首页 > 最新文献

Zeitschrift für Naturforschung C最新文献

英文 中文
Characterization of the cardiac glycoside and lipid profiles of Strophanthus kombé Oliv. seeds 茯苓心糖苷和脂质谱的研究。种子
Pub Date : 2016-03-01 DOI: 10.1515/znc-2015-0186
D. Knittel, P. Lorenz, U. Huber, F. Stintzing, Dietmar Kammerer
Abstract The seeds of Strophanthus kombé Oliv. are known to contain high levels of cardioactive compounds. However, the therapeutic use of Strophanthus in the treatment of cardiopathy requires more detailed knowledge of the compound profile to profit from the full potential of Strophanthus preparations. Therefore, the objective was to characterize the cardenolide profile and lipophilic constituents in S. kombé seeds using methods applicable in routine quality control. Freshly prepared S. kombé seed extracts were analyzed without previous sample clean-up using a novel HPLC-DAD-MSn method. In addition, seed oils were analyzed by GC-MS following derivatization of the lipids. More than 20 cardenolides were tentatively assigned in the seed extracts including strophanthidin, strophanthidol, periplogenin and strophanthidinic acid aglycones, carrying various saccharide moieties. The findings revealed the presence of eight novel cardenolides, which have not been described for S. kombé so far. The occurrence of strophanthidinic acid derivatives was verified by comparison with synthesized strophanthidinic acid-cymaropyranoside. GC-MS characterization of the oils mainly revealed the presence of fatty acids, especially oleic acid and linoleic acid, as well as phytosterols, the latter representing intermediates of cardenolide biosynthesis. In summary, these findings broaden our knowledge on the secondary metabolism of Strophanthus.
摘要:石竹属植物的种子。都含有高水平的心脏活性化合物。然而,Strophanthus在治疗心脏病方面的治疗用途需要更详细的化合物概况知识,以从Strophanthus制剂的全部潜力中获利。因此,目的是利用适用于常规质量控制的方法来表征红薯种子中的桃香内酯和亲脂性成分。采用新的HPLC-DAD-MSn方法对新鲜制备的红豆籽提取物进行分析,无需事先进行样品清理。此外,脂类衍生化后,采用气相色谱-质谱分析了种子油。经初步鉴定,其种子提取物中含有20余种桃核苷类,包括strophanthidin、strophanthidol、periplogenin和strophanthidacid gly锥体,携带不同的糖基。研究结果显示,存在8种新的桂枝内酯,到目前为止还没有在S. komb中描述过。通过与合成的strophantidic -cymaropyranoside进行比较,证实了strophantidic acid衍生物的存在。气相色谱-质谱分析结果显示,油酸和亚油酸是主要的脂肪酸成分,而植物甾醇是硬核内酯生物合成的中间体。总之,这些发现拓宽了我们对藤属植物次生代谢的认识。
{"title":"Characterization of the cardiac glycoside and lipid profiles of Strophanthus kombé Oliv. seeds","authors":"D. Knittel, P. Lorenz, U. Huber, F. Stintzing, Dietmar Kammerer","doi":"10.1515/znc-2015-0186","DOIUrl":"https://doi.org/10.1515/znc-2015-0186","url":null,"abstract":"Abstract The seeds of Strophanthus kombé Oliv. are known to contain high levels of cardioactive compounds. However, the therapeutic use of Strophanthus in the treatment of cardiopathy requires more detailed knowledge of the compound profile to profit from the full potential of Strophanthus preparations. Therefore, the objective was to characterize the cardenolide profile and lipophilic constituents in S. kombé seeds using methods applicable in routine quality control. Freshly prepared S. kombé seed extracts were analyzed without previous sample clean-up using a novel HPLC-DAD-MSn method. In addition, seed oils were analyzed by GC-MS following derivatization of the lipids. More than 20 cardenolides were tentatively assigned in the seed extracts including strophanthidin, strophanthidol, periplogenin and strophanthidinic acid aglycones, carrying various saccharide moieties. The findings revealed the presence of eight novel cardenolides, which have not been described for S. kombé so far. The occurrence of strophanthidinic acid derivatives was verified by comparison with synthesized strophanthidinic acid-cymaropyranoside. GC-MS characterization of the oils mainly revealed the presence of fatty acids, especially oleic acid and linoleic acid, as well as phytosterols, the latter representing intermediates of cardenolide biosynthesis. In summary, these findings broaden our knowledge on the secondary metabolism of Strophanthus.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"32 1","pages":"55 - 64"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84572752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Thonningiiflavanonol A and thonningiiflavanonol B, two novel flavonoids, and other constituents of Ficus thonningii Blume (Moraceae) 桑科桑榕中两种新型黄酮A、B及其他成分
