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[Application of plasmatrix in improving peri-implant soft tissue phenotype]. 血浆基质在改善种植体周围软组织表型中的应用
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20251015-00406
H Zeng, Y L Wang, Y F Zhang

The phenotype of peri-implant soft tissue is crucial to the long-term treatment outcome of implant restoration, but soft tissue phenotype deficiencies are common in clinical practice and need to be improved through soft tissue augmentation. Although autologous soft tissue graft is still the gold standard for soft tissue augmentation, it has limitations such as limited donor area, increased trauma, and low patient acceptance. Plasmatrix has sufficient sources, is easy to prepare, and has a scaffold structure, growth factors, and cell components that support soft tissue growth. It can be used to improve the phenotype of peri-implant soft tissue. However, there are currently limited clinical studies on the application of plasmatrix in improving the phenotype of peri-implant soft tissue, and there is a lack of consensus conclusions, which makes the majority of clinicians feel confused when using plasmatrix. This article will try to combine existing clinical studies and the clinical experience of the author's team to explain the classification of peri-implant soft tissue phenotypes and the application of plasmatrix in improving soft tissue phenotypes, in order to provide a reference for related clinical treatments.

种植体周围软组织的表型对种植体修复的长期治疗效果至关重要,但在临床实践中软组织表型缺陷是常见的,需要通过软组织增强来改善。虽然自体软组织移植仍然是软组织增强的金标准,但它存在供体面积有限、创伤增加、患者接受度低等局限性。质基质来源充足,制备简单,具有支架结构、生长因子和支持软组织生长的细胞成分。可用于改善种植体周围软组织的表型。然而,目前关于plasmatrix在改善种植体周围软组织表型中的应用的临床研究有限,缺乏一致的结论,这使得大多数临床医生在使用plasmatrix时感到困惑。本文将尝试结合现有临床研究和作者团队的临床经验,阐述种植体周围软组织表型的分类及plasmatrix在改善软组织表型中的应用,以期为相关临床治疗提供参考。
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引用次数: 0
[The role of exosomal miRNA in the progression of oral squamous cell carcinoma and its molecular mechanism]. [外泌体miRNA在口腔鳞状细胞癌进展中的作用及其分子机制]。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20251015-00405
C X Li, N B Zheng, C G Wang, Z C Gong

Oral cancer, as one kind of mucosal epithelial tumor, constitutes approximately 2% of all cancers, while the most common type, oral squamous cell carcinoma (OSCC) represents around 90% histopathology of oral cancers. Although the treatment of OSCC has been improved in recent 20 years, its 5-year survival rate has not raised significantly. The crux to improve the survival rate and prognosis of OSCC patients lies in the early diagnosis and intervention of this disease. Hence, exploring new diagnostic and therapeutic strategies for OSCC is therefore an urgent priority. Exosomes, the small membrane vesicles originated from endosomes, have been detected in a wide array of bodily fluids. Exosomes have biological properties of derived cells based on containing a diversity of proteins, lipids, DNA fragments, mRNAs, and non-coding RNAs, including microRNAs, long non-coding RNAs, piRNAs, circular RNAs, tsRNAs, and ribosomal RNAs, which are delivered to neighboring cells or even transported to distant sites. They participate in cellular communication as well as play an important role in many diseases and immune response. Exosomes have been associated with the tumorigenesis of OSCC, promoting the proliferation, colonization, and metastasis of OSCC by transferring their cargos to the target cells. Furthermore, exosomes participate in the regulation of the tumor microenvironment to affect cancer progression in vivo. In this review, we summarize the crucial role of exosomes in the tumorigenesis and progression of OSCC and discuss the potential clinical application of exosomes in OSCC treatment.

