: Chronic wound resulting from diabetes mellitus is a significant cause of amputation world-wide. Secondary infections, lowering of nitric oxide synthase level, reduction of glucose-6-phosphate dehydrogenase levels, improper extracellular matrix remodelling, neuropathy, abnormality of endothelial cell function, and vasculopathy impedes the normal wound healing cycle during diabetes. Multiple studies have concluded that Ser9 phosphorylation causes inhibition of the glycogen synthase kinase-3 β (GSK3-β ) protein, which is essential for faster diabetic wound healing. Hence this protein could be a potential target for molecular interactions with prospective wound-healing molecules. Verbascoside, martynoside, echinacoside, crenatoside, and salidroside are a few phenylethanoid glycosides that have potential wound-healing ability by increasing extracellular matrix synthesis, angiogenesis, keratinocyte migration, and the functioning of macrophages and neutrophils. Thus, the five glycosides were subjected to molecular docking with GSK3-β protein (PDB ID: 1I09). This study revealed strong binding interactions with GSK3-β (between − 10.2 and − 7.3 kcal/mol) and inhibition constants (between 0.032 and 4.397 µ M) which suggested potent inhibition of the target protein even at lower concentrations of these compounds. Further, the docked complexes were visualized to find the interaction of the ligands with the amino acid residues. However, further in vivo and in vitro studies are required to validate the activity of these phenylpropanoid glycosides in diabetic wound healing.
{"title":"In Silico Study to Evaluate the Inhibitory Activity of a Few Phenylethanoid Glycosides on GSK3-β Protein for Faster Diabetic Wound Healing","authors":"Ankit Majie, Rajdeep Saha, B. Sarkar","doi":"10.3390/ecb2023-14134","DOIUrl":"https://doi.org/10.3390/ecb2023-14134","url":null,"abstract":": Chronic wound resulting from diabetes mellitus is a significant cause of amputation world-wide. Secondary infections, lowering of nitric oxide synthase level, reduction of glucose-6-phosphate dehydrogenase levels, improper extracellular matrix remodelling, neuropathy, abnormality of endothelial cell function, and vasculopathy impedes the normal wound healing cycle during diabetes. Multiple studies have concluded that Ser9 phosphorylation causes inhibition of the glycogen synthase kinase-3 β (GSK3-β ) protein, which is essential for faster diabetic wound healing. Hence this protein could be a potential target for molecular interactions with prospective wound-healing molecules. Verbascoside, martynoside, echinacoside, crenatoside, and salidroside are a few phenylethanoid glycosides that have potential wound-healing ability by increasing extracellular matrix synthesis, angiogenesis, keratinocyte migration, and the functioning of macrophages and neutrophils. Thus, the five glycosides were subjected to molecular docking with GSK3-β protein (PDB ID: 1I09). This study revealed strong binding interactions with GSK3-β (between − 10.2 and − 7.3 kcal/mol) and inhibition constants (between 0.032 and 4.397 µ M) which suggested potent inhibition of the target protein even at lower concentrations of these compounds. Further, the docked complexes were visualized to find the interaction of the ligands with the amino acid residues. However, further in vivo and in vitro studies are required to validate the activity of these phenylpropanoid glycosides in diabetic wound healing.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114221435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lorenzo Notari, P. Nardini, V. Grandi, Alessandro Corsi, N. Pimpinelli, S. Bacci
{"title":"Neuroimmunomodulation in Chronic Wound Healing after Treatment with Photodynamic Therapy: The Role of iNOs","authors":"Lorenzo Notari, P. Nardini, V. Grandi, Alessandro Corsi, N. Pimpinelli, S. Bacci","doi":"10.3390/ecb2023-14135","DOIUrl":"https://doi.org/10.3390/ecb2023-14135","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129803662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Diabetes mellitus (DM) is a chronic metabolic disorder and is associated with impaired wound healing. Non-healing leg and foot ulcers are a frequent significant consequence of diabetes and are caused by a combination of inadequate tissue perfusion, suppression of re-epithelialization, and poor collagen production. Receptor for Advanced Glycation End Products (RAGE) is a multi-ligand cell surface molecule that belongs to the immunoglobulin superfamily and is crucial in the pathophysiology of poor wound healing in diabetics. By inhibiting RAGE, a chronic non-healing wound is more likely to undergo angiogenesis, enhance blood supply to hypoxic areas of the wound, and decrease the pro-inflammatory reaction and pro-apoptotic signaling. Phenylethanoid glycosides (PhGs) are a class of natural glycosides that possess anti-diabetic, wound-healing, antimicrobial, anti-inflammatory, and antioxidant properties. Echinacoside, a phenylethanoid glycoside, has a promising role in wound healing by enhancing angiogenesis, promoting keratinocyte migration and proliferation, and enhancing neutrophil and macrophage activity. Consequently, molecular docking was performed to assess the interaction between Echinacoside and the RAGE receptor (PDB ID: 6VXG). The ligand and receptor had a strong binding interaction, as indicated by the lowest binding energy, which was found to be − 6.1 kcal/mol. To further assess the activity of Echinacoside in diabetic wound healing, in vitro and in vivo studies are needed.
