ACS Applied Bio Materials最新文献

Breast reconstruction following oncoplastic breast-conserving surgery (OBCS) presents significant challenges, particularly in cases where conventional volume displacement techniques are insufficient. While autologous flaps offer an alternative, they are associated with donor site morbidity and surgical complexity. Acellular dermal matrix (ADM) has emerged as a promising volume replacement option, but its application in direct defect filling remains largely unexplored. This study introduced SC Fill paste, an injectable, microparticulate ADM developed through supercritical carbon dioxide (CO₂) decellularization, micronization, and dispersion, ensuring easy application and adaptability to irregular defect geometries. In vitro and in vivo assessments demonstrated efficient host tissue integration, preservation of the extracellular matrix (ECM) and essential growth factors, and minimal inflammatory response due to low double-stranded DNA (dsDNA) content and the absence of major histocompatibility complex (MHC-I) proteins, as confirmed by Western blot analysis. Additionally, SC Fill paste exhibited enhanced fibroblast infiltration and neovascularization and reduced capsular contracture compared to commercial controls. Matrix metalloproteinase (MMP) activity and collagen expression indicated a consistent six-month remodeling effect, ensuring long-term stability. These findings provide strong preclinical evidence supporting SC Fill paste as a versatile and effective reconstructive filler, offering a practical and adaptable solution for post-BCS defects.

Herein, we harnessed the transformative power of waste sugarcane-derived in situ nitrogen-doped carbon dots (N-CDs) to develop a fluorescent probe, poised to revolutionize the sensitive and selective detection of Bilirubin (BIL) in human urine. By utilizing waste sugarcane bagasse as the source of carbon and nitrogen, we embraced a combination of greener, solvent-free, and chemical-free thermal carbonization and the Sonochemical process to create N-CDs. The electronic, crystallographic, photochemical, and morphological behaviors of the synthesized N-CDs have been meticulously analyzed using various spectroscopic and microscopic techniques. When excited at 360 nm, these N-CDs emit a captivating blue fluorescence at 440 nm with an impressive color purity of 75.1%. Remarkably, the addition of BIL, without the need for labels on the N-CDs, results in a striking selective enhancement of this fluorescence with a striking color purity of 81%. This enhancement is driven by the formation of a ground-state interaction between BIL and the N-CDs, which is confirmed through a particle size analyzer and atomic force microscopy. The turn-on mechanism, which could be due to the formation of pi-pi stacking, is validated through FT-IR, and ¹H-NMR reveals the powerful interactions at play. This exceptional probe exemplifies unparalleled sensitivity, ultrastability, rapid response times, and unmatched accuracy for BIL concentrations ranging from 6.6 to 1876 nM, culminating in a limit of detection (LOD) of 3 nM, showcasing its efficacy in both synthetic urine samples and real human urine sample analysis with impressive recovery results. This study ignites inspiring insights into trailblazing approaches for quantifying BIL levels in urine, paving the way for significant advancements in noninvasive, stable, and accessible alternative medical diagnostics.

Estrogen receptor-positive (ER+) breast cancer is the predominant subtype of breast cancer, having significant therapeutic drawbacks, largely driven by undesired toxicity and drug resistance associated with conventional treatment regimens. Conjugating organoselenium moieties to various drugs or enzyme inhibitors can be a promising approach for the treatment of breast cancer with multimodal benefits in overcoming the limitations of conventional therapies. Herein, we report the synthesis and evaluation of a series of organoselenium–NBDHEX hybrids as potent anticancer agents. Based on the selective antiproliferative activities of these derivatives against ER+ breast cancer cells (MCF-7) over the nonmalignant cells (L132), the hybrid NHSe-2, having a 2-selenocyanatoacetyl linker, was chosen as the lead analogue for further studies. The compound NHSe-2 exhibited notable antiproliferative activity with S-phase arrest of cells and late-phase apoptosis in MCF-7 cells. Most importantly, NHSe-2 induced ROS-mediated degradation of HDAC4, NF-κB, and c-Myc, resulting in potent antiproliferation and eventually leading to apoptosis of MCF-7 cells. Moreover, it suppressed β-catenin expression, unlike NBDHEX; however, it retained GSTP1 inhibitory potency, indicating its add-on therapeutic potential. Therefore, NHSe-2 could be a potential anticancer agent and can be considered for further analysis for its multimodal activity in the realm of cancer research in the future.

