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First record of three Endophytic fungi isolated from xerophyte plants in Basra, Iraq 首次记录从伊拉克巴士拉的旱生植物中分离出的三种内生真菌
Pub Date : 2024-06-11 DOI: 10.36077/kjas/2024/v16i2.13694
Lina K. Awad, M. Fayyadh
This study was conducted with the aim of isolating endophytic fungi from some xerophyte plants. In this study, three fungi were isolated from the internal tissues of some plants spread in the city of Basrah, Xenomyrothecium tongaense from the stem of Suaeda aegyptiace, Chaetomium megalocarpum from the roots, stem and leaves of Caroxylon jordanicola and Cephaliophora irregularis from the leaves of Cressa cretica. Fungus was identified based on its morphological features and molecular based on the amplification of the ITS gene region and the nucleolide sequence of the fungi was deposited in the genebank under accession number NR-154511.1, KT371335.1 and OM245865.1 for fungi Xenomyrothecium tongaense, Chaetomium megalocarpum and Cephaliophora irregularis, respectively. This is the first study in which these fungi are isolated from desert in Iraq.
这项研究旨在从一些旱生植物中分离内生真菌。在这项研究中,从分布在巴士拉市的一些植物的内部组织中分离出了三种真菌:从 Suaeda aegyptiace 的茎中分离出了 Xenomyrothecium tongaense;从 Caroxylon jordanicola 的根、茎和叶中分离出了 Chaetomium megalocarpum;从 Cressa cretica 的叶中分离出了 Cephaliophora irregularis。根据形态特征对真菌进行了鉴定,并根据 ITS 基因区的扩增对真菌进行了分子鉴定,真菌的核苷酸序列保存在基因库中,Xenomyrothecium tongaense、Chaetomium megalocarpum 和 Cephaliophora irregularis 的登录号分别为 NR-154511.1、KT371335.1 和 OM245865.1。这是首次从伊拉克沙漠中分离出这些真菌的研究。
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引用次数: 0
Processing browning and increasing multiplication and rotting of Peach rootstock cv. Garnem by using antioxidants and plant growth regulators in vitro 利用抗氧化剂和植物生长调节剂体外处理桃砧木 Garnem 的褐变,提高其繁殖率和腐烂率使用抗氧化剂和植物生长调节剂进行体外培植
Pub Date : 2024-06-11 DOI: 10.36077/kjas/2024/v16i2.10942
Sarah Ali Muhammad Al-Hayany, A. A. Obaid
The study aimed to find an accurate propagation program for the rootstock of peach Prunus persica cv. Garnem. The apical shoots and nodes of this rootstock were used as explants for the stages of initiation and multiplication, and they were planted on MS medium after adding different concentrations of Active Charcoal (AC), Polyvinyl Pyrrolidone (PVP), and Benzyl adenine (BA). The shoots were planted for the purpose of rooting on MS medium containing different concentrations of salts (1/4 MS, 1/2 MS and full MS salts) with different concentrations of IBA. In the establishment phase, the apical shoots grown on medium supplied with AC were given the highest response rate, the highest average length of plantlet, and the highest number of leaves. The best multiplication of explants in terms of the number of shoots and their length were obtained when cultivated on a medium supplemented with BA at 1 and 2 mg.L-1, whereas the application of BA at 1 mg. L-1 to the cultivated nodes gave the highest number of leaves. The best rooting percent was obtained when plantlets were s treated with 2 mg. L-1 was applied to 1/4 MS medium. The highest number of roots was obtained from plantlets grown in 1/2 MS medium treated with IBA at 3 mg.L-1, whereas the longest root was obtained from plantlets grown in 1/2 MS medium treated with 1 mg.L-1IBA.
