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A new species of Gyrodactylus (Monogenea: Monopisthocotylea) on tadpoles of Rana catesbeiana from California, U.S.A. 美国加利福尼亚中华绒螯虾蝌蚪上的一新种(单属:单子叶目)
Pub Date : 1993-07-01 DOI: 10.2307/3226681
D. Wootton, K. Ryan, R. Demaree, Richard L Critchfield
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引用次数: 4
Histopathology in a captive yarrow's spiny lizard, Sceloporus jarrovii (Phrynosomatidae), attributed to the mite Hirstiella sp. (Pterygosomatidae) 圈养的雅罗刺蜥(刺蜥科)的组织病理学研究,归属于螨Hirstiella sp.(翼虫科)
Pub Date : 1993-07-01 DOI: 10.2307/3226682
S. Goldberg, H. J. Holshuh
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引用次数: 5
Two New Species of Strelkovimermis (Nematoda: Mermithidae) as Imagicidal Parasites of Chironomids (Diptera: Chironomidae) in Lake Itasca, Minnesota, U.S.A.
Pub Date : 1993-04-01 DOI: 10.2307/3226825
Arthur A. Johnson, M. Kleve
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引用次数: 3
Trophomermis itascensis n. gen., n. sp. (Nematoda: Mermithidae), an imagicidal parasite of Chironomids in Lake Itasca, Minnesota, U.S.A. 美国明尼苏达州伊塔斯卡湖水蛾类的一种杀象寄生虫——水蛾营养小蛾(线虫纲:水蛾科)
Pub Date : 1993-04-01 DOI: 10.2307/3226824
Arthur A. Johnson, M. Kleve
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引用次数: 2
A new species of Hypsibius (Phylum Tardigrada) from Roan Mountain, Tennessee, U.S.A. 标题美国田纳西州罗安山缓步虫门一新种
Pub Date : 1993-04-01 DOI: 10.2307/3226827
D. Nelson, Karen L. McGlothlin
A new tardigrade species, Hypsibius roanensis, is described from epiphytic mosses and lichens on beech trees on Roan Mountain, Tennessee. It differs from other species of the genus by the presence of a sculptured cuticle with irregular tubercles that increase in size caudally. The buccopharyngeal apparatus consists of a narrow buccal tube, well-developed apophyses, and a spherical pharynx with two granular macroplacoids. Roan Mountain is the highest cross range between the Unaka Mountains and the Blue Ridge Mountains in the Southern Appalachians. The north-facing slope of the mountain is in Carter County, Tennessee; the south-facing slope is in Mitchell County, North Carolina. Tardigrades were first recorded from Roan Mountain by Riggin (1962), who found three species of Macrobiotus at 1,890 m in Tennessee. Maucci (1987) reported 10 species in five genera (Macrobiotus, Diphascon, Minibiotus, Itaquascon, and Milnesium) from mosses collected in the spruce-fir forest of Roan Mountain above 1,829 m. The most extensive study of tardigrades on Roan Mountain was by Nelson (1973, 1975), who examined the ecological distribution of 21 species from epiphytic mosses on beech trees at approximately 1,219 m, 1,524 m, and 1,654 m on both the northand southfacing slopes. Nelson found nine specimens of a new species of Hypsibius (designated Hypsibius "a" in Nelson, 1975). However, multiple specimens from a sample were mounted per slide in a non-permanent medium (Turtox CMC9), and the slides were unsuitable for identification after 10 years. McGlothlin (1990) collected again at two of Nelson's sites to determine the long-term stability of tardigrade populations and rediscovered the new species of Hypsibius. The new species is described here. MATERIALS AND METHODS On 8 September 1988, epiphytes (primarily mosses) were collected from beech trees at approximately 1,524 m and 1,654 m on the north-facing slope of Roan Mountain, Carter County, Tennessee (Nelson's stations 5N and 6N). Fifty-six samples were collected, 27 from the 5N station and 29 from 6N. Each sample was placed in a stoppered funnel and soaked in tap water for 24 h to allow the animals to become active and then go into anoxybiosis. The epiphyte was removed and squeezed over the funnel to recover the maximal number of tardigrades. The contents of the funnel were drained into a beaker, and the top layer of water was decanted. Boiling water was added to the beaker to kill the tardigrades. Then the contents of the beaker were rinsed through two sieves, TRANS. AM. MICROSC. SOC., 112(2): 140-144. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.127 on Tue, 28 Jun 2016 06:58:43 UTC All use subject to http://about.jstor.org/terms VOL. 112, NO. 2, APRIL 1993 no. 18 mesh (1 mm) and no. 325 mesh (0.045 mm), simultaneously. The material remaining on the no. 325 sieve was washed into a jar with 95% ethanol. Later, the contents of the jars were examined in a