Pub Date : 2016-03-01 DOI: 10.1515/znc-2015-0147
P. Ango, D. G. Kapche, G. Fotso, Christian D Fozing, Elizabeth M. O. Yeboah, Renameditswe Mapitse, I. Demirtaş, B. Ngadjui, S. Yeboah
Abstract A phytochemical study of Ficus thonningii has led to the isolation of two previously unreported compounds, thonningiiflavanonol A and thonningiiflavanonol B together with 16 known compounds: shuterin, naringenin, syringic acid, p-hydroxybenzoic acid, genistein, 5,7,3′,4′,5′-pentahydroxyflavanone, luteolin, methylparaben, aromadendrin, garbanzol, dihydroquercetin, 5,7,3′-trihydroxyflavanone, β-sitosterol, sitosterolglucoside, lupeol acetate, and taraxerol. Their structures were elucidated on the basis of spectroscopic data. The new compounds and extracts displayed potent antioxidant activity.
摘要:通过对无花果的植物化学研究,分离出了两种以前未报道的化合物:thonningiiflavanonol A和thonningiiflavanonol B,以及16种已知化合物:树黄素、柚皮素、丁香酸、对羟基苯甲酸、染料木素、5,7,3 '、4 '、5 ' -五羟基黄酮、木犀草素、对羟基羟基苯甲酸甲酯、芳香腺嘌呤、garbanzol、二氢槲皮素、5,7,3 ' -三羟基黄酮、β-谷甾醇、谷甾醇葡萄糖苷、lupepeol醋酸酯和taraxerol。根据光谱数据对它们的结构进行了分析。新化合物和提取物显示出较强的抗氧化活性。
{"title":"Thonningiiflavanonol A and thonningiiflavanonol B, two novel flavonoids, and other constituents of Ficus thonningii Blume (Moraceae)","authors":"P. Ango, D. G. Kapche, G. Fotso, Christian D Fozing, Elizabeth M. O. Yeboah, Renameditswe Mapitse, I. Demirtaş, B. Ngadjui, S. Yeboah","doi":"10.1515/znc-2015-0147","DOIUrl":"https://doi.org/10.1515/znc-2015-0147","url":null,"abstract":"Abstract A phytochemical study of Ficus thonningii has led to the isolation of two previously unreported compounds, thonningiiflavanonol A and thonningiiflavanonol B together with 16 known compounds: shuterin, naringenin, syringic acid, p-hydroxybenzoic acid, genistein, 5,7,3′,4′,5′-pentahydroxyflavanone, luteolin, methylparaben, aromadendrin, garbanzol, dihydroquercetin, 5,7,3′-trihydroxyflavanone, β-sitosterol, sitosterolglucoside, lupeol acetate, and taraxerol. Their structures were elucidated on the basis of spectroscopic data. The new compounds and extracts displayed potent antioxidant activity.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"18 1","pages":"65 - 71"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77501280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Some rape/canola seed oils: fatty acid composition and tocopherols 一些油菜/菜籽油:脂肪酸组成和生育酚
Pub Date : 2016-03-01 DOI: 10.1515/znc-2016-0003
B. Matthaus, M. Özcan, F. Al Juhaimi
Abstract Seed samples of some rape and canola cultivars were analysed for oil content, fatty acid and tocopherol profiles. Gas liquid chromotography and high performance liquid chromotography were used for fatty acid and tocopherol analysis, respectively. The oil contents of rape and canola seeds varied between 30.6% and 48.3% of the dry weight (p<0.05). The oil contents of rapeseeds were found to be high compared with canola seed oils. The main fatty acids in the oils are oleic (56.80–64.92%), linoleic (17.11–20.92%) and palmitic (4.18–5.01%) acids. A few types of tocopherols were found in rape and canola oils in various amounts: α-tocopherol, γ-tocopherol, δ-tocopherol, β-tocopherol and α-tocotrienol. The major tocopherol in the seed oils of rape and canola cultivars were α-tocopherol (13.22–40.01%) and γ-tocopherol (33.64–51.53%) accompanied by α-T3 (0.0–1.34%) and δ-tocopherol (0.25–1.86%) (p<0.05). As a result, the present study shows that oil, fatty acid and tocopherol contents differ significantly among the cultivars.
