Three of six commercial spiny lobster species in Indonesian waters was found in Aru Islands waters which exploited continuously using drift gillnet and the catch was dominated by painted spiny lobster (Panulirus versicolor). To ensure the sustainability of the lobster stock, fisheries should be managed properly based on the best available science. In data-limited fisheries, length composition data are most common form information available for researchers and managers since its relatively cheap and simple to collect. In this study, we evaluate the stock status of P. versicolor in Aru Islands waters using three length-based methods: 1) length-converted catch curve (LCCC), 2) length-based spawning potential ratio (LB-SPR) and 3) length-based Bayesian biomass (LBB). The result showed that the mean length at catch of P. versicolor was 80.9 mm in carapace length. Furthermore, the three methods agree that the current status of painted spiny lobster stock seems to be fully exploited where the exploitation rate, the spawning potential ratio, and relative stock size (B/BMSY) were 54%, 35%, and 85%, respectively. Thus, for sustainability of this stock, it is highly recommended to catch P. versicolor at size greater than 80 mm in carapace length.
{"title":"Inferring Stock Status of Painted Spiny Lobster (Panulirus versicolor) in Aru Islands Waters, Indonesia","authors":"D. Kembaren, A. Suman","doi":"10.4194/trjfas21543","DOIUrl":"https://doi.org/10.4194/trjfas21543","url":null,"abstract":"Three of six commercial spiny lobster species in Indonesian waters was found in Aru Islands waters which exploited continuously using drift gillnet and the catch was dominated by painted spiny lobster (Panulirus versicolor). To ensure the sustainability of the lobster stock, fisheries should be managed properly based on the best available science. In data-limited fisheries, length composition data are most common form information available for researchers and managers since its relatively cheap and simple to collect. In this study, we evaluate the stock status of P. versicolor in Aru Islands waters using three length-based methods: 1) length-converted catch curve (LCCC), 2) length-based spawning potential ratio (LB-SPR) and 3) length-based Bayesian biomass (LBB). The result showed that the mean length at catch of P. versicolor was 80.9 mm in carapace length. Furthermore, the three methods agree that the current status of painted spiny lobster stock seems to be fully exploited where the exploitation rate, the spawning potential ratio, and relative stock size (B/BMSY) were 54%, 35%, and 85%, respectively. Thus, for sustainability of this stock, it is highly recommended to catch P. versicolor at size greater than 80 mm in carapace length.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45483045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Savas Kilic, S. Ozturk, Suleyman Sarigoz, S. Erkan, M. Kanyılmaz, E. Tıraşın, M. Vasconcellos, S. Lelli, N. Demirel
Stock assessment for Mullus barbatus using catch-based (CMSY) and length based (LBB, LBSPR and LIME) data limited models were performed by using official catch statistics and commercial length frequency data from northeastern Mediterranean. The CMSY model results showed that the catch amount did not exceed MSY, and the fisheries reference points (B/BMSY and F/FMSY) indicated a healthy stock status in 2020. The LBB model results indicated that the biomass ratio was above safe biological limits (B<0.5 BMSY) but fishing mortality was slightly higher than natural mortality. Likewise, LBSPR results showed that SL50 for red mullet was higher than its Lm50, while the estimated median SPR was above the limit of 20% set as the biological reference point. The LIME results were more optimistic than those from the LBB and LBSPR and the estimated SPR was found above 40% which is the target reference point for a sustainable stock. According to the results of different assessment models, the red mullet stock was deemed sustainable in terms of biomass but observation of fishing pressure is required.
