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Impact of Adolescent Social Isolation on Adult, Binge-Like Ethanol Consumption and Plasma Corticosterone in High-Alcohol-Preferring Mice 青少年社会隔离对高酒精偏好小鼠成年后酒精消耗和血浆皮质酮的影响
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1563
Eva Cullins
Adolescent stress exposure increases the likelihood of alcohol misuse and alcohol use disorder (AUD) in adulthood; however, it is not clear how genetic and environmental factors interact to increase risk. Th is study examined how adolescent social isolation aff ects adult binge- like ethanol drinking and levels of the stress hormone corticosterone in male and female mice with a genetic predisposition toward high alcohol preference (HAP). Twenty- eight HAP mice were separated into group- housed (GH) and socially isolated (SI) conditions ( n = 13, 13). Binge drinking was assessed using a drinking in the dark (DID) procedure. Blood samples were taken before DID and aft er the 4th (last) day of DID. Overall, adolescent social isolation increased adult binge drinking in a sex- and time- dependent manner. Analysis of Hour 1 intake across the 4 days indicated that SI males drank signifi cantly more than GH males, and this was not the case in females. Th ere was no signifi cant eff ect of housing for Hour 2. On Day 4, aft er 2 hours of consumption, social isolation increased drinking regardless of sex. Plasma corticosterone (CORT) levels decreased following drinking, but there were no sex or housing group diff erences. Th ere were correlations between CORT levels and drinking, but only for SI females. Th ese fi ndings demonstrate that adolescent social isolation promotes binge- like drinking in both male and female adult mice with a genetic predisposition for high alcohol preference; however, this relationship is time- dependent, and males may be more sensitive than females to social isolation stress. Additionally, corticosterone levels change with regard to binge- drinking and sex.
青少年压力暴露增加了成年后酒精滥用和酒精使用障碍(AUD)的可能性;然而,目前尚不清楚遗传和环境因素如何相互作用以增加风险。这项研究考察了青少年社会孤立如何影响成年后酗酒和应激激素皮质酮水平,这是在具有高酒精偏好(HAP)遗传倾向的雄性和雌性小鼠中进行的。将28只HAP小鼠分为群养(GH)和社会隔离(SI)两组(n = 13,13)。狂饮被评估使用饮酒在黑暗(DID)程序。在DID前和DID后第4天(最后一天)采集血样。总的来说,青少年的社会孤立增加了成人酗酒的性和时间依赖的方式。对4天内第1小时摄入量的分析表明,SI男性的饮酒量明显高于GH男性,而女性的情况并非如此。第2小时的住房没有显著的影响。在第4天,在饮酒2小时后,社交隔离增加了饮酒量,与性别无关。血浆皮质酮(CORT)水平在饮酒后下降,但没有性别或住房组差异。CORT水平与饮酒之间存在相关性,但仅适用于SI女性。这些发现表明,在具有高度酒精偏好的遗传倾向的雄性和雌性成年小鼠中,青春期的社会孤立都会促进狂欢式饮酒;然而,这种关系是时间依赖的,男性可能比女性对社会孤立压力更敏感。此外,皮质酮水平与酗酒和性行为有关。
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引用次数: 0
Navigating Political Disagreement in Social Work: An Analysis of Past Literature, Ethical Guidance, and Case Examples 引导社会工作中的政治分歧:对过去文献、伦理指导和案例的分析
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1572
Saige M. Addison
Political advocacy is a core tenet of the National Association of Social Workers (NASW) Code of Ethics. As a profession, social work has the responsibility to remain aware of trends that threaten the well- being of diverse populations. Th e historic connection between oppression and policy is undeniable, and the standards and principles in the Code of Ethics require social workers to intervene and be aware of political trends. However, at times, social workers’ political views may not refl ect the clients’ views. Navigating situations in which a client expresses political content with the social worker requires careful consideration and improvements in the available guidance. Prior literature addresses the political confl ict in Israel, Palestine, and Northern Ireland during times of extreme tension in those cultures. Yet, stark contrasts between the United States and those cultures exist, thus justifying the need for specifi c guidance for U.S. social workers. Currently, the state of society and social work in the United States requires social workers to dedicate increased attentiveness to these types of situations. As such, the connection between social work and political action is undeniable and worthy of further investigation. To do so, two relevant case examples from a licensed clinical social worker (LCSW) will be assessed in which guidance from the NASW Code of Ethics, existing approaches, and prior literature will be applied. Th e evaluation of these case examples is intended to inform the decisions of other U.S.- based social workers confronting political disagreement with clients in direct practice work.
