Pub Date : 2022-06-23DOI: 10.25073/2588-1140/vnunst.5353
Le Nhat Thuy Giang, D. T. T. Anh, Nguyen Ha Thanh, H. T. Phuong, Nguyen Tuan Anh, Nguyen Thi Quynh Giang, Vu Thi Thai Ha, N. Tuyen
The green and efficient approach for synthesis of 2-hydroxy-3-(arylmethyl)(4-hydroxy-2-oxo-2,5-dihydrofuran-3-yl)-1,4-naphthoquinones starting from 2-hydroxy-1,4-naphthoquinone, tetronic acid, and aromatic aldehydes was developed. These reactions were carried out in ethanol under microwave irradiation in the presence of ammonium acetate as catalyst. These multicomponent domino reactions presumably occur via NH4OAc-catalyzed Mannich reaction - Michael addition - hydration - tautomerism - elimination sequence of reactions.
{"title":"A Green and Efficient NH4OAc-catalyzed Synthesis of 2-Hydroxy-3-(arylmethyl)(4-hydroxy-2-oxo-2,5-dihydrofuran-3-yl)-1,4-naphthoquinones","authors":"Le Nhat Thuy Giang, D. T. T. Anh, Nguyen Ha Thanh, H. T. Phuong, Nguyen Tuan Anh, Nguyen Thi Quynh Giang, Vu Thi Thai Ha, N. Tuyen","doi":"10.25073/2588-1140/vnunst.5353","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5353","url":null,"abstract":"The green and efficient approach for synthesis of 2-hydroxy-3-(arylmethyl)(4-hydroxy-2-oxo-2,5-dihydrofuran-3-yl)-1,4-naphthoquinones starting from 2-hydroxy-1,4-naphthoquinone, tetronic acid, and aromatic aldehydes was developed. These reactions were carried out in ethanol under microwave irradiation in the presence of ammonium acetate as catalyst. These multicomponent domino reactions presumably occur via NH4OAc-catalyzed Mannich reaction - Michael addition - hydration - tautomerism - elimination sequence of reactions.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77142447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.25073/2588-1140/vnunst.5264
P. Phu, Pham Thi Thao Nhi, Hoàng Vũ Trụ, Cao Thi Phi Nga
A list of 23 species belonging to 15 genera of subfamily Cetoniinae is reported from Phia Oac-Phia Den National Park, Cao Bang Province. Of which, eight genera and eleven species are recorded for the first time from this National Park. In addition, the global and domestic distributions of all recognized species are given.
{"title":"Species composition of the subfamily Cetoniinae (Coleoptera: Scarabaeidae) from Phia Oac-Phia Den National Park, Cao Bang Province","authors":"P. Phu, Pham Thi Thao Nhi, Hoàng Vũ Trụ, Cao Thi Phi Nga","doi":"10.25073/2588-1140/vnunst.5264","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5264","url":null,"abstract":"A list of 23 species belonging to 15 genera of subfamily Cetoniinae is reported from Phia Oac-Phia Den National Park, Cao Bang Province. Of which, eight genera and eleven species are recorded for the first time from this National Park. In addition, the global and domestic distributions of all recognized species are given.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91473119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.25073/2588-1140/vnunst.5334
N. Nhung, D. T. Kien, N. Hiep, N. Hanh
Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume. Therefore, cryopreservation of human umbilical cord (HUC) tissue has become an alternative solution to reduce the risks of disease and open up opportunities for later treatment for families in recent years. In this study, we evaluated the ability to isolate and the characteristic of mesenchymal stem cells (MSCs) derived from fresh and frozen human umbilical cord tissue. The results showed that the HUCMSCs were isolated successfully from both fresh and frozen HUC tissues. The expression of MSCs cell surface marker CD73, CD90, CD105 in group 1 were higher than those in group 2, CD73 (99,42 vs 95.2%); CD90 (99.74 vs 96.6%); CD105 (99.64 vs 96.15%), respectively. However, they are similar in morphology and osteogenic and adipose differentiated ability. The results of the study would be useful for stem cell research, cell therapy and regenerative medicine.
