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Optical methods for assessing the effect of DON-1R on the histological structure of fish liver 用光学方法评价DON-1R对鱼肝脏组织结构的影响
Pub Date : 2021-12-21 DOI: 10.1117/12.2615589
L. Khuda, O. Khudyi, L. Cheban
With the help of optical methods of fish functional state diagnostics the possibility of using DON-1R, a drug that contains γ–crotonolactone, in recirculating aquaculture system was proven. It was stated that adding DON-1R to granulated feed while feeding sturgeon fish stimulates the raise in mass accumulating velocity. Moreover, microscopical analysis of histological cuts and detailed analysis of their photomicrographs with the use of various approaches in contrasting images showed that feeding with feed with DON-1R is accompanied with reactive changes to the liver. In particular, fish that received the tested drug had the inequable liver cytoarchitectonics, chaotic hepatocytes placement, radial character of liver channels and undefined sinusoids. Hepatocytes of experimental fish had large hyperchromatic nuclei with 4,5 μm diameter with multiple tiny defined nucleoli. Control fish’s nuclei were 1,5 times smaller, had even coloring and 1-2 nucleoli. Moreover, fish from experimental group had signs of partial acidophilic coagulation, such as occurrence of homogenous pink lumps in cytoplasm (Mallory bodies). It is worth noting that the application of DON-1R does not lead to deep destructive changes in liver structure, such as necrosis of fibrosis. Taking all of the above into consideration, DON-1R is the most effective for growth stimulation if applied for a short period of time for commercial growing. Optimal period of use is 20 days.
通过鱼类功能状态的光学诊断方法,证实了含有γ -巴豆内酯的药物DON-1R在循环水养殖系统中应用的可能性。研究表明,在颗粒饲料中添加DON-1R能提高鲟鱼的质量积累速度。此外,对组织学切口的显微分析和使用不同方法对其显微照片的详细分析表明,用含有DON-1R的饲料喂养肝脏伴随着反应性变化。特别是,接受测试药物的鱼的肝脏细胞结构不均匀,肝细胞位置混乱,肝通道呈放射状,正弦曲线不明确。实验鱼的肝细胞有直径为4.5 μm的大深染核,有多个小而清晰的核仁。对照鱼的细胞核小1.5倍,颜色均匀,核仁1-2个。此外,实验组的鱼有部分嗜酸凝血的迹象,如细胞质中出现同质的粉红色肿块(Mallory小体)。值得注意的是,DON-1R的应用不会导致肝脏结构的深度破坏性改变,如纤维化坏死。综上所述,DON-1R在短期内用于商业生长时刺激生长最有效。最佳使用期限为20天。
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引用次数: 0
Diagnostics of haemostasis efficiency with laser illumination scattering pattern analysis 激光照明散射模式分析诊断止血效率
Pub Date : 2021-12-21 DOI: 10.1117/12.2618481
F. Grynchuk, I. Dutka, R. Besaha
Laser illumination scattering by blood samples during the thrombus formation and corresponding changes in scattering patterns for visible spectral range have been studied. Diode laser emission bands with central wavelengths of 0.63, 0.50 and 0.40 μm have been utilized in the single-source and combined illumination configurations. Dynamics of the scattering pattern in terms of its central spot characteristic size has been analyzed and referenced with histological examination. The obtained results demonstrate the applicability of the scattering pattern analysis for assessment of the blood clot formation stage. The prospects for practical diagnostics of the haemostasis efficiency in the emergency medicine with the help of the proposed method have been discussed.
