Veterinaria italiana最新文献

Extracellular vesicles (EVs) are cell-derived and play a notable role in the development of chronic diseases and can be used as biomarkers as they transport microRNAs (miRNA). Existing research has found that most miRNA functions are carried out via intercellular transmission of EVs, which can protect and sort miRNAs. Early detection of disease is crucial for controlling the spread of the disease and improving livestock prognosis. miRNAs play a promising role as circulating biomarkers for early identification of disease. miRNA expressed in paratuberculosis infection has been identified using a variety of samples, including tissue, serum, whole blood, and macrophages. Key findings state that bta-miR-150 and bta-miR-1246 were observed in macrophages, whereas miR-29a and miR-92b were detected in serum and intestinal tissue. such as miR-143 and miR-485 underscore their potential as diagnostic and prognostic biomarkers in different stages of the disease. Studies on personalized medicine involve the detection of biomarker molecules such as miRNA and modulating therapy depending on the patient's severity of the disease, applying techniques like Northern blotting, qPCR, microarrays, and NGS. The current review assesses the potential use of circulating microRNAs as biomarkers for early and accurate diagnosis of Mycobacterium avium subsp. paratuberculosis infection, where the microRNAs could even serve as a point-of-care test.

Rift Valley Fever (RVF) remains a significant public health and economic concern in Niger, particularly in the Tahoua region. This study aimed to update seroprevalence estimates of Rift Valley Fever Virus (RVFV) and identify high-risk areas and animal populations. A cross-sectional survey was conducted between January and May 2024, during which 615 domestic ruminants (cattle, sheep, goats, and camels) were sampled and tested for RVFV-specific antibodies using a competitive enzyme-linked immunosorbent assay (cELISA). The overall RVFV seroprevalence in the Tahoua region was 11.87% (±2.55%). Camels exhibited the highest seroprevalence (36.56%), followed by cattle (17.69%), while small ruminants showed much lower rates: 3.55% in goats and 3.37% in sheep. Significant geographic heterogeneity was observed, with the highest prevalence recorded in Birni N'Konni (30.53%, p < 0.05). No statistically significant differences in seroprevalence were found by sex (p = 0.909) or age (p = 0.876), although adults and females tended to have slightly higher rates. These findings confirm ongoing RVFV circulation in the region and identify camels as the most affected species. The results underscore the need for enhanced, species-specific surveillance, targeted vaccination campaigns, and vector control strategies in high-risk areas to prevent future outbreaks and protect both animal and human health.

Lumpy Skin Disease virus (LSDV) is a Capripoxvirus that causes Lumpy Skin Disease (LSD), a highly contagious disease of cattle transmitted primarily by blood-feeding arthropods, but also through direct contact and fomites. On 20 June 2025, an outbreak was reported in a beef cattle farm in Orani (Nuoro, Sardinia, Italy), where 21 of 131 animals showed typical clinical signs. Fourteen samples tested positive for LSDV by real-time PCR, and selected specimens underwent whole genome sequencing, generating three high-quality consensus sequences. Phylogenetic analysis placed the Sardinian strains within clade 1.2, closely related to a Nigerian isolate from 2018 and clearly distinct from vaccine-derived strains and those responsible for the Balkan outbreaks between 2012 and 2016. LSD outbreaks also occurred in North Africa during 2023-2024, but genomic data from those episodes are not yet available for comparison. The exact route of introduction into Italy therefore remains uncertain, with possible pathways including windborne dispersal of infected vectors or other anthropogenic activities. This first genomic characterization of LSDV in Italy highlights the need for strengthened genomic and entomological surveillance, data sharing, and integrated approaches to trace virus incursions and assess transboundary risks.

