Pub Date : 2024-04-17DOI: 10.25073/2588-1140/vnunst.5629
Tran Cao Son, Luu Thi Huyen Trang, Do Thi Thu Hang, Kieu Thi Lan Phuong, Nguyen Ngoc Son, Pham Thanh Liem
Tobacco is one of the biggest public health threats facing the world in general and Vietnam in particular. More than 8 million people around the world die from tobacco-related diseases. There are about 70 carcinogens in cigarettes, in addition to addictive, cytotoxic, mutagenic active ingredients,... Among them, Tar and Nicotin are the biggest dangers affecting people health in cigarette smoke. In the study, analysis of Tar and Nicotin content in 166 cigarette samples taken from 10 cigarette manufacturing companies in Vietnam was performed. Analytical results show that Tar content ranges from 1.67 to 15.1 mg/cigarette; Nicotin content ranges from 0.37 to 1.35 mg/cigarette. These results are all lower than the maximum allowable limit according to QCVN 16-1:2015/BYT, but there are some samples with Tar and Nicotin content close to the asymptotic level. This analysis result is also consistent with reports of Tobacco Institute Company Limited in recent years. Although the Tar and Nicotin contents in the samples do not exceed the maximum limit according to QCVN, they are much higher than other countries in the world. Therefore, it is necessary to propose appropriate routes to reduce Tar and Nicotin to ensure the health of users.
{"title":"Analysis and Evaluation of Tar and Nicotin Content in some Cigarette Products of Vietnam in 2023","authors":"Tran Cao Son, Luu Thi Huyen Trang, Do Thi Thu Hang, Kieu Thi Lan Phuong, Nguyen Ngoc Son, Pham Thanh Liem","doi":"10.25073/2588-1140/vnunst.5629","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5629","url":null,"abstract":"Tobacco is one of the biggest public health threats facing the world in general and Vietnam in particular. More than 8 million people around the world die from tobacco-related diseases. There are about 70 carcinogens in cigarettes, in addition to addictive, cytotoxic, mutagenic active ingredients,... Among them, Tar and Nicotin are the biggest dangers affecting people health in cigarette smoke. In the study, analysis of Tar and Nicotin content in 166 cigarette samples taken from 10 cigarette manufacturing companies in Vietnam was performed. Analytical results show that Tar content ranges from 1.67 to 15.1 mg/cigarette; Nicotin content ranges from 0.37 to 1.35 mg/cigarette. These results are all lower than the maximum allowable limit according to QCVN 16-1:2015/BYT, but there are some samples with Tar and Nicotin content close to the asymptotic level. This analysis result is also consistent with reports of Tobacco Institute Company Limited in recent years. Although the Tar and Nicotin contents in the samples do not exceed the maximum limit according to QCVN, they are much higher than other countries in the world. Therefore, it is necessary to propose appropriate routes to reduce Tar and Nicotin to ensure the health of users.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140693861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.25073/2588-1140/vnunst.5642
Dang Minh Huong Giang, Nguyen Duc Hieu, Chu Thị Huệ, Pham Thi Ngoc Mai, Nguyen Thi Anh Huong, Hoang Quoc Anh
Polycyclic aromatic hydrocarbons (PAHs) and their derivatives are a diverse group of organic pollutants, which are relatively persistent and toxic. Simultaneous determination of unsubstituted PAHs and methylated PAHs (Me-PAHs) is necessary to provide comprehensive insights into contamination levels and sources of these pollutants. In this study, we collected surface sediment samples in Vietnamese central coast to analyze 7 PAHs and 12 Me-PAHs. The sediment samples were extracted by using a focused ultrasonic processor with acetone/hexane (1:1) mixture and toluene. The extract was purified by column chromatography technique with activated silica gel and dichloromethane/hexane (1:3) as elution solvent. PAHs and Me-PAHs were determined by a gas chromatography/mass spectrometry (GC/MS) system operated in electron impact ionization (EI) and selected ion monitoring mode (SIM). Concentrations of total 7 PAHs and 12 Me-PAHs ranged from 30 to 246 (average 92) nanograms per gram sediment (ng/g). These levels were comparable to or lower than those measured in Vietnamese river sediments and marine sediments from some other countries in the world. The accumulation profiles of PAHs and Me-PAHs indicated that their emissions are likely associated with mixed pyrogenic and petrogenic sources. Our results have provided preliminary information about the pollution degree and accumulation characteristics of these substances in Vietnamese marine environments. Additional comprehensive studies should be performed to characterize contamination characterisrics, emission sources, and ecological risks of PAHs and their derivatives in this country.