该研究旨在为桃 Prunus persica cv. Garnem 的砧木找到准确的繁殖方案。该砧木的顶端嫩枝和节被用作萌发和繁殖阶段的外植体,它们被种植在添加了不同浓度的活性炭(AC)、聚乙烯吡咯烷酮(PVP)和苄基腺嘌呤(BA)的 MS 培养基上。在含有不同浓度盐分(1/4 MS 盐分、1/2 MS 盐分和全 MS 盐分)和不同浓度 IBA 的 MS 培养基上种植嫩枝,目的是使其生根。在生根阶段,在含有 AC 的培养基上生长的顶芽反应率最高、小植株平均长度最高、叶片数最多。在添加了 1 和 2 mg.L-1 BA 的培养基上培养的外植体在芽的数量和长度方面的繁殖率最高,而在 1 mg.L-1 的培养基上添加 BA 的外植体在芽的数量和长度方面的繁殖率最低,而在 1 mg.L-1 的培养基上添加 IBA 的外植体在芽的数量和长度方面的繁殖率最低。而在培养节上施用 1 mg.L-1 的 BA,叶片数量最多。在 1/4 MS 培养基中添加 2 mg.L-1 BA 处理的小植株生根率最高。L-1 的培养基时,生根率最高。在用 3 mg.L-1 的 IBA 处理过的 1/2 MS 培养基中生长的植株生根数最多,而在用 1 mg.L-1IBA 处理过的 1/2 MS 培养基中生长的植株生根最长。
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引用次数: 0
Effect of Aspergillus flavus filtrate on different stages of Callosobruchus maculatus (Fabricius.) (Chrysomelidae: Coleoptera) on Broad beans 黄曲霉菌滤液对蚕豆上不同阶段的大茧蜂(Callosobruchus maculatus (Fabricius.) (Chrysomelidae: Coleoptera)的影响
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.3632
Karrar Mohammed Hussein Al-Hamadani, F. A. Sabit
An experiment was conducted to use A.flavus filtrate in treating different stages of C.maculatus (eggs, larvae, pupae) after diluting it with sterile distilled water at concentrations 30%, 50%, 70% directly to calculate the killing rates after (1, 3, 5) days After treatment, the best results of egg inhibition were in the fifth day, with rates reaching 36.9, 53.7 and 83.87%  respectively, and the best results of killing larvae were in the 3rd, 5th, and 5th day, respectively, with rates reaching 50.33, 80.47and 93.93% respectively, and the best results were Killing of the pupa in the 5th, 5th, and 5th day respectively, with rates that reached 63.50, 86.93and 96.97% respectively, and the best results of killing for adults were in the 3rd, 5th, and5th days respectively, with rates that reached 43.73, 90.83, and 93.93% respectively. The best results of killing were in the 1th, 3th, and 5th day respectively on adults of C. maculatus via the indirect method, as the highest killing rates were at the concentration of 70% and reached 56.83, 60.36 and 67.1% respectively, The best results were on the amount of laying eggs from an adult C. maculatus and by direct and indirect spraying on females. The results showed that the lowest rate of laying eggs for one female after three days of mating was zero eggs/female with a concentration of 70% compared to the indirect method which reached to 24.13 eggs/female.
本试验采用黄曲霉滤液分别用浓度为30%、50%、70%的无菌蒸馏水稀释后直接处理不同阶段的黄斑弧菌(卵、幼虫、蛹),计算处理后(1、3、5)d的杀虫率。处理后第5 d的抑卵效果最佳,抑卵率分别达到36.9%、53.7和83.87%,第3、5、5 d的杀虫效果最佳。杀蛹率分别为50.33、80.47和93.93%,第5、5、5天杀蛹效果最好,杀蛹率分别为63.50、86.93和96.97%,第3、5、5天杀成虫效果最好,杀蛹率分别为43.73、90.83和93.93%。间接法对黄斑夜蛾成虫杀灭效果最佳的时间分别为第1天、第3天和第5天,杀灭率在70%浓度时最高,分别为56.83%、60.36%和67.1%,以产卵量和直接法和间接法对雌虫杀灭效果最佳。结果表明,交配3 d后,1只雌蜂的产卵率最低,为0个/只,浓度为70%,而间接产卵法的产卵率为24.13个/只。
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引用次数: 0
Evaluation of the effectiveness of the boiled extract, regular and nano oil of some medicinal plants and their effect on the eggs and larvae of the third phase of date moth Ephestia Cautella (Walk.) (Lepidoptera:Pyralidae) in laboratory conditions 在实验室条件下评估一些药用植物的煮沸提取物、普通油和纳米油的功效及其对枣树蛾 Ephestia Cautella (Walk.) (Lepidoptera:Pyralidae) 第三阶段卵和幼虫的影响
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.3662
Afrah Denar Hadi, M. M. Dewan, T. M. Taha
The experiment was conducted under laboratory conditions, in the insect lab at the College of Science for Girls / University of Kufa in 2021.In order to test the effect of extracts, regular and nano-oil of cinnamon, Cinnamomum zeylanicu, turmeric Curcuma longa and Elettaria cardamomum on the eggs and larvae of the third phase of date moth. Where the three nano-oils excelled on mortality eggs, where the mortality rate of eggs was 100% compared to the regular oil and the boiled extract. The nano-oils were also missed in mortality rate of the third phase larvae after 72 treatments. The Nano cinnamon oil  was excelled in mortality larvae, where the mortality rate was 94.4%, compared to “turmeric nano oil and nano cardamom oil, respectively, as it reached 89.2 and 61.8% at 2% concentration. Keywords: plant extracts, essential oils, nano oils, Ephestia Cautella.