口腔癌作为粘膜上皮性肿瘤的一种,约占所有癌症的2%,而最常见的口腔鳞状细胞癌(Oral squamous cell carcinoma, OSCC)约占口腔癌组织病理学的90%。虽然近20年来OSCC的治疗方法有所改善,但其5年生存率并没有明显提高。早期诊断和干预是提高OSCC患者生存率和预后的关键。因此,探索新的OSCC诊断和治疗策略是当务之急。外泌体是一种起源于内泌体的小膜囊泡,已在多种体液中被检测到。外泌体含有多种蛋白质、脂质、DNA片段、mrna和非编码rna,包括microrna、长链非编码rna、pirna、环状rna、tsrna和核糖体rna,具有衍生细胞的生物学特性,它们被递送到邻近细胞甚至转运到远处。它们参与细胞通讯,并在许多疾病和免疫反应中发挥重要作用。外泌体与OSCC的肿瘤发生有关,通过将其货物转移到靶细胞,促进OSCC的增殖、定植和转移。此外,外泌体参与肿瘤微环境的调节,影响体内癌症的进展。在这篇综述中,我们总结了外泌体在OSCC肿瘤发生和发展中的重要作用,并讨论了外泌体在OSCC治疗中的潜在临床应用。
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引用次数: 0
[Interpretation of World Federation of Orthodontists guidelines for postgraduate orthodontic education: curriculum construction]. 【世界正畸医师联合会研究生正畸教育指南解读:课程建设】。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20250914-00361
Z J Liu, H He

As a branch of stomatology, the professional education of orthodontics has always had its own distinct characteristics and rules. In 2023, the World Federation of Orthodontists (WFO) published the second edition of its Guidelines for Postgraduate Education in Orthodontics in its official journal. This document outlines detailed requirements and recommendations regarding various aspects of orthodontic postgraduate education, including training objectives, curriculum design, and faculty qualifications. This paper aims to interpret the curriculum construction section of the WFO guidelines and to explore the key differences between current orthodontic education in China and that in Western countries. Through this comparative analysis, it seeks to provide insights for improving undergraduate orthodontic teaching, postgraduate and continuing orthodontic education, and to inform future directions for innovation in orthodontic training in China.

正畸专业教育作为口腔医学的一个分支,一直有自己鲜明的特点和规律。2023年,世界正畸医师联合会(World Federation of Orthodontists, WFO)在其官方期刊上发布了第二版《正畸研究生教育指南》。本文件概述了关于正畸研究生教育的各个方面的详细要求和建议,包括培训目标,课程设计和教师资格。本文旨在解读WFO指南的课程建设部分,并探讨目前中国正畸教育与西方国家的主要差异。通过这一对比分析,旨在为改善本科正畸教学、研究生正畸教育和继续正畸教育提供见解,并为未来中国正畸培训的创新方向提供信息。
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引用次数: 0
[Introduction and interpretation of the 2024 consensus report of the second European Consensus Workshop on education in periodontology]. [第二届欧洲牙周病教育共识研讨会2024年共识报告的介绍和解释]。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20250623-00232
F X Geng, J L Shao, Y Xu, W J Hu, L Lin, S H Ge, Y P Pan

The European Federation of Periodontology (EFP) and the Association for Dental Education in Europe (ADEE) jointly held the second European Consensus Workshop on Education in Periodontology in February, 2023. Building on the first consensus workshop in 2009, expert working groups from European Workshop Committee updated four educational levels: undergraduate, specialist, continuing professional development (CPD), as well as teaching methods, culminating in the updated consensus report in March, 2024. This article introduces and interprets the contents of the 2024 consensus report. Specific to China's national conditions, we also propose future perspectives and considerations on different levels of periodontal education in China based on this consensus.

欧洲牙周病联合会(EFP)和欧洲牙科教育协会(ADEE)于2023年2月联合举办了第二届欧洲牙周病教育共识研讨会。在2009年第一次共识研讨会的基础上,来自欧洲研讨会委员会的专家工作组更新了四个教育水平:本科、专科、持续专业发展(CPD)以及教学方法,最终于2024年3月发布了更新的共识报告。本文对《2024年共识报告》的内容进行了介绍和解读。针对中国国情,在此基础上提出了未来中国不同牙周教育水平的展望和思考。
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引用次数: 0
[Preliminary application and outcomes of the single-incision 'axial' localization approach in fully endoscopic parotidectomy]. 【单切口“轴向”定位入路在全内镜腮腺切除术中的初步应用及效果】。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20250601-00199
Z J Gao, T T Gao, Y Y Jian, N Gao, K Fu, J J Sun, W He

