Hamed Salehi, Stefan Reiser, Mohammad Pourkazemi, U. Focken
Iran, as one of the largest rainbow trout producers in the world, needs sustainable aquafeed resources to fulfill the requirements of this growing industry. Therefore, locally available canola meal, feather meal, blood meal and poultry by-product meal were evaluated on their suitability for feeding rainbow trout. Fish growth performance and apparent digestibility coefficients (ADCs) of nutrients were investigated for three casein-based fish meal-free diets, a practical feed including 10% spray-dried blood meal, 22% poultry by-product meal, 10% feather meal (GOLDMEHL®), 10% canola meal and 15.5% wheat flour in comparison to a commercial diet containing fish meal. The diets were allocated to aquaria in three replicates arranged in a random-block-design. Findings of the present study illustrated that growth performance remained unchanged (p>0.05) among fish fed fish meal-free diets and the commercial one. Formulating aquafeed using properly processed local feed ingredients such as poultry slaughterhouse by-products, canola meal, and crystalline amino acids can provide a sustainable solution to meet the feed requirements of the growing aquaculture industry on a regional scale. This research shows the potential for using locally available resources in aquafeed manufacturing.
{"title":"Complete Replacement of Fish Meal with Potential Aquafeed Ingredients for Rainbow Trout in Iran","authors":"Hamed Salehi, Stefan Reiser, Mohammad Pourkazemi, U. Focken","doi":"10.4194/trjfas24254","DOIUrl":"https://doi.org/10.4194/trjfas24254","url":null,"abstract":"Iran, as one of the largest rainbow trout producers in the world, needs sustainable aquafeed resources to fulfill the requirements of this growing industry. Therefore, locally available canola meal, feather meal, blood meal and poultry by-product meal were evaluated on their suitability for feeding rainbow trout. Fish growth performance and apparent digestibility coefficients (ADCs) of nutrients were investigated for three casein-based fish meal-free diets, a practical feed including 10% spray-dried blood meal, 22% poultry by-product meal, 10% feather meal (GOLDMEHL®), 10% canola meal and 15.5% wheat flour in comparison to a commercial diet containing fish meal. The diets were allocated to aquaria in three replicates arranged in a random-block-design. Findings of the present study illustrated that growth performance remained unchanged (p>0.05) among fish fed fish meal-free diets and the commercial one. Formulating aquafeed using properly processed local feed ingredients such as poultry slaughterhouse by-products, canola meal, and crystalline amino acids can provide a sustainable solution to meet the feed requirements of the growing aquaculture industry on a regional scale. This research shows the potential for using locally available resources in aquafeed manufacturing.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"36 12","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139167195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vikas Kumar, R. J. Shakila, A. U. Muzaddadi, G. Jeyasekaran, D. Sukumar, P. Padmavathy
Angiotensin-I converting enzyme inhibitory (ACEi) peptides were prepared from rohu (Labeo rohita) fish wastes by enzymatic hydrolysis [1.1% alcalase concentration (v/w); 130 min time; 52°C temperature and 0.8:1 solid-liquid ratio]. Ultrasound Assisted Enzymatic Extraction (UAEE) and Microwave Assisted Enzymatic Extraction (MAEE) were performed to enhance degree of hydrolysis (DH), ACEi activity, and peptide yield (PY). The ACEi peptides were examined for stability during thermal processing, varied pH treatments and in vitro gastrointestinal digestion. Peptides were stable at different temperatures (25, 37, 55, 75, and 100°C) except at 121°C which reduced their stability by 7%, 6.9% and 2.2% in UAEE, MAEE and Non-Assisted Enzymatic Extraction (NAEE) peptides, respectively. Similarly, peptides retained ACEi at pH 2 to 8 and subsequent reduction at pH>8. In vitro digestion studies showed stability of all peptides to GI digestion at 0.05% pepsin, 0.05% trypsin, 0.05% chymotrypsin and the combination of 0.025% trypsin and 0.025% chymotrypsin except MAEE peptides showing less stability with pepsin (0.05%). Therefore, it is concluded that these ACEi peptides derived from rohu fish waste, stable to heat, pH and gastrointestinal enzymatic action, can be used as ingredient in functional foods, which undergo thermal processing and digestion.
