Melatonin functions as a plant growth regulator, has diverse functions and plays an important role in ripening and fruit senescence. In the current study, we investigated the effect of exogenous melatonin (0.1 mM and 0.5 mM) on reactive oxygen species (ROS) metabolism, membrane lipid peroxidation and antioxidant enzyme activity of avocado (Persea americana Mill. cv. 034) during fruit ripening at 22oC ± 1 and 75-80% relative humidity (RH). The results showed that postharvest fruits treated with 0.5 mM melatonin effectively reduced the accumulation of superoxide anion (O2.), hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the mesocarp of the fruit. In addition, melatonin treatment also significantly promoted the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in avocado. It is suggested that enhanced antioxidant enzyme activity induced by melatonin treatment may contribute to scavenge ROS and alleviating membrane lipid peroxidation in avocado fruit. The results indicate that the MT application might collectively contribute to the delay senescence and maintain postharvest quality of avocado.
{"title":"Effect of exogenous melatonin on antioxidant enzyme activities and membrane lipid peroxidation in avocado fruit during ripening","authors":"To Thi Bich Ngoc, Vo Minh Thu, Nguyen Thi Phuong Hien, Huynh Thi Thanh Tra, Hoang Duc Anh, Truong Thi Hue","doi":"10.15625/1811-4989/17191","DOIUrl":"https://doi.org/10.15625/1811-4989/17191","url":null,"abstract":"Melatonin functions as a plant growth regulator, has diverse functions and plays an important role in ripening and fruit senescence. In the current study, we investigated the effect of exogenous melatonin (0.1 mM and 0.5 mM) on reactive oxygen species (ROS) metabolism, membrane lipid peroxidation and antioxidant enzyme activity of avocado (Persea americana Mill. cv. 034) during fruit ripening at 22oC ± 1 and 75-80% relative humidity (RH). The results showed that postharvest fruits treated with 0.5 mM melatonin effectively reduced the accumulation of superoxide anion (O2.), hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the mesocarp of the fruit. In addition, melatonin treatment also significantly promoted the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in avocado. It is suggested that enhanced antioxidant enzyme activity induced by melatonin treatment may contribute to scavenge ROS and alleviating membrane lipid peroxidation in avocado fruit. The results indicate that the MT application might collectively contribute to the delay senescence and maintain postharvest quality of avocado.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86952581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/15982
Nguyen Thi Binh, Nguyen Thi Quy, Do Thi Huyen, Le Thi Thu Hong, Truong Nam Hai
Beta-glucosidase (BGL) is an enzyme involved in the hydrolysis of cellulose and plays an important role in many biological processes. This enzyme is widely available in animals, plants, and microorganisms. Expression of the recombinant enzyme in Escherichia coli is considered a suitable choice for high production via a fast growth rate and feasible manipulation. In this study, we optimised some conditions for the high soluble production of recombinant beta-glucosidase whose gene sequence was mined from the DNA metagenome of Cuc Phuong humus surrounding white rot fungi. The gene bgl was cloned in pET 22b(+) and expressed in the E. coli Rosetta 1 strain. Production of the recombinant BGL was examined at temperature ranges from 18 to 37oC. The recombinant BGL was obtained as a soluble form at low temperatures, and the optimal temperature was 25°C. In comparison to LB, TB, SB, PE media, mTB rich medium in presence of glucose produced the most BGL. Besides, the results assessing the inducer condition showed that the best IPTG concentration for producing the BGL was 0.3 mM IPTG. Furthermore, some fermentation conditions affecting the level of BGL production were assessed as the induction point and harvesting time. The BGL production was the most suitable when the cell at mid-log phase with an OD600 1 and harvesting time after 4 hours of induction. Importantly, the recombinant BGL had good activity on the esculin substrate plate. Based on the selected conditions, the recombinant BGL could be produced high amount to facilitate for future purification and characterization of the recombinant BGL.