脐带间充质干细胞已被证实在细胞治疗和再生医学中是有效的。细胞低温保存被广泛用于长期保存。然而，细胞冷冻保存成本高且耗费人力。因此，近年来，人类脐带(HUC)组织的冷冻保存已成为降低疾病风险的替代解决方案，并为家庭提供了后期治疗的机会。在这项研究中，我们评估了从新鲜和冷冻的人脐带组织中分离间充质干细胞(MSCs)的能力和特性。结果表明，从新鲜和冷冻的HUC组织中均可成功分离到HUC间充质干细胞。MSCs表面标志物CD73、CD90、CD105在1组的表达高于2组(99.2%、42% vs 95.2%);CD90 (99.74 vs 96.6%);CD105 (99.64 vs 96.15%)。然而，它们在形态和成骨和脂肪分化能力上是相似的。该研究结果对干细胞研究、细胞治疗和再生医学具有重要意义。
{"title":"Evaluation the Ability to Isolate and Culture Stem Cells from Frozen Human Umbilical Cord Tissue","authors":"N. Nhung, D. T. Kien, N. Hiep, N. Hanh","doi":"10.25073/2588-1140/vnunst.5334","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5334","url":null,"abstract":"Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume. Therefore, cryopreservation of human umbilical cord (HUC) tissue has become an alternative solution to reduce the risks of disease and open up opportunities for later treatment for families in recent years. In this study, we evaluated the ability to isolate and the characteristic of mesenchymal stem cells (MSCs) derived from fresh and frozen human umbilical cord tissue. The results showed that the HUCMSCs were isolated successfully from both fresh and frozen HUC tissues. The expression of MSCs cell surface marker CD73, CD90, CD105 in group 1 were higher than those in group 2, CD73 (99,42 vs 95.2%); CD90 (99.74 vs 96.6%); CD105 (99.64 vs 96.15%), respectively. However, they are similar in morphology and osteogenic and adipose differentiated ability. The results of the study would be useful for stem cell research, cell therapy and regenerative medicine.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91273233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-18DOI: 10.25073/2588-1140/vnunst.5215
Tạ Thị Thu Thuỷ, D. T. Huong, Tran Duc Hau
Van Long Wetland Nature Reserve is the 9th Ramsar of Vietnam, which has high biodiversity. To assess water quality in this area, the present study initially used the Shannon-Wiener Diversity Index (H') and IBI (Index of Biotic Integrity) of fish community collected in two field surveys in September 2019 and May 2020. A total of 33 fish species belonging to 18 families, 8 orders were determined. The H' index was 1.983 and IBI as 32 indicated that the water quality in the Van Long Wetland Nature Reserve was poor, probably influencing on aquatic organisms. In general, physical-chemical analysis methods revealed that most of the water parameters in the surface layer are good, but many parameters ranged from B1 to A2 levels, especially for TP (Phosphate calculated as P) being at poor level of pollution (B1-B2). Thus, results obtained from both bio-indices and physical-chemical analysis seem similar and indicate that the water in Van Long Wetland Nature Reserve was not completely clean. This finding will contribute to providing data for the conservation of biodiversity and ecotourism development in the reserved area.
{"title":"Initial Usage of Fish Community to Assess Water Quality in Van Long Wetland Nature Reserve, Ninh Binh Province","authors":"Tạ Thị Thu Thuỷ, D. T. Huong, Tran Duc Hau","doi":"10.25073/2588-1140/vnunst.5215","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5215","url":null,"abstract":"Van Long Wetland Nature Reserve is the 9th Ramsar of Vietnam, which has high biodiversity. To assess water quality in this area, the present study initially used the Shannon-Wiener Diversity Index (H') and IBI (Index of Biotic Integrity) of fish community collected in two field surveys in September 2019 and May 2020. A total of 33 fish species belonging to 18 families, 8 orders were determined. The H' index was 1.983 and IBI as 32 indicated that the water quality in the Van Long Wetland Nature Reserve was poor, probably influencing on aquatic organisms. In general, physical-chemical analysis methods revealed that most of the water parameters in the surface layer are good, but many parameters ranged from B1 to A2 levels, especially for TP (Phosphate calculated as P) being at poor level of pollution (B1-B2). Thus, results obtained from both bio-indices and physical-chemical analysis seem similar and indicate that the water in Van Long Wetland Nature Reserve was not completely clean. This finding will contribute to providing data for the conservation of biodiversity and ecotourism development in the reserved area.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80823496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-15DOI: 10.25073/2588-1140/vnunst.5322
Thu Thuy Nguyen Thi
This study analyzed the chemical speciation of Pb metal in road dust samples from six provinces in northern Vietnam. Samples were extracted according to Tesser's sequential extraction procedure. [22] and measured by the ICP/MS method, with the total recovery of Pb ranged from 90.9% to 107%. The analysis results showed that Pb existed mainly in the form of residual fraction F5 (38.6%) > organic fraction (29.0%), > Fe/Mn oxide fraction (19.2%) > exchange fraction, and carbonate fraction (5.00% and 8.32%, respectively). The pollution indices such as geological accumulation index (Igeo), individual contamination factor (ICF), and risk assessment index (RAC%) were utilized to evaluate pollution levels. The values of Igeo of Pb in samples ranged from 1.80 to 2.65, with a mean value of 2.24. The value of ICF varied from 0.60 to 2.87, with a mean value of 2.04. The values of RAC ranged from 4.47% to 23.2%, with a mean value of 13.3%. In general, the concentration of Pb in most of the studied samples in the six provinces were classified as low pollution and risk levels according to Igeo, ICF and RAC. However, in some provinces which have many industries producing electronic components, steel ore, and densely populated urban areas, the pollution level of Pb was from moderate to large, potentially affecting the environment of the studied areas.