政治宣传是全国社会工作者协会(NASW)道德准则的核心原则。作为一种职业,社会工作有责任保持对威胁不同人群福祉的趋势的意识。压迫与政策之间的历史联系是不可否认的,《道德准则》中的标准和原则要求社会工作者介入并了解政治趋势。然而,有时,社会工作者的政治观点可能不反映客户的观点。在客户向社会工作者表达政治内容的情况下,需要仔细考虑和改进现有的指导。先前的文献论述了以色列、巴勒斯坦和北爱尔兰在这些文化极端紧张时期的政治冲突。然而,美国与这些文化之间存在着鲜明的对比,因此有必要为美国社会工作者提供具体的指导。目前,美国的社会状况和社会工作要求社会工作者对这类情况给予更多的关注。因此,社会工作与政治行动之间的联系是不可否认的,值得进一步研究。为了做到这一点,我们将对两个相关的临床社会工作者(LCSW)的案例进行评估,其中将应用NASW道德准则、现有方法和先前文献的指导。对这些案例的评估旨在为其他美国社会工作者在直接实践工作中面对与客户的政治分歧时的决定提供信息。
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引用次数: 0
Comparison of College Students’ Perspectives of E-cigarette Use and Major of Study 大学生电子烟使用观点与所学专业的比较
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1529
Parker Woodruff
This study assesses college students’ ideals on safety, dis-tribution, and use of electronic nicotine delivery systems (ENDS). This research aims to identify trends in student perceptions of ENDS across different majors of study at Purdue University. This will allow us to identify opportu-nities to expand public health education regarding ENDS at Purdue University.
本研究评估大学生对电子尼古丁输送系统(ENDS)的安全性、分布和使用的理想。本研究旨在确定普渡大学不同专业学生对ENDS的看法的趋势。这将使我们能够确定在普渡大学扩大关于ENDS的公共卫生教育的机会。
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引用次数: 0
The Effect of Fatty Acid Synthase (FASN) Depletion in Pulmonary Metastatic Formation in Breast Cancer Murine Models 脂肪酸合酶(FASN)缺失在乳腺癌小鼠模型肺转移形成中的作用
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1549
Zilin Xianyu
Recent studies show that the five-year survival rate for women with metastatic (spreading) breast cancer is 28% whereas this number for women with nonmetastatic invasive breast cancer is 90%. Therefore, prevention of metastatic formation can be an effective way to reduce the mortality rate for breast cancer patients. Epithelial-mesenchymal transition (EMT) is a process that supports cancer to spread and is a vital mechanism that has been studied for decades. Through this process, epithelial (less mobile) cells can obtain mesenchymal (migratory) phenotypes to move from its original location to distant organs. Once they reach their destination, mesenchymal-epithelial transition (MET) will happen to help them become epithelial again to attach to the new epithelia. As a key enzyme in fatty acid production, fatty acid synthase (FASN) is increased with EMT induction and overexpressed in distant organ metastasis of breast cancer. As a result of that, we are interested in the influence of FASN in EMT in breast cancer metastasis. Our long-term goal is to develop FASN-targeting medications that can inhibit formation of pulmonary metastases originating from breast cancer cells. To achieve this goal, we will investigate the influence of a lack of FASN in pulmonary metastasis formation in breast cancer murine (mice) models. Our central hypothesis is that FASN is a vital protein for breast cancer metastasis, so a lack of FASN will inhibit the formation of pulmonary metastasis. Through this project, we hope to obtain a deeper understanding of FASN and its role in EMT to provide more options for breast cancer treatments.
最近的研究表明,患有转移性(扩散性)乳腺癌的妇女的五年生存率为28%,而患有非转移性浸润性乳腺癌的妇女的五年生存率为90%。因此,预防转移形成是降低乳腺癌患者死亡率的有效途径。上皮-间质转化(Epithelial-mesenchymal transition, EMT)是一个支持癌症扩散的过程,是一个重要的机制,已经被研究了几十年。通过这一过程,上皮细胞(流动性较差)可以获得间充质(迁移)表型,从其原始位置移动到远处的器官。一旦它们到达目的地,就会发生间充质-上皮转化(MET),帮助它们再次成为上皮细胞并附着在新的上皮细胞上。脂肪酸合成酶(fatty acid synthase, FASN)作为脂肪酸生成的关键酶,随着EMT的诱导而升高,并在乳腺癌远端器官转移中过表达。因此,我们对EMT中FASN对乳腺癌转移的影响感兴趣。我们的长期目标是开发针对fasn的药物,可以抑制起源于乳腺癌细胞的肺转移的形成。为了实现这一目标,我们将研究缺乏FASN对乳腺癌小鼠模型肺转移形成的影响。我们的中心假设是FASN是乳腺癌转移的重要蛋白,因此缺乏FASN会抑制肺转移的形成。我们希望通过这个项目更深入地了解FASN及其在EMT中的作用,为乳腺癌治疗提供更多的选择。
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引用次数: 0
Light Curve Inversion: Determining the Shape of Human-Made Space Objects 光曲线反演:确定人造空间物体的形状
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1588
Liam Robinson
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引用次数: 0
Microaggression Among Registered Nurses: An Exploratory Study 注册护士微攻击行为的探索性研究
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1574
Saige M. Addison
In this mixed method study, RNs (N = 22) completed the “Acceptability of Racial Microaggressions Scale” (ARMS, based on Mekawi and Todd’s 2018 research) before reading a vignette depicting a Black man in the emergency room. Subsequently, participants responded to three open-ended questions. Results revealed that there was overall low agreement with the ARMS subscales, indicating that the participants were generally unlikely to endorse microaggression; however, the Color Evasion subscale had the greatest agreement (M = 2.701). This suggests a greater adherence to colorblind beliefs and ideology than other forms of microaggression within the sample. The open-ended responses generated three themes: helpfulness, emotional (in)attentiveness, and emergency response. Helpfulness included a desire to assist the patient and obtain the patient’s compliance. Emotional (in)attentiveness was characterized by observations of the patient’s emotions, yet a subsequent lack of acknowledgment of the patient’s feelings. Finally, emergency response encompassed the medical observations and recommendations of the RNs.