摘要对部分油菜和油菜品种的种子样品进行了含油量、脂肪酸和生育酚的分析。脂肪酸分析采用气相液相色谱法,生育酚分析采用高效液相色谱法。油菜和菜籽含油量占干重的比例在30.6% ~ 48.3%之间(p<0.05)。菜籽油的含油量比菜籽油高。油中的主要脂肪酸为油酸(56.80 ~ 64.92%)、亚油酸(17.11 ~ 20.92%)和棕榈酸(4.18 ~ 5.01%)。油菜和菜籽油中含有α-生育酚、γ-生育酚、δ-生育酚、β-生育酚和α-生育三烯酚。油菜和菜籽油中主要的生育酚为α-生育酚(13.22 ~ 40.01%)和γ-生育酚(33.64 ~ 51.53%),其次为α-T3(0.0 ~ 1.34%)和δ-生育酚(0.25 ~ 1.86%)(p<0.05)。结果表明,不同品种间的油分、脂肪酸和生育酚含量差异显著。
{"title":"Some rape/canola seed oils: fatty acid composition and tocopherols","authors":"B. Matthaus, M. Özcan, F. Al Juhaimi","doi":"10.1515/znc-2016-0003","DOIUrl":"https://doi.org/10.1515/znc-2016-0003","url":null,"abstract":"Abstract Seed samples of some rape and canola cultivars were analysed for oil content, fatty acid and tocopherol profiles. Gas liquid chromotography and high performance liquid chromotography were used for fatty acid and tocopherol analysis, respectively. The oil contents of rape and canola seeds varied between 30.6% and 48.3% of the dry weight (p<0.05). The oil contents of rapeseeds were found to be high compared with canola seed oils. The main fatty acids in the oils are oleic (56.80–64.92%), linoleic (17.11–20.92%) and palmitic (4.18–5.01%) acids. A few types of tocopherols were found in rape and canola oils in various amounts: α-tocopherol, γ-tocopherol, δ-tocopherol, β-tocopherol and α-tocotrienol. The major tocopherol in the seed oils of rape and canola cultivars were α-tocopherol (13.22–40.01%) and γ-tocopherol (33.64–51.53%) accompanied by α-T3 (0.0–1.34%) and δ-tocopherol (0.25–1.86%) (p<0.05). As a result, the present study shows that oil, fatty acid and tocopherol contents differ significantly among the cultivars.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"39 1","pages":"73 - 77"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82455650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 43
Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines 大螺旋藻和黑木的化学成分及其对癌细胞的抗增殖作用
Pub Date : 2016-03-01 DOI: 10.1515/znc-2016-0010
R. Erenler, Köksal Pabuçcu, A. S. Yaglıoglu, I. Demirtaş, Fatih Gul
Abstract In this study, the effect of Mougeotia nummuloides and Spirulina major on Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigated in vitro. The antiproliferative effect of the methanol extract of M. nummuloides and S. major compared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. Both M. nummuloides and S. major extracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50 and IC75 values. The M. nummuloides extract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. The S. major extract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in the M. nummuloides extract where as in the S. major extract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.