该实验于2021年在库法大学女子科学学院的昆虫实验室在实验室条件下进行。为了测试肉桂、肉桂、姜黄、小豆蔻提取物、常规油和纳米油对三期枣蛾卵和幼虫的影响。三种纳米油在死亡鸡蛋上表现出色,与普通油和煮过的提取物相比,鸡蛋的死亡率是100%。72次处理后,第三期幼虫的死亡率也未发现纳米油。纳米肉桂油对幼虫的死亡率为94.4%,高于“姜黄纳米油”和“纳米豆蔻油”,浓度为2%时,其死亡率分别为89.2和61.8%。关键词:植物提取物;精油;纳米精油;
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引用次数: 0
Effect of supplemental light, benzyl adenine (BA) on the growth and flowering of two cultivar of Chrysanthemum plant (Dendranthema grandiflora Tzvelev) 补充光照、苄基腺嘌呤(BA)对两种菊花(Dendranthema grandiflora Tzvelev)生长和开花的影响
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.10325
Y. Hammo, Jwan I. Saleem
  This study was conducted during the period from 23rd September 2021 to 1st May 2022 in Horticulture stations / Akre / Iraqi Kurdistan region, amid to scheduled the flower production of Chrysanthemum (Dendrathema grandiflora Tzvelev), by use the supplemental light with (Natural day, 1.5-month, 3-month) treatment, and BA with 0, 200 and 400 mg.l-1 and two cultivars Avalon (pink) and Flirt (red). The best results include, use 3-month of supplemental light significantly increased the day to flower bud, days to anthesis to 143.36, 201.10 day respectively compared with 1.5 month which need 119.61 , 175.02 day and control which need 49.28, 78.31day for the two characteristics respectively this mean that 3-month delay the flower production by 122.79 day than control, Plant high, the flower grade and Plant growth index also increased significantly with increased the supplemental light to 1.5 and 3-month compared with control. spray Chrysanthemum with BA increased significantly the day to flower bud emergence, vase life and flower grade compared with control. the Flirt (red) cultivar significantly superior than Avalon (pink) in day flower bud emergence, day flowering anthesis, plant high and flower grade.Key Words: supplemental light, benzyl adenine, cultivars, chrysanthemum
本研究于2021年9月23日至2022年5月1日在伊拉克库尔德斯坦地区的阿克雷园艺站进行,在预定的菊花(Dendrathema grandiflora Tzvelev)开花期间,采用(自然日、1.5个月、3个月)补光处理,BA浓度分别为0、200和400 mg。l-1和两个品种Avalon(粉红色)和Flirt(红色)。最好的结果是,使用3个月的补光处理显著提高了开花、开花所需的时间,分别为143.36、201.10天,而1.5个月的补光处理分别为119.61、175.02天和对照分别为49.28、78.31天,这意味着3个月的补光处理比对照延迟了122.79天的开花时间。补光量增加到1.5个月和3个月,花的等级和植株的生长指数也显著高于对照。与对照相比,喷施BA显著提高了菊花的出芽日、瓶期和花的品级。在日花蕾出芽、日花期、株高、花品位等方面,红色的Flirt品种均显著优于粉红色的Avalon品种。关键词:补光,苄基腺嘌呤,品种,菊花
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引用次数: 0
The possibility of manufacturing a biocidal of Bacillus spp. and their growth on different fermented media 制造芽孢杆菌杀菌剂的可能性及其在不同发酵培养基上的生长情况
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.10356
Ali Salman Abbas, Wissam Adnan R. Aljuaifari
