Chlorophyll stability index of eight subaerial blue-green algal species, collected from their natural habitats, i.e., bark of trees, soil and roof-tops and from cultures, has been determined. The algae from natural habitats showed greater chlorophyll stability compared to those algae from cultures. Among the natural algae, high chlorophyll stability was observed in the algae inhabiting adverse habitats. A slight modification in the method of Murty and Majumder (1962) for determination of chlorophyll stability index has been made. Here the total percentage loss in the amount of Chl alpha of heated sample in relation to its unheated sample has been considered as chlorophyll stability index.
{"title":"Effect of temperature on chlorophyll stability of some subaerial blue-green algae.","authors":"S N Tripathi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chlorophyll stability index of eight subaerial blue-green algal species, collected from their natural habitats, i.e., bark of trees, soil and roof-tops and from cultures, has been determined. The algae from natural habitats showed greater chlorophyll stability compared to those algae from cultures. Among the natural algae, high chlorophyll stability was observed in the algae inhabiting adverse habitats. A slight modification in the method of Murty and Majumder (1962) for determination of chlorophyll stability index has been made. Here the total percentage loss in the amount of Chl alpha of heated sample in relation to its unheated sample has been considered as chlorophyll stability index.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 7","pages":"443-6"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17475998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The beta-lactamase-inhibiting activity of 6m-ethyl-pyrid-2-yl-ammine palladium-dichloride (Pd 25681) and cis-dichloro-diammine-platinum(II) was studied and compared with the enzyme inhibitory action of potassium clavulanate and the penicillanic acid sulfone CP 45899. Using the nitrocefin test method and the Titertek/Microtiter equipment CP 45899 and potassium clavulanate were the strongest inhibitors of the Bacillus cereus beta-lactamase. Cis-dichloro-diammine-platinum(II) was fourfold less active than the palladium complex PD 25681 in äquimolar concentration. The following ID50 values were found: CP 45899: 0.0281 microgram; K-clavulanate: 0.1274 microgram; Pd 25681: 3.8603 microgram; cis-dichlorodiammine-platinum(II): 12.5120 microgram/100 microliter.
{"title":"[Biological effects of coordination compounds of transitional metals. The beta-lactamase inhibitory effect of cis-platin, 2-aminopyridine palladium chloride, clavulanic acid and penicillanic acid sulfonate CP 45,899 in the nitrocefin test and Titertek/microtiter automatic system].","authors":"W F Fleck, B Heyn, H P Schröer","doi":"10.1002/jobm.3630230506","DOIUrl":"https://doi.org/10.1002/jobm.3630230506","url":null,"abstract":"<p><p>The beta-lactamase-inhibiting activity of 6m-ethyl-pyrid-2-yl-ammine palladium-dichloride (Pd 25681) and cis-dichloro-diammine-platinum(II) was studied and compared with the enzyme inhibitory action of potassium clavulanate and the penicillanic acid sulfone CP 45899. Using the nitrocefin test method and the Titertek/Microtiter equipment CP 45899 and potassium clavulanate were the strongest inhibitors of the Bacillus cereus beta-lactamase. Cis-dichloro-diammine-platinum(II) was fourfold less active than the palladium complex PD 25681 in äquimolar concentration. The following ID50 values were found: CP 45899: 0.0281 microgram; K-clavulanate: 0.1274 microgram; Pd 25681: 3.8603 microgram; cis-dichlorodiammine-platinum(II): 12.5120 microgram/100 microliter.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 5","pages":"313-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17374155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1983-01-01DOI: 10.1002/JOBM.19830230715
J. Zindulis, H. Ehrlich
Manganese oxidation by cell suspensions and cell extracts of a freshwater bacterium, designated strain FMn 1, was investigated. Manganese appeared to be oxidized in the periplasmic space. A conventional, membrane-bound-electron transport system was not utilized. An enzyme or enzyme complex and a cofactor, each of different molecular size, were located in different parts of the cell envelope. Results suggest that the cofactor reacts with manganese in the periplasmic space and that in the presence of oxygen it is reoxidized by the enzyme. The enzyme is probably loosely bound to the membrane. A combination of enzyme and cofactor in a crude preparation exhibited a pH optimum at around 7.0. The enzyme exhibited a temperature optimum at around 30 degrees C. No temperature optimum was found for the cofactor. The enzyme was heat-stable and could oxidize manganese under anaerobic conditions. The enzyme system appears to be different from others so far described.