{"title":"Chemical Speciation, Risk Assessment and Pollution Level of Lead Metals in Road Dust of some Industry Zones and Urban Areas in Northern Vietnam","authors":"Thu Thuy Nguyen Thi","doi":"10.25073/2588-1140/vnunst.5322","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5322","url":null,"abstract":"This study analyzed the chemical speciation of Pb metal in road dust samples from six provinces in northern Vietnam. Samples were extracted according to Tesser's sequential extraction procedure. [22] and measured by the ICP/MS method, with the total recovery of Pb ranged from 90.9% to 107%. The analysis results showed that Pb existed mainly in the form of residual fraction F5 (38.6%) > organic fraction (29.0%), > Fe/Mn oxide fraction (19.2%) > exchange fraction, and carbonate fraction (5.00% and 8.32%, respectively). The pollution indices such as geological accumulation index (Igeo), individual contamination factor (ICF), and risk assessment index (RAC%) were utilized to evaluate pollution levels. The values of Igeo of Pb in samples ranged from 1.80 to 2.65, with a mean value of 2.24. The value of ICF varied from 0.60 to 2.87, with a mean value of 2.04. The values of RAC ranged from 4.47% to 23.2%, with a mean value of 13.3%. In general, the concentration of Pb in most of the studied samples in the six provinces were classified as low pollution and risk levels according to Igeo, ICF and RAC. However, in some provinces which have many industries producing electronic components, steel ore, and densely populated urban areas, the pollution level of Pb was from moderate to large, potentially affecting the environment of the studied areas.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80792724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-15DOI: 10.25073/2588-1140/vnunst.5171
Quang Dang Ngoc, H. Duong
Fractions yielded from Phellinus gilvus fruiting bodies by liquid-liquid partition of methanol extract contained various bioactive compounds such as phenolics, flavonoids and terpenes. Butanol, water and ethyl acetate fractions had high content of total phenolics, 158.12 – 247.31 mg of gallic acid equivalents (GAE)/g and low content of flavonoids, 21.74 - 36.05 mg of quercetin equivalents (QE)/g, respectively. These values highly correlated with their antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging potential with IC50 values of 84.5 -108.44 μg/mL and reducing power. These fractions showed moderate α-amylase inhibitory activity with IC50 values of 4.77, 10.69 and 3.9 mg/mL respectively. The P. gilvus fractions exhibited very slight inhibitory activity on the growth of Gram-positive and Gram-negative bacterial strains at the concentration of 20 mg/mL. The results suggest the utility of butanol, water, and ethyl acetate fractions from fruiting bodies of P. gilvus collected in Vietnam for further characterization in order to apply for diabetes or anti-aging prevention.