The study was conducted in the Molecular Biology Laboratory - Department of Plant Protection - Faculty of Agriculture - University of Kufa during 2020-2021 to evaluate the possibility of producing a biocide from Bacillus spp. B. firmus, B. cereus. and B. simplex. The results of manufacturing bionematicide from these bacteria showed that the three species did not differ in their growth on the same compost medium (palm fronds, rice residues, wheat residues), while the growth of each Bacillus spp. differed from other species on different culture media. The palm frond medium, regardless of the type of bacteria, was the least favorable and did not support many bacterial populations, and allowed no more than 83*108 CFU. Whereas the bacterial colonies ranged from 219*108 CFU to 254*108 CFU on fermented wheat residues, with a slight difference from rice residues, which led to bacterial colonies of 198*108 CFU to 214*108 CFU. In the case of carriers used for the biocide, preparation using sand was always better than with calcium sulfate. The effect of packing materials (plastic bags, paper bags, or plastic containers) on the efficiency and vitality of bacteria in the biocide content was also evaluated. It is noted that the highest bacterial colonies, which ranged from 267 to 253 *108 CFU, in the case of using plastic containers significantly differed from storing in paper bags (233-230*108 CFU) and plastic bags (235 -216 *108 CFU), after 15 days of storage in laboratory conditions. However, the viability and effectiveness of the prepared biocide decreased with the increase of storage periods to 30, 60, and 90 days, regardless of the type of bacteria.
该研究于2020-2021年在库法大学植物保护系农学院分子生物学实验室进行,以评估从芽孢杆菌、硬芽孢杆菌和蜡样芽孢杆菌中生产杀菌剂的可能性。B.单纯性;结果表明,3种芽孢杆菌在相同的堆肥培养基(棕榈叶、稻渣、小麦渣)上的生长无显著差异,但各芽孢杆菌在不同培养基上的生长存在差异。无论细菌类型如何,棕榈叶培养基都是最不利的,支持的细菌群体不多,允许的细菌数量不超过83*108 CFU。小麦发酵渣菌落在219*108 ~ 254*108 CFU之间,与水稻发酵渣菌落在198*108 ~ 214*108 CFU之间差异不大。在杀菌剂载体的情况下,用沙子制备总是比用硫酸钙制备好。还评估了包装材料(塑料袋、纸袋或塑料容器)对杀菌剂含量中细菌的效率和活力的影响。在实验室条件下储存15天后,使用塑料容器的细菌菌落最高,为267 ~ 253 *108 CFU,与纸袋(233 ~ 230*108 CFU)和塑料袋(235 ~ 216 *108 CFU)有显著差异。然而,无论细菌类型如何,制备的杀菌剂的活力和有效性随着储存时间的增加而下降,分别为30、60和90天。
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引用次数: 0
Detection of post-harvest insecticide Flash 10% EC residues in wheat seeds and soil after its degradation by the UV-C 检测收获后杀虫剂 Flash 10% EC 在紫外线-C 降解后在小麦种子和土壤中的残留量
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.3679
Adeeb K Al-Shafiee, Jamal H. Kadhim