Proper wound dressing is essential for ensuring and promoting effective wound healing. This study aimed to develop a novel porous composite wound dressing composed of aloe polysaccharide and collagen. The physical properties of aloe polysaccharide-collagen wound dressing (AP-CD) formulated in this study was characterized by scanning electron microscopy, energy dispersive spectroscopy, water absorption and air permeability tests. The safety and cytocompatibility of AP-CD were evaluated using CCK-8 and lactate dehydrogenase leakage rate assays. Besides, the effectiveness of AP-CD was assessed by measuring the wound healing rate. In vivo studies confirmed that AP-CD improved the wound healing rate and accelerated the healing process. Furthermore, AP-CD suppressed inflammatory response, promoted angiogenesis, increased the levels of growth factors and anti-inflammatory factors, and decreased the levels of pro-inflammatory factors. Single-cell sequencing analysis revealed a significant increase in the proportion of macrophages and T lymphocytes in AP-CD-treated wound tissue, accompanied by a notable decrease in fibroblast proportions. In summary, AP-CD demonstrated superior physical and chemical properties, good biocompatibility, and safety, making it a viable option for wound dressing. In vivo studies indicated that AP-CD effectively inhibited inflammatory responses and enhanced angiogenesis, thereby accelerating wound healing.

Due to the lack of relevant detection methods, the existence and function of the intestinal and placental alkaline phosphatase (IAP-PLAP) heterodimer remain largely elusive. Previously, we screened and obtained an aptamer, BG2, which exhibits specific recognition toward the IAP-PLAP heterodimer. Using BG2 as a probe, the heterodimer was found to be highly expressed on the membrane of various tumor cells as well as circulating tumor cells derived from clinical colorectal cancer samples, thus being regarded as a potential tumor marker. However, whether it is shed into extracellular fluid remains unclear. Herein, we developed a BG2-based chemiluminescence assay method with high sensitivity and selectivity for the detection of the IAP-PLAP heterodimer in biological fluids. Furthermore, based on the Na⁺-dependent binding between BG2 and the IAP-PLAP heterodimer, the captured proteins were successfully released and confirmed indeed IAP-PLAP heterodimer, indicating that they can be shed from the cell membrane into the culture medium. It was also found that the concentration of the IAP-PLAP heterodimer in the cell culture medium is closely correlated with its expression level on the cell membrane. Additionally, the levels of the heterodimer both on the cell membrane and in the culture medium were reduced in senescent cells. These results suggest that the IAP-PLAP heterodimer in body fluids may also serve as a disease marker. We further verified that this method can detect the IAP-PLAP heterodimer spiked in plasma samples with good recoveries, thus providing a method for liquid biopsy.

For activity-based sensing, where probes detect biological analytes through chemical reactivity, nucleic acid (NA) scaffolds represent an attractive platform to enhance biocompatibility and permit ratiometric analyte detection through the precise control of distance and orientation of donor/acceptor probes within the duplex framework for a turn-on FRET response. Herein, we present the first all-in-one NA FRET sensor (KR28) that is activated by H₂O₂ as a molecular trigger in human serum and in the nucleus of live cells. The 28-mer 2′-OMe-XNA hairpin (HP) KR28 features a boronic acid nucleobase surrogate (BA6HI) that serves as the H₂O₂ sensing element and is embedded into KR28 using an on-strand Aldol condensation approach. ipso-Hydroxylation of the BA6HI surrogate mediated by H₂O₂ furnishes an emissive phenolic product (PhOH6HI, λ_ex/λ_em = 390/490 nm) that serves as the FRET donor to a thiophene surrogate acceptor (Th6HI, λ_ex/λ_em = 530/580 nm) for a FRET efficiency ∼ 95%. The BA6HI-modified NA scaffolds can detect H₂O₂ in the low nM regime and localize in the nucleus of live cells for nuclear H₂O₂ FRET detection. Our work expands the function of NA scaffolds beyond their use as molecular recognition elements through noncovalent affinity interactions and demonstrates their potential to serve as activity-based sensors of ROS to probe the relationships between nuclear oxidative stress and disease states.

