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Cestoda from Lake fishes in Wisconsin : the morphological identity of plerocercoids of Proteocephalus ambloplitis 威斯康辛湖鱼的尾足类:无翼变形头类尾足类的形态特征
Pub Date : 1992-07-01 DOI: 10.2307/3226608
O. Amin, M. Boarini
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引用次数: 3
A redescription of types of the clam shrimp Eulimnadia agassizii (Spinicaudata : Limnadiidae) 河蚌对虾的种类再描述(棘目:河蚌科)
Pub Date : 1992-07-01 DOI: 10.2307/3226611
Douglas G. Smith
Among the described North American species of the clam shrimp family Limnadiidae, Eulimnadia agassizii is poorly known, having been reported from only two localities in extreme southeastern New England. Though recently reported as nonexistent, the original specimens of this species are in the type collection of the Museum of Comparative Zoology, Harvard University. Specific data regarding the collection and an emended and expanded description of the type material are provided, using traditional and contemporary characters, to complement recent studies of the group. In a revision of the Recent Limnadiidae of North America, Belk (1989) discussed traditional characters used to diagnose species and suggested that egg shell morphology demonstrates less intraspecific variation, thus allowing more confident separation of species. Among the several named forms included in his review was Eulimnadia agassizii, a poorly known species from southeastern New England. E. agassizii was described (as Limnadia) from Penikese Island in what is now the town of Gosnold, Barnstable County, Massachusetts, by Packard (1874a). Along with a stone plaque and a few obscured foundations, the description of E. agassizii is all that remains of an early attempt to establish a marine biology laboratory on the island (later permanently founded at nearby Woods Hole, Massachusetts). Packard (1883) eventually included E. agassizii in his genus Eulimnadia, first formally proposed as a subgenus (Packard, 1847b). Eulimnadia has been recognized by most subsequent authors, although Webb & Bell (1979) reviewed earlier data that suggested overlap in the two taxa and recommended that Eulimnadia be synonymized with Limnadia. Additional study is needed to resolve the two views. Belk (1989) remarked that Packard did not "leave any type material." In fact, the specimens collected by Walter Faxon on Penikese Island, and certainly the same or part of the same collection used by Packard, exists in the Museum of Comparative Zoology, Harvard University, at Cambridge, Massachusetts. The specimens are labeled as "types" and are in the type collection but have not been cataloged. No specific holotype was designated. Unaware of the collection, Belk (1989) used specimens of E. agassizii collected in Woods Hole, Massachusetts, to characterize E. agassizii. Though the Woods Hole collection is useful for purposes of comparison with other species, it nevertheless would be useful to provide an accurate redescription of the type collection. Using type material, I redescribe the species below. Variations in certain characters 11 thank Ellie Armstrong-Prosser of the Grey Museum for kindly loaning their collection of Eulimnadia agassizii for study. Dr. Denton Belk provided comments on different drafts of the manuscript. TRANS. AM. MICROSC. Soc., 111(3): 223-228. 1992. ? Copyright, 1992, by the American Microscopical Society, Inc. This content downloaded from 207.46.13.11 on Wed, 19 Oct 2016 04:41:46 UTC All use s