{"title":"Stock Assessment of Red Mullet, Mullus barbatus Linnaeus, 1758 by Length-based and Catch-based Models in the Northeastern Mediterranean","authors":"Savas Kilic, S. Ozturk, Suleyman Sarigoz, S. Erkan, M. Kanyılmaz, E. Tıraşın, M. Vasconcellos, S. Lelli, N. Demirel","doi":"10.4194/trjfas22719","DOIUrl":"https://doi.org/10.4194/trjfas22719","url":null,"abstract":"Stock assessment for Mullus barbatus using catch-based (CMSY) and length based (LBB, LBSPR and LIME) data limited models were performed by using official catch statistics and commercial length frequency data from northeastern Mediterranean. The CMSY model results showed that the catch amount did not exceed MSY, and the fisheries reference points (B/BMSY and F/FMSY) indicated a healthy stock status in 2020. The LBB model results indicated that the biomass ratio was above safe biological limits (B<0.5 BMSY) but fishing mortality was slightly higher than natural mortality. Likewise, LBSPR results showed that SL50 for red mullet was higher than its Lm50, while the estimated median SPR was above the limit of 20% set as the biological reference point. The LIME results were more optimistic than those from the LBB and LBSPR and the estimated SPR was found above 40% which is the target reference point for a sustainable stock. According to the results of different assessment models, the red mullet stock was deemed sustainable in terms of biomass but observation of fishing pressure is required.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43232886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenneth Gabriel Mota, Jussara Oliveira Vaini, F. V. Velho, M. Sardinha, A. Hilsdorf
This study provides the first set of 20 species-specific microsatellite loci for genetic population studies of Trachurus trecae, an important marine fishing resource for the African Atlantic coast. Twenty microsatellite loci were developed by next generation sequencing and tested by genotyping 48 animals collected along the Angolan Atlantic coast. The number of alleles per locus varied from 6 to 19, while the expected and observed heterozygosities varied from 0.73 to 0.94 and 0.16 to 0.86, respectively. These microsatellites are a valuable tool for further genetics monitoring of this species.
{"title":"Twenty Polymorphic Microsatellite Markers for Trachurus trecae Cadenat, 1950 (Perciformes: Carangidae): An Important Fishing Genetic Resource Along the West African Atlantic Coast","authors":"Kenneth Gabriel Mota, Jussara Oliveira Vaini, F. V. Velho, M. Sardinha, A. Hilsdorf","doi":"10.4194/trjfas20982","DOIUrl":"https://doi.org/10.4194/trjfas20982","url":null,"abstract":"This study provides the first set of 20 species-specific microsatellite loci for genetic population studies of Trachurus trecae, an important marine fishing resource for the African Atlantic coast. Twenty microsatellite loci were developed by next generation sequencing and tested by genotyping 48 animals collected along the Angolan Atlantic coast. The number of alleles per locus varied from 6 to 19, while the expected and observed heterozygosities varied from 0.73 to 0.94 and 0.16 to 0.86, respectively. These microsatellites are a valuable tool for further genetics monitoring of this species.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42600950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Minaz, A. Er, K. Ak, Ikbal Demet Nane, Z. Ipek, R. Aslankoç
Used as a component of polycarbonate plastics and epoxy resins in the modern industrial world, bisphenol A (BPA) is one of the highest volume produced industrial chemicals worldwide. BPA causes important environmental problems including endocrine disrupting effect on the organisms in aquatic environments. This study focused on the histopathological, oxidative and hematological effects of long-term BPA exposure on wild vimba bream (Vimba vimba) for the first time. Twenty-five fish stocked in each aquarium and exposed to 1000 μg/L BPA for four weeks. Hematological parameters of fish did not differ significantly compared to control group. The antioxidant activity of liver and gill tissues created a significant partial difference between the groups. According to the histopathological studies, intense hyperplasia and necrosis were detected in the gill tissues of the fish exposed BPA. In addition, hypertrophy and epithelial lifting symptom were partly observed. Vocalization and hypertrophic cells was determined in the liver tissues. In conclusions, this study revealed that BPA had an oxidative and especially histopathologically adverse effects on V. vimba, even though it did not have a hematological effect on the treated fish. Although BPA has adverse effects on the health of aquatic organisms, future studies should focus on the residue in fish meat and risk assessment on human health.
