Pub Date : 2016-06-01DOI: 10.5798/DICLEMEDJ.0921.2016.02.0687
S. Erpolat, Zehra Arslanyılmaz, I. Kaygusuz
Objective: Acne vulgaris the most common cutaneous disorder affecting adolescent and young adults. Zinc is an essential element for human. We aimed to evaluate the serum zinc level in patients with acne vulgaris and correlate with the severity of disease. Methods: The study included 43 patients with acne vulgaris and 37 healthy control subjects. The severity of acne was assessed according to Global Acne Grading System. The serum zinc levels were measured both patients and control subjects. Results: The serum zinc level in acne patients were 81.48 ± 14.21 μg/dl and 83.69 ± 12.43 μg/dl in control subjects. There was no statistically significant difference between patients and control subjects (p=0.46).There was a negative correlation between acne severity and serum zinc levels but there was no statistically significant (r=-0.024, p=0.88). Conclusion: The results of our study revealed no relation between serum zinc levels and acne. Further studies are needed.
{"title":"Serum Zinc Levels in Patients with Acne Vulgaris and Its Correlation with Severity of Acne","authors":"S. Erpolat, Zehra Arslanyılmaz, I. Kaygusuz","doi":"10.5798/DICLEMEDJ.0921.2016.02.0687","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.02.0687","url":null,"abstract":"Objective: Acne vulgaris the most common cutaneous disorder affecting adolescent and young adults. Zinc is an essential element for human. We aimed to evaluate the serum zinc level in patients with acne vulgaris and correlate with the severity of disease. Methods: The study included 43 patients with acne vulgaris and 37 healthy control subjects. The severity of acne was assessed according to Global Acne Grading System. The serum zinc levels were measured both patients and control subjects. Results: The serum zinc level in acne patients were 81.48 ± 14.21 μg/dl and 83.69 ± 12.43 μg/dl in control subjects. There was no statistically significant difference between patients and control subjects (p=0.46).There was a negative correlation between acne severity and serum zinc levels but there was no statistically significant (r=-0.024, p=0.88). Conclusion: The results of our study revealed no relation between serum zinc levels and acne. Further studies are needed.","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"310-314"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0628
Monika Gupta, S. Malik, R. Kalra, N. Sharma, Sunita Singh, Veena Gupta, P. Jain, R. Sen
Amac: Son yillarda rutin kemik iligi aspirati incelemelerinde saptanan neoplastik ve non-neoplastik hastaliklarda giderek artan oranlarda plazma hucrelerine rastlanmaktadir. Bu calismada kemik iligi aspiratinda plazmasitoz saptanan durumlarin bir spektrumu cikarilmaya calisilmistir.Yontemler: Sunulan calisma bir yillik sure icinde Patoloji Bolumunde gerceklestirildi. Calisma materyalini artmis oranda plazma hucresi (>%3,5) gosteren toplam 114 kemik iligi aspirati olusturmaktadir. Tum olgularda ayrintili bir klinik muayene ve takiben tam kan sayimi, periferik yayma ve kemik iligi aspirasyonu yapildi.Bulgular: Erkek / Kadin orani acisindan hafif bir kadin ustunlugu goruldu (1 / 1,1). Vakalarin cogu 40’li yaslarda idi. Plazma hucre yogunlugu %5-36 arasinda degismekte idi. Etyoloji acisindan 96 (%84,2) olguda non-neoplastik, 18 (%15,8) olguda ise neoplastik bir etyoloji saptandi.Sonuc: Kemik iligi plazmasitozu tanisal bir karmasaya yol acarak, ozellikle sayi ve morfolojik gorunumlerinde cakisma olan reaktif patolojilerin neoplastik durumlardan ayirt edilmesini bazen zorlastirabilir. Ozellikle cakisma araliklarindakiler basta olmak uzere, plazmasitozlu her hastada bireysellestirilmis arastirmalar ve immunelektroforez ve immunhistokimya ile boyanmis kemik iligi aspirati incelemesi ile sonuc tanisi konabilir ve hasta yonetimi gerceklestirilebilir