{"title":"Selection of optimal culture conditions for expression of recombinant beta-glucosidase in Escherichia coli","authors":"Nguyen Thi Binh, Nguyen Thi Quy, Do Thi Huyen, Le Thi Thu Hong, Truong Nam Hai","doi":"10.15625/1811-4989/15982","DOIUrl":"https://doi.org/10.15625/1811-4989/15982","url":null,"abstract":"Beta-glucosidase (BGL) is an enzyme involved in the hydrolysis of cellulose and plays an important role in many biological processes. This enzyme is widely available in animals, plants, and microorganisms. Expression of the recombinant enzyme in Escherichia coli is considered a suitable choice for high production via a fast growth rate and feasible manipulation. In this study, we optimised some conditions for the high soluble production of recombinant beta-glucosidase whose gene sequence was mined from the DNA metagenome of Cuc Phuong humus surrounding white rot fungi. The gene bgl was cloned in pET 22b(+) and expressed in the E. coli Rosetta 1 strain. Production of the recombinant BGL was examined at temperature ranges from 18 to 37oC. The recombinant BGL was obtained as a soluble form at low temperatures, and the optimal temperature was 25°C. In comparison to LB, TB, SB, PE media, mTB rich medium in presence of glucose produced the most BGL. Besides, the results assessing the inducer condition showed that the best IPTG concentration for producing the BGL was 0.3 mM IPTG. Furthermore, some fermentation conditions affecting the level of BGL production were assessed as the induction point and harvesting time. The BGL production was the most suitable when the cell at mid-log phase with an OD600 1 and harvesting time after 4 hours of induction. Importantly, the recombinant BGL had good activity on the esculin substrate plate. Based on the selected conditions, the recombinant BGL could be produced high amount to facilitate for future purification and characterization of the recombinant BGL.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86806680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/17056
Xuan Thach Tran, Thi-Hien Vu, Thi Thu Ha, Thi Hoa Nguyen, The Hung Hoang, Duc Hoang Le, Quyen Van Dong, N. Trung Nguyen, Thi Tuyet Mai Nguyen
Hypercholesterolemia is a major cause of cholesterol build-up in the coronary arteries, which can subsequently lead to heart disease or atherosclerosis. Cholesterol levels can be lowered by cholesterol-lowering drugs but some of these drugs may have harmful side effects, while supplementation of Lactobacillus has shown the potential to reduce serum cholesterol levels by virtue of bile salt hydrolase (bsh) activity. In this study, Lactobacillus plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14, had been isolated from Vietnamese healthy adults, were able to deconjugate glycodeoxycholate (GDC) on MRS plates and MRS broth supplemented with GDC. In addition, deconjugating activity of L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 were also found in cell-free extract as expressed by amount of glycine that released in the supernatant. Four bsh genes including bsh1, bsh2, bsh3, and bsh4 have been identified by PCR in these strains. In addition, L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 also showed high ability to resist bile salts and low pH. The results of 16S rRNA gene analyses showed that L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 and had high similarity scores with L. plantarum ZZU 23 (100%), L. rhamnosus JCM 1136 (99%) and L. plantarum S7 (98.65%), respectively. This study suggests that L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 have the potential to be explored as probiotics in the management of hypercholesterolaemia in near future.
高胆固醇血症是冠状动脉中胆固醇积聚的主要原因，从而导致心脏病或动脉粥样硬化。降胆固醇药物可以降低胆固醇水平，但其中一些药物可能有有害的副作用，而补充乳酸杆菌已显示出通过胆汁盐水解酶(bsh)活性降低血清胆固醇水平的潜力。本研究从越南健康成人中分离到植物乳杆菌VFE-04、鼠李糖乳杆菌VFE-08和植物乳杆菌VFE-14，在MRS平板和添加GDC的MRS肉汤上均能解结糖脱氧巧克力酸(GDC)。此外，植物乳杆菌VFE-04、鼠李糖乳杆菌VFE-08和植物乳杆菌VFE-14的解结活性也以上清液中释放的甘氨酸量表示。PCR鉴定出bsh1、bsh2、bsh3和bsh4 4个bsh基因。此外，L. plantarum VFE-04、L. rhamnosus VFE-08和L. plantarum VFE-14也表现出较高的抗胆盐和低ph的能力。16S rRNA基因分析结果表明，L. plantarum VFE-04、L. rhamnosus VFE-08和L. plantarum VFE-14分别与L. plantarum ZZU 23(100%)、L. rhamnosus JCM 1136(99%)和L. plantarum S7(98.65%)具有较高的相似度。本研究提示植物乳杆菌VFE-04、鼠李糖乳杆菌VFE-08和植物乳杆菌VFE-14有潜力在不久的将来作为益生菌用于治疗高胆固醇血症。