{"title":"A novel MN2+-oxidizing enzyme system in a freshwater bacterium.","authors":"J. Zindulis, H. Ehrlich","doi":"10.1002/JOBM.19830230715","DOIUrl":"https://doi.org/10.1002/JOBM.19830230715","url":null,"abstract":"Manganese oxidation by cell suspensions and cell extracts of a freshwater bacterium, designated strain FMn 1, was investigated. Manganese appeared to be oxidized in the periplasmic space. A conventional, membrane-bound-electron transport system was not utilized. An enzyme or enzyme complex and a cofactor, each of different molecular size, were located in different parts of the cell envelope. Results suggest that the cofactor reacts with manganese in the periplasmic space and that in the presence of oxygen it is reoxidized by the enzyme. The enzyme is probably loosely bound to the membrane. A combination of enzyme and cofactor in a crude preparation exhibited a pH optimum at around 7.0. The enzyme exhibited a temperature optimum at around 30 degrees C. No temperature optimum was found for the cofactor. The enzyme was heat-stable and could oxidize manganese under anaerobic conditions. The enzyme system appears to be different from others so far described.","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"21 1","pages":"457-65"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73905657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Effects of rice-field herbicide benthiocarb (S(4-chlorobenzyl)-N,N-diethyl thiolcarbamate) was studied on the nitrogen-fixing blue-green alga Nostoc linckia. The herbicide caused inhibition of growth and heterocyst formation, an increase in intensity of photoacoustic signals, and a four-fold reduction in oxygen evolution, but did not affect dark O2-uptake. The inhibition of growth and heterocyst formation was relieved by 500 micrograms/ml glucose. A Het-Nif- mutant of Nostoc muscorum failed to show an increase in reversion, frequency after treatment with 10 micrograms/ml benthiocarb for 1 hr.
{"title":"Inhibition of photosystem II of nitrogen-fixing blue-green alga Nostoc linckia by the rice-field herbicide benthiocarb.","authors":"R K Singh, B D Singh, H N Singh","doi":"10.1002/jobm.3630230708","DOIUrl":"https://doi.org/10.1002/jobm.3630230708","url":null,"abstract":"<p><p>Effects of rice-field herbicide benthiocarb (S(4-chlorobenzyl)-N,N-diethyl thiolcarbamate) was studied on the nitrogen-fixing blue-green alga Nostoc linckia. The herbicide caused inhibition of growth and heterocyst formation, an increase in intensity of photoacoustic signals, and a four-fold reduction in oxygen evolution, but did not affect dark O2-uptake. The inhibition of growth and heterocyst formation was relieved by 500 micrograms/ml glucose. A Het-Nif- mutant of Nostoc muscorum failed to show an increase in reversion, frequency after treatment with 10 micrograms/ml benthiocarb for 1 hr.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 7","pages":"435-41"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17475997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nalidixic acid induces segregation of auxotrophs from prototrophic hybrids of Candida albicans artifically produced by fusing complementing auxotrophic protoplasts. The auxotrophies recovered are limited to those introduced through the fusion process, and patterns of segregations for linked auxotrophic markers demonstrate the segregants are products mitotic crossing-over. Nalidixic acid does not induce auxotrophies of any sort in clinical isolates of C. albicans. These findings are contrary to a current hypothesis that natural strains of C. albicans are diploid and heterozygous for a variety of auxotrophic mutations.