{"title":"Second metabolite content and antioxidative, α-amylase inhibitory activity of Phellinus gilvus fruiting body extracts","authors":"Quang Dang Ngoc, H. Duong","doi":"10.25073/2588-1140/vnunst.5171","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5171","url":null,"abstract":"Fractions yielded from Phellinus gilvus fruiting bodies by liquid-liquid partition of methanol extract contained various bioactive compounds such as phenolics, flavonoids and terpenes. Butanol, water and ethyl acetate fractions had high content of total phenolics, 158.12 – 247.31 mg of gallic acid equivalents (GAE)/g and low content of flavonoids, 21.74 - 36.05 mg of quercetin equivalents (QE)/g, respectively. These values highly correlated with their antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging potential with IC50 values of 84.5 -108.44 μg/mL and reducing power. These fractions showed moderate α-amylase inhibitory activity with IC50 values of 4.77, 10.69 and 3.9 mg/mL respectively. The P. gilvus fractions exhibited very slight inhibitory activity on the growth of Gram-positive and Gram-negative bacterial strains at the concentration of 20 mg/mL. The results suggest the utility of butanol, water, and ethyl acetate fractions from fruiting bodies of P. gilvus collected in Vietnam for further characterization in order to apply for diabetes or anti-aging prevention.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78326647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-15DOI: 10.25073/2588-1140/vnunst.5170
Trịnh Thị Thắm
Nghiên cứu này tập trung đánh giá mức độ phân bố hàm lượng của nhóm hóa chất bảo vệ thực vật họ clo hữu cơ (OCPs) và polyclhlorinated Biphenyls (PCBs) trong mẫu nước và trầm tích mặt tại sông Hồng đoạn chảy từ cuối địa phận thành phố Hà Nội đến thành phố Nam Định với chiều dài 85 km. Nhóm nghiên cứu đã tiến hành lấy 15 mẫu nước, 15 mẫu trầm tích mặt và tiến hành phân tích xác định hàm lượng OCPs, PCBs bằng sắc ký khí GC/ECD. Kết quả thu được hàm lượng tổng PCBs, HCHs, DDTs và tổng OCPs trong mẫu nước lần lượt dao động trong khoảng 0,013 ÷ 0, 033 µg/L; 0,601 ÷ 30,7 µg/L; 0,091 ÷ 2,73 µg/L và 1,27 ÷ 39,3 µg/L. Đồng thời, hàm lượng trung bình của các chất/nhóm chất trong trầm tích lần lượt là 23,3 µg/kg dw, 14,6 µg/kg dw, 1,16 µg/kg dw và 18,2 µg/kg dw. Kết quả đánh giá rủi ro bán định lượng đã chỉ ra mức độ rủi ro thấp đến trung bình của các chất/nhóm chất OCPs trong mẫu nước và mẫu trầm tích. Nghiên cứu là cơ sở khoa học để thực hiện các biện pháp kiểm soát ô nhiễm môi trường lưu vực sông
{"title":"Đánh giá hàm lượng và rủi ro sinh thái của một số hợp chất clo hữu cơ khó phân hủy trong nước và trầm tích mặt sông Hồng đoạn chảy từ Hà Nội đến Nam Định","authors":"Trịnh Thị Thắm","doi":"10.25073/2588-1140/vnunst.5170","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5170","url":null,"abstract":"Nghiên cứu này tập trung đánh giá mức độ phân bố hàm lượng của nhóm hóa chất bảo vệ thực vật họ clo hữu cơ (OCPs) và polyclhlorinated Biphenyls (PCBs) trong mẫu nước và trầm tích mặt tại sông Hồng đoạn chảy từ cuối địa phận thành phố Hà Nội đến thành phố Nam Định với chiều dài 85 km. Nhóm nghiên cứu đã tiến hành lấy 15 mẫu nước, 15 mẫu trầm tích mặt và tiến hành phân tích xác định hàm lượng OCPs, PCBs bằng sắc ký khí GC/ECD. Kết quả thu được hàm lượng tổng PCBs, HCHs, DDTs và tổng OCPs trong mẫu nước lần lượt dao động trong khoảng 0,013 ÷ 0, 033 µg/L; 0,601 ÷ 30,7 µg/L; 0,091 ÷ 2,73 µg/L và 1,27 ÷ 39,3 µg/L. Đồng thời, hàm lượng trung bình của các chất/nhóm chất trong trầm tích lần lượt là 23,3 µg/kg dw, 14,6 µg/kg dw, 1,16 µg/kg dw và 18,2 µg/kg dw. Kết quả đánh giá rủi ro bán định lượng đã chỉ ra mức độ rủi ro thấp đến trung bình của các chất/nhóm chất OCPs trong mẫu nước và mẫu trầm tích. Nghiên cứu là cơ sở khoa học để thực hiện các biện pháp kiểm soát ô nhiễm môi trường lưu vực sông","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86759545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-15DOI: 10.25073/2588-1140/vnunst.5174