摘要本实验以非洲绿猴肾Vero细胞、大鼠脑肿瘤细胞C6细胞和人子宫癌细胞HeLa细胞为实验对象,研究了木菇和大螺旋藻对小鼠肾Vero细胞、子宫癌细胞HeLa细胞的体外作用。采用BrdU细胞增殖酶联免疫吸附试验(BrdU Cell Proliferation ELISA),比较不同浓度下nummuloides和S. major甲醇提取物与5-氟尿嘧啶(5-FU)和顺铂的抗增殖作用。木犀草提取物对Vero、HeLa和C6癌细胞的增殖均有显著抑制作用(IC50和IC75)。在高浓度下,该提取物对Vero和C6细胞的活性高于5-FU和顺铂。在500 μg/mL浓度下,菝葜提取物对Vero细胞的抗增殖活性优于标准提取物。经硅烷化处理后,采用气相色谱-质谱法测定甲醇提取物的化合物。海藻糖、单硬脂素和1-单棕榈脂素被检测为主要产物;主要成分为单硬脂素、1-单棕榈醇和己醇。
{"title":"Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines","authors":"R. Erenler, Köksal Pabuçcu, A. S. Yaglıoglu, I. Demirtaş, Fatih Gul","doi":"10.1515/znc-2016-0010","DOIUrl":"https://doi.org/10.1515/znc-2016-0010","url":null,"abstract":"Abstract In this study, the effect of Mougeotia nummuloides and Spirulina major on Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigated in vitro. The antiproliferative effect of the methanol extract of M. nummuloides and S. major compared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. Both M. nummuloides and S. major extracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50 and IC75 values. The M. nummuloides extract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. The S. major extract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in the M. nummuloides extract where as in the S. major extract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"27 1","pages":"87 - 92"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89467384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
The physico-chemical properties of some citrus seeds and seed oils 柑桔类种子及其油的理化性质
Pub Date : 2016-03-01 DOI: 10.1515/znc-2016-0004
F. Juhaimi, B. Matthäus, M. Özcan, K. Ghafoor
Abstract The chemical properties, mineral contents, fatty acid and tocopherol contents of seed and seed oils of some citrus genus provided from several locations in Turkey and Saudi Arabia were determined. While Ca contents of seeds were between 5018 mg/kg (Kütdiken lemon) and 7619 mg/kg (kinnow mandarin), K contents of seeds varied between 7007 mg/kg (Orlando orange) and 10334 mg/kg (kinnow mandarin). Glucose and fructose contents of citrus seed samples varied between 3.75 g/kg and 5.75 g/kg, and 4.09 g/kg and 6.03 g/kg. Palmitic, oleic and linoleic acids were established as dominant fatty acids. Palmitic, oleic and linoleic acid contents of citrus seed oils varied between 19.6% (Kütdiken lemon) and 26.2% (pineapple orange), 21.3% (kinnow mandarin) and 31.4% (Kütdiken lemon) and 32.3% (Kütdiken lemon) and 43.7% (kinnow mandarin), respectively. The total amount of tocopherols of Turkish citrus oil varied between 0.5 mg/100 g (Fremont mandarin) and 18.8 mg/100 g (bitter orange).