{"title":"Etiological Profile of Plasmacytosis on Bone Marrow Aspirates","authors":"Monika Gupta, S. Malik, R. Kalra, N. Sharma, Sunita Singh, Veena Gupta, P. Jain, R. Sen","doi":"10.5798/DICLEMEDJ.0921.2016.01.0628","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0628","url":null,"abstract":"Amac: Son yillarda rutin kemik iligi aspirati incelemelerinde saptanan neoplastik ve non-neoplastik hastaliklarda giderek artan oranlarda plazma hucrelerine rastlanmaktadir. Bu calismada kemik iligi aspiratinda plazmasitoz saptanan durumlarin bir spektrumu cikarilmaya calisilmistir.Yontemler: Sunulan calisma bir yillik sure icinde Patoloji Bolumunde gerceklestirildi. Calisma materyalini artmis oranda plazma hucresi (>%3,5) gosteren toplam 114 kemik iligi aspirati olusturmaktadir. Tum olgularda ayrintili bir klinik muayene ve takiben tam kan sayimi, periferik yayma ve kemik iligi aspirasyonu yapildi.Bulgular: Erkek / Kadin orani acisindan hafif bir kadin ustunlugu goruldu (1 / 1,1). Vakalarin cogu 40’li yaslarda idi. Plazma hucre yogunlugu %5-36 arasinda degismekte idi. Etyoloji acisindan 96 (%84,2) olguda non-neoplastik, 18 (%15,8) olguda ise neoplastik bir etyoloji saptandi.Sonuc: Kemik iligi plazmasitozu tanisal bir karmasaya yol acarak, ozellikle sayi ve morfolojik gorunumlerinde cakisma olan reaktif patolojilerin neoplastik durumlardan ayirt edilmesini bazen zorlastirabilir. Ozellikle cakisma araliklarindakiler basta olmak uzere, plazmasitozlu her hastada bireysellestirilmis arastirmalar ve immunelektroforez ve immunhistokimya ile boyanmis kemik iligi aspirati incelemesi ile sonuc tanisi konabilir ve hasta yonetimi gerceklestirilebilir","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71065999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0630
Çiğdem Canbolat Seyman, S. Ayaz
Objectives: Patient and staff safety is a crucial issue for the health agenda of all countries. Patient safety involves promoting the measures which ensure that mistakes are noted, reported and corrected before they affect patients and health workers. This descriptive study was conducted to determine the opinions of operating room nurses regarding patient and staff safety, as well as the factors that affect these opinions. Methods: This descriptive study was conducted in 2010 in the operating rooms of nine quality-certified public hospitals in Ankara. This study was applied to 100 operating room nurses. Data was collected through questionnaires. Results: Operating room nurses report that the operating room engenders many risks towards patient and staff safety. The study shows that the three most frequent factors which threaten patient safety are: risk of infection (50%), problems with the transport of patients (50%), and wrong-patient/wrong-side/wrong-operation (35%). This study also revealed that the threats to staff safety are: sharp and penetrating injuries (44%), exposure to diseases that spread through contact with blood, body fluid, or respiration (41%). Conclusion: Nurses reported a multitude of risks that affect patient and staff safety in the operating room. The most important of these are risk of infection and sharp, penetrating injuries. Key words: Operating room, patient and staff safety, nursing
