D. Wootton, K. Ryan, R. Demaree, Richard L Critchfield
{"title":"A new species of Gyrodactylus (Monogenea: Monopisthocotylea) on tadpoles of Rana catesbeiana from California, U.S.A.","authors":"D. Wootton, K. Ryan, R. Demaree, Richard L Critchfield","doi":"10.2307/3226681","DOIUrl":"https://doi.org/10.2307/3226681","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"34 1","pages":"230-233"},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86543647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histopathology in a captive yarrow's spiny lizard, Sceloporus jarrovii (Phrynosomatidae), attributed to the mite Hirstiella sp. (Pterygosomatidae)","authors":"S. Goldberg, H. J. Holshuh","doi":"10.2307/3226682","DOIUrl":"https://doi.org/10.2307/3226682","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"16 1","pages":"234-237"},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88673866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Two New Species of Strelkovimermis (Nematoda: Mermithidae) as Imagicidal Parasites of Chironomids (Diptera: Chironomidae) in Lake Itasca, Minnesota, U.S.A.","authors":"Arthur A. Johnson, M. Kleve","doi":"10.2307/3226825","DOIUrl":"https://doi.org/10.2307/3226825","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"20 1","pages":"128-139"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84188632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trophomermis itascensis n. gen., n. sp. (Nematoda: Mermithidae), an imagicidal parasite of Chironomids in Lake Itasca, Minnesota, U.S.A.","authors":"Arthur A. Johnson, M. Kleve","doi":"10.2307/3226824","DOIUrl":"https://doi.org/10.2307/3226824","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"42 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85971398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new tardigrade species, Hypsibius roanensis, is described from epiphytic mosses and lichens on beech trees on Roan Mountain, Tennessee. It differs from other species of the genus by the presence of a sculptured cuticle with irregular tubercles that increase in size caudally. The buccopharyngeal apparatus consists of a narrow buccal tube, well-developed apophyses, and a spherical pharynx with two granular macroplacoids. Roan Mountain is the highest cross range between the Unaka Mountains and the Blue Ridge Mountains in the Southern Appalachians. The north-facing slope of the mountain is in Carter County, Tennessee; the south-facing slope is in Mitchell County, North Carolina. Tardigrades were first recorded from Roan Mountain by Riggin (1962), who found three species of Macrobiotus at 1,890 m in Tennessee. Maucci (1987) reported 10 species in five genera (Macrobiotus, Diphascon, Minibiotus, Itaquascon, and Milnesium) from mosses collected in the spruce-fir forest of Roan Mountain above 1,829 m. The most extensive study of tardigrades on Roan Mountain was by Nelson (1973, 1975), who examined the ecological distribution of 21 species from epiphytic mosses on beech trees at approximately 1,219 m, 1,524 m, and 1,654 m on both the northand southfacing slopes. Nelson found nine specimens of a new species of Hypsibius (designated Hypsibius "a" in Nelson, 1975). However, multiple specimens from a sample were mounted per slide in a non-permanent medium (Turtox CMC9), and the slides were unsuitable for identification after 10 years. McGlothlin (1990) collected again at two of Nelson's sites to determine the long-term stability of tardigrade populations and rediscovered the new species of Hypsibius. The new species is described here. MATERIALS AND METHODS On 8 September 1988, epiphytes (primarily mosses) were collected from beech trees at approximately 1,524 m and 1,654 m on the north-facing slope of Roan Mountain, Carter County, Tennessee (Nelson's stations 5N and 6N). Fifty-six samples were collected, 27 from the 5N station and 29 from 6N. Each sample was placed in a stoppered funnel and soaked in tap water for 24 h to allow the animals to become active and then go into anoxybiosis. The epiphyte was removed and squeezed over the funnel to recover the maximal number of tardigrades. The contents of the funnel were drained into a beaker, and the top layer of water was decanted. Boiling water was added to the beaker to kill the tardigrades. Then the contents of the beaker were rinsed through two sieves, TRANS. AM. MICROSC. SOC., 112(2): 140-144. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.127 on Tue, 28 Jun 2016 06:58:43 UTC All use subject to http://about.jstor.org/terms VOL. 112, NO. 2, APRIL 1993 no. 18 mesh (1 mm) and no. 325 mesh (0.045 mm), simultaneously. The material remaining on the no. 325 sieve was washed into a jar with 95% ethanol. Later, the contents of the jars were examined in a
{"title":"A new species of Hypsibius (Phylum Tardigrada) from Roan Mountain, Tennessee, U.S.A.","authors":"D. Nelson, Karen L. McGlothlin","doi":"10.2307/3226827","DOIUrl":"https://doi.org/10.2307/3226827","url":null,"abstract":"A new tardigrade species, Hypsibius roanensis, is described from epiphytic mosses and lichens on beech trees on Roan Mountain, Tennessee. It differs from other species of the genus by the presence of a sculptured cuticle with irregular tubercles that increase in size caudally. The buccopharyngeal apparatus consists of a narrow buccal tube, well-developed apophyses, and a spherical pharynx with two granular macroplacoids. Roan Mountain is the highest cross range between the Unaka Mountains and the Blue Ridge Mountains in the Southern Appalachians. The north-facing slope of the mountain is in Carter County, Tennessee; the south-facing slope is in Mitchell County, North Carolina. Tardigrades were first recorded from Roan Mountain by Riggin (1962), who found three species of Macrobiotus at 1,890 m in Tennessee. Maucci (1987) reported 