{"title":"Bisphenol A Used in Plastic Industry Negatively Affects Wild Vimba Bream (Vimba vimba)","authors":"M. Minaz, A. Er, K. Ak, Ikbal Demet Nane, Z. Ipek, R. Aslankoç","doi":"10.4194/trjfas22598","DOIUrl":"https://doi.org/10.4194/trjfas22598","url":null,"abstract":"Used as a component of polycarbonate plastics and epoxy resins in the modern industrial world, bisphenol A (BPA) is one of the highest volume produced industrial chemicals worldwide. BPA causes important environmental problems including endocrine disrupting effect on the organisms in aquatic environments. This study focused on the histopathological, oxidative and hematological effects of long-term BPA exposure on wild vimba bream (Vimba vimba) for the first time. Twenty-five fish stocked in each aquarium and exposed to 1000 μg/L BPA for four weeks. Hematological parameters of fish did not differ significantly compared to control group. The antioxidant activity of liver and gill tissues created a significant partial difference between the groups. According to the histopathological studies, intense hyperplasia and necrosis were detected in the gill tissues of the fish exposed BPA. In addition, hypertrophy and epithelial lifting symptom were partly observed. Vocalization and hypertrophic cells was determined in the liver tissues. In conclusions, this study revealed that BPA had an oxidative and especially histopathologically adverse effects on V. vimba, even though it did not have a hematological effect on the treated fish. Although BPA has adverse effects on the health of aquatic organisms, future studies should focus on the residue in fish meat and risk assessment on human health.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45100046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the fast development of industries relevant to nanotechnology, the inappropriate disposal of nanoproducts may initiate a new source of pollution in aquatic ecosystems, thus posing a possible danger to aquatic life. This study evaluated the eco-toxicological effects of waterborne copper oxide nanoparticles (CuO-NPs) having a 32.84nm size and rod shape on a freshwater fish, Labeo rohita. 96-h LC50 of CuO-NPs was 353.98mg/L. Two sub-lethal concentrations equivalent to 1/3rd and 1/5th LC50/96h (70.79 and 117.99 mg/L) of CuO-NPs were selected for 15, 30, and 45-day exposure tests. Bioaccumulation for the 1/3rd 96h LC50 was significantly higher compared to 1/5th of 96-h LC50 of CuO-NPs. There was a sharp decrease in the CAT activity and this decline ultimately increased the TBARS contents. The highest percentage of damaged nuclei and genetic damage index in fish erythrocytes was recorded at the highest concentration and after 45 days of treatment. The adverse effects of CuO-NPs were examined to be dose and duration dependent with increasing extent during all studied time intervals. Summarizing, exposure to sublethal concentrations of CuO-NPs is sufficient to cause alterations in ecotoxicological endpoints such as metal overload, oxidative stress and genotoxicity after chronic exposure.