在美国田纳西州罗安山山毛榉上的附生苔藓和地衣中发现了一种缓步动物新种——罗安山毛榉。它不同于其他种类的属的存在与不规则的结节,在尾端增加大小的雕刻角质层。咽咽器由狭窄的咽管、发育良好的咽突和球形咽及两个颗粒状的大咽片组成。罗安山是阿巴拉契亚山脉南部乌纳卡山脉和蓝岭山脉之间最高的交叉山脉。山的北坡在田纳西州的卡特县;朝南的斜坡位于北卡罗来纳州的米切尔县。缓步动物最早是由Riggin(1962)在Roan山记录的,他在田纳西州1890米的地方发现了3种Macrobiotus。Maucci(1987)报道了在海拔1829米以上的罗安山云杉林中采集的藓类5属(Macrobiotus, Diphascon, Minibiotus, Itaquascon, Milnesium) 10种。对Roan山缓步动物最广泛的研究是由Nelson(1973, 1975)进行的,他研究了21种附生苔藓在山毛榉树上的生态分布,分布高度分别为1219米、1524米和1654米,分别位于北坡和南坡。纳尔逊发现了9个新物种Hypsibius的标本(Nelson在1975年命名为Hypsibius“a”)。然而,在非永久介质(Turtox CMC9)中,每张载玻片都装载了多个样品,并且载玻片在10年后不适合鉴定。McGlothlin(1990)再次在Nelson的两个地点收集数据,以确定缓步动物种群的长期稳定性,并重新发现了新物种Hypsibius。这里描述了新物种。材料与方法1988年9月8日,在田纳西州卡特县Roan山北坡约1524 m和1654 m处(Nelson站5N和6N)采集了山毛榉的附生植物(主要是苔藓)。共采集56份样品,其中5N站27份,6N站29份。每个样本被放置在一个带塞的漏斗中,在自来水中浸泡24小时,使动物变得活跃,然后进入缺氧状态。将附生菌移出并在漏斗上挤压,以恢复最大数量的缓步动物。漏斗的内容物被排到烧杯中,最上层的水被倒出。烧杯中加入沸水以杀死缓步动物。然后,烧杯里的东西通过两个筛子(TRANS)进行冲洗。点。MICROSC。SOC。浙江农业学报,12(2):140-144。1993. ? 版权所有,1993年,美国显微学会,Inc。此内容于2016年6月28日星期二06:58:43 UTC从157.55.39.127下载。1993年4月2日18目(1毫米),无。325目(0.045毫米),同时。残留在上面的材料。325筛子用95%乙醇洗入罐中。随后,在25倍暗场立体显微镜下的格子培养皿中检查罐子中的内容物。用欧文环将缓步动物转移到含有95%乙醇的小瓶中。每个样本最多安装35个缓步动物。每个标本用Irwin loop取出,放在Hoyer的安装介质中,放在显微镜载玻片上,用盖盖密封。在介质完全干燥后,用环氧涂料将盖盖圈起来。采用油浸干涉相衬复合显微镜进行鉴定。用校准过的眼测微计进行测量。图纸是用拔管绘制的,照片是用柯达T Max?100年的电影。扫描电子显微照片来自尼尔森(1973)对罗安山缓步动物的原始研究。
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引用次数: 13
Glochidial host of Alasmidonta atropurpurea (Bivalvia: Unionoidea, Unionidae)
Pub Date : 1993-04-01 DOI: 10.2307/3226828
M. E. Gordon, J. Layzer
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引用次数: 7
Adhesion Seams in the Tiedemann's Diverticula of the Starfish Henricia sanguinolenta 血海星Tiedemann憩室的粘连缝
Pub Date : 1993-04-01 DOI: 10.2307/3226830
J. C. Ferguson, C. Walker
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引用次数: 1
Lectin-binding differences on hemocytes of two geographic strains of the American oyster, Crassostrea virginica 美洲牡蛎两个地理品系凝集素结合在血细胞上的差异
Pub Date : 1993-04-01 DOI: 10.2307/3226829
T. Cheng, W. Dougherty, V. Burrell
The saccharidal constituents of the surfaces of hemocytes of the American oyster, Crassostrea virginica, from Apalachicola Bay, Florida, and Galveston Bay, Texas, were determined by employing eight lectins with known sugar specificities (Con A, Tetragonolobus purpureas, Limulus polyphemus, Dilichos biflorus, Sambucas nigra, Glycine max, Triticum vulgaris, and Lathyrus odoratus). Known inhibiting sugar residues were used to inhibit clumping of lectin-treated oyster hemocytes. As a result, the following saccharides were demonstrated to occur on oyster hemocytes: N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, methyl-a-D-mannopyranoside, D(+)-glucose, sucrose, D(+)mannose, a-methyl-D-galactoside, -D(-)-fructose, L(-)-fucose, N-acetylneuraminic acid, and D(+)-galactose. In addition, D-glucuronic acid occurred only on oyster cells from Apalachicola Bay and an unidentified sugar that binds to the L. odoratus lectin that is neither D(+)-glucose nor D(+)-mannose, the usual inhibiting sugars, occurs on hemocytes from both Apalachicola and Galveston Bays. Quantitative and qualitative differences in the saccharidal constituents on the surfaces of hemocytes from oysters from the two collecting sites are attributed to strain differences. Naturally occurring lectins in invertebrates have been repeatedly proposed, among other functions, to play a role in the recognition of self from nonself (Anderson & Good, 1976; Cheng, 1984; Cheng et al., 1984; Renwrantz, 1981). These proteins or glycoproteins include binding sites that recognize specific sugar residues. In order to identify the carbohydrates, which are the primary determinants of cellular identity of many cells (Gaveriaux & Loor, 1987), and by so doing increase our understanding of lectin-mediated attachment or nonattachment of biotic invaders, we have tested the effects of selected lectins with known saccharidal specificities on hemocytes of the American oyster, Crassostrea virginica (Gmelin). MATERIALS AND METHODS Oysters. The oysters employed in this study originated from two sources: Apalachicola Bay, Florida, and Galveston Bay, Texas, U.S.A. All were harvested during the last week in February through the second week in April 1992. All oysters were held in the laboratory at 3?C in 15% seawater until 1 h prior to bleeding. None was held for more than three days. i The outstanding technical assistance by Ms. Janet M. Barto is gratefully acknowledged. This research was supported by a grant (NA16FLO408-01) from the National Marine Fisheries Service, U.S. Department of Commerce. TRANS. AM. MICROSC. Soc., 112(2): 151-157. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 207.46.13.124 on Wed, 22 Jun 2016 05:21:35 UTC All use subject to http://about.jstor.org/terms TRANS. AM. MICROSC. SOC.