{"title":"Screening bile salt hydrolase activity of Lactobacillus isolated from Vietnamese human origins","authors":"Xuan Thach Tran, Thi-Hien Vu, Thi Thu Ha, Thi Hoa Nguyen, The Hung Hoang, Duc Hoang Le, Quyen Van Dong, N. Trung Nguyen, Thi Tuyet Mai Nguyen","doi":"10.15625/1811-4989/17056","DOIUrl":"https://doi.org/10.15625/1811-4989/17056","url":null,"abstract":"Hypercholesterolemia is a major cause of cholesterol build-up in the coronary arteries, which can subsequently lead to heart disease or atherosclerosis. Cholesterol levels can be lowered by cholesterol-lowering drugs but some of these drugs may have harmful side effects, while supplementation of Lactobacillus has shown the potential to reduce serum cholesterol levels by virtue of bile salt hydrolase (bsh) activity. In this study, Lactobacillus plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14, had been isolated from Vietnamese healthy adults, were able to deconjugate glycodeoxycholate (GDC) on MRS plates and MRS broth supplemented with GDC. In addition, deconjugating activity of L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 were also found in cell-free extract as expressed by amount of glycine that released in the supernatant. Four bsh genes including bsh1, bsh2, bsh3, and bsh4 have been identified by PCR in these strains. In addition, L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 also showed high ability to resist bile salts and low pH. The results of 16S rRNA gene analyses showed that L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 and had high similarity scores with L. plantarum ZZU 23 (100%), L. rhamnosus JCM 1136 (99%) and L. plantarum S7 (98.65%), respectively. This study suggests that L. plantarum VFE-04, L. rhamnosus VFE-08, and L. plantarum VFE-14 have the potential to be explored as probiotics in the management of hypercholesterolaemia in near future.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79757991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16373
Le Hoang Duc, Nguyen Trung Nam, Dang Thi Hoang Oanh, Tran Trung Thanh, Chu Hoang Ha
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has caused severe damage to the shrimp farming industry of Vietnam. Probiotics are chosen as a prophylactic method to mitigate the outbreak of diseases. We report in this article the isolation and evaluation of the effect of a potential probiotic, Bacillus subtilis BLD01, which enhances the survival rates and changes gut microbiota of whiteleg shrimp (Penaeus vannamei) after challenge with AHPND V. parahaemolyticus. After seven days of the challenge, the treatment where shrimps were fed with B. subtilis BLD01 strain (106 CFU/g) and challenge with AHPND V. parahaemolyticus (106 CFU/mL) showed high survival rates of 71% as compared to 33% in the treatment where shrimp were given standard feed without probiotics supplementation and challenged with AHPND V. parahaemolyticus. 16S rRNA amplicon data of the gut microbiomes of shrimps in four treatments were carried out using Illumina sequencing. A total of 231 436 reads were obtained, and a total of 14 phyla, 28 classes, 142 genera were revealed. The most abundant phyla in all subjects were Proteobacteria and Bacteroidetes. The sequence number of the Vibrio genus was the highest with 28% in treatment without B. subtillis BLD01 in-feed addition and shrimps challenged with AHPND V. parahaemolyticus. The sequence number of Bacillus genus was the highest with 3% in the treatment with B. subtillis BLD01 addition and without AHPND V. parahaemolyticus challenge. These results contribute to confirming the mechanism of action of B. subtilis against V. parahaemolyticus in the experimental model, creating a scientific basis for the development and use of probiotics products applied in shrimp farming in Vietnam.