随着纳米技术相关产业的快速发展，纳米产品的不当处理可能会在水生生态系统中引发新的污染源，从而可能对水生生物造成危害。本研究评估了32.84nm大小和棒状的水性氧化铜纳米颗粒(CuO-NPs)对淡水鱼Labeo rohita的生态毒理学效应。CuO-NPs 96 h LC50为353.98mg/L。选择相当于1/3和1/5 LC50/96h(70.79和117.99 mg/L)的CuO-NPs两个亚致死浓度进行15、30和45天的暴露试验。CuO-NPs在96h LC50的1/3积累量显著高于96h LC50的1/5。CAT活性急剧下降，最终导致TBARS含量增加。在最高浓度和处理45 d后，鱼红细胞的细胞核损伤率和遗传损伤指数最高。在所有研究时间间隔内，CuO-NPs的不良反应均呈剂量和持续时间依赖性，且程度增加。综上所述，暴露于亚致死浓度的CuO-NPs足以引起生态毒理学终点的改变，如慢性暴露后的金属过载、氧化应激和遗传毒性。
{"title":"Evaluation of Toxicity Induced by Engineered CuO Nanoparticles in Freshwater Fish, Labeo rohita","authors":"S. Aziz, S. Abdullah","doi":"10.4194/trjfas18762","DOIUrl":"https://doi.org/10.4194/trjfas18762","url":null,"abstract":"With the fast development of industries relevant to nanotechnology, the inappropriate disposal of nanoproducts may initiate a new source of pollution in aquatic ecosystems, thus posing a possible danger to aquatic life. This study evaluated the eco-toxicological effects of waterborne copper oxide nanoparticles (CuO-NPs) having a 32.84nm size and rod shape on a freshwater fish, Labeo rohita. 96-h LC50 of CuO-NPs was 353.98mg/L. Two sub-lethal concentrations equivalent to 1/3rd and 1/5th LC50/96h (70.79 and 117.99 mg/L) of CuO-NPs were selected for 15, 30, and 45-day exposure tests. Bioaccumulation for the 1/3rd 96h LC50 was significantly higher compared to 1/5th of 96-h LC50 of CuO-NPs. There was a sharp decrease in the CAT activity and this decline ultimately increased the TBARS contents. The highest percentage of damaged nuclei and genetic damage index in fish erythrocytes was recorded at the highest concentration and after 45 days of treatment. The adverse effects of CuO-NPs were examined to be dose and duration dependent with increasing extent during all studied time intervals. Summarizing, exposure to sublethal concentrations of CuO-NPs is sufficient to cause alterations in ecotoxicological endpoints such as metal overload, oxidative stress and genotoxicity after chronic exposure.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47304971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The family Gobiidae constitutes one of the most diverse groups among teleost fish; some of its species are objects of aquaculture and fisheries. Despite many years of cytogenetic studies, the chromosome complements of many species of gobies remain unknown, and the trends of karyotypic evolution are poorly understood. Here I describe a previously unstudied karyotype of a widespread species from the group of sand gobies (Pomatoschistus marmoratus) and analyze the trends of karyotypic evolution of this group in comparison with other taxonomic groups of gobies from the Atlantic-Mediterranean and Ponto-Caspian regions. For P. marmoratus, the diploid number is 2n=46; it consists of 11 pairs of submetacentric chromosomes, 12 pairs of subtelocentric and telocentric chromosomes (NF=68). A comparative karyological analysis showed that the main trend in the karyotypic evolution of sand gobies differs from that of other groups of gobies and is associated with an increase in the number of chromosomal arms as a result of chromosomal rearrangements leading to a change in the centromere position. The results obtained contribute to understanding the role of chromosomal rearrangements in the evolution of Gobiidae and are of interest for further cytogenetic studies of this large family of fish.
{"title":"Chromosome Complement of Pomatoschistus marmoratus and Karyotype Evolution in Sand Gobies (Gobiidae: Gobionellinae)","authors":"D. V. Prazdnikov","doi":"10.4194/trjfas20729","DOIUrl":"https://doi.org/10.4194/trjfas20729","url":null,"abstract":"The family Gobiidae constitutes one of the most diverse groups among teleost fish; some of its species are objects of aquaculture and fisheries. Despite many years of cytogenetic studies, the chromosome complements of many species of gobies remain unknown, and the trends of karyotypic evolution are poorly understood. Here I describe a previously unstudied karyotype of a widespread species from the group of sand gobies (Pomatoschistus marmoratus) and analyze the trends of karyotypic evolution of this group in comparison with other taxonomic groups of gobies from the Atlantic-Mediterranean and Ponto-Caspian regions. For P. marmoratus, the diploid number is 2n=46; it consists of 11 pairs of submetacentric chromosomes, 12 pairs of subtelocentric and telocentric chromosomes (NF=68). A comparative karyological analysis showed that the main trend in the karyotypic evolution of sand gobies differs from that of other groups of gobies and is associated with an increase in the number of chromosomal arms as a result of chromosomal rearrangements leading to a change in the centromere position. The results obtained contribute to understanding the role of chromosomal rearrangements in the evolution of Gobiidae and are of interest for further cytogenetic studies of this large family of fish.