Spotted fever rickettsioses are tick-borne diseases of major epidemiological importance worldwide. Ticks can interact pathogenically with humans through endemic foci or sporadic epidemic outbreaks. Novel analytical tools and decision-making frameworks for tick-borne disease surveillance may improve resource allocation and enhance the response capacity of public health services. In this study, we characterize and model the bioclimatic suitability of three tick species-Amblyomma aureolatum, A. ovale, and A. sculptum-across Brazil. The climatic niche of each species was quantified using four modelling algorithms (BIOCLIM, GLM, Maxent, and SVM) implemented within an automated modelling workflow (modleR). A total of 585 occurrence records were retained for modelling: 55 for A. aureolatum, 120 for A. ovale, and 410 for A. sculptum, covering the entire Brazilian territory. Amblyomma sculptum exhibited a broader tolerance to temperature and precipitation variability, suggesting a wider potential distribution range compared to the other two species. This species was primarily associated with grassland habitats, such as those in the Cerrado biome. In contrast, A. aureolatum and A. ovale were more strongly associated with forested areas, particularly along the Atlantic Forest coastal region. The modelling outputs presented here provide valuable insights to support targeted health surveillance and intervention strategies for tick-borne diseases in Brazil.

Canine parvoviral enteritis (CPE) is a highly contagious and often fatal disease, particularly affecting young, unvaccinated dogs. Although anecdotal reports suggest seasonal variation in CPE incidence in Nigeria, comprehensive time series analyses remain scarce. This study analyzed clinical records of CPE cases-diagnosed either clinically or via laboratory confirmation-collected from three veterinary clinics in Ibadan, Nigeria, between January 2018 and December 2024. Temporal patterns were decomposed into trend, seasonal, and random components. Stationarity and temporal autocorrelation were assessed using the Augmented Dickey-Fuller and Ljung-Box tests, respectively. An Autoregressive Integrated Moving Average (ARIMA) model was selected using the auto.arima function implemented in R (Vienna, Austria), and subsequently used to forecast CPE incidence over a 24-month horizon. The Box-Pierce test on residuals (P = 0.9409) confirmed the model's adequacy. CPE incidence showed distinct seasonal peaks during the dry months, best captured by the ARIMA (1,1,1)(1,0,0) (model non-seasonal autoregressive order =1, differencing order =1, and moving average order =1; seasonal autoregressive order =1, differencing order = 0, and moving average order = 0; monthly seasonality = 12), and exhibited a negative correlation with historical average monthly rainfall (r = -0.55). However, forecasts for 2025-2026 suggest a gradual decline in incidence and a transition toward year-round occurrence with less pronounced seasonal peaks. These findings underscore the need for continuous preventive efforts. Veterinary practitioners should maintain a high index of suspicion for CPE, especially in young dogs with incomplete vaccination status.

Among the least explored yet highly relevant aspects of wildlife health are host-endoparasite interactions, particularly those involving helminths with zoonotic potential. Carnivores are known reservoirs of several such parasites. This study investigates the diversity of gastrointestinal helminths in small carnivores (Mammalia: Carnivora) inhabiting a tropical dry forest ecosystem in the Colombian Caribbean. A total of 60 fecal samples were collected from five species-Cerdocyon thous, Leopardus pardalis, Procyon spp., Eira barbara, and Puma yagouaroundi-within the Sanguaré Natural Reserve (Sucre, Colombia) and subjected to parasitological analysis. Eleven nematode taxa, two trematode taxa, and three forms morphologically consistent with cestodes were identified. Overall, 56.7% of the samples tested positive for at least one helminth species. The most prevalent parasite was Spirometra sp. (20%), followed by Strongyloides sp. (18%) and Toxocara sp. (13%). Notably, this study constitutes the first report of gastrointestinal helminths in Procyon spp. and Eira barbara in wild populations in Colombia. The detection of Spirometra sp. and Toxocara sp.-both genera with known zoonotic representatives-underscores the importance of monitoring parasitic infections in wild carnivores to better evaluate the potential risk of spillover to domestic animals and humans.

This study presents the first report of the molecular characterization of Anaplasma platys infection in the Bangladesh-India border region, specifically in the state of Tripura, along with its successful treatment. Anaplasma platys is a rickettsial organism transmitted by hard ticks that infest dogs, with marked thrombocytopenia and anemia being among the most important clinical manifestations, related to the formation of morulae in the platelets. A dog with a history of suspected anaplasmosis was presented for clinical investigation. Hematological analysis revealed a hemoglobin level of 6.8 g/dL, a hematocrit of 19.4%, a total red cell count of 3.5 × 10¹²/dL, a total white cell count of 7.8 × 10⁹/dL, and a platelet count of 48 × 10⁹/dL. Upon microscopic and molecular examination, A. platys was identified as the causative organism responsible for the alterations in blood parameters. Treatment involved the intravenous administration of doxycycline at a dose of 10 mg/kg body weight once daily for five days, followed by oral doxycycline tablets at the same dosage for 15 days. The dog showed gradual improvement and complete recovery within 20 days of treatment. Molecular characterization and phylogenetic inference targeting the 16S rRNA gene revealed low divergence within the species.