{"title":"Analysis and Assessment of Contamination Degree and Emission Sources of Polycyclic Aromatic Hydrocarbons (PAHs and Me-PAHs) in Surface Sediments from Central Coast of Vietnam","authors":"Dang Minh Huong Giang, Nguyen Duc Hieu, Chu Thị Huệ, Pham Thi Ngoc Mai, Nguyen Thi Anh Huong, Hoang Quoc Anh","doi":"10.25073/2588-1140/vnunst.5642","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5642","url":null,"abstract":"Polycyclic aromatic hydrocarbons (PAHs) and their derivatives are a diverse group of organic pollutants, which are relatively persistent and toxic. Simultaneous determination of unsubstituted PAHs and methylated PAHs (Me-PAHs) is necessary to provide comprehensive insights into contamination levels and sources of these pollutants. In this study, we collected surface sediment samples in Vietnamese central coast to analyze 7 PAHs and 12 Me-PAHs. The sediment samples were extracted by using a focused ultrasonic processor with acetone/hexane (1:1) mixture and toluene. The extract was purified by column chromatography technique with activated silica gel and dichloromethane/hexane (1:3) as elution solvent. PAHs and Me-PAHs were determined by a gas chromatography/mass spectrometry (GC/MS) system operated in electron impact ionization (EI) and selected ion monitoring mode (SIM). Concentrations of total 7 PAHs and 12 Me-PAHs ranged from 30 to 246 (average 92) nanograms per gram sediment (ng/g). These levels were comparable to or lower than those measured in Vietnamese river sediments and marine sediments from some other countries in the world. The accumulation profiles of PAHs and Me-PAHs indicated that their emissions are likely associated with mixed pyrogenic and petrogenic sources. Our results have provided preliminary information about the pollution degree and accumulation characteristics of these substances in Vietnamese marine environments. Additional comprehensive studies should be performed to characterize contamination characterisrics, emission sources, and ecological risks of PAHs and their derivatives in this country.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140727578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.25073/2588-1140/vnunst.5624
Vu Van Sang, Nicholas Andronicos
The study was carried out to clone sheep cytokine genes for protein expression in mammalian cell culture. Once expressed it is hypothesized that these proteins when added to a primary mast cell culture will allow the growth of these cells. Mast cells are used by sheep to combat gastro-intestinal nematode (GIN) by helping to establish the host immune response and to eliminate of GIN. Kit ligand and IL3 proteins are growth factors of mast cells that were derived from extraction of total RNA from lung and thymus tissues, respectively. The IL3 and kit ligand genes were cloned into bacterial expression pGEM – T easy vector to determine correct sequences before being sub-cloned into the mammalian expression pWPI vector. The Pac 1 enzyme was used to cut restriction sites of pGEM – T easy vector, pWPI vector, IL3 and kit ligand genes in cloning strategies. The study also established 24 different conditions for transient transfection IL3 and Kit ligand genes into HT29 cells and screen positive cells on the flow cytometry. Results showed that clones of IL3 and kit ligand with the correct sequences were created for using transient transfection. The transfection efficiency of IL3 and kit ligand DNA/ pWPI plasmid into HT29 cells were extremely low with approximately 9% for the best condition. This low transfection efficiency leads to shortage of IL3 and kit ligand for growing mast cells. Therefore, stable transfection will be required to have highest efficiencies that produce the large quantities of IL3 and kit ligand proteins required for culture of sheep mast cell lines.