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42318865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, Labeo rohita fingerlings were maintained either at ambient water temperature (26ºC) for five weeks or exposed to 32ºC for one week then later maintained at 26ºC for four weeks. Fingerlings reared under different temperature regimes were fed with any of the four experimental diets containing 20, 30, 40 or 45% protein. Serum cortisol level was higher at 26ºC compared to 32ºC, and decrease with the increase in dietary protein level up to 40%. Fingerlings fed 30% and 40% protein recorded similar WBCS count and respiratory burst activity which was lower and higher respectively compared to 20% and 45% protein fed fingerlings. Correspondingly, lower WBCS count and higher NBT were recorded following exposure to higher temperature (32ºC) for one week compared to 26ºC exposure group. Significantly lower survival was recorded in groups fed with lowest (20%) and highest (45%) dietary protein level whereas fingerlings exposed to 32ºC for one week exhibited higher survival (%) compare to 26ºC. Present results indicate that both lower and higher level of dietary protein may cause metabolic stress to fingerlings, as might consequently lead to the depressed immunity and exposure of elevated temperature (32ºC) for one week mitigates this immunosuppressive effect.
{"title":"Elevation in Rearing Temperature within Optimum Range Mitigate Immunosuppressive and Metabolic Stress Effect of High or Low Dietary Protein Level in Labeo Rohita Fingerlings","authors":"Shivendra Kumar, N. P. Sahu, S. Gupta","doi":"10.4194/trjfas19872","DOIUrl":"https://doi.org/10.4194/trjfas19872","url":null,"abstract":"In the present study, Labeo rohita fingerlings were maintained either at ambient water temperature (26ºC) for five weeks or exposed to 32ºC for one week then later maintained at 26ºC for four weeks. Fingerlings reared under different temperature regimes were fed with any of the four experimental diets containing 20, 30, 40 or 45% protein. Serum cortisol level was higher at 26ºC compared to 32ºC, and decrease with the increase in dietary protein level up to 40%. Fingerlings fed 30% and 40% protein recorded similar WBCS count and respiratory burst activity which was lower and higher respectively compared to 20% and 45% protein fed fingerlings. Correspondingly, lower WBCS count and higher NBT were recorded following exposure to higher temperature (32ºC) for one week compared to 26ºC exposure group. Significantly lower survival was recorded in groups fed with lowest (20%) and highest (45%) dietary protein level whereas fingerlings exposed to 32ºC for one week exhibited higher survival (%) compare to 26ºC. Present results indicate that both lower and higher level of dietary protein may cause metabolic stress to fingerlings, as might consequently lead to the depressed immunity and exposure of elevated temperature (32ºC) for one week mitigates this immunosuppressive effect.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43345385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neopyropia yezoensis is a type of red macroalgae that is becoming economically important owing to increasing demand. A high-growth-rate mutant, NyEMS, was recently developed using ethyl methanesulfonate to meet this demand. However, the high-growth-rate mechanisms of NyEMS have not yet been identified because of the poor understanding of N. yezoensis biomolecules. In this study, NyEMS was subjected to comparative transcriptome analysis using RNA-seq. As a result, total of 7,933 differentially expressed unigenes were identified. From the transcriptome analysis, it was demonstrated that increased proteasome expression and repressed 1- aminocyclopropane-l-carboxylate (ACC) production could be responsible for the enhanced growth rate of the mutant. Additionally, genes involved in vitamin B6 biosynthesis were up-regulated, while those related to phycobilisomes were downregulated in the mutant, which could be responsible for its characteristic features. Moreover, 1,281 SNPs and 12,963 cSSRs were identified from assembled data. To determine possible biomarkers, primer pairs were designed from the identified cDNAderived simple sequence repeats. This study expands our understanding of the genetic mechanisms of N. yezoensis for future research and will be helpful in the development of N. yezoensis biomarkers.
