Anuja D. Kamble, A. Kumbhar, Rashmi P. Kulkarni, Anjali A. Kulkarni
{"title":"Potential Hypoglycemic Secondary Metabolites from Argyreia nervosa (Burm. f.) Bojer Influencing Human Gut Health","authors":"Anuja D. Kamble, A. Kumbhar, Rashmi P. Kulkarni, Anjali A. Kulkarni","doi":"10.3390/ecb2023-14090","DOIUrl":"https://doi.org/10.3390/ecb2023-14090","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129554577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: The problem of pharmacologically correcting CNS hypoxic disorders is one of priority. HSP70, an endogenous regulator of cytoprotective processes, can be considered as an effective pharmacological target. The aim of this research was to study the ability of cerebrocurin, angiolin, glutoredoxin, tamoxifen, thiotriazoline, L-arginine, nikomex, HSF-1 and piracetam to modulate the level of HSP70 in the cerebral cortex and blood plasma of rats after prenatal hypoxia (PH). We studied the effect of the drugs on the content of HSP70 in the plasma and neurons (cytoplasmic and mitochondrial fractions) of rat pups on the 30th and 60th day of life in model of chronic hemic PH using the enzyme immunoassay method. It was found that PH leads to the suppression of HSP70 synthesis and to the decrease in its intra-and extracellular levels, with the most significant decrease during the 1st month of life. Drugs course administration demonstrates an increase in intracellular and extracellular levels of HSP70 with a prolonged effect. Cerebrocurin, angiolin, and tamoxifen were the most active modulators of intracellular HSP70. Cerebrocurin, angiolin, and piracetam had the most active effect on the HSP70 content in blood plasma, but the effect of piracitam on the cytosolic and mitochondrial HSP70 fractions was the least of all the drugs studied. Here, we show that cerebrocurin and angiolin were the most effective modulators of HSP70, and their neuroprotective effect deserves further comprehensive study in order to develop methods for effective treatment of hypoxic disorders. HSP70 can serve as a target and marker of hypoxia pharmacological correction.
{"title":"Modulation of Hsp70 in the Pharmacological Correction of Nervous System Disorders after Prenatal Hypoxia","authors":"O. Aliyeva, I. Belenichev, O. Popazova","doi":"10.3390/ecb2023-14091","DOIUrl":"https://doi.org/10.3390/ecb2023-14091","url":null,"abstract":": The problem of pharmacologically correcting CNS hypoxic disorders is one of priority. HSP70, an endogenous regulator of cytoprotective processes, can be considered as an effective pharmacological target. The aim of this research was to study the ability of cerebrocurin, angiolin, glutoredoxin, tamoxifen, thiotriazoline, L-arginine, nikomex, HSF-1 and piracetam to modulate the level of HSP70 in the cerebral cortex and blood plasma of rats after prenatal hypoxia (PH). We studied the effect of the drugs on the content of HSP70 in the plasma and neurons (cytoplasmic and mitochondrial fractions) of rat pups on the 30th and 60th day of life in model of chronic hemic PH using the enzyme immunoassay method. It was found that PH leads to the suppression of HSP70 synthesis and to the decrease in its intra-and extracellular levels, with the most significant decrease during the 1st month of life. Drugs course administration demonstrates an increase in intracellular and extracellular levels of HSP70 with a prolonged effect. Cerebrocurin, angiolin, and tamoxifen were the most active modulators of intracellular HSP70. Cerebrocurin, angiolin, and piracetam had the most active effect on the HSP70 content in blood plasma, but the effect of piracitam on the cytosolic and mitochondrial HSP70 fractions was the least of all the drugs studied. Here, we show that cerebrocurin and angiolin were the most effective modulators of HSP70, and their neuroprotective effect deserves further comprehensive study in order to develop methods for effective treatment of hypoxic disorders. HSP70 can serve as a target and marker of hypoxia pharmacological correction.