To investigate the feasibility and advantages of "Axis Coordinate Localization Method" combined with total endoscopic technique for parotid surgery. A total of 31 patients with parotid gland tumors who were admitted to the First Affiliated Hospital of Zhengzhou University from January 2022 to August 2024 were included in this study. The cohort comprised 11 males (35.5%) and 20 females (64.5%), with age of (38.87±13.90) years and a mean maximum tumor diameter of (19.34±6.89) mm. Among these cases, 93.5% (29/31) involved the superficial lobe and 6.5%(2/31) the deep lobe of the parotid gland. The "axial" localization method was utilized to plan the surgical approach: establishing a coordinate system with the earlobe as the origin, the surface projection of the parotid duct as the x-axis, and a perpendicular line through the origin as the y-axis. The incision type-pre-tragal, retroauricular groove, or postauricular hairline-was selected according to the position of the tumor's surface projection center within this coordinate system. All operations were performed using a complete endoscopic technique for tumor and glandular tissue resection. Postoperative facial nerve function was assessed with the House-Brackmann grading scale, while patient incision satisfaction, impact on work and daily life, and facial symmetry were quantitatively evaluated using a Numerical Rating Scale (10-point system). Results: All 31 patients successfully underwent the procedure. Short-term postoperative complications included temporary facial paralysis in 1 case (3.2%), periauricular numbness in 1 case (3.2%), surgical site effusion in 1 case (3.2%), and subcutaneous hematoma in 1 case (3.2%). All complications resolved after appropriate management. During follow-up, there were no instances of sialocele, Frey's syndrome, incision infection, or tumor recurrence. Patient ratings were as follows: incision satisfaction (9.42±0.78), impact of surgery on daily life (1.32±0.60), and facial symmetry (9.29±0.78). The one case of temporary facial paralysis recovered 3 months after surgery. The combination of the "Axial" localization method and fully endoscopic technique demonstrates clear clinical feasibility in parotid tumor resection. This approach enables rapid and personalized incision selection based on tumor location, while the endoscopic minimally invasive technique reduces tissue trauma, improves cosmetic outcomes, and enhances surgical safety. It provides a new minimally invasive treatment pathway for patients with benign parotid tumors.

探讨“轴坐标定位法”联合全内镜技术在腮腺手术中的可行性及优势。本研究选取2022年1月至2024年8月郑州大学第一附属医院住院的腮腺肿瘤患者31例。男性11例(35.5%),女性20例(64.5%),年龄(38.87±13.90)岁,平均最大肿瘤直径(19.34±6.89)mm,其中93.5%(29/31)累及腮腺浅叶,6.5%(2/31)累及腮腺深叶。采用“轴向”定位方法规划手术入路:以耳垂为原点,腮腺导管表面投影为x轴,通过原点的垂直线为y轴,建立坐标系。根据肿瘤表面投影中心在该坐标系内的位置选择切口类型——耳前沟、耳后沟或耳后发际线。所有手术均采用完整的内镜技术切除肿瘤和腺组织。采用House-Brackmann评分法评估术后面神经功能,采用数值评定量表(10分制)定量评估患者切口满意度、对工作和日常生活的影响及面部对称性。结果:31例患者均顺利完成手术。术后短期并发症包括暂时性面瘫1例(3.2%),耳周麻木1例(3.2%),手术部位积液1例(3.2%),皮下血肿1例(3.2%)。所有并发症均经适当处理后消失。随访期间,无涎腺囊肿、弗雷氏综合征、切口感染或肿瘤复发。患者评分如下:切口满意度(9.42±0.78),手术对日常生活的影响(1.32±0.60),面部对称性(9.29±0.78)。1例暂时性面瘫术后3个月恢复。“轴向”定位法与全内镜技术相结合,在腮腺肿瘤切除术中具有明确的临床可行性。这种方法可以根据肿瘤位置快速和个性化地选择切口,而内镜微创技术减少了组织创伤,改善了美容效果,并提高了手术安全性。为腮腺良性肿瘤患者提供了一种新的微创治疗途径。
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引用次数: 0
[Consensus on informed consent for orthodontic treatment]. [正畸治疗知情同意共识]。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20251013-00402
Y Cao, B Fang, Z L Jin, H He, Y X Bai, L Wang, H P Lu, Z H Zhao, T M Xu, W R Li, M Hu, J L Song, J Wang, F Jin, D Bai, X L Han, Y H Liu, B Yan, J Guo, J J Shi, Y M Li, Z H Li, X P Wu, J T Hu, L Y Xu, L Liu, Y Liu, Y Q Lu, W W Ma, S X Mo, L L Ren, S X Cui, Y J Fan, J G Xu, L L Xu, Z J Zheng, P J Wang, R Zou, C F Liu, L G Xia, L Hu, W C Wang, L P Wu, X X Kou, J L Tan, Y B Liu, B W Meng, Y T Hao, L L Chen