研究了血栓形成过程中血液样品的激光照射散射及其在可见光谱范围内散射模式的变化。中心波长分别为0.63、0.50和0.40 μm的二极管激光发射带可用于单源和组合照明。根据其中心斑点特征尺寸分析了散射模式的动力学,并参考了组织学检查。所得结果证明了散射模式分析在血凝块形成阶段评估中的适用性。讨论了应用该方法对急诊医学止血效率进行实际诊断的前景。
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引用次数: 0
Dynamics of carbon nanoparticles distribution in reconstruction of optical field 光场重建中碳纳米颗粒分布动力学研究
Pub Date : 2021-12-21 DOI: 10.1117/12.2615628
D. Ivanskyi, V. Tkachuk, Jun Zheng, A. I. Dobosh, I. Mikirin
This study proposes a new approach for correlation-optical diagnostics of a complex optical field created by light scattering on a rough surface. The possibility of using fluorescent nanoparticles of a size of about 30-120 nm for diagnosing the distribution of optical flows in the speckle field has been analyzed. A model experiment for investigation motion of carbon nanoparticles in a speckle field is demonstrated. Under the influence of gradient optical forces, carbon nanoparticles move in an optical field and are captured by the singularities of the field. The change of particle position in time in a three-dimensional field due to the action of longitudinal and transverse component of optical force is demonstrated.
本研究提出了一种对粗糙表面光散射产生的复杂光场进行相关光学诊断的新方法。分析了利用30 ~ 120nm大小的荧光纳米粒子诊断散斑场光流分布的可能性。研究了碳纳米粒子在散斑场中运动的模型实验。在梯度光力的作用下,碳纳米粒子在光场中运动,并被光场的奇点捕获。在三维场中,由于光力的纵向和横向分量的作用,粒子的位置随时间的变化。
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引用次数: 0
Correlation-optical method for determining the localization of phase singularities in a scattered field 确定散射场中相位奇点定位的相关光学方法
Pub Date : 2021-12-21 DOI: 10.1117/12.2615563
A. Maksimyak, P. Maksimyak, I. D. Nesteryuk
We proposed an experimental method for determining the localization of phase field singularities. The method consists in measuring the correlation functions of the field and intensity for local fields by several pixels, calculating the ratio of their correlation radii and pixel-by-pixel binding of these parameters to the entire field. Computer simulations and experimental studies have shown that for practically all developed speckle fields, the ratio of the correlation radii saturates in the far detection zone at a value of about 0.64–0.7.
提出了一种确定相场奇点局域化的实验方法。该方法是通过若干像素测量局部场的场和强度的相关函数,计算它们的相关半径的比值以及这些参数与整个场逐像素的绑定。计算机模拟和实验研究表明,对于几乎所有已开发的散斑场,相关半径比在远探测区达到饱和值约为0.64-0.7。
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引用次数: 0
Dynamic interferometry method for measuring wavelength 波长测量的动态干涉法
Pub Date : 2021-12-21 DOI: 10.1117/12.2616223
M. Sakhnovskyj, M. T. Strinadko, M. M. Dominikov, B. Tymochko
It is theoretically proved the possibility of spectral selection of optical signal using the Michelson interferometer, one of the mirrors of which moves, providing the time variation of the path difference of one of beams of the interferometer according to a sawtooth law. It is shown that even a small displacement of the movable mirror (on the order a wavelength), at a predetermined controlled speed, due to the selective amplification of the received total power of the signal allows to detect the spectral density of optical radiation of a specified wavelength and to expand spectral range of the measurements, consistently changing, while maintaining the linearity of the offset mirrors, the speed of its movement. The limit value of the resolving power of the spectrometer is calculated, which in the optical range is 103 - 104 Hz, and is associated with the value of the time constant of the amplifier.