Extracellular vesicle (EV)-mediated transfer of biomolecules plays an essential role in intercellular communication and presents promising avenues for targeted drug delivery. Over the past decade, researchers have developed various approaches to modifying EV surfaces for targeting specific cells or tissues, including functionalization with targeting peptides to increase the specificity of drug delivery. Due to technical limitations, methods for characterizing the targeting moieties on the surface of small EVs (sEVs) are considerably restricted. To address these limitations and enhance the throughput capacity of sEV characterization, a dual-reporter platform was utilized to quantitatively assess the binding of tumor homing peptide (THP)-functionalized sEVs to breast cancer cells using bioluminescence assays and fluorescence microscopy. Twenty-four scrambled variants of the uPAR-binding peptide were designed for sEV engineering, and their uptake by MDA-MB-231 cells was evaluated in vitro. Our results revealed that amino acid scrambling generated both enhanced and reduced binding to cancer cells compared to the original peptide sequence. Furthermore, the data demonstrated that mechanical stimulation of EV producer HEK293FT cells enhanced the passive loading of methotrexate (MTX) into sEVs, but not large EVs, by increasing sEV production. By functionalizing MTX-loaded sEVs with a high-binding scrambled peptide, the delivery successfully surpassed the saturated free MTX uptake level in MDA-MB-231 cells, increasing cytotoxicity by 2.1-fold and providing a potent strategy for combating drug-resistant cancers. This study advances synthetic biology approaches to optimize tumor-targeted drug delivery, demonstrating that strategic peptide sequence scrambling can enhance targeting efficiency and drug delivery capabilities.

The rising prevalence of multidrug-resistant (MDR) bacterial infections highlights the urgent need for innovative antimicrobial therapies that are both effective and industrially scalable. We report a metal-free, facilely prepared, and biocompatible nanoplatform (TCPP-HF@Lec) designed for synergistic antibacterial treatment via light-triggered codelivery of singlet oxygen (¹O₂) and carbon monoxide (CO). This system coencapsulates the photosensitizer tetrakis(4-carboxyphenyl)porphyrin (TCPP) and the CO-releasing prodrug 3-hydroxyflavone (3-HF) within a lecithin-based nanoparticle, enabling straightforward preparation with potential for large-scale pharmaceutical production. Upon 660 nm irradiation, TCPP generates ¹O₂, which not only induces photodynamic cytotoxicity but also activates 3-HF via oxidative decarbonylation, achieving spatiotemporally controlled CO release. Comprehensive physicochemical characterization revealed uniform morphology, excellent colloidal stability, and efficient dual-agent loading. In vitro, TCPP-HF@Lec exhibited potent, concentration-dependent antibacterial activity against Gram-positive strains, including Staphylococcus aureus TISTR1466, Bacillus subtilis TISTR008, and methicillin-resistant Staphylococcus aureus (MRSA), achieving near-complete eradication under light irradiation. In contrast, Gram-negative bacteria (Escherichia coli TISTR780 and Pseudomonas aeruginosa TISTR781) showed negligible susceptibility, consistent with fluorescence imaging that revealed preferential nanoparticle uptake by Gram-positive bacteria lacking an outer membrane. By combining precise light-controlled activation with a production-friendly design, this dual-modality nanoplatform overcomes major limitations of conventional photodynamic therapy and CO-releasing molecules. TCPP-HF@Lec offers a promising approach for the scalable development of next-generation targeting antibacterial nanotherapeutics against MDR bacterial infections.