在已描述的北美蛤虾科物种中,在新英格兰东南部的两个地方只发现了agassizii蛤虾。虽然最近有报道说这个物种不存在,但这个物种的原始标本在哈佛大学比较动物学博物馆的模式收藏中。本文使用传统和当代的文字,提供了关于该集合的具体数据和对字型材料的修订和扩展描述,以补充最近对该群体的研究。Belk(1989)在对North America Recent Limnadiidae的修订中讨论了用于诊断物种的传统特征,并提出蛋壳形态表现出较少的种内变异,从而允许更可靠的物种分离。在他的综述中包括的几种已命名的物种中,有一种是新英格兰东南部的一种鲜为人知的物种——阿加assizii。帕卡德(1874a)将E. agassizii描述为(Limnadia)来自Penikese岛,即现在的马萨诸塞州巴恩斯特布尔县戈斯诺德镇。除了一块石碑和一些模糊不清的地基,阿加西氏E. agassizii的描述是早期试图在岛上建立海洋生物学实验室(后来在马萨诸塞州伍兹霍尔附近永久建立)的全部遗迹。Packard(1883)最终将E. agassizii纳入他的Eulimnadia属,该属最初被正式提出为一个亚属(Packard, 1847b)。尽管Webb和Bell(1979)回顾了早期的数据,认为这两个分类群存在重叠,并建议将Eulimnadia与Limnadia同名,但后来的大多数作者都认可了Eulimnadia。需要进一步的研究来解决这两种观点。Belk(1989)评论说帕卡德没有“留下任何类型的材料”。事实上,沃尔特·法克森在佩尼科斯岛上收集的标本,当然与帕卡德使用的标本相同或部分相同,现存于马萨诸塞州剑桥市哈佛大学比较动物学博物馆。这些标本被标记为“类型”,属于类型集合,但尚未编目。没有指定具体的全型。Belk(1989)在不知情的情况下,使用了在马萨诸塞州伍兹霍尔收集的阿加西乌的标本来描述阿加西乌的特征。虽然伍兹霍尔的收集是有用的目的与其他物种的比较,但它将有助于提供一个准确的重新描述类型收集。使用类型材料,我在下面重新描述物种。感谢格雷博物馆的埃莉·阿姆斯特朗-普罗塞好心地把他们收藏的阿加西草借给我研究。丹顿·贝尔克博士对手稿的不同草稿提供了评论。反式。点。MICROSC。Soc。生物医学工程学报,2011(3):223-228。1992. ? 版权所有,1992年,美国显微学会。本内容下载于207.46.13.11星期三,2016年10月19日04:41:46 UTC所有内容以http://about.jstor.org/terms TRANS为准。点。MICROSC。SOC。由帕卡德(1874a,b, 1883)讨论。基于扫描电子显微镜重新描述了蛋壳和端子形态。材料和方法检查的一系列标本分布在两个批次中,每个批次被指定为类型收集的一部分。这两个拍品都没有编目。收藏中关于标本的唯一书面记录是1873年9月的一份加入日志中的声明,表明W. Faxon捐赠了马萨诸塞州Buzzards湾Penikese岛的一系列标本。这两批都是用70%的乙醇保存的。第一个地块被标记为“游泳池在Penikese岛,Buzzards湾马萨诸塞州1873年8月27日。”沃尔特·法克森著。”第二个标签上写着“乌兰草(unlimnadia agassizii Packard Type) !”这批藏品有196个标本,全部为雌性。第二批标着“乌兰草类型!”帕尼科斯岛,秃鹰湾,1873年8月27日Faxon。”拍卖场中有6个女性标本。从大量标本中取出14个标本,用于提供光和扫描电子显微镜(SEM)研究的身体部位。用于光学显微镜的身体部位被清除并安装在Hoyer培养基中,一种半永久性的安装介质。为了进行扫描电镜检查,鸡蛋和整个telson被脱水,涂覆,并用JEOL型号JSM 3500扫描电子显微镜检查。头部的图纸是使用眼网格确定关键点尺寸的滑动支架的徒手再现。所有准备好的材料,包括载玻片和扫描电镜存根,都被送回哈佛大学比较动物学博物馆的类型收藏中。此外,还对1970年8月9日D. J. Zinn在马萨诸塞州伍兹霍尔海洋生物实验室(gray Museum 994)收集的阿加西海螺标本进行了检查。9个女性标本在收藏中。这些后一种标本的检查仅限于使用光学显微镜。
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引用次数: 8
Significance of Small Organic Chelators in Laboratory Cultures of Ephydatia fluviatilis (Porifera: Spongillidae) 小型有机螯合剂在水生棘虫实验室培养中的意义(多孔目:海绵虫科)
Pub Date : 1992-07-01 DOI: 10.2307/3226606
F. Belas, J. C. Francis, M. Poirrier
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引用次数: 2
Comparative Morphology of Epithemata (Polar Chorionic Structures) in the Eggs of Ephemerella ignita (Ephemeroptera: Ephemerellidae) 蜉蝣(蜉蝣目:蜉蝣科)卵上皮(极性绒毛膜结构)的比较形态学
Pub Date : 1992-07-01 DOI: 10.2307/3226613
E. Gaino, E. Bongiovanni
The eggs of the mayfly Ephemerella ignita (Ephemeroptera: Ephemerellidae) are characterized by a polar adhesive structure, the epithema. The epithema prevents egg drift following deposition in water by extending a network of threads. Mated females lay an aggregate of eggs that is attached to the last abdominal sternites. Within the aggregate, eggs with differently shaped epithemata are arranged in a well-defined pattern. Three types of eggs were defined by three types of epithemata, namely short, bulging, and long. In addition, histological observations confirmed the thread-like composition of the epithemata and their peculiar arrangement in each type. Short and long epithema egg types differed also by their total lengths. Structural changes in the architecture of