This study aimed to estimate the seroprevalence of bovine viral diarrhea virus (BVDV) in Moroccan cattle and to investigate associations with age, sex, breed, housing system, and geographic region. A total of 545 serum samples were randomly collected from cattle across various Moroccan regions between December 2023 and February 2024. Antibodies against BVDV were detected using a commercial competitive ELISA, and herd-level data were gathered via questionnaire. Overall, 25.0% (95% CI: 21.4 - 28.8) of samples tested seropositive. The highest prevalence was observed in the Tanger-Tetouan-Al Hoceima region (44.0%; 95% CI: 24.4 - 65.1), followed by Casablanca-Settat (32.6%; 95% CI: 26.1 - 39.6). Seropositivity was highest among cattle older than 12 months (37.5%) and in crossbred animals (86.1%) compared to purebreds (74.3%). Although not statistically significant, cattle in intensive systems showed higher prevalence (29.3%) than those in semi-intensive systems (23.2%). Females exhibited a higher seroprevalence (27.87%) than males (21.7%), with sex emerging as a significant risk factor. These findings highlight the widespread circulation of BVDV in Morocco and underscore the need for targeted control measures and improved herd management practices to mitigate virus transmission.

Neosporosis is a major cause of bovine abortion worldwide, leading to substantial economic losses. In western Türkiye, an area characterized by intensive cattle breeding, data on this disease remain limited, and no prior studies have investigated its risk factors. This study aimed to determine the prevalence, associated risk factors, and phylogenetic profile of Neospora caninum in the region. Visceral tissues from 88 aborted cattle fetuses were analyzed through genomic DNA isolation, real-time PCR (qPCR), and sequencing. In parallel, the presence of N. caninum antibodies was assessed in 973 serum samples using ELISA. N. caninum DNA was detected in 8 (9.09%) fetal samples by qPCR, with three of these further confirmed via conventional PCR and sequencing. Sequence analysis validated the presence of N. caninum in these three samples. Serologically, 122 of 973 serum samples (12.53%) tested positive for N. caninum antibodies. Statistical analysis of potential risk factors-including province, gender, age group, origin, and farm type-identified farm type as the most significant determinant of seropositivity. Notably, family-operated farms exhibited a higher prevalence of positive cases. This study represents the first large-scale investigation of N. caninum-associated reproductive losses in western Türkiye. The findings offer valuable insights for the development of targeted control and prevention strategies in affected cattle populations.

Avian reovirus (ARV) is a major causative agent of viral arthritis (VA), tenosynovitis, and malabsorption syndrome (MAS) in chickens, with significant economic consequences due to growth retardation, reduced production performance, and immunosuppression. Despite routine vaccination of breeder chickens against ARV, cases of VA and MAS continue to be reported in commercial flocks in recent years. Moreover, there is a lack of recent data on the genetic characteristics of circulating field ARV strains in India. In light of these concerns, a study was conducted to investigate the involvement of ARV in chickens exhibiting clinical signs suggestive of VA or MAS. Samples were collected from 27 commercial broiler and desi-chicken flocks across the mid-western region of Tamil Nadu, South India. Molecular confirmation was performed using reverse transcription polymerase chain reaction (RT-PCR) targeting a partial region of the σC gene within the S1 segment. Of the 27 flocks sampled, only two samples - both from desi-chickens aged two and three weeks - tested positive for ARV. Sequence analysis of these positive samples, compared against available ARV sequences in GenBank (including vaccine strains) revealed that the identified strains clustered within ARV genogroup II. This represents the first report of cluster II ARV in India, indicating the circulation of genetically distinct ARV strains in Indian poultry populations. These findings underscore the need for routine molecular surveillance of ARV genotypes in India and highlight the potential mismatch between circulating field strains and current vaccine strains. Comprehensive genotype monitoring is essential to upgrade vaccine design and implement effective control strategies for ARV-associated diseases in Indian poultry production.