{"title":"Opinions of Operating Room Nurses Regarding Patient and Staff Safety in Operating Room","authors":"Çiğdem Canbolat Seyman, S. Ayaz","doi":"10.5798/DICLEMEDJ.0921.2016.01.0630","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0630","url":null,"abstract":"Objectives: Patient and staff safety is a crucial issue for the health agenda of all countries. Patient safety involves promoting the measures which ensure that mistakes are noted, reported and corrected before they affect patients and health workers. This descriptive study was conducted to determine the opinions of operating room nurses regarding patient and staff safety, as well as the factors that affect these opinions. Methods: This descriptive study was conducted in 2010 in the operating rooms of nine quality-certified public hospitals in Ankara. This study was applied to 100 operating room nurses. Data was collected through questionnaires. Results: Operating room nurses report that the operating room engenders many risks towards patient and staff safety. The study shows that the three most frequent factors which threaten patient safety are: risk of infection (50%), problems with the transport of patients (50%), and wrong-patient/wrong-side/wrong-operation (35%). This study also revealed that the threats to staff safety are: sharp and penetrating injuries (44%), exposure to diseases that spread through contact with blood, body fluid, or respiration (41%). Conclusion: Nurses reported a multitude of risks that affect patient and staff safety in the operating room. The most important of these are risk of infection and sharp, penetrating injuries. Key words: Operating room, patient and staff safety, nursing","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0644
S. Hot, N. Duraker, A. Sarı, Kenan Çetin
Objective: Because of its poor prognosis and high mortality rate, early diagnosis of acute mesenteric ischemia (AMI) is a challenge. The value of serum markers in the diagnosis of AMI is limited and the studies related with the D-dimer test carried out in recent years. In this prospective study we investigated the value of the level of plasma D-dimer in the diagnosis of AMI and the differential diagnosis from acute pancreatitis and acute cholecystitis. Methods: We included 89 patients who admitted to our emergency department with abdominal pain. The number and the diagnosis of the patients were 17 AMI, 42 acute pancreatitis and 30 acute cholecystitis, respectively. We measured the levels of plasma D-dimer of all patients by using a latex agglutination ‘immunoassay’ method. Eventually we calculated the sensitivity and specificity of D-dimer test in the diagnosis of AMI. Results: We determined the specificity of the D-dimer test in the differential diagnosis of AMI as 50% from acute pancreatitis, 70% from acute cholecystitis, 58.3% from all the control group and the sensitivity was 100%. Conclusion: The measurement of plasma D-dimer concentration may be useful in the differential diagnosis of AMI from acute pancreatitis and acute cholecystitis. However, to reveal the diagnostic value of D-dimer test more clearly, further studies with larger series are needed, where cut-off value is highly defined, and other patients with acute abdominal pain are added into the control group. Key words: Acute mesenteric ischemia, diagnosis, D-dimer
{"title":"The Value of D-Dimer in Diagnosis of Acute Mesenteric Ischemia and Differential Diagnosis from Acute Pancreatitis and Acute Cholecystitis","authors":"S. Hot, N. Duraker, A. Sarı, Kenan Çetin","doi":"10.5798/DICLEMEDJ.0921.2016.01.0644","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0644","url":null,"abstract":"Objective: Because of its poor prognosis and high mortality rate, early diagnosis of acute mesenteric ischemia (AMI) is a challenge. The value of serum markers in the diagnosis of AMI is limited and the studies related with the D-dimer test carried out in recent years. In this