This article presents the synthesis and comprehensive investigation of the static and dynamic magnetization properties of large-scale PEG-400-coated superparamagnetic Zn_xMn_1–xFe₂O₄ (0 ≤ × ≤ 0.8) nanoparticles, highlighting their potential use as magnetic carriers in magnetic fluid hyperthermia (MFH). The Rietveld analysis of the X-ray diffraction spectra confirmed that the surface-functionalized core nanoparticles exhibit a single-phase spinel structure at the nanoscale, ranging from 15.4 to 11.2 nm. The use of PEG-400 as a hydrophilic shell over Mn–Zn ferrite enhances colloidal stability, as evidenced by the elevated zeta potential (ζ) values ranging from −40 to −26 mV. This enhancement reflects an increase in electrostatic repulsion among Zn_xMn_1–xFe₂O₄ nanoparticles, making them well-suited for formulating viscoelastic and water-based magnetic fluids for hyperthermia applications. The AC inductive heating efficiency for magnetic hyperthermia was systematically investigated as a function of particle concentration, applied alternating magnetic field (AMF), and radiofrequency. To optimize hyperthermic performance, AMF strengths of 9.5 kA/m, 18.3 kA/m, and 25.4 kA/m were applied at corresponding constant frequencies of 586.4 kHz, 154.8 kHz, and 103 kHz, using nanoparticle concentrations of 3 and 6 mg/mL. The optimal heating performance, with maximum specific absorption rate and intrinsic loss power (ILP) values of 273.4 W/g and 5.271 nHm²/kg, respectively, was achieved at 18.3 kA/m and 154.8 kHz for a 3 mg/mL concentration, which was comparable to clinically approved magnetic hyperthermia fluids (ILP range: 0.15–3.1 nHm²/kg). At the highest tested frequency (586.4 kHz), the system deviated from linear response theory, further enhancing heating efficiency due to nonlinear Brownian and Neel relaxation processes. The Zn_xMn_1–xFe₂O₄ MNPs exhibit good biocompatibility (95–70% cell viability) with tested concentrations of 50, 100, 250, 500, and 1000 μM over HeLa cell lines.

Pathogenic bacterial infections, which can perpetuate a harmful cycle of inflammation and hinder wound healing. Consequently, constructing a multifunctional strategy that can both eradicate bacteria and alleviate excessive inflammation holds great significance for wound healing. Herein, this study developed multifunctional metal-phenolic nanopreparations (Quer-Fe NPs). Through the one-pot coordination of quercetin (Quer) and Fe, Quer-Fe NPs possess outstanding photothermal properties and reactive oxygen species scavenging capability. After the photothermal destruction of the biofilm, Quer-Fe NPs can ultimately exhibit good broad-spectrum antibacterial effects against Staphylococcus aureus (99.23%), Escherichia coli (90.34%), and Candida albicans (72.62%). RNA sequencing indicates that under the photothermal treatment of Quer-Fe NPs, it can interfere with the bacterial metabolic process and genetic material repair process, affect bacterial proliferation and biofilm diffusion, thereby achieving excellent antibacterial outcomes. Additionally, Quer-Fe NPs can also upregulate the anti-inflammatory genes and downregulate the pro-inflammatory genes in macrophages, and promote the polarization of macrophages from M1 to M2 to relieve inflammation. The in vivo wound healing treatment experiment demonstrates that this nanoformulation can accelerate the wound healing process. In this groundbreaking study, an ingeniously contrived minimalist methodology was formulated to synthesize multifunctional metal-phenolic nanozymes. These nanozymes incorporate highly efficacious photothermal antibacterial activity, bacterium-ensnaring capabilities, along with anti-inflammatory attributes, thereby spotlighting their prodigious potential in the remediation of bacterial infections.