{"title":"Cloning Sheep Cytokin Genes and Preliminary Transfect to HT29 Cell Line","authors":"Vu Van Sang, Nicholas Andronicos","doi":"10.25073/2588-1140/vnunst.5624","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5624","url":null,"abstract":"The study was carried out to clone sheep cytokine genes for protein expression in mammalian cell culture. Once expressed it is hypothesized that these proteins when added to a primary mast cell culture will allow the growth of these cells. Mast cells are used by sheep to combat gastro-intestinal nematode (GIN) by helping to establish the host immune response and to eliminate of GIN. Kit ligand and IL3 proteins are growth factors of mast cells that were derived from extraction of total RNA from lung and thymus tissues, respectively. The IL3 and kit ligand genes were cloned into bacterial expression pGEM – T easy vector to determine correct sequences before being sub-cloned into the mammalian expression pWPI vector. The Pac 1 enzyme was used to cut restriction sites of pGEM – T easy vector, pWPI vector, IL3 and kit ligand genes in cloning strategies. The study also established 24 different conditions for transient transfection IL3 and Kit ligand genes into HT29 cells and screen positive cells on the flow cytometry. Results showed that clones of IL3 and kit ligand with the correct sequences were created for using transient transfection. The transfection efficiency of IL3 and kit ligand DNA/ pWPI plasmid into HT29 cells were extremely low with approximately 9% for the best condition. This low transfection efficiency leads to shortage of IL3 and kit ligand for growing mast cells. Therefore, stable transfection will be required to have highest efficiencies that produce the large quantities of IL3 and kit ligand proteins required for culture of sheep mast cell lines.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140727472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the study, NiFe2O4 was successfully synthesized by hydrothermal method for treatment of residual tetracycline (TC) in aqueous environment. The study also investigated effects of H2O2 as electron an acceptor to enhance TC photocatalytic degradation of the synthesized NiFe2O4. The properties of synthesized materials were determined by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-Vis absoprtion spectroscopy (UV-VIS) and vibrating sample magnetometer (VSM) systems. The obtained results indicated that the synthesized NiFe2O4 were nano-particles with average size of approximately 50 nm. The synthesized NiFe2O4 also exhibited high visible light absorption and magnetic ability. The TC removal results indicated that the NiFe2O4 adsorbed certain amount of tetracycline under dark condition. Under visible light, the NiFe2O4 further degraded significant tetracycline amount. Finally, the study investigated that H2O2 effectively acted as electron acceptor for hydroxyl radical production to degrade tetracycline.
{"title":"Synthesis of NiFe2O4 Photocatalyst to Apply for Treatment of Residual Tetracycline in Aqueous Environment with Addition of H2O2","authors":"Dang Minh Duc, Cat Minh Hang, Dinh Tuong Van, Nguyen Viet Khoa, Tran Thuy Linh, Nguyen Thi Hanh, Pham Thanh Dong","doi":"10.25073/2588-1140/vnunst.5654","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5654","url":null,"abstract":"In the study, NiFe2O4 was successfully synthesized by hydrothermal method for treatment of residual tetracycline (TC) in aqueous environment. The study also investigated effects of H2O2 as electron an acceptor to enhance TC photocatalytic degradation of the synthesized NiFe2O4. The properties of synthesized materials were determined by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-Vis absoprtion spectroscopy (UV-VIS) and vibrating sample magnetometer (VSM) systems. The obtained results indicated that the synthesized NiFe2O4 were nano-particles with average size of approximately 50 nm. The synthesized NiFe2O4 also exhibited high visible light absorption and magnetic ability. The TC removal results indicated that the NiFe2O4 adsorbed certain amount of tetracycline under dark condition. Under visible light, the NiFe2O4 further degraded significant tetracycline amount. Finally, the study investigated that H2O2 effectively acted as electron acceptor for hydroxyl radical production to degrade tetracycline.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140725545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.25073/2588-1140/vnunst.5605
T. Cong, Nguyen Linh Chi, Nguyen Ha Thanh, D. T. T. Anh, Ban Van Phuc, Hoang Mai Ha, Tran Quang Vinh, Le Nhat Thuy Giang, Nguyen Thi Quynh Giang, Nguyen Van Tuyen
Four flame retardant DOPO derivatives were succesfully synthesized by Pudovik reaction, including 6,6'-(([1,1'-biphenyl]-4,4'-diylbis(azanediyl))bis (thiophen-2-ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5a, 6,6'-(((sulfonylbis(4,1-phenylene))bis(azanediyl)) bis(thiophen-2-ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5b, 6,6'-((1,4-phenylenebis(azanediyl))bis(furan-2ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5c and 6,6'-((oxybis(4,1-phenylene))bis(1-(thiophen-2-yl)ethane-2,1-diyl))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5d. Among them, flame retardants 5a and 5d were synthesized for the first time. DOPO derivatives were combined with functionalized ammonium polyphosphate using polyethyleneimine (PEI-APP) as flame retardant systems for epoxy composites. The combination of compound 5a and PEI-APP gave the materials with the best flame retardancy. 6wt% of PEI-APP/6% of 5a promoted the composite material to V-0 rating in UL-94 vertical burning test and the limiting oxygen index reached 28.9%. The impact of flame retardants towards the mechanical and physical properties of epoxy resin was also evaluated. The combination of 5a with PEI-APP showed the least decrease in mechanical strength of epoxy composite.