{"title":"In Vitro Stability of ACE-Inhibitory Peptides of Rohu Fish Waste to Heat, pH and Gastrointestinal Enzymes","authors":"Vikas Kumar, R. J. Shakila, A. U. Muzaddadi, G. Jeyasekaran, D. Sukumar, P. Padmavathy","doi":"10.4194/trjfas23788","DOIUrl":"https://doi.org/10.4194/trjfas23788","url":null,"abstract":"Angiotensin-I converting enzyme inhibitory (ACEi) peptides were prepared from rohu (Labeo rohita) fish wastes by enzymatic hydrolysis [1.1% alcalase concentration (v/w); 130 min time; 52°C temperature and 0.8:1 solid-liquid ratio]. Ultrasound Assisted Enzymatic Extraction (UAEE) and Microwave Assisted Enzymatic Extraction (MAEE) were performed to enhance degree of hydrolysis (DH), ACEi activity, and peptide yield (PY). The ACEi peptides were examined for stability during thermal processing, varied pH treatments and in vitro gastrointestinal digestion. Peptides were stable at different temperatures (25, 37, 55, 75, and 100°C) except at 121°C which reduced their stability by 7%, 6.9% and 2.2% in UAEE, MAEE and Non-Assisted Enzymatic Extraction (NAEE) peptides, respectively. Similarly, peptides retained ACEi at pH 2 to 8 and subsequent reduction at pH>8. In vitro digestion studies showed stability of all peptides to GI digestion at 0.05% pepsin, 0.05% trypsin, 0.05% chymotrypsin and the combination of 0.025% trypsin and 0.025% chymotrypsin except MAEE peptides showing less stability with pepsin (0.05%). Therefore, it is concluded that these ACEi peptides derived from rohu fish waste, stable to heat, pH and gastrointestinal enzymatic action, can be used as ingredient in functional foods, which undergo thermal processing and digestion.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"226 ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Capoeta kosswigi (Karaman, 1969) and Unio stevenianus (Krynicki, 1837) are endemic species in the Karasu river, the Lake Van basin. This study was carried out to determine the biological characteristics of C. kosswigi and distribution and prevalence of U. stevenianus glochidia on this host fish. A total of 379 C. kosswigi were caught by electrofishing between March 2022 and February 2023. The maximum fork length and total weights of C. kosswigi were 29.2 cm and 421.8 g for females and 27.8 cm and 337.9 g for males. The length-weight relationships were calculated as W=0.017L2.881 (r2=0.986) for males (negative allometric growth) and W=0.016L3.001 (r2=0.953) for females (isometric growth). The Fulton condition factor was determined as 1.53±0.13 (1.23-1.99). The female:male ratio was calculated as 0.18:1.00. Additionally, a total of 12525 Unionid glochidia were detected on C. kosswigi samples in this study. Glochidia was more inclined to prefer male C. kosswigi as a host. The highest amount of glochidia was recorded in May, while the lowest in August. The amount of glochidia was statistically found the highest in the gill filaments (454.3±678.0) compared to all fins. Therefore, the native species, C. kosswigi is a suitable host for the freshwater mussel, U. stevenianus.