prospective study we investigated the value of the level of plasma D-dimer in the diagnosis of AMI and the differential diagnosis from acute pancreatitis and acute cholecystitis. Methods: We included 89 patients who admitted to our emergency department with abdominal pain. The number and the diagnosis of the patients were 17 AMI, 42 acute pancreatitis and 30 acute cholecystitis, respectively. We measured the levels of plasma D-dimer of all patients by using a latex agglutination ‘immunoassay’ method. Eventually we calculated the sensitivity and specificity of D-dimer test in the diagnosis of AMI. Results: We determined the specificity of the D-dimer test in the differential diagnosis of AMI as 50% from acute pancreatitis, 70% from acute cholecystitis, 58.3% from all the control group and the sensitivity was 100%. Conclusion: The measurement of plasma D-dimer concentration may be useful in the differential diagnosis of AMI from acute pancreatitis and acute cholecystitis. However, to reveal the diagnostic value of D-dimer test more clearly, further studies with larger series are needed, where cut-off value is highly defined, and other patients with acute abdominal pain are added into the control group. Key words: Acute mesenteric ischemia, diagnosis, D-dimer","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"88-92"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0635
H. Celik, A. camtosun, C. Ediz, İ. Topçu, R. Altıntaş, C. Taşdemir
Amac: Calismanin amaci, tas hastaliginin endemik oldugu ve sik ureterorenoskopi yapilan iki farkli sehirdeki universite hastanesi ve devlet hastanesinde yapilan ureterorenoskopik litotripsi sonrasi ureteral stent yerlestirme sikliginin karsilastirilmasidir.Yontemler: Iki bin on dort Ocak ayi ile 2014 Mayis tarihleri arasinda Inonu univeritesi Turgut Ozal Tip Merkezi Hastanesi (TOTM) ve Osmaniye Devlet Hastanesi (ODH) uroloji kliniklerine basvuran hastalar retrospektif olarak degerlendirildi. Ureteral tasa bagli olarak ureteroskopik litotripsi yapilan hastalar tas lokalizasyonu, boyutu, ektazi dereceleri ve stent yerlestirilme durumuna gore degerlendirildi.Bulgular: Her iki hastaneden 92 ser hasta calismaya alindi. Endoskopik ureteral tas tedavisi sonrasi TOTM’de 85 ve ODH’de 82 hastaya DJ stent yerlestirildi. Universite hastanesinde stent yerlestirilme sikligi devlet hastanesinde yuksek ciksa da istatiksel anlamlilik yoktu. Her iki grup arasinda ortalama operasyon sureleri arasinda istatiksel anlamlilik vardi.Sonuc: Endoskopik ureter tasi tedavisi sonrasi DJ stent yerlestirilmesi cok sik uygulanmaktadir. Bizim calismamiza gore universite hastanelerinde devlet hastanelerine gore daha sik DJ stent yerlestirilmesi yapilmaktadir. Bunun nedeni universite hastanelerinin son basamak merkezler olmasindan dolayi daha komplike hastalarin bu merkezlere refere edilmesi olabilir. Bu durumun daha net aydinlatilabilmesi icin, prospektif, cok merkezli ve daha genis serili calismalara ihtiyac duyulmaktadir
阿马克：Calisman的目标是诱发这种疾病，以及两所不同的魔法医院和州立医院的输尿管镜结石，它们都内置在尿道支架中。联系方式：2014年1月2日，伊诺大学图尔古特-奥扎尔中心医院（TOTM）和奥斯曼尼耶州立医院（ODH）的神经科诊所对开了2000块馅饼的患者进行了回顾。输尿管镜结石型患者被部署到准确的位置、大小、摇头丸水平和支架部署。保加利亚：两家医院的92名患者被感染。对85例TOTM和82例ODH患者采用DJ支架内窥镜尿道治疗。如果州立医院的支架很高，那么在大学医院没有统计学意义。在两组之间，平均手术图像之间具有统计学意义。结果：DJ支架置入后尿道组织内窥镜治疗完成。Bizim calimamiza gore universite hastanelerine devlet hastanelenine gore daha sik DJ支架yerlestirilmesi yapilmaktadir。这就是为什么更复杂的患者可以转诊到这些中心，因为它们是大学医院的最后一个中心。为了澄清这一情况，招股说明书需要听到更集中、更连续的口号。