10 species in five genera (Macrobiotus, Diphascon, Minibiotus, Itaquascon, and Milnesium) from mosses collected in the spruce-fir forest of Roan Mountain above 1,829 m. The most extensive study of tardigrades on Roan Mountain was by Nelson (1973, 1975), who examined the ecological distribution of 21 species from epiphytic mosses on beech trees at approximately 1,219 m, 1,524 m, and 1,654 m on both the northand southfacing slopes. Nelson found nine specimens of a new species of Hypsibius (designated Hypsibius \"a\" in Nelson, 1975). However, multiple specimens from a sample were mounted per slide in a non-permanent medium (Turtox CMC9), and the slides were unsuitable for identification after 10 years. McGlothlin (1990) collected again at two of Nelson's sites to determine the long-term stability of tardigrade populations and rediscovered the new species of Hypsibius. The new species is described here. MATERIALS AND METHODS On 8 September 1988, epiphytes (primarily mosses) were collected from beech trees at approximately 1,524 m and 1,654 m on the north-facing slope of Roan Mountain, Carter County, Tennessee (Nelson's stations 5N and 6N). Fifty-six samples were collected, 27 from the 5N station and 29 from 6N. Each sample was placed in a stoppered funnel and soaked in tap water for 24 h to allow the animals to become active and then go into anoxybiosis. The epiphyte was removed and squeezed over the funnel to recover the maximal number of tardigrades. The contents of the funnel were drained into a beaker, and the top layer of water was decanted. Boiling water was added to the beaker to kill the tardigrades. Then the contents of the beaker were rinsed through two sieves, TRANS. AM. MICROSC. SOC., 112(2): 140-144. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.127 on Tue, 28 Jun 2016 06:58:43 UTC All use subject to http://about.jstor.org/terms VOL. 112, NO. 2, APRIL 1993 no. 18 mesh (1 mm) and no. 325 mesh (0.045 mm), simultaneously. The material remaining on the no. 325 sieve was washed into a jar with 95% ethanol. Later, the contents of the jars were examined in a ","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"62 1","pages":"140-144"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86021946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Glochidial host of Alasmidonta atropurpurea (Bivalvia: Unionoidea, Unionidae)","authors":"M. E. Gordon, J. Layzer","doi":"10.2307/3226828","DOIUrl":"https://doi.org/10.2307/3226828","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"7 1","pages":"145-150"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79350189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Adhesion Seams in the Tiedemann's Diverticula of the Starfish Henricia sanguinolenta","authors":"J. C. Ferguson, C. Walker","doi":"10.2307/3226830","DOIUrl":"https://doi.org/10.2307/3226830","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"19 1","pages":"158-167"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88920742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The saccharidal constituents of the surfaces of hemocytes of the American oyster, Crassostrea virginica, from Apalachicola Bay, Florida, and Galveston Bay, Texas, were determined by employing eight lectins with known sugar specificities (Con A, Tetragonolobus purpureas, Limulus polyphemus, Dilichos biflorus, Sambucas nigra, Glycine max, Triticum vulgaris, and Lathyrus odoratus). Known inhibiting sugar residues were used to inhibit clumping of lectin-treated oyster hemocytes. As a result, the following saccharides were demonstrated to occur on oyster hemocytes: N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, methyl-a-D-mannopyranoside, D(+)-glucose, sucrose, D(+)mannose, a-methyl-D-galactoside, -D(-)-fructose, L(-)-fucose, N-acetylneuraminic acid, and D(+)-galactose. In addition, D-glucuronic acid occurred only on oyster cells from Apalachicola Bay and an unidentified sugar that binds to the L. odoratus lectin that is neither D(+)-glucose nor D(+)-mannose, the usual inhibiting sugars, occurs on hemocytes from both Apalachicola and Galveston Bays. Quantitative and qualitative differences in the saccharidal constituents on the surfaces of hemocytes from oysters from the two collecting sites are attributed to strain differences. Naturally occurring lectins in invertebrates have been repeatedly proposed, among other functions, to play a role in the recognition of self from nonself (Anderson & Good, 1976; Cheng, 1984; Cheng et al., 1984; Renwrantz, 1981). These proteins or glycoproteins include binding sites that recognize specific sugar residues. In order to identify the carbohydrates, which are the primary determinants of cellular identity of many cells (Gaveriaux & Loor, 1987), and by so doing increase our understanding of lectin-mediated attachment or nonattachment of biotic invaders, we have tested the effects of selected lectins with known saccharidal specificities on hemocytes of the American oyster, Crassostrea virginica (Gmelin). MATERIALS AND METHODS Oysters. The oysters employed in this study originated from two sources: Apalachicola Bay, Florida, and Galveston Bay, Texas, U.S.A. All were harvested during the last week in February through the second week in April 1992. All oysters were held in the laboratory at 3?C in 15% seawater until 1 h prior to bleeding. None was held for more than three days. i The outstanding technical assistance by Ms. Janet M. Barto is gratefully acknowledged. This research was supported by a grant (NA16FLO408-01) from the National Marine Fisheries Service, U.S. Department of Commerce. TRANS. AM. MICROSC. Soc., 112(2): 151-157. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 207.46.13.124 on Wed, 22 Jun 2016 05:21:35 UTC All use subject to http://about.jstor.org/terms TRANS. AM. MICROSC. SOC.