This study aimed to test the effect of the physical factor (UV-C) in degrading of insecticide Flash residue in wheat seeds and the soil post-harvest. As for the field experiments with regard to Flash residual in the soil, the results showed that the highest concentration of the insecticide was 3.10 ppm in the seeds  produced from plants treated with Flash only, while the lowest concentration (below the detectable level) was in the wheat seeds from plants sprayed with UV-C treated Flash. As for the residual in the soil, the highest active compound concentration was 12.50 ppm appeared in the uncultivated soil treated with Flash untreated, compared to the lowest residual concentration (0.00) ppm was in soil planted with wheat plants treated with UV-C treated Flash. Findings also showed that the best exposure period to the UV-C was 20mn  
本研究旨在考察物理因子(UV-C)对小麦种子和收获后土壤中农药闪蒸残留的降解效果。在田间试验中,对土壤中闪蒸菌残留量的测定结果表明,只施用闪蒸菌的种子中闪蒸菌残留量最高,为3.10 ppm,而施用UV-C闪蒸菌的小麦种子中闪蒸菌残留量最低(低于检测水平)。在土壤残留方面,未施用闪蒸的未耕土壤中活性化合物浓度最高,为12.50 ppm,而施用UV-C闪蒸的小麦植株土壤中活性化合物残留量最低,为0.00 ppm。研究结果还表明,UV-C的最佳暴露时间为2000万年
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引用次数: 0
Volatile blooms of Aleurodicus rugioperculatus Martin infested coconut and banana leaves attracting parasitoid Encarsia guadeloupae Viggiani Aleurodicus rugioperculatus Martin 在椰子和香蕉叶上的挥发性绽放吸引寄生虫 Encarsia guadeloupae Viggiani
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.12142
Gunalan Chandrasekaran, Jeyarani Subramanian, M. Marimuthu
Invasive whitefly species in coconut plantations have become a matter of concern in the last half a decade as they cause direct and indirect infestation on the palms. The Rugose spiralling whitefly (RSW), Aleurodicus rugioperculatus Martin infestation was high on Cocos nucifera L. (coconut) followed by Dypsis lutescens (H. Wendl.) (Butterfly palm) and Annona squamosa L. (custard apple). The coconut varieties Malayan Yellow Dwarf and Chowghat Orange Dwarf were observed with higher infestation index while that of West coast tall was the lowest. Higher RSW parasitization levels were observed on Musa paradisiaca L. (banana) and Canna indica L. (Indian shot), with 85.96 and 71.59% parasitization, respectively. Identification of volatile organic compounds (VOCs) between healthy and RSW-infested coconut plants revealed the emission of 56 VOCs from the healthy coconut plant and 47 VOCs from RSW infested plant.  22 VOCs were common in both samples, and 25 VOCs were unique to RSW-infested coconut plants. The presence of 42 VOCs was identified from the headspace extracts of RSW-infested bananas. Differences in the VOCs emitted from RSW-infested banana and coconut plants revealed higher emission of terpenoids like β-Caryophyllene, (E, Z)-2,6-dimethyl-2,4,6-Octatriene, Humulene, α-Pinene, Farnesane, α-Copaene and β-cis-Ocimene from RSW infested banana plants that proved to be more attractive to the parasitoid. Identifying specific blends of volatile compounds influencing Encarsia guadeloupae Viggiani could help to augment the parasitotic for RSW management in coconut plantations.
