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Examination of factors affecting condylar bone changes following surgical-orthodontic treatment. 研究影响外科正畸治疗后髁突骨变化的因素。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-03-01 Epub Date: 2022-09-13 DOI: 10.1080/08869634.2022.2118263
Satoshi Endo, Kanae Niimi, Yusuke Kato, Kaname Nohno, Daichi Hasebe, Takafumi Hayashi, Isao Saito, Tadaharu Kobayashi

Objective: To identify factors affecting condylar bone changes following surgical-orthodontic treatment.

Methods: A total of 200 patients with dentofacial deformities were classified into skeletal Classes I, II, and skeletal Class III groups consisting of 61 and 139 subjects, respectively. Temporomandibular joints (TMJs) were evaluated using clinical findings and computed tomography images before treatment, immediately before surgery, and 6 months after surgery.

Results: Condylar bone changes occurred at a significantly higher rate after surgery in both groups. Factors related to condylar bone changes following surgical-orthodontic treatment included skeletal Class I or II, disc displacement, and condylar bone changes before treatment. There were three cases with condylar bone changes after surgery that were diagnosed with condylar resorption and skeletal Class II and anterior disc displacement before surgery.

Conclusion: Condylar resorption could occur when the load on the condyle increases after orthognathic surgery and exceeds the permissible limit.

目的: 确定影响外科正畸治疗后髁突骨变化的因素:确定影响外科正畸治疗后髁突骨变化的因素:共将 200 名颌面部畸形患者分为骨骼Ⅰ类、Ⅱ类和骨骼Ⅲ类组,其中骨骼Ⅰ类组 61 人,Ⅱ类组 139 人。在治疗前、手术前和手术后 6 个月,使用临床结果和计算机断层扫描图像对颞下颌关节(TMJ)进行评估:结果:两组患者术后髁状突骨质变化的发生率均明显高于对照组。与手术正畸治疗后髁状突骨质改变相关的因素包括骨骼I级或II级、椎间盘移位以及治疗前的髁状突骨质改变。有三例手术后髁状突骨质改变的病例在手术前被诊断为髁状突吸收、骨骼Ⅱ级和前椎间盘移位:结论:当正颌手术后髁突上的负荷增加并超过允许限度时,可能会发生髁突吸收。
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引用次数: 0
Effects of Polyacrylic Acid with Different Molecular Weights on Stress Generation through Regulating the Growth of Calcium Carbonate within Collagen.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-28 DOI: 10.1021/acsabm.4c01878
Qianshen Tang, Jiawei Nie, Weijian Fang, Hao Xie, Weimin Wang, Hao Wang, Hang Ping, Bin Li, Zhengyi Fu

Mineralized collagen fibrils are the building blocks of bone, and the mineralization of collagen fibrils is generally regulated by noncollagenous proteins (NCPs). However, the functions of NCPs are difficult to investigate in vivo. Here, we use poly(acrylic acid) (PAA) with different molecular weights (5, 50, 450, and 4000 kDa) as analogs of NCPs and explore their effects on collagen mineralization in vitro. All the PAA molecules can promote the intrafibrillar mineralization of calcium carbonate (CaCO3) following these steps: the precursors infiltrate the gap zones of collagen, and transform into organized calcite nanocrystals within collagen. An increase in molecular weight significantly accelerates the mineralization rate of collagen films, approximately 0.67 μm min-1 at 4000 kDa, four times that of 5 kDa (0.16 μm min-1). However, the generation of contractile stress via intrafibrillar mineralization in tendons exhibits a contrary tendency. It reaches 24.2 MPa at 5 kDa, much higher than that of 4000 kDa (8.3 MPa), due to rapid mineralization causing severe extrafibrillar precipitation around the tendon. The controllable mineralization of collagen matrices may inspire the development of bone repair and regeneration in the future.