{"title":"Frequency of Stent Placement after Ureteroscopic Lithotripsy in a University and a State Hospital","authors":"H. Celik, A. camtosun, C. Ediz, İ. Topçu, R. Altıntaş, C. Taşdemir","doi":"10.5798/DICLEMEDJ.0921.2016.01.0635","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0635","url":null,"abstract":"Amac: Calismanin amaci, tas hastaliginin endemik oldugu ve sik ureterorenoskopi yapilan iki farkli sehirdeki universite hastanesi ve devlet hastanesinde yapilan ureterorenoskopik litotripsi sonrasi ureteral stent yerlestirme sikliginin karsilastirilmasidir.Yontemler: Iki bin on dort Ocak ayi ile 2014 Mayis tarihleri arasinda Inonu univeritesi Turgut Ozal Tip Merkezi Hastanesi (TOTM) ve Osmaniye Devlet Hastanesi (ODH) uroloji kliniklerine basvuran hastalar retrospektif olarak degerlendirildi. Ureteral tasa bagli olarak ureteroskopik litotripsi yapilan hastalar tas lokalizasyonu, boyutu, ektazi dereceleri ve stent yerlestirilme durumuna gore degerlendirildi.Bulgular: Her iki hastaneden 92 ser hasta calismaya alindi. Endoskopik ureteral tas tedavisi sonrasi TOTM’de 85 ve ODH’de 82 hastaya DJ stent yerlestirildi. Universite hastanesinde stent yerlestirilme sikligi devlet hastanesinde yuksek ciksa da istatiksel anlamlilik yoktu. Her iki grup arasinda ortalama operasyon sureleri arasinda istatiksel anlamlilik vardi.Sonuc: Endoskopik ureter tasi tedavisi sonrasi DJ stent yerlestirilmesi cok sik uygulanmaktadir. Bizim calismamiza gore universite hastanelerinde devlet hastanelerine gore daha sik DJ stent yerlestirilmesi yapilmaktadir. Bunun nedeni universite hastanelerinin son basamak merkezler olmasindan dolayi daha komplike hastalarin bu merkezlere refere edilmesi olabilir. Bu durumun daha net aydinlatilabilmesi icin, prospektif, cok merkezli ve daha genis serili calismalara ihtiyac duyulmaktadir","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"39-42"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0634
B. Çimen, B. Saraymen, I. Çetin, Mustafa Yavuz Köker, A. Cetin, B. Eser
Amac: Duyarlilik acisindan sinirli olan immunolojik yontemler kullanilarak ozellikle normal dokulardaki P-glikoprotein ekpresyonunun belirlenmesi sorun olusturmaktadir. Bu calismada hematolojik malignansi tanisi almis ve tedaviye yanit vermeyen hastalarda P-glikoprotein ve CD34 ekpresyonunun degerlendirilmesi icin, P-glikoprotein and CD34 ekpresyonunun akim sitometri ile belirlenmesini amacladik.Yontemler: Erciyes Universitesi Mehmet-Kemal Dedeman Hematoloji-Onkoloji Hastanesine basvuran akut miyeloblastik losemi ile akut lenfoblastik losemi tanisi almis 50 hasta ve yirmi kontrol calismaya dâhil edildi. Hastalarin kemik iligi ornekleri ile kontrol grubundan alinan periferik kan orneklerinden elde adilen suspanse hucreler, P-glikoprotein fikoeritrin ile ve CD34 FITC ya da PerCP Cy 5.5 antikorlari ile isaretlendi ve daha sonra yuzey ekspresyonlari akim sitometri ile olculdu.Bulgular: Calismamizda 30 akut miyeloblastik losemi hastasinin 6’sinda (%20) P-glikoprotein ve CD34 ekspresyonu tespit edilirken, 20 akut lenfoblastik losemi hastasinin 6’sinda (%30) P-glikoprotein, 5’inde (%25) CD34 ekspresyonu tespit edildi. Akut miyeloblastik losemi ve akut lenfoblastik losemi kemik iligi orneklerinde P-glikoprotein ve CD34 ekspresyonlari arasinda anlamli bir iliski oldugu goruldu. Sonuc: Bulgular, akim sitometri yontemi ile P-glikoprotein ekspresyonunun akut miyeloblastik losemi ve akut lenfoblastik losemi kemik iligi orneklerinde molekuler yontemlerden daha hizli, guvenilir ve dogru bir sekilde olculebildigini, P-glikoprotein ile CD34 ekspresyonlari arasinda anlamli bir iliski olabilecegini gostermistir. Yuzeyinde CD34 eksprese eden blast hucrelerinin ayni zamanda P-glikoprotein eksprese etmeleri hucrelerdeki coklu ilac direnci geninin daha cok olgunlasmamis hucrelerde aktif oldugunu gostermektedir