Capoeta kosswigi（Karaman，1969 年）和 Unio stevenianus（Krynicki，1837 年）是凡湖流域卡拉苏河的特有物种。本研究旨在确定 C. kosswigi 的生物特征以及 U. stevenianus 珠虫在该宿主鱼类上的分布和流行情况。2022 年 3 月至 2023 年 2 月期间，通过电鱼共捕获了 379 条 C. kosswigi。雌鱼的最大叉长和总重分别为29.2厘米和421.8克，雄鱼的最大叉长和总重分别为27.8厘米和337.9克。经计算，雄鱼的体长-体重关系为 W=0.017L2.881 (r2=0.986) （负异速生长），雌鱼的体长-体重关系为 W=0.016L3.001 (r2=0.953) （等速生长）。富尔顿条件因子确定为 1.53±0.13 (1.23-1.99)。雌雄比例为 0.18:1.00。此外，本研究在 C. kosswigi 样品上共检测到 12525 个 Unionid 球虫。钩毛虫更倾向于以雄性 C. kosswigi 为宿主。五月份的钩毛虫数量最多，八月份最少。据统计，鳃丝中的钩毛数量（454.3±678.0）比所有鳍中的最高。因此，本地物种 C. kosswigi 是淡水贻贝 U. stevenianus 的合适宿主。
{"title":"Biological Characteristics of Siraz Fish, Capoeta kosswigi (Teleostei: Cyprinidae) and Host Relationship with Ectoparasitic Glochidia Larvae of Freshwater Mussel, Unio stevenianus (Bivalvia: Unionidae) in the Karasu River (Van, Türkiye)","authors":"A. Atıcı, Ertuğrul Kankaya","doi":"10.4194/trjfas24692","DOIUrl":"https://doi.org/10.4194/trjfas24692","url":null,"abstract":"Capoeta kosswigi (Karaman, 1969) and Unio stevenianus (Krynicki, 1837) are endemic species in the Karasu river, the Lake Van basin. This study was carried out to determine the biological characteristics of C. kosswigi and distribution and prevalence of U. stevenianus glochidia on this host fish. A total of 379 C. kosswigi were caught by electrofishing between March 2022 and February 2023. The maximum fork length and total weights of C. kosswigi were 29.2 cm and 421.8 g for females and 27.8 cm and 337.9 g for males. The length-weight relationships were calculated as W=0.017L2.881 (r2=0.986) for males (negative allometric growth) and W=0.016L3.001 (r2=0.953) for females (isometric growth). The Fulton condition factor was determined as 1.53±0.13 (1.23-1.99). The female:male ratio was calculated as 0.18:1.00. Additionally, a total of 12525 Unionid glochidia were detected on C. kosswigi samples in this study. Glochidia was more inclined to prefer male C. kosswigi as a host. The highest amount of glochidia was recorded in May, while the lowest in August. The amount of glochidia was statistically found the highest in the gill filaments (454.3±678.0) compared to all fins. Therefore, the native species, C. kosswigi is a suitable host for the freshwater mussel, U. stevenianus.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"183 ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Atlantic bonito (Sarda sarda) and Black Sea anchovy (Engraulis encrasicolus) fisheries in the southern part of the Black Sea currently lack a consistent harvesting strategy. To address this issue, a fishery model has been developed to optimize and stabilize the predator-prey relationship between these two species. This study offers an optimal and stable predator-prey relationship, resulting in higher landings and profits for the commercial fishery compared to the current harvesting strategy outlined in the study. The findings of this research can be utilized to determine sustainable yields and fishing quotas for these fisheries. Additionally, the examination of the predator-prey relationship between these species has revealed that, on an annual average, 93,259 tonnes of anchovy are consumed by the Atlantic bonito, which corresponds to approximately 46% of the estimated average anchovy landing. Furthermore, it has been observed that a 10% increase or decrease in the Atlantic bonito's feeding habits on the anchovy population leads to fluctuations in anchovy landings of up to 13% and anchovy profits of up to 40%.