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引用次数: 0
Advancement of Nanomaterials- and Biomaterials-Based Technologies for Wound Healing and Tissue Regenerative Applications. 推进基于纳米材料和生物材料的伤口愈合和组织再生应用技术。
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-28 DOI: 10.1021/acsabm.5c00075
Durba Banerjee, Kalyan Vydiam, Venugopal Vangala, Sudip Mukherjee

Patients and healthcare systems face significant social and financial challenges due to the increasing number of individuals with chronic external and internal wounds that fail to heal. The complexity of the healing process remains a serious health concern, despite the effectiveness of conventional wound dressings in promoting healing. Recent advancements in materials science and fabrication techniques have led to the development of innovative dressings that enhance wound healing. To further expedite the healing process, novel approaches such as nanoparticles, 3D-printed wound dressings, and biomolecule-infused dressings have emerged, along with cell-based methods. Additionally, gene therapy technologies are being harnessed to generate stem cell derivatives that are more functional, selective, and responsive than their natural counterparts. This review highlights the significant potential of biomaterials, nanoparticles, 3D bioprinting, and gene- and cell-based therapies in wound healing. However, it also underscores the necessity for further research to address the existing challenges and integrate these strategies into standard clinical practice.

由于越来越多的人患有无法愈合的慢性外部和内部伤口,患者和医疗保健系统面临着巨大的社会和经济挑战。尽管传统伤口敷料能有效促进伤口愈合,但愈合过程的复杂性仍然是一个严重的健康问题。近年来,材料科学和制造技术的进步促使人们开发出了能促进伤口愈合的创新型敷料。为了进一步加快伤口愈合,出现了纳米粒子、3D 打印伤口敷料、生物分子注入敷料等新方法,以及基于细胞的方法。此外,基因治疗技术也被用来生成干细胞衍生物,这些衍生物比天然干细胞更具功能性、选择性和反应性。本综述强调了生物材料、纳米颗粒、三维生物打印以及基因和细胞疗法在伤口愈合方面的巨大潜力。不过,它也强调了进一步研究的必要性,以解决现有的挑战,并将这些策略纳入标准临床实践。
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引用次数: 0
Comparative Study of Fluorophores for Precise Dopamine Detection and Investigation of Its Association with Stress and Coffee Addiction in HEK 293 Cells.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-28 DOI: 10.1021/acsabm.5c00039
Ramakrishnan AbhijnaKrishna, Yueh-Hsun Lu, Shu-Pao Wu, Sivan Velmathi

In today's world, where stress and addiction are increasingly prevalent due to job pressures and coping mechanisms, dopamine (Dopa), a key hormone linked to mood, happiness, and mental health, has become vital for understanding conditions like depression and anxiety. Our study focuses on detecting Dopa pathways both in vitro using HEK293 cells and in vivo using zebrafish under stress and addiction conditions. We employed a biocompatible organic fluorophore (P1), with pyrazole-4-carboxaldehyde as the recognition unit, which demonstrated a detection limit of 8.2 nM, aligning with physiological Dopa levels. P1's efficacy in detecting Dopa was validated in human samples (urine, blood, and serum) and artificial samples, confirming its potential for real-world applications. This research is crucial for developing better diagnostic tools and therapies for dopamine-related disorders, offering significant societal benefits for addressing mental health challenges.

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引用次数: 0
Piezoelectricity Promotes 3D-Printed BTO/β-TCP Composite Scaffolds with Excellent Osteogenic Performance.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-27 DOI: 10.1021/acsabm.4c01754
Suyun Li, Yanbo Shan, Jingyi Chen, Ruyue Su, Lisheng Zhao, Rujie He, Ying Li