{"title":"Isolation and evaluation the effect of Bacillus subtillis BLD01 strain on the survival rates and gut microbiota of Penaeus vannamei after challenge with Vibrio parahaemolyticus","authors":"Le Hoang Duc, Nguyen Trung Nam, Dang Thi Hoang Oanh, Tran Trung Thanh, Chu Hoang Ha","doi":"10.15625/1811-4989/16373","DOIUrl":"https://doi.org/10.15625/1811-4989/16373","url":null,"abstract":"Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has caused severe damage to the shrimp farming industry of Vietnam. Probiotics are chosen as a prophylactic method to mitigate the outbreak of diseases. We report in this article the isolation and evaluation of the effect of a potential probiotic, Bacillus subtilis BLD01, which enhances the survival rates and changes gut microbiota of whiteleg shrimp (Penaeus vannamei) after challenge with AHPND V. parahaemolyticus. After seven days of the challenge, the treatment where shrimps were fed with B. subtilis BLD01 strain (106 CFU/g) and challenge with AHPND V. parahaemolyticus (106 CFU/mL) showed high survival rates of 71% as compared to 33% in the treatment where shrimp were given standard feed without probiotics supplementation and challenged with AHPND V. parahaemolyticus. 16S rRNA amplicon data of the gut microbiomes of shrimps in four treatments were carried out using Illumina sequencing. A total of 231 436 reads were obtained, and a total of 14 phyla, 28 classes, 142 genera were revealed. The most abundant phyla in all subjects were Proteobacteria and Bacteroidetes. The sequence number of the Vibrio genus was the highest with 28% in treatment without B. subtillis BLD01 in-feed addition and shrimps challenged with AHPND V. parahaemolyticus. The sequence number of Bacillus genus was the highest with 3% in the treatment with B. subtillis BLD01 addition and without AHPND V. parahaemolyticus challenge. These results contribute to confirming the mechanism of action of B. subtilis against V. parahaemolyticus in the experimental model, creating a scientific basis for the development and use of probiotics products applied in shrimp farming in Vietnam.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81142692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.15625/1811-4989/16645
Nguyen Tran Dinh, Le Phuong Thu, Ngo Thanh Dat, Nguyen Khanh Toan, Pham Le Anh
Nature-based wastewater treatment employing microalgae and bacteria has gained serious attention due to its combination with valuable biomass production. In wastewater, microalgae serves as the primary source of dissolved oxygen (DO) production for bacterial organic matter degradation. In addition, microalgae can effectively remove nutrients, pathogens as well as various heavy metals. Bacteria, on the other hand, has been widely applied in biological wastewater treatment for stabilization of organic matter, nitrification, denitrification, and under some specific conditions, enhanced biological phosphorus removal. When cultured together, microalgae and bacteria can cooperate effectively for wastewater treatment as well as form big flocs which can be harvested easily via sedimentation. However, some natural antagonistic interactions between them should be expected. Various environmental and operational factors showed significant influences on microalgae and bacteria in wastewater. They can impact system performance individually or in combination with others. Therefore, those factors should be carefully monitored for improving performance of the system.
{"title":"The roles of microalgae and bacteria in wastewater treatment","authors":"Nguyen Tran Dinh, Le Phuong Thu, Ngo Thanh Dat, Nguyen Khanh Toan, Pham Le Anh","doi":"10.15625/1811-4989/16645","DOIUrl":"https://doi.org/10.15625/1811-4989/16645","url":null,"abstract":"Nature-based wastewater treatment employing microalgae and bacteria has gained serious attention due to its combination with valuable biomass production. In wastewater, microalgae serves as the primary source of dissolved oxygen (DO) production for bacterial organic matter degradation. In addition, microalgae can effectively remove nutrients, pathogens as well as various heavy metals. Bacteria, on the other hand, has been widely applied in biological wastewater treatment for stabilization of organic matter, nitrification, denitrification, and under some specific conditions, enhanced biological phosphorus removal. When cultured together, microalgae and bacteria can cooperate effectively for wastewater treatment as well as form big flocs which can be harvested easily via sedimentation. However, some natural antagonistic interactions between them should be expected. Various environmental and operational factors showed significant influences on microalgae and bacteria in wastewater. They can impact system performance individually or in combination with others. Therefore, those factors should be carefully monitored for improving performance of the system.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"241 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77479278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Owing to their sessile nature, plants are easily affected by various extenal factors. Among those, drought and salinity are considered as the most common stresses, which often pose a threat to plant growth and development. Major effects of the drought and salinity are interconnected and drive similar series of molecular changes in plants. These alterations in response to the stress are under the regulation of various signaling pathways, including the engangement of evolutionarily conserved two-component systems (TCSs). Three components with distinct functions can be found in a functional TCS, which are histidine kinases (HKs), histidine-containing phosphotransfer proteins (HPts), and response regulator proteins (RRs). Previous research revealed that the soybean (Glycine max) GmRR34 acts as an important regulatory protein in plants under drought stress conditions. In this project, the investigation on the role of GmRR34 in osmotic stress responses was extended to salinity by examining the expression of a subset of salinity-responsive genes using RT-qPCR method. Our analyses showed that the transgenic Arabidopsis plants ectopically expressing GmRR34 displayed enhanced expression of several important stress-related genes, including Catalase 1 (CAT1), Stromal ascorbate peroxidase 1 (sAPX1), Copper/zinc superoxide dismutase 1 (CSD1), Sodium/hydrogen exchanger 1 (NHX1) and Salt overly sensitive 2 (SOS2). These results indicate that GmRR34-transgenic plants might be more salt-tolerant thanks to stronger activities of antioxidant enzymes and better capacity in maintaining cytosolic ion homeostasis. Therefore, it is highlighted the necessity to perform further studies to fully characterize the GmRR34 biological functions as well as explore its application potential in enhancing the salt tolerance of crop plants.