糖尿病(DM)是一种慢性代谢紊乱，与伤口愈合受损有关。无法愈合的腿脚溃疡是糖尿病常见的严重后果，是由组织灌注不足、再上皮化抑制和胶原蛋白生成不良共同引起的。晚期糖基化终产物受体(Receptor for Advanced Glycation End Products, RAGE)是一种多配体细胞表面分子，属于免疫球蛋白超家族，在糖尿病患者伤口愈合不良的病理生理中起着至关重要的作用。通过抑制RAGE，慢性不愈合创面更容易发生血管生成，增加创面缺氧区域的血供，减少促炎反应和促凋亡信号。苯乙醇糖苷(PhGs)是一类具有抗糖尿病、伤口愈合、抗菌、抗炎和抗氧化特性的天然糖苷。紫锥菊苷是一种苯乙醇苷，通过促进血管生成，促进角质细胞迁移和增殖，增强中性粒细胞和巨噬细胞活性，在伤口愈合中具有良好的作用。因此，我们进行了分子对接，以评估紫锥菊苷与RAGE受体(PDB ID: 6VXG)的相互作用。配体与受体具有较强的结合作用，最低结合能为−6.1 kcal/mol。为了进一步评估紫锥花苷在糖尿病创面愈合中的作用，还需要进行体内和体外研究。
{"title":"An In Silico Approach to Evaluate the Diabetic Wound Healing Potential of Phenylethanoid Glycoside in Inhibiting the Receptor for Advanced Glycation End Products (RAGE)","authors":"Ritika Baidya, B. Sarkar","doi":"10.3390/ecb2023-14137","DOIUrl":"https://doi.org/10.3390/ecb2023-14137","url":null,"abstract":": Diabetes mellitus (DM) is a chronic metabolic disorder and is associated with impaired wound healing. Non-healing leg and foot ulcers are a frequent significant consequence of diabetes and are caused by a combination of inadequate tissue perfusion, suppression of re-epithelialization, and poor collagen production. Receptor for Advanced Glycation End Products (RAGE) is a multi-ligand cell surface molecule that belongs to the immunoglobulin superfamily and is crucial in the pathophysiology of poor wound healing in diabetics. By inhibiting RAGE, a chronic non-healing wound is more likely to undergo angiogenesis, enhance blood supply to hypoxic areas of the wound, and decrease the pro-inflammatory reaction and pro-apoptotic signaling. Phenylethanoid glycosides (PhGs) are a class of natural glycosides that possess anti-diabetic, wound-healing, antimicrobial, anti-inflammatory, and antioxidant properties. Echinacoside, a phenylethanoid glycoside, has a promising role in wound healing by enhancing angiogenesis, promoting keratinocyte migration and proliferation, and enhancing neutrophil and macrophage activity. Consequently, molecular docking was performed to assess the interaction between Echinacoside and the RAGE receptor (PDB ID: 6VXG). The ligand and receptor had a strong binding interaction, as indicated by the lowest binding energy, which was found to be − 6.1 kcal/mol. To further assess the activity of Echinacoside in diabetic wound healing, in vitro and in vivo studies are needed.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132102355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Breast cancer (BC) is one of the leading causes of death in Canadian women, with an average survival rate of 5 years after diagnosis. Early detection of BC can greatly improve patient outcomes and survival. However, a non-invasive BC detection method is not currently available in clinics. Recent studies suggest that proteins from small extracellular vesicles (sEVs) could be promising biomarkers for non-invasive BC early-stage diagnosis. sEVs are membrane-enclosed vesicles secreted by cells that drive different stages of carcinogenesis in BC. The purpose of this work was to analyze different published proteomics datasets to identify enzymes that could be potentially used as diagnostic biomarkers. Three cell line studies were compared, and overlapping BC proteins were highlighted with proteins found in sEVs from blood and plasma. In total, 106 proteins were selected based on the cell line studies, of which 40 have been identified in blood/plasma sEVs. These 106 proteins were mostly enriched with cell–cell signaling and DNA repair terms based on GO analysis. Furthermore, these 40 proteins contained 11 enzymes that can be explored as potential BC biomarkers. Future validation of enzymes using cancer cell lines and blood from BC patients remains to be determined.