{"title":"Predator Effect of Atlantic Bonito on the Black Sea Anchovy and Their Sustainable and Optimal Fishery","authors":"M. Demir","doi":"10.4194/trjfas23861","DOIUrl":"https://doi.org/10.4194/trjfas23861","url":null,"abstract":"The Atlantic bonito (Sarda sarda) and Black Sea anchovy (Engraulis encrasicolus) fisheries in the southern part of the Black Sea currently lack a consistent harvesting strategy. To address this issue, a fishery model has been developed to optimize and stabilize the predator-prey relationship between these two species. This study offers an optimal and stable predator-prey relationship, resulting in higher landings and profits for the commercial fishery compared to the current harvesting strategy outlined in the study. The findings of this research can be utilized to determine sustainable yields and fishing quotas for these fisheries. Additionally, the examination of the predator-prey relationship between these species has revealed that, on an annual average, 93,259 tonnes of anchovy are consumed by the Atlantic bonito, which corresponds to approximately 46% of the estimated average anchovy landing. Furthermore, it has been observed that a 10% increase or decrease in the Atlantic bonito's feeding habits on the anchovy population leads to fluctuations in anchovy landings of up to 13% and anchovy profits of up to 40%.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"497 ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Murniasih, Nur Agustin Mardiana, F. Untari, Joko Triwibowo, Asep Bayu
In our endeavor to find antibacterial from marine bacteria, we screened the microbiota from a Lithistid sponge, collected from Seribu Island, Jakarta. One of the promising strains was K 2.4.2. Identification of the strain using 16S rRNA Sanger sequencing resulted a sequence similarity of 100% to Bacillus tequilensis. Response surface methodology was applied to optimize the culture conditions for enhancing antibacterial activity of the strain. The optimization was carried out using Box-Behnken design experiment to determine the optimum carbon and nitrogen sources in order to produce maximum antibacterial activity. The optimum media with high antibacterial activity was marine broth with the addition of carbon and nitrogen concentration source of sucrose 2.15 % and peptone 1.08%, respectively. In addition, the optimum temperature was 31.18oC. The antibacterial activity response was predicted using a statistical model and compared to the activity in the experiment. The difference between the model and the laboratory experiment was less than 5% which showed a good accuracy. The results of this study once again showed that optimization method effectively reduced time, chemicals, and energy.
{"title":"Optimization of Carbon and Nitrogen Source to Enhance Antibacterial Activity from a Sponge-derived Bacillus tequilensis","authors":"T. Murniasih, Nur Agustin Mardiana, F. Untari, Joko Triwibowo, Asep Bayu","doi":"10.4194/trjfas24222","DOIUrl":"https://doi.org/10.4194/trjfas24222","url":null,"abstract":"In our endeavor to find antibacterial from marine bacteria, we screened the microbiota from a Lithistid sponge, collected from Seribu Island, Jakarta. One of the promising strains was K 2.4.2. Identification of the strain using 16S rRNA Sanger sequencing resulted a sequence similarity of 100% to Bacillus tequilensis. Response surface methodology was applied to optimize the culture conditions for enhancing antibacterial activity of the strain. The optimization was carried out using Box-Behnken design experiment to determine the optimum carbon and nitrogen sources in order to produce maximum antibacterial activity. The optimum media with high antibacterial activity was marine broth with the addition of carbon and nitrogen concentration source of sucrose 2.15 % and peptone 1.08%, respectively. In addition, the optimum temperature was 31.18oC. The antibacterial activity response was predicted using a statistical model and compared to the activity in the experiment. The difference between the model and the laboratory experiment was less than 5% which showed a good accuracy. The results of this study once again showed that optimization method effectively reduced time, chemicals, and energy.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"48 ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chae-Yeon Jeon, B. Seo, Josel Cadangin, Ji-Hye Lee, Su-Jin Park, Hsu Htoo, Ji-Sung Moon, Seunghan Lee, Sang-woo Hur, J. Song, Jae-Sik Kim, Kyeong-Jun Lee, Y. Choi
A six-month feeding trial was conducted to investigate the effect of moist pellet (MP) and commercial extruded pellet (EP) on the somatic growth, level and expression of GH/IGF axis, intestinal enzyme activity and gastrointestinal morphology of olive flounder (Paralichthys olivaceus) reared under actual farm conditions. MP was a raw fish-based diet composed of mackerel and cutlass fish while EP diet was a locally-produced commercial feed. Weight growth rate showed no significant difference (P>0.05), although EP-fed fish had a better somatic yield. Feed conversion ratio was better and condition factor has significantly improved when given EP diet (P<0.05). Plasma level of growth hormone and insulin-like growth factor 1 was comparably similar between diets, but EP-fed olive flounder had a higher hepatic IGF-1 expression (P<0.05). The activity of trypsin, chymotrypsin and lipase were insignificantly influenced by diets (P>0.05). The overall gastrointestinal morphology was in similitude regardless of diets, but EP- fed fish had a longer intestinal villi length and goblet cell count in the pyloric caeca (P<0.05). These dietary induced differences are discussed to have partly promoted better growth in EP diet. The findings suggest an equivalent, or better utilization of EP diet in the actual farm operation.