{"title":"Synthesis of DOPO Derivatives for the Fabrication of Flame Retardant Epoxy Composites","authors":"T. Cong, Nguyen Linh Chi, Nguyen Ha Thanh, D. T. T. Anh, Ban Van Phuc, Hoang Mai Ha, Tran Quang Vinh, Le Nhat Thuy Giang, Nguyen Thi Quynh Giang, Nguyen Van Tuyen","doi":"10.25073/2588-1140/vnunst.5605","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5605","url":null,"abstract":"Four flame retardant DOPO derivatives were succesfully synthesized by Pudovik reaction, including 6,6'-(([1,1'-biphenyl]-4,4'-diylbis(azanediyl))bis (thiophen-2-ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5a, 6,6'-(((sulfonylbis(4,1-phenylene))bis(azanediyl)) bis(thiophen-2-ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5b, 6,6'-((1,4-phenylenebis(azanediyl))bis(furan-2ylmethylene))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5c and 6,6'-((oxybis(4,1-phenylene))bis(1-(thiophen-2-yl)ethane-2,1-diyl))bis(dibenzo[c,e][1,2]oxaphosphinine 6-oxide) 5d. Among them, flame retardants 5a and 5d were synthesized for the first time. DOPO derivatives were combined with functionalized ammonium polyphosphate using polyethyleneimine (PEI-APP) as flame retardant systems for epoxy composites. The combination of compound 5a and PEI-APP gave the materials with the best flame retardancy. 6wt% of PEI-APP/6% of 5a promoted the composite material to V-0 rating in UL-94 vertical burning test and the limiting oxygen index reached 28.9%. The impact of flame retardants towards the mechanical and physical properties of epoxy resin was also evaluated. The combination of 5a with PEI-APP showed the least decrease in mechanical strength of epoxy composite.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.25073/2588-1140/vnunst.5604
Nguyen Vu Ngoc Mai, Nguyen Thi Ly Na, Tran Thi Thu Phuong, Do Minh The, Nguyen Thi Thanh Binh, Vo Manh Tien, Nguyễn Trí Quốc, Nguyen Thi Kim Chi, Mai Hung Thanh Tung, Cao Van Hoang
In this paper, La2O3/SiO2 material was synthesized and applied for growth stimulants of Salvia miltiorrhiza Bunge. La2O3/SiO2 material was successfully synthesized from La(NO3)3 and SiO2 precursors by solid-phase thermal method. The material properties were confirmed through physicochemical characterization methods including XRD, EDX, IR, and SEM analysis. The results of using La2O3/SiO2 as a stimulating plant growth material for Salvia miltiorrhiza plants indicated that the use of La2O3/SiO2 on Salvia miltiorrhiza plants had a significant effect. In the experimental model, the dry root yield increased by 0.27 tons/hecta, the root length increased by 3.6 cm on average, and the harvest time for Salvia miltiorrhiza was eleven days earlier. Additionally, the Salvia miltiorrhiza with La2O3/SiO2 material was tougher than that without it, and gold leaf symptom was not observed in the experimental Salvia miltiorrhiza. Thus, the data suggested that La2O3/SiO2 had a favorable effect on alvia miltiorrhiza the harvest time for Salvia miltiorrhiza was eleven days.