在过去的五年里,椰子种植园中的白蝇入侵物种已经成为一个令人担忧的问题,因为它们对棕榈树造成了直接和间接的侵扰。Cocos nucifera L.(椰子)、Dypsis lutescens (H. Wendl.)(蝴蝶棕榈)和Annona squamosa L.(蛋奶苹果)侵染最多的是Rugose螺旋体白蝇(RSW)、Aleurodicus rugioperculatus Martin。马来亚黄矮和Chowghat橙矮的侵染指数较高,西海岸高的侵染指数最低。香蕉和美美蕉的RSW寄生率分别为85.96%和71.59%。对健康椰树和被RSW侵染的椰树的挥发性有机化合物(VOCs)进行了鉴定,发现健康椰树的挥发性有机化合物(VOCs)排放量为56种,被RSW侵染的椰树的挥发性有机化合物(VOCs)排放量为47种。22种挥发性有机化合物在两个样本中都是常见的,25种挥发性有机化合物是被rsw感染的椰子植物所特有的。从被rsw感染的香蕉顶空提取物中鉴定出42种挥发性有机化合物。被RSW侵染的香蕉和椰子植株挥发性有机化合物的差异表明,被RSW侵染的香蕉植株释放的β-石竹烯、(E, Z)-2,6-二甲基-2,4,6-辛三烯、Humulene、α-蒎烯、法尔内烷、α-Copaene和β-顺式辛三烯等萜类化合物更高,对寄生蜂更有吸引力。鉴定影响瓜德罗普沙蚕的挥发性化合物的特定混合物有助于增加椰子种植园RSW的寄生管理。
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引用次数: 0
The effect of some food sources and hive products on the oriental hornet Vespa orientalis Linnaeus 1771 (Hymenoptera: Vespidae) 一些食物来源和蜂巢产品对东方大黄蜂 Vespa orientalis Linnaeus 1771(膜翅目:蚜蝇科)的影响
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.10391
Afrah Abdulzahraa Aljassani, M. T. Al-Esawy, R. S. Augul
This study was carried out in the bee laboratory in the Faculty of Agriculture –University of Kufa in September 2021 to evaluate some protein sources and hive products against the oriental hornet Vespa orientalis Linnaeus 1771 (Hymenoptera: Vespidae). The food sources included: beef meat, gut fish, beef lung, honeybee, wax, propolis, pollen, bee worker, and water, in addition to control which was an empty petri dish. The number of visits and their duration were calculated. The results showed that the wasps preferred bee honey and wax significantly higher than the rest of the stimuli.
本研究于2021年9月在库法大学农学院蜜蜂实验室进行,目的是评估一些蛋白质来源和蜂箱产品对东方大黄蜂(膜翅目:大黄蜂科)1771的抗性。食物来源包括:牛肉,鱼肠,牛肺,蜜蜂,蜂蜡,蜂胶,花粉,蜂工和水,另外还有一个空的培养皿作为对照。计算了访问次数和持续时间。结果表明,蜂对蜂蜜和蜂蜡的偏好明显高于其他刺激。
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引用次数: 0
Effect of fertilization of Seaweed extracts and CuSO4 on some vegetative growth indicators of Citrus limon L. grafted seedlings on rootstock aurantifolia 海藻提取物和 CuSO4 施肥对砧木枳壳上嫁接幼苗一些无性生长指标的影响
Pub Date : 2023-12-01 DOI: 10.36077/kjas/2023/v15i2.10426
Abrar Moneam Almoussawi, G. B. Al-Abbasi
Citrus aurantifolia rootstock seedlings were treated with Seaweed extracts and copper sulfate. Seaweed extracts were added as a ground feed during the growing season at four levels (0, 10, 20, and 30 ml. seedling-1) and copper sulfate sprayed at four concentrations (15,10,5, 0 ml. L-1) alone or in combination with the control (watering with water only) during the growing seasons 2020-2021. The results showed that the treatment of seedlings grafted on rootstock aurantifolia with Seaweed extracts and spraying with copper sulfate affected (height, stem diameter, area Leaf, root length, and chlorophyll content of leaves (the results also showed that the addition of Seaweed extracts at a concentration of 30 ml overlapping with copper sulfate spraying at a concentration of 15 ml. L-1 is recommended to improve the vegetative, root and nutritional growth of lemon citrus seedlings.
以紫菜提取物和硫酸铜处理金叶柑桔砧木幼苗为研究对象。在2020-2021年的生长季节,以4种浓度(0、10、20和30 ml.幼苗-1)添加海藻提取物作为地面饲料,并以4种浓度(15、10、5、0 ml. L-1)单独喷洒硫酸铜或与对照(仅用水浇水)联合喷洒。结果表明,用紫菜提取物和硫酸铜喷施对嫁接在桔梗砧木上的幼苗的株高、茎粗、叶面积、根长和叶片叶绿素含量均有影响(结果还表明,添加浓度为30 ml的紫菜提取物与浓度为15 ml L-1的硫酸铜重叠喷施可改善柠檬柑橘幼苗的营养、根系和营养生长。
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引用次数: 0
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Kufa Journal for Agricultural Sciences
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