the epithemata are discussed in relation to a different function of such devices. The fine structure of the egg chorionic pattern in Ephemeroptera has been used, as with other insect orders, to elucidate differences and relationships among species (Alba-Tercedor & Sowa, 1987; Gaino et al., 1987, 1989; Gaino & Mazzini, 1984; Mazzini & Gaino, 1990). In addition, ultrastructural studies on mayfly eggs have indicated the adhesive function of their peculiar projections, which may be involved in preventing egg drift following deposition in water (Gaino & Mazzini, 1987, 1988). As a consequence, shell surface structures may give some insight into the devices developed by different species to survive in their respective habitats (Gaino & Mazzini, 1988). Ootaxonomy is based chiefly on the species-specificity of chorionic architecture, which is constant within the representatives of a species. For instance, the organization of the mayfly egg has been employed to classify the order and to recognize nymph and adult stages in the same species (Koss, 1968). The polar devices, referred to as the "epithema" (this term having been introduced first by Bengtsson, 1913) or "polar cap" (terminology after Koss & Edmunds, 1974), is a unique chorionic structure in eggs of Ephemerella ignita Poda at the posterior polar region and is involved in egg adhesion to the substratum. The occurrence of eggs with adhesive polar devices of different lengths in E. ignita were noted earlier and were considered to be related to the position of the eggs within the ducts (Bengtsson, 1913; Degrange, 1960). This observation might have relevant implications in the field of ootaxonomy and may imply additional functions. The primary objective of this paper is to describe and document the ultraWe thank Prof. C. Falugi and Dr. C. Pesce for their helpful criticisms and F. Di Lauro for his technical assistance. This research was supported by the Italian Ministry for Public Education (M.U.R.S.T.40% and 60% funds). TRANS. AM. MICROSC. SOC., 11 l(3): 255-265. 1992. O Copyright, 1992, by the American Microscopical Society, Inc. 256 TRANS. AM. MICROSC. SOC. structural features of the eggs of E. ignita with emphasis on variations among epithe
蜉蝣(蜉蝣目:蜉蝣科)的卵具有极粘连结构,即卵皮。脓肿通过延伸网状线防止卵在水中沉积后漂移。交配后的雌性会产下一堆卵,这些卵附着在最后的腹部胸骨上。在聚集体内,具有不同形状上皮的卵以明确的模式排列。三种类型的卵被定义为三种类型的上皮,即短,鼓鼓的和长的。此外,组织学观察证实了针状脓肿的组成和它们在每种类型中的特殊排列。短卵和长卵的总长度也不同。讨论了与此类装置的不同功能有关的脓肿结构的结构变化。与其他昆虫目一样,蜉蝣目卵膜图案的精细结构已被用来阐明物种之间的差异和关系(Alba-Tercedor & Sowa, 1987;Gaino et al., 1987, 1989;Gaino & Mazzini, 1984;Mazzini & Gaino, 1990)。此外,对蜉蝣卵的超微结构研究表明,其特殊突起具有粘附功能,这可能与防止卵在水中沉积后漂移有关(Gaino & Mazzini, 1987, 1988)。因此,壳的表面结构可以让我们深入了解不同物种为在各自的栖息地生存而开发的设备(Gaino & Mazzini, 1988)。卵分类学主要基于绒毛膜结构的种特异性,这在一个物种的代表中是恒定的。例如,蜉蝣卵的组织结构已被用于分类,并在同一物种中识别若虫和成虫阶段(Koss, 1968)。极性装置,被称为“epithema”(这个术语由Bengtsson于1913年首次提出)或“polar cap”(来自Koss & Edmunds, 1974年的术语),是星历藻卵中位于后极性区域的独特绒毛膜结构,参与卵与底层的粘附。早前就注意到,在火炬手中出现了具有不同长度的粘附极性装置的卵,并被认为与卵在导管内的位置有关(Bengtsson, 1913;Degrange, 1960)。这一观察结果可能对卵巢分类学领域有相关的启示,并可能暗示其他功能。本文的主要目的是描述和记录超光速。我们感谢C. Falugi教授和C. Pesce博士的有益批评和F. Di Lauro的技术支持。这项研究得到了意大利公共教育部(M.U.R.S.T.40%和60%的资金)的支持。反式。点。MICROSC。SOC。浙江农业学报,11(3):255-265。1992. O版权所有,1992,由美国显微学会,Inc. 256 TRANS。点。MICROSC。SOC。火花菌卵的结构特征,并着重于脓肿间的变异。材料与方法于1990年6月在热那亚Rossiglione附近的Berlino溪流附近海拔约300 m处采集了处于产卵期的蜉蝣成虫。通过附着在腹部的绿色卵团来识别产卵的雌性。鸡蛋用Karnovsky(1965)固定液固定,用三水合乙酸钠缓冲液冲洗。为了进行组织学检查,鸡蛋在分级的乙醇系列中脱水,并包埋在甲基丙烯酸树脂(JB4)中。切片用甲苯胺蓝染色。对于扫描电镜(SEM)检查,选择的材料按照上述程序脱水进行组织学研究,用液态CO在Bomar设备中进行临界点干燥,用银导电涂料附着在样品支架上,并在Balzer Union蒸发器中涂上金钯。用Philips EM 505扫描电镜观察标本。尺寸,包括脓肿,是在120个鸡蛋的样本中测量的,根据脓肿的形态,再分为三组,每组40个鸡蛋。给出的数据是每组40个卵子的平均值。