为了研究潮湿颗粒饲料（MP）和商品挤压颗粒饲料（EP）对实际养殖条件下饲养的橄榄鲽（Paralichthys olivaceus）的体细胞生长、GH/IGF 轴水平和表达、肠道酶活性和胃肠道形态的影响，进行了为期六个月的饲养试验。MP日粮是由鲭鱼和刀鱼组成的生鱼日粮，而EP日粮是当地生产的商品饲料。尽管 EP 饲料的鱼体产量更高，但体重增长率没有明显差异（P>0.05）。饲喂 EP 日粮时，饲料转化率更高，体况系数也明显改善（P0.05）。无论采用哪种日粮，总体胃肠道形态都相似，但 EP 饲喂的鱼肠绒毛长度更长，幽门盲肠中的腺细胞数量更多 (P<0.05)。据讨论，这些日粮引起的差异在一定程度上促进了 EP 日粮中鱼类更好的生长。研究结果表明，在实际养殖过程中，EP 日粮的利用率与 EP 日粮相当，甚至更高。
{"title":"Raw Fish-based Moist Pellet and Extruded Pellet on Growth, Levels and Expression of GH/IGF Axis, Intestinal Enzyme Activity and Gastrointestinal Morphology of Olive Flounder (Paralichthys olivaceus): An Actual Field Study","authors":"Chae-Yeon Jeon, B. Seo, Josel Cadangin, Ji-Hye Lee, Su-Jin Park, Hsu Htoo, Ji-Sung Moon, Seunghan Lee, Sang-woo Hur, J. Song, Jae-Sik Kim, Kyeong-Jun Lee, Y. Choi","doi":"10.4194/trjfas24397","DOIUrl":"https://doi.org/10.4194/trjfas24397","url":null,"abstract":"A six-month feeding trial was conducted to investigate the effect of moist pellet (MP) and commercial extruded pellet (EP) on the somatic growth, level and expression of GH/IGF axis, intestinal enzyme activity and gastrointestinal morphology of olive flounder (Paralichthys olivaceus) reared under actual farm conditions. MP was a raw fish-based diet composed of mackerel and cutlass fish while EP diet was a locally-produced commercial feed. Weight growth rate showed no significant difference (P>0.05), although EP-fed fish had a better somatic yield. Feed conversion ratio was better and condition factor has significantly improved when given EP diet (P<0.05). Plasma level of growth hormone and insulin-like growth factor 1 was comparably similar between diets, but EP-fed olive flounder had a higher hepatic IGF-1 expression (P<0.05). The activity of trypsin, chymotrypsin and lipase were insignificantly influenced by diets (P>0.05). The overall gastrointestinal morphology was in similitude regardless of diets, but EP- fed fish had a longer intestinal villi length and goblet cell count in the pyloric caeca (P<0.05). These dietary induced differences are discussed to have partly promoted better growth in EP diet. The findings suggest an equivalent, or better utilization of EP diet in the actual farm operation.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"463 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139282087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huaiyu Chen, Le Dong, MengTing Wang, Yongzhong Lu, Fang Wang
White spot syndrome virus (WSSV) has long been a major threat on shrimp industry, but there is always a lack of effective prevention and control methods. Vaccines are widely regarded as the safest and most effective methods for preventing WSSV, and some main envelope proteins have showed this potential. In order to combine the antigenicity of these proteins together for better vaccine performance, in this study a strategy commonly used in vertebrates was tried in shrimp. The dominant epitopes of five envelope proteins VP19, VP24, VP26, VP28 and VP41A were predicted and combined for tandem epitope vaccine design by bioinformatics methods, the designed vaccine gene was chemically synthesized and expressed in Escherichia coli, then the recombinant peptide was purified for function evaluation in shrimp. The results showed that the designed peptide had good flexibility, hydrophily and antigenicity with all the epitope regions accessible; the synthesized gene was linked with the tag sequence and successfully expressed in BL21(DE3), and the purified fusion peptide showed a positive effect in WSSV prevention. This research provided a promising method to design vaccines against WSSV, and further work in genetic engineering should be done so as to make it operable for application in shrimp farm.