{"title":"Expression study of stress-related genes in salinity-treated transgenic Arabidopsis harboring soybean Response Regulator 34","authors":"Pham Ngoc Thai Huyen, Hoang Thi Lan Xuan, Nguyen Nguyen Chuong, Nguyen Thi Phuong Thao","doi":"10.15625/1811-4989/16149","DOIUrl":"https://doi.org/10.15625/1811-4989/16149","url":null,"abstract":"Owing to their sessile nature, plants are easily affected by various extenal factors. Among those, drought and salinity are considered as the most common stresses, which often pose a threat to plant growth and development. Major effects of the drought and salinity are interconnected and drive similar series of molecular changes in plants. These alterations in response to the stress are under the regulation of various signaling pathways, including the engangement of evolutionarily conserved two-component systems (TCSs). Three components with distinct functions can be found in a functional TCS, which are histidine kinases (HKs), histidine-containing phosphotransfer proteins (HPts), and response regulator proteins (RRs). Previous research revealed that the soybean (Glycine max) GmRR34 acts as an important regulatory protein in plants under drought stress conditions. In this project, the investigation on the role of GmRR34 in osmotic stress responses was extended to salinity by examining the expression of a subset of salinity-responsive genes using RT-qPCR method. Our analyses showed that the transgenic Arabidopsis plants ectopically expressing GmRR34 displayed enhanced expression of several important stress-related genes, including Catalase 1 (CAT1), Stromal ascorbate peroxidase 1 (sAPX1), Copper/zinc superoxide dismutase 1 (CSD1), Sodium/hydrogen exchanger 1 (NHX1) and Salt overly sensitive 2 (SOS2). These results indicate that GmRR34-transgenic plants might be more salt-tolerant thanks to stronger activities of antioxidant enzymes and better capacity in maintaining cytosolic ion homeostasis. Therefore, it is highlighted the necessity to perform further studies to fully characterize the GmRR34 biological functions as well as explore its application potential in enhancing the salt tolerance of crop plants. ","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81641242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.15625/1811-4989/16117
Nguyen Manh Tuan, Le Quang Vien, Truong Phuc Hung, Tran Minh Quan, Do Thi Thu Hien, Do Bich Due, Duong Thi Khuyen
During the recent decade, uncultured bacteria have been special interest as potential candidates for discovering novel antibacterial compounds. Two strains C101 and C102 were negative Gram bacteria, only growing on low nutrient media as R2A/3, NB/3, LB/10 and R4/10 compared to the usual. On R2A/3 medium, colonies of the isolates were round, convex, lemon yellow color with the size of 1-1.5 mm after six days of incubation at 28oC. Cells were 0.2-0.3 × 0.8-1.3 µm. The strains C101 and C102 were able to grow at temperature ranging 15-37oC (optimum at 25-28oC), pH 5-8 (optimum in pH 6-7). The sequences of 16S rRNA genes from strain C101 (MT756087) and C102 (MT756088) shared 100% identity. Analysis of full-length 16S rRNA gene sequence of strain C101 via using NCBI Blast, EzTaxon Database revealed the highest similarity of 99.18-100% to uncultured clones, and 97.86% to type species as Parasegetibacter terrae SGM2-10T. Genetic sequence analysis data showed that strain C101 should be considered a novel candidate species of the genus Parasegetibacter. Antibacterial compound was extracted from culture of strain C101 in R4/10 medium for ten days of shaking incubator at 28oC and exhibited susceptible activity to inhibit Bacillus anthracis KEMB 211-146 at a concentration of 2 µg/L and Staphylococcus aureus ATCC 6538 at 4 µg/L; intermediate inhibiting Bacillus subtilis KEMB 51201-001 at 8 µg/L, Staphylococcus epidermidis ATCC 14990 at 8 µg/L, and S. aureus CCARM 3155 at 16 µg/L; inhibition of S. aureus CCARM 3095 at 64 µg/L, S. aureus CCARM 3192 at 32 µg/L, and S. epidermidis CCARM 3710 at 64 µg/L.