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127549548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Development of Medical Shampoo with a Plant-Based Substance for the Treatment of Seborrheic Dermatitis","authors":"V. Filatov, O. Kulyak, E. Kalenikova","doi":"10.3390/ecb2023-14084","DOIUrl":"https://doi.org/10.3390/ecb2023-14084","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123765768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martina Donozo, V. Cavallaro, C. J. Baier, A. Murray
: Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder. Acetylcholinesterase (AChE) inhibitors constitute a pharmacotherapeutic strategy for AD treatment. Resveratrol has anti-inflammatory, neuroprotective
{"title":"New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line","authors":"Martina Donozo, V. Cavallaro, C. J. Baier, A. Murray","doi":"10.3390/ecb2023-14094","DOIUrl":"https://doi.org/10.3390/ecb2023-14094","url":null,"abstract":": Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder. Acetylcholinesterase (AChE) inhibitors constitute a pharmacotherapeutic strategy for AD treatment. Resveratrol has anti-inflammatory, neuroprotective","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132439503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HPLC Screening of Phytoestrogens from Soybeans in Conjunction with Chemometric Data Analysis: A Tool for Selecting the Best Raw Materials for Producing Dietary Supplements for Menopausal Health","authors":"E. Muntean, C. Urdǎ, R. Rezi","doi":"10.3390/ecb2023-14082","DOIUrl":"https://doi.org/10.3390/ecb2023-14082","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131954560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pokkittath Radhakrishnan Arya, P. Abishad, Varsha Unni, Mohan Bibin, Dias Marita, Lijo John, A. Karthikeyan, P. Nambiar, S. Juliet, V. K. Vinod, J. Vergis, N. Kurkure, S. Barbuddhe, D. Rawool
Enteroaggregative Escherichia coli (EAEC) has been widely recognized as one of the leading causes of infantile diarrhoea and nutrient malabsorption in developing as well as developed countries. In recent years, drug resistance, particularly multi-drug resistance (MDR), among the EAEC strains has been widely documented and could result in a therapeutic stumble. Antimicrobial alternatives are widely employed to curb this emerging public health crisis. In the present study, a facile one-pot synthesis of silver/zinc oxide nanocomposites (Ag/ZnO NCs) using a methanolic extract of stem and leaves of Curcuma longa was performed. The synthesis of Ag/ZnO NCs was confirmed using UV-vis spectroscopy and Fourier transform infrared spectroscopy, while the thermal stability was ascertained by thermogravimetric analysis with differential thermogravimetric analyses, and crystallinity was determined using powder X-ray diffraction. The shape and size of the green synthesized Ag/ZnO NCs, determined using field-emission–scanning-electron microscopy and transmission electron microscopy, revealed an irregular polycrystalline morphology with a size of 31.34 ± 1.27 nm. Later, the antibacterial potential of the green synthesized Ag/ZnO NCs evaluated against MDREAEC strains revealed a minimum inhibitory concentration of 31.25 μg/mL and a minimum bactericidal concentration ranging from 62.50 to 125 μg/mL. Moreover, the green synthesized Ag/ZnO NCs inhibited the biofilm-forming ability of the tested strains of MDR-EAEC. Furthermore, concentrationdependent antioxidant activity was exhibited by the green synthesized Ag/ZnO NCs, as evidenced by the ABTS assay and reducing power assay. Overall, this study demonstrated the antibacterial potential of Ag/ZnO NCs synthesized using C. longa extracts with antifouling as well as antioxidant properties, which could be used as an alternative therapeutic candidate.