Piezoelectricity is reported to be able to promote bone scaffolds with excellent osteogenic performance. Herein, barium titanate/β-tricalcium phosphate (BTO/β-TCP) piezoelectric composite scaffolds were 3D printed, and their osteogenic performances were investigated in detail. The fabrication of BTO/β-TCP piezoelectric composite scaffolds employed cutting-edge DLP 3D printing technology. The scaffolds, featuring a triply periodic minimal surface (TPMS) design with a porosity of 60%, offered a unique structural framework. A comprehensive assessment of the composition, piezoelectric properties, and mechanical characteristics of the BTO/β-TCP scaffolds was conducted. Notably, an increase in the BTO volume fraction from 50 to 80 vol % within the scaffolds led to a reduction in compressive strength, decreasing from 2.47 to 1.74 MPa. However, this variation was accompanied by a substantial enhancement in the piezoelectric constant d33, soaring from 1.4 pC/N to 21.6 pC/N. Utilizing mouse osteoblasts (MC3T3-E1) in a live/dead cell staining assay, under the influence of external ultrasound, demonstrated the commendable biocompatibility of these piezoelectric composite ceramic bone scaffolds. Furthermore, thorough analyses of alkaline phosphatase (ALP) activity and polymerase chain reaction (PCR) findings provided compelling evidence of the scaffolds' superior osteogenic properties, underpinning their effectiveness at the cellular protein and gene levels. In conclusion, this study offers a groundbreaking strategy for the employment of BTO/β-TCP piezoelectric composite scaffolds in bone implant applications, harnessing their unique blend of biocompatibility, piezoelectricity, and osteogenic potential.

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引用次数: 0
Mucin Coatings Establish Multifunctional Properties on Commercial Sutures.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-27 DOI: 10.1021/acsabm.4c01793
Ufuk Gürer, Di Fan, Zhiyan Xu, Qaisar Nawaz, Jorrit Baartman, Aldo R Boccaccini, Oliver Lieleg

During the wound healing process, complications such as bacterial attachment or inflammation may occur, potentially leading to surgical site infections. To reduce this risk, many commercial sutures contain biocides such as triclosan; however, this chemical has been linked to toxicity and contributes to the occurrence of bacterial resistance. In response to the need for more biocompatible alternatives, we here present an approach inspired by the innate human defense system: utilizing mucin glycoproteins derived from porcine mucus to create more cytocompatible suture coatings with antibiofouling properties. By attaching manually purified mucin to commercially available sutures through a simple and rapid coating process, we obtain sutures with cell-repellent and antibacterial properties toward Gram-positive bacteria. Importantly, our approach preserves the very good mechanical and tribological properties of the sutures while offering options for further modifications: the mucin matrix can either be condensed for controlled localized drug release or covalently functionalized with inorganic nanoparticles for hard tissue applications, which allows for tailoring a commercial suture for specific biomedical use cases.

在伤口愈合过程中,可能会出现细菌附着或炎症等并发症,从而可能导致手术部位感染。为了降低这种风险,许多商用缝合线都含有三氯生等杀菌剂;然而,这种化学物质与毒性有关,而且会导致细菌产生抗药性。为了满足人们对更具生物相容性替代品的需求,我们在此介绍一种受人类先天防御系统启发的方法:利用从猪粘液中提取的粘蛋白糖蛋白来制造更具有细胞相容性和抗生物污染特性的缝合线涂层。通过简单快速的涂层工艺将人工纯化的粘蛋白附着在市售缝合线上,我们获得了对革兰氏阳性菌具有细胞排斥和抗菌特性的缝合线。重要的是,我们的方法保留了缝合线非常好的机械和摩擦学特性,同时还提供了进一步改性的选择:粘蛋白基质既可以凝结以控制局部药物释放,也可以与无机纳米粒子共价功能化以用于硬组织应用,从而为特定的生物医学用途定制商用缝合线。
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引用次数: 0
Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for Adventitious Capture of Cytoplasmic Proteins.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-27 DOI: 10.1021/acsabm.4c01621
Xiaobing Zuo, Alexander Jussupow, Nina S Ponomarenko, Thomas D Grant, Nicholas M Tefft, Neetu Singh Yadav, Kyleigh L Range, Corie Y Ralston, Michaela A TerAvest, Markus Sutter, Cheryl A Kerfeld, Josh V Vermaas, Michael Feig, David M Tiede