摘要对土耳其和沙特阿拉伯几种柑橘属植物种子和种子油的化学性质、矿物质含量、脂肪酸和生育酚含量进行了测定。种子中Ca含量在5018 ~ 7619 mg/kg (k迪肯柠檬)之间,K含量在7007 ~ 10334 mg/kg(金诺柑橘)之间。柑橘种子样品葡萄糖和果糖含量分别为3.75 g/kg ~ 5.75 g/kg和4.09 g/kg ~ 6.03 g/kg。棕榈酸、油酸和亚油酸被确定为主要脂肪酸。柑橘籽油的棕榈酸、油酸和亚油酸含量分别为19.6% (k地肯柠檬)和26.2%(菠萝橙),21.3% (k地肯柠檬)和31.4% (kinnow柑),32.3% (k地肯柠檬)和43.7% (kinnow柑)。土耳其柑橘油中生育酚的含量在0.5 mg/ 100g(佛利蒙柑)和18.8 mg/ 100g(苦橙)之间变化。
{"title":"The physico-chemical properties of some citrus seeds and seed oils","authors":"F. Juhaimi, B. Matthäus, M. Özcan, K. Ghafoor","doi":"10.1515/znc-2016-0004","DOIUrl":"https://doi.org/10.1515/znc-2016-0004","url":null,"abstract":"Abstract The chemical properties, mineral contents, fatty acid and tocopherol contents of seed and seed oils of some citrus genus provided from several locations in Turkey and Saudi Arabia were determined. While Ca contents of seeds were between 5018 mg/kg (Kütdiken lemon) and 7619 mg/kg (kinnow mandarin), K contents of seeds varied between 7007 mg/kg (Orlando orange) and 10334 mg/kg (kinnow mandarin). Glucose and fructose contents of citrus seed samples varied between 3.75 g/kg and 5.75 g/kg, and 4.09 g/kg and 6.03 g/kg. Palmitic, oleic and linoleic acids were established as dominant fatty acids. Palmitic, oleic and linoleic acid contents of citrus seed oils varied between 19.6% (Kütdiken lemon) and 26.2% (pineapple orange), 21.3% (kinnow mandarin) and 31.4% (Kütdiken lemon) and 32.3% (Kütdiken lemon) and 43.7% (kinnow mandarin), respectively. The total amount of tocopherols of Turkish citrus oil varied between 0.5 mg/100 g (Fremont mandarin) and 18.8 mg/100 g (bitter orange).","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"51 1","pages":"79 - 85"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88165747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Acute toxicity, antiedematogenic activity, and chemical constituents of Palicourea rigida Kunth 硬毛孢的急性毒性、抗水肿活性及化学成分研究
Pub Date : 2016-03-01 DOI: 10.1515/znc-2015-0036
V. G. Alves, Elisa A. da Rosa, L. L. D. de Arruda, B. Rocha, C. B. Bersani Amado, S. Santin, A. Pomini, C. C. da Silva
Abstract The phytochemical study of the leaves, roots, and flowers of Palicourea rigida led to the isolation of the triterpenes betulinic acid (1) and lupeol (2), the diterpene phytol (3), and the iridoid glycosides sweroside (4) and secoxyloganin (5). These compounds were identified using NMR 1H and 13C and comparing the spectra with published data. We studied the antiedematogenic activity of crude extracts from the organs, and of different fractions, in mice and found that the n-hexane fraction of the leaf extract significantly inhibited the ear edema resulting from croton oil administration. The crude extract from leaves was not acutely toxic to the mice.
摘要:通过对palicourrea rigida的叶、根和花的植物化学研究,分离得到三萜白桦酸(1)和芦皮醇(2),二萜叶绿醇(3),环烯醚萜类苷甜菊苷(4)和刺草苷(5)。利用NMR 1H和13C对这些化合物进行了鉴定,并与已发表的数据进行了比较。我们研究了不同部位的器官粗提物对小鼠的抗水肿活性,发现叶提取物的正己烷部分显著抑制了巴豆油引起的耳部水肿。叶片粗提物对小鼠没有急性毒性。
{"title":"Acute toxicity, antiedematogenic activity, and chemical constituents of Palicourea rigida Kunth","authors":"V. G. Alves, Elisa A. da Rosa, L. L. D. de Arruda, B. Rocha, C. B. Bersani Amado, S. Santin, A. Pomini, C. C. da Silva","doi":"10.1515/znc-2015-0036","DOIUrl":"https://doi.org/10.1515/znc-2015-0036","url":null,"abstract":"Abstract The phytochemical study of the leaves, roots, and flowers of Palicourea rigida led to the isolation of the triterpenes betulinic acid (1) and lupeol (2), the diterpene phytol (3), and the iridoid glycosides sweroside (4) and secoxyloganin (5). These compounds were identified using NMR 1H and 13C and comparing the spectra with published data. We studied the antiedematogenic activity of crude extracts from the organs, and of different fractions, in mice and found that the n-hexane fraction of the leaf extract significantly inhibited the ear edema resulting from croton oil administration. The crude extract from leaves was not acutely toxic to the mice.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"59 1","pages":"39 - 43"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73903525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Fatty acid composition of achenes of Cirsium taxa (Asteraceae, Carduoideae) from Turkey 土耳其卷叶卷叶卷叶类群瘦果的脂肪酸组成
