜进行了表征。我对多孔硅进行了纳米尺度的XRD和AFM鉴定。通过电化学刻蚀的化学模拟,可以清楚地看到PS的表面化学结构。刻蚀具有不成比例的微观结构,包括(Si3-SiH)中的Si-H簇,无定形二氧化硅基体中的稀疏和Si-H2剪刀模式。FTIR测试表明,制备的PS层的Si悬浮键中含有大量的氢,形成与Si- h拉伸(Si3-SiH)和Si- h拉伸(Si2-SiH)有关的弱Si- h键。在50hz ~ 5mhz的频率范围内,用LCR仪表分析仪测量了材料的交流电性能。科技部对阻硅和多孔硅的电容、介电和损耗正切(tan δD)进行了测定。
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引用次数: 0
The Effect of Load Resistance on uncontrolled Rectifier Performance 负载电阻对非控制整流器性能的影响
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol13/3/1
A. Tuaimah
This paper presents the usefulness of the circuit simulator Multisim software moreover, the understanding the operation of full-wave single phase rectifier circuit with RC load and study the effect of the load resistance  on the voltage, output current were discussed , the output power, the ripple factor and the total harmonic distortion ( THD).  Simulation results are existing along with the theoretical analysis. It can obtain a power factor by simulated is unity, to be excellent results
本文介绍了电路仿真软件Multisim的实用性,了解了RC负载下全波单相整流电路的工作原理,讨论了负载电阻对电压、输出电流、输出功率、纹波因数和总谐波失真(THD)的影响。仿真结果与理论分析相结合。通过仿真得到一个功率因数,得到了很好的效果
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引用次数: 0
Molecular''Detection of''Coxiella burnitii Among''Aborted Women in Thi-Qar Province/ Iraq Hekmat K. Ateya 伊拉克Thi-Qar省流产妇女中“伯氏杆菌”的分子检测
Pub Date : 2019-04-24 DOI: 10.32792/utq/utj/vol12/4/7
H. Ateya
Coxiella burnetii ''bacteria, an obligate''intracellular parasite with''a worldwide geographical distribution, are the causative''agent of Q fever in humans. In this study a total of 47 blood samples collected from aborted women in Bent Al Huda hospital in Thi- Qar province in southern Iraq. By a polymerase chain reactions (PCR) ''assay targeting''IS 1111 and'' com1 gene s of this bacterium.The C . burnetii diagnosed in 8(17.02 % ) clinical samples and the frequent''association of the pathogen with cases of spontaneous abortion. This first study on this bacterium which remains not diagnosed as causative agents of abortion and its prevalence in Iraq is non-studied.
伯纳蒂克希菌是一种专性细胞内寄生虫,在世界范围内分布,是人类Q热的致病因子。本研究共采集了伊拉克南部Thi- Qar省Bent Al Huda医院流产妇女的47份血液样本。通过聚合酶链反应(PCR)“试验靶向”IS 1111和“com1基因”s。C。8例(17.02%)临床标本检出伯纳蒂菌,常与自然流产病例相关。对这种尚未被诊断为流产病原体的细菌及其在伊拉克的流行情况进行的首次研究没有进行研究。
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引用次数: 0
期刊
University of Thi-Qar Journal
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