Nguyễn Đình Thành, Nguyễn Minh Trí, Vũ Ngọc Toán, Hoàng Mai Linh, Ngô Thị Ngọc Mai, Trần Thị Hải Yến, Ngô Thị Thủy, Nguyễn Thị Thuý Hường, Phạm Thị Thúy Vân, Nguyễn Thị Kim Giang, Đặng Thị Tuyết Anh
Tóm tắt: Các dẫn xuất azide của isatin là các chất đầu cần thiết cho phản ứng click để tạo ra dị vòng 1,2,3-triazole. Các dẫn xuất N-(ω-bromoalkyl)isatin cần cho chuyển hoá được tổng hợp từ phản ứng thế nucleophile của các isatin tương ứng với các dibromoalkane thích hợp. Một số N-(ω-azidoalkyl)isatin có chứa nhóm thế hoặc không thế ở vòng benzene đã được tổng hợp bằng phản ứng của các N-(ω-bromoalkyl)isatin tương ứng đã tổng hợp được với natri azide. Phản ứng được thực hiện trong DMF khan với sự có mặt của KI với vai trò chất xúc tiến. Hiệu suất sản phẩm đạt được 30−85%. Abstract: Azide derivatives of isatins were the needed initial materials for click chemistry in order to form 1,2,3-triazoles. N-(ω-Bromoalkyl)isatins were prepared by nucleophilic reaction of (un)substituted isatins with appropriate dibromoalkanes. Some ω-azidoalkylisatins were synthesized by reaction of corresponding ω-bromoalkylisatins with sodium azide. The reactions were performed in dry DMF as solvent in the presence of KI as promotive agent. Product yields achived 30−85%.
总结:isatin的叠氮引物是触发点击反应产生1、2、3-三唑异常的关键物质。这些N -(ω衍生物-bromoalkyl)靛红需要给将被人工合成了从置换反应转换nucleophile这些对应的靛红和这些dibromoalkane恰当的。有些N -(ω-azidoalkyl)靛红含取代基或不环上取代的benzene已经是用你们的反应合成的N -(ω-bromoalkyl)对应的靛红能跟azide钠的方法合成过。在DMF khan中进行的反应是KI的存在作为催化剂。产品效率达到30−85%。摘要：异丙氨酸的叠氮衍生物是形成1,2,3-三唑所需的初始化学物质。N -(ω-Bromoalkyl) isatins在得克萨斯prepared by nucleophilic reaction of (un) substituted isatins access得到适当dibromoalkanes。各地打破ω-azidoalkylisatins在得克萨斯synthesized by reaction of correspondingω-bromoalkylisatins access sodium azide。在KI作为促进剂存在的情况下，以干DMF作为溶剂进行反应。Product yields achived 30−85%。
{"title":"Góp phần nghiên cứu tổng hợp một số N-(ω-azidoalkyl)isatin thế từ các isatin thế và không thế","authors":"Nguyễn Đình Thành, Nguyễn Minh Trí, Vũ Ngọc Toán, Hoàng Mai Linh, Ngô Thị Ngọc Mai, Trần Thị Hải Yến, Ngô Thị Thủy, Nguyễn Thị Thuý Hường, Phạm Thị Thúy Vân, Nguyễn Thị Kim Giang, Đặng Thị Tuyết Anh","doi":"10.25073/2588-1140/vnunst.5174","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5174","url":null,"abstract":"Tóm tắt: Các dẫn xuất azide của isatin là các chất đầu cần thiết cho phản ứng click để tạo ra dị vòng 1,2,3-triazole. Các dẫn xuất N-(ω-bromoalkyl)isatin cần cho chuyển hoá được tổng hợp từ phản ứng thế nucleophile của các isatin tương ứng với các dibromoalkane thích hợp. Một số N-(ω-azidoalkyl)isatin có chứa nhóm thế hoặc không thế ở vòng benzene đã được tổng hợp bằng phản ứng của các N-(ω-bromoalkyl)isatin tương ứng đã tổng hợp được với natri azide. Phản ứng được thực hiện trong DMF khan với sự có mặt của KI với vai trò chất xúc tiến. Hiệu suất sản phẩm đạt được 30−85%. \u0000Abstract: Azide derivatives of isatins were the needed initial materials for click chemistry in order to form 1,2,3-triazoles. N-(ω-Bromoalkyl)isatins were prepared by nucleophilic reaction of (un)substituted isatins with appropriate dibromoalkanes. Some ω-azidoalkylisatins were synthesized by reaction of corresponding ω-bromoalkylisatins with sodium azide. The reactions were performed in dry DMF as solvent in the presence of KI as promotive agent. Product yields achived 30−85%.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89368060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-12DOI: 10.25073/2588-1140/vnunst.5299
Bui Anh Thy, Nguyen Tang Truong, K. Phuc, N. T. Thu, N. V. Dũng, T. X. Hạnh, T. L. Thuoc