{"title":"Design of Tandem Epitope Vaccine Against White Spot Syndrome Virus","authors":"Huaiyu Chen, Le Dong, MengTing Wang, Yongzhong Lu, Fang Wang","doi":"10.4194/trjfas24318","DOIUrl":"https://doi.org/10.4194/trjfas24318","url":null,"abstract":"White spot syndrome virus (WSSV) has long been a major threat on shrimp industry, but there is always a lack of effective prevention and control methods. Vaccines are widely regarded as the safest and most effective methods for preventing WSSV, and some main envelope proteins have showed this potential. In order to combine the antigenicity of these proteins together for better vaccine performance, in this study a strategy commonly used in vertebrates was tried in shrimp. The dominant epitopes of five envelope proteins VP19, VP24, VP26, VP28 and VP41A were predicted and combined for tandem epitope vaccine design by bioinformatics methods, the designed vaccine gene was chemically synthesized and expressed in Escherichia coli, then the recombinant peptide was purified for function evaluation in shrimp. The results showed that the designed peptide had good flexibility, hydrophily and antigenicity with all the epitope regions accessible; the synthesized gene was linked with the tag sequence and successfully expressed in BL21(DE3), and the purified fusion peptide showed a positive effect in WSSV prevention. This research provided a promising method to design vaccines against WSSV, and further work in genetic engineering should be done so as to make it operable for application in shrimp farm.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"9 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136157271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to investigate heavy metal accumulation in water-sediment, plankton, and macrophyte samples of Lake Iznik, one of the important lakes of Turkey. In 2016, water-sediment and plankton samples were taken from 19 and 10 stations respectively. In addition, heavy metal analyzes were performed on samples taken from Potamogeton pectinatus, Najas marina, Myriophyllum spicatum, Typha latifolia, Schoenoplectus litoralis, Phragmites australis, which are the most abundant macrophytes in the lake. The data of water and sediment samples were spatially analyzed in GIS and mapped with ArcGIS. In the water samples of Lake Iznik, arsenic (As), mercury (Hg) and in sediment samples, arsenic (As), chromium (Cr), copper (Cu) and nickel (Ni) were detected at remarkable levels. The results revealed that Iznik Lake was under pressure in terms of heavy metals and their effects should be reduced.