Amac：使用对感觉酸感到愤怒的免疫化合物，很难检测正常组织中的P-糖蛋白压缩。在那些对血液系统恶性肿瘤没有反应的患者中，我们的目标是减少对治疗没有反应的病人的P-糖蛋白和CD34按压，并确定P-糖蛋白和CD4按压的确切谷值。Yontemler：埃尔西耶大学的Mehmet Kemal Dedeman Hematoloji Onkoloji Hastanesine在50岁的时候就已经控制好了。患者暴露于骨髓细胞和对照组的血细胞，然后是外周血细胞、P-糖蛋白固定蛋白和CD34-FITC或PerCP-Cy 5.5抗凝剂，随后是急性谷值测定的高表达。在calimamide中，在6（20）P-糖蛋白和CD34表达中检测到30例急性成髓细胞损失，在6、25和CD34中检测到20例急性成粒细胞损失。在P-糖蛋白和CD34表达之间的骨髓装饰物中观察到急性成髓细胞损失和急性成栓细胞损失。结果：肿胀是一种酸性谷蛋白测定法，具有急性成髓细胞性P-糖蛋白表达缺失和骨髓装饰物的急性lenfoblasty缺失，鹅膏病分子在P-糖蛋白和CD34表达之间的序列可以更快、更肥胖和更正确。同时，南斯拉夫展示CD34爆炸物的爆破细胞也表明，P-糖蛋白在细胞中总耐药性增加的细胞中具有活性。
{"title":"Determination of P-Glycoprotein Expression by Flow Cytometry in Hematological Malignancies","authors":"B. Çimen, B. Saraymen, I. Çetin, Mustafa Yavuz Köker, A. Cetin, B. Eser","doi":"10.5798/DICLEMEDJ.0921.2016.01.0634","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0634","url":null,"abstract":"Amac: Duyarlilik acisindan sinirli olan immunolojik yontemler kullanilarak ozellikle normal dokulardaki P-glikoprotein ekpresyonunun belirlenmesi sorun olusturmaktadir. Bu calismada hematolojik malignansi tanisi almis ve tedaviye yanit vermeyen hastalarda P-glikoprotein ve CD34 ekpresyonunun degerlendirilmesi icin, P-glikoprotein and CD34 ekpresyonunun akim sitometri ile belirlenmesini amacladik.Yontemler: Erciyes Universitesi Mehmet-Kemal Dedeman Hematoloji-Onkoloji Hastanesine basvuran akut miyeloblastik losemi ile akut lenfoblastik losemi tanisi almis 50 hasta ve yirmi kontrol calismaya dâhil edildi. Hastalarin kemik iligi ornekleri ile kontrol grubundan alinan periferik kan orneklerinden elde adilen suspanse hucreler, P-glikoprotein fikoeritrin ile ve CD34 FITC ya da PerCP Cy 5.5 antikorlari ile isaretlendi ve daha sonra yuzey ekspresyonlari akim sitometri ile olculdu.Bulgular: Calismamizda 30 akut miyeloblastik losemi hastasinin 6’sinda (%20) P-glikoprotein ve CD34 ekspresyonu tespit edilirken, 20 akut lenfoblastik losemi hastasinin 6’sinda (%30) P-glikoprotein, 5’inde (%25) CD34 ekspresyonu tespit edildi. Akut miyeloblastik losemi ve akut lenfoblastik losemi kemik iligi orneklerinde P-glikoprotein ve CD34 ekspresyonlari arasinda anlamli bir iliski oldugu goruldu. Sonuc: Bulgular, akim sitometri yontemi ile P-glikoprotein ekspresyonunun akut miyeloblastik losemi ve akut lenfoblastik losemi kemik iligi orneklerinde molekuler yontemlerden daha hizli, guvenilir ve dogru bir sekilde olculebildigini, P-glikoprotein ile CD34 ekspresyonlari arasinda anlamli bir iliski olabilecegini gostermistir. Yuzeyinde CD34 eksprese eden blast hucrelerinin ayni zamanda P-glikoprotein eksprese etmeleri hucrelerdeki coklu ilac direnci geninin daha cok olgunlasmamis hucrelerde aktif oldugunu gostermektedir","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"45 1","pages":"33-38"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/DICLEMEDJ.0921.2016.01.0647
H. Alptekin, N. Alptekin, H. Işık, Y. Tanrıkulu, E. Şahin
Objective: Brain derived neurotrophic factor (BDNF) is the most important neurotrophin, which helps the differentiation and growth of central and peripheral neurons, and facilitates synaptic transmission. In this study we aimed to investigate fetal cord BDNF levels of infants born from subclinical and clinical maternal hypothyroidism. Methods: This study was conducted on a total of 67 pregnant women who were followed up in Obstetrics and Gynecology outpatient clinics, 27 with maternal hypothyroidism and 40 age-parity matched healthy pregnants without hypothyroidism. Immediately after vaginal or cesarean delivery fetal cord blood samples were taken from these patients and BDNF levels were measured. Results: BDNF levels of infants born from pregnants with maternal hypothyroidism were significantly lower than the control group (23.3 ± 17.4 ng/dl and 50.7 ± 28.3 ng/dl respectively; p<0.001). In multiple linear regression analysis, while BDNF level was related with maternal hypothyroidism and infant sex, it was not associated with mode of delivery, maternal age, total weight gain during pregnancy, gestational age at birth, thyroid stimulating hormone (TSH) levels and other neonatal data. Conclusion: This study showed that fetal cord BDNF levels significantly decreased in infants of the pregnants with hypothyroidism. Key words: Pregnancy, maternal hypothyroidism, brain derived neurotrophic factor, levothyroxine