从理论上证明了利用迈克尔逊干涉仪进行光信号光谱选择的可能性,该干涉仪的一个反射镜是移动的,给出了该干涉仪的一个光束的程差按锯齿规律随时间的变化。结果表明,由于接收到的信号总功率的选择性放大,即使移动镜的一个小位移(在一个波长的数量级上),在预定的控制速度下,也可以检测到指定波长的光辐射的光谱密度,并扩大测量的光谱范围,在保持偏移镜的线性的同时,始终改变其运动速度。计算了光谱仪的分辨能力的极限值,该极限值在光学范围内为103 ~ 104 Hz,并与放大器的时间常数值相关联。
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引用次数: 0
Influence of electric field on electronic optical quantum transitions in a quantum dot - quantum ring semiconductor nanostructure 电场对量子点-量子环半导体纳米结构中电子光量子跃迁的影响
Pub Date : 2021-12-21 DOI: 10.1117/12.2615553
I. Hnidko, Vasyl Gutsul, Ivan Koziarskyi, Oleksandr Makhanets
In the model of effective masses and rectangular potentials, the influence of a homogeneous electric field on the energy spectrum, electron wave functions, and oscillator strengths of intraband quantum transitions in a semiconductor (GaAs/AlxGa1-xAs) quantum dot-quantum ring nanostructure is theoretically investigated. In the presence of an electric field, the stationary Schrödinger equations for quasiparticles are not analytically solved. For their approximate solution, the unknown wave functions are sought in the form of an expansion over a complete set of cylindrically symmetric wave functions, and the electron energy is found from the solution of the corresponding secular equation. It is shown that the electric field significantly affects the localization of the electron in the multilayer nanostructure. In this case, both the electron energy and the strength of the oscillators of intraband quantum transitions depend nonmonotonically on the magnitude of the electric field strength.
在有效质量和矩形势模型中,从理论上研究了均匀电场对半导体(GaAs/AlxGa1-xAs)量子点-量子环纳米结构能带内量子跃迁能谱、电子波函数和振子强度的影响。在电场存在下,准粒子的稳态Schrödinger方程不能解析求解。对于未知波函数的近似解,以在一组完整的圆柱对称波函数上展开的形式寻求,并从相应的长期方程的解中找到电子能量。结果表明,电场对多层纳米结构中电子的定位有显著影响。在这种情况下,带内量子跃迁的电子能量和振子的强度都非单调地依赖于电场强度的大小。
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引用次数: 0
Involvement of optical methods for condition assessment of cyanobacteria cells under the action of TiO2 二氧化钛作用下蓝藻细胞状态评估的光学方法研究
Pub Date : 2021-12-21 DOI: 10.1117/12.2615530
L. Cheban, O. Khudyi, L. Vasina, L. Khuda, M. Marchenko
Under optimal conditions, the division of unicellular cyanobacteria lasts 6-12 hours, so during the growing season there is an avalanche of reproduction. It also leads to increased exports of their metabolites to the environment. The toxins of Cyanobacterial after the destruction of these cells enter the aquatic environment and cause poisoning of aquatic organisms and animals and people who consume poisoned water. To avoid the release of toxins, it is necessary to control the rate of accumulation of cyanobacterial biomass. It is proposed to use titanium oxide (IV) as an adsorbent to regulate the number of cyanobacteria. TiO2 has a number of advantages: chemical and biological inertness, non-toxicity, high photostability. The aim of the work was to involve methods of microscopy of native and stained drugs to assess the physiological state of Microcystis pulverea (H.C. Wood) Forti under the action of TiO2. Microcystis pulverea was grown on Fitzgerald medium in the modification of Zender and Gorham №11, at a temperature of 21 ° C and a 16-hour photoperiod in a climatic room. To the cell biomass (4.5 x 106 cells / ml) was made white powder TIO2 in different concentrations: 0.5%, 1%, 2.5%, 5%. To assess the condition of M. pulverea cells after exposure to titanium (IV) oxide, microscopy of objects was performed. Native cells were analyzed for the presence of protective mu-cous formations. The localization of mucous formations on the surface of TiO2 was evaluated. Differential staining was used to determine the presence of living and dead cells and the