{"title":"Proteomics Approaches for the Discovery of Potential Enzymatic Biomarkers for Early Diagnosis of Breast Cancer","authors":"Yingxi Li, N. Hüttmann, Z. Minić, M. Berezovski","doi":"10.3390/ecb2023-14099","DOIUrl":"https://doi.org/10.3390/ecb2023-14099","url":null,"abstract":"Breast cancer (BC) is one of the leading causes of death in Canadian women, with an average survival rate of 5 years after diagnosis. Early detection of BC can greatly improve patient outcomes and survival. However, a non-invasive BC detection method is not currently available in clinics. Recent studies suggest that proteins from small extracellular vesicles (sEVs) could be promising biomarkers for non-invasive BC early-stage diagnosis. sEVs are membrane-enclosed vesicles secreted by cells that drive different stages of carcinogenesis in BC. The purpose of this work was to analyze different published proteomics datasets to identify enzymes that could be potentially used as diagnostic biomarkers. Three cell line studies were compared, and overlapping BC proteins were highlighted with proteins found in sEVs from blood and plasma. In total, 106 proteins were selected based on the cell line studies, of which 40 have been identified in blood/plasma sEVs. These 106 proteins were mostly enriched with cell–cell signaling and DNA repair terms based on GO analysis. Furthermore, these 40 proteins contained 11 enzymes that can be explored as potential BC biomarkers. Future validation of enzymes using cancer cell lines and blood from BC patients remains to be determined.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115502833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. L. Hernández-Bule, Elena Toledano-Macías, María Antonia Martínez-Pascual, A. Úbeda, M. Fernández-Guarino
: Wound healing consists of a sequence of coordinated phases: inflammation, proliferation, and remodeling. In skin lesions, neutrophils and keratinocytes are the main cell types participating in the inflammatory phase, during which the release of mediators intervening in the regulation of the subsequent regenerative phases takes place. These mediators are involved in tissue regeneration through induction of transendothelial migration, enzyme secretion, cell adhesion, and T-Cell activation and cytotoxicity, as well as neutrophil accumulation at the wound site. Among these mediators, the keratinocyte-synthesized chemokines RANTES, MCP-1, MIP-1, and IL-8 stand out. Although therapies applying electromagnetic fields or electric currents have been shown to have anti-inflammatory effects in a variety of experimental models and in patients through reduced production of proinflammatory cytokines such as IFN-Υ and increased production of IL-10, the knowledge on the biological basis of these effects is still limited. Previous studies by our group have shown that subthermal treatment with radiofrequency (RF) currents used in capacitive-resistive electric transfer (CRET) therapy promotes the proliferation and migration of various cell types, such as human ADSC (stem cells), fibroblasts or keratinocytes, involved in skin regeneration. This study investigates the effects of in vitro treatment with CRET currents on cytokine production by HaCat human keratinocytes. The results reveal that, compared to sham-exposed controls, RF stimulation induces decreased production of IL-8 and RANTES and increased MCP-1, without significantly affecting other chemokines such as MIP-1. Taken together, our results indicate that due to the RF effects on the production of chemokines involved in the modulation of the inflammatory phase of wound regeneration, CRET therapy could be effective in the treatment of skin wounds.