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引用次数: 11
Cephalic tubercles, a new character useful for the taxonomy of desmoscolecidae (Nematoda) 头结节——一个对线虫科分类有用的新特征
Pub Date : 1992-07-01 DOI: 10.2307/3226610
Y. Shirayama, W. Hope
The cephalic regions of 10 nematode species of the family Desmoscolecidae were examined by scanning electron microscopy (SEM). They belonged to two genera, Tricoma and Desmoscolex, which were collected from off the coasts of Japan, Caribbean Sea, Clew Bay (Ireland), and Thailand. The basic structure of the lip region was the same within Tricoma regardless of geographic region, but a distinct intergeneric difference was found. In species of this genus, six conspicuous outer labial sensilla protruded from the smooth lip surface, and the circular ridges corresponding to the boundary of the closed oral aperture, the external rim of the labia, and the anterior rim of the cephalic concretion ring were distinctive. On the other hand, the labial surface of specimens of the genus Desmoscolex was covered by a variety of small, tuberculate structures. These cephalic tubercles were 70-240 nm in diameter and recognizable only by SEM examination. The shape of the cephalic tubercles and the area of the head covered by them were constant within the same species, but differed among species. The labial sensilla were surrounded and obscured by specialized cephalic tubercles in Desmoscolex. The cephalic tubercles were found only in adults of Desmoscolex. These results suggested that the presence of cephalic tubercles is a synapomorphic character of Desmoscolex, and that it is useful for phylogenetic studies of the Desmoscolecidae. The family Desmoscolecidae is a unique group of free-living nematodes characterized by desmens, which are thick, transverse rings considered to consist of sedimentary particles and cementing materials (desmos). Earlier taxonomic revisions of the family by Timm (1970) and Freudenhammer (1975) were based mainly upon external structures, in particular the shape and number of the desmens and the shape and arrangement of somatic setae on each desmen. Decraemer (1974, 1975a,b, 1977, 1978a,b, 1979, 1984) studied this group extensively; recently, she has revised the order Desmoscolecida using the methods of phylogenetic systematics (cladistics) and character states of both external and internal morphology (Decraemer, 1985). Scanning electron microscopy (SEM) is a most useful technique in taxonomic studies of nematodes (Hirschmann, 1983). This is especially true in studies of Desmoscolecidae because precise observations of external structures are essential for classification within the family. SEM also can reveal details that are not resolved by ordinary light microscopic techniques. During a SEM investigation of deep-sea nematodes collected from the western 1 This research was supported in part by a postdoctoral fellowship awarded to Y. Shirayama from the Smithsonian Institution. The authors thank Abbie Yorkoff, Brian Kahn, Susann Braden, and Walter Brown for their technical assistance. Drs. Robert P. Higgins and Chittima Aryuthaka provided part of the material used in this research. 2 To whom correspondence should be directed. 3 Present address:
用扫描电子显微镜(SEM)对10种棘球蚴科(desmocolecidae)线虫头部区域进行了观察。它们分属Tricoma和Desmoscolex两属,分别采自日本、加勒比海、克鲁湾(爱尔兰)和泰国海岸。唇部的基本结构在不同地理区域的Tricoma内是相同的,但发现明显的属间差异。本属种光滑唇面突出6个明显的唇外感受器,与口孔闭合边界、唇外缘、头结环前缘相对应的圆脊明显。另一方面,desmocolex属标本的唇表面覆盖着各种小的结核状结构。这些头结节直径为70-240 nm,仅通过扫描电镜检查可识别。头结节的形状及其覆盖的头部面积在同一种内是不变的,但在不同种之间是不同的。唇感器被特殊的头结节包围和遮蔽。头部结节仅见于成虫。这些结果表明,头结节的存在是桥霉菌的一个突触性特征,这对桥霉菌科的系统发育研究是有益的。桥丝虫科是一种独特的自由生活的线虫,其特征是桥丝,它是厚的,横向的环,被认为是由沉积颗粒和胶结物质(桥丝)组成。Timm(1970)和Freudenhammer(1975)对该科的早期分类修订主要基于外部结构,特别是节的形状和数量以及每个节上的体刚毛的形状和排列。Decraemer (1974,1975a,b, 1977, 1978a,b, 1979,1984)对这一群体进行了广泛的研究;最近,她利用系统发育系统学(分支学)和外部形态和内部形态特征状态的方法修订了desmocolecida目(Decraemer, 1985)。扫描电子显微镜(SEM)是线虫分类学研究中最有用的技术(Hirschmann, 1983)。这在对桥霉菌科的研究中尤其如此,因为对外部结构的精确观察对该科的分类至关重要。扫描电镜还可以揭示普通光显微技术无法解决的细节。在对从西部采集的深海线虫进行扫描电镜调查时,这项研究得到了史密森学会授予Y. Shirayama的博士后奖学金的部分支持。作者感谢Abbie Yorkoff、Brian Kahn、Susann Braden和Walter Brown提供的技术援助。Drs。Robert P. Higgins和Chittima Aryuthaka提供了本研究中使用的部分材料。信函应发给谁。3目前地址:日本东京164中野区南代东京大学海洋研究所。反式。点。MICROSC。Soc。科学通报,2011(3):211-222。1992. ? 版权所有,1992年,美国显微学会。此内容从157.55.39.162下载于星期四,2016年8月11日04:49:29 UTC所有内容以http://about.jstor.org/terms TRANS为准。点。MICROSC。SOC。在太平洋,我们在三个未被描述的Desmoscolex物种的唇区发现了以前未知的超微结构。我们还检查了从大西洋和印度洋较浅的深度收集的其他桥藻,以确定该结构的一般情况。本文的主要目的是描述所有种类的唇虱的唇部超微结构。本文还讨论了这种结构在桥孢属中的分布,并讨论了这种结构对该科分类的价值。材料与方法研究了5组桥孢子线虫(表1),这些线虫来自世界各地,包括西北太平洋、北大西洋、东印度洋和加勒比海。这些藏品代表了从潮下到深海的深度。所研究的标本代表2属、4亚属和10种(表II)。选择Tricoma属的4种代表Tricominae亚科;选取6种链霉菌作为链霉菌科的代表。这两个属都包括来自不同地理区域的物种代表。这两个属的代表都是从日本东北部三陆附近的地区带走的。标本中有Tricoma亚属1种,Quadricoma亚属3种,Desmoscolex亚属6种,Desmolorenzenia亚属1种。用63米筛网从沉积物中提取标本,用10%福尔马林固定保存在海水中。
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引用次数: 4
Ultrastructure of the Penis Papilla and Antrum of Dugesia tigrina (Platyhelminthes: Tricladida) 虎足足足虫阴茎、乳头和胃窦的超微结构
Pub Date : 1992-07-01 DOI: 10.2307/3226607
W. Fischlschweiger
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引用次数: 4
Pterocystis ebelii, a new species of heliozoa from India 标题印度翼虫属一新种
Pub Date : 1992-04-01 DOI: 10.2307/3226670
D. Wujek, P. Elsner
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引用次数: 6
Nuclear fine structure during development in Heliophrya sp. (Ciliophora : Suctoria) Heliophrya sp.(纤毛虫目:苏陀利亚)发育过程中的核精细结构
Pub Date : 1992-04-01 DOI: 10.2307/3226669
D. P. Fox, Bruce F. Hill, G. Chapman
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引用次数: 0
Nitrogen Fixation: Achievements and Objectives 固氮:成就和目标
Pub Date : 1992-04-01 DOI: 10.2307/3226673
W. S. Silver, P. Gresshoff, L. Roth, G. Stacey, W. Newton
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引用次数: 259
Biophysical Electron Microscopy 生物物理电子显微镜
Pub Date : 1992-04-01 DOI: 10.2307/3226674
G. Chapman, P. Hawkes, U. Valdré
{"title":"Biophysical Electron Microscopy","authors":"G. Chapman, P. Hawkes, U. Valdré","doi":"10.2307/3226674","DOIUrl":"https://doi.org/10.2307/3226674","url":null,"abstract":"","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"18 1","pages":"167"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80107915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
期刊
Transactions of the American Microscopical Society
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