Bacterial microcompartments (BMCs) are self-assembling protein shell structures that are widely investigated across a broad range of biological and abiotic chemistry applications. A central challenge in BMC research is the targeted capture of enzymes during shell assembly. While crystallography and cryo-EM techniques have been successful in determining BMC shell structures, there has been only limited success in visualizing the location of BMC-captured enzyme cargo. Here, we demonstrate the opportunity to use small-angle X-ray scattering (SAXS) and pair distance distribution function (PDDF) measurements combined with quantitative comparison to coordinate structure models as an approach to characterize BMC shell structures in solution conditions directly relevant to biochemical function. Using this approach, we analyzed BMC shells from Haliangium ochraceum (HO) that were isolated following expression in E. coli. The analysis allowed the BMC shell structures and the extent of encapsulated enzyme cargo to be identified. Notably, the results demonstrate that HO-BMC shells adventitiously capture significant amounts of cytoplasmic cargo during assembly in E. coli. Our findings highlight the utility of SAXS/PDDF analysis for evaluating BMC architectures and enzyme encapsulation, offering valuable insights for designing BMC shells as platforms for biological and abiotic catalyst capture within confined environments.

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引用次数: 0
Characterization of a Bioactive Chitosan Dressing: A Comprehensive Solution for Different Wound Healing Phases.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-27 DOI: 10.1021/acsabm.4c01161
Hema Naveena A, Anup Kumar, Animesh Agrawal, Leo Mavely, Dhiraj Bhatia

Wound management has made significant advances over the past few decades, particularly with the development of advanced dressings that facilitate autolytic debridement, the absorption of wound exudate, and protection from external bacteria. However, finding a single dressing that effectively addresses all four phases of wound healing─hemostasis, inflammation, proliferation, and remodeling─remains a major challenge. Additionally, biofilms in chronic wounds pose a substantial obstacle by shielding microbes from topical antiseptics and antibiotics, thereby delaying the healing process. This study evaluates the wound-healing properties of a commercially available bioactive microfiber gelling (BMG) dressing made from chitosan alongside commercially available silver-loaded carboxymethyl cellulose (CMC-Ag) dressing, carboxymethyl cellulose dressing (CMC) and cotton gauze. In vitro testing demonstrated that the BMG dressing significantly exhibited superior fluid absorption and exudate-locking properties compared with the CMC-Ag dressing. Additionally, the BMG dressing effectively sequestered and eradicated wound-relevant pathogenic microorganisms, including drug-resistant bacteria. Its bioactive properties were further highlighted by its ability to enhance platelet-derived growth factor (PDGF) expression and sequester matrix metalloproteases (MMPs). Overall, this study highlights the effectiveness of the BMG dressing in wound management, particularly in exudate absorption and antimicrobial activity, demonstrating its relevance in wound care.

在过去的几十年里,伤口管理取得了长足的进步,尤其是先进敷料的开发,促进了自溶清创、伤口渗出物的吸收和外部细菌的防护。然而,找到一种能有效解决伤口愈合四个阶段(止血、炎症、增殖和重塑)的单一敷料仍是一大挑战。此外,慢性伤口中的生物膜也是一个巨大的障碍,它使微生物免受局部抗菌剂和抗生素的侵害,从而延缓了伤口的愈合过程。本研究评估了市售壳聚糖生物活性微纤维胶凝(BMG)敷料与市售含银羧甲基纤维素(CMC-Ag)敷料、羧甲基纤维素敷料(CMC)和棉纱布的伤口愈合特性。体外测试表明,与 CMC-Ag 敷料相比,BMG 敷料明显具有更好的液体吸收和渗出锁定性能。此外,BMG 敷料还能有效阻隔和消灭伤口相关的病原微生物,包括耐药菌。其生物活性特性还体现在它能增强血小板衍生生长因子(PDGF)的表达和封闭基质金属蛋白酶(MMPs)。总之,这项研究强调了 BMG 敷料在伤口管理方面的有效性,特别是在渗出物吸收和抗菌活性方面,证明了它在伤口护理方面的相关性。
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引用次数: 0
Biological Augmentation Using Electrospun Constructs with Dual Growth Factor Release for Rotator Cuff Repair.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-27 DOI: 10.1021/acsabm.4c02006
Yaping Ding, Yao Huang, Fucheng Zhang, Lei Wang, Wei Li, Hélder A Santos, Luning Sun