{"title":"Identification, cultivation condition of Parasevetibacter sp C101 and antimicrobial activity of the bacterial culture extract","authors":"Nguyen Manh Tuan, Le Quang Vien, Truong Phuc Hung, Tran Minh Quan, Do Thi Thu Hien, Do Bich Due, Duong Thi Khuyen","doi":"10.15625/1811-4989/16117","DOIUrl":"https://doi.org/10.15625/1811-4989/16117","url":null,"abstract":"During the recent decade, uncultured bacteria have been special interest as potential candidates for discovering novel antibacterial compounds. Two strains C101 and C102 were negative Gram bacteria, only growing on low nutrient media as R2A/3, NB/3, LB/10 and R4/10 compared to the usual. On R2A/3 medium, colonies of the isolates were round, convex, lemon yellow color with the size of 1-1.5 mm after six days of incubation at 28oC. Cells were 0.2-0.3 × 0.8-1.3 µm. The strains C101 and C102 were able to grow at temperature ranging 15-37oC (optimum at 25-28oC), pH 5-8 (optimum in pH 6-7). The sequences of 16S rRNA genes from strain C101 (MT756087) and C102 (MT756088) shared 100% identity. Analysis of full-length 16S rRNA gene sequence of strain C101 via using NCBI Blast, EzTaxon Database revealed the highest similarity of 99.18-100% to uncultured clones, and 97.86% to type species as Parasegetibacter terrae SGM2-10T. Genetic sequence analysis data showed that strain C101 should be considered a novel candidate species of the genus Parasegetibacter. Antibacterial compound was extracted from culture of strain C101 in R4/10 medium for ten days of shaking incubator at 28oC and exhibited susceptible activity to inhibit Bacillus anthracis KEMB 211-146 at a concentration of 2 µg/L and Staphylococcus aureus ATCC 6538 at 4 µg/L; intermediate inhibiting Bacillus subtilis KEMB 51201-001 at 8 µg/L, Staphylococcus epidermidis ATCC 14990 at 8 µg/L, and S. aureus CCARM 3155 at 16 µg/L; inhibition of S. aureus CCARM 3095 at 64 µg/L, S. aureus CCARM 3192 at 32 µg/L, and S. epidermidis CCARM 3710 at 64 µg/L.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86771176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.15625/1811-4989/16984
Dao Manh Cuong, Phan Thi Thu Hien, Bui Van Ngoc
The metagenomics approach is quickly developing in researchs about the composition of a microbial community. In the past few decades, antibiotic resistance is increasingly popular together with emerging diseases while repeated isolation of known secondary metabolites and a decline of novel compounds in the terrestrial environment present an urgent requirement more and more in the discovery of novel marine bioactive compounds. Paired-end reads of 16S rRNA sequence of bacteria living in the coral Acropora millepora obtained by the Illumina next-generation sequencing technology to be processed by DADA2 pipeline, phyloseq, and ggplot2 packages showed that microbial composition has transformation, specifically, bleached coral mucus has an alpha diversity to be higher than healthy coral mucus. Moreover, healthy and bleached coral mucus is also used to evaluate antibacterial activity on the pandemic strain of Vibrio parahaemolyticus and identify the ability of cell cytotoxicity to HCT116 colon cancer cells. The coral surface mucus layer (SML) of healthy coral exhibited the ability of inhibition to the growth of the disease strain is higher than that of bleached coral at all concentrations (10 – 30 µL). The cytotoxicity of colon cancer cell line HCT116 was also clearly observed when treated with healthy SML. Comparison of cytotoxicity of two mucus types shows that healthy coral mucus has inhibition to colon cancer cell line to be 1.5 times higher than mucus is taken from bleached coral. The composition of the microbial community changes when corals transfer from a healthy state to a bleached one. Consequently, antibacterial activity and cytotoxicity also come down.