{"title":"Heavy Metal Accumulation in Aquatic Macrophytes and Plankton, Water-Sediment in Lake Iznik","authors":"Cafer Bulut","doi":"10.4194/trjfas24469","DOIUrl":"https://doi.org/10.4194/trjfas24469","url":null,"abstract":"This study aims to investigate heavy metal accumulation in water-sediment, plankton, and macrophyte samples of Lake Iznik, one of the important lakes of Turkey. In 2016, water-sediment and plankton samples were taken from 19 and 10 stations respectively. In addition, heavy metal analyzes were performed on samples taken from Potamogeton pectinatus, Najas marina, Myriophyllum spicatum, Typha latifolia, Schoenoplectus litoralis, Phragmites australis, which are the most abundant macrophytes in the lake. The data of water and sediment samples were spatially analyzed in GIS and mapped with ArcGIS. In the water samples of Lake Iznik, arsenic (As), mercury (Hg) and in sediment samples, arsenic (As), chromium (Cr), copper (Cu) and nickel (Ni) were detected at remarkable levels. The results revealed that Iznik Lake was under pressure in terms of heavy metals and their effects should be reduced.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"307 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136135602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cuneyt Kaya, Irmak Kurtul, Esra Baycelebi, Ali Ilhan, Hasan Musa Sari
The genus Petroleuciscus was known from the Marmara, Aegean, and Black Sea drainages, as well as southeast Anatolia and Iran. However, studies conducted in recent years have suggested that the species described in southeast Anatolia and Iran belong to different genera. The results and recommendations of these studies were evaluated. On the other hand, in the scope of this study, the taxonomic status and length-weight relationship of several Petroleuciscus ninae populations in Western Anatolia were evaluated. The morphological comparisons revealed that Tahtalı Reservoir, Küçük Menderes and Sarıçay rivers, and Acıgöl Lake`s Petroleuciscus populations recorded as Petroleuciscus smyrnaeus in all previous studies belong to P. ninae which was known only from the type locality Akçay Stream. In addition, the length-weight relationship of P. ninae in the lakes Akgöl and Belevi, Lake Acıgöl, stream Akçay, Sarıçay River, an inflow of Yenişehir Reservoir and inflow of Tahtalı Reservoir were studied. The following research considers some first records and comprehensive information on the length-weight relationship of P. ninae in the Western Anatolia water resources. In study, the constant b changed from 3.101 to 3.389 (Akçay Stream) in all the sampling locations. It is expected that the results of this research might support the conservation of this species and contribute knowledge of its taxonomic status.
石油鱼属产于马尔马拉、爱琴海和黑海流域，以及安纳托利亚东南部和伊朗。然而，近年来进行的研究表明，在安纳托利亚东南部和伊朗描述的物种属于不同的属。对这些研究的结果和建议进行了评价。另一方面，在本研究的范围内，评价了西部安纳托利亚几个石油石楠种群的分类地位和长度-权重关系。形态比较表明，塔塔尔卡水库、k k Menderes河和Sarıçay河，以及Acıgöl湖的石油鱼种群均记录为石油鱼smyrnaeus，属于P. ninae，仅在类型地akay溪中发现。此外，还研究了ninae在Akgöl湖、Belevi湖、Acıgöl湖、akay河、Sarıçay河、yeni ehir水库入流和tahtalbi水库入流中的长度-权重关系。下面的研究考虑了西部安纳托利亚水资源中关于九头水母长度-权重关系的一些第一手记录和综合资料。研究中，各采样点的常数b在3.101 ~ 3.389 (akay Stream)之间变化。期望本研究结果能为该物种的保护提供支持，并有助于了解其分类学地位。
{"title":"Actual Distributions and Validity of Petroleuciscus spp., with the Range Extension and Length-Weight Relationship Data in Case of Petroleuciscus ninae","authors":"Cuneyt Kaya, Irmak Kurtul, Esra Baycelebi, Ali Ilhan, Hasan Musa Sari","doi":"10.4194/trjfas23846","DOIUrl":"https://doi.org/10.4194/trjfas23846","url":null,"abstract":"The genus Petroleuciscus was known from the Marmara, Aegean, and Black Sea drainages, as well as southeast Anatolia and Iran. However, studies conducted in recent years have suggested that the species described in southeast Anatolia and Iran belong to different genera. The results and recommendations of these studies were evaluated. On the other hand, in the scope of this study, the taxonomic status and length-weight relationship of several Petroleuciscus ninae populations in Western Anatolia were evaluated. The morphological comparisons revealed that Tahtalı Reservoir, Küçük Menderes and Sarıçay rivers, and Acıgöl Lake`s Petroleuciscus populations recorded as Petroleuciscus smyrnaeus in all previous studies belong to P. ninae which was known only from the type locality Akçay Stream. In addition, the length-weight relationship of P. ninae in the lakes Akgöl and Belevi, Lake Acıgöl, stream Akçay, Sarıçay River, an inflow of Yenişehir Reservoir and inflow of Tahtalı Reservoir were studied. The following research considers some first records and comprehensive information on the length-weight relationship of P. ninae in the Western Anatolia water resources. In study, the constant b changed from 3.101 to 3.389 (Akçay Stream) in all the sampling locations. It is expected that the results of this research might support the conservation of this species and contribute knowledge of its taxonomic status.