{"title":"Synthesis of Materials Based on La2O3 and SiO2 as Growth Stimulants for Salvia miltiorrhiza Bunge","authors":"Nguyen Vu Ngoc Mai, Nguyen Thi Ly Na, Tran Thi Thu Phuong, Do Minh The, Nguyen Thi Thanh Binh, Vo Manh Tien, Nguyễn Trí Quốc, Nguyen Thi Kim Chi, Mai Hung Thanh Tung, Cao Van Hoang","doi":"10.25073/2588-1140/vnunst.5604","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5604","url":null,"abstract":"In this paper, La2O3/SiO2 material was synthesized and applied for growth stimulants of Salvia miltiorrhiza Bunge. La2O3/SiO2 material was successfully synthesized from La(NO3)3 and SiO2 precursors by solid-phase thermal method. The material properties were confirmed through physicochemical characterization methods including XRD, EDX, IR, and SEM analysis. The results of using La2O3/SiO2 as a stimulating plant growth material for Salvia miltiorrhiza plants indicated that the use of La2O3/SiO2 on Salvia miltiorrhiza plants had a significant effect. In the experimental model, the dry root yield increased by 0.27 tons/hecta, the root length increased by 3.6 cm on average, and the harvest time for Salvia miltiorrhiza was eleven days earlier. Additionally, the Salvia miltiorrhiza with La2O3/SiO2 material was tougher than that without it, and gold leaf symptom was not observed in the experimental Salvia miltiorrhiza. Thus, the data suggested that La2O3/SiO2 had a favorable effect on alvia miltiorrhiza the harvest time for Salvia miltiorrhiza was eleven days.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-10DOI: 10.25073/2588-1140/vnunst.5487
Nguyen Thi Tu Linh, Vu Thu Huong, Le Thi Thanh Nhan, Lê Lan Phương, Nguyen Doan Thuy, Nguyen Van Lieu, Nguyen Thi Van Anh, T. Thái
Mitochondrial dysfunction, caused by large-scale deletion mutations, can lead to impaired function of the mitochondrial respiratory chain, reduced ATP production, and serious effects on most energy-consuming organs, such as neurons, and can induce seizures in epilepsy. Carbamazepine (CBZ), the first-line drug used in the treatment of epilepsy, can be harmful to mitochondria and its side effects may be related to mitochondrial dysfunction. In this study, mitochondrial DNA (mtDNA) large-scale deletions were identified in 65 CBZ-treated patients with epilepsy, including 32 patients with CBZ-induced hypersensitivity and 33 with CBZ tolerance. Using the PCR method, mtDNA large-scale deletions were identified in 21/65 epilepsy patients (32.31%), including 9/32 CBZ-hypersensitivity patients (28.13%) and 12/33 CBZ-tolerance patients (36.36%). However, this difference was not statistically significant. The “common deletion” of 4977 bp was the most prevalent deletion. Remarkably, the new deletion of 4876 bp in a CBZ-tolerance patient was reported for the first time. Quantitative PCR analysis showed that the level of mtDNA large-scale deletion was significantly lower in the hypersensitivity group than in the tolerance group (p < 0.05). Besides, analysis of the association between the level of mtDNA large-scale deletion and mtDNA copy number with the clinical features of CBZ hypersensitivity patients showed no relationship with age and severity of skin lesions (p > 0.05). However, there was a statistically significant association between the level of mtDNA large-scale deletions and sex (p < 0.01). Further studies are needed to evaluate the role of mtDNA large-scale deletions in epilepsy and their association with antiepileptic drugs.
{"title":"Large-Scale Deletions of Mitochondrial DNA in Epilepsy Patients Treated with Carbamazepine","authors":"Nguyen Thi Tu Linh, Vu Thu Huong, Le Thi Thanh Nhan, Lê Lan Phương, Nguyen Doan Thuy, Nguyen Van Lieu, Nguyen Thi Van Anh, T. Thái","doi":"10.25073/2588-1140/vnunst.5487","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5487","url":null,"abstract":"Mitochondrial dysfunction, caused by large-scale deletion mutations, can lead to impaired function of the mitochondrial respiratory chain, reduced ATP production, and serious effects on most energy-consuming organs, such as neurons, and can induce seizures in epilepsy. Carbamazepine (CBZ), the first-line drug used in the treatment of epilepsy, can be harmful to mitochondria and its side effects may be related to mitochondrial dysfunction. In this study, mitochondrial DNA (mtDNA) large-scale deletions were identified in 65 CBZ-treated patients with epilepsy, including 32 patients with CBZ-induced hypersensitivity and 33 with CBZ tolerance. Using the PCR method, mtDNA large-scale deletions were identified in 21/65 epilepsy patients (32.31%), including 9/32 CBZ-hypersensitivity patients (28.13%) and 12/33 CBZ-tolerance patients (36.36%). However, this difference was not statistically significant. The “common deletion” of 4977 bp was the most prevalent deletion. Remarkably, the new deletion of 4876 bp in a CBZ-tolerance patient was reported for the first time. Quantitative PCR analysis showed that the level of mtDNA large-scale deletion was significantly lower in the hypersensitivity group than in the tolerance group (p < 0.05). Besides, analysis of the association between the level of mtDNA large-scale deletion and mtDNA copy number with the clinical features of CBZ hypersensitivity patients showed no relationship with age and severity of skin lesions (p > 0.05). However, there was a statistically significant association between the level of mtDNA large-scale deletions and sex (p < 0.01). Further studies are needed to evaluate the role of mtDNA large-scale deletions in epilepsy and their association with antiepileptic drugs.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-10DOI: 10.25073/2588-1140/vnunst.5404
Nguyen Cuong Quoc, Le Dang Quang, Nguyen Duy Tuan, Trần Duy Khang, Nguyen Vu Linh, Tran Thanh Men, Nguyen Trong Tuan, B. Hue, Tran Quang De
Based on the strong activity of belinostat, the study synthesized of some cinnamoylhydroxamic acid derivatives with the aim of creating new compounds that have the potential to selectively inhibit HDAC to contribute to cancer treatment. N-hydroxycinnamamide serves as both ZBG and the linker group. The derivatives orient to the HDAC2 and HDAC8 enzymes by molecular docking. Compound 9a (bearing sulfonamide) exhibited as the most potential candidate to inhibit the function of HDAC2 enzyme.