{"title":"Differential Gene Expression Analysis of Neopyropia yezoensis (Bangiales, Rhodophyta) Mutant with High-growth and Development of SSR Marker Using RNA-seq","authors":"SeoHwa Park, Jong-il Choi","doi":"10.4194/trjfas21732","DOIUrl":"https://doi.org/10.4194/trjfas21732","url":null,"abstract":"Neopyropia yezoensis is a type of red macroalgae that is becoming economically important owing to increasing demand. A high-growth-rate mutant, NyEMS, was recently developed using ethyl methanesulfonate to meet this demand. However, the high-growth-rate mechanisms of NyEMS have not yet been identified because of the poor understanding of N. yezoensis biomolecules. In this study, NyEMS was subjected to comparative transcriptome analysis using RNA-seq. As a result, total of 7,933 differentially expressed unigenes were identified. From the transcriptome analysis, it was demonstrated that increased proteasome expression and repressed 1- aminocyclopropane-l-carboxylate (ACC) production could be responsible for the enhanced growth rate of the mutant. Additionally, genes involved in vitamin B6 biosynthesis were up-regulated, while those related to phycobilisomes were downregulated in the mutant, which could be responsible for its characteristic features. Moreover, 1,281 SNPs and 12,963 cSSRs were identified from assembled data. To determine possible biomarkers, primer pairs were designed from the identified cDNAderived simple sequence repeats. This study expands our understanding of the genetic mechanisms of N. yezoensis for future research and will be helpful in the development of N. yezoensis biomarkers.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46735338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Bano, N. Khan, M. Arslan, M. Fatima, A. Khalique, A. Wan
The present study was conducted to evaluate the effect of different dietary protein levels on digestive enzyme activities, nutrient assimilation as well as growth performance in striped catfish (Pangasianodon hypophthalmus). Fish were fed with four semi-purified formulated diets with increasing levels of protein: 30% (CP30), 35% (CP35), 40% (CP40) and 45% (CP45) for 12 weeks. At the end of the feed trial, the highest weight gain (29.88 g fish-1), specific growth rate (3.58%) and lower feed conversion ratio (1.91) were observed in fish that were fed with a 40% protein diet while CP30 treatment group had the poorest growth performance indicators (P<0.05). Proximate analysis revealed that fish fed with either CP40 (18.50%) or CP45 (19.33%) diets had the highest protein content in comparison to that fed CP30 (17.13%) and CP35 (17.00%) diets. Similarly, the highest body lipid content was CP35 (4.20%) treatment group and the lowest in CP40 (3.83%). The amino acid in the harvested fillets was also significantly higher (P<0.05) in both CP40 and CP45 dietary treatments. The increasing dietary protein level also elevated both protease and amylase activity, while lipase activity significantly decreased (P<0.05). The study revealed that a 40 % protein level diet produced from regional ingredients in the Middle East had yielded optimal growth performance and higher muscle quality in striped catfish.