{"title":"CHANGES IN MICROBIAL COMMUNITY COMPOSITION AFFECT BIOACTIVITIES OF MUCUS ISOLATED FROM CORAL ACROPORA MILLEPORA","authors":"Dao Manh Cuong, Phan Thi Thu Hien, Bui Van Ngoc","doi":"10.15625/1811-4989/16984","DOIUrl":"https://doi.org/10.15625/1811-4989/16984","url":null,"abstract":"The metagenomics approach is quickly developing in researchs about the composition of a microbial community. In the past few decades, antibiotic resistance is increasingly popular together with emerging diseases while repeated isolation of known secondary metabolites and a decline of novel compounds in the terrestrial environment present an urgent requirement more and more in the discovery of novel marine bioactive compounds. Paired-end reads of 16S rRNA sequence of bacteria living in the coral Acropora millepora obtained by the Illumina next-generation sequencing technology to be processed by DADA2 pipeline, phyloseq, and ggplot2 packages showed that microbial composition has transformation, specifically, bleached coral mucus has an alpha diversity to be higher than healthy coral mucus. Moreover, healthy and bleached coral mucus is also used to evaluate antibacterial activity on the pandemic strain of Vibrio parahaemolyticus and identify the ability of cell cytotoxicity to HCT116 colon cancer cells. The coral surface mucus layer (SML) of healthy coral exhibited the ability of inhibition to the growth of the disease strain is higher than that of bleached coral at all concentrations (10 – 30 µL). The cytotoxicity of colon cancer cell line HCT116 was also clearly observed when treated with healthy SML. Comparison of cytotoxicity of two mucus types shows that healthy coral mucus has inhibition to colon cancer cell line to be 1.5 times higher than mucus is taken from bleached coral. The composition of the microbial community changes when corals transfer from a healthy state to a bleached one. Consequently, antibacterial activity and cytotoxicity also come down.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79498202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.15625/1811-4989/16344
Nguyen Thi Nha Trang, Huynh Thi Thu Ha, Nguyen Thi Phuong Thao, Duong Thi Anh Tho, C. Trang, Le Thi Ha Thanh, Nguyen Hoang Tue, Nguyen Hoang Loc, Nguyen Ngoc Luong
Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli). The gene was cloned into two expression vectors, pQE30 and pColdII and the resulting recombinant vectors were transformed into E. coli M15 and BL21 De3 RIL codon plus strains, respectively. The expression levels of functional eGFP showed a temperature dependent pattern, in which lowering the induction temperature increased the amount of functional eGFP. Surprisingly, eGFP showed a phenomenon called auto-induction when E. coli TOP10 cells carrying recombinant pQE30 and pColdII were grown on Luria Broth plates. The recombinant eGFP showed robust stability even at room temperature, thus greatly facilitated its purification and handling. Mouse polyclonal antibodies were conveniently generated against the protein. Besides its potential application as a reporter gene in E. coli, the gene and its expression systems reported here are extremely useful as models for teaching recombinant DNA technology at undergraduate level.
{"title":"Expression of a synthetic gene encoding the enhanced green fluorescent protein in various Escherichia coli strains","authors":"Nguyen Thi Nha Trang, Huynh Thi Thu Ha, Nguyen Thi Phuong Thao, Duong Thi Anh Tho, C. Trang, Le Thi Ha Thanh, Nguyen Hoang Tue, Nguyen Hoang Loc, Nguyen Ngoc Luong","doi":"10.15625/1811-4989/16344","DOIUrl":"https://doi.org/10.15625/1811-4989/16344","url":null,"abstract":"Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli). The gene was cloned into two expression vectors, pQE30 and pColdII and the resulting recombinant vectors were transformed into E. coli M15 and BL21 De3 RIL codon plus strains, respectively. The expression levels of functional eGFP showed a temperature dependent pattern, in which lowering the induction temperature increased the amount of functional eGFP. Surprisingly, eGFP showed a phenomenon called auto-induction when E. coli TOP10 cells carrying recombinant pQE30 and pColdII were grown on Luria Broth plates. The recombinant eGFP showed robust stability even at room temperature, thus greatly facilitated its purification and handling. Mouse polyclonal antibodies were conveniently generated against the protein. Besides its potential application as a reporter gene in E. coli, the gene and its expression systems reported here are extremely useful as models for teaching recombinant DNA technology at undergraduate level. ","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86952577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.15625/1811-4989/16677
Nguyen Thi Nga, H. Thu, Nguyen Thi Tuyet Hoa, V. Hien, Nguyen Thu Trang, Nguyen Thanh Ba, Tran Van Khanh, Nguyen Huu Vu, Dong Van Quyen, To Long Thanh, Dinh Duy Khang