number of metabolically active cells. These indicators make it possible to assess the condition of cyanobacterial cells under the influence of various environmental factors. To deter-mine the number of dead cells, the cells were stained with vital dyes (methylene blue and neutral red - 1: 5000). To determine the number of physiologically active cells and cytochrome oxidase test were performed according to standard methods. In both cases, cells were counted using a Fuchs-Rosenthal camera and a Micromed XS-3300 trinocular microscope. In samples with high concentrations of titanium dioxide, the cells remained unprotected. It is noted that the mucus was adsorbed around TIO2. The lowest degree of adsorption was characterized by samples containing 0.5% TiO2, and the highest degree - with 5% TiO2. When using TiO2, more than 2.5% can completely release cells of cyanobacteria in the aqueous medium from the mucous that protects their cells, and then use other drugs for lysis. An increase in the number of dead cells was observed in cyanobacterial culture. In M. pulverea culture, the number of metabolically active cells decreases sharply with increasing concentration of TiO2. Incubation with TiO2 led to a gradual decrease in the number of metabolically active cells: from 96% (in the control sample) to about 9% in the presence of 5% TiO2. Although this culture remains relatively alive, but loses the ability to active
在最佳条件下,单细胞蓝藻的分裂持续6-12小时,因此在生长季节会有大量繁殖。这也导致它们的代谢物向环境的出口增加。这些细胞被破坏后,蓝藻的毒素进入水生环境,导致水生生物、动物和饮用有毒水的人中毒。为了避免毒素的释放,有必要控制蓝藻生物量的积累速度。建议使用氧化钛(IV)作为吸附剂来调节蓝藻的数量。TiO2具有许多优点:化学和生物惰性、无毒、高光稳定性。本研究的目的是利用天然药物和染色药物的显微方法来评估TiO2作用下的微囊藻(Microcystis pulverea, H.C. Wood) Forti的生理状态。微囊藻pulverea生长在菲茨杰拉德培养基上的修改Zender和Gorham№11,在21°C的温度和16小时的光周期在气候室。对细胞生物量(4.5 × 106个细胞/ ml)制备不同浓度的TIO2白色粉末:0.5%、1%、2.5%、5%。为了评估M. pulverea细胞暴露于氧化钛(IV)后的状况,对物体进行了显微镜观察。分析原生细胞是否存在保护性的黏液形成。对TiO2表面黏液形成的定位进行了评价。鉴别染色用于确定活细胞和死细胞的存在以及代谢活性细胞的数量。这些指标使得评估各种环境因素影响下蓝藻细胞的状况成为可能。为了确定死亡细胞的数量,用重要染料(亚甲蓝和中性红- 1:50 000)对细胞进行染色。按标准方法测定生理活性细胞数和细胞色素氧化酶测定。在这两种情况下,使用Fuchs-Rosenthal相机和Micromed XS-3300三目显微镜对细胞进行计数。在含有高浓度二氧化钛的样品中,细胞没有受到保护。注意到黏液被吸附在TIO2周围。TiO2含量为0.5%时吸附度最低,TiO2含量为5%时吸附度最高。当使用TiO2时,超过2.5%可以将水介质中蓝藻细胞从保护其细胞的粘液中完全释放出来,然后使用其他药物进行裂解。在蓝藻培养中观察到死细胞数量的增加。在M. pulverea培养中,随着TiO2浓度的增加,代谢活性细胞的数量急剧减少。用TiO2孵育导致代谢活性细胞的数量逐渐减少:从96%(在对照样品中)到约9%(在5% TiO2存在下)。这种培养物虽然保持了相对的活力,但失去了主动代谢的能力。可以预见,随着时间的推移,M. pulverea细胞的数量将急剧减少,这将导致整个培养物的死亡。
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引用次数: 0
Thermal image for monitoring crack forming process 用于裂纹形成过程监测的热图像
Pub Date : 2021-12-21 DOI: 10.1117/12.2615339
Tetiana Venkel, J. Son, Jung Kim, Hyoung Lee, Gwanghee Heo
Thermal image is used to visualize the crack forming and progressing process in mortar prisms added with carbon fibers of five different percentages of 0.0 %, 0.5 %, 1.0 %, 1.5 % and 2.0% in volume, with the time variations of the surface temperature of the prisms while applying forces through a universal testing machine. The surface temperatures are smoothly increasing as the force increases but they reveal sudden temperature increases at the time of crack forming in the prisms. It turns that the sudden temperature increase in the prisms is appeared at the aggregated carbon fibers along the crack paths. The time of increasing matches closely to the crack on-set time determined by a panchromatic camera. Since the fibers along the crack path are more stressed for the given force because they resist more to the force than the mortar itself, their temperatures increase much more than the neighboring mortar. Hence the temperature variation in the aggregated fibers along the crack path informs before the actual crack appearance.