{"title":"Recombinogenic activity of nalidixic acid for artificial hybrids but not for natural strains of Candida albicans: evidence for the monoploidy of natural strains.","authors":"A Sarachek","doi":"10.1002/jobm.3630230608","DOIUrl":"https://doi.org/10.1002/jobm.3630230608","url":null,"abstract":"<p><p>Nalidixic acid induces segregation of auxotrophs from prototrophic hybrids of Candida albicans artifically produced by fusing complementing auxotrophic protoplasts. The auxotrophies recovered are limited to those introduced through the fusion process, and patterns of segregations for linked auxotrophic markers demonstrate the segregants are products mitotic crossing-over. Nalidixic acid does not induce auxotrophies of any sort in clinical isolates of C. albicans. These findings are contrary to a current hypothesis that natural strains of C. albicans are diploid and heterozygous for a variety of auxotrophic mutations.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 6","pages":"385-91"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17416392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
More than 30 conjugative R plasmids between 60 and 70 Md in size were identified in wild type strains of Enterobacteriaceae isolated in German Democratic Republic, Bulgaria, Mongolia, Poland, Ethiopia, Iraque, and Soviet Union. They have been characterized by means of several genetic and molecular techniques as members of a new incompatibility group, termed IncZ. The properties of these plasmids including digestion pattern after EcoRI treatment demonstrate a phylogenetic relatedness.
{"title":"Characterization of conjugative plasmids belonging to a new incompatibility group (IncZ).","authors":"H Tschäpe, E Tietze","doi":"10.1002/jobm.3630230610","DOIUrl":"https://doi.org/10.1002/jobm.3630230610","url":null,"abstract":"<p><p>More than 30 conjugative R plasmids between 60 and 70 Md in size were identified in wild type strains of Enterobacteriaceae isolated in German Democratic Republic, Bulgaria, Mongolia, Poland, Ethiopia, Iraque, and Soviet Union. They have been characterized by means of several genetic and molecular techniques as members of a new incompatibility group, termed IncZ. The properties of these plasmids including digestion pattern after EcoRI treatment demonstrate a phylogenetic relatedness.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 6","pages":"393-401"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17416393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two newly isolated aniline-degrading bacterial strains were characterized with regard to their enzyme systems responsible for aniline catabolism. One of them identified as a Rhodococcus sp. metabolized aniline exclusively via the beta-ketoadipate pathway by means of inducible enzymes. The aniline-degrading enzyme system of the second isolate, presumably a pseudomonad, was shown to consist of an inducible aniline-converting enzyme and constitutive meta-pathway enzymes. Both isolates failed to metabolize monochlorinated anilines in the absence of additional carbon sources. To explain this the ring-cleaving enzymes of both isolates were examined for their substrate specificities. Furthermore, the effect of 4-chlorocatechol on the enzymes catalyzing aniline conversion and catechol oxygenation was investigated.
{"title":"Degradation of aniline and monochloroanilines by Rhodococcus sp. An 117 and a pseudomonad: a comparative study.","authors":"U Kaminski, D Janke, H Prauser, W Fritsche","doi":"10.1002/jobm.3630230405","DOIUrl":"https://doi.org/10.1002/jobm.3630230405","url":null,"abstract":"<p><p>Two newly isolated aniline-degrading bacterial strains were characterized with regard to their enzyme systems responsible for aniline catabolism. One of them identified as a Rhodococcus sp. metabolized aniline exclusively via the beta-ketoadipate pathway by means of inducible enzymes. The aniline-degrading enzyme system of the second isolate, presumably a pseudomonad, was shown to consist of an inducible aniline-converting enzyme and constitutive meta-pathway enzymes. Both isolates failed to metabolize monochlorinated anilines in the absence of additional carbon sources. To explain this the ring-cleaving enzymes of both isolates were examined for their substrate specificities. Furthermore, the effect of 4-chlorocatechol on the enzymes catalyzing aniline conversion and catechol oxygenation was investigated.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 4","pages":"235-46"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17668142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oxalic acid (as oxalate) was detected in four tubers commonly used for food in Nigeria-Dioscorea rotundata (White yam), Solanum tuberosum (Irish potato), Ipomoea batatas (Sweet potato), and Manihot esculenta (cassava). Whereas healthy I. batata had the highest oxalic acid content, healthy M. esculenta contained the lowest. When all tubers were artifically inoculated with four fungi-Penicillium oxalicum CURIE and THOM, Aspergillus niger VAN TIEGH, A. flavus and A. tamarii KITA, there was an increase in oxalate content/g of tuber tissue. The greatest amount of oxalate was produced by P. oxalicum in D. rotundata tuber. Consistently higher amounts of oxalate were produced by the four fungi in infected sweet potato tuber than in any other tuber and consistently lower amounts of oxalate were produced by the four fungi in Irish potato tuber. Differences in the carbohydrate type present in the tubers and in the biosynthesis pathway are thought to be responsible for variation in the production of oxalate in the different tubers by the four fungi used.