在这项混合方法研究中,注册护士(N = 22)完成了“种族微侵犯可接受性量表”(ARMS,基于Mekawi和Todd 2018年的研究),然后阅读了一幅描绘急诊室黑人男子的小插图。随后,参与者回答了三个开放式问题。结果显示,被试与ARMS量表的一致性总体较低,表明被试一般不太可能认可微攻击行为;逃避颜色分量表的一致性最高(M = 2.701)。这表明,在样本中,与其他形式的微侵犯相比,他们更坚持色盲的信仰和意识形态。开放式的回答产生了三个主题:乐于助人、情感关注和紧急反应。乐于助人包括帮助病人的愿望和获得病人的顺从。情感关注的特点是观察病人的情绪,但随后缺乏对病人感受的承认。最后,应急反应包括注册护士的医疗观察和建议。
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引用次数: 0
Behavioral Alterations Following Exposure to a Mixture of Lead and Atrazine During Early Development in the Zebrafish Model System 斑马鱼模型系统早期发育过程中暴露于铅和阿特拉津混合物后的行为改变
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1564
Anusha KV Lakshmi Dharmavathi
Lead (Pb) and atrazine (ATZ) are hazardous environmental toxicants that can exist as a mixture in potable water. Numerous single chemical studies on Pb and ATZ support adverse health outcomes, including neurotoxicity, but mixture studies are limited. Th e aim of this study is to test the hypothesis that Pb and ATZ mixtures result in a greater than additive toxicity, causing increased adverse health outcomes than single chemical exposures. Th e zebrafi sh model system was exposed to 3 or 30 ppb (µg/L) ATZ, 10 ppb (equivalent to 0.1 µg/dL) or 100 ppb Pb, along with mixtures of each beginning at 1 hour postfertilization (hpf) and continuing through 120 hpf. A visual motor response behavioral assay was then conducted using the Noldus DanioVision. Th e visual motor response assay tracks locomotor movement over fi ve alternating periods of dark and light. Th e behavior data was analyzed by phase with a repeated measures ANOVA. Th e 10 ppb Pb mixture behavior data showed signifi cant hypoactivity in the 10 ppb Pb, 30 ppb ATZ, 3 ppb ATZ/10 ppb Pb, and 30 ppb ATZ/10 ppb Pb treatments for all parameters in multiple phases ( p < 0.05). Th e 100 ppb Pb mixture behavior data also showed signifi cant hypoactivity in the 100 ppb Pb and 30 ppb ATZ treatment groups, along with hypoactivity in both mixture treatment groups ( p < 0.05). Additionally, the 3 ppb ATZ treatment group had hyperactivity in the third dark phase ( p < 0.05). Th ese data align with past single chemical behavior studies and indicate a greater than additive mixture toxicity. zebrafish model system. Her dissertation has three main components, including predictive mixture modeling, molecular and cerebral vasculature development after mixture exposure, and generation of a disease model of neurodegener-ation using CRISPR- Cas9, a gene- editing technology. model system. These studies are investigating a developmental origin of adult disease pathogenesis with a specific focus on neuroendocrine dysfunction, neurodegenerative diseases, and cancer with a goal of understanding the role of exposure to the environmental chemicals in these adverse health outcomes. Behavioral Alterations following Exposure to a Mixture of Lead and Atrazine during Early Development in the Zebrafish Model System
铅(Pb)和阿特拉津(ATZ)是危险的环境毒物,可以作为混合物存在于饮用水中。许多关于铅和ATZ的单一化学研究支持不良健康结果,包括神经毒性,但混合研究有限。本研究的目的是验证这样一种假设,即铅和ATZ混合物的毒性大于添加剂,比单一化学品接触造成的不良健康后果更多。斑马鱼模型系统暴露于3或30 ppb(µg/L) ATZ, 10 ppb(相当于0.1µg/dL)或100 ppb Pb,以及每种混合物在受精后1小时(hpf)开始并持续到120 hpf。然后使用Noldus DanioVision进行视觉运动反应行为测试。视觉运动反应试验跟踪运动在五个交替的黑暗和光明时期。行为数据采用重复测量方差分析分阶段分析。10 ppb Pb混合行为数据显示,10 ppb Pb、30 ppb ATZ、3 ppb ATZ/10 ppb Pb和30 ppb ATZ/10 ppb Pb处理各阶段均显著降低活性(p < 0.05)。100 ppb Pb混合物的行为数据也显示100 ppb Pb和30 ppb ATZ处理组存在显著的低活性,两种混合物处理组均存在低活性(p < 0.05)。此外,3 ppb ATZ治疗组在第三暗期出现多动(p < 0.05)。这些数据与过去的单一化学行为研究一致,表明比添加剂混合物的毒性更大。斑马鱼模型系统。她的论文主要由三个部分组成,包括预测混合物建模,混合物暴露后的分子和脑血管系统发育,以及使用基因编辑技术CRISPR- Cas9生成神经变性疾病模型。模型系统。这些研究正在调查成人疾病发病机制的发育起源,特别关注神经内分泌功能障碍、神经退行性疾病和癌症,目的是了解暴露于环境化学物质在这些不良健康结果中的作用。斑马鱼模型系统早期发育过程中暴露于铅和阿特拉津混合物后的行为改变