{"title":"Lectin-binding differences on hemocytes of two geographic strains of the American oyster, Crassostrea virginica","authors":"T. Cheng, W. Dougherty, V. Burrell","doi":"10.2307/3226829","DOIUrl":"https://doi.org/10.2307/3226829","url":null,"abstract":"The saccharidal constituents of the surfaces of hemocytes of the American oyster, Crassostrea virginica, from Apalachicola Bay, Florida, and Galveston Bay, Texas, were determined by employing eight lectins with known sugar specificities (Con A, Tetragonolobus purpureas, Limulus polyphemus, Dilichos biflorus, Sambucas nigra, Glycine max, Triticum vulgaris, and Lathyrus odoratus). Known inhibiting sugar residues were used to inhibit clumping of lectin-treated oyster hemocytes. As a result, the following saccharides were demonstrated to occur on oyster hemocytes: N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, methyl-a-D-mannopyranoside, D(+)-glucose, sucrose, D(+)mannose, a-methyl-D-galactoside, -D(-)-fructose, L(-)-fucose, N-acetylneuraminic acid, and D(+)-galactose. In addition, D-glucuronic acid occurred only on oyster cells from Apalachicola Bay and an unidentified sugar that binds to the L. odoratus lectin that is neither D(+)-glucose nor D(+)-mannose, the usual inhibiting sugars, occurs on hemocytes from both Apalachicola and Galveston Bays. Quantitative and qualitative differences in the saccharidal constituents on the surfaces of hemocytes from oysters from the two collecting sites are attributed to strain differences. Naturally occurring lectins in invertebrates have been repeatedly proposed, among other functions, to play a role in the recognition of self from nonself (Anderson & Good, 1976; Cheng, 1984; Cheng et al., 1984; Renwrantz, 1981). These proteins or glycoproteins include binding sites that recognize specific sugar residues. In order to identify the carbohydrates, which are the primary determinants of cellular identity of many cells (Gaveriaux & Loor, 1987), and by so doing increase our understanding of lectin-mediated attachment or nonattachment of biotic invaders, we have tested the effects of selected lectins with known saccharidal specificities on hemocytes of the American oyster, Crassostrea virginica (Gmelin). MATERIALS AND METHODS Oysters. The oysters employed in this study originated from two sources: Apalachicola Bay, Florida, and Galveston Bay, Texas, U.S.A. All were harvested during the last week in February through the second week in April 1992. All oysters were held in the laboratory at 3?C in 15% seawater until 1 h prior to bleeding. None was held for more than three days. i The outstanding technical assistance by Ms. Janet M. Barto is gratefully acknowledged. This research was supported by a grant (NA16FLO408-01) from the National Marine Fisheries Service, U.S. Department of Commerce. TRANS. AM. MICROSC. Soc., 112(2): 151-157. 1993. ? Copyright, 1993, by the American Microscopical Society, Inc. This content downloaded from 207.46.13.124 on Wed, 22 Jun 2016 05:21:35 UTC All use subject to http://about.jstor.org/terms TRANS. AM. MICROSC. SOC.","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"114 1","pages":"151-157"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84015061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tortaguttus stiloensis, a new species of benthic foraminifera from the middle pleistocene of Calabria, Italy","authors":"R. Patterson, W. Cavazza, J. Blenkinsop","doi":"10.2307/3226831","DOIUrl":"https://doi.org/10.2307/3226831","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"8 1","pages":"168-171"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87524672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructural Study of the Cortex and Membrane Skeleton of Vorticella convallaria (Ciliophora: Peritricha)","authors":"R. Wibel, E. Vacchiano, H. E. Buhse","doi":"10.2307/3226822","DOIUrl":"https://doi.org/10.2307/3226822","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"128 1","pages":"107-120"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89303375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}