{"title":"The Effect of Maternal Hypothyroidism on Fetal Umbilical Cord Brain-Derived Neurotrophic Factor Levels","authors":"H. Alptekin, N. Alptekin, H. Işık, Y. Tanrıkulu, E. Şahin","doi":"10.5798/DICLEMEDJ.0921.2016.01.0647","DOIUrl":"https://doi.org/10.5798/DICLEMEDJ.0921.2016.01.0647","url":null,"abstract":"Objective: Brain derived neurotrophic factor (BDNF) is the most important neurotrophin, which helps the differentiation and growth of central and peripheral neurons, and facilitates synaptic transmission. In this study we aimed to investigate fetal cord BDNF levels of infants born from subclinical and clinical maternal hypothyroidism. Methods: This study was conducted on a total of 67 pregnant women who were followed up in Obstetrics and Gynecology outpatient clinics, 27 with maternal hypothyroidism and 40 age-parity matched healthy pregnants without hypothyroidism. Immediately after vaginal or cesarean delivery fetal cord blood samples were taken from these patients and BDNF levels were measured. Results: BDNF levels of infants born from pregnants with maternal hypothyroidism were significantly lower than the control group (23.3 ± 17.4 ng/dl and 50.7 ± 28.3 ng/dl respectively; p<0.001). In multiple linear regression analysis, while BDNF level was related with maternal hypothyroidism and infant sex, it was not associated with mode of delivery, maternal age, total weight gain during pregnancy, gestational age at birth, thyroid stimulating hormone (TSH) levels and other neonatal data. Conclusion: This study showed that fetal cord BDNF levels significantly decreased in infants of the pregnants with hypothyroidism. Key words: Pregnancy, maternal hypothyroidism, brain derived neurotrophic factor, levothyroxine","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"43 1","pages":"107-111"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.5798/diclemedj.0921.2016.01.0663
A. Kaydu, Erhan Gökçek, Cem Kıvılcım Kaçar, M. Kuyumcu
Lumbar caudal epidural steroid injection (LKES ) is one of the non-surgical treatment of back pain. In this case report; we presented persistent hiccup after lomber caudal epidural steroid injection for chronic low back pain and treatment of this case. Key words: Steroid, persistent hiccup, lomber injection, low back pain
{"title":"A Rare Complication after Caudal Epidural Steroid Injection: Persistent Hiccup","authors":"A. Kaydu, Erhan Gökçek, Cem Kıvılcım Kaçar, M. Kuyumcu","doi":"10.5798/diclemedj.0921.2016.01.0663","DOIUrl":"https://doi.org/10.5798/diclemedj.0921.2016.01.0663","url":null,"abstract":"Lumbar caudal epidural steroid injection (LKES ) is one of the non-surgical treatment of back pain. In this case report; we presented persistent hiccup after lomber caudal epidural steroid injection for chronic low back pain and treatment of this case. Key words: Steroid, persistent hiccup, lomber injection, low back pain","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"97 1","pages":"174-176"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71066152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-01DOI: 10.5798/diclemedj.0921.2015.04.0610
Muammer Bilici, I. Oz, S. Ilikhan, Müzeyyen Arslaner, E. Kahraman, B. Kılavuz, Zeynep Özdamar, Ş. Ertop