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引用次数: 0
Interview: Cody Mullen 采访:科迪·马伦
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1592
Catie Gilhooly
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引用次数: 0
Non Ennarabile Textum: Allusive Ekphrasis in Francisco Javier Alegre's Alexandrias
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1544
Shashank Dimri
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引用次数: 0
Exploring the Purdue Teacher Education Program: Focus on Diversity, Equity, and Social Justice 探索普渡大学教师教育计划:关注多样性、公平和社会正义
Pub Date : 2022-11-01 DOI: 10.7771/2158-4052.1558
J. Pirrello
During this time of heightened awareness of social injustices via Black Lives Matter protests, the #MeToo movement, and the COVID- 19 pandemic, it is important to ensure that teacher preparation curriculum includes attention to knowledge and skills related to social justice issues in education. Th e purpose of this study was to investigate the ways in which social justice, diversity, and equity are addressed in the foundational courses of the Purdue Teacher Education Program (PTEP). McDonald (2005) proposed a framework in which social justice is integrated across all experiences (e.g., courses, activities, clinical placements) in a teacher education program. In an analysis of PTEP, we found that each required course referenced social justice, diversity, and/or equity, albeit to varying degrees, including practical applications of knowledge related to these ideas. In her framework, McDonald recommended conceptual and practical tools related to social justice. In PTEP, such tools (e.g., diff erentiation, Universal Design for Learning, culturally responsive teaching) are incorporated across multiple courses through readings and assignments. In this investigation, we used curricular analyses, faculty input, and student experiences to identify the nature of social justice references in the program curricula, and also highlight productive connections that begin in the foundations courses and could be continued into the methods courses later in the program. With a better understanding of the program curricula, personnel can both enhance opportunities in their own courses, as well as capitalize on experiences off ered in other courses.
在这个通过“黑人的命也是命”抗议、“我也是”运动和2019冠状病毒病(COVID- 19)大流行提高人们对社会不公正认识的时代,重要的是要确保教师备课课程包括对教育中社会正义问题相关知识和技能的关注。本研究的目的是探讨普渡大学教师教育计划(PTEP)的基础课程如何解决社会正义、多样性和公平问题。McDonald(2005)提出了一个框架,在这个框架中,社会正义被整合到教师教育项目的所有经历中(例如,课程、活动、临床实习)。在对PTEP的分析中,我们发现每个必修课程都涉及到社会正义、多样性和/或公平,尽管程度不同,包括与这些理念相关的知识的实际应用。在她的框架中,麦克唐纳推荐了与社会正义相关的概念和实用工具。在PTEP中,这些工具(例如,差异化,学习通用设计,文化响应教学)通过阅读和作业整合到多个课程中。在这项调查中,我们使用课程分析、教师投入和学生经验来确定课程课程中社会正义参考的性质,并强调从基础课程开始的生产性联系,这些联系可以继续进入课程后期的方法课程。通过更好地了解项目课程,员工既可以在自己的课程中增加机会,也可以利用在其他课程中获得的经验。
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引用次数: 0
期刊
Journal of Purdue Undergraduate Research
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