Surgical reattachment of tendon to bone is the standard therapy for rotator cuff tear (RCT), but its effectiveness is compromised by retear rates of up to 94%, primarily due to challenges in achieving successful tendon-bone enthesis regeneration under natural conditions. Biological augmentation using biomaterials has emerged as a promising approach to address this challenge. In this study, a bilayer construct incorporates polydopamine (PDA)-mediated bone morphogenetic protein 2 (BMP2) and BMP12 in separate poly(lactic-co-glycolic acid) (PLGA) fiber layers to promote osteoblast and tenocyte growth, respectively, and intermediate fibrocartilage formation, aiming to enhance the regenerative potential of tendon-bone interfaces. The lower layer, consisting of PLGA fibers with BMP2 immobilization through PDA adsorption, significantly accelerated osteoblast growth. Concurrently, the upper BMP12@PLGA-PDA fiber mat facilitated fibrocartilage formation and tendon tissue regeneration, evidenced by significantly elevated tenocyte viability and tenogenic differentiation markers. Therapeutic efficacy assessed through in vivo RCT models demonstrated that the dual-BMP construct augmentation significantly promoted the healing of tendon-bone interfaces, confirmed by biomechanical testing, cartilage immunohistochemistry analysis, and collagen I/II immunohistochemistry analysis. Overall, this combinational strategy, which combines augmentation patches with the controlled release of dual growth factors, shows great promise in improving the overall success rates of rotator cuff repairs.

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引用次数: 0
Collagen Hybridizing Peptides Promote Collagen Fibril Growth In Vitro.
IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2025-02-26 DOI: 10.1021/acsabm.4c01509
Sophia Huang, Nicole Ng, Mina Vaez, Boris Hinz, Iona Leong, Laurent Bozec

Recreating the structural and mechanical properties of native tissues in vitro presents significant challenges, particularly in mimicking the dense fibrillar network of extracellular matrixes such as skin and tendons. This study develops a reversible collagen film through cycling collagen self-assembly and disassembly, offering an innovative approach to address these challenges. We first generated an engineered collagen scaffold by applying plastic compression to the collagen hydrogel. The reversibility of the collagen assembly was explored by treating the scaffold with lactic acid, leading to its breakdown into an amorphous gel─a process termed defibrillogenesis. Subsequent immersion of this gel in phosphate buffer facilitated the reassembly of collagen into fibrils larger than those in the original scaffold yet with the D-banding pattern characteristic of collagen fibrils. Transfer learning of the mobileNetV2 convolutional neural network trained on atomic force microscope images of collagen nanoscale D-banding patterns was created with 99% training and testing accuracy. In addition, extensive external validation was performed, and the model achieved high robustness and generalization with unseen data sets. Further innovation was introduced by applying collagen hybridizing peptides, which significantly accelerated and directed the assembly of collagen fibrils, promoting a more organized and aligned fibrillar structure. This study not only demonstrates the feasibility of creating a reversible collagen film that closely mimics the density and structural properties of the native matrix but also highlights the potential of using collagen hybridizing peptides to control and enhance collagen fibrillogenesis. Our findings offer promising tissue engineering and regenerative medicine strategies by enabling precise manipulation of collagen structures in vitro.

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引用次数: 0
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