Objective: In the present study, we aimed to consider the relation between the manifestations of venous thromboembolism (VTE) and gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T. Methods: One hundred and forty four patients with idiopathic VTE were enrolled in this study. The data of patients were obtained from the medical records in hospital information system. The patients were grouped according to the location of VTE. In all subjects FVL, prothrombin G20210A, and MTHFR C677T were analyzed by specific polymerase chain reactions and restriction enzymes. Univariate and multivariate analysis were used to evaluate the relation between the groups and the gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T. Results: The mean age of patients was 41.16 ± 13.23 years and the male / female ratio was 1.18. Among the patients with VTE, 44 (30.6%) had only DVT, 41 (28.5%) had only PE, 26 (18.1%) had both DVT and PE, 23 (16%) had cerebral veins thrombosis (CVT) and 10 (6.9%) had abdominal vein thrombosis The prevalence was found to be 46.5% for FVL, 13.2% for prothrombin G20210A and 45.1% for MTHFR C677T gene mutation among patients. There was no statistically difference between the manifestations of VTE regarding the gene mutations (p>0,05). Conclusion: The findings of this study suggest that gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T are not sufficient to determine the location of VTE. Key words: Venous thromboembolism; factor V Leiden; prothrombin G20210A; MTHFR C677T
{"title":"The predictive value of factor V Leiden, prothrombin G20210A and MTHFR C677T Gene mutations on the location of venous thromboembolism","authors":"Muammer Bilici, I. Oz, S. Ilikhan, Müzeyyen Arslaner, E. Kahraman, B. Kılavuz, Zeynep Özdamar, Ş. Ertop","doi":"10.5798/diclemedj.0921.2015.04.0610","DOIUrl":"https://doi.org/10.5798/diclemedj.0921.2015.04.0610","url":null,"abstract":"Objective: In the present study, we aimed to consider the relation between the manifestations of venous thromboembolism (VTE) and gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T. Methods: One hundred and forty four patients with idiopathic VTE were enrolled in this study. The data of patients were obtained from the medical records in hospital information system. The patients were grouped according to the location of VTE. In all subjects FVL, prothrombin G20210A, and MTHFR C677T were analyzed by specific polymerase chain reactions and restriction enzymes. Univariate and multivariate analysis were used to evaluate the relation between the groups and the gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T. Results: The mean age of patients was 41.16 ± 13.23 years and the male / female ratio was 1.18. Among the patients with VTE, 44 (30.6%) had only DVT, 41 (28.5%) had only PE, 26 (18.1%) had both DVT and PE, 23 (16%) had cerebral veins thrombosis (CVT) and 10 (6.9%) had abdominal vein thrombosis The prevalence was found to be 46.5% for FVL, 13.2% for prothrombin G20210A and 45.1% for MTHFR C677T gene mutation among patients. There was no statistically difference between the manifestations of VTE regarding the gene mutations (p>0,05). Conclusion: The findings of this study suggest that gene mutations including factor V Leiden (FVL), prothrombin G20210A and MTHFR C677T are not sufficient to determine the location of VTE. Key words: Venous thromboembolism; factor V Leiden; prothrombin G20210A; MTHFR C677T","PeriodicalId":30104,"journal":{"name":"Dicle Medical Journal","volume":"42 1","pages":"467-471"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71065812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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