From the PRRSV virulent strain BG8 isolated from a PRRSV-infected pig, using serial passage method in MARC-145 cell line, we have successfully obtained an attenuated strain in 95th passage, named as BG895, with high potential to be a vaccine candidate. In this study, we present the results of the safety and efficacy evaluation of BG895 against PRRSV in experimental pigs. Trial results of vaccine formula using strain BG895 have very high safety when inoculating 5 doses/animal and 10 doses/animal. Evaluation of immune response by ELISA method showed that, from 14 days post inoculation, anti-PRRSV antibodies were detected in the serum of all inoculated pigs in vaccine batches with the lowest S/P index of 1.50 ± 0.4 and the highest S/P was 2.36 ± 0.1 from 28 days post inoculation. The IPMA method showed that the antibody titer of the vaccine reached ≥ 1/160 in 100% of pigs from 21 days post inoculation andreached ≥ 1/640 in 100% of pigs from 28 days post inoculation, indicating that the vaccine was effective at protecting 100% of pigs from 28 days post inoculation. The protective effect of the vaccine was evaluated by the virulent challenge from 28 days post inoculation with 1 dose/animal compared with the control group. The results showed that compared with all pigs in the control group with typical clinical manifestations of Blue-ear disease, all inoculated pigs had normal body temperature and weight gain, besides, the S/P index increased from 1.65 ± 0.1 to the highest 2.99 ± 0.2; the average antibody titer was >1/2560, and virus wasnot detected in nasal fluid by real-time RT-PCR from 7 days post challenge. These experimental results confirmed the safety and efficacy of the attenuated PRRS vaccine based on BG895strain.
从PRRSV感染猪中分离到的PRRSV毒力毒株BG8，采用MARC-145细胞系连续传代的方法，成功获得了第95代的减毒毒株，命名为BG895，具有很高的候选疫苗潜力。在本研究中，我们报告了BG895对实验猪PRRSV的安全性和有效性评价结果。试验结果表明，采用BG895菌株的疫苗配方在接种5剂/头和10剂/头时具有很高的安全性。ELISA法免疫应答评价结果显示，从接种后14 d开始，所有接种猪的血清中均检测到抗prrsv抗体，接种后28 d S/P指数最低为1.50±0.4，最高为2.36±0.1。IPMA法显示，接种后21天，100%的猪抗体效价达到≥1/160，接种后28天，100%的猪抗体效价达到≥1/640，表明该疫苗对接种后28天的猪有100%的保护作用。接种后28 d，每只动物接种1剂，用毒力攻毒法评价疫苗的保护效果。结果表明:与具有典型蓝耳病临床表现的对照组相比，接种猪的体温和体重增加均正常，S/P指数由1.65±0.1提高到最高的2.99±0.2;抗体平均滴度>1/2560，攻毒后7 d内鼻液中未检出病毒。这些实验结果证实了基于bg895株的PRRS减毒疫苗的安全性和有效性。
{"title":"The Safety and Efficacy Evaluation of PRRS Attenuated Vaccine BG895 Strain in Experimental Pigs","authors":"Bui Anh Thy, Nguyen Tang Truong, K. Phuc, N. T. Thu, N. V. Dũng, T. X. Hạnh, T. L. Thuoc","doi":"10.25073/2588-1140/vnunst.5299","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5299","url":null,"abstract":"From the PRRSV virulent strain BG8 isolated from a PRRSV-infected pig, using serial passage method in MARC-145 cell line, we have successfully obtained an attenuated strain in 95th passage, named as BG895, with high potential to be a vaccine candidate. In this study, we present the results of the safety and efficacy evaluation of BG895 against PRRSV in experimental pigs. Trial results of vaccine formula using strain BG895 have very high safety when inoculating 5 doses/animal and 10 doses/animal. Evaluation of immune response by ELISA method showed that, from 14 days post inoculation, anti-PRRSV antibodies were detected in the serum of all inoculated pigs in vaccine batches with the lowest S/P index of 1.50 ± 0.4 and the highest S/P was 2.36 ± 0.1 from 28 days post inoculation. The IPMA method showed that the antibody titer of the vaccine reached ≥ 1/160 in 100% of pigs from 21 days post inoculation andreached ≥ 1/640 in 100% of pigs from 28 days post inoculation, indicating that the vaccine was effective at protecting 100% of pigs from 28 days post inoculation. The protective effect of the vaccine was evaluated by the virulent challenge from 28 days post inoculation with 1 dose/animal compared with the control group. The results showed that compared with all pigs in the control group with typical clinical manifestations of Blue-ear disease, all inoculated pigs had normal body temperature and weight gain, besides, the S/P index increased from 1.65 ± 0.1 to the highest 2.99 ± 0.2; the average antibody titer was >1/2560, and virus wasnot detected in nasal fluid by real-time RT-PCR from 7 days post challenge. These experimental results confirmed the safety and efficacy of the attenuated PRRS vaccine based on BG895strain.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88464770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-12DOI: 10.25073/2588-1140/vnunst.5336