This consensus was developed by the Orthodontic Society of the Chinese Stomatological Association to provide a systematic, scientific, and practical guideline for informed consent in orthodontic care. Orthodontic treatment is typically lengthy, highly individualized, and involves multiple factors such as growth and development, occlusal function, and facial esthetics. Rapid technological advances and diverse risk profiles make the traditional reliance on orthodontist experience or institutional templates insufficient to ensure patients' full understanding and autonomous decision-making. To address this, the expert panel conducted extensive reviews of domestic and international guidelines, analyzed representative dispute cases, and performed multicenter patient-clinician surveys. Using a multi-round Delphi method, the group established a standardized informed consent framework covering the initial consultation, treatment, and retention phases. The consensus emphasizes that informed consent is not only a fundamental legal and ethical requirement but also a key step in building trust, improving patient compliance, and enhancing treatment satisfaction. Orthodontists should clearly and comprehensively explain treatment plans, potential risks, uncertainties, and associated costs, while respecting the autonomy of patients or guardians, and maintain continuous communication and dynamic evaluation throughout the treatment process. The release of this consensus provides unified and authoritative guidance for clinical orthodontics, helping to standardize informed consent, enhance its transparency, safeguard patient rights, reduce medical risks, and promote high-quality, sustainable development of orthodontic practice.