采用8种已知糖特异性的凝集素(Con A、Tetragonolobus purpureas、limus polyphemus、Dilichos biflorus、Sambucas nigra、Glycine max、Triticum vulgaris和Lathyrus odoratus)测定了产自佛罗里达州Apalachicola湾和德克萨斯州加尔维斯顿湾的美洲牡蛎(Crassostrea virginica)血红细胞表面的糖性成分。已知的抑制糖残基被用来抑制凝集素处理的牡蛎血细胞的结块。结果,以下糖类被证明发生在牡蛎血细胞上:n -乙酰-D-葡萄糖胺、n -乙酰-D-半乳糖胺、甲基-a-D-甘露糖、D(+)-葡萄糖、蔗糖、D(+)甘露糖、a-甲基-D-半乳糖、D(-)-果糖、L(-)-焦糖、n -乙酰神经氨酸和D(+)-半乳糖。此外,D-葡萄糖醛酸只出现在Apalachicola湾的牡蛎细胞上,而一种与L. odoratus凝集素结合的未知糖既不是D(+)-葡萄糖也不是D(+)-甘露糖,这两种通常的抑制糖都出现在Apalachicola湾和Galveston湾的血细胞上。两个采集地点牡蛎血细胞表面糖质成分的定量和定性差异归因于菌株差异。在无脊椎动物中自然产生的凝集素已被反复提出,除其他功能外,在从非我中识别自我方面发挥作用(Anderson & Good, 1976;程,1984;Cheng et al., 1984;Renwrantz, 1981)。这些蛋白质或糖蛋白包括识别特定糖残基的结合位点。碳水化合物是许多细胞的细胞特性的主要决定因素(Gaveriaux & Loor, 1987),为了鉴定碳水化合物,并通过这样做来增加我们对凝集素介导的生物入侵者的附着或不附着的理解,我们测试了具有已知糖特异性的精选凝集素对美洲牡蛎Crassostrea virginica (Gmelin)血细胞的影响。材料和方法牡蛎。本研究中使用的牡蛎来自两个来源:佛罗里达州的阿巴拉契科拉湾和美国德克萨斯州的加尔维斯顿湾。所有牡蛎都是在1992年2月的最后一周到4月的第二周期间收获的。所有的牡蛎都在3点30分在实验室保存。C在15%的海水中,直到出血前1小时。没有人被关押超过三天。i感谢珍妮特·巴托女士出色的技术援助。本研究由美国商务部国家海洋渔业局拨款(NA16FLO408-01)支持。反式。点。MICROSC。Soc。中国生物医学工程学报,32(2):551 - 557。1993. ? 版权所有,1993年,美国显微学会,Inc。此内容下载自207.46.13.124星期三,2016年6月22日05:21:35 UTC所有使用以http://about.jstor.org/terms TRANS为准。点。MICROSC。SOC。
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引用次数: 12
Tortaguttus stiloensis, a new species of benthic foraminifera from the middle pleistocene of Calabria, Italy 意大利卡拉布里亚中更新世底栖有孔虫类Tortaguttus stiloensis一新种
Pub Date : 1993-04-01 DOI: 10.2307/3226831
R. Patterson, W. Cavazza, J. Blenkinsop
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引用次数: 0
Ultrastructural Study of the Cortex and Membrane Skeleton of Vorticella convallaria (Ciliophora: Peritricha) 小轮虫(纤毛虫科:周缘)皮层和膜骨架的超微结构研究
Pub Date : 1993-04-01 DOI: 10.2307/3226822
R. Wibel, E. Vacchiano, H. E. Buhse
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引用次数: 5
期刊
Transactions of the American Microscopical Society
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