The attenuated porcine reproductive and respiratory syndrome virus (PRRSV) strain Hanvet1.vn was developed by Hanvet Pharmaceutical Co., Ltd. by inoculating the virulent strain 02HYon Marc-145 cells for 80 generations and used to produce PRRS vaccine. In this study, we published the results of sequencing, analyzing and comparing the genome of the attenuated PRRSV strain Hanvet1.vn compared with the original pathogenic strain 02HY. The genomes of strains Hanvet1.vn and 02HY have 8 reading frames, coding for 8 non-structural and structural proteins: NSP1a, NSP1b, GP2, GP3, GP4, GP5, MP, NP. After sequencing and translating into proteins, the gene sequence of each open reading frame (ORF) of strain Hanvet1.vn was compared with the sequence of pathogenic strain 02HY to find nucleotide and amino acid changes. The results showed that the Hanvet1.vn pathogenic strain genome (Genbank Accession KU842720) when compared with the pathogenic strain 02HY genome (Submission2490633) had89 nucleotide mutations that changed 51 amino acids in 7 ORFs and 7 proteins, respectively. Particularly, ORF6 encoding for the M protein is completely unchanged. The size of each reading frame is also exactly the same. It showed that there were no insertion and deletion (Indel) mutations in the ORFs of the attenuated strain after 80 generations of inoculation. There was a change in the genome that made the strain Hanvet1.vn become attenuated, but the gene encoding for the GP5 protein that induces the production of neutralizing antibodies only changed two nucleotides at position 471 (A->G), causing the TCA codon to turn into a TCG codon. This is a silent mutation and both codons code for the amino acid Serine (S). The second mutation at position 587 (A->T) causes Glutamine (Q) to transform into Leucine (L). However, this modification does not belong to the GP5 antigenic epitopes. In clonclusion, after 80 passages, despite changes occurred in genes of Hanvet1.vn strain for becoming an attenuated strain, the GP5 protein of the attenuated strain did not change its antigenic amino acids.
{"title":"Assessment of the genetic changes of the attenuated Hanvet1.vn strain compared with original virulent 02HY strain of the porcine reproductive and respiratory syndrome virus","authors":"Nguyen Thi Nga, H. Thu, Nguyen Thi Tuyet Hoa, V. Hien, Nguyen Thu Trang, Nguyen Thanh Ba, Tran Van Khanh, Nguyen Huu Vu, Dong Van Quyen, To Long Thanh, Dinh Duy Khang","doi":"10.15625/1811-4989/16677","DOIUrl":"https://doi.org/10.15625/1811-4989/16677","url":null,"abstract":"The attenuated porcine reproductive and respiratory syndrome virus (PRRSV) strain Hanvet1.vn was developed by Hanvet Pharmaceutical Co., Ltd. by inoculating the virulent strain 02HYon Marc-145 cells for 80 generations and used to produce PRRS vaccine. In this study, we published the results of sequencing, analyzing and comparing the genome of the attenuated PRRSV strain Hanvet1.vn compared with the original pathogenic strain 02HY. The genomes of strains Hanvet1.vn and 02HY have 8 reading frames, coding for 8 non-structural and structural proteins: NSP1a, NSP1b, GP2, GP3, GP4, GP5, MP, NP. After sequencing and translating into proteins, the gene sequence of each open reading frame (ORF) of strain Hanvet1.vn was compared with the sequence of pathogenic strain 02HY to find nucleotide and amino acid changes. The results showed that the Hanvet1.vn pathogenic strain genome (Genbank Accession KU842720) when compared with the pathogenic strain 02HY genome (Submission2490633) had89 nucleotide mutations that changed 51 amino acids in 7 ORFs and 7 proteins, respectively. Particularly, ORF6 encoding for the M protein is completely unchanged. The size of each reading frame is also exactly the same. It showed that there were no insertion and deletion (Indel) mutations in the ORFs of the attenuated strain after 80 generations of inoculation. There was a change in the genome that made the strain Hanvet1.vn become attenuated, but the gene encoding for the GP5 protein that induces the production of neutralizing antibodies only changed two nucleotides at position 471 (A->G), causing the TCA codon to turn into a TCG codon. This is a silent mutation and both codons code for the amino acid Serine (S). The second mutation at position 587 (A->T) causes Glutamine (Q) to transform into Leucine (L). However, this modification does not belong to the GP5 antigenic epitopes. In clonclusion, after 80 passages, despite changes occurred in genes of Hanvet1.vn strain for becoming an attenuated strain, the GP5 protein of the attenuated strain did not change its antigenic amino acids.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80071250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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