Pub Date : 2016-03-01 DOI: 10.1515/znc-2015-0128
M. Ozcan, F. Ayaz, Y. Ozogul, R. Glew, F. Ozogul
Abstract The fatty acid compositions and total oil contents in achenes of 22 Cirsium taxa collected from different natural habitats in Turkey were investigated. The range of total fat in the taxa varied from 1.7% to 13.3%. The taxa contained palmitic (C16:0), stearic (C18:0), oleic (C18:1n-9), linoleic (C18:2n-6), and α-linolenic (C18:3n-3) acid. Polyunsaturated fatty acids had the highest level, ranging from 52.1% to 75.2% (C18:2n-6) and 0.5%–17.3% (C18:3n-3). Total saturated (6.9%–17.4%), monounsaturated (11.7%–28.9%), and polyunsaturated (57.4%–79.9%) fatty acids varied substantially, whereas total unsaturated fatty acids ranged from 70.6% to 91.3%. Considerable variation was observed (P<0.05) within each taxon for these fatty acids, especially α-linolenic acid (C18:3n-3). Fatty acid compositions of the 22 taxa were compared by cluster analysis (UPGMA) and principle component analysis (PCA) based on six major fatty acids. This is the first report on the lipid content and fatty acid composition of achenes of Cirsium taxa growing in different areas of Turkey. Fatty acid profiles, relative proportions and levels of fatty acids can be used as additional biochemical markers in the taxonomy of Cirsium.
摘要对采自土耳其不同自然生境的22个卷叶草类群瘦果的脂肪酸组成和总油含量进行了研究。总脂肪含量在1.7% ~ 13.3%之间。该分类群含有棕榈酸(C16:0)、硬脂酸(C18:0)、油酸(C18:1n-9)、亚油酸(C18:2n-6)和α-亚麻酸(C18:3n-3)。多不饱和脂肪酸含量最高,分别为52.1% ~ 75.2% (C18:2n-6)和0.5% ~ 17.3% (C18:3n-3)。饱和脂肪酸总量(6.9%-17.4%)、单不饱和脂肪酸总量(11.7%-28.9%)和多不饱和脂肪酸总量(57.4%-79.9%)变化很大,而不饱和脂肪酸总量在70.6% - 91.3%之间。这些脂肪酸在各分类群间差异较大(P<0.05),其中α-亚麻酸(C18:3n-3)差异最大。采用聚类分析(UPGMA)和基于6种主要脂肪酸的主成分分析(PCA)对22个类群的脂肪酸组成进行比较。本文首次报道了生长在土耳其不同地区的卷叶草类群瘦果的脂质含量和脂肪酸组成。脂肪酸谱、脂肪酸的相对比例和水平可以作为茜草分类学的附加生化标记。
{"title":"Fatty acid composition of achenes of Cirsium taxa (Asteraceae, Carduoideae) from Turkey","authors":"M. Ozcan, F. Ayaz, Y. Ozogul, R. Glew, F. Ozogul","doi":"10.1515/znc-2015-0128","DOIUrl":"https://doi.org/10.1515/znc-2015-0128","url":null,"abstract":"Abstract The fatty acid compositions and total oil contents in achenes of 22 Cirsium taxa collected from different natural habitats in Turkey were investigated. The range of total fat in the taxa varied from 1.7% to 13.3%. The taxa contained palmitic (C16:0), stearic (C18:0), oleic (C18:1n-9), linoleic (C18:2n-6), and α-linolenic (C18:3n-3) acid. Polyunsaturated fatty acids had the highest level, ranging from 52.1% to 75.2% (C18:2n-6) and 0.5%–17.3% (C18:3n-3). Total saturated (6.9%–17.4%), monounsaturated (11.7%–28.9%), and polyunsaturated (57.4%–79.9%) fatty acids varied substantially, whereas total unsaturated fatty acids ranged from 70.6% to 91.3%. Considerable variation was observed (P<0.05) within each taxon for these fatty acids, especially α-linolenic acid (C18:3n-3). Fatty acid compositions of the 22 taxa were compared by cluster analysis (UPGMA) and principle component analysis (PCA) based on six major fatty acids. This is the first report on the lipid content and fatty acid composition of achenes of Cirsium taxa growing in different areas of Turkey. Fatty acid profiles, relative proportions and levels of fatty acids can be used as additional biochemical markers in the taxonomy of Cirsium.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"2010 1","pages":"45 - 54"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86279892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria 保加利亚富马菌生物碱谱及乙酰胆碱酯酶抑制活性
Pub Date : 2016-01-01 DOI: 10.1515/znc-2014-4179
R. Vrancheva, Ivan Georgiev Ivanov, I. Aneva, I. Dincheva, I. Badjakov, A. Pavlov
Abstract GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC50 0.13 ± 0.01 mg extract/mL).