{"title":"Antioxidant, Antibacterial and Antibiofilm Potential of Green Synthesized Silver-Zinc Oxide Nanocomposites from Curcuma longa Extract against Multi-Drug-Resistant Enteroaggregative E. coli","authors":"Pokkittath Radhakrishnan Arya, P. Abishad, Varsha Unni, Mohan Bibin, Dias Marita, Lijo John, A. Karthikeyan, P. Nambiar, S. Juliet, V. K. Vinod, J. Vergis, N. Kurkure, S. Barbuddhe, D. Rawool","doi":"10.3390/ecb2023-14088","DOIUrl":"https://doi.org/10.3390/ecb2023-14088","url":null,"abstract":"Enteroaggregative Escherichia coli (EAEC) has been widely recognized as one of the leading causes of infantile diarrhoea and nutrient malabsorption in developing as well as developed countries. In recent years, drug resistance, particularly multi-drug resistance (MDR), among the EAEC strains has been widely documented and could result in a therapeutic stumble. Antimicrobial alternatives are widely employed to curb this emerging public health crisis. In the present study, a facile one-pot synthesis of silver/zinc oxide nanocomposites (Ag/ZnO NCs) using a methanolic extract of stem and leaves of Curcuma longa was performed. The synthesis of Ag/ZnO NCs was confirmed using UV-vis spectroscopy and Fourier transform infrared spectroscopy, while the thermal stability was ascertained by thermogravimetric analysis with differential thermogravimetric analyses, and crystallinity was determined using powder X-ray diffraction. The shape and size of the green synthesized Ag/ZnO NCs, determined using field-emission–scanning-electron microscopy and transmission electron microscopy, revealed an irregular polycrystalline morphology with a size of 31.34 ± 1.27 nm. Later, the antibacterial potential of the green synthesized Ag/ZnO NCs evaluated against MDREAEC strains revealed a minimum inhibitory concentration of 31.25 μg/mL and a minimum bactericidal concentration ranging from 62.50 to 125 μg/mL. Moreover, the green synthesized Ag/ZnO NCs inhibited the biofilm-forming ability of the tested strains of MDR-EAEC. Furthermore, concentrationdependent antioxidant activity was exhibited by the green synthesized Ag/ZnO NCs, as evidenced by the ABTS assay and reducing power assay. Overall, this study demonstrated the antibacterial potential of Ag/ZnO NCs synthesized using C. longa extracts with antifouling as well as antioxidant properties, which could be used as an alternative therapeutic candidate.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121513291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agnieszka Mlicka, Katarzyna Siemiątkowska, Iris Plaku, E. Żekanowska, Artur Słomka
: The health-promoting properties of Silybum marianum have been acknowledged since antiquity. This plant is credited with substantial hepatoprotective properties and is also protective in cardiovascular diseases, diabetes mellitus, and neurodegeneration, mainly for its anti-inflammatory and antioxidant effects. Only a few experimental studies have described the impact of Silybum marianum extract on the blood coagulation process; furthermore, these data are unsatisfactorily fragmented and need to be supplemented to understand the plant’s properties better. The predominant biologically active flavonolignan extracted from Silybum marianum is silybin, a mixture of two diastere-omers, silybin A and silybin B, in approximately equimolar ratio. This study investigated the effect of silybin on the fundamental laboratory parameter for blood coagulation, namely prothrombin time (PT), an assay used to assess the extrinsic and common coagulation pathways. To evaluate the effect of silybin on PT, we prepared three solutions of silybin (Silybin (A + B mixture), PhytoLab GmbH & Co. KG, Vestenbergsgreuth, Germany) in 0.1% dimethylsulfoxide (DMSO, Sigma-Aldrich, Co., St. Louis, MO, USA): 10 µ M, 50 µ M, and 100 µ M. PT was measured on a Coag 4D coagulometer (DIAGON Kft., Budapest, Hungary) using rabbit calcium thromboplastin (Dia-PT, DIAGON Kft., Budapest, Hungary) and control plasma, which is pooled plasma obtained from healthy donors (Dia-CONT, DIAGON Kft., Budapest, Hungary). A total of 10 µ L of silybin solution was added to 40 µ L of plasma; the sample was incubated for two minutes at 37 ◦ C, and then 100 µ L of thromboplastin, pre-warmed to 37 ◦ C, and was added to the mixture. The coagulometer automatically gives the PT result in seconds (s). At the same time, PT was measured in the control plasma both without additional solutions and with the addition of tris-buffered saline (TBS) and 0.1% DMSO (10 µ L of TBS or DMSO + 40 µ L of plasma). Each measurement was