{"title":"Effects of RF Currents on Cytokines Production in Human Keratinocytes","authors":"M. L. Hernández-Bule, Elena Toledano-Macías, María Antonia Martínez-Pascual, A. Úbeda, M. Fernández-Guarino","doi":"10.3390/ecb2023-14096","DOIUrl":"https://doi.org/10.3390/ecb2023-14096","url":null,"abstract":": Wound healing consists of a sequence of coordinated phases: inflammation, proliferation, and remodeling. In skin lesions, neutrophils and keratinocytes are the main cell types participating in the inflammatory phase, during which the release of mediators intervening in the regulation of the subsequent regenerative phases takes place. These mediators are involved in tissue regeneration through induction of transendothelial migration, enzyme secretion, cell adhesion, and T-Cell activation and cytotoxicity, as well as neutrophil accumulation at the wound site. Among these mediators, the keratinocyte-synthesized chemokines RANTES, MCP-1, MIP-1, and IL-8 stand out. Although therapies applying electromagnetic fields or electric currents have been shown to have anti-inflammatory effects in a variety of experimental models and in patients through reduced production of proinflammatory cytokines such as IFN-Υ and increased production of IL-10, the knowledge on the biological basis of these effects is still limited. Previous studies by our group have shown that subthermal treatment with radiofrequency (RF) currents used in capacitive-resistive electric transfer (CRET) therapy promotes the proliferation and migration of various cell types, such as human ADSC (stem cells), fibroblasts or keratinocytes, involved in skin regeneration. This study investigates the effects of in vitro treatment with CRET currents on cytokine production by HaCat human keratinocytes. The results reveal that, compared to sham-exposed controls, RF stimulation induces decreased production of IL-8 and RANTES and increased MCP-1, without significantly affecting other chemokines such as MIP-1. Taken together, our results indicate that due to the RF effects on the production of chemokines involved in the modulation of the inflammatory phase of wound regeneration, CRET therapy could be effective in the treatment of skin wounds.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125625720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Pronina, S. Lukina, V. Loginov, A. Burdennyy, T. Kazubskaya, E. Braga, E. Filippova
Breast cancer (BC) remains one of the leading causes of cancer deaths among women worldwide. Recently, studies of long non-coding RNAs (lncRNAs) involved in the regulation of various signaling pathways in cells have become increasingly important, since they demonstrate great prognostic potential in cancer. The aim of our work was to identify new aberrantly expressed lncRNAs in BC. We identified 30 aberrantly expressed lncRNAs in BC. For most lncRNAs, a decrease in the expression level by 2.34–13.2 times (p < 0.05) was noted, and only for lncRNA TERC, an increase in the expression level by 2.24 times (p = 0.034) was noted. Of greatest interest are the data obtained for the lncRNAs ADAMTS9-AS2, EMX2OS, HOTAIRM1 and MEG3, as they are consistent with the data of the bioinformatic analysis.
{"title":"Experimental Identification of Aberrantly Expressed Long Non-Coding RNAs in Breast Cancer","authors":"I. Pronina, S. Lukina, V. Loginov, A. Burdennyy, T. Kazubskaya, E. Braga, E. Filippova","doi":"10.3390/ecb2023-14083","DOIUrl":"https://doi.org/10.3390/ecb2023-14083","url":null,"abstract":"Breast cancer (BC) remains one of the leading causes of cancer deaths among women worldwide. Recently, studies of long non-coding RNAs (lncRNAs) involved in the regulation of various signaling pathways in cells have become increasingly important, since they demonstrate great prognostic potential in cancer. The aim of our work was to identify new aberrantly expressed lncRNAs in BC. We identified 30 aberrantly expressed lncRNAs in BC. For most lncRNAs, a decrease in the expression level by 2.34–13.2 times (p < 0.05) was noted, and only for lncRNA TERC, an increase in the expression level by 2.24 times (p = 0.034) was noted. Of greatest interest are the data obtained for the lncRNAs ADAMTS9-AS2, EMX2OS, HOTAIRM1 and MEG3, as they are consistent with the data of the bioinformatic analysis.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121620030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Petrova, Nikol Mazhdrakova, S. Todinova, V. Strijkova, M. Zhiponova, S. Krumova
.net/. Abstract: We show that an extract from catmint ( Nepeta nuda L.) flowers is not hemotoxic and does not alter erythrocytes’ morphology. H 2 O 2 -induced oxidative stress leads to an increase in lipid peroxidation, accompanied by a reduction in the number of biconcave cells and an increase in the number of echinocytes. Pre-treatment of erythrocytes with this extract does not reduce the lipid peroxidation level; however, it results in partial restoration of the relative abundance of biconcave cells and a respective reduction in the echinocytes’ quantity. Our data reveal the concentrations at which the examined extract exhibits a protective effect on erythrocytes’ morphology under the condition of H 2 O 2 -induced oxidative stress.