{"title":"Synthesis of Cinnamoylhydroxamic Acid Derivatives Bearing Sulfonamide, Amide Bonds as Connecting Unit Targeting Histone Deacetylases","authors":"Nguyen Cuong Quoc, Le Dang Quang, Nguyen Duy Tuan, Trần Duy Khang, Nguyen Vu Linh, Tran Thanh Men, Nguyen Trong Tuan, B. Hue, Tran Quang De","doi":"10.25073/2588-1140/vnunst.5404","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5404","url":null,"abstract":"Based on the strong activity of belinostat, the study synthesized of some cinnamoylhydroxamic acid derivatives with the aim of creating new compounds that have the potential to selectively inhibit HDAC to contribute to cancer treatment. N-hydroxycinnamamide serves as both ZBG and the linker group. The derivatives orient to the HDAC2 and HDAC8 enzymes by molecular docking. Compound 9a (bearing sulfonamide) exhibited as the most potential candidate to inhibit the function of HDAC2 enzyme.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139441182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.25073/2588-1140/vnunst.5583
Nguyen Thi Ngoc, Hoang Hai Duc
Congenital hand deformities are a group of birth defects related to abnormal development of soft, cartilage, or bone tissues in the hands. Recent studies have indicated that the most usual reason of congenital hand anomalies are due to the genetic factors. In this study, we sequenced the exome of a 2.5-year-old female patient case with bilateral hand deformity. Results revealed that the patient did not carry mutations in the expression region of the GLI3 and SHH genes. However, the patient did have a heterozygous mutation: FGFR2: c.C758G, p.P253R. Sanger sequencing confirmed this was a de novo mutation as it was not detected in the parents. In silico analyses indicated that P253R has a potential effect to alter the structure and function of the fibroblast growth factor receptor, consequently disrupting the processes involved in the formation and development of bone and cartilage tissues. Overall, this research will contribute to a better understanding of the role and functionality of the FGF molecular signaling network in the development and synthesis of bone and cartilage tissues, particularly during the embryonic stage.