摘要采用气相色谱-质谱法对保加利亚5种天然富马属(F. officinalis、F. thuretii、F. kralikii、F. rostellata和F. schrammii)的生物碱谱进行了分析,并对生物碱提取物的乙酰胆碱酯酶抑制活性进行了分析。共鉴定出14种异喹啉类生物碱,主要为原托碱、隐托碱、sinactine、parfumine、fumariline、fumarophycine和fumaritine。HPLC测定的原托氨酸含量在210.6±8.8 μg/g DW (F. schrammii)和334.5±7.1 μg/g DW之间变化。(f . rostellata)。所有生物碱提取物均能显著抑制乙酰胆碱酯酶活性,其中克拉氏弧菌的IC50值最高(0.13±0.01 mg提取物/mL)。
{"title":"Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria","authors":"R. Vrancheva, Ivan Georgiev Ivanov, I. Aneva, I. Dincheva, I. Badjakov, A. Pavlov","doi":"10.1515/znc-2014-4179","DOIUrl":"https://doi.org/10.1515/znc-2014-4179","url":null,"abstract":"Abstract GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC50 0.13 ± 0.01 mg extract/mL).","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"33 1","pages":"14 - 9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76163337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Unguisin F, a new cyclic peptide from the endophytic fungus Mucor irregularis 从内生真菌不规则毛霉中提取的一种新的环状肽
Pub Date : 2016-01-01 DOI: 10.1515/znc-2015-0137
S. H. Akoné, G. Daletos, Wenhan Lin, P. Proksch
Abstract The new cyclic heptapeptide unguisin F (1) and the known congener unguisin E (2), were obtained from the endophytic fungus Mucor irregularis, isolated from the medicinal plant Moringa stenopetala, collected in Cameroon. The structure of the new compound was unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configuration of the amino acid residues of 1 and 2 was determined using Marfey’s analysis. Compounds 1 and 2 were evaluated for their antibacterial and antifungal potential, but failed to display significant activities.