这一共识是由中国口腔医学会正畸学会制定的,旨在为正畸治疗中的知情同意提供一个系统、科学和实用的指南。正畸治疗通常是漫长的,高度个性化的,涉及多种因素,如生长发育,咬合功能和面部美学。快速的技术进步和多样化的风险特征使得传统上对正畸医生经验或机构模板的依赖不足以确保患者充分理解和自主决策。为了解决这个问题,专家小组对国内和国际指南进行了广泛的审查,分析了具有代表性的争议案例,并进行了多中心患者-临床医生调查。采用多轮德尔菲法,该小组建立了一个标准化的知情同意框架,涵盖了初始咨询、治疗和保留阶段。共识强调,知情同意不仅是一项基本的法律和伦理要求,也是建立信任、提高患者依从性和提高治疗满意度的关键步骤。正畸医师应在尊重患者或监护人自主权的前提下,清晰全面地说明治疗方案、潜在风险、不确定性和相关费用,并在整个治疗过程中保持持续沟通和动态评估。本共识的发布为临床正畸提供了统一、权威的指导,有助于规范知情同意,提高知情同意透明度,维护患者权益,降低医疗风险,促进正畸实践高质量、可持续发展。
{"title":"[Consensus on informed consent for orthodontic treatment].","authors":"Y Cao, B Fang, Z L Jin, H He, Y X Bai, L Wang, H P Lu, Z H Zhao, T M Xu, W R Li, M Hu, J L Song, J Wang, F Jin, D Bai, X L Han, Y H Liu, B Yan, J Guo, J J Shi, Y M Li, Z H Li, X P Wu, J T Hu, L Y Xu, L Liu, Y Liu, Y Q Lu, W W Ma, S X Mo, L L Ren, S X Cui, Y J Fan, J G Xu, L L Xu, Z J Zheng, P J Wang, R Zou, C F Liu, L G Xia, L Hu, W C Wang, L P Wu, X X Kou, J L Tan, Y B Liu, B W Meng, Y T Hao, L L Chen","doi":"10.3760/cma.j.cn112144-20251013-00402","DOIUrl":"https://doi.org/10.3760/cma.j.cn112144-20251013-00402","url":null,"abstract":"<p><p>This consensus was developed by the Orthodontic Society of the Chinese Stomatological Association to provide a systematic, scientific, and practical guideline for informed consent in orthodontic care. Orthodontic treatment is typically lengthy, highly individualized, and involves multiple factors such as growth and development, occlusal function, and facial esthetics. Rapid technological advances and diverse risk profiles make the traditional reliance on orthodontist experience or institutional templates insufficient to ensure patients' full understanding and autonomous decision-making. To address this, the expert panel conducted extensive reviews of domestic and international guidelines, analyzed representative dispute cases, and performed multicenter patient-clinician surveys. Using a multi-round Delphi method, the group established a standardized informed consent framework covering the initial consultation, treatment, and retention phases. The consensus emphasizes that informed consent is not only a fundamental legal and ethical requirement but also a key step in building trust, improving patient compliance, and enhancing treatment satisfaction. Orthodontists should clearly and comprehensively explain treatment plans, potential risks, uncertainties, and associated costs, while respecting the autonomy of patients or guardians, and maintain continuous communication and dynamic evaluation throughout the treatment process. The release of this consensus provides unified and authoritative guidance for clinical orthodontics, helping to standardize informed consent, enhance its transparency, safeguard patient rights, reduce medical risks, and promote high-quality, sustainable development of orthodontic practice.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 12","pages":"1327-1336"},"PeriodicalIF":0.0,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Microimplant-assisted rapid palatal expansion treatment of a case of skeletal class Ⅲ malocclusion]. 微种植体辅助快速腭扩张治疗骨骼类Ⅲ错颌畸形1例。
Q4 Medicine Pub Date : 2025-11-28 DOI: 10.3760/cma.j.cn112144-20250814-00318
H J Song, J Y Song, Y Li, T Y Qiu, D F Luo, M Hu
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引用次数: 0
[Application status of three-dimensional facial scanning technology in stomatology]. 三维面部扫描技术在口腔医学中的应用现状
Q4 Medicine Pub Date : 2025-11-09 DOI: 10.3760/cma.j.cn112144-20250515-00182
T R Wang, S Y Wang, W Q Cui, X Qin, L Y Xu

With the continuous development of digital technology, three-dimensional (3D) facial scanning technology is becoming more and more widely used in dentistry, where it enhances the precision of various processes of clinical diagnosis and treatment, and provides new perspectives and tools for dental research and application. Nowadays, 3D facial scanning technology meets clinical needs in terms of accuracy, and most facial scanners have an accuracy of 140-1 330 μm, and the 3D facial scanning related applications of smartphones can also meet the clinical application needs to a certain extent. In the clinical field, the 3D scanner can be combined with a variety of technologies to achieve multi-modal data fusion and create virtual patients, and its prediction rate in obstructive sleep apnea hypopnea syndrome is as high as 91%, which has great clinical application value. This article aims to explore the research status, clinical applications, and accuracy analysis of 3D facial scanning techniques in the field of dentistry.

随着数字技术的不断发展,三维面部扫描技术在牙科领域的应用越来越广泛,提高了临床诊疗各环节的精度,为牙科研究和应用提供了新的视角和工具。目前,3D面部扫描技术在精度上满足临床需求,大多数面部扫描仪的精度在140-1 330 μm之间,智能手机的3D面部扫描相关应用也能在一定程度上满足临床应用需求。在临床领域,3D扫描仪可结合多种技术实现多模态数据融合,创建虚拟患者,其对阻塞性睡眠呼吸暂停低通气综合征的预测率高达91%,具有很大的临床应用价值。本文旨在探讨三维面部扫描技术在牙科领域的研究现状、临床应用及准确性分析。
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引用次数: 0
[Research progress in dental pulp assessment and diagnosis systems for pulpitis]. 牙髓炎牙髓评估与诊断系统的研究进展
Q4 Medicine Pub Date : 2025-11-09 DOI: 10.3760/cma.j.cn112144-20250604-00204
J Liao, S Y Liu