{"title":"Silybin, the Main Active Component of Silybum marianum, Affects Blood Coagulation: An In Vitro Pilot Study","authors":"Agnieszka Mlicka, Katarzyna Siemiątkowska, Iris Plaku, E. Żekanowska, Artur Słomka","doi":"10.3390/ecb2023-14081","DOIUrl":"https://doi.org/10.3390/ecb2023-14081","url":null,"abstract":": The health-promoting properties of Silybum marianum have been acknowledged since antiquity. This plant is credited with substantial hepatoprotective properties and is also protective in cardiovascular diseases, diabetes mellitus, and neurodegeneration, mainly for its anti-inflammatory and antioxidant effects. Only a few experimental studies have described the impact of Silybum marianum extract on the blood coagulation process; furthermore, these data are unsatisfactorily fragmented and need to be supplemented to understand the plant’s properties better. The predominant biologically active flavonolignan extracted from Silybum marianum is silybin, a mixture of two diastere-omers, silybin A and silybin B, in approximately equimolar ratio. This study investigated the effect of silybin on the fundamental laboratory parameter for blood coagulation, namely prothrombin time (PT), an assay used to assess the extrinsic and common coagulation pathways. To evaluate the effect of silybin on PT, we prepared three solutions of silybin (Silybin (A + B mixture), PhytoLab GmbH & Co. KG, Vestenbergsgreuth, Germany) in 0.1% dimethylsulfoxide (DMSO, Sigma-Aldrich, Co., St. Louis, MO, USA): 10 µ M, 50 µ M, and 100 µ M. PT was measured on a Coag 4D coagulometer (DIAGON Kft., Budapest, Hungary) using rabbit calcium thromboplastin (Dia-PT, DIAGON Kft., Budapest, Hungary) and control plasma, which is pooled plasma obtained from healthy donors (Dia-CONT, DIAGON Kft., Budapest, Hungary). A total of 10 µ L of silybin solution was added to 40 µ L of plasma; the sample was incubated for two minutes at 37 ◦ C, and then 100 µ L of thromboplastin, pre-warmed to 37 ◦ C, and was added to the mixture. The coagulometer automatically gives the PT result in seconds (s). At the same time, PT was measured in the control plasma both without additional solutions and with the addition of tris-buffered saline (TBS) and 0.1% DMSO (10 µ L of TBS or DMSO + 40 µ L of plasma). Each measurement was","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132897131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: This study aims to analyze the patent documents concerning the uses of medicinally active plants (MAPs) in relation to COVID-19 during the last four years. Hereinafter, we demonstrate that MAPs, such as those in the Lamiaceae family, can be a fast, healthy, and efficient recourse for designing new drugs or compositions to face new health challenges such as the COVID-19 pandemic. The patent documents concerning sage ( Salvia ), basil ( Ocimum ), lavender ( Lavandula )
{"title":"Patent Analysis of Four Lamiaceae-Derived Plants: A Medicinally Active Resource against New Health Challenges †","authors":"Reda El Boukhari, A. Fatimi","doi":"10.3390/ecb2023-14093","DOIUrl":"https://doi.org/10.3390/ecb2023-14093","url":null,"abstract":": This study aims to analyze the patent documents concerning the uses of medicinally active plants (MAPs) in relation to COVID-19 during the last four years. Hereinafter, we demonstrate that MAPs, such as those in the Lamiaceae family, can be a fast, healthy, and efficient recourse for designing new drugs or compositions to face new health challenges such as the COVID-19 pandemic. The patent documents concerning sage ( Salvia ), basil ( Ocimum ), lavender ( Lavandula )","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115794803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shrivastava, D. Chaudhary, L. Shrestha, M. Awadalla, S. Al-Shouli, Anjan Palikhey, W. A. Eltayb, Anamika Gupta, Pramodkumar P. Gupta, M. Parab, Anchal Trivedi, Aditi Srivastava, Mohnad Abdalla
{"title":"GC-MS Based Metabolite Profiling, and Anti-Inflammatory Activity of Aqueous Extract of Myrica esculenta through In Vitro and In Silico Approach","authors":"A. Shrivastava, D. Chaudhary, L. Shrestha, M. Awadalla, S. Al-Shouli, Anjan Palikhey, W. A. Eltayb, Anamika Gupta, Pramodkumar P. Gupta, M. Parab, Anchal Trivedi, Aditi Srivastava, Mohnad Abdalla","doi":"10.3390/ecb2023-14079","DOIUrl":"https://doi.org/10.3390/ecb2023-14079","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128557373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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