{"title":"Nepeta nuda L. Plant Extract Preserves the Morphology of Red Blood Cells Subjected to Oxidative Stress","authors":"S. Petrova, Nikol Mazhdrakova, S. Todinova, V. Strijkova, M. Zhiponova, S. Krumova","doi":"10.3390/ecb2023-14086","DOIUrl":"https://doi.org/10.3390/ecb2023-14086","url":null,"abstract":".net/. Abstract: We show that an extract from catmint ( Nepeta nuda L.) flowers is not hemotoxic and does not alter erythrocytes’ morphology. H 2 O 2 -induced oxidative stress leads to an increase in lipid peroxidation, accompanied by a reduction in the number of biconcave cells and an increase in the number of echinocytes. Pre-treatment of erythrocytes with this extract does not reduce the lipid peroxidation level; however, it results in partial restoration of the relative abundance of biconcave cells and a respective reduction in the echinocytes’ quantity. Our data reveal the concentrations at which the examined extract exhibits a protective effect on erythrocytes’ morphology under the condition of H 2 O 2 -induced oxidative stress.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131264742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Dominant optic atrophy (DOA), mainly caused by pathogenic variants in OPA1 , is one of the most common forms of hereditary optic neuropathy. OPA1 is involved in mitochondrial dynamics and oxidative phosphorylation, among other functions. Hence, mutations in this gene cause the degeneration of retinal ganglion cells (RGCs), leading to reduced visual acuity. In this work, we have used induced pluripotent stem cell (iPSC) technology to generate RGCs, starting from an iPSC line created from fibroblasts from a DOA patient and also its CRISPR isogenic control. The generated RGCs showed expression of BRN3A, SNCG or THY1, and could potentially serve as a platform for DOA modeling.
{"title":"Generation of iPSC-Derived RGCs for Modeling Dominant Optic Atrophy","authors":"Marta García-López, M. E. Gallardo","doi":"10.3390/ecb2023-14087","DOIUrl":"https://doi.org/10.3390/ecb2023-14087","url":null,"abstract":": Dominant optic atrophy (DOA), mainly caused by pathogenic variants in OPA1 , is one of the most common forms of hereditary optic neuropathy. OPA1 is involved in mitochondrial dynamics and oxidative phosphorylation, among other functions. Hence, mutations in this gene cause the degeneration of retinal ganglion cells (RGCs), leading to reduced visual acuity. In this work, we have used induced pluripotent stem cell (iPSC) technology to generate RGCs, starting from an iPSC line created from fibroblasts from a DOA patient and also its CRISPR isogenic control. The generated RGCs showed expression of BRN3A, SNCG or THY1, and could potentially serve as a platform for DOA modeling.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131872679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: The drugs currently available as cyclooxygenase-2 (COX-2) inhibitors increase the risk of cardiovascular events, which justifies the search for new alternative anti-inflammatory molecules. This work aimed to evaluate the anti-inflammatory activity of the chalcones (polyhydroxylated aromatic compounds): 2 (cid:48) ,3,4,4 (cid:48) ,6 (cid:48) -pentahydroxychalcone (5OH), 2 (cid:48) ,3,4,4 (cid:48) ,6 (cid:48) -pentamethoxychalcone (5OMe), and 2 (cid:48) ,3,4,4 (cid:48) -tetrahydroxychalcone (butein), at non-cytotoxic concentrations, in an experimental human whole blood model. The results obtained showed that none of the chalcones under study was cytotoxic to human blood cells. From the tested chalcones, butein was the only one showing a concentration-dependent inhibitory production of prostaglandin E 2 , via COX-2, in human blood (40 ± 8%, at 50 µ M).
{"title":"Research into New Molecules with Anti-Inflammatory Activity","authors":"Abel Vale, M. Lucas, D. Ribeiro, E. Fernandes","doi":"10.3390/ecb2023-14095","DOIUrl":"https://doi.org/10.3390/ecb2023-14095","url":null,"abstract":": The drugs currently available as cyclooxygenase-2 (COX-2) inhibitors increase the risk of cardiovascular events, which justifies the search for new alternative anti-inflammatory molecules. This work aimed to evaluate the anti-inflammatory activity of the chalcones (polyhydroxylated aromatic compounds): 2 (cid:48) ,3,4,4 (cid:48) ,6 (cid:48) -pentahydroxychalcone (5OH), 2 (cid:48) ,3,4,4 (cid:48) ,6 (cid:48) -pentamethoxychalcone (5OMe), and 2 (cid:48) ,3,4,4 (cid:48) -tetrahydroxychalcone (butein), at non-cytotoxic concentrations, in an experimental human whole blood model. The results obtained showed that none of the chalcones under study was cytotoxic to human blood cells. From the tested chalcones, butein was the only one showing a concentration-dependent inhibitory production of prostaglandin E 2 , via COX-2, in human blood (40 ± 8%, at 50 µ M).","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115433183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Signature Garlic Phytochemical as a Potential Anti-Candidal Candidate Targeting Virulence Factors in Candida albicans ","authors":"Z. Hasan, A. Islam, L. Khan","doi":"10.3390/ecb2023-14080","DOIUrl":"https://doi.org/10.3390/ecb2023-14080","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133209330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：