Doan Thi Hoang Oanh, D. T. Thủy, Nguyen Thi Giang Huong, H. T. Quỳnh, Phung Duc Hieu, Dang Minh Vu, Vu Thi Nguyet, L. Quynh, Bui Van Cuong, Bui Huyen Thuong
Microplastics (particles with a size of less than 5 mm) are a rising environmental problem. Microplastics can disseminate in the air and accumulate in sediments as well as in microorganisms and humans, due to their small size. Sediment is considered to be the major repository of microplastics, particularly those of the PE type. Microplastics in massive amounts accumulated in sediments, perhaps as a result of point sources or diffuse contamination. Microplastic contamination can spread from industrial production facilities, urban areas, agricultural areas, or the air. The current study was carried out to explore the occurrence of MPs in sediments at discharge sources by evaluating 27 sediment samples taken from 9 distinct waste sources from industrial activity locations to determine the amount of microplastic contamination in sediments at discharge sources. Microplastics with relatively high density were found in all sediment samples in this research, ranging from 2,900 to 238,200 particles/kg dw. The most prevalent microplastics detected in sediment samples at these sites were fibers and fragments, accounting for 59-94% and 6-41%, respectively. Fiber microplastics ranged in size from 1000 to 9,000 µm, whereas fragment microplastics ranged from 200,000 to 2,100,000 µm2. Microplastics with < 1000 µm and 1000-2000 µm sizes accounted for a significant portion of the total, reaching 21.05-37.84% and 39.74-61.17%, respectively. The hue of microplastic particles in sediment samples obtained was highly varied.
{"title":"Preliminary Investigation of Microplastics in Sediments from Industrial Manufacturing Waste Sources","authors":"Doan Thi Hoang Oanh, D. T. Thủy, Nguyen Thi Giang Huong, H. T. Quỳnh, Phung Duc Hieu, Dang Minh Vu, Vu Thi Nguyet, L. Quynh, Bui Van Cuong, Bui Huyen Thuong","doi":"10.25073/2588-1140/vnunst.5336","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5336","url":null,"abstract":"Microplastics (particles with a size of less than 5 mm) are a rising environmental problem. Microplastics can disseminate in the air and accumulate in sediments as well as in microorganisms and humans, due to their small size. Sediment is considered to be the major repository of microplastics, particularly those of the PE type. Microplastics in massive amounts accumulated in sediments, perhaps as a result of point sources or diffuse contamination. Microplastic contamination can spread from industrial production facilities, urban areas, agricultural areas, or the air. The current study was carried out to explore the occurrence of MPs in sediments at discharge sources by evaluating 27 sediment samples taken from 9 distinct waste sources from industrial activity locations to determine the amount of microplastic contamination in sediments at discharge sources. Microplastics with relatively high density were found in all sediment samples in this research, ranging from 2,900 to 238,200 particles/kg dw. The most prevalent microplastics detected in sediment samples at these sites were fibers and fragments, accounting for 59-94% and 6-41%, respectively. Fiber microplastics ranged in size from 1000 to 9,000 µm, whereas fragment microplastics ranged from 200,000 to 2,100,000 µm2. Microplastics with < 1000 µm and 1000-2000 µm sizes accounted for a significant portion of the total, reaching 21.05-37.84% and 39.74-61.17%, respectively. The hue of microplastic particles in sediment samples obtained was highly varied.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86144980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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