Accurate assessment of pulp status is crucial for formulating treatment strategies for affected teeth. Traditional assessment methods include subjective symptoms, clinical examination, and imaging findings, despite their diagnostic accuracy is controversial. In recent years, new technologies such as pulp blood flow testing, pulp inflammation biomarkers and artificial intelligence-assisted diagnosis have been introduced, providing more objective and precise diagnostic tools. This review systematically examines pulp status assessment methods now available, explores their clinical diagnostic effectiveness and limitations, and reviews current pulpitis diagnosis systems to optimize the treatment process for pulp diseases and explore future research directions and technological advancements.

准确评估牙髓状况对制定治疗策略至关重要。传统的评估方法包括主观症状、临床检查和影像学表现,尽管其诊断准确性存在争议。近年来,牙髓血流检测、牙髓炎症生物标志物、人工智能辅助诊断等新技术的引入,为牙髓疾病提供了更加客观、精确的诊断工具。本文系统地回顾了现有的牙髓状态评估方法,探讨了其临床诊断的有效性和局限性,并对现有的牙髓炎诊断系统进行了综述,以优化牙髓疾病的治疗过程,探索未来的研究方向和技术进步。
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引用次数: 0
[Preparation of nano BaTiO3@Au Schottky junction coatings on titanium implant and the influence on osteogenic properties of rat bone marrow stem cells]. 纳米BaTiO3@Au Schottky结膜在钛种植体上的制备及成骨性能评价
Q4 Medicine Pub Date : 2025-11-09 DOI: 10.3760/cma.j.cn112144-20250702-00242
X L Guo, D H Sun, L Y Li, L F Zhong, X Y Wang, Q Zhou, L Z Zhao
<p><p><b>Objective:</b> To prepare a nano-barium titanate@gold Schottky junction (nano-BaTiO<sub>3</sub>@Au) coating and investigate its effects on the adhesion, proliferation, and osteogenic differentiation of bone marrow stem cells (BMSCs), aiming to explore a titanium surface modification strategy with superior osteogenic activity. <b>Methods:</b> Pure titanium specimens served as the control group (Ti group). Titanium dioxide coatings were prepared on their surfaces via anodic oxidation. Nano-barium titanate (nBTO group) was further synthesized using the hydrothermal method. Gold nanoparticles were grown in situ on the nano-BaTiO<sub>3</sub> via high-temperature reduction of chloroauric acid using sodium citrate, yielding the nano-barium titanate@gold Schottky junction coating (nBTO@Au group). Surface morphology was observed by scanning electron microscopy (SEM). Elemental composition was analyzed using X-ray energy dispersive spectrum (EDS) and X-ray photoelectron spectroscopy (XPS). Crystal structure was analyzed using X-ray diffraction (XRD) and Raman spectroscopy. Hydrophilicity was assessed via water contact angle measurement. Specimens were co-cultured with BMSCs to evaluate biocompatibility and osteogenic properties. Cell proliferation on days 1, 3, 5, and 7 was assessed using the cell counting kit-8 (CCK-8) assay. Cytotoxicity towards BMSCs was assessed using live/dead cell staining. Cell morphology and adhesion were observed using cytoskeleton staining. Alkaline phosphatase (ALP) expression in