{"title":"Detection of the de novo Pro253Arg Mutation of Fibroblast Growth Factor Receptor 2 (FGFR2) in a Pediatric Patient with Congenital Hand Anomaly","authors":"Nguyen Thi Ngoc, Hoang Hai Duc","doi":"10.25073/2588-1140/vnunst.5583","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5583","url":null,"abstract":"Congenital hand deformities are a group of birth defects related to abnormal development of soft, cartilage, or bone tissues in the hands. Recent studies have indicated that the most usual reason of congenital hand anomalies are due to the genetic factors. In this study, we sequenced the exome of a 2.5-year-old female patient case with bilateral hand deformity. Results revealed that the patient did not carry mutations in the expression region of the GLI3 and SHH genes. However, the patient did have a heterozygous mutation: FGFR2: c.C758G, p.P253R. Sanger sequencing confirmed this was a de novo mutation as it was not detected in the parents. In silico analyses indicated that P253R has a potential effect to alter the structure and function of the fibroblast growth factor receptor, consequently disrupting the processes involved in the formation and development of bone and cartilage tissues. Overall, this research will contribute to a better understanding of the role and functionality of the FGF molecular signaling network in the development and synthesis of bone and cartilage tissues, particularly during the embryonic stage.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139198870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.25073/2588-1140/vnunst.5614
Vu Van Tu, Nguyen Viet Anh, Vu Thi Thanh Nhai, Nguyen Thi Kim Oanh, T. Minh, T. Tri
In this study, ten phthalic acid esters (PAEs) were measured in indoor and outdoor air samples collected from Hanoi metropolitan areas. The target compounds were adsorbed onto a solid adsorbent matrix (C18) with a low-speed pump and then analyzed on a gas chromatography tandem mass spectrometry. The recoveries of surrogate standards ranged from 78.5 to 112% (RSD < 15%). The method detection limits of target compounds were in the range of 0.08-0.3 ng/m3. The total mean concentration of 10 PAEs in indoor and outdoor samples were in ranges of 1400-3710 ng/m3 and 178-487 ng/m3, respectively. Among PAEs, di-(2-ethyl)hexyl phthalate (DEHP) was found at the highest abundance and concentration. The distribution of PAEs in indoor air was higher significantly than those in outdoor air samples. Some pairs of PAEs (BzBP vs DCHP, DEHP vs DnHP, DnHP vs DnOP, and DEHP vs DnOP) had strong correlations and suggested that they are from the same source. Human exposure doses to PAEs through inhalation were estimated for two age groups (children and adults) based on the measured concentrations of PAEs in air, inhalation rates, and body weights. The estimated daily intake doses were in ranges of 29.7-750 ng/kg-bw/d and 7.29-181 ng/kg-bw/d for children and adults, respectively.
本研究测量了河内市区采集的室内和室外空气样本中的十种邻苯二甲酸酯（PAEs）。使用低速泵将目标化合物吸附到固体吸附剂基质（C18）上，然后用气相色谱串联质谱仪进行分析。代用标准品的回收率为 78.5%至 112%（RSD < 15%）。目标化合物的方法检出限为 0.08-0.3 纳克/立方米。室内和室外样本中 10 种 PAE 的总平均浓度分别为 1400 至 3710 纳克/立方米和 178 至 487 纳克/立方米。在 PAEs 中，以邻苯二甲酸二（2-乙基）己酯（DEHP）的含量和浓度最高。室内空气中 PAEs 的分布明显高于室外空气样本。一些 PAEs 对（BzBP 与 DCHP、DEHP 与 DnHP、DnHP 与 DnOP 和 DEHP 与 DnOP）具有很强的相关性，表明它们来自同一来源。根据测得的空气中 PAEs 浓度、吸入率和体重，估算了两个年龄组（儿童和成人）人类通过吸入接触 PAEs 的剂量。儿童和成人的估计日摄入剂量范围分别为 29.7-750 纳克/千克-体重/天和 7.29-181 纳克/千克-体重/天。
{"title":"Distribution of Phthalate Acid Esters in Indoor and Outdoor Air Samples Collected from Hanoi Metropolitan Areas in Vietnam","authors":"Vu Van Tu, Nguyen Viet Anh, Vu Thi Thanh Nhai, Nguyen Thi Kim Oanh, T. Minh, T. Tri","doi":"10.25073/2588-1140/vnunst.5614","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5614","url":null,"abstract":"In this study, ten phthalic acid esters (PAEs) were measured in indoor and outdoor air samples collected from Hanoi metropolitan areas. The target compounds were adsorbed onto a solid adsorbent matrix (C18) with a low-speed pump and then analyzed on a gas chromatography tandem mass spectrometry. The recoveries of surrogate standards ranged from 78.5 to 112% (RSD < 15%). The method detection limits of target compounds were in the range of 0.08-0.3 ng/m3. The total mean concentration of 10 PAEs in indoor and outdoor samples were in ranges of 1400-3710 ng/m3 and 178-487 ng/m3, respectively. Among PAEs, di-(2-ethyl)hexyl phthalate (DEHP) was found at the highest abundance and concentration. The distribution of PAEs in indoor air was higher significantly than those in outdoor air samples. Some pairs of PAEs (BzBP vs DCHP, DEHP vs DnHP, DnHP vs DnOP, and DEHP vs DnOP) had strong correlations and suggested that they are from the same source. Human exposure doses to PAEs through inhalation were estimated for two age groups (children and adults) based on the measured concentrations of PAEs in air, inhalation rates, and body weights. The estimated daily intake doses were in ranges of 29.7-750 ng/kg-bw/d and 7.29-181 ng/kg-bw/d for children and adults, respectively.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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