{"title":"Production of oxalic acid by some fungi infected tubers.","authors":"O Faboya, T Ikotun, O S Fatoki","doi":"10.1002/jobm.3630231003","DOIUrl":"https://doi.org/10.1002/jobm.3630231003","url":null,"abstract":"<p><p>Oxalic acid (as oxalate) was detected in four tubers commonly used for food in Nigeria-Dioscorea rotundata (White yam), Solanum tuberosum (Irish potato), Ipomoea batatas (Sweet potato), and Manihot esculenta (cassava). Whereas healthy I. batata had the highest oxalic acid content, healthy M. esculenta contained the lowest. When all tubers were artifically inoculated with four fungi-Penicillium oxalicum CURIE and THOM, Aspergillus niger VAN TIEGH, A. flavus and A. tamarii KITA, there was an increase in oxalate content/g of tuber tissue. The greatest amount of oxalate was produced by P. oxalicum in D. rotundata tuber. Consistently higher amounts of oxalate were produced by the four fungi in infected sweet potato tuber than in any other tuber and consistently lower amounts of oxalate were produced by the four fungi in Irish potato tuber. Differences in the carbohydrate type present in the tubers and in the biosynthesis pathway are thought to be responsible for variation in the production of oxalate in the different tubers by the four fungi used.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 10","pages":"621-4"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17725469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Growing L-form cells of Streptomyces hygroscopicus were shown to carry out 3-0-acylation and 14-C-hydroxylation of a macrolide glycoside suggesting that both types of bioconversion do neither require the intact cell wall nor the periplasmic space.
吸湿链霉菌生长的l型细胞可以对大环内酯糖苷进行3-0酰化和14- c -羟基化,这表明这两种类型的生物转化既不需要完整的细胞壁,也不需要细胞质周围空间。
{"title":"Bioconversions of a macrolide glycoside by growing L-form cells of Streptomyces hygroscopicus.","authors":"U Gräfe, J Gumpert","doi":"10.1002/jobm.3630230907","DOIUrl":"https://doi.org/10.1002/jobm.3630230907","url":null,"abstract":"<p><p>Growing L-form cells of Streptomyces hygroscopicus were shown to carry out 3-0-acylation and 14-C-hydroxylation of a macrolide glycoside suggesting that both types of bioconversion do neither require the intact cell wall nor the periplasmic space.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 9","pages":"565-9"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17725472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Temperature and pH significantly affect the growth and oospore production of the test fungi. The optimum temperature for mycelial production was nearly the same on both solid and liquid media. H-ion concentration has milder effect than temperature. The optimum temperature and pH value for oospore production agree remarkably with their respectives for growth.
{"title":"Effects of temperature and pH on growth and oospore production of three water-borne Pythium.","authors":"H M El-Sharouny","doi":"10.1002/jobm.3630230102","DOIUrl":"https://doi.org/10.1002/jobm.3630230102","url":null,"abstract":"<p><p>Temperature and pH significantly affect the growth and oospore production of the test fungi. The optimum temperature for mycelial production was nearly the same on both solid and liquid media. H-ion concentration has milder effect than temperature. The optimum temperature and pH value for oospore production agree remarkably with their respectives for growth.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 1","pages":"3-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17919888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}