ACS Applied Bio Materials最新文献

In 2023, around 850 million people globally were affected by chronic kidney disease, which leads to the retention of uremic toxins and excess fluid in the blood. This study examines the adsorption of these toxins to poly(ethylene oxide) (PEO) films, known for their low-fouling properties. The gold surfaces were treated with 5 mM end-thiolated methoxy-terminated PEO (m-PEO) and analyzed using dynamic contact angle measurements, X-ray photoelectron spectroscopy, and spectroscopic ellipsometry to confirm the PEO film's presence and determine chain density. The adsorption of 25 different uremic toxins to m-PEO films was evaluated by using liquid chromatography-mass spectrometry (LC/MS), focusing on their binding affinity and adsorption dynamics. Results showed the effective modification of surfaces with m-PEO, with a notable change in contact angles and chain density (∼0.5 and 0.8 chains/nm²). Interestingly, pyruvic acid showed significant adsorption, whereas other toxins, such as hippuric acid, creatinine, and xanthosine had minimal interactions with the film. This indicates that the adsorption of these toxins is not primarily concentration driven and is rather dependent on the chemical structure of each toxin. These findings provide important insights for designing low-fouling coatings for biomedical devices.

Antibiotic misuse and bacterial resistance are pressing issues threatening public health. Natural plant extracts with bactericidal properties offer potential alternatives to reduce or replace antibiotic use. This study aims to develop a thermosensitive hydrogel containing daphnetin (DAP-TG) using poloxamers 407 (P407), polyvinylpyrrolidone (PVP), and poloxamers 188 (P188). We systematically evaluated the gel's antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as well as its antibacterial mechanisms. By examining the gelation temperature and time, degradation time, and in vitro release performance of DAP-TG, we produced a sustained-release DAP-TG with a rapid phase transition at (31.6 ± 0.1) °C. Its structure was characterized through Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The results indicated that the DAP thermosensitive hydrogel was formed and presented a 3D network spatial structure. The biocompatibility of DAP-TG was explored through the hemolysis test and cytotoxicity test. The results indicated that DAP-TG possessed excellent biocompatibility. The antibacterial efficacy of DAP-TG against E. coli and S. aureus was assessed using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curve, and inhibition zone tests. Results showed that DAP-TG exhibited excellent antibacterial activity against both E. coli and S. aureus, with MIC values of 1.28 and 0.32 mg/mL. The antibacterial mechanism of DAP-TG was preliminarily explored through the investigation of bacterial cell content leakage, AKP leakage, membrane permeability, SEM, ROS production, and biofilm inhibition activity. DAP-TG induced irreversible damage to the cell membranes of E. coli and S. aureus, resulting in enhanced permeability, elevated ROS levels, and inhibited biofilm formation. Our study indicates that DAP-TG exhibits effective sustained-release and antibacterial properties against E. coli and S. aureus in vitro, making it a promising candidate for antibacterial applications in food and pharmaceutical products.

Recent clinical studies have highlighted the presence of microclots in the form of amyloid fibrinogen particles (AFPs) in plasma samples from Long COVID patients. However, the clinical significance of these abnormal, nonfibrillar self-assembly aggregates of human fibrinogen remains debated due to the limited understanding of their structural and biological characteristics. In this study, we present a method for generating mimetic microclots in vitro. Using this approach, the self-assembly process, structural organization of AFPs, and their interactions with human plasma components were elucidated. The amyloid transition of fibrinogen occurs under acidic conditions within a pH range of 2.3-3.2. Well-dispersed amyloid oligomers of fibrinogen, ranging in size from 1 to 5 μm, can be prepared at pH 2.8 after 1 h of incubation. We tracked the dynamic self-assembly process at the single-molecule level using high-speed atomic force microscopy (HS-AFM). The arrangement of amyloid oligomers manifests as well-ordered, stacked nanodomains with striped patterns, growing perpendicular to the primary axis of the fibrinogen monomer. Upon transfer to physiological solution conditions or human plasma, these amyloid oligomers further aggregate into nonfibrillar structures at the micrometer scale, resembling the microclots observed in the bloodstream of Long COVID patients. Notably, these AFPs exhibit characteristics consistent with microclots, including positive staining in thioflavin T (ThT) assays and resistance to fibrinolysis. Proteomic analysis suggests that AFPs interact with various components of human plasma and have an enhanced binding affinity with complement C3 compared to native fibrinogen. This study enables the in vitro preparation of mimetic microclots exhibiting amyloid features. It is anticipated to facilitate further researches on the mechanisms, detection, and treatment of diseases associated with fibrinogen amyloidogenesis.