摘要从喀麦隆药用植物辣木(Moringa stenopetala)内生真菌Mucor irregularis中分离得到新的环七肽unguisin F(1)和已知的同源物unguisin E(2)。新化合物的结构是在一维和二维核磁共振波谱以及高分辨率质谱的基础上明确确定的。利用Marfey分析确定了1和2的氨基酸残基的绝对构型。化合物1和2具有抗菌和抗真菌活性,但未显示出显著活性。
{"title":"Unguisin F, a new cyclic peptide from the endophytic fungus Mucor irregularis","authors":"S. H. Akoné, G. Daletos, Wenhan Lin, P. Proksch","doi":"10.1515/znc-2015-0137","DOIUrl":"https://doi.org/10.1515/znc-2015-0137","url":null,"abstract":"Abstract The new cyclic heptapeptide unguisin F (1) and the known congener unguisin E (2), were obtained from the endophytic fungus Mucor irregularis, isolated from the medicinal plant Moringa stenopetala, collected in Cameroon. The structure of the new compound was unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configuration of the amino acid residues of 1 and 2 was determined using Marfey’s analysis. Compounds 1 and 2 were evaluated for their antibacterial and antifungal potential, but failed to display significant activities.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"81 1","pages":"15 - 19"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79956961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Structural and evolutionary relationships among RuBisCOs inferred from their large and small subunits RuBisCOs之间的结构和进化关系从它们的大小亚基推断出来
Pub Date : 2016-01-01 DOI: 10.1515/znc-2016-0014
F. Xiang, Yuanping Fang, Jun Xiang
Abstract Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key enzyme to assimilate CO2 into the biosphere. The nonredundant structural data sets for three RuBisCO domain superfamilies, i.e. large subunit C-terminal domain (LSC), large subunit N-terminal domain (LSN) and small subunit domain (SS), were selected using QR factorization based on the structural alignment with QH as the similarity measure. The structural phylogenies were then constructed to investigate a possible functional significance of the evolutionary diversification. The LSC could have occurred in both bacteria and archaea, and has evolved towards increased complexity in both bacteria and eukaryotes with a 4-helix–2-helix–2-helix bundle being extended into a 5-helix–3-helix–3-helix one at the LSC carboxyl-terminus. The structural variations of LSN could have originated not only in bacteria with a short coil, but also in eukaryotes with a long one. Meanwhile, the SS dendrogram can be contributed to the structural variations at the βA–βB-loop region. All the structural variations observed in the coil regions have influence on catalytic performance or CO2/O2 selectivities of RuBisCOs from different species. Such findings provide insights on RuBisCO improvements.
核酮糖1,5-二磷酸羧化酶/加氧酶(RuBisCO)是生物圈吸收二氧化碳的关键酶。采用基于结构比对的QR分解方法,以QH为相似性度量,选取RuBisCO结构域超家族(大亚基c端域(LSC)、大亚基n端域(LSN)和小亚基域(SS)的非冗余结构数据集。然后构建了结构系统发育,以研究进化多样化的可能功能意义。LSC可能发生在细菌和古细菌中,并且在细菌和真核生物中进化得越来越复杂,在LSC羧基端,4-螺旋- 2-螺旋- 2-螺旋束被扩展成5-螺旋- 3-螺旋束。LSN的结构变异不仅可能起源于具有短线圈的细菌,也可能起源于具有长线圈的真核生物。同时,βA - β b环区域的结构变化也导致了SS树突图的出现。在线圈区域观察到的所有结构变化都对不同物种的RuBisCOs的催化性能或CO2/O2选择性产生影响。这些发现为RuBisCO的改进提供了见解。
{"title":"Structural and evolutionary relationships among RuBisCOs inferred from their large and small subunits","authors":"F. Xiang, Yuanping Fang, Jun Xiang","doi":"10.1515/znc-2016-0014","DOIUrl":"https://doi.org/10.1515/znc-2016-0014","url":null,"abstract":"Abstract Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key enzyme to assimilate CO2 into the biosphere. The nonredundant structural data sets for three RuBisCO domain superfamilies, i.e. large subunit C-terminal domain (LSC), large subunit N-terminal domain (LSN) and small subunit domain (SS), were selected using QR factorization based on the structural alignment with QH as the similarity measure. The structural phylogenies were then constructed to investigate a possible functional significance of the evolutionary diversification. The LSC could have occurred in both bacteria and archaea, and has evolved towards increased complexity in both bacteria and eukaryotes with a 4-helix–2-helix–2-helix bundle being extended into a 5-helix–3-helix–3-helix one at the LSC carboxyl-terminus. The structural variations of LSN could have originated not only in bacteria with a short coil, but also in eukaryotes with a long one. Meanwhile, the SS dendrogram can be contributed to the structural variations at the βA–βB-loop region. All the structural variations observed in the coil regions have influence on catalytic performance or CO2/O2 selectivities of RuBisCOs from different species. Such findings provide insights on RuBisCO improvements.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"28 1","pages":"181 - 189"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79030965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Zeitschrift für Naturforschung C
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1