BMSCs after 7 days was quantified using an ALP activity assay and ALP staining. Extracellular matrix mineralization after 7 days was evaluated using alizarin red staining and quantification assay. Each experiment was performed using three specimens per group. <b>Results:</b> Scanning electron microscopy revealed that gold nanoparticles with the diameter of(14.838±0.718) nm, uniform in size and homogeneously distributed, were successfully grown in situ on the surface of the nBTO coating. EDS and XPS confirmed the presence of Ba, Ti, O, and Au elements in the nBTO@Au composite coating. XRD and Raman spectroscopy analysis indicated that the nanostructured barium titanate (nBTO) coating was synthesized via a hydrothermal method.Water contact angle measurements showed that the contact angle was 66.8°± 0.45° for the control group, 22.55°±0.42° for the nBTO group, and 26.78°±1.15° for the nBTO@Au group, indicating good hydrophilicity of both nBTO and nBTO@Au coatings. On day 1 and day 3 of culture, the cell proliferation in the nBTO group was significantly lower than that in the control group (<i>P</i><0.05). In contrast, no significant differences were observed between the nBTO@Au group and either the control group or the nBTO group (all <i>P</i>>0.05). By day 5, the cell proliferation of nBTO@Au groups was significantly lower than that of the control group (<i>P</i><0.05), and the cell proliferation of nBTO group was significantly lower than that of
目的:制备纳米钡titanate@gold Schottky结(nano-BaTiO3@Au)涂层,研究其对骨髓干细胞(BMSCs)粘附、增殖和成骨分化的影响,探索具有优异成骨活性的钛表面修饰策略。方法:以纯钛标本为对照组(钛组)。通过阳极氧化在其表面制备了二氧化钛涂层。采用水热法进一步合成纳米钛酸钡(nBTO基团)。利用柠檬酸钠高温还原氯金酸,在纳米batio3上原位生长金纳米粒子,得到纳米钡titanate@gold肖特基结涂层(nBTO@Au基团)。用扫描电镜(SEM)观察表面形貌。采用x射线能谱(EDS)和x射线光电子能谱(XPS)分析元素组成。利用x射线衍射(XRD)和拉曼光谱分析了晶体结构。通过水接触角测定亲水性。将标本与骨髓间充质干细胞共培养,评价其生物相容性和成骨性能。使用细胞计数试剂盒-8 (CCK-8)测定第1、3、5和7天的细胞增殖情况。使用活/死细胞染色评估对骨髓间充质干细胞的细胞毒性。细胞骨架染色观察细胞形态和粘附情况。采用碱性磷酸酶(ALP)活性测定和ALP染色法测定7天后骨髓间充质干细胞中碱性磷酸酶(ALP)的表达。7 d后用茜素红染色和定量分析评价细胞外基质矿化。每组取3只标本。结果:扫描电镜显示,在nBTO涂层表面原位成功生长出平均直径约15.36 nm的金纳米颗粒,粒径均匀,分布均匀。EDS和XPS证实了nBTO@Au复合涂层中存在Ba、Ti、O和Au元素。XRD和拉曼光谱分析表明,采用水热法制备了纳米钛酸钡(nBTO)涂层。水接触角测量结果表明,对照组的接触角为66.8°±0.45°,nBTO组的接触角为22.55°±0.42°,nBTO@Au组的接触角为26.78°±1.15°,表明nBTO和nBTO@Au涂层均具有良好的亲水性。在培养第1天和第3天,nBTO组细胞增殖显著低于对照组(P0.05)。相比之下,nBTO@Au组与对照组或nBTO组之间均无显著差异(P < 0.05)。第5天,nBTO@Au组细胞增殖能力显著低于对照组(P0.05), nBTO组细胞增殖能力显著低于对照组和nBTO@Au组(P0.05)。第7天,各组细胞增殖能力比较,差异均无统计学意义(F=1.62, P < 0.05)。活/死细胞染色结果显示,各组细胞存活率均超过90%,形态正常,死亡细胞少,表明nBTO@Au涂层具有良好的生物相容性。与对照组相比,nBTO组和nBTO@Au组均促进了细胞的粘附和扩散,但两组间细胞形态无显著差异。ALP染色显示nBTO@Au组染色面积更大,颜色更深。定量结果显示nBTO@Au组ALP活性显著高于nBTO组和对照组(P0.05), nBTO组ALP活性也显著高于对照组(P0.05)。茜素红染色显示nBTO@Au组颜色最深,nBTO组次之,对照组颜色最浅。定量分析进一步证实nBTO@Au组钙结节沉积量显著大于其他两组(P0.05), nBTO组钙结节沉积量也显著大于对照组(P0.05)。结论:本研究成功制备了具有良好生物相容性和增强成骨性能的nBTO@Au涂层。
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引用次数: 0
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中华口腔医学杂志
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