The current work presents the flame-retardant performance of hybrid polypropylene composites, reinforced with specific short woven flax fabrics (SWFs), short basalt fibers (BFs), and rice husk powder (RHP), using polypropylene grafted maleic anhydride (MAPP) as the coupling agent. Horizontal burning test (HBT), microcalorimeter test (MCT), and cone calorimeter test (CCT) were conducted on these composites. The formulations used were 25% SWF/PP, 25% SWF/20% BF/PP, and 25% SWF/20% BF/PP with 6% RHP and 25% SWF/20% BF/PP with varying RHP contents (6, 12, and 18%) in combination with 6% MAPP. A peak heat release rate (pHRR) reduction of 14.42% was recorded in microcalorimeter test results. However, cone calorimeter test results indicated a reduction of 80.95% in pHRR and 23.84% in total heat release rate (THR). Furthermore, the emissions of CO and CO₂ were reduced by 68 and 67%, respectively, in the 25SWF/20BF/PP.6MAPP-18RHP composite compared to pure PP, indicating an overall improvement in combustion efficiency. Fire performance index (FPI) also improved remarkably: FPI increased by 130% and fire growth index (FGI) improved by 81.34%. Moreover, the horizontal burning test showed an improved performance, with the burning rate further reduced by 11.10% compared to that of the 25% SWF/PP composite. These results highlight the synergy among natural fibers, agro-waste fillers, and MAPP in improving flame retardancy of polypropylene composites, and hence their potential application in automobiles and construction, where the demand for fire safety is very high.

The growing threat of bacterial resistance is a critical global health concern, necessitating the development of more efficient methods for evaluating antimicrobial efficacy. Here, we introduce a technique based on the sensitivity of bacterial collective motion to environmental changes, using motion trajectory analysis for swift antibiotic susceptibility appraisal within a simple spread-out of bacterial droplet. By single cell tracking in bacterial fluids near the droplet edge or boundary-detection of the colony expansion, we achieved rapid evaluation of antibiotic efficacy in under 60 min. This method is not only faster than traditional assays but also provides insights into drug-bacterial interactions, offering a powerful tool for advancing both diagnostic testing and the development of antimicrobial agents.

SARS-CoV-2 is a threat to global public health, which requires the development of safe measures to reduce the spread of this coronavirus. Herein, in this study, we prepared and examined potential antiviral agents based on ZnAl-layered double hydroxide (ZnAl-LDH) materials. ZnAl-LDH-based samples were synthesized via a one-pot low-temperature coprecipitation method, which features an ultrathin structure. The incorporation of trace amounts of Ag induces the formation of ZnO particles on the ZnAl-LDH surface, where both ZnO and Ag enhance UV light absorption. Interestingly, ZnAl-LDH-Ag shows a significantly high anticoronavirus effect upon exposure to the daylight lamp of the operation console and ultraviolet light. Moreover, ZnAl-LDH and ZnAl-LDH-Ag potently blocked the entry of SARS-CoV-2 pseudoparticles to cells. The in vivo biocompatibility experiment has demonstrated that ZnAl-LDH-Ag is a potentially biocompatible and potent anti-SARS-CoV-2 agent for virus prevention. The synergistic interactions between these nanoparticles continuously generate reactive oxygen species (ROS), leading to effective and sustained viral inactivation. This light-sensitive ROS production introduces a photocatalytic inactivation mechanism in antiviral materials. Moreover, unlike conventional antiviral agents that rapidly deplete their active components, the layered structure of this composite enables the controlled long-term release of antiviral radicals, enhancing its durability. ZnAl-LDH-Ag has been expected to be a promising solution for long-lasting antiviral applications.

The rapid emergence of multidrug-resistant (MDR) bacteria represents a critical global health threat, underscoring the urgent need for alternative antimicrobial strategies beyond conventional antibiotics. In this study, we report the synthesis of novel biobased antimicrobial polymers bearing quaternary ammonium salts, derived from sustainable feedstocks, maleic anhydride, dimethylaminobenzaldehyde, and furfurylamine. The functional tricyclic oxanorbornene lactam monomer is polymerized via ring opening metathesis polymerization, yielding well-defined polymers with controlled molar masses and low dispersity. Structural characterization is performed using 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, and the polymerization kinetics is monitored by online ¹H NMR spectroscopy. The quaternized biobased polymers demonstrate potent broad-spectrum antimicrobial activity against three clinically isolated MDR bacterial strains. They exhibit minimum inhibitory concentrations (MICs) that are significantly lower than those of several conventional antibiotics while also showing low hemolytic activity toward mammalian cells. This study highlights the potential of bioderived ROMP polymers as promising, sustainable antimicrobial polymers for combating the growing threat of antimicrobial resistance.