采用热成像技术,在万能试验机上观察了添加0.0%、0.5%、1.0%、1.5%和2.0%体积百分比碳纤维的砂浆棱柱在受力过程中裂纹的形成和发展过程,以及棱柱表面温度的时间变化。表面温度随力的增加而平稳升高,但在棱柱裂纹形成时,表面温度突然升高。结果表明,沿裂纹路径聚集的碳纤维出现了棱柱温度的突然升高。增加的时间与全色相机测定的裂纹开启时间非常接近。由于裂缝路径上的纤维在给定的力下受力更大,因为它们比砂浆本身承受更大的力,因此它们的温度比邻近的砂浆升高得多。因此,在实际裂纹出现之前,聚集纤维沿裂纹路径的温度变化就会被告知。
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引用次数: 0
Optical control of colour deviation due to ink showing through on the banknote reverse on multitoned watermarks 光学控制因油墨透过钞票背面的多色调水印而产生的色彩偏差
Pub Date : 2021-12-21 DOI: 10.1117/12.2615968
T. Kyrychok, N. Talimonova, O. P. Sokol, Ya. Yu. Talimonov
As the inhomogeneity of the paper structure in the areas of watermarks increases, the accuracy of the colour characteristics on the original print changes, as well as the uneven ink showing through on the back of the print, what causes unjustified withdrawal of suitable banknotes due to misjudgement of their condition as worn out. Therefore, to ensure the proper quality of automated sorting of banknotes, a methodology for predicting changes in the colour of the print return on special purpose paper should be developed taking into account its structural characteristics and a methodology for determining quantitative values of paper clearance characteristics with halftone watermarks.
由于水印区域的纸张结构不均匀性增加,原始印刷品颜色特征的准确性发生变化,以及印刷品背面的油墨不均匀,导致由于错误判断其状况而不合理地提取合适的钞票。因此,为了确保自动分拣钞票的适当质量,应考虑到特殊用途纸张的结构特点,开发一种预测其印刷结果颜色变化的方法,并开发一种确定带有半色调水印的纸张间隙特性的定量值的方法。
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引用次数: 0
Gram-Charlier distribution in statistical problems of optics 光学统计问题中的Gram-Charlier分布
Pub Date : 2021-12-21 DOI: 10.1117/12.2615631
V. Zhytaryuk, E. Kurek
In statistical problems of optics to assess the nature of the distribution of random variables and in modern research in the field of signal processing, there is an interest in using Chebyshev-Hermit functions for coding and decoding of signals, since signal components can be described by Gram-Charlier distribution, which has universal properties, can substitute of all known distributions of random variables, and in the case of symmetry takes the form of a Gaussian distribution. A striking feature of skewness and kurtosis is their property of geometric interpretation. It is shown that the application of these parameters in decoding makes it possible to identify the overlapping signals. In statistic problems of rough surface optics, the use of distribution allows to explain some effects, in particular the spectral decomposition of white light observed at grazing angles, and the shift of the maximum specular reflection from the direction of specular luster to the right or left depending on the asymmetry sign.
在光学统计问题中评估随机变量分布的性质以及在信号处理领域的现代研究中,人们对使用切比雪夫-隐士函数对信号进行编码和解码很感兴趣,因为信号成分可以用Gram-Charlier分布来描述,它具有普遍的性质,可以代替所有已知的随机变量分布,并且在对称的情况下采用高斯分布的形式。偏态和峰度的一个显著特征是它们的几何解释性质。结果表明,这些参数在译码中的应用使重叠信号的识别成为可能。在粗糙表面光学的统计问题中,使用分布可以解释一些效应,特别是在掠角处观察到的白光的光谱分解,以及根据不对称符号,最大镜面反射从镜面光泽方向向右或向左移动。
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引用次数: 0
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International Conference on Correlation Optics
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