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To assess the feasibility of incorporating insect meal and oil into diets for Pacific white shrimp Litopenaeus Vannamei, a trial was conducted to measure growth and feed performance metrics within balanced iso-nitrogenous and iso-lipid (36% protein and 6% lipid) experimental diets formulated with standard commercially sources ingredients. Incremental levels of BSF larval meal (BSFM, 0.5 to 5%) were used to partially substitute the use of fishmeal (FM) in the diet. In addition, next group of dietS were designed by using incremental levels of BSF larval oil (BSFO (0.5 to 5%) with 0.5% BSFM as a reference to partially and completely replace the use of fish oil (FO) within the diet. Ten dietary treatments were evaluated as four replicates in 40 Hapa ponds assigned randomly. Shrimp of initial mean weight of 0.97 g were fed by hand over 90 days with feed intake based on an FCR of 1.5 and all parameters recorded to determine response. Inclusion of up to 5% of both BSF meal and oil improved performance with respect to growth rate and feed utilization efficiency FCR. Total hemocyte counts and lysozyme activity reflected these trends displaying advantages of BSF diet groups compared to the basal fed group of L. Vannamei. After the growth trial, a disease challenge test was undertaken using an infection model with Vibrio harveyi at a concentration of 105 CFU shrimp-1 under controlled laboratory settings. As such, several indices of health status were recorded that included hemocyte counts, lysozyme activity as well as histopathology of the hepatopancreas that is a prime indicator of the progression of disease and a reflection on health status. Insect meal and oil inclusions increased survival from 40% to 60 - 80% and in accordance, raised the immune response and improved histopathological profiles of hepatopancreas tissues.
{"title":"Black Soldier Fly (Hermetia illucens) as an Alternative to Marine Ingredients Elicits Superior Growth Performance and Resistance to Vibrio harveyi Infection for Pacific White Shrimp (Litopenaeus vannamei)","authors":"Romi Novriadi, Simon Davies, Komang Indra Kurnia Triatmaja, Maman Hermawan, Endhay Kusnendar Muljana Kontara, Budi Tanaka, Ahmad Rinaldy, Jovano Erris Nugroho","doi":"10.4194/trjfas24343","DOIUrl":"https://doi.org/10.4194/trjfas24343","url":null,"abstract":"To assess the feasibility of incorporating insect meal and oil into diets for Pacific white shrimp Litopenaeus Vannamei, a trial was conducted to measure growth and feed performance metrics within balanced iso-nitrogenous and iso-lipid (36% protein and 6% lipid) experimental diets formulated with standard commercially sources ingredients. Incremental levels of BSF larval meal (BSFM, 0.5 to 5%) were used to partially substitute the use of fishmeal (FM) in the diet. In addition, next group of dietS were designed by using incremental levels of BSF larval oil (BSFO (0.5 to 5%) with 0.5% BSFM as a reference to partially and completely replace the use of fish oil (FO) within the diet. Ten dietary treatments were evaluated as four replicates in 40 Hapa ponds assigned randomly. Shrimp of initial mean weight of 0.97 g were fed by hand over 90 days with feed intake based on an FCR of 1.5 and all parameters recorded to determine response. Inclusion of up to 5% of both BSF meal and oil improved performance with respect to growth rate and feed utilization efficiency FCR. Total hemocyte counts and lysozyme activity reflected these trends displaying advantages of BSF diet groups compared to the basal fed group of L. Vannamei. After the growth trial, a disease challenge test was undertaken using an infection model with Vibrio harveyi at a concentration of 105 CFU shrimp-1 under controlled laboratory settings. As such, several indices of health status were recorded that included hemocyte counts, lysozyme activity as well as histopathology of the hepatopancreas that is a prime indicator of the progression of disease and a reflection on health status. Insect meal and oil inclusions increased survival from 40% to 60 - 80% and in accordance, raised the immune response and improved histopathological profiles of hepatopancreas tissues.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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