Chitosan is generally considered to be a procoagulant effect, which may cause adverse phenomena such as blood clotting when used in small-diameter vascular grafts. However, it also shows good biocompatibility and anti-inflammatory properties, which can facilitate vascular reconstruction. Therefore, it is significant to transition the effect of chitosan from coagulation promotion to antiplatelet while still harnessing its bioactivity. The procoagulant mechanism of chitosan is primarily attributed to the presence of protonated amino groups in the molecular chain. If the number of amino groups in chitosan is reduced, the procoagulant effect will be diminished as well. Aspirin has a strong antiplatelet function, and its molecular structure contains numerous active carboxyl groups, which can couple with the amino groups in chitosan. Aspirin-modified chitosan retains the biological activity of chitosan while also imparting an antiplatelet effect. In our study, we used a heparinized electrospun graft as the substrate and coated it with aspirin-modified chitosan to create a functional vascular graft. The blood clotting index of the graft remained above 80% after 45 min, and the platelet activation degree was only 4.03%. Additionally, the graft maintained complete patency with stable blood flow after 4 weeks of implantation and the vascular structure was largely rebuilt.

Skin wounds are extremely frequent injuries related to many etiologies. They are a burden on healthcare systems worldwide. Skin dressings are the most popular therapy, and collagen is the most commonly used biomaterial, although new sources of collagen have been studied, especially spongin-like from marine sponges (SPG), as a promising source due to a similar composition to vertebrates and the ability to function as a cell-matrix adhesion framework. Despite evidence showing the positive effects of SPG for tissue healing, the effects of skin dressings manufactured are still limited. In this context, this study aimed at investigating the effects of collagen skin dressings in an experimental model of skin wounds in rats. For this purpose, SEM, FTIR, cell viability, morphological and morphometric aspects, collagen deposition, and immunostaining of TGF-β and FGF were evaluated. The results demonstrated micro- and macropores on the rough surface, peak characteristics of collagen, and no cytotoxicity for the skin dressing. Also, the control group (CG) after 5 and 10 days exhibited an intense inflammatory process and the presence of granulation tissue, while the treated group (TG) exhibited re-epithelialization after 10 days. The evaluation of granulation tissue and neoepithelial length had an intragroup statistical difference (p = 0.0216) and no intergroup difference. Birefringence demonstrated an organized mesh arranged in a network pattern, presenting type I and type III collagen fibers in all groups. Moreover, in the morphometric evaluation, there were no statistical differences in intergroups or time points for the different types of collagen evaluated. In conclusion, these findings may indicate that the dressing has not exacerbated the inflammatory process and may allow faster healing. However, further studies using a critical wound healing injury model should be used, associated with longer experimental periods of evaluation, to further investigate the effects of these promising therapeutic approaches throughout the skin repair process.

The accumulation of oxidized low-density lipoprotein (oxLDL) in macrophages leads to the formation of foam cells and atherosclerosis development. Reducing the uptake of oxLDL in macrophages decreases the incidence and progression of atherosclerosis. Four distinct single-strand DNA sequences, namely, AP07, AP11, AP25, and AP29, were selected that demonstrated specific binding to distinct regions of oxidized apolipoprotein B100 (apoB100; the protein component of oxLDL) with low HDOCK scores. These four DNA sequences were combined to generate aptamers that selectively bound to labeled Dil-oxLDL, and were subsequently added to murine RAW 264.7 macrophages to test their inhibitory effects using fluorescence spectrometry. The four combined aptamers at 10 μM reduced oxLDL uptake by 79 ± 4% compared to that of the untreated aptamer group. Flow cytometry data demonstrated that macrophages treated with aptamers reached only 32.6% of the Dil-oxLDL signal, a 50% reduction in fluorescence emission relative to that of the untreated group (64.4% Dil-oxLDL signal). Binding the four combined aptamers to the oxLDL surface disrupted the interaction between oxLDL and CD36 via cyclic voltammetry, effectively decreasing the level of uptake of oxLDL by macrophages. Results suggested that these aptamers could be used as alternative compounds to prevent the formation of foam cells, hence providing antiatherosclerosis activity.