{"title":"Impact of Various Dietary Protein Levels on the Growth Performance, Nutrient Profile, and Digestive Enzymes Activities to Provide an Effective Diet for Striped Catfish (Pangasius Hypophthalmus)","authors":"S. Bano, N. Khan, M. Arslan, M. Fatima, A. Khalique, A. Wan","doi":"10.4194/trjfas22204","DOIUrl":"https://doi.org/10.4194/trjfas22204","url":null,"abstract":"The present study was conducted to evaluate the effect of different dietary protein levels on digestive enzyme activities, nutrient assimilation as well as growth performance in striped catfish (Pangasianodon hypophthalmus). Fish were fed with four semi-purified formulated diets with increasing levels of protein: 30% (CP30), 35% (CP35), 40% (CP40) and 45% (CP45) for 12 weeks. At the end of the feed trial, the highest weight gain (29.88 g fish-1), specific growth rate (3.58%) and lower feed conversion ratio (1.91) were observed in fish that were fed with a 40% protein diet while CP30 treatment group had the poorest growth performance indicators (P<0.05). Proximate analysis revealed that fish fed with either CP40 (18.50%) or CP45 (19.33%) diets had the highest protein content in comparison to that fed CP30 (17.13%) and CP35 (17.00%) diets. Similarly, the highest body lipid content was CP35 (4.20%) treatment group and the lowest in CP40 (3.83%). The amino acid in the harvested fillets was also significantly higher (P<0.05) in both CP40 and CP45 dietary treatments. The increasing dietary protein level also elevated both protease and amylase activity, while lipase activity significantly decreased (P<0.05). The study revealed that a 40 % protein level diet produced from regional ingredients in the Middle East had yielded optimal growth performance and higher muscle quality in striped catfish.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":"38 12","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41294809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Krupesha Sharma S.R., Sumithra T.G., R. P, A. K, Ambarish P. Gop, R. R., A. Joshy, V. Prasad, S. G, R. R., Panda S.K., T. G., Anil M.K., P. K. Patil
Trachinotus blochii is a promising mariculture fish species. Scientific data on biosafety and fillet residues of florfenicol exposure, one recommended amphenicol antimicrobial for aquaculture use, remains unknown in T. blochii, despite its criticality for prudent application. Accordingly, the paper evaluated the safety (regarding mortality, symptoms, weight gain, and histopathology) of dietary florfenicol after therapeutic (10 mg Kg-1 for ten days) and excessive (three, five, and ten times the therapeutic dose for 10, 20, and 30 days) exposures. There was no mortality in any group. The clinical abnormalities were noted only in 10X group from the 25th exposure day, which disappeared on the fourth day after withdrawal. Reduced growth was recorded at 5X and 10X groups from 20 and 30 exposure days, respectively. Histological lesion`s severity was in the liver > kidney > gill > spleen > muscle > intestine. The lesion severity relied on the quantity and duration of exposures, with maximum severity in 5X and 10X groups on the 30th day. After recommended therapeutic exposure, fillet residues were below the maximum residual limit accepted by the European Union (1000 μg Kg-1) from day three of the withdrawal, showing a minimum three-day is necessary to reach a safe, acceptable level.
{"title":"Assessment of Biosafety and Fillet-Residues After Florfenicol Exposures in Trachinotus Blochii to Ensure Safe-Applications in Disease Incidences","authors":"Krupesha Sharma S.R., Sumithra T.G., R. P, A. K, Ambarish P. Gop, R. R., A. Joshy, V. Prasad, S. G, R. R., Panda S.K., T. G., Anil M.K., P. K. Patil","doi":"10.4194/trjfas22862","DOIUrl":"https://doi.org/10.4194/trjfas22862","url":null,"abstract":"Trachinotus blochii is a promising mariculture fish species. Scientific data on biosafety and fillet residues of florfenicol exposure, one recommended amphenicol antimicrobial for aquaculture use, remains unknown in T. blochii, despite its criticality for prudent application. Accordingly, the paper evaluated the safety (regarding mortality, symptoms, weight gain, and histopathology) of dietary florfenicol after therapeutic (10 mg Kg-1 for ten days) and excessive (three, five, and ten times the therapeutic dose for 10, 20, and 30 days) exposures. There was no mortality in any group. The clinical abnormalities were noted only in 10X group from the 25th exposure day, which disappeared on the fourth day after withdrawal. Reduced growth was recorded at 5X and 10X groups from 20 and 30 exposure days, respectively. Histological lesion`s severity was in the liver > kidney > gill > spleen > muscle > intestine. The lesion severity relied on the quantity and duration of exposures, with maximum severity in 5X and 10X groups on the 30th day. After recommended therapeutic exposure, fillet residues were below the maximum residual limit accepted by the European Union (1000 μg Kg-1) from day three of the withdrawal, showing a minimum three-day is necessary to reach a safe, acceptable level.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48952212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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